Device for breeding and releasing parasitic wasps in egg stage of rice planthopper and application of device

Abstract

The invention belongs to the technical field of rice planthopper prevention and control, and particularly relates to a device for breeding and releasing parasitic wasps in the egg stage of rice planthoppers and application of the device. The device provided by the invention comprises a breeding element and a release element, wherein the breeding element comprises a first transparent container with an upper opening and a first transparent cover matched with the first transparent container with the upper opening; at least one ventilation element is arranged on the first transparent cover; the release element comprises a degradable material container with an opening in the upper portion and a funnel-shaped cover matched with the degradable material container with the opening in the upper portion. The device is simple in structure, the whole breeding process of parasitic wasps in the egg stage of rice planthoppers can be completed through the breeding element, due to phototaxis, the parasitic wasps directly fly out of the funnel-shaped cover after eclosion, rice planthopper eggs are found for parasitism, rice planthoppers are prevented and controlled, and if nymphs of brown planthoppers which are not parasitized in the breeding element are hatched, the rice planthoppers are not parasitized. As the rice cannot climb out of the releasing device, the rice cannot be harmed to the rice in the rice field.

Description

Technical Field

[0001] The present invention belongs to the technical field of rice planthopper prevention and control, and particularly relates to a device for breeding and releasing egg-stage parasitic wasps of rice planthoppers and its application. Background Art

[0002] As one of the most important food crops in the world, rice occupies an important position in agricultural production. Rice planthoppers are one of the main pests in rice cultivation, belonging to the family Delphacidae of the order Hemiptera. Their damage methods mainly include directly sucking and feeding on the phloem of rice, causing mechanical damage to rice during the processes of sucking and oviposition, and contaminating rice with secretions and honeydew. In addition, rice planthoppers are important vectors for rice virus diseases.

[0003] Egg-stage parasitic natural enemies of rice planthoppers play a significant role in controlling the occurrence of rice planthoppers, mainly including Anagrus spp., Ollgosita spp., etc. Among them, Anagrus nilaparvatae belongs to the genus Anagrus of the family Mymaridae and is one of the egg-stage parasitic natural enemies of rice planthoppers. The highest parasitism rate of rice planthoppers can reach over 80%, which is a key biological factor for controlling the population of rice planthoppers in the field. However, the natural population growth of Anagrus nilaparvatae in paddy fields lags behind that of rice planthoppers. In the initial stage when rice planthoppers first migrate into paddy fields, the number of Anagrus nilaparvatae is relatively small, making it difficult to effectively control the population growth of rice planthoppers. In order to make up for the deficiency of the natural population of Anagrus nilaparvatae at this time, artificially breeding a large number of Anagrus nilaparvatae and releasing them into the field is expected to achieve the purpose of effectively controlling pests through biological control means. Summary of the Invention

[0004] The purpose of the present invention is to provide a device for breeding and releasing egg-stage parasitic wasps of rice planthoppers and its application, to achieve the large-scale and rapid reproduction and release of egg-stage parasitic wasps of rice planthoppers, significantly improve the parasitism rate of rice planthopper eggs in the field, effectively control the population of rice planthoppers, and effectively prevent and control rice planthoppers.

[0005] The present invention provides a device for breeding and releasing egg-stage parasitic wasps of rice planthoppers, including a breeding element and a release element;

[0006] The breeding element includes a first breeding element; the first breeding element includes a first transparent container with an upper opening and a first transparent lid that matches the first transparent container with the upper opening; at least one air-permeable element is provided on the first transparent lid;

[0007] The release element includes a degradable material container with an upper opening and a funnel-shaped lid that matches the degradable material container with the upper opening; the opening angle of the funnel-shaped lid inside the cylinder is ≥ 45°.

[0008] Preferably, the materials of the first transparent container with an upper opening and the first transparent lid respectively include polyester; the degradable material includes rice husk fiber.

[0009] Preferably, the height of the first transparent container with an upper opening is 230 - 260 mm, and the diameter is 80 - 120 mm;

[0010] The breathable element includes a sieve; the diameter of the sieve is 50 - 70 mm; the particle size of the pores on the sieve is 200 - 250 mesh;

[0011] The height of the degradable material container with an upper opening is 220 - 240 mm, and the diameter is 100 - 110 mm;

[0012] The height of the funnel-shaped lid is 25 - 35 mm, and the aperture is 8 - 10 mm.

[0013] Preferably, the breeding element further includes a second breeding element and a collection element;

[0014] The second breeding element includes an opaque container with an upper opening and an opaque lid that matches the opaque container with the upper opening;

[0015] The collection element includes a second transparent container with an upper opening and a second transparent lid that matches the second transparent container with the upper opening;

[0016] The second transparent container with an upper opening includes a first cavity and a second cavity; the diameter of the first cavity ≥ the diameter of the second cavity, and the second cavity has an upper opening;

[0017] The opaque container with an upper opening and the first transparent container with an upper opening can form a nested doll structure with the first transparent container with an upper opening inside and the opaque container with an upper opening outside;

[0018] The opaque lid is provided with a through hole; the diameter of the through hole ≥ the diameter of the second cavity and < the diameter of the first cavity;

[0019] The second transparent lid is provided with ventilation holes.

[0020] Preferably, the materials of the opaque container with an upper opening and the opaque lid respectively include plastic; the material of the second transparent container with an upper opening includes glass.

[0021] Preferably, the height of the opaque container with an upper opening is 235 - 265 mm, and the diameter is 85 - 125 mm;

[0022] The diameter of the through hole on the opaque lid is 25 - 30 mm;

[0023] The height of the second transparent container with an upper opening is 70 - 80 mm, and the diameter of the first cavity is 30 - 35 mm;

[0024] The diameter of the ventilation holes provided on the second transparent lid is 10 - 15 mm.

[0025] The present invention also provides an application of the device according to the above technical solution in one or more of the following:

[0026] (1) Breeding and releasing of egg - stage parasitoids of rice planthoppers;

[0027] (2) Remote transportation of egg - stage parasitoids of rice planthoppers;

[0028] (3) Field control of rice planthoppers.

[0029] Preferably, the field control of rice planthoppers includes increasing the parasitism rate of parasitoids in the eggs of rice planthoppers in the field and / or reducing the population number of added rice planthoppers.

[0030] The present invention also provides a method for field control of rice planthoppers based on the device according to the above technical solution, including the following steps:

[0031] Cultivate rice in the first transparent container with an upper opening to obtain a first transparent container containing rice seedlings;

[0032] Inoculate adult rice planthoppers into the first transparent container containing rice seedlings, and after the adult rice planthoppers lay eggs, obtain a first transparent container containing eggs of rice planthoppers;

[0033] Inoculate egg - stage parasitoids of rice planthoppers into the first transparent container containing eggs of rice planthoppers to obtain a first transparent container containing rice seedlings parasitized by parasitoids;

[0034] Transfer the rice seedlings parasitized by parasitoids in the first transparent container containing rice seedlings parasitized by parasitoids to a container made of degradable material with an upper opening, cover the funnel - shaped lid, and transport it to the field to be controlled.

[0035] Preferably, before the hatching of the egg - stage parasitoids of rice planthoppers, it further includes:

[0036] Put the opaque container with an upper opening outside the first transparent container containing rice seedlings parasitized by parasitoids, cover the opaque lid, insert the second transparent container with an upper opening downward into the through - hole of the opaque lid to obtain a breeding container containing rice seedlings parasitized by parasitoids; cultivate the breeding container containing rice seedlings parasitized by parasitoids;

[0037] The temperature of the cultivation is 24.0 - 26.0 °C, the relative humidity is 70 - 90%, and the photoperiod is 12D:12L.

[0038] Beneficial effects:

[0039] The present invention provides a device for breeding and releasing egg-stage parasitic wasps of rice planthoppers, comprising a breeding element and a releasing element; the breeding element includes a first breeding element; the first breeding element includes a first transparent container with an upper opening and a first transparent lid matching the first transparent container with the upper opening; at least one air-permeable element is arranged on the first transparent lid; the releasing element includes a degradable material container with an upper opening and a funnel-shaped lid matching the degradable material container with the upper opening. The device of the present invention has a simple structure. The whole breeding process of egg-stage parasitic wasps of rice planthoppers can be completed by using the breeding element. Due to phototaxis, after the parasitic wasps emerge as adults, they directly fly out from the funnel-shaped lid to look for rice planthopper eggs for parasitism, thereby controlling rice planthoppers in paddy fields. If the unparasitized brown planthoppers in the breeding element hatch into nymphs, since they cannot climb out of the releasing device, they will not cause harm to the rice in the paddy field. Moreover, the releasing element of the present invention adopts disposable degradable materials, effectively reducing the artificial recovery and treatment costs.

[0040] Furthermore, in the breeding element of the present invention, the second breeding element adopts an opaque container with an upper opening, and can form a nested structure with the first transparent container with the upper opening, with the first transparent container with the upper opening inside and the opaque container with the upper opening outside, which is convenient for disassembly and can be recycled. After the egg-stage parasitic wasps of rice planthoppers emerge as adults, insert the second transparent container with the upper opening into the through hole on the opaque lid. By using the phototaxis of the egg-stage parasitic wasps of rice planthoppers, gather them into the second transparent container with the upper opening, and cover the lid, realizing the enlarged cultivation of the egg-stage parasitic wasps of rice planthoppers. The collected egg-stage parasitic wasps of rice planthoppers can be used for subculture or experimental research. Brief description of the drawings

[0041] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required to be used in the embodiments.

[0042] Figure 1 It is the front view of the first breeding element in Embodiment 1; among them, the left side is the combined drawing of each component of the first breeding element, and the right side is the exploded view of each component of the first breeding element;

[0043] Figure 2 It is the front view after the components of the second breeding element in Embodiment 1 are combined;

[0044] Figure 3 It is the front view of the collection element in Embodiment 1; among them, the left side is the combined drawing of each component of the collection element, and the right side is the exploded view of each component of the collection element;

[0045] Figure 4Front view of the release element in Example 1; where the left side is a combined view of the components of the release element, and the right side is a disassembled view of the components of the release element;

[0046] Figure 5 Front view of the components of the breeding element in Example 1 after combination. Detailed implementation manners

[0047] The present invention provides a device for breeding and releasing egg-stage parasitic wasps of rice planthoppers, comprising a breeding element and a release element;

[0048] The breeding element includes a first breeding element; the first breeding element includes a first transparent container with an upper opening and a first transparent lid that matches the first transparent container with the upper opening; at least one air-permeable element is provided on the first transparent lid;

[0049] The release element includes a degradable material container with an upper opening and a funnel-shaped lid that matches the degradable material container with the upper opening; the opening angle of the funnel-shaped lid inside the cylinder is ≥45°.

[0050] As an implementation manner, the material of the first transparent container with the upper opening in the present invention is polyester; as another implementation manner, the material of the first transparent container with the upper opening in the present invention is polyester film. Using polyester as the material in the present invention has the advantages of being lightweight, durable, having a smooth surface and being easy to clean, being reusable, and having a relatively low cost. As an implementation manner, the height of the first transparent container with the upper opening in the present invention is 230 - 260 mm; as another implementation manner, the height of the first transparent container with the upper opening in the present invention is 250 mm. As an implementation manner, the diameter of the first transparent container with the upper opening in the present invention is 80 - 1200 mm; as another implementation manner, the diameter of the first transparent container with the upper opening in the present invention is 100 mm. As an implementation manner, the shape of the first transparent container with the upper opening in the present invention is cylindrical. Limiting the specifications of the first transparent container with the upper opening in the present invention has the advantages of being lightweight, durable, having a smooth surface and being easy to clean, being reusable, and having a relatively low cost.

[0051] As an implementation manner, the material of the first transparent lid in the present invention includes polyester. As an implementation manner, the air-permeable element includes a screen. As an implementation manner, the diameter of the screen in the present invention is 50 - 70 mm; as another implementation manner, the diameter of the screen in the present invention is 60 mm. As an implementation manner, the particle size of the pores on the screen in the present invention is 200 - 250 mesh; as another implementation manner, the particle size of the pores on the screen in the present invention is 200 mesh.

[0052] As an implementation manner, the breeding element of the present invention further includes a second breeding element and a collection element; the second breeding element includes an opaque container with an upper opening and an opaque lid that matches the opaque container with the upper opening; the collection element includes a second transparent container with an upper opening and a second transparent lid that matches the second transparent container with the upper opening.

[0053] As an implementation manner, the opaque container with an upper opening and the first transparent container with an upper opening of the present invention can form a nested structure with the first transparent container with an upper opening inside and the opaque container with an upper opening outside. As an implementation manner, the material of the opaque container with an upper opening of the present invention includes plastic; as an implementation manner, the material of the opaque container with an upper opening of the present invention includes black light-blocking plastic. Using black light-blocking plastic as the material has the advantages of strong light-shielding property, light weight, durability, easy disassembly, and low cost. As an implementation manner, the height of the opaque container with an upper opening of the present invention is 235-265 mm; as another implementation manner, the height of the opaque container with an upper opening of the present invention is 255 mm. As an implementation manner, the diameter of the opaque container with an upper opening of the present invention is 85-125 mm; as another implementation manner, the diameter of the opaque container with an upper opening of the present invention is 105 mm. Defining the specifications of the opaque container with an upper opening of the present invention has the advantages of strong light-shielding property, light weight, durability, easy disassembly, and low cost.

[0054] As an implementation manner, the second transparent container with an upper opening of the present invention includes a first cavity and a second cavity; the diameter of the first cavity ≥ the diameter of the second cavity, and the second cavity has an upper opening. As an implementation manner, the height of the second transparent container with an upper opening of the present invention is 70-80 mm; as another implementation manner, the height of the second transparent container with an upper opening of the present invention is 80 mm. As an implementation manner, the diameter of the first cavity is 30-35 mm; as another implementation manner, the diameter of the first cavity of the present invention is 30 mm. As an implementation manner, the diameter of the second cavity is 25-30 mm. As an implementation manner, the second cavity is provided with a threaded structure and is threadedly connected to the second transparent lid. Defining the specifications of the second transparent container with an upper opening of the present invention has the advantages of high transparency, easy observation of the insect situation, recyclability, and low cost.

[0055] As an implementation manner, the opaque lid of the present invention is provided with a through hole; the diameter of the through hole is ≥ the diameter of the second cavity and < the diameter of the first cavity. As an implementation manner, the material of the opaque lid of the present invention includes plastic; as an implementation manner, the material of the opaque lid of the present invention includes black opaque plastic. Using black opaque plastic as the material has the advantages of strong light-shielding property, light weight, durability, easy disassembly, and low cost. As an implementation manner, the diameter of the through hole on the opaque lid of the present invention is 25 - 30 mm; as another implementation manner, the diameter of the through hole on the opaque lid of the present invention is 25 mm.

[0056] As an implementation manner, the second transparent lid of the present invention is provided with ventilation holes. As an implementation manner, the diameter of the ventilation holes is 10 - 15 mm; as another implementation manner, the ventilation holes of the present invention are 10 mm.

[0057] The device of the present invention includes a release element; the release element includes a degradable material container with an upper opening and a funnel-shaped lid that matches the degradable material container with the upper opening; the opening angle of the funnel-shaped lid in the cylinder is ≥ 45°.

[0058] As an implementation manner, the height of the degradable material container with the upper opening of the present invention is 220 - 240 mm; as another implementation manner, the height of the degradable material container with the upper opening of the present invention is 230 mm. As an implementation manner, the diameter of the degradable material container with the upper opening of the present invention is 100 - 110 mm; as another implementation manner, the diameter of the degradable material container with the upper opening of the present invention is 100 mm. As an implementation manner, the degradable material includes rice husk fiber. Using rice husk fiber as the material has the advantages of environmental protection and degradability, reducing the cost of manual recycling and treatment, low cost, and being suitable for large-scale use.

[0059] As an implementation manner, the height of the funnel-shaped lid of the present invention is 25 - 35 mm; as another implementation manner, the height of the funnel-shaped lid of the present invention is 30 mm. As an implementation manner, the aperture of the funnel-shaped lid of the present invention is 8 - 10 mm; as another implementation manner, the aperture of the funnel-shaped lid of the present invention is 10 mm. As an implementation manner, the material of the funnel-shaped lid of the present invention includes rice husk fiber. Using rice husk fiber as the material has the advantages of environmental protection and degradability, reducing the cost of manual recycling and treatment, low cost, and being suitable for large-scale use.

[0060] The device of the present invention has a simple structure, which is convenient for the breeding, long-distance transportation, and field release of Anagrus nilaparvatae.

[0061] In view of the advantages of the device provided by the present invention, the application of the device in one or more of (1) breeding and releasing egg-stage parasitoids of rice planthoppers; (2) long-distance transportation of egg-stage parasitoids of rice planthoppers; and (3) field control of rice planthoppers also falls within the protection scope of the present invention.

[0062] As an implementation mode, the field control of rice planthoppers in the present invention includes increasing the parasitism rate of parasitoids in the eggs of rice planthoppers in the field and / or reducing the population number of added rice planthoppers. The rice planthoppers in the present invention include brown planthoppers.

[0063] The present invention also provides a method for field control of rice planthoppers based on the device according to the above technical solution, including the following steps:

[0064] Cultivate rice in the first transparent container with an upper opening to obtain a first transparent container containing rice seedlings;

[0065] Inoculate adult rice planthoppers into the first transparent container containing rice seedlings, and after the adult rice planthoppers lay eggs, obtain a first transparent container containing rice planthopper eggs;

[0066] Inoculate egg-stage parasitoids of rice planthoppers into the first transparent container containing rice planthopper eggs to obtain a first transparent container containing parasitoid-parasitized rice seedlings;

[0067] Transfer the parasitoid-parasitized rice seedlings in the first transparent container containing parasitoid-parasitized rice seedlings to a degradable material container with an upper opening, cover the funnel-shaped lid, and transport it to the field to be controlled.

[0068] The present invention cultivates rice in the first transparent container with an upper opening to obtain a first transparent container containing rice seedlings. As an implementation mode, the present invention sows germinated rice seeds after adding a seedling-raising substrate to the first transparent container with an upper opening, and covers the first transparent lid for cultivation. As an implementation mode, the cultivation conditions in the present invention are: temperature 24.0 - 26.0 °C, relative humidity 70 - 90%, photoperiod 12D:12L; as another implementation mode, the cultivation conditions in the present invention are: temperature 25.0 °C, relative humidity 70 - 90%, photoperiod 12D:12L. As an implementation mode, the sowing amount of the rice seeds in the present invention is 20 - 25 grains per first transparent container with an upper opening.

[0069] After obtaining the first transparent container containing rice seedlings, the present invention inoculates adult rice planthoppers into the first transparent container containing rice seedlings, and after the adult rice planthoppers lay eggs, obtains a first transparent container containing rice planthopper eggs.

[0070] As an implementation mode, the inoculation ratio of the adult rice planthoppers in the present invention is 10 to 20 individuals per first transparent container containing rice seedlings. As an implementation mode, the first transparent container containing rice planthopper eggs is obtained 24 hours after the adult rice planthoppers lay eggs in the present invention. The present invention strictly limits the inoculation ratio of the adult rice planthoppers, and has the advantage of improving the parasitism efficiency.

[0071] After obtaining the first transparent container containing rice planthopper eggs, the present invention inoculates the egg-stage parasitoid of the rice planthopper into the first transparent container containing rice planthopper eggs to obtain a first transparent container containing parasitoid-parasitized rice seedlings.

[0072] As an implementation mode, the egg-stage parasitoid of the rice planthopper in the present invention includes Anagrus nilaparvatae. As an implementation mode, the inoculation ratio of the egg-stage parasitoid of the rice planthopper in the present invention is 20 to 50 individuals per first transparent container containing rice planthopper eggs; as another implementation mode, the inoculation ratio of the egg-stage parasitoid of the rice planthopper in the present invention is 30 to 40 individuals per first transparent container containing rice planthopper eggs. As an implementation mode, the male-female ratio of the inoculation of the egg-stage parasitoid of the rice planthopper in the present invention is 1:0.2 to 0.6; as another implementation mode, the male-female ratio of the inoculation of the egg-stage parasitoid of the rice planthopper in the present invention is 1:0.3 to 0.5; as an implementation mode, the male-female ratio of the inoculation of the egg-stage parasitoid of the rice planthopper in the present invention is 1:0.4. The present invention strictly limits the inoculation ratio of the egg-stage parasitoid of the rice planthopper, and has the advantage of improving the parasitism efficiency.

[0073] As an implementation mode, before the hatching of the egg-stage parasitoid of the rice planthopper in the present invention, the opaque container with an upper opening is sleeved outside the first transparent container containing parasitoid-parasitized rice seedlings, the opaque lid is covered, and the second transparent container with an upper opening is inserted downward into the through hole of the opaque lid to obtain a breeding container containing parasitoid-parasitized rice seedlings; the breeding container containing parasitoid-parasitized rice seedlings is cultured. As an implementation mode, the conditions for the culture in the present invention are: temperature 24.0 to 26.0 °C, relative humidity 70 to 90%, photoperiod 12D:12L; as another implementation mode, the conditions for the culture in the present invention are: temperature 25.0 °C, relative humidity 70 to 90%, photoperiod 12D:12L.

[0074] A first transparent container containing rice seedlings parasitized by parasitic wasps is obtained. In the present invention, the rice seedlings parasitized by parasitic wasps in the first transparent container are transferred into a degradable material container with an open top, the funnel-shaped lid is covered, and it is transported to the field to be controlled. As an implementation method, in the field to be controlled, 3 - 5 degradable material containers containing rice seedlings parasitized by parasitic wasps are placed per mu; as another implementation method, in the field to be controlled, 3 degradable material containers containing rice seedlings parasitized by parasitic wasps are placed per mu. As an implementation method, the distance between two adjacent degradable material containers containing rice seedlings parasitized by parasitic wasps is ≥20 cm; as another implementation method, the distance between two adjacent degradable material containers containing rice seedlings parasitized by parasitic wasps is 20 cm. In the present invention, the degradable material container containing rice seedlings parasitized by parasitic wasps is placed in the field to be controlled. After the parasitic wasps emerge, they fly out of the device due to phototaxis and fly to the paddy field to look for planthopper eggs to parasitize, thereby controlling the planthoppers in the paddy field. And the unparasitized brown planthoppers in the device, if they hatch into nymphs, will not harm the rice in the paddy field because they cannot climb out of the release device.

[0075] To further illustrate the present invention, the following describes in detail a device for breeding and releasing parasitic wasps during the egg stage of rice planthoppers and its application provided by the present invention in conjunction with the drawings and embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0076] Example 1

[0077] A device for breeding and releasing parasitic wasps during the egg stage of rice planthoppers consists of a breeding element and a releasing element;

[0078] The breeding element consists of a first breeding element, a second breeding element, and a collecting element;

[0079] The first breeding element consists of a cylindrical polyester film tube (i.e., a first transparent container with an open top) and a colorless transparent polyester lid (a first transparent lid matching the first transparent container with an open top), as shown in the schematic diagram Figure 1 shown. Among them, the overall cylindrical polyester film tube is 250 mm high and 100 mm in diameter, specifically colorless and transparent, with an open lid on the upper side and a sealed bottom cylinder; there is a breathable mesh in the middle of the colorless transparent polyester lid, the diameter of the mesh is 60 mm, and the particle size is 200 mesh;

[0080] The second breeding element consists of a black light-tight tube (i.e., an opaque container with an open top) and an opaque plastic lid (an opaque lid matching the opaque container with an open top), as shown in the schematic diagram Figure 2As shown in the figure. Among them, the overall black light-tight cylinder is a black light-tight plastic cylinder with a height of 255 mm and a diameter of 105 mm. It has an opening on the upper side and is sealed on the lower side, and is used to cover the outer side of the transparent cylindrical polyester film cylinder for shading; the light-tight plastic cover is covered with a black plastic film and has a hole with a diameter of 25 mm.

[0081] The collection element is composed of a glass tube (i.e., a second transparent container with an opening on the upper side) and a lid (i.e., a second transparent lid that matches the second transparent container with the opening on the upper side). The schematic diagram is as Figure 3 shown in the figure. Among them, the glass tube includes a first cavity and a second cavity. The overall height of the glass tube is 80 mm, the diameter of the first cavity is 30 mm; the diameter of the second cavity is 25 mm, and it is provided with a threaded structure and is threadedly connected to the lid; there is a vent hole with a diameter of 10 mm on the lid.

[0082] The release element is composed of a degradable plastic cylinder (i.e., a container made of degradable material with an opening on the upper side) and a funnel-shaped lid. The schematic diagram is as Figure 4 shown in the figure. Among them, the opening angle of the funnel-shaped lid inside the cylinder is ≥45°. The material of the degradable plastic cylinder is rice husk fiber, with an overall height of 230 mm and a diameter of 100 mm. The height of the funnel-shaped lid is 30 mm, and there is a 10 mm opening on the lid, so that the Anagrus nilaparvatae can fly out of the device to achieve the purpose of release.

[0083] Example 2

[0084] A method for breeding and field release of Anagrus nilaparvatae consists of the following steps:

[0085] (1) Put 2 cm thick moist seedling-raising substrate (the mass ratio of PINDSTRUP peat soil to Yoshida rice nutrient solution is 2:3, relative humidity 60 - 80%) in the cylindrical polyester film cylinder of Example 1. Among them, the preparation method of Yoshida rice nutrient solution is: weigh 0.575 g of rice nutrient solution (dry powder) (Alishan (Guangzhou) Biotechnology Co., Ltd.), add 100 g of water and stir. After complete dissolution, make up the volume to 1000 ml and shake well for use). After the rice seeds are cleaned of impurities and empty grains, they are soaked in clean water. After 24 hours, pour out the clean water and cover them with a wet gauze for germination. After the rice seeds show white tips, they are evenly sown in the moist seedling-raising substrate in the cylindrical polyester film cylinder, and covered with a layer of 0.5 cm thick dry peat soil (ensure that all rice seeds are covered with dry peat soil). After compaction, cover it with a colorless transparent polyester cover with a ventilation mesh in the middle, and put it into a constant temperature incubator (temperature 25.0 ± 1°C, relative humidity 70 - 90%, photoperiod 12 h D:12 h L), and cultivate until the seedling stage (10 - 20 days after sowing) for feeding the planthoppers.

[0086] (2) Use a suction tube to introduce adult female rice planthoppers carrying eggs into the rice seedlings cultivated to the seedling stage in step (1). Introduce 10 into each cylinder, and mark the inoculation date of the adult rice planthoppers. After 24 hours of their oviposition, they are used for subsequent breeding of Anagrus nilaparvatae.

[0087] (3) Introduce adult Anagrus nilaparvatae into the cylindrical polyester film cylinder with rice planthopper eggs and seedlings in step (2). Introduce 30 Anagrus nilaparvatae into each cylinder, and the male-female ratio is 1:0.6. Mark the date of introducing the adult Anagrus nilaparvatae on the cylinder body.

[0088] (4) Indoor breeding

[0089] Five to six days after introducing Anagrus nilaparvatae in step (3), put the black light-proof cylinder in Example 1 outside the cylindrical polyester film cylinder, cover it with the light-proof plastic cover in Example 1, and place the glass tube mouth of Example 1 downward in the hole of the light-proof plastic cover ( Figure 5 ), and place this device in a constant temperature incubator or climate chamber (temperature 25.0 ± 1 °C, relative humidity 70 - 90%, photoperiod 12D:12L). After the Anagrus nilaparvatae emerge, use their phototaxis to gather in the glass tube, which can be used for subculture or experimental research.

[0090] (5) Field release

[0091] Five to six days after introducing Anagrus nilaparvatae in step (3), transfer the fully parasitized rice seedlings to the degradable plastic cylinder in Example 1 and transport them to the field, and place this degradable plastic cylinder in the paddy field. After the Anagrus nilaparvatae emerge, they fly out of the device due to phototaxis and fly to the paddy field to look for rice planthopper eggs to parasitize, thereby controlling the rice planthoppers in the paddy field. And if the unparasitized rice planthoppers in the device hatch into nymphs, they cannot climb out of this release element and will not harm the rice in the paddy field. It is advisable to have 3 - 5 release points per mu in the field release, and the distance between release points is more than 20 m.

[0092] Application Example 1

[0093] The experiment was conducted in the rice green prevention and control demonstration area of Shiping Village, Tangxi Town, Jinhua City, Zhejiang Province. The rice variety was Yongyou 1540, and the experimental area was 20 mu, divided into two treatments: the wasp release treatment (experimental group) and the conventional green prevention and control treatment (control group), each with 10 mu, and the distance between the two treatments was more than 100 m.

[0094] Experimental group: 30 days after rice transplanting, when the rice was in the tillering stage, select 3 release points on one side near the field ridge per mu of land, and the distance between adjacent two release points is 20 m. Transfer the rice seedlings parasitized by Anagrus nilaparvatae within 5 - 6 days in step (3) of Example 2 to the degradable plastic cylinder in Example 1, transport them to the field, and place this degradable plastic cylinder at the release point, and place 1 such degradable plastic cylinder at each release point.

[0095] Control group: 30 days after rice transplantation, when the rice was in the tillering stage, conventional chemical control was carried out.

[0096] Keep other farming operations of the experimental group and the control group the same.

[0097] Before the release in the experimental group, 30 days after the release, and 50 days after the release, the population numbers of rice planthoppers in the fields of the experimental group and the control group were investigated. Specifically: For each treatment, 5 evenly distributed fields were selected, 5 random points were selected in each field, and 10 rice hills were investigated at each point. The parallel jumping sampling method and the basin slapping method were used to investigate the number of rice planthoppers (including Laodelphax striatellus, Sogatella furcifera, and Nilaparvata lugens). At the same time, the egg trapping method was used to investigate the number of egg parasitoids of rice planthoppers. Specifically: For each treatment, three points were collected at a distance of 10 m from the field ridge, with an interval of >5 m between each point. A pot of rice seedlings with eggs of Nilaparvata lugens was placed (3 strong rice seedlings in each pot, inoculated with 5 gravid Nilaparvata lugens, and the Nilaparvata lugens were removed 24 h after oviposition). After 48 h, it was retrieved and taken back to the laboratory, and cultivated in a closed insect-free artificial climate chamber (26.0 ± 1 °C, 70% - 90% humidity (RH), 12D:12L light-dark ratio) for 5 d and then dissected to check the total number of eggs of Nilaparvata lugens and the number of parasitized eggs. The specific statistical results are shown in Table 1.

[0098] Table 1 Effects of field release of Anagrus nilaparvatae on the population numbers of rice planthoppers and the parasitism rate of Anagrus nilaparvatae

[0099]

[0100] Note: The data in the table are mean ± standard error; different letters after the data in the same column indicate significant differences (P < 0.05).

[0101] It can be seen from Table 1 that by field releasing Anagrus nilaparvatae, the parasitism rate of rice planthopper eggs in the field can be significantly increased, and the population numbers of rice planthoppers can be effectively controlled.

[0102] It can be seen from the above content that the present invention can achieve the large-scale and rapid reproduction and release of egg parasitoids of rice planthoppers, significantly increase the parasitism rate of rice planthopper eggs in the field, effectively control the population numbers of rice planthoppers, and effectively prevent and control rice planthoppers.

[0103] Although the above embodiments have made a detailed description of the present invention, they are only a part of the embodiments of the present invention, not all embodiments. People can also obtain other embodiments based on this embodiment without creative efforts, and these embodiments all belong to the protection scope of the present invention.

Claims

1. A device for breeding and releasing rice planthopper egg-stage parasitic wasps, characterized in that: Includes breeding components and release components; The breeding element comprises a first breeding element; the first breeding element comprises a first transparent container with an upper opening and a first transparent cover matched with the first transparent container with an upper opening; At least one air permeable element is disposed on the first transparent cover; The release element comprises a degradable material container with an upper opening and a funnel-shaped cover matched with the degradable material container with an upper opening; the opening angle of the funnel-shaped cover in the cylinder is ≥45°.

2. The device according to claim 1, characterized in that The materials of the first transparent container with an upper opening and the first transparent cover include polyester respectively; the degradable material includes rice husk fiber.

3. The device according to claim 1, characterized in that The first transparent container with an upper opening has a height of 230 to 260 mm and a diameter of 80 to 120 mm; The air permeable element includes a screen; the diameter of the screen is 50-70 mm; the particle size of the pores on the screen is 200-250 meshes; The height of the biodegradable material container with an opening on the top is 220-240 mm, and the diameter is 100-110 mm; The height of the funnel-shaped cover is 25 to 35 mm, and the hole diameter is 8 to 10 mm.

4. The device according to any one of claims 1 to 3, characterized in that: The breeding element further comprises a second breeding element and a collecting element; The second breeding element comprises an opaque container with an upper opening and an opaque cover matching the opaque container with an upper opening; The collecting element comprises a second transparent container with an upper opening and a second transparent cover matched with the second transparent container with an upper opening; The second transparent container with an opening at the top comprises a first cavity and a second cavity; the diameter of the first cavity is greater than or equal to the diameter of the second cavity, and the second cavity is open at the top; The opaque container with an upper opening and the first transparent container with an upper opening can form a nesting doll structure with the first transparent container with an upper opening inside and the opaque container with an upper opening outside; The opaque cover is provided with a through hole; the diameter of the through hole is ≥ the diameter of the second cavity and < the diameter of the first cavity; The second transparent cover is provided with ventilation holes.

5. The device according to claim 4, characterized in that The material of the opaque container with an upper opening and the opaque cover comprises plastic respectively; the material of the second transparent container with an upper opening comprises glass.

6. The device according to claim 4 or 5, characterized in that The height of the opaque container with an opening at the top is 235 to 265 mm, and the diameter is 85 to 125 mm; The diameter of the through hole on the opaque cover is 25 to 30 mm; The height of the second transparent container with an upper opening is 70 to 80 mm, and the diameter of the first cavity is 30 to 35 mm; The second transparent cover is provided with a ventilation hole with a diameter of 10 to 15 mm.

7. Use of the device according to any one of claims 1 to 6 in one or more of the following: (1) Breeding and release of parasitic wasps in the egg stage of rice planthoppers; (2) Long-distance transportation of rice planthopper egg-stage parasitic wasps; (3) Field control of rice planthoppers.

8. The use according to claim 7, characterized in that: The field control of rice planthoppers includes increasing the parasitism rate of parasitic wasps in rice planthopper eggs in the field and / or reducing the population of added rice planthoppers.

9. A method for controlling rice planthoppers in the field based on the device according to any one of claims 1 to 6, characterized in that: The steps include: Cultivating rice in the first transparent container with an upper opening to obtain a first transparent container containing rice seedlings; Inoculating rice planthopper adults into the first transparent container containing rice seedlings, and obtaining a first transparent container containing rice planthopper eggs after the rice planthopper adults lay eggs; Inoculating a parasitic wasp in the egg stage of a rice planthopper into the first transparent container containing rice planthopper eggs to obtain a first transparent container containing rice seedlings parasitized by the parasitic wasp; The rice seedlings parasitized by parasitic wasps in the first transparent container containing the rice seedlings parasitized by parasitic wasps are transferred to a degradable material container with an upper opening, covered with the funnel-shaped lid, and transported to a field to be controlled.

10. The method according to claim 9, characterized in that Before the rice planthopper egg-stage parasitic wasp is hatched, the method further comprises: Putting the opaque container with an upper opening on the outside of the first transparent container containing the parasitic wasp parasitized rice seedlings, covering it with the opaque cover, and inserting the opening of the second transparent container with an upper opening downward into the through hole of the opaque cover to obtain a breeding container containing the parasitic wasp parasitized rice seedlings; culturing the breeding container containing the parasitic wasp parasitized rice seedlings; The culture temperature is 24.0-26.0° C., the relative humidity is 70-90%, and the photoperiod is 12D:12L.

Images