Diglyceride oil composition for reducing lactose, product thereof and preparation method thereof

Abstract

The present invention provides a diglyceride oil composition for reducing lactose, a product thereof, and a preparation method thereof, and relates to the technical field of functional oil processing. The preparation method of the diglyceride oil composition comprises the following steps: (1) mixing corn oil and high-oleic peanut oil, adding glycerol and water, and then adding Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and reacting to obtain material A; (2) mixing seabuckthorn seed oil, Acer truncatum seed oil, and rapeseed oil, adding glycerol and water, and then adding Candida antarctica lipase B and Rhizopus oryzae lipase, and reacting to obtain material B; (3) mixing material A and material B, heating and centrifuging, taking an upper oil sample, and distilling under vacuum conditions to obtain a diglyceride oil composition. The diglyceride oil composition prepared by the present invention has good efficacy in reducing lactose.

Description

Technical Field

[0001] The invention relates to the technical field of functional oil processing, and in particular to a diglyceride oil composition for reducing lactoferrin, a product thereof and a preparation method thereof. Background Art

[0002] Diaceryl is a type of structural lipid in which a fatty acid in a triglyceride is replaced by a hydroxyl group. It is a trace component of natural plant oils and fats and an endogenous intermediate product of fat metabolism in the body. Diaceryl oil exists in plant oils and is mainly composed of diglyceryl. It has the effects of reducing visceral fat, inhibiting weight gain, and lowering blood lipids.

[0003] Visceral fat, also known as internal fat, is a type of fat that exists in the abdominal cavity and surrounds the internal organs. A certain amount of visceral fat is essential for the human body and plays a role in supporting, stabilizing, and protecting the internal organs. However, excessive visceral fat can lead to problems such as infertility, heart disease, and shortness of breath. Existing technologies such as "Research Progress on Physiological Functions and Preparation Technology of Diaceryl Diglycerides" (Wang Yuehua, Cheng Fangyuan, Yang Xinyue, et al., Grain and Food Industry, 2024, 31(2):33-36) record the physiological functions of diglyceryl diglycerides, including but not limited to inhibiting elevated blood lipids, inhibiting fat accumulation, and inhibiting elevated blood sugar, and also record the preparation method of diglyceryl diglycerides. In addition, "Research Progress on Preparation and Application of Diaceryl Diglycerides" (Ren Biwen, Wang Lin, Guangdong Chemical Industry, 2023, 50(14):97-98) also records that diglyceryl diglycerides can reduce visceral fat and also records the preparation method of diglyceryl diglycerides.

[0004] Current methods for preparing diglycerides mainly include enzymatic hydrolysis and chemical methods. The former produces fewer byproducts and produces diglycerides of high purity, while the latter offers lower production costs but higher additional costs and can lead to degradation of heat-resistant polyunsaturated fatty acids. Currently, enzymatic hydrolysis is the most common method for preparing diglycerides. Prior art, such as Chinese Patent CN115678676A, discloses a flavored diglyceride oil and its preparation method, comprising the following steps: mixing crude oil, an enzyme preparation, and a chelating agent, adjusting the moisture content, subjecting the mixture to high-speed shearing, placing the mixture in a shaker for insulation, and filtering to obtain a degummed solution; adding glycerol and lipase to the degummed solution, placing the solution in a vacuum reactor for reaction, collecting the reaction gases using a gas adsorbent during the reaction, and centrifuging the oil layer to obtain a reaction product; molecularly distilling the reaction product to obtain a heavy phase consisting of a mixture of diglycerides and triglycerides, which is fractionated to obtain diglyceride oil; and passing the collected reaction gases through the diglyceride oil for decomposition to obtain the flavored diglyceride oil. The enzymatic degumming method in this invention achieves a moisture content of less than 0.2%, without causing loss of flavor compounds, and retaining over 90% of the oil's flavor. However, the preparation method in this invention focuses on retaining flavor compounds in the oil, aiming to enhance the flavor of diglyceride oil, without further research into the efficacy of diglyceride oil.

[0005] In view of the problems existing in the prior art, it is very necessary to find a diglyceride oil composition for reducing lactose, a product thereof, and a preparation method thereof. Summary of the Invention

[0006] The present invention addresses the problems existing in the prior art and provides a diglyceride oil composition for reducing lactose, a product thereof, and a preparation method thereof. The diglyceride oil of the present invention has excellent lactose-reducing effect.

[0007] The present invention provides a method for preparing a diglyceride oil composition for reducing lactoferrin, comprising the following steps:

[0008] (1) corn oil and high oleic peanut oil are mixed, glycerol and water are added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase are added, and reacted to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 0.1%-12% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 6-8:1;

[0009] (2) After sea buckthorn seed oil, maple seed oil and rapeseed oil are mixed, glycerol and water are added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase are added, and reacted to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase is 0.1%-8% of the total weight of the sea buckthorn seed oil, maple seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase is 0.5-3:1;

[0010] (3) Mix material A and material B, heat and centrifuge, take the upper oil sample, and distill under vacuum conditions to obtain a diglyceride oil composition.

[0011] The molecular free paths of diglycerides and triglycerides are very similar, making it difficult to completely separate them using direct molecular distillation, resulting in a low diglyceride content in the product. The present invention selects appropriate enzymes for enzymatic hydrolysis based on different raw material combinations, allowing for highly selective diglyceride production and increasing the diglyceride content in the product.

[0012] Furthermore, the mass ratio of the seabuckthorn seed oil, the maple seed oil, the rapeseed oil, the corn oil and the high oleic peanut oil is 0.1-20:0.1-20:50-150:10-80:5-20.

[0013] Preferably, the mass ratio of the seabuckthorn seed oil, the maple seed oil, the rapeseed oil, the corn oil and the high oleic peanut oil is 0.1-10:0.1-1:50-100:10-50:5-10.

[0014] Furthermore, the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase in step (1) is 7:1, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase in step (2) is 1:1.

[0015] Furthermore, the total weight of the Rhizomucor miehei lipase and the Pseudomonas cepacia lipase in step (1) is 5%-12% of the total weight of the corn oil and high oleic peanut oil, and the total weight of the Candida antarctica lipase B and the Rhizopus oryzae lipase in step (2) is 3%-8% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil and rapeseed oil.

[0016] Furthermore, the total weight of the Rhizomucor miehei lipase and the Pseudomonas cepacia lipase in step (1) is 10% of the total weight of the corn oil and high oleic peanut oil, and the total weight of the Candida antarctica lipase B and the Rhizopus oryzae lipase in step (2) is 5% of the total weight of the sea buckthorn seed oil, the Acer truncatum seed oil and the rapeseed oil.

[0017] Furthermore, the reaction temperature in steps (1) and (2) is 40-50°C, and the reaction time is 1-5 hours; the heating centrifugation temperature in step (3) is 80-110°C, and the time is 1.5-6 hours.

[0018] Preferably, the reaction temperature in steps (1) and (2) is 42°C, and the reaction time is 2-2.5 hours; the heating centrifugation temperature in step (3) is 90-100°C, and the time is 2-3 hours.

[0019] Furthermore, the reaction temperature in step (1) is 42°C, and the reaction time is 2 hours; the reaction temperature in step (2) is 42°C, and the reaction time is 2.5 hours; and the heating centrifugation temperature in step (3) is 95°C, and the reaction time is 2 hours.

[0020] Furthermore, the weight of the glycerol in step (1) is 40%-45% of the total weight of the corn oil and high oleic peanut oil, and the weight of the glycerol in step (2) is 30%-32% of the total weight of the sea buckthorn seed oil, maple seed oil and rapeseed oil.

[0021] Preferably, the weight of the glycerol in step (1) is 44% of the total weight of the corn oil and high oleic peanut oil, and the weight of the glycerol in step (2) is 32% of the total weight of the sea buckthorn seed oil, maple seed oil and rapeseed oil.

[0022] Preferably, in step (3), after distillation, the method further comprises the steps of adding mulberry juice and Poria cocos extract, and stirring while keeping warm until dissolved.

[0023] Further preferably, the mass ratio of the mulberry juice, the Poria cocos extract and the diglyceride oil composition obtained in step (3) is 0.1-5:0.01-0.15:100, and most preferably is 1:0.05:100.

[0024] Furthermore, the present invention also provides a diglyceride oil composition prepared by the above preparation method.

[0025] Furthermore, the present invention also provides a product for reducing lactose, comprising the diglyceride oil composition prepared by the above preparation method.

[0026] Furthermore, the diglyceride oil composition prepared by the above-mentioned preparation method of the present invention is used to prepare a product with reduced lactose.

[0027] To achieve the above object, the technical solution adopted by the present invention is as follows:

[0028] The technical effects achieved by the present invention are:

[0029] 1. The present invention optimizes the preparation method of the diglyceride oil composition, combines specific diglyceride oil source components, combines corn oil and high oleic peanut oil with Rhizomucor miehei lipase and Pseudomonas cepacia lipase, and combines seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil with Candida antarctica lipase B and Rhizopus oryzae lipase. The resulting diglyceride oil has an excellent fat-reducing effect.

[0030] 2. The preparation method of the present invention specifically adopts the glycerol decomposition process in the enzymatic hydrolysis method to prepare the diglyceride oil composition. Compared with the esterification method and the hydrolysis method, the preparation method of the present invention is simple, efficient, and has lower cost and fewer by-products. While retaining the flavor of sea buckthorn seed oil, maple seed oil, rapeseed oil, corn oil and high oleic acid peanut oil, it has an excellent effect on reducing fat.

[0031] 3. The diglyceride oil obtained by the specific extraction process of the present invention can be further added with mulberry juice and Poria cocos extract to further improve the effect of reducing fat in the body. DETAILED DESCRIPTION

[0032] The following describes the embodiments of the present invention through specific examples. Those skilled in the art will readily understand the other advantages and benefits of the present invention from the disclosure herein. The present invention may also be implemented or applied through various other specific embodiments, and the details in this specification may be modified or altered based on different viewpoints and applications without departing from the spirit of the present invention.

[0033] Before further describing the specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terms used in the examples of the present invention are for describing specific embodiments rather than for limiting the scope of protection of the present invention.

[0034] When numerical ranges are given in the examples, it should be understood that, unless otherwise specified herein, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those of ordinary skill in the art to which the present invention belongs.

[0035] It is worth noting that the transesterification activity of the Rhizomucor miehei lipase used in the present invention is 250 IUN / g; the enzyme activity of the Rhizopus oryzae lipase is 10,000 U / g; the enzyme activity of the Pseudomonas cepacia lipase is 30,000 U / g, and the enzyme activity of the Candida antarctica lipase B is 10,000 U / g.

[0036] The sea buckthorn seed oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd. with the item number 08T20B48801; the Acer truncatum seed oil was purchased from Heze Zhonghe Jianyuan Biotechnology Co., Ltd. with the item number 22T20B48801; the rapeseed oil was purchased from Chengdu Xinxing Grain and Oil Co., Ltd.; the corn oil was purchased from Shandong Yuhuang Grain and Oil Food Co., Ltd. with the item number PY240916107; the high oleic peanut oil was purchased from Shandong Jinsheng Grain and Oil Food Co., Ltd. with the item number GY2405; the mulberry juice was obtained by squeezing and filtering fresh black mulberries, and the Poria cocos extract was purchased from Xinyanghe with the item number XYH-FL-001.

[0037] Example 1

[0038] (1) According to parts by weight, 40 parts of corn oil and 10 parts of high oleic peanut oil were mixed, 22 parts of glycerol and 5 parts of water were added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the mixture was reacted at 42°C for 2 hours to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 10% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to Pseudomonas cepacia lipase was 7:1;

[0039] (2) According to parts by weight, 8 parts of seabuckthorn seed oil, 0.5 parts of Acer truncatum seed oil and 91.5 parts of rapeseed oil were mixed, 32 parts of glycerin and 10 parts of water were added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase were added, and the mixture was reacted at 42°C for 2.5 hours to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase was 5% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase was 1:1;

[0040] (3) After mixing material A and material B, heat and centrifuge at 95°C for 2 hours. Take the upper oil sample and perform molecular distillation at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain a diglyceride oil composition.

[0041] Example 2

[0042] (1) According to parts by weight, 40 parts of corn oil and 10 parts of high oleic peanut oil were mixed, 20 parts of glycerol and 5 parts of water were added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the mixture was reacted at 40°C for 5 hours to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 0.1% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to Pseudomonas cepacia lipase was 6:1;

[0043] (2) According to parts by weight, 8 parts of seabuckthorn seed oil, 0.5 parts of Acer truncatum seed oil and 91.5 parts of rapeseed oil were mixed, 30 parts of glycerin and 10 parts of water were added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase were added, and the mixture was reacted at 40°C for 5 hours to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase was 0.1% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase was 0.5:1;

[0044] (3) After mixing material A and material B, heat and centrifuge at 80°C for 6 hours. Take the upper oil sample and perform molecular distillation at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain a diglyceride oil composition.

[0045] Example 3

[0046] (1) According to parts by weight, 40 parts of corn oil and 10 parts of high oleic peanut oil were mixed, 22.5 parts of glycerol and 10 parts of water were added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the mixture was reacted at 50° C. for 1 hour to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 12% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase was 8:1;

[0047] (2) According to parts by weight, 8 parts of seabuckthorn seed oil, 0.5 parts of Acer truncatum seed oil and 91.5 parts of rapeseed oil were mixed, 30 parts of glycerin and 10 parts of water were added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase were added, and the mixture was reacted at 50°C for 1 hour to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase was 8% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase was 3:1;

[0048] (3) After mixing material A and material B, heat and centrifuge at 110°C for 1.5 hours. Take the upper oil sample and perform molecular distillation at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain a diglyceride oil composition.

[0049] Example 4

[0050] (1) According to parts by weight, 40 parts of corn oil and 10 parts of high oleic peanut oil were mixed, 22 parts of glycerol and 5 parts of water were added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the mixture was reacted at 42°C for 2 hours to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 5% of the total weight of the corn oil and peanut oil, and the weight ratio of the Rhizomucor miehei lipase to Pseudomonas cepacia lipase was 7:1;

[0051] (2) According to parts by weight, 8 parts of seabuckthorn seed oil, 0.5 parts of Acer truncatum seed oil and 91.5 parts of rapeseed oil were mixed, 32 parts of glycerin and 10 parts of water were added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase were added, and the mixture was reacted at 42°C for 2.5 hours to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase was 3% of the total weight of the seabuckthorn seed oil, Acer truncatum seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase was 1:1;

[0052] (3) After mixing material A and material B, heat and centrifuge at 95°C for 2 hours. Take the upper oil sample and perform molecular distillation at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain a diglyceride oil composition.

[0053] Example 5

[0054] The diglyceride oil composition prepared in Example 1 was mixed with mulberry juice and Poria cocos extract in a mass ratio of 100:1:0.05, and stirred at 80° C. until dissolved to obtain a composition.

[0055] Comparative Example 1

[0056] The difference from Example 1 is that in step (1), the weight ratio of Rhizomucor miehei lipase to Pseudomonas cepacia lipase is 1:1, and the rest are the same.

[0057] Comparative Example 2

[0058] The difference from Example 1 is that in step (1), only Rhizomucor miehei lipase is used, and the rest are the same.

[0059] Comparative Example 3

[0060] The difference from Example 1 is that in step (2), the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase is 10:1, and the rest are the same.

[0061] Comparative Example 4

[0062] The difference from Example 1 is that in step (2), only Candida antarctica lipase B is used, and the rest are the same.

[0063] Comparative Example 5

[0064] (1) According to parts by weight, 40 parts of corn oil, 10 parts of high oleic peanut oil, 8 parts of sea buckthorn seed oil, 0.5 parts of maple seed oil and 91.5 parts of rapeseed oil were mixed, 54 parts of glycerin and 15 parts of water were added, and Rhizomucor miehei lipase and Pseudomonas cepacia lipase were added, and the mixture was reacted at 42°C for 4.5 hours to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 10% of the total weight of the corn oil, high oleic peanut oil, sea buckthorn seed oil, maple seed oil and rapeseed oil, and the weight ratio of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase was 7:1;

[0065] (2) Material A was heated and centrifuged at 95°C for 2 h, and the upper oil sample was taken for molecular distillation at an evaporation surface temperature of 120°C, a feed rate of 10 kg / min, a scraper speed of 200 r / min, and a vacuum degree of 0.1 Pa to obtain a diglyceride oil composition.

[0066] Comparative Example 6

[0067] The difference from Example 1 is that 50 parts of whole corn oil are used in step (1), and 100 parts of whole rapeseed oil are used in step (2), and the rest are the same.

[0068] Comparative Example 7

[0069] The diglyceride oil composition prepared in Example 1 was mixed with mulberry juice and Poria cocos extract in a mass ratio of 100:0.05:1, and stirred at 80° C. until dissolved to obtain a diglyceride oil composition.

[0070] Result detection

[0071] 1. Diaceride content detection

[0072] The diglyceride content was determined according to GB / T 26636, and the upper layer oil sample before molecular distillation and the diglyceride oil composition obtained after molecular distillation were tested respectively.

[0073] The test results are shown in Table 1:

[0074] Table 1

[0075]

[0076] The results show that the diglyceride content in the diglyceride oil compositions prepared in Examples 1-4 was significantly increased. In step (1), it was essential to select an appropriate ratio of Rhizomucor miehei lipase and Pseudomonas cepacia lipase. The results of Comparative Examples 1 and 2 indicate that the addition of a single enzyme or an inappropriate combination ratio significantly reduced the diglyceride content. Similarly, in step (2), it was essential to select an appropriate ratio of Candida antarctica lipase B and Rhizopus oryzae lipase. The results of Comparative Examples 3 and 4 indicate that the addition of a single enzyme or an inappropriate combination ratio significantly reduced the diglyceride content. The appropriate enzyme addition amount has a significant effect on the composition of the enzymatic hydrolysis product. Comparative Example 5 attempted a one-step enzymatic hydrolysis, and the diglyceride content was extremely low. Comparative Example 6 used only corn oil and rapeseed oil as raw materials, and the results were also significantly different from those of Example 1.

[0077] 2. Internal fat reduction experiment

[0078] Experimental Animals: C57BL6J male mice weighing 20-22g were fed a high-fat diet with free access to water and food. After 4 months, an obesity model was established. The obese mice were randomly divided into a model group, Example 1 group, Example 5 group, Comparative Example 5 group, and Comparative Example 7 group. C57BL6J male mice fed a normal diet served as a blank group. Each group had 10 mice.

[0079] Experimental method: The model group, Example 1 group, Example 5 group, Comparative Example 5 group, and Comparative Example 7 group were continued to be fed a high-fat diet. At the same time, the Example 1 group, Example 5 group, Comparative Example 5 group, and Comparative Example 7 group were gavage-administered once a day at a dose of 0.05 mL / 10 g, and the diglyceride oil compositions of Example 1, Example 5, Comparative Example 5, and Comparative Example 7 were administered, respectively. The blank group and the model group were gavaged with an equal amount of normal saline every day. The body weight of the mice was measured on the 15th, 30th, and 60th day of administration. The mice were sacrificed and the liver weight and intra-abdominal fat weight were measured.

[0080] The results of mouse body weight determination are shown in Table 2:

[0081] Table 2

[0082]

[0083] Note: Compared with the model group and the blank group, **P<0.01; compared with the experimental group (Example 1 group, Example 5 group, Comparative Example 5 group, Comparative Example 7 group) and the model group, ## P < 0.01; # P<0.05.

[0084] The results of mouse liver weight and intra-abdominal fat weight are shown in Table 3:

[0085] Table 3

[0086]

[0087] Note: Compared with the model group and the blank group, **P<0.01; compared with the experimental group (Example 1 group, Example 5 group, Comparative Example 5 group, Comparative Example 7 group) and the model group, ## P < 0.01; # P<0.05.

[0088] During the experiment, food intake did not differ significantly within or between experimental groups. However, significant differences were observed in body weight, liver weight, and intra-abdominal fat weight. The diglyceride oil compositions of Examples 1 and 5 were more effective than Comparative Example 5 in reducing body weight and intra-abdominal fat. Example 5 was superior to Comparative Example 7, so the combination of the diglyceride oil composition, mulberry juice, and Poria cocos extract in Example 5 was selected.

[0089] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A method for preparing a diglyceride oil composition for reducing lactose, characterized in that: The following steps are involved: (1) corn oil and high oleic peanut oil are mixed, glycerol and water are added, and then Rhizomucor miehei lipase and Pseudomonas cepacia lipase are added, and reacted to obtain material A; the total weight of the Rhizomucor miehei lipase and Pseudomonas cepacia lipase is 0.1%-12% of the total weight of the corn oil and high oleic peanut oil, and the weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase is 6-8:1; (2) After sea buckthorn seed oil, maple seed oil and rapeseed oil are mixed, glycerol and water are added, and then Antarctic Candida lipase B and Rhizopus oryzae lipase are added, and reacted to obtain material B; the total weight of the Antarctic Candida lipase B and Rhizopus oryzae lipase is 0.1%-8% of the total weight of the sea buckthorn seed oil, maple seed oil and rapeseed oil, and the weight ratio of the Antarctic Candida lipase B to Rhizopus oryzae lipase is 0.5-3:1; (3) Mix material A and material B, heat and centrifuge, take the upper oil sample, and distill under vacuum conditions to obtain a diglyceride oil composition.

2. The preparation method according to claim 1, characterized in that The mass ratio of the seabuckthorn seed oil, the maple seed oil, the rapeseed oil, the corn oil and the high-oleic peanut oil is 0.1-20:0.1-20:50-150:10-80:5-20.

3. The preparation method according to claim 1, characterized in that The weight ratio of the Rhizomucor miehei lipase to the Pseudomonas cepacia lipase in step (1) is 7:1, and the weight ratio of the Candida antarctica lipase B to the Rhizopus oryzae lipase in step (2) is 1:

1.

4. The preparation method according to claim 1, characterized in that The total weight of the Rhizomucor miehei lipase and the Pseudomonas cepacia lipase in step (1) is 10% of the total weight of the corn oil and high oleic peanut oil, and the total weight of the Antarctic Candida lipase B and the Rhizopus oryzae lipase in step (2) is 5% of the total weight of the sea buckthorn seed oil, Acer truncatum seed oil and rapeseed oil.

5. The preparation method according to claim 1, characterized in that The reaction temperature in steps (1) and (2) is 40-50°C, and the reaction time is 1-5 hours; the heating centrifugation temperature in step (3) is 80-110°C, and the reaction time is 1.5-6 hours.

6. The preparation method according to claim 1, characterized in that The weight of the glycerol in step (1) is 40%-45% of the total weight of the corn oil and high oleic peanut oil, and the weight of the glycerol in step (2) is 30%-32% of the total weight of the sea buckthorn seed oil, maple seed oil and rapeseed oil.

7. The preparation method according to claim 1, characterized in that In step (3), after distillation, the method further comprises the steps of adding mulberry juice and Poria extract, and stirring while keeping warm until dissolved.

8. The preparation method according to claim 7, characterized in that The mass ratio of the mulberry juice, the Poria cocos extract and the diglyceride oil composition is 0.1-5:0.01-0.15:

100.

9. A diglyceride oil composition obtained according to the preparation method according to any one of claims 1 to 8.

10. A product for reducing internal fat, characterized in that: The invention relates to a diglyceride oil composition prepared by the preparation method according to any one of claims 1 to 8.

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