Plant-derived polypeptide and cosmetic containing same

Abstract

The invention discloses a botanical polypeptide and a cosmetic containing the botanical polypeptide, and relates to the technical field of cosmetics. The preparation method comprises the following steps: firstly, carrying out enzymolysis on mung bean protein to obtain small molecule peptide consisting of three to six amino acids, and forming a plant polypeptide metal chelate with stable chemical properties with copper ions; the metal chelate can improve permeability, so that main components of small molecule peptide can enter the interior of skin, mitochondrial activity is stimulated, inflammation is reduced, meanwhile, anaphylactic reaction is not caused, then chitosan and sodium alginate free radicals are polymerized, calcium ion crosslinking is assisted, hydrogel balls of a polymer network structure are formed, and the hydrogel balls are used for preparing the hydrogel. Lecithin is stably loaded on the hydrogel balls; in the use process, the embedded plant polypeptide metal chelate is released and acts on the skin, and meanwhile lecithin is adsorbed on the surface of the skin to form a grease protection layer. The cosmetic prepared by the invention has antibacterial and protective effects.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and specifically to a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide. Background Art

[0002] A makeup primer is a type of cosmetic. In the conventional makeup process, generally, a makeup primer is used first, and then other makeup products are applied successively. That is to say, the makeup primer is the first makeup process in which makeup comes into contact with the human facial skin. In this process, the makeup primer can establish a barrier between the skin and the makeup, reducing the situation where powder particles in the makeup enter the pores and texture. However, after the cosmetic is opened and stored at room temperature, it is easy to breed bacteria and affect the health of the skin. Covering the face for a long time is also likely to cause skin inflammation.

[0003] Most of the existing makeup primers on the market are a type of whitening cosmetic. It is mainly used to modify the disadvantages of uneven skin color and dullness. Local use can make the skin look perfect, but it lacks the performance of keeping the skin healthy. In order to prevent the impact of subsequent makeup, while having antibacterial effects, the makeup primer should also form an oily film with a protective effect to reduce the damage of cosmetics to the skin. However, there are almost no makeup primers that can balance protection and antibacterial effects in the current market. The present invention will provide a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide, namely a makeup primer with antibacterial and protective effects, to meet the market demand. Summary of the Invention

[0004] The purpose of the present invention is to provide a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide to solve the problems existing in the prior art.

[0005] To solve the above technical problems, the present invention provides the following technical solution: A plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide, wherein the cosmetic is a makeup primer prepared by using composite hydrogel spheres encapsulating plant polypeptide metal chelates and simultaneously loading lecithin as effective factors.

[0006] Further, the plant polypeptide metal chelate is a compound formed by chelating small peptides enzymatically hydrolyzed from mung bean with copper ions.

[0007] Further, the composite hydrogel sphere is formed by free radical polymerization and cross-linking of chitosan and sodium alginate with calcium ions to encapsulate the plant polypeptide metal chelate.

[0008] Further, a preparation method of a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide includes the following preparation steps: (1) Dissolve 40 g of mung bean protein in 1 L of deionized water. First, pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution. Add 5 mL of alkaline protease, and maintain the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme in a boiling water bath at 100 °C for 1 h to obtain a hydrolysate. Cool the hydrolysate to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, and then centrifuge at 5000 rpm for 20 min to collect the supernatant. Filter the supernatant through an ultrafiltration membrane under a pressure of 0.25 MPa to obtain a filtrate of small molecular peptides of mung bean protein. Freeze-dry the filtrate of small molecular peptides of mung bean protein for later use. Dissolve 50 g of small molecular peptides of mung bean protein in 1 L of deionized water, adjust the pH to 6 with 1 mol / L hydrochloric acid aqueous solution, add 1 - 9 mg of copper sulfate hydrate, and stir at a speed of 200 rpm for chelation reaction. The reaction temperature is 50 °C, and the reaction time is 60 - 100 min. After the reaction, cool to 25 °C, precipitate with 3 L of absolute ethanol for 2 - 5 h, centrifuge at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol. First, set the oven temperature to 65 °C and bake for 1 h, then reduce the temperature to 30 °C and bake the chelate for 3 h to obtain a plant polypeptide metal chelate. (2) Dissolve 10 - 20 g of chitosan in 1 L of deionized water, dropwise add 20 - 200 g of methacrylic anhydride, and adjust the pH to 8 - 10 with an alkaline solution, then stir at 60 rpm for 20 min to obtain a chitosan aqueous solution. Dissolve 10 - 20 g of sodium alginate in 1 L of deionized water, dropwise add 20 - 200 g of methacrylic anhydride, and adjust the pH to 8 - 10 with an alkaline solution, then stir at 60 rpm for 20 min to obtain a sodium alginate aqueous solution. Mix the chitosan aqueous solution and the sodium alginate aqueous solution with a mass ratio of 1:10 - 10:1 to obtain a mixed solution. First, add 0.5 - 1.5% of the plant polypeptide metal chelate based on the total mass of the mixed solution, stir at 60 rpm for 20 min, then add 5 - 15% of calcium chloride based on the mass of sodium alginate, mix and stir at 60 rpm for 30 min. Add a photoinitiator to the solution and irradiate it under a 100 W ultraviolet lamp for 2 - 10 min to obtain hydrogel beads. Put 50 g of hydrogel beads into 1 L of deionized water, add 2 - 5 g of lecithin, and stir slowly at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate, and wash it 3 times with deionized water to prepare composite hydrogel beads. (3) Add appropriate amount of deionized water, 2 - 6 g of glycerol, 5 - 9 g of butanediol, and 2 - 3 g of ethanol to the water pot, heat up to 85 °C, maintain for 10 min, cool down to 65 °C, add 1 - 3 g of 1,2 - pentanediol, stir at 60 rpm for 5 min to prepare the water pot material; then add 2 - 5 g of cyclopentasiloxane, 2 - 3 g of PEG - 10 dimethicone, 3 - 5 g of trimethylsiloxysilicate, 6 - 8 g of phenyltrimethylsiloxane, 3 - 5 g of lauryl PEG - 9 dimethicone / dimethicone copolymer, 2 - 6 g of glyceryl tri(ethylhexanoate), 3 - 5 g of octyl polymethylsiloxane, 1 - 3 g of polyglyceryl - 2 isostearate, and 1 - 3 g of composite hydrogel beads into the reaction pot, homogenize and disperse at 20 rpm for 20 min to obtain the reaction pot material; heat up to 65 °C, slowly add the water pot material into the reaction pot in 3 portions, stir at 20 rpm for 10 min; when stirring and cooling down to 45 °C, add 0.1 - 1 g of phenoxyethanol, stir at 20 rpm for another 10 min, and cool down to 25 °C to obtain the antibacterial and protective makeup primer.

[0009] Further, in the step (1), the ultrafiltration membrane is composed of ultrafiltration membranes with a molecular weight cut - off of 5 ku and 3 ku.

[0010] Further, in the step (1), the copper sulfate hydrate is CuSO4·5H2O.

[0011] Further, in the step (2), the alkaline solution is a 0.5 mol / L aqueous sodium hydroxide solution.

[0012] Further, in the step (2), the photoinitiator is I2959, and the addition amount of the photoinitiator is 0.05 - 0.25% of the volume of the mixed solution.

[0013] Further, in the step (2), the ultraviolet intensity is 3600 - 6000 μW / cm.

[0014] Further, in the step (3), the addition amount of deionized water is 30 - 40 g.

[0015] Compared with the prior art, the beneficial effects achieved by the present invention are: The present invention prepares composite hydrogel beads that embed plant polypeptide metal chelates and simultaneously load lecithin. Using such composite hydrogel beads as an effective factor and adding them into the makeup primer to prepare a makeup primer with antibacterial and protective effects.

[0016] First, mung bean protein is enzymatically hydrolyzed to generate small peptides composed of three to six amino acids. Through the action of coordination covalent chemical bonds, a chemically stable complex is formed between copper ions and mung bean small peptides. Under appropriate conditions, groups such as amino groups on the main component arginine of the small peptide can act as ligands to provide electrons for metal copper ions, forming a stable plant polypeptide metal chelate. This metal chelate can act on facial skin, improve its permeability, enhance the penetration so that arginine can enter the skin interior, effectively stimulate mitochondrial activity, accelerate cell metabolism, thereby improving the cell's resistance to external stimuli and reducing inflammation. Moreover, metal copper ions themselves have a certain antibacterial effect. The combination of the two is synergistic, making the skin after using the primer less likely to be infected by bacteria during subsequent makeup application. Mung bean polypeptide, as a plant extract, has low antigenicity and will not cause allergic reactions even when directly acting on the skin interior.

[0017] Secondly, using natural polymer chitosan and natural extract sodium alginate as the matrix, a hydrogel encapsulating the chelate is prepared by free radical polymerization, and calcium ion cross-linking is supplemented to form hydrogel beads with a polymer network structure built by two molecular chains, making the hydrogel beads have good acid-base stability. This kind of hydrogel beads has a porous and rough surface structure that provides binding sites. The negatively charged lecithin electrostatically binds with the positively charged calcium ions, making lecithin stably loaded on the hydrogel beads. During use, the hydrogel beads are rubbed and squeezed, releasing the encapsulated plant polypeptide metal chelate, which acts on the skin and at the same time adsorbs lecithin to form a thin oil protection layer on the skin surface, making the subsequent makeup products of the primer less likely to have a negative irritating impact on the skin, thus achieving a protective effect. Specific embodiments

[0018] The following will combine the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

[0019] To more clearly illustrate the method provided by the present invention, the following examples are used for detailed description. The test methods for each index of a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide prepared in the following examples are as follows: Antibacterial property: The 24-hour slant cultures of the test bacteria (Staphylococcus aureus, Streptococcus, Escherichia coli) are washed down with PBS to make a bacterial suspension (the required concentration is: when 100 μL is dropped on the control sample piece or in 5 mL of the sample solution, the recovered number of bacteria is 1 104 -9 104cfu / ml). Take 5 ml each of the sample aqueous solutions with a concentration of 10% prepared from the samples of the examples and comparative examples and the reference substance (deionized water), with 4 tubes for each. Take the above-mentioned bacterial suspension and add 100 μL to each sample and reference substance, and mix evenly. Start timing. After acting for 1 day, use sterile forceps to take the sample (0.5 mL) and put it into a test tube containing 5 mL of PBS, mix well, make appropriate dilutions, then take 2-3 dilution degrees among them, respectively pipette 0.5 mL, place it in two petri dishes, pour 15 mL of nutrient agar medium cooled to 40 - 45 °C, rotate the petri dishes to make it fully uniform. After the agar solidifies, turn the plate over and culture at 35 °C ± 2 °C for 48 h, and perform viable bacterial colony counting. Calculate the antibacterial rate: X = (A - B) / A × 100%.

[0020] Protective property: The makeup primers prepared from the examples and comparative examples were respectively given to 30 testers for comparative use. Apply 0.5 ml of the makeup primer evenly on the left face for 20 min, and apply 0.5 ml of the moisturizing lotion without any functional factors evenly on the right face for 20 min. Then apply 1 ml of the same brand of liquid foundation evenly on the whole face. After 8 h, the testers make evaluations according to their own feelings. The trial contents include: whether there is a sense of irritation, whether the facial skin is red, and whether the skin around the eyes is red when contacted; the evaluation criteria are "yes", "slight", and "no".

[0021] Example 1 (1) Dissolve 40 g of mung bean protein in 1 L of deionized water, first pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, add 5 mL of alkaline protease, and keep the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme in a boiling water bath at 100 °C for 1 h to obtain a hydrolyzate; cool the hydrolyzate to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, then centrifuge at 5000 rpm for 20 min, and take the supernatant; filter the supernatant through an ultrafiltration membrane under a pressure of 0.25 MPa. The ultrafiltration membrane consists of ultrafiltration membranes with a molecular weight cut-off of 5 ku and 3 ku to obtain a filtrate of small molecular peptides of mung bean protein; freeze-dry the filtrate of small molecular peptides of mung bean protein for later use; dissolve 50 g of small molecular peptides of mung bean protein in 1 L of deionized water, adjust the pH to 6 with 1 mol / L hydrochloric acid aqueous solution, add 1 mg of CuSO4·5H2O, stir at a speed of 200 rpm for chelation reaction, the reaction temperature is 50 °C, the reaction time is 60 min. After the reaction ends, cool to 25 °C, precipitate with 3 L of absolute ethanol for 2 h, centrifuge at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol; first adjust the temperature of the oven to 65 °C and bake for 1 h, then reduce the temperature to 30 °C and bake the chelate for 3 h to obtain a plant polypeptide metal chelate; (2) Dissolve 10 g of chitosan in 1 L of deionized water, add 20 g of methacrylic anhydride dropwise, and adjust the pH to 8 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous chitosan solution; dissolve 10 g of sodium alginate in 1 L of deionized water, add 20 g of methacrylic anhydride dropwise, and adjust the pH to 8 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous sodium alginate solution; mix the aqueous chitosan solution and the aqueous sodium alginate solution with a mass ratio of 1:10 to obtain a mixed solution. First, add 0.5% of the total mass of the plant polypeptide metal chelate based on the mixed solution, stir at 60 rpm for 20 min, then add 5% of the mass of calcium chloride based on the mass of sodium alginate, mix and stir at 60 rpm for 30 min. Add the photoinitiator I2959 to the solution, and the addition amount of the photoinitiator is 0.05% of the volume of the mixed solution. Irradiate under a 100 W ultraviolet lamp for 2 min, and the ultraviolet intensity is 3600 μW / cm to obtain hydrogel beads; put 50 g of hydrogel beads into 1 L of deionized water, add 2 g of lecithin, and stir slowly at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate and wash it 3 times with deionized water to prepare composite hydrogel beads; (3) Add 30 g of deionized water, 2 g of glycerol, 5 g of butanediol, and 2 g of ethanol to a water pot and heat it to 85 °C, keep it for 10 min, cool it to 65 °C, add 1 g of 1,2-pentanediol, and stir at 60 rpm for 5 min to prepare the water pot material; then add 2 g of cyclopentasiloxane, 2 g of PEG-10 dimethicone, 3 g of trimethylsiloxysilicate, 6 g of phenyltrimethicone, 3 g of lauryl PEG-9 dimethicone dimethicone, 2 g of glyceryl tri(ethylhexanoate), 3 g of octyl polymethylsiloxane, 1 g of polyglyceryl-2 isostearate, and 1 g of composite hydrogel beads to a reaction pot, and homogenize and disperse at 20 rpm for 20 min to prepare the reaction pot material; heat it to 65 °C, and slowly add the water pot material to the reaction pot in 3 portions, and stir at a speed of 20 rpm for 10 min; when stirring and cooling to 45 °C, add 0.1 g of phenoxyethanol, and stir at a speed of 20 rpm for another 10 min, and cool to 25 °C to obtain the antibacterial protective makeup primer.

[0022] Example 2 (1) Dissolve 40 g of mung bean protein in 1 L of deionized water. First, pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, add 5 mL of alkaline protease, and maintain the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme in a boiling water bath at 100 °C for 1 h to obtain a hydrolyzate. Cool the hydrolyzate to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, and then centrifuge at 5000 rpm for 20 min to collect the supernatant. Filter the supernatant through an ultrafiltration membrane under a pressure of 0.25 MPa. The ultrafiltration membrane consists of ultrafiltration membranes with a molecular weight cut-off of 5 ku and 3 ku to obtain a filtrate of small molecular peptides of mung bean protein. Lyophilize the filtrate of small molecular peptides of mung bean protein for later use. Dissolve 50 g of small molecular peptides of mung bean protein in 1 L of deionized water, adjust the pH to 6 with 1 mol / L hydrochloric acid aqueous solution, add 5 mg of CuSO4·5H2O, and stir at a speed of 200 rpm for chelation reaction. The reaction temperature is 50 °C and the reaction time is 80 min. After the reaction, cool to 25 °C, precipitate with 3 L of absolute ethanol for 3.5 h, centrifuge at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol. First, adjust the temperature of the oven to 65 °C and bake for 1 h, then lower the temperature to 30 °C and bake the chelate for 3 h to obtain a plant polypeptide metal chelate. (2) Dissolve 15 g of chitosan in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, and stir at 60 rpm for 20 min to obtain a chitosan aqueous solution. Dissolve 15 g of sodium alginate in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, and stir at 60 rpm for 20 min to obtain a sodium alginate aqueous solution. Mix the chitosan aqueous solution and the sodium alginate aqueous solution with a mass ratio of 1:1 to obtain a mixed solution. First, add 1% of the plant polypeptide metal chelate based on the total mass of the mixed solution, stir at 60 rpm for 20 min, then add 10% of calcium chloride based on the mass of sodium alginate, mix and stir at 60 rpm for 30 min. Add a photoinitiator I2959 to the solution. The addition amount of the photoinitiator is 0.1% of the volume of the mixed solution, and irradiate it under a 100 W ultraviolet lamp for 6 min. The ultraviolet intensity is 4800 μW / cm to obtain hydrogel beads. Put 50 g of hydrogel beads into 1 L of deionized water, add 3.5 g of lecithin, and stir slowly at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate, and wash it 3 times with deionized water to prepare composite hydrogel beads. (3) Add 35 g of deionized water, 4 g of glycerol, 7 g of butanediol, and 2.5 g of ethanol to the water pot, heat it up to 85 °C, maintain for 10 min, cool down to 65 °C, add 2 g of 1,2-pentanediol, and stir at 60 rpm for 5 min to prepare the water pot material; then add 3.5 g of cyclopentasiloxane, 2.5 g of PEG-10 dimethicone, 4 g of trimethylsilyloxy silicate, 7 g of phenyl trimethylsiloxane, 4 g of lauryl PEG-9 dimethicone / dimethicone copolymer, 4 g of glyceryl tri(ethylhexanoate), 4 g of octyl polymethylsiloxane, 2 g of polyglyceryl-2 isostearate, and 2 g of composite hydrogel beads into the reaction pot, homogenize and disperse at 20 rpm for 20 min to obtain the reaction pot material; heat up to 65 °C, and slowly add the water pot material into the reaction pot in 3 portions, and stir at 20 rpm for 10 min; when stirring and cooling down to 45 °C, add 0.55 g of phenoxyethanol, and stir at 20 rpm for another 10 min, then cool down to 25 °C to obtain the antibacterial protective makeup primer.

[0023] Example 3 (1) Dissolve 40 g of mung bean protein in 1 L of deionized water, first pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, add 5 mL of alkaline protease, and maintain the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme in a boiling water bath at 100 °C for 1 h to obtain a hydrolysis solution; cool the hydrolysis solution to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, and then centrifuge at 5000 rpm for 20 min to take the supernatant; filter the supernatant through an ultrafiltration membrane under a pressure of 0.25 MPa. The ultrafiltration membrane consists of ultrafiltration membranes with a molecular weight cut-off of 5 ku and 3 ku to obtain a filtrate of mung bean protein small peptides; freeze-dry the filtrate of mung bean protein small peptides for later use; dissolve 50 g of mung bean protein small peptides in 1 L of deionized water, adjust the pH to 6 with 1 mol / L hydrochloric acid aqueous solution, add 9 mg of CuSO4·5H2O, and stir at 200 rpm for chelation reaction. The reaction temperature is 50 °C, and the reaction time is 100 min. After the reaction is completed, cool it to 25 °C, precipitate with 3 L of absolute ethanol for 5 h, centrifuge at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol; first adjust the temperature of the oven to 65 °C and bake for 1 h, then reduce the temperature to 30 °C and bake the chelate for 3 h to obtain the plant polypeptide metal chelate. (2) Dissolve 20 g of chitosan in 1 L of deionized water, add 200 g of methacrylic anhydride dropwise, and adjust the pH to 10 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous chitosan solution; dissolve 20 g of sodium alginate in 1 L of deionized water, add 200 g of methacrylic anhydride dropwise, and adjust the pH to 10 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous sodium alginate solution; mix the aqueous chitosan solution and the aqueous sodium alginate solution with a mass ratio of 10:1 to obtain a mixed solution. First, add 1.5% of the total mass of the mixed solution of the plant polypeptide metal chelate, stir at 60 rpm for 20 min, then add 15% of the mass of sodium alginate of calcium chloride, mix and stir at 60 rpm for 30 min. Add the photoinitiator I2959 to the solution, and the addition amount of the photoinitiator is 0.25% of the volume of the mixed solution. Irradiate under a 100 W ultraviolet lamp for 10 min, and the ultraviolet intensity is 6000 μW / cm to obtain hydrogel balls; put 50 g of hydrogel balls into 1 L of deionized water, add 5 g of lecithin, and stir slowly at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate, and wash it 3 times with deionized water to prepare composite hydrogel balls; (3) Add 40 g of deionized water, 6 g of glycerol, 9 g of butanediol, and 3 g of ethanol to a water pot and heat it to 85 °C, keep it for 10 min, cool it to 65 °C, add 3 g of 1,2-pentanediol, and stir at 60 rpm for 5 min to prepare the water pot material; then add 5 g of cyclopentasiloxane, 3 g of PEG-10 dimethicone, 5 g of trimethylsiloxysilicate, 8 g of phenyltrimethicone, 5 g of lauryl PEG-9 dimethicone / dimethicone copolymer, 6 g of glyceryl tri(ethylhexanoate), 5 g of octyl polymethylsiloxane, 3 g of polyglyceryl-2 isostearate, and 3 g of composite hydrogel balls to a reaction pot, and homogenize and disperse at 20 rpm for 20 min to prepare the reaction pot material; heat it to 65 °C, and slowly add the water pot material to the reaction pot in 3 portions, and stir at a speed of 20 rpm for 10 min; when stirring and cooling to 45 °C, add 1 g of phenoxyethanol, and stir at a speed of 20 rpm for another 10 min, and cool to 25 °C to obtain the antibacterial protective makeup primer.

[0024] Comparative Example 1 The difference between Comparative Example 1 and Example 2 lies in the difference in step (1). Step (1) is changed to: Dissolve 40 g of mung bean protein in 1 L of deionized water, first pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, add 5 mL of alkaline protease, and maintain the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme through a boiling water bath at 100 °C for 1 h to obtain a hydrolyzate; cool the hydrolyzate to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, then centrifuge at 5000 rpm for 20 min, take the supernatant to obtain a mung bean protein heteropeptide filtrate; freeze-dry the mung bean protein heteropeptide filtrate for later use; dissolve 50 g of mung bean protein heteropeptide in 1 L of deionized water, adjust the pH to 6 with 1 mol / L hydrochloric acid aqueous solution, add 5 mg of CuSO4·5H2O, stir at a speed of 200 rpm for chelation reaction, the reaction temperature is 50 °C, the reaction time is 80 min. After the reaction, cool to 25 °C, precipitate with 3 L of absolute ethanol for 3.5 h, centrifuge at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol; first adjust the temperature of the oven to 65 °C and bake for 1 h, then reduce the temperature to 30 °C and bake the chelate for 3 h to obtain a plant polypeptide metal chelate; the remaining steps are the same as in Example 2.

[0025] Comparative Example 2 The differences between Comparative Example 2 and Example 2 lie in the differences in steps (1) and (2). Steps (1) and (2) are changed to: (1) Dissolve 40 g of mung bean protein in 1 L of deionized water, first pretreat it in a water bath at 90 °C for 20 min. After the temperature drops to 55 °C, adjust the pH to 9 with 0.5 mol / L sodium hydroxide aqueous solution, add 5 mL of alkaline protease, and maintain the pH at 9 with 0.5 mol / L sodium hydroxide aqueous solution. After hydrolysis for 4 h, inactivate the enzyme through a boiling water bath at 100 °C for 1 h to obtain a hydrolyzate; cool the hydrolyzate to 25 °C, adjust the pH value to 7 with 1 mol / L hydrochloric acid aqueous solution, then centrifuge at 5000 rpm for 20 min, take the supernatant; filter the supernatant through an ultrafiltration membrane under a pressure of 0.25 MPa. The ultrafiltration membrane consists of ultrafiltration membranes with a molecular weight cut-off of 5 ku and 3 ku to obtain a mung bean protein small peptide filtrate; centrifuge the mung bean protein small peptide filtrate at 10000 rpm for 15 min, collect the precipitate, and wash it 3 times with absolute ethanol; first adjust the temperature of the oven to 50 °C and bake for 1 h, then reduce the temperature to 30 °C and dry for 3 h to obtain a plant polypeptide; (2) Dissolve 15 g of chitosan in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous chitosan solution; dissolve 15 g of sodium alginate in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain an aqueous sodium alginate solution; mix the aqueous chitosan solution and the aqueous sodium alginate solution with a mass ratio of 1:1 to obtain a mixed solution. First, add 1% of the total mass of the mixed solution of plant polypeptide, stir at 60 rpm for 20 min, then add 10% of the mass of sodium alginate of calcium chloride, mix and stir at 60 rpm for 30 min. Add photoinitiator I2959 to the solution, and the addition amount of the photoinitiator is 0.1% of the volume of the mixed solution. Irradiate under a 100 W ultraviolet lamp for 6 min, and the ultraviolet intensity is 4800 μW / cm to obtain hydrogel beads; put 50 g of hydrogel beads into 1 L of deionized water, add 3.5 g of lecithin, and stir slowly at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate, and wash it 3 times with deionized water to prepare composite hydrogel beads; the remaining steps are the same as in Example 2.

[0026] Comparative Example 3 The difference between Comparative Example 3 and Example 2 is that step (2) is not included. Step (3) is changed to: Add 35 g of deionized water, 4 g of glycerol, 7 g of butanediol, and 2.5 g of ethanol to the water pot and heat up to 85 °C, keep for 10 min, cool down to 65 °C, add 2 g of 1,2-pentanediol, and stir at 60 rpm for 5 min to prepare the water pot material; then add 3.5 g of cyclopentasiloxane, 2.5 g of PEG-10 dimethicone, 4 g of trimethylsiloxysilicate, 7 g of phenyltrimethylsiloxane, 4 g of lauryl PEG-9 dimethicone / dimethicone copolymer, 4 g of glyceryl tri(ethylhexanoate), 4 g of octyl polymethylsiloxane, 2 g of polyglyceryl-2 isostearate, 1.5 g of plant polypeptide, and 0.5 g of lecithin to the reaction pot, and homogenize and disperse at 20 rpm for 20 min to prepare the reaction pot material; heat up to 65 °C, and slowly add the water pot material to the reaction pot in 3 portions, and stir at a speed of 20 rpm for 10 min; when stirring and cooling down to 45 °C, add 0.55 g of phenoxyethanol, and stir at a speed of 20 rpm for another 10 min, and cool down to 25 °C to obtain the antibacterial protective makeup primer; the remaining steps are the same as in Example 2.

[0027] Comparative Example 4 The difference between Comparative Example 4 and Example 2 lies in step (2). Step (2) is modified as follows: Dissolve 15 g of chitosan in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain a chitosan aqueous solution; dissolve 15 g of sodium alginate in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain a sodium alginate aqueous solution; mix the chitosan aqueous solution and the sodium alginate aqueous solution in a mass ratio of 1:1 to obtain a mixed solution. First, add 1% of the total mass of the mixed solution of the plant polypeptide metal chelate. After stirring at 60 rpm for 20 min, add the photoinitiator I2959 to the solution. The addition amount of the photoinitiator is 0.1% of the volume of the mixed solution. Irradiate under a 100 W ultraviolet lamp for 6 min, and the ultraviolet intensity is 4800 μW / cm² to obtain hydrogel balls; put 50 g of hydrogel balls into 1 L of deionized water, add 3.5 g of lecithin, and slowly stir and react at 20 rpm for 2 h. After the reaction is completed, filter to obtain the filtrate, and wash it 3 times with deionized water to prepare composite hydrogel balls; the remaining steps are the same as those in Example 2.

[0028] Comparative Example 5 The difference between Comparative Example 5 and Example 2 lies in step (2). Step (2) is modified as follows: Dissolve 15 g of chitosan in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain a chitosan aqueous solution; dissolve 15 g of sodium alginate in 1 L of deionized water, dropwise add 110 g of methacrylic anhydride, and adjust the pH to 9 with a 0.5 mol / L aqueous sodium hydroxide solution. Stir at 60 rpm for 20 min to obtain a sodium alginate aqueous solution; mix the chitosan aqueous solution and the sodium alginate aqueous solution in a mass ratio of 1:1 to obtain a mixed solution. First, add 1% of the total mass of the mixed solution of the plant polypeptide metal chelate. After stirring at 60 rpm for 20 min, add 10% of calcium chloride based on the mass of sodium alginate, and mix and stir at 60 rpm for 30 min. Add the photoinitiator I2959 to the solution. The addition amount of the photoinitiator is 0.1% of the volume of the mixed solution. Irradiate under a 100 W ultraviolet lamp for 6 min, and the ultraviolet intensity is 4800 μW / cm² to obtain composite hydrogel balls; the remaining steps are the same as those in Example 2.

[0029] Effect Example The performance analysis results of a plant-derived polypeptide and a cosmetic containing the plant-derived polypeptide using Examples 1 to 3 and Comparative Examples 1 to 5 of the present invention are given in Table 1 and Table 2 below.

[0030] Table 1 Table 2 Sense of irritation Whether the facial skin is flushed Whether the skin around the eyes is flushed after contact Example 1 Among the 30 cases on the left cheek, none had a sense of irritation, while 4 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 5 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 6 cases on the right cheek had slight flushing and 1 case had flushing Example 2 Among the 30 cases on the left cheek, none had a sense of irritation, while 5 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 5 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 5 cases on the right cheek had slight flushing Example 3 Among the 30 cases on the left cheek, none had a sense of irritation, while 3 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 6 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 7 cases on the right cheek had slight flushing Comparative Example 1 Among the 30 cases on the left cheek, none had a sense of irritation, while 3 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 4 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact Comparative Example 2 Among the 30 cases on the left cheek, none had a sense of irritation, while 4 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 5 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 6 cases on the right cheek had slight flushing and 1 case had flushing Comparative Example 3 Among the 30 cases on the left cheek, none had a sense of irritation, while 4 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 5 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 7 cases on the right cheek had slight flushing Comparative Example 4 Among the 30 cases on the left cheek, none had a sense of irritation, while 3 cases on the right cheek had a slight sense of irritation Among the 30 cases on the left cheek, none had skin flushing, while 6 cases on the right cheek had slight flushing Among the 30 cases on the left cheek, none had flushing around the eyes after contact, while 7 cases on the right cheek had slight flushing Comparative Example 5 4 cases on the left cheek had a slight sense of irritation, and 4 cases on the right cheek had a slight sense of irritation 3 cases on the left cheek had slight skin flushing, and 6 cases on the right cheek had slight flushing 6 cases on the left cheek had no flushing around the eyes after contact, while 7 cases on the right cheek had slight flushing and 2 cases had flushing From the comparison of the experimental data on the antibacterial properties of the examples and the comparative examples, it can be found that in the present invention, mung bean protease is hydrolyzed to generate small peptides composed of three to six amino acids. Through the action of coordination covalent chemical bonds, a chemically stable complex of copper ions and mung bean small peptides is formed. Under appropriate conditions, groups such as amino groups on the main component arginine of the small peptide can act as ligands to provide electrons for metal copper ions, forming a stable plant polypeptide metal chelate; this metal chelate can act on the facial skin, improve its permeability, increase the penetration so that arginine can enter the skin interior, effectively stimulate mitochondrial activity, accelerate cell metabolism, thereby enhancing the resistance of cells to external stimuli and reducing inflammation; and the metal copper ions themselves have a certain antibacterial effect, and the two combine synergistically, making the skin after using the primer less susceptible to bacterial infection during the subsequent makeup application process. From the comparison of the experimental data on the protection of the examples and the comparative examples, it can be found that in the present invention, natural polymer chitosan and natural extract sodium alginate are used as matrices, and a hydrogel embedding the chelate is prepared by free radical polymerization, supplemented with calcium ion crosslinking to form hydrogel spheres with a polymer network structure built by two molecular chains, making the hydrogel spheres have good acid-base stability. The porous and rough surface structure of this hydrogel sphere provides binding sites, and the negatively charged lecithin electrostatically binds to the positively charged calcium ions, making lecithin stably loaded on the hydrogel spheres; during use, the hydrogel spheres are rubbed and squeezed, releasing the embedded plant polypeptide metal chelate, which acts on the skin and adsorbs lecithin on the skin surface to form a thin oil protection layer, making the subsequent makeup products of the primer less likely to have an irritating negative impact on the skin, thus achieving a protective effect and significantly reducing sensitivity and redness phenomena. As a plant extract, mung bean polypeptide has low antigenicity and will not cause allergic reactions even when directly acting on the inner part of the skin.

[0031] For those skilled in the art, it is obvious that the present invention is not limited to the details of the above exemplary embodiments, and without departing from the spirit or basic characteristics of the present invention, the present invention can be implemented in other specific forms. Therefore, from any point of view, the embodiments should be regarded as exemplary and non-limiting. The scope of the present invention is defined by the appended claims rather than the above description. Therefore, all changes falling within the meaning and scope of the equivalent elements of the claims are intended to be included in the present invention. Any reference signs in the claims should not be construed as limiting the claimed rights.

Claims

1. A cosmetic containing plant-derived polypeptides, wherein the cosmetic is a primer prepared by using composite hydrogel spheres that embed plant polypeptide metal chelates and simultaneously load lecithin as an effective factor, characterized in that: The method comprises the following preparation steps: (1) Take 40g of mung bean protein and dissolve it in 1L of deionized water. Pre-treat it in a 90℃ water bath for 20min. After the temperature drops to 55℃, adjust the pH to 9 with 0.5mol / L sodium hydroxide aqueous solution, add 5mL of alkaline protease, and keep the pH at 9 with 0.5mol / L sodium hydroxide aqueous solution. After hydrolysis for 4h, inactivate the enzyme in a 100℃ boiling water bath for 1h to obtain a hydrolyzate. Cool the hydrolyzate to 25℃, adjust the pH to 7 with 1mol / L hydrochloric acid aqueous solution, centrifuge at 5000rpm for 20min, and take the supernatant. Filter the supernatant at a pressure of 0.25MPa through an ultrafiltration membrane. The ultrafiltration membrane consists of an ultrafiltration membrane with a molecular weight cutoff of 5ku and 3ku. composition, obtain mung bean protein small molecule peptide filtrate; freeze-dry the mung bean protein small molecule peptide filtrate for later use; dissolve 50g of mung bean protein small molecule peptide in 1L of deionized water, adjust the pH to 6 with 1mol / L hydrochloric acid aqueous solution, add 5mgCuSO4·5H2O, stir at 200rpm for chelation reaction, the reaction temperature is 50℃, the reaction time is 80min, cool to 25℃ after the reaction, precipitate with 3L of anhydrous ethanol, react for 3.5h, centrifuge at 10000rpm for 15min, collect the precipitate, and wash with anhydrous ethanol 3 times; adjust the oven temperature to 65℃ for 1h, then reduce the temperature to 30℃ for 3h to obtain the plant polypeptide metal chelate; (2) Dissolve 15 g of chitosan in 1 L of deionized water, add 110 g of methacrylic anhydride dropwise, adjust the pH to 9 with a 0.5 mol / L sodium hydroxide aqueous solution, and stir at 60 rpm for 20 min to obtain a chitosan aqueous solution; Dissolve 15 g of sodium alginate in 1 L of deionized water, add 110 g of methacrylic anhydride dropwise, adjust the pH to 9 with a 0.5 mol / L sodium hydroxide aqueous solution, and stir at 60 rpm for 20 min to obtain a sodium alginate aqueous solution; Mix the chitosan aqueous solution and the sodium alginate aqueous solution in a mass ratio of 1:1 to obtain a mixed solution, first add 1% sodium alginate per 100 g of the total mass of the mixed solution, and then add 1% sodium alginate per 100 g of the total mass of the mixed solution. 1% of plant polypeptide metal chelate was added, stirred at 60rpm for 20min, and then calcium chloride accounting for 10% of the mass of sodium alginate was added, mixed and stirred at 60rpm for 30min, and photoinitiator I2959 was added to the solution, and the amount of the photoinitiator added was 0.1% of the volume of the mixed solution, and irradiated under a 100W ultraviolet lamp for 6min with an ultraviolet intensity of 4800μW / cm to obtain hydrogel balls; 50g of hydrogel balls were put into 1L of deionized water, 3.5g of lecithin was added, and the reaction was slowly stirred at 20rpm for 2h. After the reaction was completed, the filtrate was filtered and washed with deionized water for 3 times to obtain composite hydrogel balls; (3) Add 35g of deionized water, 4g of glycerol, 7g of butylene glycol, and 2.5g of ethanol into a water pot and heat it to 85°C, keep it for 10 min, cool it down to 65°C, add 2g of 1,2-pentanediol, and stir at 60rpm for 5 min to make a water pot material; then add 3.5g of cyclopentasiloxane and 2.5g of PEG-10 polydimethylsiloxane, 4g of trimethylsiloxysilicate, 7g of phenyl polytrimethicone, 4g of lauryl PEG-9 polydimethylsiloxane polydimethylsiloxane, 4g of glyceryl tri(ethylhexanoate), 4g of caprylyl polymethicone, 2g of polyglyceryl-2 isostearate, and 2g of composite hydrogel balls into a reaction pot, and homogenize and disperse at 20rpm for 20min to make a reaction pot material; heat it to 65°C, slowly add the water pot material into the reaction pot in 3 times, and stir at 20rpm for 10 min; when the temperature drops to 45°C with stirring, add 0.55g of phenoxyethanol, stir at 20rpm for another 10 min, and then cool to 25°C to obtain the antibacterial protective primer.

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