Moisturizing and repairing composition, application and cosmetics

Abstract

The present invention belongs to the field of daily chemicals and discloses a moisturizing and repairing composition comprising the following components by weight: 5-15% hydrolyzed keratin; 1-10% hydrolyzed rice protein; 0.1-5% schisandra fruit extract; and 1-10% olive leaf extract. The moisturizing and repairing composition, with hydrolyzed keratin and olive leaf extract as its core, performs the triple function of disulfide bond repair, moisturizing, and antibacterial, achieving basic moisturizing and repairing effects. The addition of hydrolyzed rice protein and schisandra fruit extract further enhances the disulfide bond repair and moisturizing effects of hair. The combination of the four components can achieve excellent antibacterial and antioxidant effects, maintain scalp health, and achieve two-way repair of hair and scalp. The present invention also discloses applications of the moisturizing and repairing composition and cosmetics.

Description

Technical Field

[0001] The present invention relates to the field of daily chemicals, and in particular to a moisturizing and repairing composition, application and cosmetics. Background Art

[0002] Hydrolyzed keratin is widely used in hair care because the vast majority of protein in human hair is keratin. In many cases, hair damage is caused by the breaking of disulfide bonds. Therefore, hydrolyzed keratin, based on its large number of disulfide bonds, can repair broken disulfide bonds and significantly repair damaged hair.

[0003] At the same time, according to existing research, hydrolyzed keratin also has a good water retention effect and improves hair elasticity.

[0004] Application number CN119326679A, for a patent application on an elastin serum that enhances hair toughness and elasticity, studies the effects of hydrolyzed keratin of different molecular weights on hair toughness and elasticity;

[0005] Application number CN118001211A, for a composition for repairing damaged hair and its preparation method, investigates the effects of cocodimonium hydroxypropyl hydrolyzed wheat keratin, hydrolyzed keratin, and soy peptides on hair repair. The application further utilizes olive oil, which, through its cholesterol and fatty acids, replenishes the cholesterol and fatty acids in the hair, increasing its oiliness and moisture retention. Olive oil adheres to the outer layer of hair fibers, forming a hydrophobic layer that locks in moisture, reduces moisture loss, and increases hair gloss. Olive oil also smoothes the hair scales, reduces friction, and softens the hair.

[0006] Through research on existing technologies, it can be found that the combination of multiple functional hydrolyzed proteins and other polypeptides combined with plant extracts with different functions can achieve a more obvious hair repair effect.

[0007] An important way for hair to be damaged is during the perming and dyeing process, which leads to the breakage of disulfide bonds and significantly reduces the water retention of hair. Therefore, in the process of hair repair, the two most important links to focus on are water retention and disulfide bond repair. At the same time, hair repair is not only for hair repair, but also for the improvement of scalp health, such as scalp oil control and scalp anti-oxidation. Only by ensuring the health of the scalp can the function of hair follicle cells be improved, the glossiness of new hair can be increased, hair graying can be avoided, and hair loss can be inhibited.

[0008] Therefore, the technical problem solved by the present invention is: how to propose a composition with relatively comprehensive functions from multiple perspectives and more significant functions such as hair repair and moisturizing.

[0009] At the same time, during the experiment, we also discovered some new and unexpected experimental phenomena, based on which we proposed this case. Summary of the Invention

[0010] The present invention aims to provide a moisturizing and repairing composition. The composition uses hydrolyzed keratin and olive leaf extract as cores, and has the triple effects of disulfide bond repair, moisturizing, and antibacterial, thereby achieving basic moisturizing and repairing effects. The composition is combined with hydrolyzed rice protein and schisandra fruit extract to further enhance the disulfide bond repair and moisturizing effects of the hair. At the same time, the combination of the four ingredients can achieve good antibacterial and antioxidant effects, maintain scalp health, and achieve two-way repair of the hair and scalp.

[0011] At the same time, the invention also discloses the application of the composition and cosmetics.

[0012] To achieve the above objectives, this application discloses:

[0013] A moisturizing and repairing composition comprising the following components in percentage by weight:

[0014] Hydrolyzed keratin 5-15%;

[0015] Hydrolyzed rice protein 1-10%;

[0016] Schisandra fruit extract 0.1-5%;

[0017] Olive leaf extract 1-10%.

[0018] The present invention uses hydrolyzed keratin and olive leaf extract as its core, which play a triple role in disulfide bond repair, moisturizing and antibacterial, achieving basic moisturizing and repair effects; combined with hydrolyzed rice protein and Schisandra chinensis fruit extract, it further enhances the disulfide bond repair and moisturizing effects of the hair. At the same time, the combination of the above four can achieve good antibacterial and antioxidant effects, maintain scalp health, and achieve two-way repair of hair and scalp.

[0019] More specifically:

[0020] Hydrolyzed keratin: It has the effect of moisturizing and repairing disulfide bonds;

[0021] Olive Leaf Extract: Has strong antibacterial and antioxidant properties; its antibacterial properties can improve scalp health and enhance hair quality; its hydroxytyrosol and oleuropein are the main antioxidant components that penetrate into the hair and repair damaged hair by assisting in the hydrolysis of keratin.

[0022] Through the above two components, hair repair and scalp care are achieved, and hair quality is improved;

[0023] Hydrolyzed rice protein: Rice protein contains 75%~90% alkali-soluble gluten. Alkali-soluble gluten is formed by many large molecular fragments through disulfide bonds, which are cross-linked and condensed with each other. After hydrolysis, it contains a large number of active disulfide bonds, which can further improve the disulfide bond repair function of hydrolyzed keratin.

[0024] Schisandra Fruit Extract: Schisandra is often used in shampoo products along with other Chinese herbal ingredients such as Gleditsia sinensis, Sapindus mukorossi, Thunbergia orientalis, Sophora flavescens, Eclipta prostrata, Polygonum multiflorum, Ganoderma lucidum, Ligustrum lucidum fruit, Morus alba, Chinese angelica, and aloe vera. Schisandra fruit extract is rich in vitamin C, vitamin E, schisandrin B, and polyphenols, which can scavenge free radicals. When used in combination with olive leaf extract, it can provide enhanced antioxidant benefits, helping to repair hair and improve scalp health.

[0025] At the same time, Schisandra chinensis fruit extract contains lignin, especially schisandrin B, which has been shown to have a certain inhibitory effect on Staphylococcus aureus (S. aureus), Candida albicans (C. albicans), Salmonella, Escherichia coli (E. coli), and Bacillus subtilis (B. subtilis). When used in combination with olive leaf extract, it has a more obvious antibacterial effect (Propionibacterium acnes); when the four raw materials of the present invention are combined, we found that it has a significant inhibitory effect on Staphylococcus aureus.

[0026] In summary, the combination of multiple active ingredients of the present invention has verified its excellence in hair repair, moisturizing, antibacterial and antioxidant functions, and is a very effective composition for achieving comprehensive repair and improvement of hair and scalp.

[0027] The above-mentioned moisturizing and repairing composition includes the following components in percentage by weight:

[0028] Hydrolyzed keratin 8-12%;

[0029] Hydrolyzed rice protein 3-7%;

[0030] Schisandra fruit extract 1-3%;

[0031] Olive leaf extract 2-6%.

[0032] The above-mentioned moisturizing and repairing composition further includes one or more of glycerin, 1,2-hexanediol, and p-hydroxyacetophenone, wherein the amount of glycerin is 15-25%; the amount of 1,2-hexanediol is 0.1-2%; and the amount of p-hydroxyacetophenone is 0.1-1%.

[0033] At the same time, the present invention also discloses the use of the moisturizing and repairing composition as described above to prepare an antibacterial agent.

[0034] The present invention has been tested on Propionibacterium acnes and Staphylococcus aureus, and it can be proved that the olive leaf extract and the schisandra fruit extract show synergy in the Propionibacterium acnes antibacterial experiment, and the hydrolyzed keratin, hydrolyzed rice protein, olive leaf extract and schisandra fruit extract show very obvious antibacterial synergy in the Staphylococcus aureus antibacterial experiment.

[0035] At the same time, the present invention also discloses a use of any of the above-mentioned moisturizing and repairing compositions to prepare hair cosmetics or cosmetics for use on the skin.

[0036] In addition, the present invention also discloses a hair cosmetic containing 0.01 to 30 wt % of any one of the above moisturizing and repairing compositions.

[0037] In the above-mentioned hair cosmetics, the hair cosmetics are one or more combinations of shampoo, conditioner, hair cream, hair essence, hair oil, dry hair spray, and hair mask.

[0038] Finally, the present invention also discloses a skin care cosmetic comprising 0.01 to 30 wt% of any of the above moisturizing and repairing compositions. Preferably, the cosmetic is an acne-removing cosmetic or a skin-moisturizing cosmetic.

[0039] This application has at least the following beneficial effects:

[0040] The composition of the present invention uses hydrolyzed keratin and olive leaf extract as its core, and has the triple effects of disulfide bond repair, moisturizing, and antibacterial, thereby achieving basic moisturizing and repairing effects. Hydrolyzed rice protein and schisandra fruit extract are added to further enhance the disulfide bond repair and moisturizing effects of the hair. Simultaneously, the combined use of the four ingredients can achieve good antibacterial and antioxidant effects, maintain scalp health, and achieve two-way repair of the hair and scalp. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 These are photos of the hair scale repair of Example 3 before and after the test;

[0042] Figure 2 This is a photo of the hair scale repair of Example 5 before testing;

[0043] Figure 3 This is a photo of the hair scale repair test of Example 5;

[0044] Figure 4 This is a photo of the hair scale repair of Comparative Example 6 before testing;

[0045] Figure 5This is a photo of the hair scale repair test of Example 6;

[0046] Figure 6 This is a photo of the chicken embryo chorioallantoic membrane test results. DETAILED DESCRIPTION

[0047] Below in conjunction with embodiments of the present invention, the present invention is clearly and completely described, in description of the present invention, it should be noted that, in the embodiment, the unrecited specific conditions person, carry out according to the condition of normal condition or manufacturer's suggestion. Reagents therefor or instrument are not recited manufacturer person, are the conventional products that can be obtained by commercial purchase. When not making special instructions, used part in the embodiments of the present invention is all weight part.

[0048] Source of raw materials

[0049] Hydrolyzed rice protein: Supplier is Guangzhou Huamiao Biotechnology Research Institute Co., Ltd., specification: 10kg / barrel, 100% hydrolyzed rice protein;

[0050] The preparation method comprises the following steps: pulverize rice in a rice powder to water ratio of 1:1, extract in a 90°C water bath with stirring for 3 hours; cool to 55°C, add α-amylase, and hydrolyze for 1 hour; then add papain and continue hydrolyzing for 1 hour; then raise the temperature to 90°C to inactivate the enzyme. Residue is removed by centrifugation, and ultrafiltration is performed using a membrane filter to collect the filtrate.

[0051] Hydrolyzed keratin: Supplier is Guangzhou Huamiao Biotechnology Research Institute Co., Ltd., specification: 10kg / barrel, 100% hydrolyzed keratin;

[0052] The preparation method is as follows: wool is washed with clean water to remove impurities and the moisture is controlled. The wool is degreased at room temperature for 24 hours at a weight ratio of 1:10. The degreased wool is then centrifuged to remove the degreaser. To the degreased wool, 5 BV of water is added, and the pH is adjusted to approximately 9 with NaOH. 1% w / w keratinase is added and enzymatically hydrolyzed at 45-50°C for 4-6 hours. The pH is then adjusted to 6-8 with citric acid. The wool is then heated to 80°C and kept warm for 10 minutes to inactivate the enzyme. The residue is then removed by centrifugation, and the filtrate is ultrafiltered through a 10 kDa filter membrane. The filtrate is then collected and spray-dried.

[0053] Schisandra chinensis fruit extract: Supplier is Guangzhou Huamiao Biotechnology Research Institute Co., Ltd., specification: 25kg / barrel;

[0054] The extraction method is as follows: Schisandra chinensis (fruit, water content <10.0%), add 10BV volume of 50vol% ethanol, stir and extract at 65℃ for 3h, centrifuge, concentrate the filtrate to alcohol-free (solid content is 25±10%), filter and sterilize.

[0055] Olive leaf extract: The supplier is Guangzhou Huamiao Biotechnology Research Institute Co., Ltd., specification: 25kg / barrel.

[0056] The extraction method is as follows: add 10 BV of water to olive leaves (dried leaves, moisture content <5.0%), stir and extract at 70°C for 3 hours, centrifuge, concentrate the filtrate to approximately 2 / 5 of the original volume, add 95% ethanol (1.5 times the volume of the concentrate) for precipitation, centrifuge, concentrate the filtrate to an alcohol-free state (solids content 35 ± 10%), filter, and sterilize.

[0057] Part I Antimicrobial Test

[0058] 1.1 Antibacterial experiment against Propionibacterium acnes

[0059] 1.1.1 Recipe Information

[0060] The formula table for the antibacterial experiment of Propionibacterium acnes is shown in Table 1 below;

[0061] Table 1 Formulation wt%

[0062] Hydrolyzed keratin Hydrolyzed rice protein Olive Leaf Extract Schisandra fruit extract water Sample 1 0 0 4 0 96 Sample 2 0 0 0 2 98 Sample 3 10 5 4 2 79

[0063] 1.1.2 Experimental methods

[0064] Take a suspension of Propionibacterium acnes and add it to each test sample (tested at the indicated concentration) and control sample (Propionibacterium acnes suspension + phosphate buffer) and mix thoroughly. Start timing. When the designated exposure time has elapsed, dilute the sample appropriately. Take two to three dilutions and place the test sample and control sample onto two separate plates. Pour Reinforced Clostridium Medium (RCM) into the plates and mix thoroughly. Once solidified, flip the plates over and incubate in an anaerobic incubator for 48 hours. Count the viable bacteria.

[0065] Calculation formula

[0066] Antibacterial rate = (AB) / A×100%;

[0067] Where: A—average colony count of control samples; B—average colony count of test samples.

[0068] 1.1.3 Experimental Results

[0069] The experimental results can be found in Table 2;

[0070] Table 2 Results of antibacterial test on Propionibacterium acnes

[0071] Project Name average value Control sample group Antibacterial rate Remark Sample 1 20000 60000 66.67% Original solution test, olive leaf extract concentration 4%, 10min Sample 2 29000 60000 51.67% Original solution test, Schisandra chinensis fruit extract concentration 2%, 10min Sample 3 120 60000 99.80% Original solution test, total concentration of the whole formula is 21%, 10min

[0072] Note: The stock solution described in this article refers to the formula prepared according to Table 1, Table 3, and Table 4.

[0073] 1.2 Staphylococcus aureus antibacterial test

[0074] 1.2.1 Recipe Information

[0075] The formula tables for the Staphylococcus aureus experiment are shown in Tables 3 and 4 below. In the first phase, the test was conducted according to the formula in Table 3, but the test results exceeded expectations. In order to study the reasons for the results, the test in Table 4 was conducted three weeks later, and the reasons for the phenomenon were preliminarily determined.

[0076] Table 3 Formulation wt%

[0077] Hydrolyzed keratin Hydrolyzed rice protein Olive Leaf Extract Schisandra fruit extract water Sample 1 0 0 4 0 96 Sample 2 0 0 0 2 98 Sample 3 10 5 4 2 79

[0078] Table 4 Formulation wt%

[0079] Hydrolyzed keratin Hydrolyzed rice protein Olive Leaf Extract Schisandra fruit extract water Sample 4 0 0 4 2 94 Sample 5 0 5 0 0 95 Sample 6 10 0 0 0 90

[0080] 2.1.2 Experimental methods

[0081] Take a Staphylococcus aureus suspension and add it to each test sample (tested at the noted concentration) and control sample (Staphylococcus aureus suspension + phosphate buffer) and mix thoroughly. Start timing. When the designated exposure time has elapsed, dilute the sample appropriately. Take two to three dilutions and place the test sample and control sample onto two separate plates. Pour nutrient agar medium over the mixture and mix thoroughly. Once solidified, flip the plates over and incubate in an incubator for 48 hours. Count the viable bacteria.

[0082] Calculation formula

[0083] Antibacterial rate = (AB) / A×100%;

[0084] Where: A—average colony count of control samples; B—average colony count of test samples.

[0085] Evaluation criteria: If the antibacterial rate is ≥50%~90%, the product has antibacterial effect; if the antibacterial rate is ≥90%, the product has a strong antibacterial effect.

[0086] 2.1.3 Experimental Results

[0087] The experimental results corresponding to Table 3 can be found in Table 5;

[0088] Table 5 Staphylococcus aureus antibacterial test results

[0089] Project Name average value Control sample group Antibacterial rate Remark Sample 1 72000 89000 19.10% Original solution test, olive leaf extract concentration 4%, 10min Sample 2 69000 89000 22.47% Original solution test, Schisandra chinensis fruit extract concentration 2%, 10min Sample 3 10 89000 99.99% Original solution test, total formula concentration 21%, 10min

[0090] The experimental results corresponding to Table 4 can be referred to Table 6;

[0091] Table 6 Staphylococcus aureus antibacterial test results

[0092] Project Name average value Control sample group Antibacterial rate Remark Sample 4 77000 83000 7.23% Original solution test, olive leaf extract concentration 4%, Schisandra chinensis fruit extract concentration 2%, 10min Sample 5 77000 83000 7.23% Stock solution test, hydrolyzed rice protein concentration 5%, 10min Sample 6 75000 83000 9.64% Original solution test, hydrolyzed keratin concentration 10%, 10min

[0093] Result analysis:

[0094] After many experiments, we found that:

[0095] 1. Olive leaf extract and Schisandra chinensis fruit extract have antibacterial antagonism;

[0096] 2. There is obvious synergy between hydrolyzed keratin, hydrolyzed rice protein, olive leaf extract and schisandra fruit extract.

[0097] The moisturizing and repairing composition of the present invention has a uniform and significant effect in inhibiting Staphylococcus aureus and Propionibacterium acnes. In particular, there is a significant synergy between the four bacteria in the case of Staphylococcus aureus.

[0098] Part 2 DPPH free radical scavenging rate test

[0099] 2.1 Formulation Information

[0100] Hydrolyzed keratin, hydrolyzed rice protein, Schisandra chinensis fruit extract, olive leaf extract, and glycerin were dispersed in deionized water and prepared into samples according to the formula in Table 7.

[0101] Table 7 Formulation Unit: wt%

[0102] Hydrolyzed keratin Hydrolyzed rice protein Schisandra fruit extract Olive Leaf Extract glycerin water Example 1 5 1 0.1 1 20 Add to 100 Example 2 10 5 2 4 20 Add to 100 Example 3 15 10 5 10 20 Add to 100 Comparative Example 1 10 0 0 4 20 Add to 100 Comparative Example 2 0 5 2 0 20 Add to 100 Comparative Example 3 10 5 0 4 20 Add to 100 Comparative Example 4 10 0 2 4 20 Add to 100

[0103] 2.2 DPPH free radical scavenging rate test method

[0104] (1) Treatment of control and test samples

[0105] Sample: Dilute with pure water to a sample concentration of 5%;

[0106] Positive control (vitamin E, purity ≥96%): Dilute with 95% ethanol to a positive control concentration of 0.1%;

[0107] Negative control: pure water.

[0108] (2) Test operation steps

[0109] Set up sample tubes, sample background tubes, DPPH tubes, and solvent background tubes. Set up three parallel tubes for each group. Add different reagent solutions to each of the four groups, shake gently, and let stand at room temperature for 5 minutes. Transfer each reaction solution into a 1 cm cuvette and measure the absorbance at 517 nm.

[0110] (3) Calculation formula

[0111] DPPH radical scavenging rate = (1-(T-T0) / (C-C0))*100;

[0112] Where: T—absorbance value of the sample tube, that is, the absorbance value of the solution after the sample reacts with DPPH;

[0113] T0—sample background absorbance value;

[0114] C—the average value of the absorbance of the DPPH tube three times, that is, the absorbance of the DPPH solution when no sample is added;

[0115] C0—Solvent background absorbance value.

[0116] 2.3 Test Results

[0117] The test results can be found in Table 8;

[0118] Table 8 DPPH free radical scavenging rate test results

[0119] DPPH free radical scavenging rate% Example 1 33.87 Example 2 47.72 Example 3 64.05 Comparative Example 1 26.84 Comparative Example 2 13.32 Comparative Example 3 23.16 Comparative Example 4 49.25 Negative control 0.82 Positive control 93.06

[0120] Result analysis:

[0121] 1. It can be seen from Examples 1 to 3 that as the test concentration gradually increases, the free radical scavenging effect gradually increases; at the same time, combined with the data of Comparative Example 4, we can clearly conclude that the improvement in free radical scavenging ability may be based on the combined effect of hydrolyzed keratin, olive leaf extract, and Schisandra chinensis fruit extract.

[0122] 2. As shown in the results of Example 2 and Comparative Example 4, hydrolyzed rice protein has almost no contribution to the lifting of free radical scavenging effect; As shown in the data of Example 2, Comparative Example 1 and Comparative Example 3, Schisandra chinensis fruit extract is an important guarantee for improving free radical scavenging effect; As shown in Comparative Example 1 and Comparative Example 2, in the present invention, hydrolyzed keratin and olive leaf extract provide basic free radical scavenging ability compared to Schisandra chinensis fruit extract and hydrolyzed rice protein.

[0123] Part III Hair Friction Test

[0124] 3.1 Formulation Information

[0125] Refer to 2.1 for recipe information.

[0126] 3.2 Test Method

[0127] Purpose and principle of the test

[0128] Because the cuticle is the outermost layer of the hair, damaged cuticles can slightly warp or crack at the edges, making the hair surface rough and dull. This increases friction and makes the hair feel frizzy. Normal hair has an intact cuticle that fits snugly to the hair shaft, resulting in a smooth surface and low friction. This method involves moving a specific wheel with a 200N weight at a constant speed from the root to the tip of the hair using a hair combing instrument. The friction is calculated based on the data. The change in friction before and after application of the sample is used to characterize the effectiveness of the cosmetic product for hair repair (for hair).

[0129] This test method is an in vitro method and is suitable for testing the efficacy of cosmetic repair (hair use).

[0130] Experimental materials and methods

[0131] Instruments and equipment: Combing tester (XJ810); Electronic balance (JJ1000);

[0132] Mildly damaged hair: length ≥10cm, net weight ≥6g;

[0133] Cleaning fluid: K12 (sodium lauryl sulfate);

[0134] Test environment: Test environment temperature 23℃±2℃, relative humidity 60%±10%.

[0135] Test methods

[0136] (1) Sample processing test

[0137] Dilute the sample to a concentration of 10% with first-grade water. Apply 200uL of sample evenly to each hair strand. Apply for about 30 seconds, let it sit for about 3-5 minutes, then place it in a constant temperature and humidity chamber for more than 4 hours until it is dry. Remove the hair strands and fix them in the test position to start the friction cycle test.

[0138] (2) Test operation steps

[0139] This test selected three slightly damaged human hair bundles, and used a combing instrument to test the friction of the hair bundles before and after the sample was used. Each hair bundle was rubbed 5 times in a cycle, and the friction value was recorded.

[0140] (3) Calculation formula

[0141] ;

[0142] Where: C0—friction force of baseline test;

[0143] Cs—Friction force of sample handling test.

[0144] 3.3 Test Results

[0145] The test results refer to Table 9;

[0146] Table 9 Hair bundle friction test results

[0147] Average value before use Average value after use Change rate / % Example 1 0.470 0.430 -8.51 Example 2 0.469 0.413 -11.94 Example 3 0.471 0.413 -12.31 Comparative Example 1 0.469 0.443 -5.54 Comparative Example 2 0.473 0.458 -3.17 Comparative Example 3 0.474 0.434 -8.44 Comparative Example 4 0.456 0.426 -6.58

[0148] Result analysis:

[0149] 1. As can be seen from Examples 1 to 3 above, the product of the present invention can achieve a good effect of reducing the friction of hair bundles;

[0150] 2. Comparative Examples 1, 2, 3, and 4 above demonstrate that each protein plays a significant role in reducing friction, as these proteins play a role in hair film formation and disulfide bond repair. The combination of olive leaf and hydrolyzed keratin effectively reduces friction, and the additional use of Schisandra chinensis fruit extract and hydrolyzed rice protein further significantly reduces friction. This demonstrates that the enhanced repair properties and antioxidant effects of the rich disulfide bonds in these two ingredients contribute significantly to friction reduction.

[0151] Part 4 Hair scale repair test

[0152] 4.1 Formulation Information

[0153] Refer to 2.1 for recipe information.

[0154] 4.2 Test Method

[0155] Purpose and principle of the test

[0156] Hair is composed of three parts: the cuticle, the cortex, and the medulla. The cuticle is the outermost layer of hair, tightly encasing the cortex in a scale-like pattern, protecting the hair fiber from mechanical and environmental damage. Daily exposure to perms, dyeing, sun exposure, combing, and hair dryers can damage the cuticle, causing it to curl or fall off, leading to dryness and loss of luster. This method damages hair tresses in vitro and observes the cuticles using a scanning electron microscope. Comparison of the cuticles in the sample group and the control group is used to evaluate the efficacy of the test sample in repairing the hair cuticle.

[0157] SEM is an electron microscopy technique that uses an electron beam to scan the surface of a sample, stimulating secondary electron imaging. Due to its high resolution, it can be used to analyze the surface morphology of hair and visually detect the extent of hair damage.

[0158] This test method is an in vitro method and is suitable for testing the efficacy of cosmetic repair (hair use).

[0159] Experimental materials and methods

[0160] Instruments and equipment: SEM scanning electron microscope;

[0161] Mildly damaged hair: length ≥10cm, net weight ≥6g;

[0162] Cleaning fluid: K12 (sodium lauryl sulfate);

[0163] Test environment: Test environment temperature 23℃±2℃, relative humidity 50%±10%.

[0164] Test methods

[0165] Sample processing test

[0166] Dilute the sample to 10% with first-grade water, and apply 200uL of sample evenly on the surface of each hair bundle for about 30 seconds. Let it stand for 3-5 minutes, then place it in a constant temperature and humidity chamber for more than 4 hours until the hair is dry.

[0167] The samples used in this experiment are Example 2, Comparative Example 3, and Comparative Example 4.

[0168] 4.3 Test Results

[0169] The photos before and after the test of Example 2 can be seen Figure 1 ;

[0170] The photos before and after the test of Comparative Example 3 can be seen Figure 2 and Figure 3 ;

[0171] The photos before and after the test of Comparative Example 4 can be seen Figure 4 and Figure 5 ;

[0172] The test shows that the effect of the composition of the present invention is significantly better than that of Comparative Example 3 and Comparative Example 4, which indicates that the composition of the present invention has significant advantages in repairing hair scales.

[0173] Part V: Formula Safety Test

[0174] Chicken chorioallantoic membrane test

[0175] Six embryos were used in this test. The condition of the chorioallantoic membrane was recorded using a camera. The test sample (Example 3, diluted to 2%) was added to the chorioallantoic membrane of the chicken embryos. The time of sample addition was recorded, and the air chamber was covered with moistened plastic wrap. The chicken embryos were then transferred to a constant temperature and humidity chamber for incubation. The extent of each toxic effect was observed and photographed.

[0176] Bleeding, coagulation, and vascular melting were observed and recorded, and scored according to their severity.

[0177] The endpoint evaluation method was used to calculate the endpoint evaluation (ES), with the result rounded to two decimal places. The score of each chicken embryo was the sum of the degree of bleeding, coagulation and vascular lysis observed in each chicken embryo. ES was the average of the mathematical sums obtained for 6 chicken embryos.

[0178] The experimental results can be found in Table 10;

[0179] Table 10 Chicken embryo chorioallantoic membrane test results

[0180] Serial number Sample name Treatment Results (ES) 1 Negative control (0.9% saline) / 0.00 2 Positive control (1% SDS) / 18.00 3 Example 3 Diluted to 2% 2.33

[0181] Test photos visible Figure 6 ;

[0182] The above results show that the formulation of the present invention is non-irritating.

[0183] Application Example 1

[0184] Based on the moisturizing and repairing composition prepared in Example 2, a portion of water was replaced with 1,2-hexanediol and p-hydroxyacetophenone to obtain a hair conditioner (Jifuyin). The specific preparation steps are as follows:

[0185] 1. Pre-preparation phase: Dissolve glycerol, 1,2-hexanediol and p-hydroxyacetophenone in advance and stir to mix evenly;

[0186] 2. In the main pot, add hydrolyzed keratin, hydrolyzed rice protein, schisandra fruit extract, olive leaf extract and purified water, stir to mix evenly, add the pre-made phase, stir evenly, and sterilize at 80±5℃ for 30min.

[0187] The specific components of a hair conditioner are shown in Table 11;

[0188] Table 11: Components of a hair conditioner

[0189] Components Addition amount (wt%) Hydrolyzed keratin 10 Hydrolyzed rice protein 5 Schisandra fruit extract 2 Olive Leaf Extract 4 glycerin 20 1,2-Hexanediol 0.5 p-Hydroxyacetophenone 0.5 water 58

[0190] Application Example 2

[0191] A hair conditioner, the specific formula is shown in Table 12, and the preparation method is:

[0192] 1. Add the raw materials of phase A to deionized water at room temperature while stirring and dispersing evenly. Heat to 80-85 degrees Celsius and keep warm for 15 minutes until it becomes a transparent liquid without particles.

[0193] 2. At 80-85°C, add the ingredients of phase B, stir to dissolve completely, homogenize for 3-5 minutes, and start cooling;

[0194] 3. Cool down to 45-50℃, add Phase C ingredients and stir well.

[0195] Table 12 Conditioner formula

[0196]

[0197] Application Example 3

[0198] A shampoo, the specific formula is shown in Table 13, and the preparation method is:

[0199] 1. Stir the raw material of phase A in deionized water at room temperature until it becomes a transparent liquid without particles, then start heating it to 80-85℃;

[0200] 2. Add Phase B ingredients at 80-85°C and stir to dissolve completely;

[0201] 3. Cool down to 45-50℃, add Phase C ingredients and stir well.

[0202] Table 13 Shampoo formula

[0203]

[0204] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and it is intended that equivalents falling within the claims be included. All variations within the meaning and scope of the elements are encompassed by the present invention. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. An antibacterial moisturizing repair composition, characterized in that: The composition includes the following components in weight percentage: Hydrolyzed keratin 8-12%; Hydrolyzed rice protein 3-7%; Schisandra fruit extract 1-3%; Olive leaf extract 2-6%; The composition is used for preparing hair cosmetics or cosmetics acting on the skin, and the composition has an antibacterial effect on Staphylococcus aureus.

2. The antibacterial moisturizing repair composition according to claim 1, characterized in that It also includes one or more of glycerol, 1,2-hexanediol, and p-hydroxyacetophenone, wherein the amount of glycerol is 15-25%; the amount of 1,2-hexanediol is 0.1-2%; and the amount of p-hydroxyacetophenone is 0.1-1%.

3. Use of the antibacterial, moisturizing and repairing composition according to claim 1 or 2 in the preparation of an antibacterial agent against Staphylococcus aureus.

4. Use of the antibacterial, moisturizing and repairing composition according to claim 1 or 2 in preparing hair cosmetics or cosmetics for use on the skin.

5. A hair cosmetic, characterized in that: Contains 0.01 to 30 wt % of the antibacterial, moisturizing and repairing composition according to claim 1 or 2.

6. The hair cosmetic according to claim 5, characterized in that The hair cosmetics are one or more combinations of shampoo, hair cream, hair essence, hair oil, dry hair spray, and hair mask.

7. A cosmetic for skin care, characterized in that: Contains 0.01 to 30 wt % of the antibacterial, moisturizing and repairing composition according to claim 1 or 2.

8. The cosmetic according to claim 7, characterized in that The cosmetics are cosmetics for removing acne or for moisturizing the skin.

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