Preparation method and extraction device of skincare radix stephaniae tetrandrae extract

Abstract

The invention discloses a preparation method and an extraction device of a skincare radix stephaniae tetrandrae extract. The preparation method comprises the following steps: crushing and sieving a cleaned and dried radix stephaniae tetrandrae medicinal material; then taking coarse powder of stephania tetrandra, adding an acidic aqueous solution, putting the mixture into an extraction device for double extraction of microjet and ultrasonic waves, and separating out clear liquid after the extraction is completed; directly adsorbing the clear liquid through pretreated macroporous cation exchange resin D72, washing the macroporous cation exchange resin D72 by using the same amount of distilled water after adsorption, and eluting and adsorbing a saturated resin column by using an ammonia water-ethanol solution until no alkaloid reaction exists in an eluent flowing out; the preparation method comprises the following steps: firstly, extracting the white tetrandrine, concentrating the eluent, then dissolving with an ethanol supersaturated solution, and repeatedly recrystallizing twice to obtain the high-purity white tetrandrine. Through the combination of microjet and ultrasonic wave, the active ingredients in the radix stephaniae tetrandrae can be effectively and efficiently extracted, and the generation of wastewater and the use of a solvent are reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of extraction of Stephania tetrandra, and specifically relates to a preparation method and an extraction device for a Stephania tetrandra extract for skin care. Background Art

[0002] The extract of Stephania tetrandra root has various effects in skin care products, mainly including anti-inflammatory, astringent, and anti-hair loss and hair nourishing effects. The extract of Stephania tetrandra root can be used as an active ingredient in anti-inflammatory agents, astringents, and anti-hair loss and hair nourishing products.

[0003] In the prior art, two representative methods for the extraction of Stephania tetrandra alkaloids are: (1) the current industrial extraction method: soaking with 0.6% sulfuric acid, precipitating the leaching solution with lime milk, drying the precipitate, pulverizing, extracting, and purifying, which can be used to prepare tetrandrine and fangchinoline. (2) Ethanol reflux extraction and improved method: reflux extraction with 85-95% ethanol, adding acid to precipitate and remove the fat-soluble impurities in the ethanol concentrate, alkalizing the acidified aqueous solution with ammonia water, and then repeatedly extracting with chloroform to remove the water-soluble impurities. After recovering chloroform to obtain the total alkaloids, they are refined with acetone. However, in Method 1, a large amount of wastewater will be generated, the extraction rate of tetrandrine will be reduced because it is difficult to completely precipitate, and the water-soluble alkaloids are difficult to recover. In Method 2, the solvent loss is large and the cost is high. Summary of the Invention

[0004] In order to solve the above deficiencies in the prior art, the present invention provides a preparation method and an extraction device for a Stephania tetrandra extract for skin care.

[0005] In order to achieve the above technical effects, the present invention adopts the following solutions:

[0006] A preparation method for a Stephania tetrandra extract for skin care includes the following steps:

[0007] S1. Pulverize and sieve the Stephania tetrandra medicinal materials after cleaning and drying.

[0008] S2. Take the Stephania tetrandra coarse powder, add an acidic aqueous solution with a pH value of 3.5-4.5, and then place it in an extraction device for double extraction of microfluidics and ultrasonic waves. After the extraction is completed, separate the clear liquid.

[0009] S3. Take the above clear liquid and directly adsorb it with pretreated macroporous cation exchange resin D72. After adsorption, first rinse the macroporous cation exchange resin D72 with an equal amount of distilled water, and then elute the saturated resin column with a 0.4-0.5 mol / L ammonia ethanol solution at a flow rate of 10 mL / min until the eluate shows no alkaloid reaction.

[0010] S4. Concentrate the eluate, then dissolve it with an ethanol supersaturated solution, and recrystallize it twice repeatedly to obtain high-purity white tetrandrine.

[0011] Preferred technical solution: In step S2, the material ratio of the crude Stephania tetrandra powder to the acidic aqueous solution is 1:5 to 1:7.

[0012] Preferred technical solution: In step S2, the power of the ultrasonic wave is 500 W, and the extraction time is 60 - 90 min.

[0013] Preferred technical solution: The pretreatment method of the macroporous cation exchange resin D72 is as follows: Immerse the macroporous cation exchange resin D72 in absolute ethanol for 24 h, and change the absolute ethanol solution every 3 h, and continuously stir to make the ethanol solution fully contact with the macroporous cation exchange resin D72 and drive away the air. After complete immersion, rinse with deionized water until there is no ethanol smell, then soak in 5% hydrochloric acid for 3 - 4 h, then rinse with deionized water until neutral, then soak in 5% sodium hydroxide solution for 3 - 4 h, then rinse with deionized water until neutral, and then place it in a drying oven for drying and standby.

[0014] An extraction device, comprising a mixing part, an extraction part and a centrifugation part connected in sequence;

[0015] The mixing part includes a mixing tank. The upper end of the mixing tank is provided with a feeding port, the lower end of the mixing tank is provided with a first discharge port. The first discharge port is provided with a valve one and the first discharge port is connected with a first feeding pipe. A stirring mechanism is arranged in the mixing tank;

[0016] The extraction part includes an extraction tank. The first feeding pipe is connected to the side wall of the extraction tank. The lower end of the extraction tank is provided with a second discharge port. The second discharge port is provided with a valve two and the second discharge port is connected with a second feeding pipe. A first delivery pump is connected to the second feeding pipe. The extraction tank is connected with a micro - jet mechanism and an ultrasonic mechanism;

[0017] The centrifugation part includes a frame. A rotating centrifugation tank is installed on the frame. A ring gear is fixedly arranged on the outer wall of the centrifugation tank. A motor is installed on the frame. The output end of the motor is provided with a gear meshed with the ring gear. The lower end of the centrifugation tank is provided with a third discharge port. The third discharge port is provided with a valve three. The upper end of the centrifugation tank is arranged in an upward - arched arc - shaped. A through - hole is opened at the center of the upper end of the centrifugation tank. A feeding pipe and a liquid - taking pipe are inserted into the through - hole. The feeding pipe is fixed on the frame and the upper end of the feeding pipe is connected with the second feeding pipe. The liquid - taking pipe is arranged on the frame through a lifting mechanism, and the upper end of the liquid - taking pipe is connected with a third feeding pipe. The third feeding pipe is connected with a second delivery pump.

[0018] Preferred technical solution: The stirring mechanism includes an upper bracket and a lower bracket arranged in the mixing tank. The upper bracket is arranged close to the upper end of the mixing tank, and the lower bracket is arranged close to the lower end of the mixing tank. A vertically arranged guiding circular tube is connected between the upper bracket and the lower bracket. The upper and lower ends of the guiding circular tube are through. There are two symmetrically arranged guiding chutes on the side wall of the guiding circular tube. The guiding chutes are wound around the guiding circular tube along the length direction of the guiding circular tube. A sliding driving block is arranged in the guiding circular tube in a matching manner. Two stirring rods are fixedly arranged on both sides of the driving block and extend out of the guiding circular tube through the two guiding chutes respectively. A vertically arranged cylinder 1 is installed at the top of the mixing tank. The telescopic rod of the cylinder 1 extends downward into the guiding circular tube and is rotatably connected to the center of the driving block.

[0019] Preferred technical solution: The micro-jet mechanism includes a nozzle fixedly arranged on the side wall of the extraction tank. The nozzle is arranged close to the upper end of the extraction tank. A horizontally connected high-pressure air duct and a compression duct are arranged in the nozzle. The high-pressure air duct extends outward and is connected to a connection port arranged at the outer end of the nozzle. The compression duct extends toward the extraction tank and is connected to a jet port arranged on the inner wall of the extraction tank. The connection port is connected to a gas tank through a gas supply pipe. A high-pressure pump is connected to the gas supply pipe. A feed port is arranged at the upper end of the nozzle. The feed port is vertically connected to the compression duct through a feed duct and is connected to a feed pipe 1.

[0020] Preferred technical solution: The ultrasonic mechanism includes an ultrasonic controller, an ultrasonic transducer, an ultrasonic amplitude transformer, and a tool head. The ultrasonic transducer is fixedly installed at the upper end of the extraction tank. The tool head is located in the extraction tank. The ultrasonic transducer is connected to the tool head through an ultrasonic amplitude transformer. The ultrasonic transducer is connected to the ultrasonic controller through an electric wire.

[0021] Preferred technical solution: A circular rotating edge is fixedly connected to the outer wall of the centrifugal tank. The center of the rotating edge is located on the rotation axis of the centrifugal tank. A circular rotating groove is formed on the frame. The rotating groove is matched with the rotating edge, and the rotating edge is embedded in the rotating groove and arranged to slide.

[0022] Preferred technical solution: The lifting mechanism includes a cylinder 2 vertically installed on the frame. The telescopic rod of the cylinder 2 extends downward. The liquid extraction pipe is installed on the telescopic rod of the cylinder 2 through a connecting frame.

[0023] Compared with the prior art, the beneficial effects are as follows:

[0024] In the present invention, microfluidics is used to break and mix Stephania tetrandra under high pressure and shear force. The cell walls of Stephania tetrandra are ruptured by strong mechanical action, thereby releasing the internal active ingredients. At the same time, the cavitation effect of ultrasonic waves is utilized to destroy the cells of plant medicinal materials, making it easier for the solvent to penetrate into the cells. Moreover, the strong vibration of ultrasonic waves can transmit huge energy to the extracted medicinal materials and solvent, causing them to move at high speed, strengthening the release, diffusion, and dissolution of intracellular substances. Compared with the prior art, the present invention can effectively and efficiently extract the active ingredients from Stephania tetrandra by combining microfluidics and ultrasonic waves, and reduce the generation of wastewater and the use of solvents. Description of the Drawings

[0025] Figure 1 It is a schematic diagram of the steps of the preparation method of the present invention.

[0026] Figure 2 It is a schematic cross-sectional view of the extraction device in the present invention.

[0027] Figure 3 It is a schematic cross-sectional view of the stirring mechanism in the present invention.

[0028] Figure 4 It is a schematic diagram of the guiding circular tube structure in the present invention.

[0029] Figure 5 It is a schematic diagram of the connection between the driving block and the stirring rod in the present invention.

[0030] Figure 6 It is a schematic cross-sectional view of the nozzle in the present invention.

[0031] Figure 7 It is a schematic cross-sectional view of the ultrasonic mechanism in the present invention.

[0032] Reference numerals: 1, mixing part; 101, mixing tank; 1011, feeding port; 1012, first discharging port; 1013, first valve; 102, first feeding pipe; 103, stirring mechanism; 1031, upper bracket; 1032, lower bracket; 1033, guiding circular pipe; 1034, guiding slideway; 1035, driving block; 1036, stirring rod; 1037, first cylinder; 2, extraction part; 201, extraction tank; 2011, second discharging port; 2012, second valve; 202, second feeding pipe; 203, first transfer pump; 204, micro-jet mechanism; 2041, nozzle; 2042, high-pressure air duct; 2043, compression duct; 2044, connection port; 2045, injection port; 2046, feeding port; 2047, feeding duct; 2048, gas tank; 2049, gas supply pipe; 205, ultrasonic mechanism; 2051, ultrasonic controller; 2052, ultrasonic transducer; 2053, ultrasonic horn; 2054, tool head; 3, centrifugal part; 301, frame; 3011, rotating groove; 302, centrifugal tank; 3021, third discharging port; 3022, third valve; 3023, through hole; 3024, rotating edge; 3025, gear ring; 303, motor; 3031, gear; 304, injection pipe; 305, liquid taking pipe; 306, third feeding pipe; 307, second transfer pump; 308, second cylinder. Detailed implementation manners

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments.

[0034] A preparation method and extraction device for Stephania tetrandra extract for skin care, comprising:

[0035] S1. Crush the cleaned and dried Stephania tetrandra medicinal materials and pass through a 100-mesh sieve;

[0036] S2. Take the Stephania tetrandra coarse powder, add 5 to 7 times of acidic aqueous solution with a pH value of 3.5 to 4.5. The acidic aqueous solution is preferably hydrochloric acid, place it in the extraction device for double extraction of micro-jet and ultrasonic wave. The power of the ultrasonic wave is 500 W, the extraction time is 60 to 90 min, and after the extraction is completed, the clear liquid is separated;

[0037] S3. Take the above-mentioned supernatant and directly adsorb it with the pretreated macroporous cation exchange resin D72. After adsorption, first rinse the macroporous cation exchange resin D72 with an equal amount of distilled water, and then elute the saturated resin column with an ammonia ethanol solution of 0.4 - 0.5 mol / L at a flow rate of 10 mL / min until no alkaloid reaction occurs in the eluate. Preferably, the pretreatment method of the macroporous cation exchange resin D72 is as follows: Immerse the macroporous cation exchange resin D72 in absolute ethanol for 24 h, and change the absolute ethanol solution every 3 h, and continuously stir to make the ethanol solution fully contact with the macroporous cation exchange resin D72 and expel air. After complete immersion, rinse with deionized water until there is no ethanol smell, then soak in 5% hydrochloric acid for 3 - 4 h, then rinse with deionized water until neutral, then soak in 5% sodium hydroxide solution for 3 - 4 h, then rinse with deionized water until neutral, and then place it in an oven to dry for later use.

[0038] S4. Concentrate the eluate, then dissolve it with an ethanol supersaturated solution, and recrystallize it twice repeatedly to obtain high-purity tetrandrine.

[0039] In the present invention, microfluidics is used to break and mix Stephania tetrandra under high pressure and shear force. Through strong mechanical action, the cell walls of Stephania tetrandra are ruptured, thereby releasing the internal active ingredients. At the same time, the cavitation effect of ultrasonic waves is utilized to destroy the cells of plant medicinal materials, making it easier for the solvent to penetrate into the cells. At the same time, the strong vibration of ultrasonic waves can transmit huge energy to the extracted medicinal materials and solvents, making them move at high speed, strengthening the release, diffusion and dissolution of intracellular substances. Compared with the prior art, the present invention can effectively and efficiently extract the active ingredients in Stephania tetrandra through the combination of microfluidics - ultrasonic waves, and reduce the generation of wastewater and the use of solvents.

[0040] The extraction device described above includes a mixing part 1, an extraction part 2 and a centrifugation part 3 connected in sequence. The mixing part 1 is used to mix the crude powder of Stephania tetrandra with an acidic aqueous solution evenly. The extraction part 2 is used to extract the active ingredients in the crude powder of Stephania tetrandra, mainly for extracting tetrandrine. The centrifugation part 3 is used to centrifuge the mixed solution after extraction to separate the supernatant.

[0041] The mixing part 1 includes a mixing tank 101. There is a feeding port 1011 at the upper end of the mixing tank 101. The crude powder of Stephania tetrandra and the acidic aqueous solution are put into the mixing tank 101 from the feeding port 1011. There is a first discharge port 1012 at the lower end of the mixing tank 101. A valve 1013 is provided at the first discharge port 1012 and the first discharge port 1012 is connected to a first feeding pipe 102. A stirring mechanism 103 is provided in the mixing tank 101. After being stirred by the stirring mechanism 103, the crude powder of Stephania tetrandra and the acidic aqueous solution are mixed evenly. Then, open the valve 1013 and send the mixed solution into the extraction part 2 through the first feeding pipe 102 for extraction.

[0042] The stirring mechanism 103 includes an upper bracket 1031 and a lower bracket 1032 disposed in the mixing tank 101. Both the upper bracket 1031 and the lower bracket 1032 are fixedly arranged. The upper bracket 1031 is disposed close to the upper end of the mixing tank 101, and the lower bracket 1032 is disposed close to the lower end of the mixing tank 101. A vertically arranged guiding circular tube 1033 is connected between the upper bracket 1031 and the lower bracket 1032. The upper and lower ends of the guiding circular tube 1033 are through holes, and the upper and lower ends of the guiding circular tube 1033 respectively penetrate through the upper bracket 1031 and the lower bracket 1032. Two symmetrically arranged guiding chutes 1034 are provided on the side wall of the guiding circular tube 1033. The depth direction of the guiding chute 1034 penetrates through the side wall of the guiding circular tube 1033. The length direction of the guiding chute 1034 is spirally wound around the guiding circular tube 1033 along the length direction of the guiding circular tube 1033. A sliding driving block 1035 is arranged in the guiding circular tube 1033 in a matching manner. The driving block 1035 is circular in shape and can rotate in the guiding circular tube 1033 and also slide along the length direction of the guiding circular tube 1033. Two stirring rods 1036 are fixedly arranged on both sides of the driving block 1035 and respectively extend out of the guiding circular tube 1033 through the two guiding chutes 1034. The stirring rods 1036 are horizontally arranged. A vertically arranged cylinder 1037 is installed at the top of the mixing tank 101. The telescopic rod of the cylinder 1037 extends downward into the guiding circular tube 1033 and is rotatably connected to the center of the driving block 1035.

[0043] The telescopic rod of the cylinder 1037 drives the driving block 1035 to move vertically in the guiding circular tube 1033. During the vertical movement of the guiding circular tube 1033, since the two stirring rods 1036 on both sides respectively pass through the two guiding chutes 1034, under the restriction of the guiding chutes 1034, the stirring rods 1036 rotate spirally on the guiding circular tube 1033 along the guiding chutes 1034. Thus, the cylinder 1037 drives the driving block 1035 to reciprocate in the guiding circular tube 1033, enabling the two stirring rods 1036 to rotate around the guiding circular tube 1033 while moving vertically. Therefore, stirring can be performed at different heights in the mixing tank 101. Compared with the traditional fixed paddle that can only stir at a fixed height, the stirring of this solution can improve the stirring efficiency.

[0044] The extraction unit 2 includes an extraction tank 201. The first feed pipe 102 is connected to the side wall of the extraction tank 201. A second discharge port 2011 is provided at the lower end of the extraction tank 201. A second valve 2012 is provided at the second discharge port 2011, and a second feed pipe 202 is connected to the second discharge port 2011. A first delivery pump 203 is connected to the second feed pipe 202. The extraction tank 201 is connected to a micro-jet mechanism 204 and an ultrasonic mechanism 205. The crude powder mixture of Stephania tetrandra is fed into the extraction tank 201 through the first feed pipe 102. After double extraction by the micro-jet mechanism 204 and the ultrasonic mechanism 205, the second valve 2012 is opened, and the extracted mixture is fed into the centrifugation unit 3 through the second feed pipe 202 for centrifugal separation.

[0045] The micro-jet mechanism 204 includes a nozzle 2041 fixedly provided on the side wall of the extraction tank 201. The nozzle 2041 is arranged near the upper end of the extraction tank 201. A high-pressure air passage 2042 and a compression passage 2043 are horizontally connected inside the nozzle 2041. The high-pressure air passage 2042 extends outward and is connected to a connection port 2044 provided at the outer end of the nozzle 2041. The compression passage 2043 extends toward the extraction tank 201 and is connected to a jet port 2045 provided on the inner wall of the extraction tank 201. The connection port 2044 is connected to a gas tank 2048 through a gas supply pipe 2049. A high-pressure pump is connected to the gas supply pipe 2049. An inlet port 2046 is provided at the upper end of the nozzle 2041. The inlet port 2046 is vertically connected to the compression passage 2043 through a feed passage 2047. The inlet port 2046 is connected to the first feed pipe. An exhaust hole is provided on the extraction tank 201.

[0046] The gas in the gas tank 2048 is fed into the high-pressure air passage 2042 through the pressurization of the high-pressure pump, and then enters the compression passage 2043. The inner diameter of the compression passage 2043 is much smaller than the inner diameter of the high-pressure air passage 2042, so that the flow rate and pressure of the air flow in the compression passage 2043 are greatly increased. The air flow in the compression passage 2043 rapidly flows horizontally along the compression passage 2043 and then enters the extraction tank 201. Due to the high-speed flow of the air flow in the compression passage 2043, a negative pressure is formed in the feed passage 2047, attracting the mixture in the mixing tank 101 to enter the compression passage 2043 after passing through the first feed pipe 102, the inlet port 2046 and the feed passage 2047. Then the mixture is mixed with the high-pressure air flow in the compression passage 2043 and flows at a high speed along the high-pressure air flow, and is sprayed into the extraction tank 201 at the jet port 2045. Thus, the Stephania tetrandra is broken and mixed by high pressure and shear force, and the cell wall of Stephania tetrandra is efficiently broken by strong mechanical action, thereby releasing the internal active ingredients.

[0047] The ultrasonic mechanism 205 includes an ultrasonic controller 2051, an ultrasonic transducer 2052, an ultrasonic horn 2053, and a tool head 2054. The ultrasonic transducer 2052 is fixedly installed at the upper end of the extraction tank 201. The tool head 2054 is located inside the extraction tank 201. The ultrasonic transducer 2052 is connected to the tool head 2054 through the ultrasonic horn 2053. The ultrasonic transducer 2052 is connected to the ultrasonic controller 2051 through an electric wire. The ultrasonic mechanism 205 is used to perform ultrasonic extraction on the Stephania tetrandra mixture in the extraction tank 201.

[0048] The centrifugation part 3 includes a frame 301. A rotating centrifugation tank 302 is installed on the frame 301. The centrifugation tank 302 is made of a transparent glass tank body, which is convenient for observing the state of the mixture in the centrifugation tank 302. An annular rotating edge 3024 is fixedly connected to the outer wall of the centrifugation tank 302. The center of the rotating edge 3024 is located on the rotation axis of the centrifugation tank 302. An annular rotating groove 3011 is formed on the frame 301. The rotating groove 3011 matches the rotating edge 3024, and the rotating edge 3024 is embedded in the rotating groove 3011 and slidably arranged. A ring gear 3025 is fixedly provided on the outer wall of the centrifugation tank 302. A motor 303 is installed on the frame 301. A gear 3031 meshing with the ring gear 3025 is provided at the output end of the motor 303. A discharge port three 3021 is provided at the lower end of the centrifugation tank 302. A valve three 3022 is provided at the discharge port three 3021. The upper end of the centrifugation tank 302 is arranged in an upwardly arched arc shape. A through hole 3023 is formed at the center of the upper end of the centrifugation tank 302. A feeding pipe 304 and a liquid extraction pipe 305 are inserted into the through hole 3023. Both the feeding pipe 304 and the liquid extraction pipe 305 are made of rigid materials and are vertically arranged. The feeding pipe 304 is fixed on the frame 301, and the upper end of the feeding pipe 304 is connected to the feeding pipe two 202. The liquid extraction pipe 305 is arranged on the frame 301 through a lifting mechanism, and the upper end of the liquid extraction pipe 305 is connected to a feeding pipe three 306. The feeding pipe three 306 is connected to a delivery pump two 307.

[0049] The Stephania tetrandra mixture after extraction is sent into the feeding pipe 304 through the feeding pipe two 202 and then into the extraction tank 201 from the feeding pipe 304. The motor 303 drives the centrifugation tank 302 to rotate at a high speed through the gear 3031 and the ring gear 3025, so as to centrifuge the mixture in the centrifugation tank 302, separating the clear liquid and the turbid liquid of the mixture. The turbid liquid is located in the lower layer and the clear liquid is located in the upper layer. Then, the lower end of the liquid extraction pipe 305 is lowered to the junction of the clear liquid and the turbid liquid through the lifting mechanism, and the delivery pump two 307 is started to extract the upper clear liquid through the feeding pipe three 306. The feeding pipe three 306 is arranged as a flexible pipe. After the extraction is completed, the valve three 3022 is opened, and the turbid liquid is discharged through the discharge port three 3021.

[0050] The discharge port three 3021 can be connected to the mixing tank 101 through the feed pipe four. The feed pipe four and the discharge port three 3021 are connected through a rotary joint. A delivery pump three is connected to the feed pipe four to send the turbid liquid back into the mixing tank 101 again. After adding the acidic aqueous solution, the aforementioned extraction is carried out again, and the extraction is repeated, thereby improving the extraction rate of the crude powder of Stephania tetrandra.

[0051] The lifting mechanism includes a cylinder two 308 vertically installed on the frame 301. The telescopic rod of the cylinder two 308 is arranged downward, and the liquid extraction pipe 305 is installed on the telescopic rod of the cylinder two 308 through a connecting frame. The cylinder two 308 is used to drive the liquid extraction pipe 305 to move up and down.

[0052] The edge of the through hole 3023 at the upper end of the centrifugal tank 302 is bent downward. During the centrifugation process, when the mixed liquid moving upward along the side wall of the centrifugal tank 302 reaches the upper end of the centrifugal tank 302, it is guided by the downward bending of the edge of the through hole 3023, so that the mixed liquid falls back into the centrifugal tank 302 to avoid flying out from the through hole 3023.

[0053] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by the terms "upper", "lower", "top", "bottom", "inner", "outer", etc. is based on the orientation or positional relationship shown in the drawings, or the orientation or positional relationship in which the product of the invention is usually placed during use, or the orientation or positional relationship commonly understood by those skilled in the art. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0054] In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly indicating the number of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include one or more of such features. In the description of the present invention, "a plurality" means two or more unless otherwise specifically defined.

[0055] All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present invention without making creative efforts fall within the scope of protection of the present invention.

Claims

1. A method for preparing a skin-care powder tetrandleaf extract, characterized in that: The following steps are involved: S1. Crush and sieve the cleaned and dried Radix Stephaniae Tetrandrae medicinal materials; S2, taking the coarse powder of Stephania tetrandra, adding an acidic aqueous solution with a pH value of 3.5 to 4.5, placing it in an extraction device for dual extraction by microfluidics and ultrasound, and separating the clear liquid after the extraction is completed; S3, take the above clear liquid, directly adsorb it through the pretreated macroporous cation exchange resin D72, rinse the macroporous cation exchange resin D72 with an equal amount of distilled water after adsorption, and then elute the adsorption saturated resin column with 0.4-0.5 mol / L ammonia ethanol solution at a flow rate of 10 mL / min until the eluent has no alkaloid reaction; S4. Concentrate the eluate, then dissolve it with a supersaturated ethanol solution, and repeat recrystallization twice to obtain high-purity white powdered tetrandrine.

2. The method for preparing the skin-care powder Tetrandrakia tetrandra extract according to claim 1, characterized in that: In step S2, the material ratio of the coarse powder of Stephania tetrandra to the acidic aqueous solution is 1:5 to 1:

7.

3. The method for preparing the skin-care powder Tetrandrakia tetrandra extract according to claim 1, characterized in that: In step S2, the power of the ultrasonic wave is 500W, and the extraction time is 60 to 90 minutes.

4. The method for preparing the skin-care powder Tetrandrakia tetrandra extract according to claim 1, characterized in that: The pretreatment method of the macroporous cation exchange resin D72 is as follows: soak the macroporous cation exchange resin D72 in anhydrous ethanol for 24 hours, and change the anhydrous ethanol solution every 3 hours, and stir continuously to make the ethanol solution fully contact with the macroporous cation exchange resin D72 and drive away the air. After complete soaking, rinse with deionized water until there is no ethanol smell, then soak with 5% hydrochloric acid for 3 to 4 hours, then rinse with deionized water until neutral, then soak with 5% sodium hydroxide solution for 3 to 4 hours, then rinse with deionized water until neutral, and then place in a drying oven to dry for use.

5. An extraction device as claimed in claim 1, characterized in that: It comprises a mixing part (1), an extraction part (2) and a centrifugal part (3) which are connected in sequence; The mixing section (1) comprises a mixing tank (101), the upper end of the mixing tank (101) is provided with a feeding port (1011), the lower end of the mixing tank (101) is provided with a discharge port (1012), the discharge port (1012) is provided with a valve (1013) and the discharge port (1012) is connected to a feeding pipe (102), and a stirring mechanism (103) is provided inside the mixing tank (101); The extraction part (2) comprises an extraction tank (201), the first feeding pipe (102) is connected to the side wall of the extraction tank (201), the lower end of the extraction tank (201) is provided with a second discharge port (2011), the second discharge port (2011) is provided with a second valve (2012) and the second discharge port (2011) is connected to a second feeding pipe (202), the second feeding pipe (202) is connected to a delivery pump (203), and the extraction tank (201) is connected to a microjet mechanism (204) and an ultrasonic mechanism (205); The centrifugal part (3) comprises a frame (301), a rotating centrifugal tank (302) is mounted on the frame (301), a ring gear (3025) is fixedly mounted on the outer wall of the centrifugal tank (302), a motor (303) is mounted on the frame (301), a gear (3031) meshingly connected with the ring gear (3025) is disposed at the output end of the motor (303), a discharge port (3021) is disposed at the lower end of the centrifugal tank (302), a valve (3022) is disposed at the discharge port (3021), and the upper end of the centrifugal tank (302) is The centrifugal tank (302) is provided with a through hole (3023) at the center of the upper end thereof, and a material injection pipe (304) and a liquid extraction pipe (305) are inserted into the through hole (3023). The material injection pipe (304) is fixed on the frame (301), and the upper end of the material injection pipe (304) is connected to the second feeding pipe (202). The liquid extraction pipe (305) is provided on the frame (301) through a lifting mechanism, and the upper end of the liquid extraction pipe (305) is connected to the third feeding pipe (306), and the third feeding pipe (306) is connected to the second delivery pump (307).

6. The extraction device according to claim 5, characterized in that The stirring mechanism (103) comprises an upper support (1031) and a lower support (1032) which are arranged in the mixing tank (101); the upper support (1031) is arranged close to the upper end of the mixing tank (101); the lower support (1032) is arranged close to the lower end of the mixing tank (101); a vertically arranged guide circular tube (1033) is connected between the upper support (1031) and the lower support (1032); the upper and lower ends of the guide circular tube (1033) are through-connected; two symmetrically arranged guide slideways (1034) are arranged on the side wall of the guide circular tube (1033); the guide slideways (1034) are arranged in a vertical direction; and the guide slideways (1034) are arranged in a vertical direction. 34) is wound around the guide circular tube (1033) along the length direction of the guide circular tube (1033), a sliding driving block (1035) is matched inside the guide circular tube (1033), two stirring rods (1036) are fixedly provided on both sides of the driving block (1035) and extend out of the guide circular tube (1033) through two guide slideways (1034), and a vertically arranged cylinder 1 (1037) is installed at the top of the mixing tank (101), and the telescopic rod of the cylinder 1 (1037) extends downward into the guide circular tube (1033) and is rotatably connected to the center of the driving block (1035).

7. The extraction device according to claim 5, characterized in that The microfluidic mechanism (204) comprises a nozzle (2041) fixedly arranged on the side wall of the extraction tank (201); the nozzle (2041) is arranged close to the upper end of the extraction tank (201); a horizontally connected high-pressure air channel (2042) and a compression channel (2043) are arranged in the nozzle (2041); the high-pressure air channel (2042) extends outwardly and is connected to a connection port (2044) arranged at the outer end of the nozzle (2041); the compression channel (2043) is arranged toward the extraction tank (201); An injection port (2045) is extended and connected to the inner wall of the extraction tank (201); the connection port (2044) is connected to a gas tank (2048) via an air supply pipe (2049); a high-pressure pump is connected to the air supply pipe (2049); a feed port (2046) is provided at the upper end of the nozzle (2041); the feed port (2046) is vertically connected to the compression channel (2043) via a vertically arranged feed channel (2047); and the feed port (2046) is connected to feed pipe 1.

8. The extraction device according to claim 5, characterized in that The ultrasonic mechanism (205) comprises an ultrasonic controller (2051), an ultrasonic transducer (2052), an ultrasonic amplitude transformer (2053) and a tool head (2054); the ultrasonic transducer (2052) is fixedly mounted on the upper end of the extraction tank (201); the tool head (2054) is located in the extraction tank (201); the ultrasonic transducer (2052) is connected to the tool head (2054) via the ultrasonic amplitude transformer (2053); and the ultrasonic transducer (2052) is connected to the ultrasonic controller (2051) via electric wires.

9. The extraction device according to claim 5, characterized in that An annular rotating edge (3024) is fixedly connected to the outer wall of the centrifugal tank (302), the center of the rotating edge (3024) is located on the rotation axis of the centrifugal tank (302), and an annular rotating groove (3011) is opened on the frame (301), the rotating groove (3011) matches the rotating edge (3024), and the rotating edge (3024) is embedded in the rotating groove (3011) and slidably arranged.

10. The extraction device according to claim 5, characterized in that The lifting mechanism comprises a second cylinder (308) vertically mounted on a frame (301), a telescopic rod of the second cylinder (308) being arranged downward, and the liquid extraction pipe (305) being mounted on the telescopic rod of the second cylinder (308) via a connecting frame.

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