Special fertilizer for promoting growth of root nodules of albizia julibrissin and preparation method of special fertilizer

Abstract

The invention discloses a special fertilizer for promoting growth of root nodules of albizia julibrissin and a preparation method thereof, the special fertilizer comprises: a pellet core comprising a phosphate fertilizer, an organic matter and a root nodule promoting substance, the pellet core comprises, by percentage, 37-42% of the phosphate fertilizer, 50-60% of the organic matter and 3-8% of the root nodule promoting substance, and the root nodule promoting substance comprises triacontanol, genistein and chitin; the pellet core is coated with a microbial agent for promoting the growth of the albizia julibrissin root nodule to form a microbial agent layer, and the colony count of the microbial agent is greater than or equal to 4 * 10 < 9 > CFU / mL; a film-forming agent is applied to the fungicide layer, a fungicide protection layer is formed on the fungicide layer, and the film-forming agent comprises one or more of a polyvinyl alcohol solution, a polyacrylic acid solution and a chitin solution. Compared with the prior art, by applying the special fertilizer, the number and average weight of root nodules of the albizia julibrissin are increased, meanwhile, sufficient phosphorus and carbon are provided for young forest of the albizia julibrissin, and the growth amount of the albizia julibrissin is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of fertilizers for forest trees, and particularly relates to a special fertilizer for promoting the growth of Albizia odoratissima root nodules and a preparation method thereof. Background Art

[0002] Albizia odoratissima (Linn.f.) Benth, also known as Albizia odoratissima, Heige, Xiangxushu, and Tugemu, is a plant of the genus Albizia in the family Fabaceae. The roots of Albizia odoratissima are highly sensitive to prokaryotic nitrogen-fixing microorganisms, causing the prokaryotic nitrogen-fixing microorganisms to invade its roots and stimulate the proliferation of root cells to form nodules, that is, root nodules. The root nodules formed by the roots of Albizia odoratissima can fix a large amount of nitrogen in the soil to meet its own nutritional needs and improve the nitrogen level of the soil.

[0003] The application of fertilizer components and the number and growth of Albizia odoratissima root nodules affect and limit the vegetative growth of Albizia odoratissima. Currently, there are few records on fertilizers related to promoting root nodule growth, and further research on special fertilizers for promoting the growth of Albizia odoratissima root nodules is blank.

[0004] CN114790123A discloses a fertilizer for promoting root nodule growth, its preparation method and application, specifically discloses that the carbon-nitrogen ratio of the fertilizer is 18.6 - 22.7, and the nitrogen-phosphorus ratio is 6.5 - 7.1; the preparation method of the fertilizer is to mix dry decomposed cow dung with water, stir and leach with oxygen for 24 - 28 hours, and filter and dry through a 100-mesh sieve to obtain the fertilizer; by mass ratio, the ratio of the dry decomposed cow dung to water is 1:6. It can improve the infection efficiency of rhizobia on leguminous plants and promote root nodule formation. However, this method requires leaching of decomposed cow dung to optimize its available nutrient composition, which is not only troublesome for secondary treatment of decomposed cow dung, but also the nitrogen-phosphorus ratio of this fertilizer is relatively high, while the total phosphorus in the soil of Guangxi is relatively low, and young Albizia odoratissima forests have a large demand for phosphorus and require more phosphorus elements during growth. Therefore, this fertilizer is not suitable for Albizia odoratissima.

[0005] In order to promote the formation of Albizia odoratissima root nodules and the healthy growth of Albizia odoratissima forest trees, it is necessary to prepare a special fertilizer suitable for and promoting the growth of Albizia odoratissima root nodules according to the characteristics of Albizia odoratissima root nodule formation and the root system development characteristics of Albizia odoratissima, and formulate a corresponding fertilization plan according to the fertilizer characteristics. Summary of the Invention

[0006] The purpose of the present invention is to provide a special fertilizer for promoting the growth of Albizia odoratissima root nodules, aiming to solve the problems of fewer Albizia odoratissima root nodules, poor activity, easy shrinkage, and the existing fertilizers being unsuitable for the formation of Albizia odoratissima root nodules and the growth of Albizia odoratissima.

[0007] The purpose of the present invention is to provide a preparation method of a special fertilizer for promoting the growth of Albizia odoratissima root nodules, aiming to achieve the effect of promoting the growth of Albizia odoratissima root nodules by the special fertilizer.

[0008] To achieve the above object, the present invention provides a special fertilizer for promoting the nodule growth of Albizia odoratissima, and the special fertilizer includes:

[0009] The core of the pellet is phosphate fertilizer, organic matter and nodule-promoting substances. The core of the pellet is 37-42% phosphate fertilizer, 50-60% organic matter and 3-8% nodule-promoting substances by percentage. The nodule-promoting substances include triacontanol, genistein and chitin;

[0010] The core of the pellet is coated with a microbial inoculant for promoting the nodule growth of Albizia odoratissima to form an inoculant layer, and the colony count of the microbial inoculant ≥ 4×10 9 CFU / mL;

[0011] A film-forming agent is applied to the inoculant layer to form an inoculant protection layer on the inoculant layer. The film-forming agent includes one or more of polyvinyl alcohol solution, polyacrylic acid solution and chitin solution.

[0012] Preferably, in the above technical solution, in the process of coating the core of the pellet with the microbial inoculant for promoting the nodule growth of Albizia odoratissima to form an inoculant layer, the microbial inoculant is Rhizobium tropici with a colony count ≥ 4×10 9 CFU / mL.

[0013] Preferably, in the above technical solution, among the nodule-promoting substances including triacontanol, genistein and chitin, the weight ratio of triacontanol, genistein and chitin is 5-9:2-3.5:10-15.

[0014] Preferably, in the above technical solution, the nodule-promoting substances further include nerolidol and myristic acid.

[0015] Preferably, in the above technical solution, triacontanol, genistein, chitin, nerolidol and myristic acid in the nodule-promoting substances are prepared according to a weight ratio of 5-9:2-3.5:10-15:1-2:2-4.

[0016] Preferably, in the above technical solution, the film-forming agent is a 5-10% polyvinyl alcohol solution or / and a 5-10% polyacrylic acid solution or / and a 50-60% chitin solution.

[0017] A preparation method of a special fertilizer for promoting the nodule growth of Albizia odoratissima, and the preparation method includes:

[0018] S1, preparing a nodule-promoting agent: weighing nodule-promoting substances, mixing the nodule-promoting substances and polyvinyl alcohol solution according to a preset ratio, standing at room temperature, and drying in vacuum to obtain a nodule-promoting agent;

[0019] S2. Prepare the microbial inoculant: Activate the microbial powder to obtain a microbial inoculant with the number of colonies in 1 mL of the bacterial liquid ≥ 4×10 9 CFU;

[0020] S3. Mix the nodulation promoter, organic matter, and phosphate fertilizer to obtain a mixture. Add the mixture to a granulator to make the core. Atomize the microbial inoculant through a spray dryer and spray it onto the surface of the core to form a bacterial agent layer. Dry it at room temperature. Atomize the film-forming agent through a spray dryer and spray it onto the surface of the bacterial agent layer. Dry it at room temperature to form a protective layer for the bacterial agent.

[0021] Preferably, in the above technical solution, in step S1, the specific steps for preparing the nodulation promoter are as follows:

[0022] S11. Weigh the nodulation-promoting substances, which include several of triacontanol, genistein, chitin, nerolidol, and myristic acid;

[0023] S12. Mix the nodulation-promoting substances and the polyvinyl alcohol solution in a mass ratio of 1-2:1, let it stand at room temperature for 10-15 min, and dry it under vacuum to obtain the nodulation promoter.

[0024] Preferably, in the above technical solution, the nodulation-promoting substances are triacontanol, genistein, and chitin in a weight ratio of 5-9:2-3.5:10-15;

[0025] Or the nodulation-promoting substances are triacontanol, genistein, chitin, nerolidol, and myristic acid in a weight ratio of 5-9:2-3.5:10-15:1-2:2-4.

[0026] Compared with the prior art, the present application has the following beneficial effects:

[0027] (1) The special fertilizer of the present invention has the effects of promoting the increase in the number of root nodules of Albizia odoratissima and reducing the shrinkage of root nodules of Albizia odoratissima. The principle of promoting the growth of root nodules of Albizia odoratissima is as follows: First, use tropical rhizobia as the specific microbial inoculant. The nodulation factors of tropical rhizobia can be recognized by the receptors on the surface of the root hair cells of Albizia odoratissima, triggering the formation of nodule primordia on the root hairs of Albizia odoratissima and gradually developing into root nodules. At the same time, tropical rhizobia infect the developing root nodules. Second, myristic acid, chitin, and genistein act together to stimulate tropical rhizobia to synthesize and secrete nodulation factors, improving the infection efficiency of tropical rhizobia. At the same time, triacontanol improves the root activity of Albizia odoratissima, promoting the root system of Albizia odoratissima to receive and recognize nodulation factors, jointly realizing the nodulation and nitrogen fixation of the root system of Albizia odoratissima and tropical rhizobia. Finally, the nodulation promoter, phosphate fertilizer, and humic acid are released, providing nitrogen source, carbon source, and energy for the growth and reproduction of tropical rhizobia, increasing the root nodule content in the soil around the treatment object.

[0028] (2) The special fertilizer of the present invention can improve the limitation of the low total phosphorus content in the soil of the Albizia odoratissima forest on the growth of Albizia odoratissima and promote the stable growth of Albizia odoratissima trees. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a diagram of the root system of Albizia odoratissima with root nodules in a specific embodiment of the present invention;

[0030] Figure 2 It is a diagram of the obvious growth of root nodules of Albizia odoratissima in a specific embodiment of the present invention;

[0031] Figure 3 It is a diagram of the influence of the number of root nodules of Albizia odoratissima on plant height in a specific embodiment of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0032] The technical solutions in the embodiments of the present invention are clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0033] Biological source:

[0034] Tropical rhizobia were purchased from Beijing BioWin Biotech Co., Ltd.

[0035] Example 1

[0036] A preparation method of a special fertilizer for promoting the growth of root nodules of Albizia odoratissima, the method is as follows:

[0037] S1. Prepare a nodulation promoter, including:

[0038] S11. Weigh 4.8 kg of triacontanol powder, 1.8 kg of genistein powder, 6 kg of chitin powder, 1.2 kg of nerolidol powder and 1.8 kg of myristic acid powder, mix them evenly to obtain nodulation-promoting substances;

[0039] S12. Mix the nodulation-promoting substances with a polyvinyl alcohol solution in a mass ratio of 1:1, let it stand at room temperature for 15 minutes, and dry it in vacuum to obtain a nodulation promoter.

[0040] S2. Prepare a microbial inoculant:

[0041] S21. Prepare a liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0042] S22, Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21. Cultivate it in a constant-temperature shaker for 2 days, then inoculate it into the liquid medium in step S21 and cultivate it in a constant-temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain the microbial inoculant for preparing the inoculant layer;

[0043] S3, Mix 8% nodulation promoter, 50% organic matter, and 42% phosphate fertilizer evenly, add them to a granulator to make granular cores for the special fertilizer; atomize the microbial inoculant through a spray dryer and spray it onto the surface of the cores, then dry it at room temperature. Repeat this step 2 times to form an inoculant layer; atomize a 5% polyvinyl alcohol solution through a spray dryer and spray it onto the surface of the inoculant layer, then dry it at room temperature. Repeat this step 1 time to form an inoculant protection layer. Among them, the phosphate fertilizer is calcium dihydrogen phosphate, and the organic matter is humic acid.

[0044] Example 2

[0045] A preparation method of a special fertilizer for promoting the growth of Acacia concinna root nodules, the method is as follows:

[0046] S1, Prepare the nodulation promoter, including:

[0047] S11, Weigh 4.0 kg of triacontanol powder, 1.25 kg of genistein powder, 5 kg of chitin powder, 1 kg of nerolidol powder, and 1 kg of myristic acid powder, mix them evenly to obtain the nodulation-promoting substance;

[0048] S12, Mix the nodulation-promoting substance and the polyvinyl alcohol solution evenly at a mass ratio of 1:1, let it stand at room temperature for 15 min, and dry it under vacuum to obtain the nodulation promoter.

[0049] S2, Prepare the microbial inoculant:

[0050] S21, Prepare the liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine, and 5000 mL of water, and adjust the pH value to 7.2;

[0051] S22, Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21. Cultivate it in a constant-temperature shaker for 1.5 days, then inoculate it into the liquid medium in step S21 and cultivate it in a constant-temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain the microbial inoculant for preparing the inoculant layer;

[0052] S3. Mix 5% root nodule promoter, 60% organic matter and 35% phosphate fertilizer, add them into a granulator, and make them into granular cores for the special fertilizer; atomize the microbial inoculant through a spray dryer, spray it onto the surface of the cores, and dry it at room temperature. Repeat this step 2 times to form an inoculant layer; atomize a 5% polyvinyl alcohol solution through a spray dryer, spray it onto the surface of the inoculant layer, and dry it at room temperature. Repeat this step 1 time to form an inoculant protection layer. Among them, the phosphate fertilizer is calcium dihydrogen phosphate, and the organic matter is humic acid.

[0053] Example 3

[0054] A preparation method of a special fertilizer for promoting the root nodule growth of Albizia odoratissima, the method is specifically as follows:

[0055] S1. Prepare a root nodule promoter, including:

[0056] S11. Weigh 2.5 kg of triacontanol powder, 1 kg of genistein powder, 6 kg of chitin powder, 0.5 kg of nerolidol powder and 1 kg of myristic acid powder, mix them evenly to obtain root nodule promoting substances;

[0057] S12. Mix the root nodule promoting substances with a polyvinyl alcohol solution at a mass ratio of 1:1, let it stand at room temperature for 10 min, and dry it under vacuum to obtain a root nodule promoter.

[0058] S2. Prepare a microbial inoculant:

[0059] S21. Prepare a liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0060] S22. Weigh 1 g of tropical rhizobium powder and add it to the liquid medium in step S21, culture it in a constant temperature shaker for 2 days, then inoculate it into the liquid medium in step S21, and culture it in a constant temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain a microbial inoculant for preparing an inoculant layer;

[0061] S3. Mix 6% root nodule promoter, 56% organic matter and 38% phosphate fertilizer to obtain a mixture, add the mixture into a granulator to make cores, atomize the microbial inoculant through a spray dryer, spray it onto the surface of the cores, and dry it at room temperature. Repeat this step 2 times to form an inoculant layer; atomize a 5% polyvinyl alcohol solution through a spray dryer, spray it onto the surface of the inoculant layer, and dry it at room temperature. Repeat this step 1 time to form an inoculant protection layer. Among them, the phosphate fertilizer is calcium dihydrogen phosphate, and the organic matter is humic acid.

[0062] Example 4

[0063] A preparation method of a special fertilizer for promoting the growth of Albizia odoratissima root nodules, the method is specifically as follows:

[0064] S1, Prepare a root nodule promoter, including:

[0065] S11, Weigh 3.5 kg of triacontanol powder, 1.5 kg of genistein powder, 6 kg of chitin powder, 1 kg of nerolidol powder and 1 kg of myristic acid powder, mix them evenly to obtain a root nodule promoting substance;

[0066] S12, Mix the root nodule promoting substance and polyvinyl alcohol solution in a mass ratio of 1:1, let it stand at room temperature for 10 min, and dry it in vacuum to obtain a root nodule promoter.

[0067] S2, Prepare a microbial inoculant:

[0068] S21, Prepare a liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0069] S22, Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21, culture it in a constant temperature shaker for 2 days, then inoculate it into the liquid medium in step S21, and culture it in a constant temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU, to obtain a microbial inoculant for preparing the inoculant layer;

[0070] S3, Mix 8% of the root nodule promoter, 50% of the organic matter and 42% of the phosphate fertilizer to obtain a mixture, add the mixture to a granulator to make a core, atomize the microbial inoculant through a spray dryer, spray it onto the surface of the core, and dry it at room temperature. This step is repeated 2 times to form an inoculant layer; Atomize 5% of the polyvinyl alcohol solution through a spray dryer, spray it onto the surface of the inoculant layer, and dry it at room temperature. This step is repeated 1 time to form an inoculant protective layer, where the phosphate fertilizer is calcium dihydrogen phosphate and the organic matter is humic acid.

[0071] Comparative Example 1

[0072] A preparation method of a special fertilizer for promoting the growth of Albizia odoratissima root nodules, the method is specifically as follows:

[0073] S1, Prepare a microbial inoculant:

[0074] S11, Prepare the liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0075] S12, Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21. Cultivate it in a constant temperature shaker for 2 days, then inoculate it into the liquid medium in step S21 and cultivate it in a constant temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain the microbial inoculum for preparing the inoculum layer;

[0076] S2, Mix 58% of organic matter and 42% of phosphate fertilizer, add them to a granulator to make granular cores for the special fertilizer; Atomize the microbial inoculum through a spray dryer and spray it onto the surface of the cores, and dry it at room temperature. Repeat this step 2 times to form the inoculum layer; Atomize a 5% polyvinyl alcohol solution through a spray dryer and spray it onto the surface of the inoculum layer, and dry it at room temperature. Repeat this step 1 time to form the inoculum protection layer, where the phosphate fertilizer is calcium dihydrogen phosphate and the organic matter is humic acid.

[0077] Comparative Example 2

[0078] A preparation method of a special fertilizer for promoting the nodule growth of Albizia odoratissima, the method is as follows:

[0079] S1, Prepare the microbial inoculum:

[0080] S11, Prepare the liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0081] S12, Weigh 1 g of soybean rhizobia powder and add it to the liquid medium in step S21. Cultivate it in a constant temperature shaker for 2 days, then inoculate it into the liquid medium in step S21 and cultivate it in a constant temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain the microbial inoculum for preparing the inoculum layer;

[0082] S2, Mix 58% of organic matter and 42% of phosphate fertilizer, add them to a granulator to make granular cores for the special fertilizer; Atomize the microbial inoculum through a spray dryer and spray it onto the surface of the cores, and dry it at room temperature. Repeat this step 2 times to form the inoculum layer; Atomize a 5% polyvinyl alcohol solution through a spray dryer and spray it onto the surface of the inoculum layer, and dry it at room temperature. Repeat this step 1 time to form the inoculum protection layer, where the phosphate fertilizer is calcium dihydrogen phosphate and the organic matter is humic acid.

[0083] Comparative Example 3

[0084] A preparation method of a special fertilizer for promoting the growth of Albizia odoratissima root nodules, the method is as follows:

[0085] S1, Prepare a root nodule promoting agent, including:

[0086] S11, Weigh 1.8 kg of genistein powder, 6 kg of chitin powder and 1.2 kg of nerolidol powder, mix them evenly to obtain a root nodule promoting substance;

[0087] S12, Mix the root nodule promoting substance with a polyvinyl alcohol solution in a mass ratio of 1:1, let it stand at room temperature for 15 min, and dry it in vacuum to obtain a root nodule promoting agent.

[0088] S2, Prepare a microbial inoculant:

[0089] S21, Prepare a liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2;

[0090] S22, Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21, culture it in a constant temperature shaker for 2 days, then inoculate it into the liquid medium in step S21, and culture it in a constant temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU, to obtain a microbial inoculant for preparing the inoculant layer;

[0091] S3, Mix 8% of the root nodule promoting agent, 50% of the organic matter and 42% of the phosphate fertilizer evenly, add them to a granulator to make granular cores for the special fertilizer; Atomize the microbial inoculant through a spray dryer and spray it onto the surface of the cores, and dry it at room temperature. This step is repeated 2 times to form an inoculant layer; Atomize a 5% polyvinyl alcohol solution through a spray dryer and spray it onto the surface of the inoculant layer, and dry it at room temperature. This step is repeated 1 time to form an inoculant protection layer, where the phosphate fertilizer is calcium dihydrogen phosphate and the organic matter is humic acid.

[0092] Comparative Example 4

[0093] A preparation method of a special fertilizer for promoting the growth of Albizia odoratissima root nodules, the method is as follows:

[0094] S1, Prepare a root nodule promoting agent, including:

[0095] S11, Weigh 1.8 kg of genistein powder and 6 kg of chitin powder, mix them evenly to obtain a root nodule promoting substance;

[0096] S12. Mix the nodule-promoting substance and the polyvinyl alcohol solution in a mass ratio of 1:1, let it stand at room temperature for 15 min, and then dry it under vacuum to obtain the nodule-promoting agent.

[0097] S2. Prepare the microbial inoculant:

[0098] S21. Prepare the liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2.

[0099] S22. Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21. Culture it in a constant-temperature shaker for 2 days, then inoculate it into the liquid medium in step S21 again and culture it in a constant-temperature shaker until the number of colonies in 1 mL of the bacterial solution is ≥ 4×10 9 CFU to obtain the microbial inoculant for preparing the inoculant layer.

[0100] S3. Mix 8% of the nodule-promoting agent, 50% of the organic matter and 42% of the phosphate fertilizer, add them to a granulator to make granular cores for the special fertilizer. Atomize the microbial inoculant with a spray dryer and spray it onto the surface of the cores, and dry it at room temperature. Repeat this step 2 times to form the inoculant layer. Atomize 5% of the polyvinyl alcohol solution with a spray dryer and spray it onto the surface of the inoculant layer, and dry it at room temperature. Repeat this step 1 time to form the inoculant protection layer. Among them, the phosphate fertilizer is calcium dihydrogen phosphate, and the organic matter is humic acid.

[0101] Comparative Example 5

[0102] It is basically the same as Example 1, the difference is that the preparation methods are different.

[0103] A preparation method of a special fertilizer for promoting the nodule growth of Albizia odoratissima, the method is as follows:

[0104] S1. Prepare the nodule-promoting agent, including:

[0105] S11. Weigh 2.5 kg of triacontanol powder, 1 kg of genistein powder, 5 kg of chitin powder, 0.5 kg of nerolidol powder and 1 kg of myristic acid powder, and mix them to obtain the nodule-promoting substance.

[0106] S2. Prepare the microbial inoculant:

[0107] S21. Prepare the liquid medium: Take 5.0 g of yeast extract, 50.0 g of mannitol, 2.5 g of K2HPO4, 1 g of MgSO4, 0.5 g of NaCl, 0.5 g of CaCl2, 0.5 g of betaine and 5000 mL of water, and adjust the pH value to 7.2.

[0108] S22. Weigh 1 g of tropical rhizobia powder and add it to the liquid medium in step S21. Cultivate it in a constant-temperature shaker for 2 days, then inoculate it into the liquid medium in step S21 and cultivate it in a constant-temperature shaker until the number of colonies in 1 mL of the bacterial solution ≥ 4×10 9 CFU to obtain the microbial inoculum for preparing the inoculum layer;

[0109] S3. Mix 8% of the root nodule-promoting substance, 50% of the organic matter, and 42% of the phosphate fertilizer, add them to a granulator to make granular cores for the special fertilizer; atomize a 5% polyvinyl alcohol solution through a spray dryer and dry it at room temperature, repeat this step once to form an isolation film; atomize the microbial inoculum through a spray dryer and spray it onto the surface of the core, dry it at room temperature, repeat this step twice to form an inoculum layer; atomize a 5% polyvinyl alcohol solution through a spray dryer and spray it onto the surface of the inoculum layer, dry it at room temperature, repeat this step once to form an inoculum protection layer. Among them, the phosphate fertilizer is calcium dihydrogen phosphate, and the organic matter is humic acid.

[0110] When applying the base fertilizer in March - April in spring, fertilize the Albizia odoratissima with a tree age of 2 - 3 years. Open a fertilization trench with a size of 0.1 m × 0.5 m on one side at a distance of 1.3 - 1.8 m from the trunk of the Albizia odoratissima tree. During the process of opening the fertilization trench, collect the residual roots and take part of the soil from the trench soil as experimental soil. After applying 1 kg of the special fertilizer in the fertilization trench, cover the soil. Bring the experimental soil and the residual roots back to the laboratory, count the number of root nodules on the residual roots and weigh them, calculate the average weight of each root nodule, and repeat the above fertilization method during the next year's fertilization. The special fertilizer can be appropriately increased according to the soil conditions, and other fertilizers can also be added.

[0111] Among them, the root nodule situation of Albizia odoratissima before and after applying the special fertilizer is shown in Table 1.

[0112] Table 1 Total number and average weight of root nodules of Albizia odoratissima before and after applying the special fertilizer

[0113]

[0114] As can be seen from Table 1 above, after the treatments of Examples 1 - 4, the number of root nodules increased significantly, and the average weight of root nodules also increased significantly. After the treatments of Comparative Examples 1 - 5, the number of root nodules also increased to some extent, but the effect was not as good as that of Example 1. Among them, in Comparative Example 5, it may be that the humic acid directly contacted the root nodule-promoting substance, resulting in a decrease in the activity of the root nodule-promoting substance, and thus the number and average weight of root nodules were worse than those of Example 1.

[0115] Take the experimental soil to detect the physical and chemical properties of the soil. The detection items include total organic carbon (TOC), total nitrogen (TN), and total phosphorus (TP). Specifically, the TOC is determined by the sulfuric acid - potassium dichromate oxidation method, the TN is determined by the Kjeldahl method, and the TP is determined by the molybdenum antimony colorimetric method. The results are shown in Table 2.

[0116] Table 2 Soil Physical and Chemical Properties Table

[0117]

[0118]

[0119] Calculate C:P and N:P based on the above TOC, TN, and TP data, and the results are shown in Table 3

[0120] Table 3 C:P and N:P Conditions before and after Applying Special Fertilizer

[0121]

[0122] It can be seen from Table 3 that the soil C:P and N:P after treatment may be related to root nodules and fertilizer nutrients

[0123] The present invention can be implemented in various different ways and is not limited to the described embodiments. Those of ordinary skill in the art can understand that the present invention can be implemented by other specific means without changing the technical idea or essential features of the present invention. Therefore, it should be understood that the above-described embodiments are exemplary and not intended to limit the present invention

Claims

1. A special fertilizer for promoting the growth of Albizia albizzia root nodules, characterized in that: The special fertilizer comprises: The pellet core is phosphate fertilizer, organic matter and root nodule promoting substances, wherein the pellet core is 37-42% phosphate fertilizer, 50-60% organic matter and 3-8% root nodule promoting substances in percentage, and the root nodule promoting substances include triacontanol, genistein and chitin; The microbial agent for promoting the growth of root nodules of Albizzia julibrissin is coated on the pellet core to form a microbial agent layer, and the colony number of the microbial agent is ≥4×10 9 CFU / mL; The film-forming agent is applied on the bacterial agent layer to form a bacterial agent protective layer on the bacterial agent layer. The film-forming agent includes one or more of polyvinyl alcohol solution, polyacrylic acid solution and chitin solution.

2. The special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 1, characterized in that: In the microbial agent coating the ball core to form a microbial agent layer, the microbial agent has a colony count of ≥4×10 9 CFU / mL of tropical rhizobium.

3. The special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 1, characterized in that: The nodule-promoting substances include triacontanol, genistein and chitin, and the weight ratio of triacontanol, genistein and chitin is 5-9:2-3.5:10-15.

4. The special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 1, characterized in that: The nodule promoting substances also include nerolidol and myristic acid.

5. The special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 3, characterized in that: The triacontanol, genistein, chitin, nerolidol and myristic acid in the nodule-promoting substance are prepared in a weight ratio of 5-9:2-3.5:10-15:1-2:2-4.

6. The special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 1, characterized in that: The film-forming agent is 5-10% polyvinyl alcohol solution or / and 5-10% polyacrylic acid solution or / and 50-60% chitin solution.

7. A method for preparing a special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in any one of claims 1 to 5, characterized in that: The preparation method comprises: S1, preparing a nodule-promoting agent: weighing a nodule-promoting substance, mixing the nodule-promoting substance with a polyvinyl alcohol solution in a preset ratio, standing the mixture at room temperature, and vacuum drying the mixture to obtain a nodule-promoting agent; S2, preparation of microbial inoculants: activation of microbial powder to obtain a colony count of ≥ 4 × 10 in 1 mL of bacterial solution 9 CFU of microbial agents; S3, mixing the nodule promoter, organic matter and phosphate fertilizer to obtain a mixture, adding the mixture into a granulator to make a pellet core, atomizing the microbial agent through a spray dryer, spraying it on the surface of the pellet core to form a microbial agent layer, drying at room temperature, atomizing the film-forming agent through a spray dryer, spraying it on the surface of the microbial agent layer, drying at room temperature to form a microbial agent protective layer.

8. The method for preparing the special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 7, characterized in that: In step S1, the specific steps of preparing the nodule promoting agent are as follows: S11, weighing root nodule promoting substances, wherein the root nodule promoting substances include several of triacontanol, genistein, chitin, nerolidol and myristic acid; S12, mixing the nodule-promoting substance and the polyvinyl alcohol solution at a mass ratio of 1-2:1, standing at room temperature for 10-15 minutes, and vacuum drying to obtain a nodule-promoting agent.

9. The method for preparing the special fertilizer for promoting the growth of Albizia albizzia root nodules as claimed in claim 8, characterized in that: The nodule-promoting substance is triacontanol, genistein, and chitin in a weight ratio of 5-9:2-3.5:10-15; Or the nodule-promoting substances are triacontanol, genistein, chitin, nerolidol and myristic acid in a weight ratio of 5-9:2-3.5:10-15:1-2:2-4.

Images