One for outdoor use 13 Convenient device and method for C isotope labeling

Abstract

The present invention relates to the technical field of isotope tracing technology and discloses a method for field 13 A convenient device and method for C isotope labeling, wherein the convenient device for labeling includes a cubic bracket, which is assembled from multiple horizontal tubes and multiple vertical tubes, and is covered with a plastic film. The cubic bracket and the plastic film are assembled into an isotope labeling box, the interior of the cubic bracket is connected, and an air inlet is opened on the wall of one of the vertical tubes, and a pressure edge assembly, which includes an internal pressure edge frame and an external pressure edge frame, the external pressure edge frame adopts a splicing design, and the internal pressure edge frame is sleeved on the cubic bracket. 13 The convenient device and method for C isotope labeling can be flexibly disassembled to form a labeling box to meet the transportation and movement needs in scenarios such as changing sample sites in the field. The size of the labeling box can also be flexibly adjusted in the field scene, making it suitable for in situ labeling experiments in various field ecosystems.

Description

Technical Field

[0001] The present invention relates to the field of isotope tracing technology, specifically a method for 13 Convenient device and method for C isotope labeling. Background Art

[0002] existing 13 C marking is mainly carried out through a marking box, which is usually made of transparent materials (such as polycarbonate) to ensure sufficient light. The box is sealed and equipped with a liquid filling port or air inlet and outlet to control the gas composition. Sensors are installed inside the box to monitor temperature, humidity and CO2 concentration. The marking principle is to inject 13 C-labeled CO2( 13 CO2) or inject concentrated sulfuric acid (H2SO4) and drip into the Na 13 CO3 reacts to produce 13 CO2(Na2 13 CO3+H2SO4→Na2SO4+H2O+ 13 CO2), plants absorb and fix these labeled carbons during photosynthesis. Subsequently, the collected plant, soil and gas samples were analyzed and determined by isotope ratio mass spectrometry (IRMS). 13 C / 12 Considering the portability and labeling effectiveness of field operations, most researchers now use concentrated sulfuric acid (H2SO4) to drip into the Na2CO3 in a pre-placed labeling box. 13 CO3 reacts to produce 13 CO2 labeling method, the existing 13 The C isotope labeling box is made of transparent acrylic board and other materials. It is divided into two parts: the base and the upper cover. The base is placed in the grassland sample to be marked in advance, and then the upper cover is placed and sealed with water to form a closed cubic space. 13 C isotope labeling operation.

[0003] At present, the existing transparent acrylic plate marking boxes are more suitable for indoor experiments. When used in the field, their surface is easily damaged during the marking process, affecting the light transmission effect. They have poor reusability and a relatively fixed volume. It is difficult to carry multiple marking boxes for isotope labeling at one time, and they are not suitable for changing multiple field sample sites. Summary of the Invention

[0004] (1) Technical problems solved

[0005] In view of the shortcomings of the prior art, the present invention provides a 13 The convenient device and method for C isotope labeling solve the problems of traditional labeling boxes being easily damaged during the labeling process when used in the field, having poor reusability, and having a relatively fixed volume, making it difficult to carry multiple labeling boxes for isotope labeling at one time, and not suitable for changing multiple field sample sites.

[0006] (2) Technical solution

[0007] In order to achieve the above-mentioned purpose, the present invention provides the following technical solutions: a method for 13 A convenient device for C isotope labeling includes a cubic support, the cubic support being assembled from a plurality of horizontal tubes and a plurality of vertical tubes, the cubic support being covered with a plastic film, the cubic support and the plastic film being assembled into an isotope labeling box, the interior of the cubic support being connected, and an air inlet being opened on the wall of one of the vertical tubes;

[0008] A side pressing assembly, comprising an inner side pressing frame and an outer side pressing frame, wherein the outer side pressing frame adopts a splicing design, and the inner side pressing frame is sleeved on the cubic bracket;

[0009] An isotope generator, which is mounted on a vertical side of the cubic support to facilitate the addition of chemicals and maintain the sealing of the interior of the isotope labeling box when the chemicals are injected;

[0010] An air circulation device is provided, wherein the air circulation component is installed on a cubic support at a position diagonal to the isotope generator, and the marking gas can be quickly mixed in the isotope marking box through the internally connected cubic support.

[0011] Preferably, a hemispherical sealing cover is provided at the upper end of the vertical tube, and a conical-structured opening is provided at the lower end of the vertical tube. Four threaded tubes are fixed on the tube wall of the vertical tube. The four threaded tubes are grouped in twos, and the center lines of the two threaded tubes in each group are perpendicular to each other. One group of the threaded tubes is vertically fixed at the sealing cover above the vertical tube, and the other group of the threaded tubes is vertically fixed on the tube wall of the vertical tube above the opening. Nuts are fixedly connected to the inner edges of the two tube openings of the horizontal tube, and the nuts cooperate with the tube wall of the threaded tube.

[0012] Preferably, multiple exhaust holes are opened on the tube walls of the multiple vertical tubes, and the multiple exhaust holes are all facing the vertical center line of the cube bracket. The air inlet opened on the tube wall of the vertical tube is used for the air flow transported by the air circulation device to enter and be discharged from the multiple exhaust holes.

[0013] Preferably, the internal pressing frame of the pressing assembly is made of welded steel bars, and the side length is two centimeters longer than the side length of the cube bracket. The external pressing frame is spliced ​​by two identical C-shaped bars, and the lower end of the C-shaped bar is fixedly connected to a pressure plate of the same structure, one of the C-shaped bars is fixedly connected to the pressure plate of the bar, and the other C-shaped bar is fixedly connected to two matching insertion strips on the pressure plate, and the fixed seat is connected with a hand-tightened bolt. Nail holes are provided at the four corners of the external pressing frame, and wedges are inserted into the nail holes.

[0014] Preferably, the isotope generating device includes a fixed frame, one side of the fixed frame is fixed on the tube wall of one of the vertical tubes, one side of the fixed frame is fixedly connected to a rectangular sleeve, a rectangular sleeve is sleeved in the rectangular sleeve, one side of the rectangular sleeve is fixedly connected to a sealing plate, a rubber seat is fixedly connected in the rectangular sleeve, a medicine storage tube is arranged in the rubber seat, the upper end of the rectangular sleeve is provided with a circular opening, a rubber plug is provided in the circular opening, the lower end of the rubber plug extends to the upper end tube opening of the medicine storage tube and is fixedly connected to a guide tube, the guide tube consists of an upper cone and a lower cone, a limiting flow hole is provided at the connection between the upper cone and the lower cone, the lower end of the lower cone is provided with a cone bottom, a plurality of liquid outlet holes are obliquely opened at the cone bottom, and an air outlet is provided on one side of the medicine storage tube.

[0015] Preferably, a rubber ring is fixedly connected to the air outlet, a right-angle elbow is sleeved inside the rubber ring, a clamping seat is provided on one side of the rubber seat, a gas cylinder is clamped in the clamping seat, the upper end of the gas cylinder is sealed with a bottle cap, one end of the right-angle elbow passes through the bottle cap and extends into the gas cylinder, and an air outlet pipe is installed on the other side of the bottle cap.

[0016] Preferably, one side of the sealing plate is fixedly connected to a magnetic sealing ring that can be attracted to the fixed frame, and a rubber ring is embedded in the inner edge of the pipe opening at one end of the rectangular sleeve. The rubber ring is a hollow structure and is sleeved with the side wall of the rectangular sleeve. A rectangular groove is provided on one side of the fixed frame.

[0017] Preferably, the air circulation device includes a bellows, one side of which is fixedly connected to a C-shaped snap ring, the C-shaped snap ring being clamped on the wall of the vertical tube and having an opening that matches the air inlet, a partition being fixedly connected inside the bellows, the partition dividing the bellows into a drying chamber and an air inlet chamber, one side of the bellows being fixedly connected to a No. 1 fan, the air outlet end of the No. 1 fan facing the air inlet and the opening;

[0018] A medicine placement port is provided at the upper end of the bellows, a sealing cover is installed at the medicine placement port, a No. 2 fan is installed at the upper end of the sealing cover, a plurality of air inlets are provided on the side wall of the bellows, a mesh plate is fixedly connected to each of the plurality of air inlets, two temperature and humidity sensors are installed at the air inlets, two positioning rings are fixedly connected to the tube wall of the vertical tube, and the positioning rings are each provided with a corresponding positioning protrusion that cooperates with the opening area of ​​the C-shaped clamping ring.

[0019] The present invention also provides a method for outdoor use. 13 C isotope labeling method, this method is suitable for field research in grassland, small trees, small shrubs, and wetland ecosystems. 13 The specific steps of the C isotope in situ labeling experiment are as follows:

[0020] Step 1: Make an internal cubic support by screwing multiple horizontal tubes onto the threaded tubes on multiple vertical tubes to form a cubic support, and install the isotope generator and air circulation device at the opposite corners;

[0021] Step 2: In the plot to be marked, select an area of ​​approximately one square meter. Place the cube bracket above the marked plant. Insert the vertical tube of the cube bracket into the soil using the tie-down until the bottom edge is flush with the ground. Once the cube bracket is fixed, leave a buffer zone of approximately 10 cm around the bottom edge to facilitate subsequent sealing with plastic film.

[0022] Step 3: Cover the cube support with a transparent plastic film, and use the internal pressing frame to smooth and compact the transparent plastic film along the top surface and vertical edges of the cube support from top to bottom;

[0023] Step 4: Insert the outer edge pressing frame, fix the four corners of the outer edge pressing frame to the ground with wedges, and then firmly fix the extended transparent plastic film to the ground to complete the assembly of the isotope labeling box;

[0024] Step 5: Use a syringe needle to quantitatively inject 5 ml of concentrated sulfuric acid into the drug storage tube in the isotope generator. The concentrated sulfuric acid and the Na2 13 CO3 reacts to produce 13 The marking process was completed by CO2 gas, and this step was repeated 3 times 1, 2, and 4 hours after the first marking.

[0025] Step 6: 6 hours after the first marking, pierce the transparent plastic film with a syringe, take 100 ml of gas and put it into an air bag for storage and subsequent indicator measurement, remove the marking equipment, and then collect plant, soil, and alkali solution (NaOH solution) samples.

[0026] Preferably, in step three, when the ambient temperature is high, the plastic film is covered with a double layer, the innermost layer is first fixed by the internal pressing frame, and then the outermost layer of plastic film is covered and the double-layer plastic film is fixed using the external pressing frame. At this time, a three-dimensional cavity is formed between the inner and outer plastic films, a trachea joint is fixed on the outermost layer of plastic film to connect to the air pump, and an exhaust port with the same diameter as the trachea joint is opened on the corresponding side, and the air pump is used to transport gas to make the gas in the cavity circulate, so as to achieve the effect of lowering the internal temperature of the isotope labeling box.

[0027] (3) Beneficial effects

[0028] Compared with the prior art, the present invention provides a 13 The convenient device and method for C isotope labeling have the following beneficial effects:

[0029] 1. The cube bracket designed and manufactured by the present invention adopts multiple horizontal tubes screwed on the threaded tubes on multiple vertical tubes to form a cube bracket, and an isotope generator and an air circulation device are installed at the diagonal point. Compared with traditional fixed equipment, it can be conveniently disassembled and carried, saving a lot of space. Multiple groups can be carried at one time to perform marking operations on different positions or plants. At the same time, by setting the cube bracket into an internally connected structure, the air circulation device can be used to make the marking gas flow back into the isotope labeling box from four directions and blow it toward the vicinity of the tested plant, which can quickly mix the gas in the isotope labeling box and improve the marking quality.

[0030] 2. The isotope generator of the present invention uses a syringe with a needle to directly puncture the rubber stopper. After passing through the rubber stopper, concentrated sulfuric acid can be pushed into the guide tube. The guide tube uses the upper cone to accommodate more concentrated sulfuric acid, and then slowly drips into the lower cone through the flow restriction hole. At this time, the concentrated sulfuric acid drips from multiple liquid outlets, which can slowly react with Na2 13 CO3 chemical reaction occurs when it comes into contact with the drug, so avoid adding too much concentrated sulfuric acid and Na2 13 The CO3 chemical reacts violently, and the marker gas generated during the reaction enters the gas cylinder through a right-angle elbow and comes into contact with the sodium hydroxide solution. In this way, the sodium hydroxide solution can be used to absorb the impurities in the gas, and then the purer marker gas is discharged from the outlet pipe into the isotope labeling box.

[0031] 3. During the design of this technical solution, a flexibly detachable cubic bracket is made using common durable pipes, and is assembled into an isotope labeling box with economical, practical and recyclable plastic films. The labeling box is made without a large amount of heavy main materials, so it is light and easy to move. The hard pipes and plastic films are used, and compared with traditional acrylic sheets and other materials, the products are strong and durable, and there is no problem of easy damage during movement. It is suitable for transportation and movement needs in scenarios such as field sample replacement. It is equipped with an edge-sealed pressure frame made of steel bar welding technology, which is strong and durable, can be reused many times, and can effectively ensure the sealing between the plastic film and the ground. The size of the labeling frame can be flexibly adjusted according to the applicable field scene, and is suitable for in-situ labeling experiments in various field ecosystems. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the convenient device for C isotope labeling;

[0033] Figure 2 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the cube support and edge-pressing assembly in the convenient device for C isotope labeling;

[0034] Figure 3 A method for outdoor use proposed by the present invention 13 Schematic diagram of the isotope generator in the convenient device for C isotope labeling Figure 1 ;

[0035] Figure 4 A method for outdoor use proposed by the present invention 13 Convenient device for C isotope labeling Figure 3 Schematic diagram of the structure at A in the middle;

[0036] Figure 5 A method for outdoor use proposed by the present invention 13 Schematic diagram of the isotope generator in the convenient device for C isotope labeling Figure 2 ;

[0037] Figure 6 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the air circulation device in the convenient device labeled with C isotope;

[0038] Figure 7 A method for outdoor use proposed by the present invention 13 Schematic diagram of the internal structure of the bellows in the convenient device labeled with C isotope;

[0039] Figure 8 A method for outdoor use proposed by the present invention 13Schematic diagram of the structure of the C-shaped clamp and vertical tube in the convenient device for C isotope labeling;

[0040] Figure 9 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the No. 2 blower and sealing cover in the convenient device labeled with C isotope;

[0041] Figure 10 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the vertical tube and horizontal tube of the convenient device for C isotope labeling;

[0042] Figure 11 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the C isotope-labeled convenient device after the side pressure assembly, vertical tube and horizontal tube are disassembled;

[0043] Figure 12 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the plastic film in the convenient device for C isotope labeling;

[0044] Figure 13 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of a double-layer plastic film used in a convenient device for C isotope labeling;

[0045] Figure 14 A method for outdoor use proposed by the present invention 13 Schematic diagram of the structure of the external edge-pressing box of the convenient device for C isotope labeling.

[0046] In the figure: 1. Cube bracket; 2. Fixing frame; 3. Sealing plate; 4. External pressure edge frame; 5. Internal pressure edge frame; 6. Tie mouth; 7. Wedge; 8. Plastic film; 9. Horizontal tube; 10. Vertical tube; 11. Exhaust hole; 12. No. 2 fan; 13. Positioning ring; 14. C-shaped retaining ring; 15. Bellows; 16. Magnetic sealing ring; 17. Rectangular sleeve; 18. Rectangular sleeve; 19. Rubber ring; 20. Bottle cap; 21. Exhaust pipe; 22. Gas cylinder ; 23. Card seat; 24. Right-angle elbow; 25. Rubber seat; 26. Medicine storage tube; 27. Guide tube; 28. Rubber plug; 29. ​​Upper cone; 30. Lower cone; 31. Liquid outlet; 32. Flow limiting hole; 33. Temperature and humidity sensor; 34. Fan No. 1; 35. Opening; 36. Air inlet; 37. Sealing cover; 38. Threaded pipe; 39. Nut; 40. Cavity; 41. Insert; 42. Fixing seat; 43. Hand-tightening bolt; 44. Pressure plate. DETAILED DESCRIPTION

[0047] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0048] Example 1: Refer to the attached Figure 1-14 , a kind of 13 The convenient device for C isotope labeling uses an assembled three-dimensional frame. Compared with traditional fixed equipment, it can be easily disassembled and carried, saving a lot of space. Multiple sets can be carried at a time to mark different locations or plants.

[0049] The main technical solution of this convenient device includes a cubic bracket 1, which is assembled by multiple horizontal tubes 9 and multiple vertical tubes 10. Here, 8 horizontal tubes 9 are required and 4 vertical tubes are required to form an octagonal 12-sided cube. The horizontal tubes 9 and the vertical tubes 10 can be made of common materials such as stainless steel tubes, engineering plastic tubes, alloy tubes, etc. The cubic bracket 1 is covered with a plastic film 8, which is made of a plastic film with good light transmittance, such as polyethylene (PE) film, polyvinyl chloride (PVC) and other economical materials. The thickness of the film is 0.15-0.3mm, preferably 0.2mm, and needs to reach 85%-90%. The haze is about 5%-10%, which meets the light transmittance requirements while also having good puncture resistance. The cubic bracket 1 and the plastic film 8 are assembled into an isotope labeling box. The interior of the cubic bracket 1 is connected and an air inlet 36 is opened on the wall of one of the vertical tubes 10. The upper end of the vertical tube 10 is provided with The hemispherical sealing cover can prevent air leakage and avoid puncturing the plastic film 8. The lower end of the vertical tube 10 is provided with a conical structure of the bundle 6. The bundle 6 is conical, which is convenient for inserting the assembled cube bracket 1 into the ground for fixing. Four threaded tubes 38 are fixed on the wall of the vertical tube 10. The four threaded tubes 38 are grouped in twos, and the center lines of the two threaded tubes 38 in each group are perpendicular to each other, that is, the angle is 90 degrees. One group of threaded tubes 38 is vertically fixed to the sealing cover above the vertical tube 10. Another set of threaded tubes 38 is vertically fixed to the tube wall of the vertical tube 10 above the bundle opening 6. Nuts 39 are fixedly connected to the inner edges of the two tube openings of the horizontal tube 9. The nuts 39 cooperate with the tube wall of the threaded tube 38. Multiple exhaust holes 11 are opened on the tube wall of the multiple vertical tubes 10. The multiple exhaust holes 11 are all oriented towards the vertical center line of the cubic support 1. The air inlet 36 opened on the tube wall of the vertical tube 10 is used for the air flow transported by the air circulation device to enter and be discharged from the multiple exhaust holes 11.

[0050] like Figure 10As shown, after the hollow threaded tube 38 is connected with the nut 39, the vertical tube 10 can be connected with the inside of the horizontal tube 9. When the air circulation device is working to generate gas, the gas in the isotope label box first enters the inside of the cube bracket 1 and is then discharged from the exhaust hole 11. Since the cube bracket 1 is provided with 4 vertical tubes 10, it can flow back to the isotope label box from four directions and blow toward the vicinity of the tested plant, which can quickly mix the gas in the isotope label box and improve the labeling effect.

[0051] like Figure 1 、 Figure 2 and Figure 14 As shown, the edge pressing assembly used is divided into two parts, inner and outer, including an inner edge pressing frame 5 and an outer edge pressing frame 4. The outer edge pressing frame 4 adopts a splicing design, and the inner edge pressing frame 5 is sleeved on the cube bracket 1. The inner edge pressing frame 5 of the edge pressing assembly is welded with steel bars, and the side length is two centimeters longer than the side length of the cube bracket 1. When the inner edge pressing frame 5 is sleeved on the cube bracket 1, the plastic film 8 can be well applied to the cube bracket 1 to form a regular isotope labeling box. The outer edge pressing frame 4 is spliced ​​by two identical C-shaped strips, and the lower end of the C-shaped strip is fixedly connected with a pressure plate 44 of the same structure. The pressure plate 44 has sufficient grounding area. When pressing the plastic film 8, the sealing line can be effectively improved to avoid the inside of the isotope labeling box. Marking gas leakage, one C-shaped strip pressure plate 44 is fixedly connected to two fixing seats 42, and the other C-shaped strip pressure plate 44 is fixedly connected to two matching inserts 41, and the fixing seats 42 are connected with hand screw bolts 43. The four corners of the external pressure edge frame 4 are provided with nail holes, and wedges 7 are inserted into the nail holes. The wedges 7 can firmly fix the external pressure edge frame 4 to the ground and press the plastic film 8. The plastic film 8 covers a double layer, and the innermost layer is first fixed by the internal pressure edge frame 5, and then the outermost layer of plastic film 8 is covered. The double-layer plastic film 8 is fixed using the external pressure edge frame 4. At this time, a three-dimensional cavity 40 is formed between the inner and outer plastic films 8. A trachea joint is fixed on the outermost layer of plastic film 8 to connect the air pump (such as Figure 13 The isotope labeling box is provided with an air outlet having the same diameter as the tracheal connector on the corresponding side. At this time, the air pump draws the external gas into the cavity 40 between the double-layer plastic film 8. The flowing cold air can effectively reduce the temperature inside the isotope labeling box, so that the internal temperature of the isotope labeling box can be maintained at a suitable labeling temperature.

[0052] When the present invention is in use, an internal cube bracket 1 is made, and multiple horizontal tubes 9 are screwed onto the threaded tubes 38 on multiple vertical tubes 10 to form a cube bracket 1, and an isotope generator and an air circulation device are installed diagonally. Compared with traditional fixed equipment, it can be easily disassembled and carried, saving a lot of space. Multiple groups can be carried at one time to perform marking operations on different locations or plants. At the same time, by setting the cube bracket 1 into an internally connected structure, the air circulation device can be used to make the marking gas flow back into the isotope labeling box from four directions and blow it toward the vicinity of the plant to be tested, which can quickly mix the gas in the isotope labeling box and improve the marking quality.

[0053] Example 2: Based on Example 1, the difference is that;

[0054] Refer to the attached Figure 3-Figure 5 In order to facilitate the staff to add drugs multiple times, the technical solution sets an isotope generator. The isotope generator is installed on the vertical side of the cube bracket 1 to facilitate the addition of chemicals and maintain the sealing of the isotope label box when injecting chemicals;

[0055] The isotope generating device used includes a fixed frame 2, one side of the fixed frame 2 is fixed to the wall of one of the vertical tubes 10, one side of the fixed frame 2 is fixedly connected to a rectangular sleeve 17, a rectangular sleeve 18 is sleeved in the rectangular sleeve 17, one side of the rectangular sleeve 18 is fixedly connected to a sealing plate 3, one side of the sealing plate 3 is fixedly connected to a magnetic sealing ring 16 that can be attracted to the fixed frame 2, a rubber ring 19 is embedded in the inner edge of the pipe opening at one end of the rectangular sleeve 17, the rubber ring 19 is a hollow structure and is sleeved with the side wall of the rectangular sleeve 18, at this time, the sealing plate 3 and the rectangular sleeve 18 form a movable drawer structure, the rubber ring 19 provides sealing when opened, and the rubber ring 19 and the magnetic sealing ring 16 jointly provide sealing when closed, so that the labeling gas in the isotope labeling box does not leak during multiple dosing;

[0056] In addition, since the plastic film 8 is a whole piece of plastic film with a side length of 2 meters, a rectangular groove is provided on one side of the fixed frame 2. When assembling the isotope generator, a rectangular hole can be cut on site along the rectangular groove using a cutter, and the rectangular hole can be pasted on the fixed frame 2 using transparent tape to ensure airtightness. A rubber seat 25 is fixedly connected to the rectangular sleeve 18, and a drug storage tube 26 is provided in the rubber seat 25. The drug storage tube 26 is used to store Na2 13CO3 medicine, a circular opening is provided at the upper end of the rectangular sleeve 18, a rubber plug 28 is provided in the circular opening, the lower end of the rubber plug 28 extends to the upper end of the medicine storage tube 26 and is fixedly connected to a guide tube 27, the guide tube 27 is composed of an upper cone 29 and a lower cone 30, a limited flow hole 32 is provided at the connection between the upper cone 29 and the lower cone 30, a cone bottom is provided at the lower end of the lower cone 30, a plurality of liquid outlet holes 31 are obliquely provided at the cone bottom, an air outlet is provided on one side of the medicine storage tube 26, a rubber ring is fixedly connected in the air outlet, a right-angle elbow 24 is sleeved in the rubber ring, a card seat 23 is provided on one side of the rubber seat 25, a gas cylinder 22 is carded in the card seat 23, and the gas cylinder 22 is used to store sodium hydroxide solution, and sodium hydroxide is used to produce a chemical reaction with sulfur dioxide to remove 13 The sulfur dioxide gas volatilized from the concentrated sulfuric acid in CO3, the upper end of the gas cylinder 22 is sealed with a bottle cap 20, one end of the right-angle elbow 24 passes through the bottle cap 20 and extends into the gas cylinder 22, and an outlet pipe 21 is installed on the other side of the bottle cap 20.

[0057] The isotope generator of the present invention uses a syringe with a needle to directly puncture the rubber stopper 28. After passing through the rubber stopper 28, the concentrated sulfuric acid can be pushed into the guide tube 27. The guide tube 27 uses the upper cone 29 to accommodate more concentrated sulfuric acid, and then slowly drips into the lower cone 30 through the flow restriction hole 32. At this time, the concentrated sulfuric acid drips from the multiple liquid outlet holes 31, thereby allowing the concentrated sulfuric acid to slowly react with Na2 13 CO3 chemical reaction occurs when it comes into contact with the drug, so avoid adding too much concentrated sulfuric acid and Na2 13 The CO3 drug produces a violent reaction. The marker gas generated during the reaction enters the gas cylinder 22 through the right-angle elbow 24 and comes into contact with the sodium hydroxide solution. In this way, the sodium hydroxide solution can be used to absorb the impurities in the gas. Then, the relatively pure marker gas is discharged from the outlet pipe 21 into the isotope labeling box.

[0058] Example 3: Based on Example 1, the difference is that;

[0059] Refer to the attached Figure 6-9 , air circulation device, the air circulation component is installed on the cube bracket 1 at a position diagonally opposite to the isotope generator. The internally connected cube bracket 1 allows the labeling gas to be quickly mixed in the isotope labeling box. The air circulation device includes a bellows 15, one side of the bellows 15 is fixedly connected to a C-shaped clamping ring 14, the C-shaped clamping ring 14 is clamped on the wall of the vertical tube 10 and has an opening 35 that matches the air inlet 36. A partition is fixedly connected to the bellows 15, which divides the bellows 15 into a drying chamber and an air inlet chamber. One side of the bellows 15 is fixedly connected to a fan No. 1 34, and the air outlet end of the fan No. 1 34 faces the air inlet 36 and the opening 35;

[0060] A medicine placement port is provided at the upper end of the bellows 15, a sealing cover 37 is installed at the medicine placement port, a No. 2 fan 12 is installed at the upper end of the sealing cover 37, a plurality of air inlets are provided on the side wall of the bellows 15, a mesh plate is fixedly connected to each of the plurality of air inlets, two temperature and humidity sensors 33 are installed at the air inlet, two positioning rings 13 are fixedly connected to the pipe wall of the vertical tube 10, and the positioning rings 13 are each provided with a corresponding positioning protrusion that cooperates with the opening 35 area of ​​the C-shaped clamping ring 14.

[0061] The present invention is provided with an air circulation device, which uses the No. 1 fan 34 to extract the gas in the isotope label box through the opening 35 and the air inlet into the interior of the cube bracket 1. As the No. 1 fan 34 continuously pushes the gas flow, the gas in the cube bracket 1 is discharged from the exhaust hole 11. Since the cube bracket 1 is provided with 4 vertical pipes 10, it can flow back to the isotope label box from four directions and blow toward the vicinity of the tested plant, which can make the gas in the isotope label box circulate and mix quickly, thereby improving the labeling quality. In addition, since the isotope label box is in a sealed state, its internal temperature will be higher than the external temperature. At this time, the high temperature will accelerate the evaporation of the soil moisture inside it, causing the humidity to increase. Therefore, a desiccant, such as a silica gel desiccant, can be placed in the drying chamber, and the gas in the drying chamber is extracted and discharged by the No. 2 fan 12. The humid air in the isotope label box passes through the mesh plate and enters the drying chamber. In this way, a drying cycle can be formed by the No. 2 fan 12 to avoid the influence of high humidity. 13 The stability of CO2 gas can be checked. At the same time, a temperature sensor 33 can be used to detect the temperature and humidity in the isotope labeling box. The start and stop circuit of the No. 2 fan 12 is controlled by the intelligent controller. This technology has been widely used in life and is already known to those skilled in the art. Therefore, it will not be described in detail. In addition, the No. 1 fan, the No. 2 fan and the temperature and humidity sensor are all powered by a mobile power supply.

[0062] The present invention also provides a method for outdoor use. 13 C isotope labeling method, this method is suitable for field research in grasslands, small trees, small shrubs, wetlands and other ecosystems. 13 The specific steps of the C isotope in situ labeling experiment are as follows:

[0063] Step 1: Make an internal cubic frame 1 by screwing multiple horizontal tubes 9 onto the threaded tubes 38 on multiple vertical tubes 10 to form a cubic frame 1, and install an isotope generator and an air circulation device at opposite corners;

[0064] Step 2: In the plot to be marked, select an area of ​​about one square meter, buckle the cube bracket 1 above the marked plant, insert the vertical tube 10 of the cube bracket 1 into the soil using the tie mouth 6 until the bottom edge is flush with the ground, and complete the fixing of the cube bracket 1. Clean a buffer zone of about 10 cm along the periphery of the bottom edge to facilitate the subsequent sealing with plastic film 8. Before that, clean a 10 cm piece of plastic film in the corner of the space covered by the cube bracket. 2 Place a 10ml 1mol L –1 A small bottle of sodium hydroxide (NaOH) solution is covered with an aluminum can with a diameter of 8 cm and a height of 10 cm and compacted to the ground to absorb soil respiration CO2;

[0065] Step 3: Cover the cube bracket 1 with a transparent plastic film 8, and use the internal pressing frame 5 to flatten and compact the transparent plastic film along the top surface and vertical edges of the cube bracket 1 from top to bottom. When the ambient temperature is high, the plastic film 8 covers a double layer, and the innermost layer is first fixed by the internal pressing frame 5, and then the outermost layer of plastic film 8 is covered and the double-layer plastic film 8 is fixed using the external pressing frame 4. At this time, a three-dimensional cavity 40 is formed between the inner and outer plastic films 8, and an air pipe joint is fixed on the outermost layer of plastic film 8 to connect to the air pump, and an exhaust port with the same diameter as the air pipe joint is opened on the corresponding side. The air pump is used to transport gas to circulate the gas in the cavity 40, so as to reduce the internal temperature of the isotope labeling box;

[0066] Step 4: Insert the external edge pressing frame 4, fix the four corners of the external edge pressing frame 4 to the ground with wedges 7, and then firmly fix the extended transparent plastic film 8 to the ground to complete the assembly of the isotope labeling box;

[0067] Step 5: Use a syringe needle to quantitatively inject 5 ml of concentrated sulfuric acid into the drug storage tube 26 in the isotope generator. The concentrated sulfuric acid and the Na2 13 CO3 reacts to produce 13 The marking process was completed by CO2 gas, and this step was repeated 3 times 1, 2, and 4 hours after the first marking.

[0068] Step 6: 6 hours after the first marking, pierce the transparent plastic film with a syringe, take 100 ml of gas and put it into an air bag for storage and subsequent indicator measurement, remove the marking equipment, and then collect plant, soil, and alkaline NaOH solution samples.

[0069] Compared with the existing technology, the design process of this technical solution uses common durable pipes to make a flexibly detachable cubic bracket 1, and uses an economical, practical and recyclable plastic film 8 to assemble it into an isotope labeling box. The production of this labeling box does not require a large amount of heavy main materials, so it is light and easy to move.

[0070] The use of hard pipes and plastic films makes the products stronger and more durable than traditional acrylic sheets. They are not prone to damage during movement and are suitable for transportation and movement in scenarios such as field site changes.

[0071] It is equipped with an edge-sealing frame made of steel bar welding technology, which is strong and durable, can be reused many times, and can effectively ensure the sealing between the plastic film and the ground;

[0072] The size of the labeling frame can be flexibly adjusted according to the applicable field scene, and is suitable for in situ labeling experiments in a variety of field ecosystems. Since isotope labeling needs to be carried out as simultaneously as possible and in a unified manner, it is recommended to prepare sufficient frames and sufficient materials and drugs in advance, and then carry out the unified operation. 13 C isotope labeling.

[0073] It should be noted that the term "comprises" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article, or apparatus that includes a series of elements includes not only those elements, but also includes other elements not explicitly listed, or also includes elements inherent to such process, method, article, or apparatus. In the absence of further limitations, an element defined by the phrase "comprises a ..." does not exclude the presence of other identical elements in the process, method, article, or apparatus that includes the element.

[0074] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A type used in the wild 13 A convenient device for C isotope labeling, characterized in that include: A cubic bracket (1), wherein the cubic bracket (1) is assembled by a plurality of horizontal tubes (9) and a plurality of vertical tubes (10), the cubic bracket (1) is covered with a plastic film (8), the cubic bracket (1) and the plastic film (8) are assembled into an isotope labeling box, the interior of the cubic bracket (1) is connected, and an air inlet (36) is opened on the wall of one of the vertical tubes (10); a plurality of exhaust holes (11) are opened on the wall of the plurality of vertical tubes (10), and the plurality of exhaust holes (11) are all oriented toward the vertical center line of the cubic bracket (1), and the air inlet (36) opened on the wall of the vertical tube (10) is used for the air flow transported by the air circulation device to enter and be discharged from the plurality of exhaust holes (11); A side pressing assembly, the side pressing assembly comprising an inner side pressing frame (5) and an outer side pressing frame (4), the outer side pressing frame (4) adopts a splicing design, and the inner side pressing frame (5) is sleeved on the cubic bracket (1); An isotope generating device, comprising a fixed frame (2), one side of the fixed frame (2) being fixed on the wall of one of the vertical tubes (10), one side of the fixed frame (2) being fixedly connected to a rectangular sleeve (17), a rectangular sleeve (18) being sleeved in the rectangular sleeve (17), one side of the rectangular sleeve (18) being fixedly connected to a sealing plate (3), a rubber seat (25) being fixedly connected in the rectangular sleeve (18), a medicine storage tube (26) being arranged in the rubber seat (25), a circular opening being opened at the upper end of the rectangular sleeve (18), a rubber plug (28) being arranged in the circular opening, and the rubber plug (2 8) extends to the upper end of the drug storage tube (26) and is fixedly connected to a guide tube (27), the guide tube (27) is composed of an upper cone (29) and a lower cone (30), a limited flow hole (32) is provided at the connection between the upper cone (29) and the lower cone (30), the lower end of the lower cone (30) is provided with a cone bottom, and a plurality of liquid outlet holes (31) are obliquely opened at the cone bottom, and an air outlet is provided on one side of the drug storage tube (26), the isotope generator is installed on the vertical side of the cubic bracket (1) to facilitate the addition of chemicals, and can maintain the sealing of the interior of the isotope label box when injecting chemicals; An air circulation device, comprising a bellows (15), one side of the bellows (15) being fixedly connected to a C-shaped snap ring (14), the C-shaped snap ring (14) being snap-fitted to the wall of the vertical tube (10) and having an opening (35) matching the air inlet (36), a partition being fixedly connected inside the bellows (15), the partition dividing the bellows (15) into a drying chamber and an air inlet chamber, a fan (34) being fixedly connected to one side of the bellows (15), an air outlet end of the fan (34) facing the air inlet (36) and the opening (35); The upper end of the bellows (15) is provided with a medicine placement port, a sealing cover (37) is installed at the medicine placement port, and a second fan (12) is installed at the upper end of the sealing cover (37). The side wall of the bellows (15) is provided with multiple air inlets, and mesh plates are fixedly connected to the multiple air inlets. Two temperature and humidity sensors (33) are installed at the air inlets. Two positioning rings (13) are fixedly connected to the tube wall of the vertical tube (10), and the positioning rings (13) are correspondingly provided with positioning protrusions that match the opening (35) area of ​​the C-shaped clamping ring (14). The air circulation device is installed on the cube bracket (1) at a position diagonal to the isotope generating device, and the labeling gas can be quickly mixed in the isotope labeling box through the internally connected cube bracket (1).

2. A method for use in the field according to claim 1 13 A convenient device for C isotope labeling, characterized by: The upper end of the vertical tube (10) is provided with a hemispherical sealing cover, and the lower end of the vertical tube (10) is provided with a conical structure of the bundle opening (6). Four threaded tubes (38) are fixed on the tube wall of the vertical tube (10). The four threaded tubes (38) are grouped in pairs, and the center lines of the two threaded tubes (38) in each group are perpendicular to each other. One group of the threaded tubes (38) is vertically fixed to the sealing cover above the vertical tube (10), and the other group of the threaded tubes (38) is vertically fixed to the tube wall of the vertical tube (10) above the bundle opening (6). The inner edges of the two tube openings of the horizontal tube (9) are fixedly connected with nuts (39), and the nuts (39) are matched with the tube walls of the threaded tubes (38).

3. A method for use in the field according to claim 2 13 A convenient device for C isotope labeling, characterized by: The inner edge pressing frame (5) of the edge pressing assembly is welded with steel bars, and the side length is two centimeters longer than the side length of the cube bracket (1). The outer edge pressing frame (4) is spliced ​​with two identical C-shaped bars, and the lower end of the C-shaped bar is fixedly connected with a pressure plate (44) of the same structure, wherein two fixing seats (42) are fixedly connected to the pressure plate (44) of one of the C-shaped bars, and two matching inserts (41) are fixedly connected to the pressure plate (44) of the other C-shaped bar, and the fixing seat (42) is connected with a hand-tightening bolt (43). The four corners of the outer edge pressing frame (4) are provided with nail holes, and wedges (7) are inserted into the nail holes.

4. A method for use in the field according to claim 3 13 A convenient device for C isotope labeling, characterized by: One side of the sealing plate (3) is fixedly connected to a magnetic sealing ring (16) that can be attracted to the fixed frame (2). A rubber ring (19) is embedded in the inner edge of the pipe opening at one end of the rectangular sleeve (17). The rubber ring (19) is a hollow structure and is sleeved with the side wall of the rectangular sleeve (18). A rectangular groove is provided on one side of the fixed frame (2).

5. A convenient device for outdoor use according to any one of claims 4 13 C isotope labeling method, this method is suitable for field research in grassland, small trees, small shrubs, and wetland ecosystems. 13 C isotope in situ labeling experiment, characterized in that The specific steps are as follows; Step 1: Make an internal cubic support (1), screw multiple horizontal tubes (9) onto the threaded tubes (38) on multiple vertical tubes (10) to form a cubic support (1), and install an isotope generator and an air circulation device at opposite corners; Step 2: In the plot to be marked, select an area of ​​about one square meter, buckle the cube bracket (1) above the marked plant, insert the vertical tube (10) of the cube bracket (1) into the soil using the tie mouth (6) until the bottom edge is flush with the ground, and complete the fixing of the cube bracket (1). Clear a buffer zone of about 10 cm along the periphery of the bottom edge to facilitate subsequent sealing with plastic film (8); Step 3: Cover the cube support (1) with a transparent plastic film (8), and use the internal pressing frame (5) to smooth and compact the transparent plastic film along the top surface and vertical edges of the cube support (1) from top to bottom; Step 4: Insert the external pressing frame (4), fix the four corners of the external pressing frame (4) to the ground with wedges (7), and then firmly fix the extended transparent plastic film (8) to the ground to complete the assembly of the isotope labeling box; Step 5: Use a syringe needle to quantitatively inject 5 ml of concentrated sulfuric acid into the drug storage tube (26) in the isotope generator. The concentrated sulfuric acid and the Na2 13 CO3 reacts to produce 13 The marking process was completed by CO2 gas, and this step was repeated 3 times 1, 2, and 4 hours after the first marking. Step 6: 6 hours after the first marking, pierce the transparent plastic film with a syringe, take 100 ml of gas and put it into an air bag for storage and subsequent indicator measurement, remove the marking equipment, and then collect plant, soil, and alkali solution (NaOH solution) samples.

6. The method for field 13C isotope labeling according to claim 5, characterized in that: In the step 3, when the ambient temperature is high, the plastic film (8) is covered with a double layer, the innermost layer is first fixed by the internal pressing frame (5), and then the outermost layer of plastic film (8) is covered and the double-layer plastic film (8) is fixed by the external pressing frame (4). At this time, a three-dimensional cavity (40) is formed between the inner and outer plastic films (8), and a trachea joint is fixed on the outermost layer of plastic film (8) to connect to the air pump, and an exhaust port with the same diameter as the trachea joint is opened on the corresponding side. The air pump is used to transport gas to circulate the gas in the cavity (40), so as to reduce the internal temperature of the isotope labeling box.

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