Performance testing device for ultra-low temperature cell freezing membrane

Abstract

The present invention relates to the field of cell cryopreservation membrane detection technology, specifically to a performance detection device for ultra-low temperature cell cryopreservation membranes, comprising a fixed seat, the fixed seat being located at the bottom end of the entire device, a top block being installed on the fixed seat, a lifting assembly being installed between the top block and the fixed seat, and a detection platform being fixedly installed on the top end of the top block. The detection device provided in this device cuts off the power supply of the simulated freezer during the heating process of the light source heating head, and the environment is heated. After a few minutes, it moves to the next station to observe the cell cryopreservation membrane through a microscope lens, and the cell membrane slide is placed on the detection disk, and the detection disk is rotated by the rotating assembly at the bottom, which is convenient for rotary detection of the cell membrane slide, thereby improving the efficiency of the detection. The rotating assembly is provided on the detection platform, and the detection platform can be height-adjusted by the lifting assembly at the bottom, which is convenient for the user to adjust the height according to his or her own height, so as to facilitate the convenience of detection.

Description

Technical Field

[0001] The present invention relates to the technical field of cell cryopreservation membrane detection, in particular to a performance detection device for ultra-low temperature cell cryopreservation membrane. Background Art

[0002] Ultra-low temperature cell freezing membrane is generally used to store and preserve cell fluid, and has the effect of preserving the cell fluid. Cell cryopreservation is a technology that uses a cryoprotection mechanism to place cells in a low temperature environment, reduce cell metabolism, maintain cell activity, and allow for long-term storage.

[0003] However, the current ultra-low temperature cell freezing membranes need to be tested and observed after storage. However, most of the current detection devices use microscopes to detect one by one. During the detection process, only the front of the ultra-low temperature cell freezing membrane can be detected. The angle of the ultra-low temperature cell freezing membrane cannot be adjusted, and the dynamic situation of the internal cell fluid cannot be easily observed.

[0004] Maintaining an ultra-low ambient temperature while preserving the cell fluid is crucial. When cryogenic equipment malfunctions, the ambient temperature rises rapidly, requiring the cryofilm to provide a certain degree of insulation. This allows the temperature rise within the cryofilm (bags and boxes) to be minimized during equipment maintenance to ensure cell viability. Conventional detection devices can only simply monitor cell status and lack the ability to simulate localized ambient temperature rises. Therefore, improvements are needed to provide more comprehensive detection capabilities. Summary of the Invention

[0005] The purpose of the present invention is to provide a performance detection device for ultra-low temperature cell freezing membrane to solve the problems raised in the above background technology.

[0006] To achieve the above-mentioned object, the present invention provides the following technical solution: a performance testing device for low-temperature cell cryopreservation membranes, comprising a fixing base, the fixing base being located at the bottom end of the entire device, a top block being mounted on the fixing base, a lifting assembly being mounted between the top block and the fixing base, a testing platform being fixedly mounted on the top end of the top block, a rotating assembly being mounted within the testing platform, a connecting column being mounted on the rotating assembly, and a testing disc being fixedly mounted on the top end of the connecting column, the testing disc being located in a slot in the middle of the testing platform, and a plurality of cell membrane slides containing cell fluids loaded from cryopreservation membranes being mounted on the testing disc;

[0007] The top of the cell membrane slide is detected by a light source heating head and a microscope lens, the light source heating head and the microscope lens are on a fixed frame, and the fixed frame is fixedly installed on the detection table, the detection plate is provided with a plurality of storage slots, the storage slots are provided with side end slots, and the side end slots are installed with a lifting assembly;

[0008] The lifting assembly is respectively equipped with a first movable frame and a second movable frame, the first movable frame and the second movable frame are rotatably connected via a rotating shaft, a cylinder is fixedly mounted on the fixed seat, a gas rod is mounted on the output end of the cylinder, and the protruding end of the gas rod is sleeved through the sleeve on the first movable frame and rotatably connected through the rotating shaft;

[0009] A first motor is installed on the rotating assembly, a main shaft is installed on the output end of the first motor, and a first bevel gear is fixedly installed on the main shaft, a second bevel gear is meshed with the bottom end of the first bevel gear, and the second bevel gear is located on the chassis;

[0010] The lifting assembly is arranged across the side end groove, and the lifting assembly includes a second motor. A threaded rod is installed at the output end of the second motor, and two threaded blocks are respectively threadedly connected to the threaded rod, and the two threaded blocks are respectively connected to the first lifting block and the second lifting block. The first lifting block and the second lifting block are respectively in contact with the left and right end supports of the bottom of the cell membrane slide.

[0011] Furthermore, the chassis of the present invention is provided with a slide rail, and the slide rail is slidably connected to the movable member and the sliding block respectively, and the first motor is fixedly mounted on the sliding block.

[0012] Furthermore, in the present invention, a connecting frame is provided between the main shaft at the output end of the first motor and the movable part, and a connecting column connected to the detection disk is fixedly installed on the top of the connecting frame.

[0013] Furthermore, the inner ends of the fixing seat and the top block of the present invention are both provided with side sliding grooves, and the outer ends of the first movable frame and the second movable frame are fixedly provided with protruding blocks which are slidably connected to the side sliding grooves.

[0014] Furthermore, a panel is fixedly installed on the front of the detection platform of the present invention, and the first motor and the cylinder are both controlled by the panel.

[0015] Furthermore, the threaded rod of the present invention is located on a bottom block, a sliding groove is provided on the bottom block, and the sliding groove is slidably connected to the bottom end protruding block at the bottom end of the threaded block.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] (1) The present invention is a performance detection device for ultra-low temperature cell cryopreservation membranes. The detection device provided in the device cuts off the power supply to the simulated freezer during the heating process of the light source heating head, and the environment is heated. After a few minutes, it moves to the next station to observe the cell cryopreservation membrane through the microscope lens. The cell membrane slide is placed on the detection disk, and the detection disk is rotated by the rotating component at the bottom, which facilitates the rotation detection of the cell membrane slide and improves the efficiency of the detection.

[0018] (2) The present invention is a performance testing device for ultra-low temperature cell cryopreservation membranes. The rotating assembly provided in the device is located on a testing platform. The testing platform can be height-adjusted by a lifting assembly at the bottom, so that users can adjust the height according to their own height to facilitate the convenience of testing.

[0019] (3) The present invention is a performance testing device for ultra-low temperature cell freezing membranes. The lifting component provided in the device can be a small structure that can be lifted up at one angle or multiple angles, which is convenient for observing the flow state of the internal cells, and for detecting whether the dynamic performance of the internal cells will be immediately affected after the external light temperature rises, and in turn, the performance of the freezing membrane bag itself is tested. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 2 is a schematic structural diagram of a device for detecting the performance of an ultra-low temperature cell freezing membrane according to an embodiment of the present invention;

[0022] Figure 2 2 is a schematic structural diagram of a rotating assembly of a device for detecting the performance of an ultra-low temperature cell freezing membrane according to an embodiment of the present invention;

[0023] Figure 3 According to the present invention Figure 2 A schematic diagram of the structure enlarged in the middle;

[0024] Figure 4 Schematic diagram of the structure of the detection disk in the performance detection device of the ultra-low temperature cell freezing membrane of the present invention;

[0025] Figure 5 It is a structural schematic diagram of the jacking assembly in the present invention.

[0026] Reference numerals:

[0027] 1. Fixed seat; 2. First movable frame; 3. Second movable frame; 4. Rotating shaft; 5. Cylinder; 6. Air rod; 7. Raised block; 8. Side slide; 9. Top block; 10. Detection table; 11. Panel; 12. Detection plate; 13. Cell membrane slide; 14. Fixed frame; 15. Light source heating head; 16. Chassis; 17. Slide rail; 18. Movable part; 19. Sliding block; 20. First motor; 21. Spindle; 22. First bevel gear; 23. Second bevel gear; 24. Connecting frame; 25. Connecting column; 26. Storage slot; 27. Microscope lens; 28. Side end slot; 29. ​​Bottom block; 30. Bottom end raised block; 31. Second motor; 32. Threaded rod; 33. Threaded block; 34. First lifting block; 35. Second lifting block; 36. Slide. DETAILED DESCRIPTION

[0028] The embodiments of the present invention are described in detail below. The embodiments are implemented based on the technical solutions of the present invention, and detailed implementation methods and specific operating processes are given. However, the protection scope of the present invention is not limited to the following embodiments.

[0029] like Figures 1 to 5 The performance testing device for cryogenic cell cryopreservation membrane shown in the figure includes a fixing base 1, which is located at the bottom end of the entire device. A top block 9 is installed on the fixing base 1, and a lifting assembly is installed between the top block 9 and the fixing base 1. A detection platform 10 is fixedly installed on the top of the top block 9. A rotating assembly is installed in the detection platform 10, and a connecting column 25 is installed on the rotating assembly. A detection tray 12 is fixedly installed on the top of the connecting column 25. The detection tray 12 is located in the middle slot of the detection platform 10. A plurality of cell membrane slides 13 containing cell fluid loaded by cryopreservation membrane are installed on the detection tray 12;

[0030] The top of the cell membrane slide 13 is detected by a light source heating head 15 and a microscope lens 27. The light source heating head 15 and the microscope lens 27 are on a fixed frame 14, and the fixed frame 14 is fixedly mounted on the detection table 10. The detection plate 12 is provided with a plurality of storage slots 26, and the storage slots 26 are provided with side end slots 28, and the side end slots 28 are installed with a lifting assembly;

[0031] The lifting assembly is respectively equipped with a first movable frame 2 and a second movable frame 3, which are rotatably connected via a rotating shaft 4. A cylinder 5 is fixedly mounted on the fixed seat 1, and a gas rod 6 is mounted on the output end of the cylinder 5. The protruding end of the top of the gas rod 6 is sleeved through the sleeve on the first movable frame 2 and penetrates the rotating shaft 4 for rotatable connection.

[0032] A first motor 20 is mounted on the rotating assembly. A main shaft 21 is mounted on the output end of the first motor 20. A first bevel gear 22 is fixedly mounted on the main shaft 21. A second bevel gear 23 is engaged with the bottom end of the first bevel gear 22. The second bevel gear 23 is located on the chassis 16.

[0033] The lifting assembly is arranged across the side end groove 28, and the lifting assembly includes a second motor 31. A threaded rod 32 is installed at the output end of the second motor 31, and two threaded blocks 33 are respectively threadedly connected to the threaded rod 32, and the two threaded blocks 33 are respectively connected to the first lifting block 34 and the second lifting block 35. The first lifting block 34 and the second lifting block 35 are respectively in contact with the left and right end supports of the bottom of the cell membrane slide 13.

[0034] A slide rail 17 is provided on the chassis 16 , and the slide rail 17 is slidably connected to a movable member 18 and a sliding block 19 , respectively. A first motor 20 is fixedly mounted on the sliding block 19 .

[0035] A connecting frame 24 is provided between the main shaft 21 at the output end of the first motor 20 and the movable member 18 , and a connecting column 25 connected to the detection disk 12 is fixedly mounted on the top of the connecting frame 24 .

[0036] The inner ends of the fixing seat 1 and the top block 9 are both provided with side sliding grooves 8 , and the outer ends of the first movable frame 2 and the second movable frame 3 are fixedly mounted with protruding blocks 7 and are slidably connected to the side sliding grooves 8 .

[0037] A panel 11 is fixedly mounted on the front of the testing platform 10 , and the first motor 20 and the cylinder 5 are both controlled by the panel 11 .

[0038] The threaded rod 32 is located on the bottom block 29 , and a sliding groove 36 is provided on the bottom block 29 . The sliding groove 36 is slidably connected to the bottom end protruding block 30 at the bottom end of the threaded block 33 .

[0039] Working principle:

[0040] 1. The detection device provided in this apparatus observes the cell cryopreservation membrane through the light source heating head 15 and the microscope lens 27. The light source heating head 15 uses strong light to locally heat the cell membrane slide containing the cell fluid on the lower station, simulating the failure of the refrigeration equipment, until the preset heating time is reached.

[0041] 2. The detection disk 12 is rotated by the rotating assembly at the bottom, and the rotating assembly is driven by the first motor 20 to drive the first bevel gear 22 on the main shaft 21 to rotate. Since the bottom end of the first bevel gear 22 is engaged with the second bevel gear 23, the first motor 20 is slidably connected to the slide rail 17 on the chassis 16 through the sliding block 19. When the first motor 20 drives the main shaft 21 to rotate, it will drive itself to rotate, thereby realizing the rotation of the detection disk 12 on the connecting column 25 at the top of the connecting frame 24, which is convenient for rotary detection of the cell membrane slide 13;

[0042] At this time, the cell membrane slide 13 that has reached the preset heating time is rotated to the next station, and is observed through the microscope lens 27 above the station to check the changes in the cells in the cell membrane slide after the simulated refrigeration equipment failure and local heating, and to detect and analyze whether the performance of the cryopreservation membrane loaded with cell fluid meets the standards.

[0043] 3. In order to observe from multiple angles and observe the flow state of the cell fluid, the lifting component is set across the side end groove 28, and the second motor 31 drives the threaded rod 32 to rotate. Since the bottom ends of the two threaded blocks 33 threadedly connected on the threaded rod 32 are slidably connected to the slide groove 36 through the bottom end protrusion block 30, the first lifting block 34 and the second lifting block 35 on the two threaded blocks 33 are simultaneously moved in the same direction. When the first lifting block 34 lifts the cell membrane slide 13 on which the cell cryopreservation membrane is placed, the cell membrane slide 13 can be tilted in one direction, and vice versa, the other end of the cell membrane slide 13 can be tilted. The small structure that can be lifted at one or multiple angles is convenient for observing the flow state of the internal cells. To detect whether the dynamic performance of the internal cells will be immediately affected after the external light temperature rises, so as to detect the performance of the cryopreservation membrane bag itself;

[0044] The first lifting block 34 and the second lifting block 35 can also adjust the preset spacing according to the size of the actual cell membrane slide 13, with good matching, and can be applied to the detection of cell membrane slides 13 of various sizes;

[0045] The rotating component is arranged on the testing platform 10, and the testing platform 10 can realize height adjustment through the lifting component at the bottom. The air cylinder 5 drives the air rod 6 to drive the adjustment of the first movable frame 2 on the air rod 6. Since the first movable frame 2 and the second movable frame 3 are rotatably connected by the rotating shaft 4, and the two side ends of the first movable frame 2 and the second movable frame 3 are slidably connected to the side slide grooves 8 on both sides of the fixed seat 1 and the top block 9 through the protruding blocks 7, the height adjustment of the testing platform 10 is realized, which is convenient for the user to adjust the height according to his own height, so as to facilitate the convenience of detection.

Claims

1. A device for detecting the performance of ultra-low temperature cell freezing membranes, characterized in that: The device comprises a fixed seat (1), wherein the fixed seat (1) is located at the bottom end of the entire device, a top block (9) is installed on the fixed seat (1), a lifting assembly is installed between the top block (9) and the fixed seat (1), a detection table (10) is fixedly installed on the top end of the top block (9), a rotating assembly is installed in the detection table (10), a connecting column (25) is installed on the rotating assembly, and a detection disk (12) is fixedly installed on the top end of the connecting column (25), the detection disk (12) is located in the middle slot of the detection table (10), and a plurality of cell membrane slides (13) loaded with cell fluid by cryopreservation membrane are installed on the detection disk (12); The top of the cell membrane slide (13) is detected by a light source heating head (15) and a microscope head (27), the light source heating head (15) and the microscope head (27) are on a fixing frame (14), and the fixing frame (14) is fixedly mounted on the detection table (10), a plurality of storage slots (26) are provided on the detection plate (12), side end slots (28) are provided in the storage slots (26), and a lifting assembly is installed in the side end slots (28); A first movable frame (2) and a second movable frame (3) are respectively installed on the lifting assembly, and the first movable frame (2) and the second movable frame (3) are rotatably connected via a rotating shaft (4). A cylinder (5) is fixedly installed on the fixed seat (1), and a gas rod (6) is installed at the output end of the cylinder (5), and the top protruding end of the gas rod (6) is sleeved through the kit on the first movable frame (2) and penetrates through the rotating shaft (4) for rotatable connection; A first motor (20) is mounted on the rotating assembly, a main shaft (21) is mounted on the output end of the first motor (20), a first bevel gear (22) is fixedly mounted on the main shaft (21), a second bevel gear (23) is meshed with the bottom end of the first bevel gear (22), and the second bevel gear (23) is located on the chassis (16); The lifting assembly is arranged across the side end groove (28), and the lifting assembly includes a second motor (31). The output end of the second motor (31) is installed with a threaded rod (32), and the threaded rod (32) is respectively threadedly connected to two threaded blocks (33), and the two threaded blocks (33) are respectively connected to a first lifting block (34) and a second lifting block (35), and the first lifting block (34) and the second lifting block (35) are respectively in contact with the left and right end supports of the bottom of the cell membrane slide (13); Furthermore, the end surfaces of the first lifting block (34) and the second lifting block (35) at the points where they contact the left and right end supports of the bottom of the corresponding cell membrane carrier (13) are relatively arranged arc surface structures.

2. The performance detection device of the ultra-low temperature cell freezing membrane according to claim 1, characterized in that: A slide rail (17) is provided on the chassis (16), and the slide rail (17) is slidably connected to a movable member (18) and a sliding block (19), respectively, and a first motor (20) is fixedly mounted on the sliding block (19).

3. The performance detection device of the ultra-low temperature cell freezing membrane according to claim 1, characterized in that: A connecting frame (24) is provided between the main shaft (21) at the output end of the first motor (20) and the movable member (18), and a connecting column (25) connected to the detection disk (12) is fixedly mounted on the top of the connecting frame (24).

4. The performance detection device of the ultra-low temperature cell freezing membrane according to claim 1, characterized in that: The inner ends of the fixed seat (1) and the top block (9) are both provided with side sliding grooves (8), and the outer ends of the first movable frame (2) and the second movable frame (3) are fixedly mounted with protruding blocks (7) and are slidably connected to the side sliding grooves (8).

5. The performance detection device of the ultra-low temperature cell freezing membrane according to claim 1, characterized in that: A panel (11) is fixedly mounted on the front of the detection platform (10), and the first motor (20) and the cylinder (5) are both controlled by the panel (11).

6. The performance detection device of the ultra-low temperature cell freezing membrane according to claim 1, characterized in that: The threaded rod (32) is located on the bottom block (29). A sliding groove (36) is provided on the bottom block (29). The sliding groove (36) is slidably connected to the bottom end protruding block (30) at the bottom end of the threaded block (33).

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