Selenized codonopsis pilosula polysaccharide and Sijunzi decoction composition as well as preparation method and application of selenized codonopsis pilosula polysaccharide and Sijunzi decoction composition

Abstract

The invention discloses a selenized codonopsis pilosula polysaccharide and Sijunzi decoction composition as well as a preparation method and application thereof. The composition is prepared from codonopsis pilosula, bighead atractylodes rhizome, poria cocos, liquorice and selenized codonopsis pilosula polysaccharide. Each 1000 mL of the liquid medicine contains 15-20 g of codonopsis pilosula, 10-15 g of bighead atractylodes rhizome, 10-15 g of poria cocos, 5-10 g of liquorice and 4-6 mg of selenized codonopsis pilosula polysaccharide. The polysaccharide content in the liquid medicine is not lower than 0.5%, and the selenium content is not lower than 0.05%. The preparation method comprises the following steps: decocting with water to extract codonopsis pilosula, bighead atractylodes rhizome, poria cocos and liquorice, and mixing with selenized codonopsis pilosula polysaccharide. The selenized codonopsis pilosula polysaccharide and sijunzi decoction composition disclosed by the invention is derived from natural traditional Chinese medicines, and has no toxic or side effect and no hidden danger of medicine residues; the prescription is refined, the quality is controllable, the dosage form is advanced, and administration is convenient; when the newcastle disease vaccine is singly used or matched with the newcastle disease vaccine to immunize chicks, the weight can be obviously increased, the serum antibody titer can be improved, the thymus organ index can be increased, the white marrow proportion of spleen tissues can be increased, the cellular immunity and humoral immunity can be obviously enhanced, and the immune response of the vaccine can be improved.

Description

Technical Field

[0001] The present invention belongs to the field of livestock and poultry immune adjuvants, and particularly relates to a composition of selenium-enriched codonopsis pilosula polysaccharide and sijunzi decoction, a preparation method thereof, and an application thereof. Background Art

[0002] There are no effective therapeutic drugs for livestock and poultry immunosuppressive diseases, especially those caused by viral infections. In addition to strengthening feeding management to improve the disease resistance of animals, vaccination is currently the most economical and effective intervention measure for preventing and controlling infectious diseases. With the continuous update and development of modern biotechnology, the types of domestic animal vaccines are increasing day by day, such as new vaccines like subunit vaccines, polypeptide vaccines, and protein vaccines, which have higher safety compared to traditional vaccines such as inactivated vaccines and attenuated or live-attenuated vaccines. However, their immunogenicity is poor. In most cases, they need to be assisted by immune adjuvants to effectively activate the cellular immunity and humoral immunity responses of the body to the target antigen and improve the immune effect. Considering the advantages of traditional Chinese medicine such as safety, multiple active ingredients, and multiple targets, it can be considered as a new type of immune enhancer.

[0003] Selenium is an essential trace element in the body, which has functions such as antioxidant, anti-aging, and immune regulation. It participates in the formation of the active center of peroxidase in the body. Appropriate use can enhance the body's resistance and antioxidant capacity. Selenium polysaccharide, as a kind of organic selenium, is a complex of easily obtained but highly toxic inorganic selenium and polysaccharide, maintaining the basic structure of the polysaccharide while optimizing the effective bioavailability of trace element selenium and improving the pharmacological activity of the polysaccharide, which is more conducive to the body's digestion and absorption and plays the dual roles of plant polysaccharide and trace element selenium. After the polysaccharide is selenized and modified, a selenium-enriched polysaccharide containing both selenium and polysaccharide components can be obtained, which is easily absorbed and utilized by the body. Research shows that appropriate modification of the polysaccharide molecule and reconstruction of the polysaccharide molecule structure will produce new polysaccharide activities or further enhance some existing activities. Codonopsis pilosula belongs to the family Campanulaceae and the genus Codonopsis. The traditional medicinal part is its root. The medicinal functions of codonopsis pilosula have been recorded in ancient and modern medical books. "Ben Cao Cong Xin" describes that codonopsis pilosula has the functions of replenishing the middle qi and relieving vexation and thirst of the spleen and stomach. "Ben Cao Gang Mu Shi Yi" describes that the functions of codonopsis pilosula can replace ginseng, with yellow skin color and transverse stripes similar to those of ledebouriella seseloides, so it is named fangdang. Modern pharmacological research confirms that codonopsis pilosula polysaccharide is the main active ingredient of codonopsis pilosula, with biological activities such as antioxidant, anti-tumor, enhancing the body's immunity, prolonging the lifespan of the body, protecting the liver, and improving reproductive function. Summary of the Invention

[0004] Object of the Invention: The first object of the present invention is to provide a composition of selenium-enriched codonopsis pilosula polysaccharide and sijunzi decoction with high-efficiency and safe immune enhancement effects; the second object of the present invention is to provide a preparation method of the composition of selenium-enriched codonopsis pilosula polysaccharide and sijunzi decoction; the third object of the present invention is to provide an application of the composition of selenium-enriched codonopsis pilosula polysaccharide and sijunzi decoction.

[0005] Technical solution: The composition of polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction is made of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Glycyrrhiza uralensis and polysaccharide of Codonopsis pilosula with selenium; every 1000 mL of the medicinal liquid contains 15 - 20 g of Codonopsis pilosula, 10 - 15 g of Atractylodes macrocephala, 10 - 15 g of Poria cocos, 5 - 10 g of Glycyrrhiza uralensis and 4 - 6 mg of polysaccharide of Codonopsis pilosula with selenium; the polysaccharide content in the medicinal liquid is not less than 0.5%, and the selenium content is not less than 0.05%.

[0006] Preferably, every 1000 mL of the medicinal liquid contains 20 g of Codonopsis pilosula, 12 g of Atractylodes macrocephala, 12 g of Poria cocos, 6 g of Glycyrrhiza uralensis and 5 mg of polysaccharide of Codonopsis pilosula with selenium.

[0007] The preparation method of the composition of polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction includes water decocting Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis, and mixing with the polysaccharide of Codonopsis pilosula with selenium to obtain the composition of polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction.

[0008] Preferably, Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis are water decocted, filtered, concentrated, then the polysaccharide of Codonopsis pilosula with selenium is added, mixed evenly, sub-packed and sterilized to obtain the composition of polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction.

[0009] Preferably, the specific method for water decocting Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis is: adding 15 - 25 times the mass of water, soaking, boiling for extraction, extracting twice in total, boiling for 45 - 60 min for the first time and boiling for 30 - 45 min for the second time.

[0010] Preferably, the specific method for water decocting Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis is: adding 20 times the mass of water, soaking for 25 - 35 min, boiling for extraction, extracting twice in total, boiling for 45 min for the first time and boiling for 30 min for the second time.

[0011] Preferably, the polysaccharide of Codonopsis pilosula with selenium is obtained by selenium-modifying the polysaccharide of Codonopsis pilosula. The specific modification method is: dissolving the polysaccharide of Codonopsis pilosula in HNO3 solution, adding Na2SeO3 for reaction, adjusting the pH to 5 - 6 after the reaction is completed, centrifuging to take the supernatant, and dialyzing until there is no residual selenite in the dialysate to obtain the polysaccharide of Codonopsis pilosula with selenium.

[0012] Preferably, the mass ratio of the polysaccharide of Codonopsis pilosula to Na2SeO3 is 5:2 - 4.

[0013] Preferably, the mass ratio of the polysaccharide of Codonopsis pilosula to Na2SeO3 is 5:3.

[0014] Preferably, the concentration of the polysaccharide of Codonopsis pilosula in the HNO3 solution is 10 mg / mL.

[0015] Preferably, the HNO3 solution is 0.5% HNO3 solution.

[0016] Preferably, the reaction temperature is 70 - 80 °C, the reaction lasts for 6 - 8 hours, and after the reaction solution is cooled, the pH is adjusted to 5 - 6.

[0017] Use of the polysaccharide of Codonopsis pilosula selenized and the composition of Sijunzi Decoction in enhancing the immune response of livestock and poultry vaccines.

[0018] The present invention conducts selenization modification on the polysaccharide of Codonopsis pilosula, establishes a method for selenization modification of the polysaccharide of Codonopsis pilosula, and discovers that selenization modification can significantly enhance the immune activity of the polysaccharide of Codonopsis pilosula. The selenium polysaccharide has relatively strong immune activity enhancement. Through comprehensive comparison in aspects such as screening from component drugs to formula screening and dosage form screening, combined with cost and convenient application, etc., the present invention successfully develops an immune enhancer composed of the polysaccharide of Codonopsis pilosula selenized and the composition of Sijunzi Decoction, proving that the immune activity enhancement of the compound is stronger than the individual action of each.

[0019] Beneficial effects: Compared with the prior art, the present invention has the following remarkable advantages:

[0020] (1) Compared with chemical adjuvants, the immune enhancer composed of the polysaccharide of Codonopsis pilosula selenized and the composition of Sijunzi Decoction has reliable effects, and is derived from natural traditional Chinese medicine, without toxic and side effects and the hidden danger of drug residues, ensuring the food safety of animal sources; the production has no pollution to the environment and meets the requirements of green environmental protection.

[0021] (2) Compared with traditional Chinese medicine formulas, the immune enhancer composed of the polysaccharide of Codonopsis pilosula selenized and the composition of Sijunzi Decoction has a refined prescription, controllable quality, advanced dosage form, and convenient administration.

[0022] (3) Compared with formulas of similar traditional Chinese medicine components, the immune enhancer composed of the polysaccharide of Codonopsis pilosula selenized and the composition of Sijunzi Decoction has improved efficacy. When used alone or in combination with Newcastle disease vaccine to immunize chicks, it can significantly increase body weight, improve the serum antibody titer, increase the thymus organ index, increase the proportion of white pulp in the spleen tissue, significantly enhance cellular immunity and humoral immunity, and improve the immune response of the vaccine. Description of the Drawings

[0023] Figure 1 It is the histological changes of the spleen tissues of each group in the second week (HE staining, magnification: 100X);

[0024] Figure 2 It is the histological changes of the spleen tissues of each group in the fourth week (HE staining, magnification: 100X). Detailed Embodiments

[0025] The following further illustrates the technical solutions of the present invention with reference to the drawings and embodiments.

[0026] Extraction of the polysaccharide of Codonopsis pilosula:

[0027] Use the water decoction - ethanol precipitation method. Take 1000 g of Codonopsis pilosula, crush it into 1 mm 3Weigh the Codonopsis pilosula roots of appropriate size and place them in a forced-air drying oven for drying at 60 °C. After drying, soak the Codonopsis pilosula roots in 95% ethanol overnight, and carry out degreasing by refluxing in a water bath at 70 °C for 3 times, 1 hour each time, until the reflux liquid is nearly colorless. Collect the medicinal residues and air-dry them. Add 20 times the amount of water to the pretreated Codonopsis pilosula medicinal residues, reflux in a water bath at 80 °C for 3 times, 1 hour each time, filter by suction, discard the medicinal residues, combine the filtrates and concentrate them to 1 g / mL. Slowly add absolute ethanol until the final ethanol concentration is 80%, place it at 4 °C and let it stand overnight, filter by suction, wash the precipitate with acetone 2 times each, and freeze-dry to obtain crude Codonopsis pilosula polysaccharide CPPSc. Remove proteins under the conditions of 3.5% cellulase, pH 6, temperature 45 °C, and time 0.5 h to obtain Codonopsis pilosula polysaccharide (CCPS).

[0028] Selenization modification of Codonopsis pilosula polysaccharide:

[0029] Weigh 500 mg of CPPSpl, transfer it to a three-necked flask, slowly add 50 mL of 0.5% HNO3 solution while stirring, dissolve the solution completely, add 300 mg of Na2SeO3, react at 70 °C for 8 h. After the reaction is completed, cool it to room temperature, adjust the pH value to 5 - 6 with anhydrous sodium bicarbonate solution, centrifuge, load the supernatant solution into a dialysis bag, and use the ascorbic acid method to detect whether selenite ions are contained in the dialysis solution. When the detection solution does not show red, that is, when there is no residue of selenite ions, stop dialysis.

[0030] Example 1

[0031] The composition of selenized Codonopsis pilosula polysaccharide and Sijunzi Decoction is made from Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Glycyrrhiza uralensis and selenized Codonopsis pilosula polysaccharide; every 1000 mL of the medicinal liquid contains 20 g of Codonopsis pilosula, 12 g of Atractylodes macrocephala, 12 g of Poria cocos, 6 g of Glycyrrhiza uralensis and 5 mg of selenized Codonopsis pilosula polysaccharide; the extraction process of the extracts of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis is to soak with 15 - 25 times the mass of water for 30 min, boil for extraction, and extract 2 times in total. Boil for 45 min for the first time and 30 min for the second time. Mix the two extraction solutions, filter, and concentrate to 1000 mL. Dissolve 5 mg of selenized Codonopsis pilosula polysaccharide in 1000 mL of the Sijunzi Decoction extract, subpackage, and sterilize to obtain it.

[0032] Comparison of immune enhancement effects:

[0033] In vivo immune enhancement test:

[0034] Method: Codonopsis pilosula polysaccharide selenide and the immune enhancer of Sijunzi Decoction composition were used as test materials, both with a 1000 mL stock solution. The period from 1 to 14 days old was the brooding stage. The indoor temperature and humidity were moderate, suitable for the growth and development of chicks. During this period, the chicks were active, freely fed and drank water, and there was no abnormal death. After 14 days, the chicken house temperature was maintained at 25 °C and the humidity was normal. The chicks were randomly divided into 4 groups: blank group, immune group, high-dose Sijunzi Decoction group (10%), low-dose Sijunzi Decoction group (5%), high-dose composition group (10%), and low-dose composition group (5%). Each group had 36 chicks, with an equal number of males and females. The mental state of the chicken flock was good, and the feeding and drinking conditions were normal.

[0035] On the 7th, 14th, 21st, 28th, 35th, 42nd, and 56th days after administration, 6 broiler chickens were randomly selected from each group and blood was collected from the wing vein. After the collected blood was allowed to stand at room temperature for 30 min, it was centrifuged at 3000 r / min for 10 min to separate and collect the serum, which was then dispensed into EP tubes. The antibody titer was measured, and the chickens were weighed to compare the differences between groups. On the 7th, 14th, 21st, 28th, 35th, 42nd, and 56th days, the heart, liver, spleen, thymus, bursa of Fabricius and other tissue organs were dissected, the surrounding adipose tissue was stripped, and they were weighed to calculate the corresponding organ indices. The spleen was fixed with formalin, stained with HE, and histological analysis was performed.

[0036] Results:

[0037] K was the blank control group, M was the immune control group, YH was the high-dose Sijunzi Decoction group, YL was the low-dose Sijunzi Decoction group, YH+SE was the high-dose composition group, and YL+SE was the low-dose composition group. The immune enhancement effects of different doses of codonopsis pilosula polysaccharide selenide and the immune enhancer of Sijunzi Decoction composition on the immune effect of Newcastle disease vaccine in chickens were compared. It was proved that appropriate doses could significantly increase body weight, improve serum antibody titer, increase thymus organ index, increase the proportion of white pulp in spleen tissue, enhance cellular and humoral immunity in chickens, and improve the immune response of Newcastle disease vaccine; 5% was the optimal dose for chicks (Tables 1 - 4). Specifically as follows:

[0038] (1) Measuring antibody titer

[0039] The changes in serum antibodies of each group are shown in Table 1. Except in the first week, the antibody titers of the immune control group were significantly higher than those of the blank control group at other time points (p < 0.05). At the 1st, 3rd, 5th, and 6th weeks, the serum antibody titers of each administration group were significantly higher than those of the immune control group (p < 0.05). At the 2nd and 4th weeks, except for the low-dose composition group, there was no significant difference in serum antibody titers between other administration groups and the immune control group (p > 0.05). At the 2nd week, the serum antibody titer of the low-dose composition group was significantly higher than those of the high-dose composition group and the low-dose Sijunzi Decoction group (p < 0.05). At the 3rd, 4th, 5th, and 6th weeks, the composition group was higher than the Sijunzi Decoction group, but the difference was not significant (p > 0.05).

[0040] Table 1 Antibody Titers in Each Group

[0041]

[0042] (2) Effects on Immune Organ Indices

[0043] The organ indices in the second week are shown in Table 2. From the perspective of the heart index, the high-dose Codonopsis pilosula polysaccharide group was significantly lower than the blank control group (p<0.05), and there was no significant difference from other groups. For the liver index, it can be seen that the low-dose selenium-enriched Codonopsis pilosula polysaccharide group was significantly higher than the blank dose group (p<0.05); the lung organ indices in the immune control group were significantly lower than those in the blank control group (p<0.05); there were no significant differences in the spleen index, thymus index, and bursa of Fabricius index between each drug-treated group and the blank control group and the immune control group.

[0044] Table 2 Organ Indices in the Second Week

[0045]

[0046] The organ indices in the fourth week are shown in Table 3. From the perspective of the thymus index, the high-dose Codonopsis pilosula polysaccharide group and the high- and low-dose selenium-enriched Codonopsis pilosula polysaccharide groups were significantly higher than the low-dose Codonopsis pilosula polysaccharide group, the immune control group, and the blank control group (p<0.05). There were no significant differences in the heart, liver, spleen, lung, and bursa of Fabricius organ indices between each drug-treated group and the blank dose group and the immune dose group.

[0047] Table 3 Organ Indices in the Fourth Week

[0048]

[0049] (3) Histological Changes in the Spleen

[0050] The spleen is mainly responsible for governing blood and is an important peripheral lymph organ of the body. It is mainly composed of a capsule, trabeculae, white pulp, and red pulp. The white pulp includes: the lymphatic sheath mainly contains T cells, the lymphoid nodules mainly contain B cells, and the marginal zone mainly contains lymphocytes and macrophages. An increase in white pulp indicates enhanced immune activity of the body. The red pulp includes: the splenic cords contain B cells, plasma cells, macrophages, and dendritic cells, and the splenic sinusoids are surrounded by long rod-shaped endothelial cells and drain into the trabecular veins. The histological changes in the spleen of each group at 2 and 4 weeks after immunization are as Figure 1-2 shown ( Figures 1 to 2 Upper left is K: blank control group; Figures 1 to 2 Lower left is M: immune control group; Figures 1 to 2 Middle upper is YH: high-dose Sijunzi Decoction group; Figures 1 to 2 Middle lower is YH+SE: high-dose composition group; Figures 1 to 2 Upper right is YL: low-dose Sijunzi Decoction group; Figures 1 to 2Lower right is YL+SE (low-dose composition group). At weeks 2 and 4, it was found that the periarteriolar lymphatic sheath in the white pulp of the immunized group thickened, and T lymphocytes divided and proliferated. The structure of the red pulp became wider, interconnected into a network, and the number of lymphoid tissue cells and macrophages increased. The medicated group was higher than the immunized group and the blank control group, and the composition group was significantly higher than the Sijunzi Decoction group. The low-dose composition group was the best.

[0051] The above results indicate that the immunopotentiator of polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction composition can significantly improve the immune function of neonatal chickens immunized with Newcastle disease vaccine at appropriate doses. The effect is the best at 5%, which is the optimal dose.

[0052] Example 2

[0053] The polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction composition is made from Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Glycyrrhiza uralensis and polysaccharide of Codonopsis pilosula with selenium; each 1000 mL of the medicinal liquid contains 15 g of Codonopsis pilosula, 10 g of Atractylodes macrocephala, 10 g of Poria cocos, 5 g of Glycyrrhiza uralensis and 4 mg of polysaccharide of Codonopsis pilosula with selenium; the extraction process of the extracts of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis is to soak with 15 times the mass of water for 25 min and extract by boiling, with a total of 2 extractions. The first boiling is for 45 min and the second boiling is for 30 min. The two extraction solutions are mixed evenly, filtered, and concentrated to 1000 mL. 4 mg of polysaccharide of Codonopsis pilosula with selenium is added to 1000 mL of the Sijunzi Decoction extract for dissolution, sub-packed, and sterilized to obtain the polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction composition.

[0054] Example 3

[0055] The polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction composition is made from Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, Glycyrrhiza uralensis and polysaccharide of Codonopsis pilosula with selenium; each 1000 mL of the medicinal liquid contains 17 g of Codonopsis pilosula, 15 g of Atractylodes macrocephala, 15 g of Poria cocos, 10 g of Glycyrrhiza uralensis and 6 mg of polysaccharide of Codonopsis pilosula with selenium; the extraction process of the extracts of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Glycyrrhiza uralensis is to soak with 25 times the mass of water for 35 min and extract by boiling, with a total of 2 extractions. The first boiling is for 60 min and the second boiling is for 45 min. The two extraction solutions are mixed evenly, filtered, and concentrated to 1000 mL. 6 mg of polysaccharide of Codonopsis pilosula with selenium is added to 1000 mL of the Sijunzi Decoction extract for dissolution, sub-packed, and sterilized to obtain the polysaccharide of Codonopsis pilosula with selenium and Sijunzi Decoction composition.

Claims

1. A selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition, characterized in that: It is made of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos, liquorice and selenized Codonopsis pilosula polysaccharide; each 1000mL of the medicinal solution contains 15-20g of Codonopsis pilosula, 10-15g of Atractylodes macrocephala, 10-15g of Poria cocos, 5-10g of liquorice and 4-6mg of selenized Codonopsis pilosula polysaccharide; the polysaccharide content in the medicinal solution is not less than 0.5%, and the selenium content is not less than 0.05%.

2. The composition of selenized Codonopsis pilosula polysaccharide and Sijunzi decoction according to claim 1, characterized in that: Each 1000mL of the medicinal solution contains 20g of Codonopsis pilosula, 12g of Atractylodes macrocephala, 12g of Poria cocos, 6g of Licorice root and 5mg of selenized Codonopsis pilosula polysaccharide.

3. A method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 1, characterized in that: The method comprises the steps of decocting and extracting codonopsis pilosula, atractylodes macrocephala, poria cocos and liquorice, and mixing the extracts with selenized codonopsis pilosula polysaccharide to obtain a composite material of selenized codonopsis pilosula polysaccharide and Sijunzi decoction.

4. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 3, characterized in that: Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Licorice root are extracted by decoction, filtered and concentrated, and then selenized Codonopsis pilosula polysaccharide is added, mixed, packaged and sterilized to obtain a composition of selenized Codonopsis pilosula polysaccharide and Sijunzi decoction.

5. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 3, characterized in that: The specific method for decoction extraction of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Licorice is: adding 15-25 times the mass of water, soaking, boiling and extracting, extracting twice in total, the first boiling time is 45-60 minutes, and the second boiling time is 30-45 minutes.

6. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 3, characterized in that: The specific method for decoction extraction of Codonopsis pilosula, Atractylodes macrocephala, Poria cocos and Licorice is: add 20 times the mass of water, soak for 25-35 minutes, boil and extract, extract twice in total, boil for 45 minutes for the first time and boil for 30 minutes for the second time.

7. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 3, characterized in that: The selenized Codonopsis pilosula polysaccharide is obtained by selenization modification of Codonopsis pilosula polysaccharide, and the specific modification method is: dissolving Codonopsis pilosula polysaccharide in HNO3 solution, adding Na2SeO3 for reaction, adjusting the pH to 5-6 after the reaction is completed, centrifuging to obtain the supernatant, dialyzing until no selenite remains in the dialyzate, and obtaining the selenized Codonopsis pilosula polysaccharide.

8. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 7, characterized in that: The mass ratio of the codonopsis pilosula polysaccharide to Na2SeO3 is 5:2-4.

9. The method for preparing the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 7, characterized in that: The reaction temperature is 70-80° C., the reaction time is 6-8 hours, and the pH value of the reaction solution is adjusted to 5-6 after the reaction solution is cooled.

10. Use of the selenized Codonopsis pilosula polysaccharide and Sijunzi decoction composition according to claim 1 in improving the immune response of livestock and poultry vaccines.

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