Preparation method and device of multicoloanyl phyllin antioxidant

Abstract

The invention relates to the technical field of plant extracts, and discloses a preparation method and device of a multicoloured leaf-based phytol antioxidant, and the preparation method comprises the following steps: pretreatment: selecting fresh or dry multicoloured leaves as a raw material, and carrying out cleaning, drying and crushing treatment to obtain multicoloured leaf powder with the modulus of 80; cellulose enzymolysis: mixing the multicoloured leaf powder with water according to a ratio of 1: 2 to 1: 20, and adding a proper amount of one or more of vitamin enzyme, cellulose disacidase, xylanase, dextransucrase, fructolase, glutathione peroxidase, papain and fig enzyme for enzymolysis treatment. The mode of combining enzymolysis treatment and alcohol solution extraction is adopted, the destructive effect of enzymolysis on plant cell walls is fully utilized, and the dissolution rate and extraction efficiency of phytol are greatly improved. The phytol can be extracted from the multicoloured leaves more quickly and efficiently, and the extraction time and the solvent consumption are reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of plant extracts, and particularly to a preparation method and device for antioxidant of pandan leaf base phytol. Background Art

[0002] Pandan leaf, also known as pandanus leaf, with the scientific name of Pandanus amaryllifolius Roxb, is a natural plant resource rich in various bioactive components such as phytol, squalene, and vitamin K3. Hainan is the origin and dominant production area of pandan leaf cultivation in China. The planting areas mainly include Wanning, Qionghai, Danzhou, Lingshui, Baoting, Ding'an and other places. Among them, Wanning, as the main distribution area and traditional utilization area, has a planting area of about 5,000 mu.

[0003] With the increasing demand for healthy and natural products, natural antioxidants have been increasingly widely used in the fields of food, cosmetics, and medicine due to their advantages such as safety, non-toxic side effects, and good biocompatibility. Phytol, as an antioxidant compound naturally present in plants, has attracted much attention due to its excellent antioxidant performance. At present, the methods for extracting phytol from pandan leaf and preparing antioxidants are not yet perfect, and there are problems such as low extraction efficiency and difficult purification. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides a preparation method and device for antioxidant of pandan leaf base phytol, which solves the problems that the methods for extracting phytol from pandan leaf and preparing antioxidants are not yet perfect, and there are problems such as low extraction efficiency and difficult purification.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: A preparation method for antioxidant of pandan leaf base phytol, comprising the following steps:

[0006] Pretreatment: Select fresh or dried pandan leaf as raw material, and carry out cleaning, drying and crushing treatments to obtain pandan leaf powder with a modulus of 80.

[0007] Enzymatic hydrolysis of cellulose: Mix the pandan leaf powder with water in a ratio of 1:2 to 1:20, and add an appropriate amount of one or more of cellulase, cellobiase, xylanase, dextran sucrase, fructase, glutathione peroxidase, papain, ficin for enzymatic hydrolysis treatment.

[0008] Extraction with alcohol solution: Immerse the enzymatic hydrolysis residue in an ethanol solution with a concentration of 30% to 95%, and carry out heating reflux extraction at a temperature of 65°C to 73°C for 5 hours to obtain pandan extract.

[0009] Concentration and removal of chlorophyll: The pandan extract is subjected to vacuum concentration to remove most of the solvent and precipitated chlorophyll, obtaining a concentrated solution.

[0010] Purification: The concentrated solution is purified using chromatographic separation technology to obtain phytol with a relatively high purity.

[0011] Preferably, the method includes the determination of antioxidant capacity. The purified phytol solution is mixed with the FRAP working solution in a certain proportion and reacted at an appropriate temperature. The antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength of 593 nm before and after the reaction, the antioxidant capacity of the purified phytol can be calculated.

[0012] Furthermore, the preparation of the FRAP working solution generally includes acetate buffer solution (pH 3.6), TPTZ (2,4,6-tripyridyltriazine) solution and iron ion solution. These solutions are mixed under specific conditions to form a blue-violet Fe(II)-TPTZ complex.

[0013] Preferably, in the enzymatic hydrolysis step, the enzyme addition amount is 0.1% to 2% of the substrate, the enzymatic hydrolysis temperature is controlled between 35°C and 75°C, and the enzymatic hydrolysis time is 2 hours to 8 hours. In the alcohol solution extraction step, the ethanol solution concentration is 30% to 95%, the heating temperature is controlled between 65°C and 73°C, and the extraction time is 5 hours.

[0014] Furthermore, controlling the enzyme addition amount in the enzymatic hydrolysis step within the range of 0.1% to 2% can ensure that the enzyme fully degrades the cell wall in the pandan leaves and releases more active ingredients. Controlling the enzymatic hydrolysis temperature between 35°C and 75°C enables the full exertion of the enzyme's activity while avoiding the inactivation of the enzyme at too high a temperature. Setting the enzymatic hydrolysis time to 2 hours to 8 hours can ensure sufficient time to fully release active ingredients such as phytol while avoiding the loss of other ingredients or unnecessary side reactions due to too long a time. In the alcohol solution extraction step, controlling the ethanol solution concentration within the range of 30% to 95% can ensure the full dissolution of phytol while avoiding the influence of high-concentration solvents on other plant active ingredients. Controlling the heating temperature between 65°C and 73°C effectively improves the solubility of phytol while protecting the stability of heat-sensitive ingredients. Setting the extraction time to 5 hours ensures the efficient release of active ingredients such as phytol in the pandan leaves, optimizes the use of solvents, and reduces the energy consumption during the extraction process.

[0015] Preferably, the concentration step uses vacuum concentration technology to reduce the loss of heat-sensitive ingredients and further remove chlorophyll by filtration, obtaining a concentrated solution rich in phytol.

[0016] Furthermore, the concentration step uses vacuum concentration technology, which effectively avoids the degradation of thermosensitive components caused by high temperature, reduces the solvent usage, and lowers the energy consumption. The vacuum concentration technology can complete the concentration process at a relatively low temperature, ensuring the stability of components such as phytol during the concentration process. After concentration, the precipitated chlorophyll and impurities are removed through further filtration. The resulting concentrated solution is rich in phytol and provides a purer raw material for the subsequent purification step, improving the effect of subsequent purification.

[0017] Preferably, the purification step adopts chromatographic separation technology to remove impurities and improve the purity of phytol.

[0018] Furthermore, the purification step adopts chromatographic separation technology, which can effectively remove impurities in the extract and improve the purity of phytol. The chromatographic separation technology precisely separates phytol from other impurities by selecting appropriate solvents and separation media, ensuring a high purity of phytol in the extract. This process can ensure that the finally obtained phytol has high biological activity and stability, meets the purity requirements in various industrial applications, and enhances the market competitiveness of the product.

[0019] A device for extracting phytol from pandan leaves, comprising an extraction tank, the lower surface of the extraction tank is fixed with support feet, and a heating plate is fixed on the outer wall of the extraction tank; a crushing assembly, which is arranged on the extraction tank and is used to crush pandan leaves into fine particles. The crushing assembly includes a hopper, the bottom end of the hopper penetrates through the top end of the extraction tank, a connecting plate is fixed at the top end of the extraction tank, a motor one is fixed inside the connecting plate, the output end of the motor one is fixed with a rotating rod one, a gear one is fixed at the end of the rotating rod one, a rotating rod two is rotatably arranged inside the hopper, a gear two is fixed at the end of the rotating rod two, the gear two meshes with the gear one, and crushing rollers are fixed on the outer walls of the rotating rod one and the rotating rod two; a stirring assembly, which is arranged inside the extraction tank and is used to enhance the contact between the extraction solvent and the pandan leaves.

[0020] Preferably, the stirring assembly includes a motor two, the outer wall of the motor two is fixed at the top end of the extraction tank, the driving end of the motor two is fixed with a driving gear, two symmetrically arranged stirring rods are rotatably arranged inside the extraction tank, a driven gear is fixed at the end of each stirring rod, the driven gears mesh with the driving gear, and a plurality of stirring blades are fixed on the outer walls of the stirring rods.

[0021] Preferably, a fixing plate is fixed on the outer wall of the support feet, a delivery pump is fixed on the upper surface of the fixing plate, an extraction pipe is fixed at the input end of the delivery pump, the end of the extraction pipe penetrates through the bottom end of the extraction tank, a delivery pipe is fixed at the output end of the delivery pump, and the output end of the delivery pipe is fixed with a filtration tank.

[0022] Preferably, a filter screen is fixed inside the filtration tank, a filter membrane is fixed inside the filtration tank, a discharge pipe penetrates through the bottom end of the filtration tank, and a valve is arranged inside the discharge pipe.

[0023] Preferably, a temperature sensor penetrates through one side of the extraction tank, and a pressure sensor penetrates through one side of the extraction tank, which is used to monitor the temperature and pressure changes in the extraction tank during the extraction process in real time to ensure stable extraction conditions. A controller is fixed on the outer wall of the extraction tank, and the controller is electrically connected to the pressure sensor and the temperature sensor.

[0024] The present invention provides a preparation method and device for the antioxidant of chlorophyllol from pandanus leaf bases. It has the following beneficial effects:

[0025] 1. The present invention combines enzymatic hydrolysis treatment with alcohol extraction, makes full use of the destructive effect of enzymatic hydrolysis on plant cell walls, and greatly improves the dissolution rate and extraction efficiency of chlorophyllol. It can extract chlorophyllol from pandanus leaves more quickly and efficiently, reduces the extraction time and solvent consumption, is conducive to promoting the development of pandanus leaf resources, and solves the problems of low extraction efficiency and difficult purification in traditional extraction methods.

[0026] 2. The present invention purifies chlorophyllol by using chromatographic separation technology, avoiding the cumbersome multi-step filtration and multiple solvent exchanges in traditional methods. This method not only improves the purification effect, simplifies the operation process, reduces manual intervention, but also ensures the acquisition of high-purity chlorophyllol.

[0027] 3. Through the cooperation between the stirring component and the crushing component, the present invention achieves the effect of crushing and cutting pandanus leaves, improves the efficiency of subsequent extraction, and solves the problems that the cell walls of pandanus leaves are not easily damaged and the extraction solvent does not contact the raw materials sufficiently in traditional extraction methods. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is the process flow chart of the present invention;

[0029] Figure 2 is the three-dimensional view of the present invention;

[0030] Figure 3 is the structural schematic diagram of the extraction tank of the present invention;

[0031] Figure 4 is the schematic diagram of the crushing component of the present invention;

[0032] Figure 5 is the internal structural schematic diagram of the extraction tank of the present invention;

[0033] Figure 6 is the structural schematic diagram of the filtration tank of the present invention.

[0034] Among them, 1. extraction tank; 2. support feet; 3. crushing assembly; 301. hopper; 302. connecting plate; 303. motor 1; 304. rotating rod 1; 305. gear 1; 306. rotating rod 2; 307. gear 2; 308. crushing roller; 4. stirring assembly; 401. motor 2; 402. driving gear; 403. stirring rod; 404. driven gear; 405. stirring blade; 5. fixing plate; 6. delivery pump; 7. extraction pipe; 8. delivery pipe; 9. filtration tank; 10. filter screen; 11. filter membrane; 12. discharge pipe; 13. valve; 14. heating plate; 15. temperature sensor; 16. pressure sensor; 17. controller. Detailed implementation mode

[0035] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the attached drawings of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work shall fall within the protection scope of the present invention.

[0036] Please refer to the attached Figure 1 , and the embodiments of the present invention provide a preparation method of antioxidant of pandanus leaf phytol.

[0037] Embodiment 1:

[0038] (1) Pretreatment: Select 100 g of fresh or dried pandanus leaves as raw materials, wash, dry and crush them to obtain pandanus leaf powder with a modulus of 80, soak the powder in 500 g of distilled water, and take it out after standing for 24 hours.

[0039] (2) Enzymatic hydrolysis of cellulose: Dissolve the soaked pandanus powder as a solute in a lytic enzyme solution containing 1% cellulase, glutathione peroxidase, papain, and ficin, control the temperature at 37 °C, and the reaction time is 2 hours.

[0040] (3) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water, stir and extract at 100 °C for 3 times, 2 hours each time, filter with a 200-mesh filter cloth to obtain a filtrate, adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0041] (4) Alcohol solution extraction: Mix the enzymatic hydrolysis residue, grape seed extract and 500 g of 30% ethanol solvent in a volume ratio of 1:1:1, stir evenly and stand for 2 hours. Filter the filter residue, collect the filtrate and perform vacuum concentration treatment to obtain a preliminary extract.

[0042] (V) Concentration to Remove Chlorophyll: The preliminary extract is concentrated under vacuum to remove most of the solvent and the precipitated chlorophyll. It is concentrated to 250 g and impurities such as chlorophyll are removed by filtration, finally obtaining 195 g of pandan concentrate rich in phytol.

[0043] (VI) Purification of Phytol: The concentrate is further purified by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine and other fields.

[0044] (VII) Determination of Antioxidant Capacity: The purified phytol solution is mixed with the FRAP working solution in a certain proportion and reacted at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced by per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0045] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0046] Control Example: Use 500 g of water to replace the alcohol solution for extraction, and keep other conditions unchanged. It will be found that the calculated FRAP value is significantly smaller than the value calculated by extracting with the alcohol solution.

[0047] Example 2:

[0048] (I) Pretreatment: Select 300 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. Immerse the powder in 900 g of distilled water and take it out after standing for 24 hours.

[0049] (II) Enzymatic Hydrolysis of Cellulose: Immerse the soaked pandan leaves in a solution containing 1% cellulase, glutathione peroxidase, papain, and ficin, control the temperature at 37 °C, and the reaction time is 2 hours.

[0050] (III) Grape Seed Extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water and stir and extract at 100 °C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate with 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain grape seed extract;

[0051] (4) Extraction with alcohol solution: Mix the enzymolysis residue, grape seed extract and 900 g of 30% ethanol solvent in a ratio of 1:1, stir evenly and let stand for 2 hours. Filter the residue, collect the filtrate and perform vacuum concentration to obtain a preliminary extract.

[0052] (5) Concentrate to remove chlorophyll: Remove most of the solvent and precipitated chlorophyll from the preliminary extract by vacuum concentration, concentrate to 350 g and filter to remove impurities such as chlorophyll, and finally obtain 295 g of pandan concentrate rich in phytol.

[0053] (6) Purify phytol: Further purify the concentrate by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine and other fields.

[0054] (7) Determination of antioxidant capacity: Mix the purified phytol solution and FRAP working solution in a certain ratio and react at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0055] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity

[0056] Control: Purify the concentrate by traditional extraction and separation method instead of chromatographic separation method, and keep other conditions unchanged. It will be found that the calculated FRAP value is significantly smaller than the value calculated by alcohol solution extraction.

[0057] Example 3:

[0058] (1) Pretreatment: Select 500 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. Immerse the powder in 1000 g of distilled water and take it out after standing for 24 hours.

[0059] (2) Enzymolysis of cellulose: Immerse the soaked pandan leaves in a solution containing 1% cellulase, glutathione peroxidase, papain, and ficin, control the temperature at 37 °C, and the reaction time is 2 hours.

[0060] (III) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water, stir and extract at 100 °C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0061] (IV) Ethanol solution extraction: Mix the enzymolysis residue, grape seed extract with 1000 g of 50% ethanol solvent in a ratio of 1:1, stir evenly and let stand for 2 hours. Filter the residue, collect the filtrate and perform vacuum concentration to obtain a preliminary extract.

[0062] (V) Concentrate to remove chlorophyll: Remove most of the solvent and precipitated chlorophyll from the preliminary extract by vacuum concentration, concentrate to 350 g and filter to remove impurities such as chlorophyll, and finally obtain 200 g of pandan concentrate rich in phytol.

[0063] (VI) Purify phytol: Further purify the concentrate by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine and other fields.

[0064] (VII) Determination of antioxidant capacity: Mix the purified phytol solution with the FRAP working solution in a certain ratio and react at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced by per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0065] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0066] Control example: Use 1000 g of water instead of ethanol solution for extraction, keep other conditions unchanged, and it will be found that the calculated FRAP value is significantly smaller than the value calculated by ethanol solution extraction.

[0067] Example 4:

[0068] (I) Pretreatment: Select 100 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. Soak the powder in 500 g of distilled water, and take it out after standing for 24 hours.

[0069] (II) Enzymatic hydrolysis of cellulose: Soak the soaked pandan leaves in a solution containing 1% xylanase, glutathione peroxidase, papain, and ficin, control the temperature at 37 °C, and the reaction time is 2 hours.

[0070] (III) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water and stir and extract at 100 °C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain grape seed extract;

[0071] (IV) Alcohol solution extraction: Mix the enzymatic hydrolysis residue, grape seed extract with 500 g of 50% ethanol solvent in a ratio of 1:1, stir evenly and then stand for 2 hours. Filter the residue, collect the filtrate and perform vacuum concentration to obtain a preliminary extract.

[0072] (V) Concentrate to remove chlorophyll: Remove most of the solvent and precipitated chlorophyll from the preliminary extract by vacuum concentration, concentrate to 230 g and filter to remove impurities such as chlorophyll, and finally obtain 180 g of pandan concentrate rich in phytol.

[0073] (VI) Purify phytol: Further purify the concentrate by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in the fields of food, cosmetics and medicine.

[0074] (VII) Determination of antioxidant capacity: Mix the purified phytol solution with the FRAP working solution in a certain ratio and react at a suitable temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed by the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0075] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0076] Comparative example: The concentrated solution was purified by using the traditional extraction and separation method instead of the chromatographic separation method, and other conditions remained unchanged. It was found that the calculated FRAP value was significantly smaller than the value calculated by the extraction with alcohol solution.

[0077] Example 5:

[0078] (I) Pretreatment: 400 g of fresh or dried pandan leaves were selected as raw materials, washed, dried and crushed to obtain pandan leaf powder with a modulus of 80. The powder was soaked in 900 g of distilled water and taken out after standing for 24 hours.

[0079] (II) Enzymatic hydrolysis of cellulose: The soaked pandan leaves were soaked in a solution containing 1% xylanase, glutathione peroxidase, papain and ficin, the temperature was controlled at 37 °C, and the reaction time was 2 hours.

[0080] (III) Grape seed extract: 1000 g of raw grape seeds were weighed, 4 times the amount of water was added, and stirring extraction was carried out at 100 °C for 3 times, 2 hours each time. The filtrate was obtained by filtering with a 200-mesh filter cloth. The filtrate was adsorbed by 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain grape seed extract;

[0081] (IV) Extraction with alcohol solution: The enzymolysis residue, grape seed extract and 900 g of ethanol solvent with a concentration of 60% were mixed in a ratio of 1:1, stirred evenly and then left standing for 2 hours. The filter residue was filtered, and the filtrate was collected and subjected to vacuum concentration treatment to obtain a preliminary extract.

[0082] (V) Concentrating to remove chlorophyll: Most of the solvent and precipitated chlorophyll were removed from the preliminary extract by vacuum concentration, concentrated to 300 g, and impurities such as chlorophyll were removed by filtration. Finally, 160 g of pandan concentrated solution rich in phytol was obtained.

[0083] (VI) Purifying phytol: The concentrated solution was further purified by using a chromatographic separation method to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in the fields of food, cosmetics and medicine, etc.

[0084] (VII) Determination of antioxidant capacity: The purified phytol solution is mixed with the FRAP working solution in a certain proportion and reacted at an appropriate temperature for a certain period of time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0085] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0086] Control example: When 900 g of water is used to replace the alcohol solution for extraction and other conditions remain unchanged, it will be found that the calculated FRAP value is significantly smaller than the value calculated by extracting with the alcohol solution.

[0087] Example 6 of implementation

[0088] (I) Pretreatment: Select 500 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. Soak the powder in 1000 g of distilled water and take it out after standing for 24 hours.

[0089] (II) Enzymatic hydrolysis of cellulose: Soak the soaked pandan leaves in a solution containing 1% xylanase, glutathione peroxidase, papain, and ficin, control the temperature at 37°C, and the reaction time is 2 hours.

[0090] (III) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water and stir and extract at 100°C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0091] (IV) Alcohol solution extraction: Mix the enzymatic hydrolysis residue, grape seed extract with 1000 g of 60% ethanol solvent in a ratio of 1:1, stir evenly and then stand for 2 hours. Filter the residue, collect the filtrate and perform vacuum concentration treatment to obtain a preliminary extract.

[0092] (V) Concentration to Remove Chlorophyll: The preliminary extract is concentrated under vacuum to remove most of the solvent and precipitated chlorophyll, concentrated to 200 g, and impurities such as chlorophyll are removed by filtration. Finally, 0 g of pandan concentrate rich in phytol is obtained. The antioxidant capacity of a sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, i.e., the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0093] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0094] Comparative Example: The concentrate is purified by using the traditional extraction and separation method instead of the chromatographic separation method, and other conditions remain unchanged. It will be found that the calculated FRAP value is significantly smaller than the value calculated by the alcohol solution extraction.

[0095] Example 7:

[0096] (I) Pretreatment: Select 100 g of fresh or dried pandan leaves as raw materials, wash, dry, and crush them to obtain pandan leaf powder with a modulus of 80. The powder is soaked in 800 g of distilled water and taken out after standing for 24 hours.

[0097] (II) Enzymatic Hydrolysis of Cellulose: The soaked pandan leaves are soaked in a solution containing 1% xylanase, 1% cellulase, glutathione peroxidase, papain, and ficin. The temperature is controlled at 37 °C, and the reaction time is 2 hours.

[0098] (III) Grape Seed Extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water, stir and extract at 100 °C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. The filtrate is adsorbed by 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0099] (IV) Alcohol Solution Extraction: The enzymolysis residue, grape seed extract, and 800 g of ethanol solvent are mixed in a ratio of 1:1, stirred evenly, and then left to stand for 2 hours. Filter the residue, collect the filtrate, and perform vacuum concentration to obtain a preliminary extract.

[0100] (V) Concentration to Remove Chlorophyll: The preliminary extract is concentrated under vacuum to remove most of the solvent and precipitated chlorophyll, concentrated to 300 g, and impurities such as chlorophyll are removed by filtration. Finally, 150 g of pandan concentrate rich in phytol is obtained.

[0101] (6) Purification of phytol: The concentrated solution is further purified by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine and other fields.

[0102] (7) Determination of antioxidant capacity: The purified phytol solution is mixed with the FRAP working solution in a certain proportion and reacted at an appropriate temperature for a certain period of time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced by per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0103] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0104] Control example: Use 800 g of water to replace the alcohol solution for extraction, and keep other conditions unchanged. It will be found that the calculated FRAP value is significantly smaller than the value calculated by extracting with the alcohol solution.

[0105] Example 8:

[0106] (1) Pretreatment: Select 200 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. Immerse the powder in 1200 g of distilled water, and take it out after standing for 24 hours.

[0107] (2) Enzymatic hydrolysis of cellulose: Immerse the soaked pandan leaves in a solution containing 1% xylanase, 1% cellulase, glutathione peroxidase, papain and ficin, control the temperature at 37 °C, and the reaction time is 2 hours.

[0108] (3) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water and stir and extract at 100 °C for 3 times, 2 hours each time. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0109] (4) Alcohol solution extraction: Mix the enzymatic hydrolysis residue, grape seed extract with 1200 g of 80% ethanol solvent in a ratio of 1:1, stir evenly and then stand for 2 hours. Filter the residue, collect the filtrate and perform vacuum concentration treatment to obtain a preliminary extract.

[0110] (V) Concentrating and Removing Chlorophyll: The preliminary extract is concentrated by vacuum to remove most of the solvent and the precipitated chlorophyll, concentrated to 250 g, and impurities such as chlorophyll are removed by filtration. Finally, 100 g of pandan concentrate rich in phytol is obtained.

[0111] (VI) Purifying Phytol: The concentrate is further purified by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine and other fields.

[0112] (VII) Determination of Antioxidant Capacity: The purified phytol solution is mixed with the FRAP working solution in a certain proportion and reacted at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), resulting in the color of the solution changing from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0113] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0114] Comparative Example: When the concentrate is purified by the traditional extraction and separation method instead of the chromatographic separation method, and other conditions remain unchanged, it will be found that the calculated FRAP value is significantly smaller than the value calculated by the alcohol solution extraction.

[0115] Example 9:

[0116] (I) Pretreatment: Select 100 g of fresh or dried pandan leaves as raw materials, wash, dry and crush them to obtain pandan leaf powder with a modulus of 80. The powder is soaked in 1000 g of distilled water and taken out after standing for 24 hours.

[0117] (II) Enzymatic Hydrolysis of Cellulose: The soaked pandan leaves are soaked in a solution containing 1% xylanase, 1% cellulase, glutathione peroxidase, papain and ficin, the temperature is controlled at 37 °C, and the reaction time is 2 hours.

[0118] (III) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water, and stir and extract at 100 °C for 3 times, each time for 2 hours. Filter with a 200-mesh filter cloth to obtain a filtrate. Adsorb the filtrate through 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain a grape seed extract;

[0119] (IV) Ethanol solution extraction: Mix the enzymolysis residue, grape seed extract, and 1000 g of an 80% ethanol solvent in a ratio of 1:1, stir evenly, and let stand for 2 hours. Filter the residue and collect the filtrate for vacuum concentration to obtain a preliminary extract.

[0120] (V) Concentrate to remove chlorophyll: Remove most of the solvent and precipitated chlorophyll from the preliminary extract by vacuum concentration, concentrate to 100 g, and remove impurities such as chlorophyll by filtration to finally obtain 80 g of a pandan concentrate rich in phytol.

[0121] (VI) Purify phytol: Further purify the concentrate by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in the fields of food, cosmetics, and medicine.

[0122] (VII) Determination of antioxidant capacity: Mix the purified phytol solution with the FRAP working solution in a certain ratio and react at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), causing the color of the solution to change from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0123] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0124] Control example: Use 1000 g of water instead of the ethanol solution for extraction, and keep other conditions unchanged. It will be found that the calculated FRAP value is significantly smaller than the value calculated by ethanol solution extraction.

[0125] Example 10:

[0126] (I) Pretreatment: Select 100 g of fresh or dried pandan leaves as raw materials, wash, dry, and crush them to obtain pandan leaf powder with a modulus of 80. Soak the powder in 1000 g of distilled water and take it out after standing for 24 hours.

[0127] (II) Enzymatic hydrolysis of cellulose: Soak the soaked pandan leaves in a solution containing 1% xylanase, 1% cellulase, glutathione peroxidase, papain, and ficin. Control the temperature at 37°C and the reaction time at 2 hours to obtain the enzymatically hydrolyzed residue.

[0128] (III) Grape seed extract: Weigh 1000 g of raw grape seeds, add 4 times the amount of water, stir and extract at 100°C for 3 times, each time for 2 hours. Filter through a 200-mesh filter cloth to obtain the filtrate. Adsorb the filtrate with 1000 ml of DM21 resin at an adsorption flow rate of 1 BV / h to obtain the grape seed extract.

[0129] (IV) Alcohol solution extraction: Mix the enzymatically hydrolyzed residue, grape seed extract, and 1000 g of 90% ethanol solvent in a 1:1 ratio, stir evenly, and let stand for 2 hours. Filter the residue and collect the filtrate, then perform vacuum concentration to obtain the preliminary extract.

[0130] (V) Concentrate to remove chlorophyll: Remove most of the solvent and precipitated chlorophyll from the preliminary extract by vacuum concentration, concentrate to 100 g, and filter to remove impurities such as chlorophyll to finally obtain 50 g of pandan concentrate rich in phytol.

[0131] (VI) Purify phytol: Further purify the concentrate by chromatographic separation to remove impurities and improve the purity of phytol. The purified phytol can be used as an antioxidant in food, cosmetics, medicine, and other fields.

[0132] (VII) Determination of antioxidant capacity: Mix the purified phytol solution with the FRAP working solution in a certain ratio and react at an appropriate temperature for a certain time. During the reaction, the antioxidant component Fe(III) in the sample is reduced to Fe(II), causing the color of the solution to change from light yellow or colorless to blue-violet. By measuring the change in absorbance of the solution at a specific wavelength (such as 593 nm) before and after the reaction, the antioxidant capacity of the purified phytol can be calculated. The antioxidant capacity of the sample is usually expressed as the amount of Fe(III) that can be reduced per unit mass or volume of the sample, that is, the FRAP value. The calculation formula is as follows: FRAP value = (ΔC × V) / (m × Δt)

[0133] Where: ΔC is the change in the concentration of Fe2+ before and after the reaction; V is the total volume of the reaction system (including the sample and the working solution); m is the mass or volume of the sample; Δt is the reaction time; the higher the FRAP value, the stronger the antioxidant capacity.

[0134] Comparative example: The concentrated solution was purified by using the traditional extraction and separation method instead of the chromatographic separation method, and other conditions remained unchanged. It was found that the calculated FRAP value was significantly smaller than the value calculated by extraction with alcohol solution.

[0135] Please refer to the attached Figure 2 - attached Figure 6 As shown in the attached drawings, an apparatus for extracting phytol from pandan leaves provided by an embodiment of the present invention includes an extraction tank 1. A support leg 2 is fixed to the lower surface of the extraction tank 1, and a heating plate 14 is fixed to the outer wall of the extraction tank 1; a crushing assembly 3 is disposed on the extraction tank 1, and the crushing assembly 3 is used for crushing pandan leaves into fine particles. The crushing assembly 3 includes a hopper 301, the bottom end of the hopper 301 penetrates through the top end of the extraction tank 1, a connecting plate 302 is fixed to the top end of the extraction tank 1, a first motor 303 is fixed inside the connecting plate 302, a first rotating rod 304 is fixed to the output end of the first motor 303, a first gear 305 is fixed to the end of the first rotating rod 304, a second rotating rod 306 is rotatably disposed inside the hopper 301, a second gear 307 is fixed to the end of the second rotating rod 306, the second gear 307 meshes with the first gear 305, and crushing rollers 308 are fixed to the outer walls of the first rotating rod 304 and the second rotating rod 306; a stirring assembly 4 is disposed inside the extraction tank 1 for enhancing the contact between the extraction solvent and the pandan leaves.

[0136] The stirring assembly 4 includes a second motor 401, the outer wall of the second motor 401 is fixed to the top end of the extraction tank 1, a driving gear 402 is fixed to the driving end of the second motor 401, symmetrically left and right stirring rods 403 are rotatably disposed inside the extraction tank 1, a driven gear 404 is fixed to the end of the stirring rod 403, the driven gear 404 meshes with the driving gear 402, and a plurality of stirring blades 405 are fixed to the outer walls of the stirring rods 403.

[0137] A fixing plate 5 is fixed to the outer wall of the support leg 2, a delivery pump 6 is fixed to the upper surface of the fixing plate 5, a suction pipe 7 is fixed to the input end of the delivery pump 6, the end of the suction pipe 7 penetrates through the bottom end of the extraction tank 1, a delivery pipe 8 is fixed to the output end of the delivery pump 6, and the output end of the delivery pipe 8 is fixed to a filter tank 9.

[0138] A filter screen 10 is fixed inside the filter tank 9, a filter membrane 11 is fixed inside the filter tank 9, a discharge pipe 12 penetrates through the bottom end of the filter tank 9, and a valve 13 is disposed inside the discharge pipe 12.

[0139] A temperature sensor 15 penetrates through one side of the extraction tank 1, and a pressure sensor 16 penetrates through one side of the extraction tank 1 for monitoring the temperature and pressure changes in the extraction tank 1 during the extraction process in real time to ensure stable extraction conditions. A controller 17 is fixed to the outer wall of the extraction tank 1, and the controller 17 is electrically connected to the pressure sensor 16 and the temperature sensor 15.

[0140] Working principle: First, the crushing component 3 introduces pandan leaves into the extraction tank 1 through the hopper 301. Driven by the first motor 303, the first rotating rod 304 and the second rotating rod 306 are meshed with each other through the first gear 305 and the second gear 307, so that the crushing roller 308 performs crushing and cutting on the pandan leaves, crushing them into fine particles to enhance the contact surface between the pandan leaves and the extraction solvent. Subsequently, the crushed pandan leaves and an appropriate amount of extraction solvent such as alcohol enter the extraction tank 1 through the hopper 301. The stirring component 4 in the extraction tank 1 drives the stirring rod 403 to rotate through the second motor 401. The driven gear 404 of the stirring rod 403 is meshed with the driving gear 402, driving a plurality of stirring blades 405 to stir in the extraction tank 1 to ensure that the extraction solvent is fully mixed with the pandan leaves and improve the extraction efficiency of effective components such as phytol. At the same time, the heating plate 14 heats the solvent in the extraction tank 1 to increase the temperature and further accelerate the dissolution of phytol. After the extraction is completed, the extract is pumped by the delivery pump 6 through the extraction pipe 7 and sent into the filtration tank 9 through the delivery pipe 8. In the filtration tank 9, the filter screen 10 and the filter membrane 11 work together to remove solid impurities, ensuring that the pure phytol extract flows into the discharge pipe 12 and finally flows out through the control of the valve 13. To ensure the stability during the extraction process, the temperature sensor 15 and the pressure sensor 16 monitor the temperature and pressure in the extraction tank 1 in real time and transmit the data to the controller 17 to automatically adjust the extraction conditions and ensure the stable progress of the extraction process.

[0141] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirits of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for preparing a phytol antioxidant from Paniculata var. The following steps are involved: Pretreatment: fresh or dried pandan leaves are selected as raw materials, and washed, dried and crushed to obtain pandan leaf powder with a modulus of 80; Enzymatic hydrolysis of cellulose: Mix the pandan leaf powder with water in a ratio of 1:2 to 1:20, and add an appropriate amount of one or more of cellulose enzyme, cellobiase, xylanase, glucan sucrase, fructase, glutathione peroxidase, papain, and fig enzyme for enzymatic hydrolysis; Alcohol solution extraction: soak the enzymatic residue in an ethanol solution with a concentration of 30% to 95%, and heat and reflux extract at a temperature of 65°C to 73°C for 5 hours to obtain a colorful extract; Concentration and removal of chlorophyll: The pandan extract is vacuum concentrated to remove most of the solvent and precipitated chlorophyll to obtain a concentrated solution; Purification: Use chromatographic separation technology to purify the concentrate to obtain phytol with higher purity.

2. The method for preparing the pandanus phytol antioxidant according to claim 1, characterized in that: The method includes an antioxidant capacity determination, wherein a purified phytol solution is mixed with a FRAP working solution in a certain proportion, and reacted at a suitable temperature, and the antioxidant component Fe(III) in the sample is reduced to Fe(II), causing the solution color to change from light yellow or colorless to bluish purple. By determining the absorbance change of the solution at a specific wavelength of 593 nm before and after the reaction, the antioxidant capacity of the purified phytol can be calculated.

3. The method for preparing the pandanus phytol antioxidant according to claim 1, characterized in that: In the enzymatic hydrolysis step, the enzyme addition amount is 0.1% to 2% of the substrate, the enzymatic hydrolysis temperature is controlled between 35°C and 75°C, and the enzymatic hydrolysis time is 2 hours to 8 hours. In the alcohol solution extraction step, the ethanol solution concentration is 30% to 95%, the heating temperature is controlled between 65°C and 73°C, and the extraction time is 5 hours.

4. The method for preparing the pandanus phytol antioxidant according to claim 1, characterized in that: The concentration step uses vacuum concentration technology to reduce the loss of heat-sensitive components, and further removes chlorophyll by filtration to obtain a concentrated solution rich in phytol.

5. The method for preparing the pandanus leaf-based phytol antioxidant according to claim 1, characterized in that: The purification step uses chromatography separation technology to remove impurities and improve the purity of phytol.

6. A device for extracting phytol from Pandan leaves, applied to the method for preparing the Pandan leaf-based phytol antioxidant according to claims 1-5, characterized in that: The invention comprises an extraction tank (1), a support leg (2) being fixed to the lower surface of the extraction tank (1), a heating plate (14) being fixed to the outer wall of the extraction tank (1); a crushing assembly (3) being arranged on the extraction tank (1), the crushing assembly (3) being used to crush the pandan leaves into fine particles, the crushing assembly (3) comprising a hopper (301), the bottom end of the hopper (301) passing through the top end of the extraction tank (1), a connecting plate (302) being fixed to the top end of the extraction tank (1), a motor (303) being fixed inside the connecting plate (302), the motor A rotating rod 1 (304) is fixed to the output end of the first (303), a gear 1 (305) is fixed to the end of the rotating rod 1 (304), a rotating rod 2 (306) is rotated inside the hopper (301), a gear 2 (307) is fixed to the end of the rotating rod 2 (306), the gear 2 (307) and the gear 1 (305) are meshed, and a crushing roller (308) is fixed to the outer wall of the rotating rod 1 (304) and the rotating rod 2 (306); a stirring assembly (4) is arranged in the extraction tank (1) and is used to enhance the contact between the extraction solvent and the colorful leaves.

7. The device for extracting phytol from Pandan leaves according to claim 6, characterized in that: The stirring assembly (4) comprises a second motor (401), the outer wall of the second motor (401) is fixed to the top of the extraction tank (1), a driving gear (402) is fixed to the driving end of the second motor (401), a left-right symmetrical stirring rod (403) is rotated inside the extraction tank (1), a driven gear (404) is fixed to the end of the stirring rod (403), the driven gear (404) and the driving gear (402) are meshed, and a plurality of stirring blades (405) are fixed to the outer wall of the stirring rod (403).

8. The device for extracting phytol from Pandan leaves according to claim 6, characterized in that: A fixing plate (5) is fixed to the outer wall of the support leg (2), a delivery pump (6) is fixed to the upper surface of the fixing plate (5), an extraction pipe (7) is fixed to the input end of the delivery pump (6), the end of the extraction pipe (7) passes through the bottom end of the extraction tank (1), a delivery pipe (8) is fixed to the output end of the delivery pump (6), and a filter tank (9) is fixed to the output end of the delivery pipe (8).

9. The device for extracting phytol from Pandan leaves according to claim 8, characterized in that: A filter screen (10) is fixed inside the filter tank (9), a filter membrane (11) is fixed inside the filter tank (9), a discharge pipe (12) runs through the bottom end of the filter tank (9), and a valve (13) is arranged inside the discharge pipe (12).

10. The device for extracting phytol from Pandan leaves according to claim 6, characterized in that: A temperature sensor (15) is passed through one side of the extraction tank (1), and a pressure sensor (16) is passed through one side of the extraction tank (1), which are used to monitor the temperature and pressure changes in the extraction tank (1) in real time during the extraction process to ensure that the extraction conditions are stable. A controller (17) is fixed to the outer wall of the extraction tank (1), and the controller (17) is electrically connected to the pressure sensor (16) and the temperature sensor (15).

Images