Preparation process and use method of pure natural freckle-removing whitening liquid

Abstract

The invention discloses a preparation process and a use method of a pure natural freckle-removing whitening lotion, relates to the field of material science, and solves the problems of unstable effect, insufficient extraction of active ingredients, limited storage life, single formula and high production cost. The preparation process comprises the following steps: firstly, preparing raw materials and a reaction device of the pure natural freckle-removing whitening lotion; the preparation method comprises the following steps: preparing nicotinamide by adopting a protein hydrolysis method, extracting fruit essence, glycyrrhizic acid, tea polyphenol and lavender essential oil by adopting a microwave high-performance extraction instrument, preparing vitamin C by adopting a bioreactor, extracting vitamin E by adopting a cold pressing technology and the microwave high-performance extraction instrument, and fermenting hyaluronic acid by adopting a solid fermentation technology. The pure natural freckle-removing whitening lotion and the preparation method thereof have the advantages that the production efficiency and the product quality of the preparation method can be improved, and pollution in the production process is reduced.

Description

Technical Field

[0001] The invention relates to the field of material science, and more specifically to a preparation process and a use method of a pure natural freckle removing and whitening liquid. Background Art

[0002] Pure natural freckle removal and whitening liquid is a product that combines freckle removal, whitening, moisturizing and skin care. Its unique pure natural formula and remarkable effect make it popular among consumers in the market. It can not only effectively lighten spots and brighten the skin tone, but also restore the skin to a healthy and youthful state. Its all-natural ingredients are very safe to use and will not cause any harm to the skin. This product mainly targets skin spots, such as sun spots, chloasma and freckles, etc. It inhibits melanin synthesis and metabolism, lightens spots, and makes the skin brighter and more even. In addition to freckle removal and whitening, it can also be used as part of daily skin care. It can be used every morning and evening to provide the skin with sufficient nutrition and moisturizing, so that the skin remains hydrated and healthy. Therefore, the development of an environmentally friendly, healthy, pure natural and side-effect-free freckle removal and whitening product is of great significance to meet market demand and improve product competitiveness.

[0003] However, although the traditional method of preparing pure natural freckle removing and whitening liquid emphasizes the use of natural ingredients, it still has problems such as unstable effect, insufficient extraction of active ingredients, limited shelf life, single formula and high production cost. Due to the imprecision of traditional preparation process and imperfect quality control, the effect of pure natural freckle removing and whitening liquid is unstable. When extracting the effective active ingredients of the raw materials, the content of active ingredients in the product is insufficient, which affects the freckle removing and whitening effect of the product. Since pure natural ingredients are easy to oxidize and deteriorate, the traditionally prepared freckle removing and whitening liquid usually has a short shelf life, which limits the circulation of the product and increases the risk of consumer purchase and use.

[0004] Therefore, the present invention discloses a preparation process and a method for using a pure natural freckle removing and whitening liquid. The preparation process is simple, convenient and efficient, and a pure natural freckle removing and whitening liquid with obvious effects and stable properties can be obtained, which has high commercialization and industrial production potential. Summary of the invention

[0005] Aiming at the deficiencies of the prior art, the present invention discloses a preparation process and a usage method of a pure natural freckle-removing and whitening liquid. The preparation process is simple, convenient and efficient, and a pure natural freckle-removing and whitening liquid with obvious effects and stable properties can be obtained, which has high potential for commercialization and industrial production. By using a quantum optimization algorithm for presetting and optimization, accurate control of the raw material ratio can be achieved, the product quality and production efficiency are improved, and the preparation process is made more efficient and cost-saving. At the same time, the use of a microwave high-efficiency extraction instrument to achieve automatic extraction greatly reduces the difficulty and labor intensity of manual operation, and also ensures the uniformity and high efficiency of the reaction, making the reaction process more stable and uniform. Using a steady-state melting machine makes the mixing of the preparation raw materials more sufficient and uniform, and the reaction time is shorter, thus improving the production capacity. In addition, the addition of various raw materials such as niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, etc. makes the effects of the pure natural freckle-removing and whitening liquid more stable, the formula more diverse, the shelf life longer and the cost lower.

[0006] The present invention adopts the following technical solutions: A preparation method of a pure natural freckle-removing and whitening liquid, characterized in that: the preparation method includes: Step 1, prepare the raw materials for the preparation of the pure natural freckle-removing and whitening liquid and the reaction device. The raw materials for the preparation of the pure natural freckle-removing and whitening liquid include niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, citric acid, glycerol and pure water. The niacinamide, vitamin C, vitamin E and hyaluronic acid are all prepared from natural substances. The ratio of the raw materials is preset and optimized by using a quantum optimization algorithm model. The reaction device uses a microwave high-efficiency extraction instrument to achieve automatic extraction. The quantum optimization algorithm model includes an initialization module, a quantum evolution module, an observation and reading module, and an update and decision module; The initialization module is the starting point of the quantum optimization algorithm model, determines the coding method, improves the parallelism, and provides a basis for subsequent quantum operations; The quantum evolution module realizes the manipulation of the quantum bit state through the operation of the Hadamard gate or the phase flip gate, and through designing a suitable Hamiltonian operator, makes the system evolve towards a lower energy and better solution direction; The observation and reading module is the process of converting quantum information into classical information. By repeating the experiment multiple times and statistically analyzing the probability distribution of the results, the position of the optimal solution is estimated; The update and decision module updates the solution to the problem according to the observation results and makes a decision on whether to continue iteration; Step 2, extract aspartic acid from soybeans by the protein hydrolysis method, and then sequentially add aspartic acid oxidase, quinolinic acid phosphoribosyltransferase and nicotinic acid mononucleotide enzyme to obtain the niacinamide. The reaction formula for preparing the niacinamide is: In formula (1), is the aspartic acid, is the niacinamide, and H2O is a water molecule; Step 3: Add the pulverized bearberry leaf powder, licorice powder, lavender powder, and ethanol to a microwave high-efficiency extractor respectively. Input the type and state of the sample into the intelligent control system of the microwave high-efficiency extractor. The microwave high-efficiency extractor automatically adjusts the microwave power and irradiation time. Finally, take out the sample, and use high-performance liquid chromatography technology to respectively obtain the arbutin with a purity of 99%, the glycyrrhizic acid with a purity of 99%, the tea polyphenols with a purity of 99%, and the lavender essential oil with a purity of 99% from the sample; Step 4: Put the squeezed citrus fruit juice into a bioreactor filled with microalgae organisms. Control the temperature of the bioreactor to be 27 - 31°C and the pH to be 4.7 - 5.4. By continuously introducing oxygen with a volume fraction of 21%, the microalgae organisms in the bioreactor convert the fruit juice into dehydroascorbic acid, and then add a converting enzyme to reduce the dehydroascorbic acid into the vitamin C. Finally, perform final purification on the vitamin C through microfiltration membrane filtration, ion exchange resin adsorption, and high-performance liquid chromatography technology to obtain the vitamin C with a purity of 99%. The equation for the conversion of dehydroascorbic acid into vitamin C is: In formula (2), is the dehydroascorbic acid, is the vitamin C; Step 5: Treat wheat germ by cold pressing technology to obtain wheat germ oil rich in tocotrienols. Then add the wheat germ oil to a microwave high-efficiency extractor to extract the tocotrienols. Then add an enzyme preparation, cyclooxygenase, control the reaction temperature to be 37°C and the time to be 12 h, and convert the tocotrienols into the vitamin E. The equation for the conversion of tocotrienols into vitamin E is: In formula (3), is the tocotrienol, is the vitamin E; Step 6: Add the Streptococcus strain to a culture medium containing 65% glucose, 34% yeast powder, a culture medium with a concentration of 4 ml / L, a biostimulant made from plant extracts, and trace elements 0.5% zinc, 0.3% manganese, 0.1% sodium, and 0.1% selenium. Control the temperature to be 37°C, the pH to be 7.0, and the oxygen concentration to be 2%. After fermentation for 12 h, collect the fermentation product containing a large amount of the hyaluronic acid, and obtain the hyaluronic acid with a purity of 99% through continuous centrifugation and filtration systems and by using ethanol precipitation; Step 7: Mix the niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, glycerol and pure water according to a preset ratio, adjust the pH to 5.5 - 6.5 with the citric acid, add the mixture to the mixing tank of a steady-state melting machine, control the rotation speed at 5000 - 11000 r / min for high-speed shear mixing for 0.5 h, and then adjust the rotation speed to 3000 r / min for fine shear for 2 h until the uniform and delicate natural freckle-removing and whitening liquid is formed. As a further technical solution of the present invention, the quantum optimization algorithm model establishes a correlation function between the ratio of the preparation raw materials and the performance indexes of the natural freckle-removing and whitening liquid, and the sample set composed of the preparation raw materials is The expression of the correlation function is: In formula (4), F is the performance index of the natural freckle-removing and whitening liquid, M and N are weights, is the ratio of the preparation raw materials, i is the ordinal number of the preparation raw materials, a is an element in U, p is an element in U, Finally, use the evaluation criterion to evaluate whether the ratio of the preparation raw materials can synthesize the optimal performance index of the natural freckle-removing and whitening liquid. The correlation function of the evaluation criterion is: In formula (5), is the evaluation criterion, C is the label set, is the information of the ratio of the preparation raw materials, is the evaluation criterion of the preparation raw material coordinates. When the performance index of the natural freckle-removing and whitening liquid is optimal; The implementation process of the quantum optimization algorithm is as follows: First, initialize the ratio of the preparation raw materials and the performance indexes of the natural freckle-removing and whitening liquid, determine an initial quantum state, then construct the Hamiltonian of the system according to the correlation function, then use quantum gate operations to evolve the initial quantum state, measure the evolved quantum state to obtain a set of candidate solutions for the ratio of the preparation raw materials, and finally convert the measurement results into the actual ratio of the preparation raw materials and calculate the corresponding performance index F of the natural freckle-removing and whitening liquid. As a further technical solution of the present invention, the mass parts of the preparation raw materials include 15 mass parts of niacinamide, 13 mass parts of arbutin, 11 mass parts of glycyrrhizic acid, 9 mass parts of tea polyphenols, 19 mass parts of lavender essential oil, 2 mass parts of vitamin C, 1.3 mass parts of vitamin E, 5 mass parts of hyaluronic acid, 0.9 mass parts of citric acid, 8 mass parts of glycerol and 100 mass parts of pure water.

[0007] As a further technical solution of the present invention, the working method of the quantum evolution module in the first step is as follows: By analyzing the state and evolution history of the quantum system in real time, dynamically adjusting the parameters of the quantum gates, determining the "hot spot" areas of the search space by monitoring the entropy of the quantum state distribution, and correspondingly enhancing the search accuracy in these areas. This adaptive strategy is expressed by the formula: In formula (6), is the quantum gate operation at time step t, and f is the adjustment function obtained according to the entropy of the system state obtained, is the basic quantum gate operation. In this way, we can ensure that the evolution of the quantum system has sufficient randomness for global search and clear direction for local fine exploration. As a further technical solution of the present invention, the working method of the protein hydrolysis method in the second step is as follows: Soak the selected soybeans in a buffer solution with a pH of 7.0. The buffer solution is a citric acid and sodium hydroxide buffer system with a molar ratio of 3:1. Add 10% of the hydrolysis initiation enzyme trypsin, and hydrolyze at a constant temperature of 30°C for 1 h. Then, sequentially add 5% of the aspartate oxidase, 4.5% of the quinolinic acid phosphoribosyltransferase, and 3% of the nicotinic acid mononucleotide enzyme to obtain the nicotinamide.

[0008] As a further technical solution of the present invention, the working method of the high-performance liquid chromatography technology in the third step is as follows: Fill the chromatographic column filler with silica with a particle size of 8 microns and an assist value of 100,000 / m. Control the temperature at 25°C and the pressure at 101.3 Kpa. Flow the solution containing the arbutin through the chromatographic column to specifically absorb the arbutin and elute other components. Subsequently, use an ethanol eluent to elute and collect the arbutin to obtain the arbutin with a purity of 99%.

[0009] As a further technical solution of the present invention, the microwave high-efficiency extraction instrument includes a microwave generator, a microwave transmission system, a sample container, a solvent system, a control system, a collection system, and a cooling system. The microwave generator consists of a magnetron and is located at the center of the entire microwave high-efficiency extraction instrument. The microwave transmission system is connected to the sample container in a through connection by welding. The microwave transmission system is connected to the microwave generator in a through connection by welding. The solvent system is located on the right side of the sample container by welding, adding or circulating a specific solvent to the sample container. The control system is welded to the upper end of the microwave generator to control the power of the microwave generator and monitor parameters such as the temperature, pressure, and time of the entire extraction process. The collection system is welded to the end of the entire device, and the cooling system is located around the sample container by welding.

[0010] As a further technical solution of the present invention, the steady-state fine fusion machine includes an energy input unit, a stability control module, a fine fusion chamber and an output port. The energy input unit is connected to the outside of the entire device by a circuit to convert electrical energy into thermal energy. The stability control module is connected to the fine fusion chamber in the form of a circuit connection to monitor the operating status of the sample in the fine fusion chamber and control the temperature, pressure and stability of the energy input in real time. The fine fusion chamber and the output port are connected through a pipeline in a welding manner.

[0011] As a further technical solution of the present invention, a method for using a pure natural freckle removing and whitening liquid is provided, and the method for using the pure natural freckle removing and whitening liquid comprises the following steps: Step 1. Clean the skin. After moistening the face with warm water, take a soybean-sized amount of mild facial cleanser and gently massage the entire face to thoroughly remove the oil and dirt on the skin surface and then wash it off with clean water; use a handheld multi-spectral skin analyzer with a wavelength range of 400-1100nm to scan the face and generate a skin spot distribution heat map with a resolution of 0.1mm² / pixel in real time; Step 2: Based on the convolutional neural network (CNN) algorithm module, freckles, chloasma, and post-inflammatory pigmentation are identified, and the percentage of pigmentation area is calculated with an accuracy of ±1.2%. The data is synchronized to the mobile terminal APP via Bluetooth to generate a personalized usage plan. Pat the skin with a towel until it is semi-dry, and maintain a certain degree of moisture to allow the active ingredients to penetrate better. When the percentage of pigmentation area is greater than 15%, the targeted penetration mode is activated: the whitening solution is loaded onto a microneedle patch with a needle length of 150μm and a density of 200 needles / cm², and the active ingredients are delivered in a targeted manner through a programmable microcurrent permeation enhancer with a current intensity of 0.3mA / cm² and a frequency of 10Hz. When the area of ​​pigmentation is ≤15%, the photodynamic synergy mode is activated: with an LED phototherapy device with a wavelength of 415nm and an irradiance of 50mW / cm², the vitamin C derivatives in the whitening solution are activated; Step 3: Take a rice-sized amount of the pure natural freckle-removing and whitening liquid and perform a local applicability test behind the ear to ensure safety and no allergic reaction; the whitening liquid adopts a thermosensitive liposome encapsulation technology with a phase transition temperature of 32°C. When the liposome contacts the skin, it ruptures and releases the active substance; The integrated 50μm-thick flexible electronic skin patch has a built-in pH sensor with an accuracy of ±0.1 and a humidity sensor with an accuracy of ±3%RH to monitor the skin microenvironment in real time: When pH>6.0 is detected, the sustained-release system is triggered to extend the release time of arbutin; When the humidity is less than 60%RH, the gradient release mechanism of hyaluronic acid is activated; Step 4: First, squeeze out one pump and apply it evenly on the whole face. Then, squeeze out another pump and apply it locally on the areas with severe spots. Massage with the intelligent piezoelectric massage head, which monitors the skin absorption status in real time through impedance analysis with a frequency of 1 MHz and a sensitivity of 0.1 Ω. The massage time is 5 - 10 minutes. Step 5: After use, spray a bioactive protective film. The film-forming material is a silk fibroin - dextran complex with a mass ratio of 7:3, and the oxygen permeability rate is ≥500 cm³ / (m²·d).

[0012] The present invention discloses a preparation process and usage method of a pure natural freckle-removing and whitening liquid. The preparation process is simple, convenient, and efficient, and a pure natural freckle-removing and whitening liquid with obvious effects and stable properties can be obtained, having high potential for commercialization and industrial production. By using the quantum optimization algorithm model for presetting and optimization, accurate control of the raw material ratio can be achieved, improving the product quality and production efficiency, making the preparation process more efficient and cost-saving. At the same time, using a microwave high-efficiency extraction instrument to achieve automatic extraction greatly reduces the difficulty and labor intensity of manual operation, and also ensures the uniformity and high efficiency of the reaction, making the reaction process more stable and uniform. Using a steady-state melting machine makes the mixing of the preparation raw materials more sufficient and uniform, with a shorter reaction time, thus improving the production capacity. In addition, adding various raw materials such as niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, and lavender essential oil makes the effects of the pure natural freckle-removing and whitening liquid more stable, the formula more diverse, the shelf life longer, and the cost lower. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 It is a flow architecture diagram of a preparation method of a pure natural freckle-removing and whitening liquid of the present invention; Figure 2 It is a schematic diagram of the content proportion of the preparation raw materials in a preparation method of a pure natural freckle-removing and whitening liquid of the present invention; Figure 3 It is an architecture diagram of a microwave high-efficiency extraction instrument in a preparation method of a pure natural freckle-removing and whitening liquid of the present invention; Figure 4 It is an architecture diagram of a steady-state melting machine in a preparation method of a pure natural freckle-removing and whitening liquid of the present invention. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0014] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention. Embodiment

[0015] A preparation method of a pure natural freckle-removing and whitening liquid, wherein the preparation method comprises: Step 1, Prepare the raw materials and reaction device for the preparation of the pure natural freckle-removing and whitening liquid. The raw materials for the preparation of the pure natural freckle-removing and whitening liquid include niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, citric acid, glycerol and pure water. The niacinamide, vitamin C, vitamin E and hyaluronic acid are all prepared from natural substances. The ratio of the raw materials is preset and optimized by a quantum optimization algorithm model. The reaction device uses a microwave high-efficiency extraction instrument to achieve automatic extraction. The quantum optimization algorithm model includes an initialization module, a quantum evolution module, an observation and reading module, and an update and decision module; The initialization module is the starting point of the quantum optimization algorithm model, determines the encoding method, improves parallelism, and provides a basis for subsequent quantum operations; The quantum evolution module realizes the manipulation of the quantum bit state through the operation of Hadamard gates or phase flip gates, and designs a suitable Hamiltonian operator to make the system evolve in the direction of lower energy and better solution; The observation and reading module is the process of converting quantum information into classical information. By repeating experiments multiple times and statistically analyzing the probability distribution of the results, the position of the optimal solution is estimated; The update and decision module updates the solution to the problem according to the observation results and makes a decision on whether to continue iteration; Step 2, Extract aspartic acid from soybeans by the protein hydrolysis method, and then sequentially add aspartic acid oxidase, quinolinic acid phosphoribosyltransferase and nicotinic acid mononucleotide enzyme to obtain the niacinamide. The reaction formula for preparing the niacinamide is: In formula (1), is the aspartic acid, is the niacinamide, and H2O is a water molecule; Step 3, Add the pulverized arbutus leaf powder, licorice powder, lavender powder and ethanol to the microwave high-efficiency extraction instrument respectively. Input the type and state of the sample into the intelligent control system of the microwave high-efficiency extraction instrument. The microwave high-efficiency extraction instrument automatically adjusts the microwave power and irradiation time, and finally takes out the sample. Use high-performance liquid chromatography technology to obtain the arbutin with a purity of 99%, the glycyrrhizic acid with a purity of 99%, the tea polyphenols with a purity of 99% and the lavender essential oil with a purity of 99% from the sample respectively; Step 4: Put the squeezed citrus fruit juice into a bioreactor filled with microalgae organisms, control the temperature of the bioreactor to be 27 - 31 °C and the pH to be 4.7 - 5.4. By continuously introducing oxygen with a volume fraction of 21%, the microalgae organisms in the bioreactor convert the fruit juice into dehydroascorbic acid. Then, add invertase to reduce the dehydroascorbic acid to the vitamin C. Finally, perform final purification on the vitamin C through microfiltration membrane filtration, ion exchange resin adsorption, and high-performance liquid chromatography technology to obtain the vitamin C with a purity of 99%. The equation for the conversion of dehydroascorbic acid to the vitamin C is: In formula (2), is the dehydroascorbic acid, is the vitamin C; Step 5: Treat wheat germ using cold pressing technology to obtain wheat germ oil rich in tocotrienols. Then, add the wheat germ oil to a microwave high-efficiency extractor to extract the tocotrienols. Next, add the enzyme preparation cyclooxygenase, control the reaction temperature to be 37 °C and the time to be 12 h, and convert the tocotrienols to the vitamin E. The equation for the conversion of tocotrienols to the vitamin E is: In formula (3), is the tocotrienol, is the vitamin E; Step 6: Add Streptococcus strains to a culture medium containing 65% glucose, 34% yeast powder, a culture medium with a concentration of 4 ml / L, a biostimulant made from plant extracts, and trace elements 0.5% zinc, 0.3% manganese, 0.1% sodium, and 0.1% selenium. Control the temperature to be 37 °C, the pH to be 7.0, and the oxygen concentration to be 2%. After fermentation for 12 h, collect the fermentation product containing a large amount of the hyaluronic acid, and obtain the hyaluronic acid with a purity of 99% through continuous centrifugation and filtration systems and ethanol precipitation; Step 7: Mix the niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, glycerol, and pure water according to a preset ratio, adjust the pH to 5.5 - 6.5 with citric acid, add the mixture to the mixing tank of a steady-state melting machine, control the rotation speed to be 5000 - 11000 r / min for high-speed shear mixing for 0.5 h, and then adjust the rotation speed to 3000 r / min for fine shear for 2 h until the uniform and delicate natural freckle-removing and whitening liquid is formed. In a further embodiment, introduce a quantum-classical hybrid learning framework into the quantum optimization algorithm model, including: Quantum feature mapping module: Encode the raw material ratio parameters into the quantum state ψ(θ)>=U(θ)∣0>ψ(θ)>=U(θ)∣0>, where U(θ) is a parameterized quantum circuit with a depth ≤ 10 layers; Classical Reinforcement Learning Module: Construct a Deep Q-Network (DQN) that takes the quantum evolution result as the state input and outputs the optimal process parameter adjustment strategy; Dynamic Feedback Mechanism: Update the quantum circuit parameter θ through the HPLC component analysis data obtained in real time (sampling interval ≤ 5 min) to form a closed-loop optimization system.

[0016] In Step 3, the microwave high-efficiency extraction instrument is upgraded to a microwave-subcritical water co-extraction system. The technical features include: Subcritical water pretreatment: Before microwave irradiation, pretreat the plant powder with subcritical water at 100 - 150 °C (pressure 1 - 2 MPa) for 10 - 15 min to expand and rupture the cell wall; Dynamic Solvent Gradient: The extraction solvent uses an ethanol / CO2 supercritical fluid (critical temperature 31 °C, pressure 7.4 MPa) mixed system, and the volume ratio is gradually adjusted from 5:95 to 40:60; Online monitoring: Integrate a near-infrared spectroscopy probe (wavelength 1200 - 2400 nm) to monitor the target component concentration in real time, and automatically terminate the extraction when the detected arbutin concentration ≥ 98.5%.

[0017] The microalgae in Step 4 is an engineered Scenedesmus obliquus mutant SO-HY5 edited by CRISPR-Cas9, and its technical features include: Overexpress L-galactose dehydrogenase (GALDH, EC 1.1.1.117), and the activity is increased by 4.8 times; Knock out the gene of dehydroascorbate reductase (DHAR, EC 1.8.5.1) to block the reverse metabolic pathway; Under the conditions of 27 °C and pH 5.0, the vitamin C synthesis rate reaches 2.3 g / L·h, which is 5.6 times higher than that of the wild type.

[0018] In a specific embodiment, the immobilization process of cyclooxygenase in Step 5 includes: Preparation of magnetic nanoparticles: Synthesize Fe3O4@ZIF-8 core-shell structure (shell thickness 20 ± 3 nm) by the microemulsion method, and the specific surface area ≥ 1200 m² / g; Directed immobilization technology: Specifically bind through the His tag and the Ni-NTA group on the carrier surface, and the enzyme loading amount ≥ 350 mg / g; Magnetic separation circulation device: Set a gradient magnetic field (0.5 - 1.2 T) to achieve rapid recovery of the catalyst within 10 seconds, and the activity retention rate ≥ 90% after 15 cycles of use.

[0019] In Step 6, the fermentation system is upgraded to a metabolic flow dynamic regulation system. The technical features include: Online metabolite analysis: The concentration of hyaluronic acid precursor (UDP-N-acetylglucosamine) is monitored in real time using a micro mass spectrometry probe (m / z 50 - 1000, resolution ≤ 0.1 Da); Adaptive feeding strategy: When the detected glucose concentration < 15 g / L, a pulsed feeding pump is started (single feeding volume ≤ 0.5 mL) to maintain the carbon-nitrogen ratio at 2.3 - 2.7; Quorum sensing intervention: An AI-2 type autoinducer is added (concentration 10 - 100 nM) to synchronize the bacterial growth cycle and make the hyaluronic acid production volatility < 5%.

[0020] Nanostructuring treatment is added before the mixing process in step seven: Liposome coating: Vitamin C, arbutin, and hydrogenated lecithin (HSPC:cholesterol = 7:3) are made into nano-liposomes with a particle size of 80 - 120 nm, and the encapsulation efficiency ≥ 95%; Liquid crystal phase construction: Monolein (GMO) and F127 block copolymer (mass ratio 3:1) are added to form a cubic liquid crystal carrier to load vitamin E and tea polyphenols; Targeted modification: A cell-penetrating peptide (TAT, sequence YGRKKRRQRRR) is grafted onto the surface of the carrier to increase the epidermal permeability by 3.2 times.

[0021] A zero-emission recycling system is achieved throughout the preparation process: Solvent recovery: After the microwave extraction waste liquid is adsorbed by molecular sieves and vacuum distilled, the ethanol recovery rate ≥ 98%; Utilization of by-products: The soybean hydrolysis residue is converted into β-glucan (yield ≥ 25%) through solid-state fermentation and used in the hyaluronic acid fermentation medium; Carbon footprint optimization: A photovoltaic power supply module is integrated into the system (conversion efficiency ≥ 22%) to reduce the CO2 emissions per batch of production by 63%.

[0022] This solution constructs a new generation of intelligent natural skin care product preparation system through interdisciplinary innovations such as quantum computing, synthetic biology, and nanotechnology.

[0023] In this example, the pure natural freckle-removing and whitening liquid prepared by this method (Group A) and the control group without adding any treatment agents (Group B) are used as research objects. Melanoma cells with high activity and stable genetic characteristics are cultured in an incubator at 37°C and 5% CO2. The pure natural freckle-removing and whitening liquid in Group A is diluted to three different concentrations of 2% (Group A1), 0.2% (Group A2), and 0.02% (Group A3), and are respectively added to the cell culture medium. Three replicate experiments are set for each concentration. After 72 hours of continuous culture, the morphological changes of cells in each group are observed using an optical microscope; the tyrosinase activity in the cell culture supernatant of each group is quantitatively analyzed by enzyme-linked immunosorbent assay, and the results are recorded in Table 1. From the above in vitro cell experiments, it can be seen that the cell morphology in Group A is more regular and the cell gap is reduced, indicating that the pure natural freckle-removing and whitening liquid prepared by this method has a positive effect on the growth state of cells. Compared with Group B, the tyrosinase activity in Group A1 decreased most significantly, reaching a reduction rate of 40%. The tyrosinase activities in Group A2 and Group A3 decreased by 25% and 18% respectively. This shows that the pure natural freckle-removing and whitening liquid prepared by this method can effectively inhibit the activity of tyrosinase within a certain concentration range and can effectively improve the freckle-removing performance.

[0024] Example 2 A preparation method of a pure natural freckle-removing and whitening liquid, characterized in that: the preparation method includes: Step 1, prepare the raw materials for the preparation of the pure natural freckle-removing and whitening liquid and the reaction device. The raw materials for the preparation of the pure natural freckle-removing and whitening liquid include niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, citric acid, glycerol and pure water. The niacinamide, vitamin C, vitamin E and hyaluronic acid are all prepared from natural substances. The ratio of the raw materials is preset and optimized by using a quantum optimization algorithm model. The reaction device uses a microwave high-efficiency extraction instrument to achieve automatic extraction. The quantum optimization algorithm model includes an initialization module, a quantum evolution module, an observation and reading module, and an update and decision module; The initialization module is the starting point of the quantum optimization algorithm model, determines the coding method, improves the parallelism, and provides a basis for subsequent quantum operations; The quantum evolution module realizes the manipulation of the state of quantum bits through the operation of Hadamard gates or phase flip gates, and makes the system evolve in the direction of lower energy and better solutions by designing a suitable Hamiltonian operator; The observation and reading module is the process of converting quantum information into classical information. By repeating experiments multiple times and statistically analyzing the probability distribution of the results, the position of the optimal solution is estimated; The update and decision module updates the solution to the problem according to the observation results and makes a decision on whether to continue iteration; Step 2, extract aspartic acid from soybeans by the protein hydrolysis method, and then sequentially add aspartic acid oxidase, quinolinic acid phosphoribosyltransferase and nicotinic acid mononucleotide enzyme to obtain the niacinamide. The reaction formula for preparing the niacinamide is: In formula (1), is the aspartic acid, wherein nicotinamide is represented as [[nicotinamide]], and H2O represents water molecules; Step 3: Add the pulverized bearberry leaf powder, licorice powder, lavender powder, and ethanol to a microwave high-efficiency extractor. Input the type and state of the sample into the intelligent control system of the microwave high-efficiency extractor. The microwave high-efficiency extractor automatically adjusts the microwave power and irradiation time. Finally, take out the sample, and use high-performance liquid chromatography technology to separately obtain arbutin with a purity of 99%, glycyrrhizic acid with a purity of 99%, tea polyphenols with a purity of 99%, and lavender essential oil with a purity of 99% from the sample; Step 4: Put the squeezed citrus fruit juice into a bioreactor filled with microalgae organisms. Control the temperature of the bioreactor to be 27 - 31°C and the pH to be 4.7 - 5.4. By continuously introducing oxygen with a volume fraction of 21%, the microalgae organisms in the bioreactor convert the fruit juice into dehydroascorbic acid. Then add invertase to reduce the dehydroascorbic acid to the vitamin C. Finally, perform final purification on the vitamin C through microfiltration membrane filtration, ion exchange resin adsorption, and high-performance liquid chromatography technology to obtain vitamin C with a purity of 99%. The equation for the conversion of dehydroascorbic acid to vitamin C is: In formula (2), represents dehydroascorbic acid, and [[vitaminC]] represents vitamin C; Step 5: Use cold pressing technology to process wheat germ to obtain wheat germ oil rich in tocotrienols. Then add the wheat germ oil to a microwave high-efficiency extractor to extract tocotrienols. Then add the enzyme preparation cyclooxygenase, control the reaction temperature to be 37°C and the time to be 12 h, and convert the tocotrienols to vitamin E. The equation for the conversion of tocotrienols to vitamin E is: In formula (3), represents tocotrienols, and [[vitaminE]] represents vitamin E; Step 6: Add Streptococcus strains to a culture medium containing 65% glucose, 34% yeast powder, a culture medium with a concentration of 4 ml / L, a biostimulant made from plant extracts, and trace elements 0.5% zinc, 0.3% manganese, 0.1% sodium, and 0.1% selenium. Control the temperature to be 37°C, the pH to be 7.0, and the oxygen concentration to be 2%. After fermentation for 12 h, collect the fermentation product containing a large amount of [[hyaluronicAcid]], and obtain [[hyaluronicAcid]] with a purity of 99% through continuous centrifugation and filtration systems and ethanol precipitation; Step 7: Mix the niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, glycerol and pure water according to a preset ratio, adjust the pH to 5.5 - 6.5 with the citric acid, add the mixture to the mixing tank of a steady-state melting machine, control the rotation speed at 5000 - 11000 r / min for high-speed shear mixing for 0.5 h, and then adjust the rotation speed to 3000 r / min for fine shear for 2 h until the uniform and delicate natural freckle-removing and whitening liquid is formed. In a specific embodiment, the natural freckle-removing and whitening liquid prepared by this method (Group A) and a control group (Group B) without adding any treatment agent in this embodiment are used as research objects for antioxidant capacity testing. The natural freckle-removing and whitening liquid of Group A is respectively formulated into two different concentrations of 0.5 mg / mL (Group A1) and 1 mg / mL (Group A2). The Group A1 and Group A2 are respectively mixed with 50 mL of DPPH solution, and the mixture is placed in the dark. After 2 h of reaction, the absorbance value at 517 nm is measured using a spectrophotometer. The control group is a mixture containing only DPPH solution without adding the natural freckle-removing and whitening liquid prepared by this method. Calculate the scavenging rate, and the scavenging rate (%) = Recorded in Table 2. Example 3 In the above embodiment, the mass parts of the preparation raw materials include 15 mass parts of the niacinamide, 13 mass parts of the arbutin, 11 mass parts of the glycyrrhizic acid, 9 mass parts of the tea polyphenols, 19 mass parts of the lavender essential oil, 2 mass parts of the vitamin C, 1.3 mass parts of the vitamin E, 5 mass parts of the hyaluronic acid, 0.9 mass parts of the citric acid, 8 mass parts of the glycerol and 100 mass parts of the pure water. As a further technical solution of the present invention, the working method of the quantum evolution module in Step 1 is: by analyzing the state and evolution history of the quantum system in real time, dynamically adjusting the parameters of the quantum gates, determining the "hot spot" area of the search space by monitoring the entropy of the quantum state distribution, and correspondingly enhancing the search accuracy in this area. This adaptive strategy is expressed by the formula: In formula (6), is the quantum gate operation at time step t, and f is the adjustment function obtained according to the system state entropy obtained, Quantum gate operations based on this ensure that the evolution of the quantum system has sufficient randomness for global search and a clear direction for local fine exploration. In the above embodiment, the working method of the protein hydrolysis method in step 2 is as follows: Soak the selected soybeans in a buffer solution with a pH of 7.0. The buffer solution is a citric acid and sodium hydroxide buffer system with a molar ratio of 3:1. Add 10% of the hydrolysis initiation enzyme trypsin and hydrolyze at a constant temperature of 30 °C for 1 h. Then, sequentially add 5% of the aspartate oxidase, 4.5% of the quinolinic acid phosphoribosyltransferase, and 3% of the nicotinic acid mononucleotide enzyme to obtain the nicotinamide.

[0025] In the above embodiment, the working method of the high-performance liquid chromatography technique in step 3 is as follows: Fill the chromatographic column packing with silica with a particle size of 8 microns and a specific surface area of 100,000 m² / g. Control the temperature at 25 °C and the pressure at 101.3 KPa. Flow the solution containing the arbutin through the chromatographic column to specifically absorb the arbutin and elute other components. Subsequently, use an ethanol eluent to elute and collect the arbutin to obtain the arbutin with a purity of 99%.

[0026] In a specific embodiment, this embodiment uses 10 volunteers (A, B, C, D, E, F, G, H, I, and J) as the research objects for a human skin test. Before the test starts, use a professional color difference meter to conduct a preliminary color difference measurement on the inner arm skin of each volunteer and record the original data before applying the pure natural freckle-removing and whitening liquid prepared by this method. Apply the pure natural freckle-removing and whitening liquid to the inner arm of the volunteer twice a day. On the first day after 21 consecutive days of use, use the same color difference meter to test the inner arm skin of the volunteer and record the results in Table 3. The test results are shown in Table 3. It can be seen that the pure natural freckle-removing and whitening liquid prepared by this method has a prominent overall brightening and whitening effect on skin color, and the effect on changing the redness and yellowness of skin color is relatively weak. This shows that this method can effectively improve the brightening and whitening effect of the freckle-removing and whitening liquid and has better performance in practical applications.

[0027] Example 4 In the above embodiments, the microwave high-efficiency extraction instrument includes a microwave generator 1, a microwave transmission system 2, a sample container 3, a solvent system 4, a control system 5, a collection system 6, and a cooling system 7. The microwave generator 1 consists of a magnetron and is located at the center of the entire microwave high-efficiency extraction instrument. The microwave transmission system 2 is connected to the sample container 3 in a through connection by welding. The microwave transmission system 2 is connected to the microwave generator 1 in a through connection by welding. The solvent system 4 is located on the right side of the sample container 3 by welding, adding or circulating a specific solvent into the sample container 3. The control system 5 is welded to the upper end of the microwave generator 1 to control the power of the microwave generator 1 and monitor parameters such as temperature, pressure, and time during the entire extraction process. The collection system 6 is welded to the end of the entire device. The cooling system 7 is located around the sample container 3 by welding. The process of the microwave high-efficiency extraction instrument will be exemplarily described through specific embodiments below. When the present invention is in operation, the working method of the microwave high-efficiency extraction instrument is as follows: Add the raw materials into the sample container 3, select a suitable solvent and add it into the solvent system 4, turn on the microwave generator 1, adjust parameters such as power and time. Under the action of the microwave transmission system 2, the microwave energy uniformly heats the sample to fully release the active ingredients. At the same time, the control system 5 monitors parameters such as temperature and pressure in real time to ensure the stability and accuracy of the experiment. Finally, the sample container 3 is cooled by the cooling system 7, and the extract is collected through the collection system 6 to finally obtain the extract.

[0028] Example 5 In the above embodiments, a method for using a pure natural freckle-removing and whitening liquid, the method for using a pure natural freckle-removing and whitening liquid includes the following steps: Step 1, Clean the skin. After wetting the face with warm water, take a pea-sized mild facial cleanser and gently massage the entire face. Thoroughly remove the oil and dirt on the skin surface and then rinse with clean water; Scan the face with a handheld multispectral skin analyzer with a wavelength range of 400 - 1100 nm to generate a thermal map of skin freckle distribution with a resolution of 0.1 mm² / pixel in real time; Step 2, Based on the convolutional neural network CNN algorithm module, identify freckles, melasma, and post-inflammatory hyperpigmentation, and calculate the proportion of the freckle area with an accuracy of ±1.2%; Synchronize the data to the mobile terminal APP via Bluetooth to generate a personalized usage plan; Pat the skin gently with a towel until it is semi-dry, maintaining a certain degree of moisture to make the active ingredients penetrate better; When the proportion of the freckle area > 15%, activate the targeted penetration mode: Load the whitening liquid onto a microneedle patch with a needle length of 150 μm and a density of 200 needles / cm², and use a programmable microcurrent iontophoresis instrument with a current intensity of 0.3 mA / cm² and a frequency of 10 Hz to deliver the active ingredients directionally; When the area of ​​pigmentation is ≤15%, the photodynamic synergy mode is activated: with an LED phototherapy device with a wavelength of 415nm and an irradiance of 50mW / cm², the vitamin C derivatives in the whitening solution are activated; Step 3: Take a rice-sized amount of the pure natural freckle-removing and whitening liquid and perform a local applicability test behind the ear to ensure safety and no allergic reaction; the whitening liquid adopts a thermosensitive liposome encapsulation technology with a phase transition temperature of 32°C. When the liposome contacts the skin, it ruptures and releases the active substance; The integrated 50μm-thick flexible electronic skin patch has a built-in pH sensor with an accuracy of ±0.1 and a humidity sensor with an accuracy of ±3%RH to monitor the skin microenvironment in real time: When pH>6.0 is detected, the sustained-release system is triggered to extend the release time of arbutin; When the humidity is less than 60%RH, the gradient release mechanism of hyaluronic acid is activated; Step 4: First squeeze out one pump and apply evenly on the whole face, then squeeze out another pump and apply locally on the area with serious spots; massage with the intelligent piezoelectric massage head, which monitors the skin absorption status in real time through impedance analysis with a frequency of 1MHz and a sensitivity of 0.1Ω. The massage time is 5-10 minutes; Step 5: Spray a bioactive protective film after use. The film-forming material is a silk fibroin-dextran composite with a mass ratio of 7:3 and an oxygen permeability of ≥500cm³ / (m²·d). Detailed description of the implementation process: Step 1: Smart skin diagnosis and cleansing 1. Skin pretreatment Wet your face with warm water (32-35°C), take a soybean-sized amount (about 0.5 mL) of amino acid-based mild cleanser (pH 5.5-6.0), and gently massage your face in circular motions for 60 seconds, focusing on the T-zone and jawline.

[0029] After rinsing with clean water (hardness ≤ 100ppm), use a microfiber towel (fiber diameter ≤ 5μm) to absorb the surface moisture, retaining 20-30% of the moisture content of the skin's stratum corneum.

[0030] 2. Multispectral Skin Analysis Start the handheld multispectral skin analyzer, the scanning mode covers the 400-1100nm band (including visible light and near infrared), and the light source intensity is ≤10mW / cm² (in compliance with IEC 62471 photobiological safety standards).

[0031] The skin reflectance spectrum is captured by a CMOS image sensor (resolution 1280×960 pixels), and combined with an RGB-NIR multi-channel image fusion algorithm, a 0.1mm² / pixel color spot distribution heat map is generated (pseudo-color coding: red = high pigment density, blue = low pigment density).

[0032] Step 2: AI Diagnosis and Personalized Solution Execution 1. Intelligent Identification of Skin Spots Convolutional Neural Network (CNN) Model Architecture: Input Layer: 512×512 pixel multi-spectral image Convolutional Layer: 5 layers (kernel size 3×3, ReLU activation) Fully Connected Layer: Output the probability of skin spot type (Softmax function) and the area ratio (Sigmoid function) Example of Diagnostic Result Output: Melasma: Area ratio 18.7% ±1.2% Post-inflammatory Hyperpigmentation (PIH): Area ratio 6.3% ±1.2% 2. Solution Decision-making and Execution Targeted Penetration Mode (skin spot area > 15%): Load the whitening liquid into the soluble microneedle patch (material: hyaluronic acid-polyvinyl alcohol copolymer), the microneedle array density is 200 needles / cm², and the tip loading is 0.15 μL / needle.

[0033] Parameters of the Microcurrent Electroosmosis Promoter: Waveform: Biphasic Square Wave (duty cycle 50%) Electrode Layout: Ring Array (anode surrounds the microneedle area) Electroosmotic Flow Rate: 1.2 μL / (cm²·min) Photodynamic Synergy Mode (skin spot area ≤ 15%): Irradiation Parameters of the LED Phototherapy Instrument: Wavelength 415 nm (half-peak width ±10 nm), spot diameter 5 cm Irradiation Time: 10 minutes (divided into 3 pulse cycles, each cycle is 3 minutes of irradiation + 1 minute of interval) Photoactivation Mechanism: The efficiency of vitamin C derivative (ascorbic acid glucoside) to produce reactive oxygen species (ROS) under blue light excitation is increased by 3 times.

[0034] Step 3: Safety Test and Intelligent Delivery 1. Allergy Test Apply a whitening liquid the size of a rice grain (about 0.1 mL) to the skin behind the ear (thickness 50 - 80 μm) and let it stand for 15 minutes.

[0035] Monitor through a flexible electronic patch: Histamine Release Amount (electrochemical sensor, detection limit 0.1 ng / mL) Change in Erythema Index (a value) (Δa < 1.5 is the safety threshold) 2. Release of Thermosensitive Liposomes Liposome composition: DPPC (dipalmitoyl phosphatidylcholine): DSPE-PEG2000 = 9:1 (mol ratio) Phase transition kinetics: After contacting the skin (temperature ≥ 32°C), the lipid bilayer structure completely disintegrates within 120 seconds Burst release rate of active ingredient: 60% is released in the first 5 minutes, and 40% is released in a sustained release manner subsequently 3. Environment-responsive control pH regulation (when pH > 6.0 is detected): The release system switches to the ion exchange resin controlled release module (carboxymethyl cellulose carrier), and the release half-life of arbutin is extended from 2 hours to 6 hours

[0036] Humidity regulation (when humidity < 60%RH is detected): Hyaluronic acid is released in a gradient manner driven by a micropump: 30% is released in 0 - 2 hours, 50% is released in 2 - 6 hours, and 20% is released in 6 - 12 hours

[0037] Step 4: Precise application and biofeedback massage 1. Zonal application strategy Full face application: Press the metering pump (0.8 mL per pump) to evenly cover the face (dosage 0.05 mL / cm²), avoiding the periorbital area (≥ 5 mm from the eyelid margin)

[0038] Local reinforcement: Press the metering pump again and apply a spot application to the pigmented area (diameter > 2 mm), with double the dosage (0.1 mL / cm²)

[0039] 2. Intelligent massage control Impedance monitoring: The piezoelectric massage head emits a 1 MHz AC signal (voltage ≤ 5 V), and the skin impedance is measured by the four-electrode method (baseline value: dry skin > 200 Ω·cm, wet skin < 80 Ω·cm)

[0040] When the local impedance decrease rate > 30%, it is determined that the component absorption is saturated

[0041] Dynamic parameter adjustment: Initial pressure 0.5 N (frequency 5 Hz), increasing by 0.5 N every 2 minutes to a maximum of 3 N Blood flow monitoring (PPG sensor): If the local blood oxygen saturation (SpO2) increases > 15%, automatically switch to the low-frequency mode (8 Hz) Step 5: Long-term protective film construction 1. Film-forming process Spraying equipment: Airless electrostatic spray gun (atomization particle size 20 - 50 μm), moving uniformly 15 cm away from the skin

[0042] Film formation process: Initial layer: Silk fibroin (molecular weight 70 kDa) forms a dense basement membrane (thickness 5 μm) Outer layer: Dextran (DE value 15 - 20) constructs a porous structure (porosity 40%) 2. Function realization Sustained release system: PLGA microspheres (lactic acid: glycolic acid = 75:25) loaded with SOD mimetic enzyme (manganese - coordinated superoxide dismutase), and the release kinetics at 25°C conforms to the Higuchi model (R² > 0.98).

[0043] Light protection indication: Prussian blue nanoparticles (particle size 50 nm) undergo ligand - field transition under UVA irradiation, and the color changes from colorless to blue (CIE Lab value: Δb > 20).

[0044] Color - change threshold: Ultraviolet intensity > 3 mW / cm² for 5 minutes.

[0045] Verification of technical effects: Transdermal efficiency: Franz diffusion cell test shows that the 24 - hour cumulative penetration amount of arbutin reaches 82.3 μg / cm² (traditional method: 46.7 μg / cm²).

[0046] Sustained action: 12 hours after film formation, the vitamin C concentration on the skin surface remains ≥ 15 μg / cm² (detected by HPLC).

[0047] Safety: Through double - blind tests on 30 people, the irritation score is 0.21 ± 0.05 (0 - 4 - level standard), and the allergy incidence is 0%.

[0048] This implementation process realizes the whole - chain scientific skin care from diagnosis to protection through a technical closed - loop of multi - modal perception - intelligent decision - making - precise execution, meeting the medical - grade precise beauty needs.

[0049] In a specific embodiment, this embodiment takes the pure natural freckle - removing and whitening liquid prepared by this method as the research object for a light stability experiment. The pure natural freckle - removing and whitening liquid is divided into 20 - mL equal - volume samples, marked as group A, group B, and group C. Ultraviolet light, simulated sunlight, and LED light sources are set, and the irradiation intensity of the light source is adjusted to 100 lux. Samples in group A, group B, and group C are respectively exposed to the ultraviolet light source, simulated sunlight, and LED light source for continuous irradiation for 1 h. During the experiment, the changes in parameters such as the appearance, smell, and color of the samples are recorded every 10 min. The color change of the samples is measured using a colorimeter, and the specific values are recorded. The change in the smell of the samples is analyzed and recorded using an electronic nose, and the results are recorded in Table 4. The experimental results are shown in Table 4. It can be seen that the stability of the pure natural freckle-removing and whitening liquid prepared by this method is poor under ultraviolet irradiation and good under LED light source. This indicates that during actual storage and use, long-term exposure to strong ultraviolet rays should be avoided as much as possible to improve the stability and usage effect of the product.

[0050] Although the specific embodiments of the present invention have been described above, those skilled in the art should understand that these specific embodiments are merely illustrative. Without departing from the principles and essence of the present invention, those skilled in the art can make various omissions, substitutions, and changes to the details of the above methods and systems. For example, combining the above method steps so as to perform substantially the same function in a substantially the same way to achieve substantially the same result falls within the scope of the present invention. Therefore, the scope of the present invention is only defined by the appended claims.

Claims

1. A preparation method of a pure natural freckle-removing and whitening liquid, characterized in that: The preparation method includes: Step 1: Prepare the raw materials for the preparation of the natural freckle-removing and whitening liquid and the reaction device. The raw materials for the preparation of the natural freckle-removing and whitening liquid include niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, citric acid, glycerol and pure water. The niacinamide, vitamin C, vitamin E and hyaluronic acid are all prepared from natural substances. The ratio of the raw materials is preset and optimized by using a quantum optimization algorithm model. The reaction device uses a microwave high-efficiency extraction instrument to achieve automatic extraction. The quantum optimization algorithm model includes an initialization module, a quantum evolution module, an observation and reading module, and an update and decision-making module; The initialization module, which is the starting point of the quantum optimization algorithm model, determines the coding method, improves parallelism, and provides a basis for subsequent quantum operations; The quantum evolution module realizes the manipulation of the quantum bit state through the operation of the Hadamard gate or the phase flip gate. By designing a suitable Hamiltonian operator, the system evolves in the direction of a lower energy and better solution; The observation and reading module is the process of converting quantum information into classical information. By repeating experiments multiple times and statistically analyzing the probability distribution of the results, the position of the optimal solution is estimated; The update and decision-making module updates the solution to the problem according to the observation results and makes a decision on whether to continue iteration; Step 2: Aspartic acid is extracted from soybeans by the protein hydrolysis method, and then aspartate oxidase, quinolinate phosphoribosyltransferase, and nicotinic acid mononucleotide enzyme are sequentially added to obtain the said nicotinamide. The reaction formula for preparing the nicotinamide is as follows: In formula (1), is the said aspartic acid, is the said nicotinamide, and H2O is a water molecule; Step 3: Add the pulverized arbutus leaf powder, licorice powder, lavender powder and ethanol to the microwave high-efficiency extraction instrument respectively. Input the type and state of the sample into the intelligent control system of the microwave high-efficiency extraction instrument. The microwave high-efficiency extraction instrument automatically adjusts the microwave power and irradiation time. Finally, take out the sample and obtain the arbutin with a purity of 99%, the glycyrrhizic acid with a purity of 99%, the tea polyphenols with a purity of 99% and the lavender essential oil with a purity of 99% from the sample by using high-performance liquid chromatography technology; Step 4: Put the squeezed citrus fruit juice into a bioreactor filled with microalgae organisms. Control the temperature of the bioreactor at 27 - 31 °C and the pH at 4.7 - 5.

4. By continuously introducing oxygen with a volume fraction of 21%, the microalgae organisms in the bioreactor convert the fruit juice into dehydroascorbic acid. Then, add invertase to reduce the dehydroascorbic acid into the vitamin C. Finally, conduct final purification of the vitamin C through microfiltration membrane filtration, ion exchange resin adsorption, and high-performance liquid chromatography technology to obtain the vitamin C with a purity of 99%. The equation for the conversion of dehydroascorbic acid into the vitamin C is: In formula (2), is the dehydroascorbic acid, is the vitamin C; Step 5: Treat wheat germ with cold pressing technology to obtain wheat germ oil rich in tocotrienols, then add the wheat germ oil to a microwave high-efficiency extractor to extract the tocotrienols, and then add the enzyme preparation cyclooxygenase, control the reaction temperature at 37°C and the time at 12 h to convert the tocotrienols into the vitamin E. The equation for converting tocotrienols into vitamin E is: In formula (3), is the tocotrienol, is the vitamin E; Step 7: Add the streptococcus strain to a culture medium containing 65% glucose, 34% yeast powder, a culture medium with a concentration of 4 ml / L, a biostimulant made from plant extracts and trace elements of 0.5% zinc, 0.3% manganese, 0.1% sodium and 0.1% selenium. Control the temperature at 37 °C, the pH at 7.0 and the oxygen concentration at 2%. After fermentation for 12 h, collect the fermentation product containing a large amount of the hyaluronic acid, and obtain the hyaluronic acid with a purity of 99% through a continuous centrifugation and filtration system and by using ethanol precipitation; Step 8: Mix the niacinamide, arbutin, glycyrrhizic acid, tea polyphenols, lavender essential oil, vitamin C, vitamin E, hyaluronic acid, glycerol and pure water in a preset ratio, adjust the pH to 5.5 - 6.5 with the citric acid, add the mixture to the mixing tank of a steady-state melting machine, control the rotation speed at 5000 - 11000 r / min for high-speed shear mixing for 0.5 h, and then adjust the rotation speed to 3000 r / min for fine shear for 2 h until the uniform and delicate natural freckle-removing and whitening liquid is formed.

2. The preparation method of a pure natural freckle-removing and whitening liquid according to claim 1, wherein: The quantum optimization algorithm model establishes the correlation function between the proportion of the preparation raw materials and the performance indexes of the pure natural freckle-removing and whitening liquid, and the sample set composed of the preparation raw materials is The expression of the correlation function is: In formula (4), F is the performance index of the pure natural freckle-removing and whitening liquid, M and N are weights, is the proportion of the preparation raw materials, i is the ordinal number of the preparation raw materials, a is an element in U, and p is an element in U. Finally, the evaluation criterion is used to evaluate whether the proportion of the preparation raw materials can synthesize the optimal performance index of the pure natural freckle-removing and whitening liquid. The correlation function of the evaluation criterion is: In formula (5), is the evaluation criterion, C is the label set, is the information of the proportion of the preparation raw materials, is the evaluation criterion of the coordinates of the preparation raw materials. When the performance index of the pure natural freckle-removing and whitening liquid is optimal. The implementation process of the quantum optimization algorithm is as follows: First, initialize the proportion of the preparation raw materials and the performance index of the pure natural freckle-removing and whitening liquid, determine an initial quantum state, then construct the Hamiltonian of the system according to the correlation function, then use quantum gate operations to evolve the initial quantum state, measure the evolved quantum state, obtain a set of candidate solutions for the proportion of the preparation raw materials, and finally convert the measurement result into the actual proportion of the preparation raw materials and calculate the corresponding performance index F of the pure natural freckle-removing and whitening liquid.

3. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, characterized in that: The mass parts of the preparation raw materials include 15 mass parts of the nicotinamide, 13 mass parts of the arbutin, 11 mass parts of the glycyrrhizic acid, 9 mass parts of the tea polyphenols, 19 mass parts of the lavender essential oil, 2 mass parts of the vitamin C, 1.3 mass parts of the vitamin E, 5 mass parts of the hyaluronic acid, 0.9 mass parts of the citric acid, 8 mass parts of the glycerol and 100 mass parts of the pure water.

4. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, characterized in that: The working method of the quantum evolution module in the first step is as follows: By analyzing the state and evolution history of the quantum system in real time, dynamically adjust the parameters of the quantum gates, determine the "hot" regions of the search space by monitoring the entropy of the quantum state distribution, and accordingly enhance the search accuracy in this region. This adaptive strategy is expressed by the formula: In formula (6), is the quantum gate operation at time step t, and f is the adjustment function obtained according to the entropy of the system state derived, is the quantum gate operation based on this. In this way, we can ensure that the evolution of the quantum system has sufficient randomness for global search and clear direction for local fine exploration.

5. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, wherein: The working method of the protein hydrolysis method in the second step is as follows: Soak the selected soybeans in a buffer solution with a pH of 7.

0. The buffer solution is a citric acid and sodium hydroxide buffer system with a molar ratio of 3:

1. Add 10% of the hydrolysis starting enzyme trypsin and hydrolyze at a constant temperature of 30 °C for 1 h. Then, sequentially add 5% of the aspartate oxidase, 4.5% of the quinolinic acid phosphoribosyltransferase and 3% of the nicotinic acid mononucleotide enzyme to obtain the nicotinamide.

6. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, characterized in that: The working method of the high performance liquid chromatography technique in the third step is as follows: Fill silica with a particle size of 8 microns and a specific surface area of 100,000 m² / g in the chromatographic column filler. Control the temperature at 25 °C and the pressure at 101.3 KPa. Let the solution containing the arbutin flow through the chromatographic column so that the arbutin is specifically adsorbed and other components are eluted. Subsequently, use an ethanol eluent to elute and collect the arbutin to obtain the arbutin with a purity of 99%.

7. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, characterized in that: The microwave high-efficiency extractor includes a microwave generator (1), a microwave transmission system (2), a sample container (3), a solvent system (4), a control system (5), a collection system (6) and a cooling system (7). The microwave generator (1) consists of a magnetron and is located at the center of the entire microwave high-efficiency extractor. The microwave transmission system (2) is connected to the sample container (3) in a through connection by welding. The microwave transmission system (2) is connected to the microwave generator (1) in a through connection by welding. The solvent system (4) is located on the right side of the sample container (3) by welding, adding or circulating a specific solvent to the sample container (3). The control system (5) is welded to the upper end of the microwave generator (1) to control the power of the microwave generator (1) and monitor parameters such as the temperature, pressure and time of the entire extraction process. The collection system (6) is welded to the end of the entire device. The cooling system (7) is located around the sample container (3) by welding.

8. The preparation method of a natural freckle-removing and whitening liquid according to claim 1, characterized in that: The steady-state fining machine includes an energy input unit (8), a stability control module (9), a fine fusion chamber (10) and an output port (11). The energy input unit (8) is connected to the outside of the entire device by an electric circuit to convert electrical energy into heat energy. The stability control module (9) is connected to the fine fusion chamber (10) in a circuit connection form to monitor the operating state of the sample in the fine fusion chamber (10) and control the stability of the temperature, pressure and energy input in real time. The fine fusion chamber (10) and the output port (11) are connected by a pipeline in a welding manner.

9. A method for using a pure natural freckle-removing and skin-whitening liquid, characterized in that: A method for preparing a pure natural freckle removing and whitening liquid as claimed in any one of claims 1 to 8, wherein the method for using the pure natural freckle removing and whitening liquid comprises the following steps: Step 1. Clean the skin. After moistening the face with warm water, take a soybean-sized amount of mild facial cleanser and gently massage the entire face to thoroughly remove the oil and dirt on the skin surface and then wash it off with clean water; use a handheld multi-spectral skin analyzer with a wavelength range of 400-1100nm to scan the face and generate a skin spot distribution heat map with a resolution of 0.1mm² / pixel in real time; Step 2: Based on the convolutional neural network (CNN) algorithm module, freckles, chloasma, and post-inflammatory pigmentation are identified, and the percentage of pigmentation area is calculated with an accuracy of ±1.2%. The data is synchronized to the mobile terminal APP via Bluetooth to generate a personalized usage plan. Pat the skin with a towel until it is semi-dry, and maintain a certain degree of moisture to allow the active ingredients to penetrate better. When the percentage of pigmentation area is greater than 15%, the targeted penetration mode is activated: the whitening solution is loaded onto a microneedle patch with a needle length of 150μm and a density of 200 needles / cm², and the active ingredients are delivered in a targeted manner through a programmable microcurrent permeation enhancer with a current intensity of 0.3mA / cm² and a frequency of 10Hz. When the area of ​​pigmentation is ≤15%, the photodynamic synergy mode is activated: with an LED phototherapy device with a wavelength of 415nm and an irradiance of 50mW / cm², the vitamin C derivatives in the whitening solution are activated; Step 3: Take a rice-sized amount of the pure natural freckle-removing and whitening liquid and perform a local applicability test behind the ear to ensure safety and no allergic reaction; the whitening liquid adopts a thermosensitive liposome encapsulation technology with a phase transition temperature of 32°C. When the liposome contacts the skin, it ruptures and releases the active substance; The integrated 50μm-thick flexible electronic skin patch has a built-in pH sensor with an accuracy of ±0.1 and a humidity sensor with an accuracy of ±3%RH to monitor the skin microenvironment in real time: When pH>6.0 is detected, the sustained-release system is triggered to extend the release time of arbutin; When the humidity is less than 60%RH, the gradient release mechanism of hyaluronic acid is activated; Step 4: First squeeze out one pump and apply evenly on the whole face, then squeeze out another pump and apply locally on the area with serious spots; massage with the intelligent piezoelectric massage head, which monitors the skin absorption status in real time through impedance analysis with a frequency of 1MHz and a sensitivity of 0.1Ω. The massage time is 5-10 minutes; Step 5: Spray a bioactive protective film after use. The film-forming material is a silk fibroin-dextran complex with a mass ratio of 7:3, and the oxygen permeability is ≥500cm³ / (m²·d).

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