Preparation method and application of zinc-rich hot spring algae extract

Abstract

This invention relates to a method for preparing and applying a zinc-rich hot spring algae extract, belonging to the field of microalgae technology. The invention uses zinc-rich habitat water as the culture medium to cultivate hot spring algae with zinc enrichment; subsequently, a carbonization-based biomimetic extraction technique is used to efficiently extract trace elements such as zinc and strontium from the microalgae; and the entire extraction process does not use organic solvents, thus minimizing environmental impact. The obtained zinc-rich hot spring algae extract can be applied to skincare products for repair, anti-aging, and oil control.

Description

Technical Field

[0001] This invention belongs to the field of microalgae technology and relates to a method for preparing and applying a zinc-rich hot spring algae extract. Background Technology

[0002] In recent years, the application of natural skincare ingredients has received increasing attention from consumers. Hot spring algae refers to algae that live in mineral-rich, high-temperature environments such as hot springs, geothermal springs, and other hot springs. These algae include Scenedesmus, Volvox, Chlorella, Chlamydomonas, Chromococcus, Cryptococcus, Dunaliella, Sheathing Hymena, and Nostoc. While common algae are often rich in polysaccharides and proteins, offering good moisturizing and hydrating effects on the skin, hot spring algae are rich in minerals, trace elements, and antioxidants. These components exhibit good stability at high temperatures and possess effects such as promoting skin barrier repair, anti-inflammation, oil control, anti-oxidation, and anti-aging. Therefore, hot spring algae extracts show promising application prospects in the field of skincare.

[0003] Most existing technologies focus on extracting active substances such as polysaccharides, proteins, and peptides from algae for application in skin care. For example, CN116751313B describes a method for extracting SDEC-18 polysaccharide from Chlorella, and CN118146294A discloses a method for preparing spirulina peptides using a complex protease. Some technologies disclose the enrichment of trace elements in algae, such as CN119081869A achieving selenium-enriched cultivation of Chlamydomonas aeruginosa through high-density fermentation. However, technologies for enriching, cultivating, and extracting zinc from algae are rare. Zinc, as an essential trace element for the human body, has anti-inflammatory, antioxidant, skin barrier repair-promoting, and sebum-regulating effects, and can be widely used in skin care.

[0004] Current technologies do not fully utilize algae for zinc enrichment. When enriching other elements (such as selenium), to avoid the inhibitory effect of selenium on algal growth, it is usually necessary to gradually add selenium to acclimate the algae, a process that is time-consuming. Furthermore, no specific extraction techniques are employed for selenium extraction from algae, and the selenium content obtained from algal extracts obtained using general extraction processes is relatively low. Summary of the Invention

[0005] This invention relates to a method for preparing and applying a zinc-rich hot spring algae extract, belonging to the field of microalgae technology. The invention uses zinc-rich habitat water as the culture medium to cultivate hot spring algae with zinc enrichment; subsequently, a carbonization-based biomimetic extraction technique is used to efficiently extract trace elements such as zinc and strontium from the microalgae; and the entire extraction process does not use organic solvents, thus minimizing environmental impact. The obtained zinc-rich hot spring algae extract can be applied to skincare products for repair, anti-aging, and oil control.

[0006] The objective of this invention can be achieved through the following technical solutions:

[0007] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0008] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22-25℃, light intensity to 1500-2000 lux, pH to 7-7.5, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40g / L.

[0009] (2) Add zinc sulfate to the above culture medium to make the zinc content 2-5 mg / L, adjust the light intensity to 2500-3000 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0010] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at a temperature of 700-800℃ for 2-4 hours. Take out the sample after cooling.

[0011] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 5.0-6.5, stir at 20-30℃ for 4-6 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0012] As a preferred technical solution of the present invention, the hot spring algae in step (1) is one of Chlamydomonas or Chlorella.

[0013] As a preferred technical solution of the present invention, the habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0014] As a preferred technical solution of the present invention, the mass ratio of the carbonized hot spring algae sample and the liquid in step (4) is 1g:20-25mL.

[0015] As a preferred technical solution of the present invention, the liquid in step (4) is a biomimetic mineralizing agent solution, which is composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is one of glycine, glutamic acid and methionine; the amino acid content is 0.2-0.5 mol / L, and the nicotinamide adenine dinucleotide content is 0.004-0.01 mol / L.

[0016] Furthermore, the zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0017] The beneficial effects of this invention are:

[0018] (1) The process of this invention is simple, easy to operate and highly efficient. Hot spring habitat water is added to the zinc-rich culture medium as a water source. Since the habitat water itself contains zinc, hot spring algae can adapt well to the zinc-rich environment and rapidly accumulate zinc during the growth process.

[0019] (2) In the process of extracting active ingredients from hot spring algae, the present invention first uses carbonization reaction to fully destroy the cell structure of algae, and then uses mineralization inducer to simulate biological environment to gently extract active ingredients such as zinc element, so as to obtain zinc-rich hot spring algae extract.

[0020] (3) No organic solvents are used in the entire process of this invention, which has a small environmental burden. The zinc-rich hot spring algae extract obtained can be used in skin care products. Its by-product hot spring algae biochar can be used in the environmental and energy fields after washing and drying, realizing the diversified utilization of microalgae products. Detailed Implementation

[0021] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with embodiments, is provided below.

[0022] In the following examples and comparative examples, the BG11 culture medium was purchased from Qingdao Haibo Biotechnology Co., Ltd.; Chlamydomonas and Chlorella were purchased from Shanghai Guangyu Biotechnology Co., Ltd.

[0023] Example 1

[0024] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0025] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0026] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0027] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 800℃ for 2 hours. Take out the sample after cooling.

[0028] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0029] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0030] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0031] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0032] The feed solution in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0033] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0034] Example 2

[0035] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0036] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 25℃, light intensity to 2000 lux, pH to 7.5, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0037] (2) Add zinc sulfate to the above culture medium to make the zinc content 3 mg / L, adjust the light intensity to 2700 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0038] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 750℃ for 3 hours. Take out the sample after cooling.

[0039] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.5, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0040] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0041] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0042] The mass ratio of the carbonized hot spring algae sample to the liquid in step (4) is 1g:20mL.

[0043] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.5 mol / L, and the nicotinamide adenine dinucleotide content is 0.01 mol / L.

[0044] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0045] Example 3

[0046] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0047] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 23℃, light intensity to 1700 lux, pH to 7.3, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0048] (2) Add zinc sulfate to the above culture medium to make the zinc content 2 mg / L, adjust the light intensity to 3000 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0049] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 700℃ for 4 hours. Take out the sample after cooling.

[0050] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 5.5, stir at 30°C for 4 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0051] The hot spring algae mentioned in step (1) is Chlorella vulgaris.

[0052] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0053] The mass ratio of the carbonized hot spring algae sample to the liquid in step (4) is 1:20mL.

[0054] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glutamic acid; the amino acid content is 0.4 mol / L, and the nicotinamide adenine dinucleotide content is 0.004 mol / L.

[0055] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0056] Comparative Example 1

[0057] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0058] (1) Inoculate hot spring algae into BG11 medium with deionized water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0059] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0060] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 800℃ for 2 hours. Take out the sample after cooling.

[0061] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0062] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0063] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0064] The feed solution in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0065] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0066] Comparative Example 2

[0067] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0068] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0069] (2) Add zinc sulfate to the above culture medium to make the zinc content 7 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0070] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 800℃ for 2 hours. Take out the sample after cooling.

[0071] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0072] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0073] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0074] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0075] The feed solution in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0076] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0077] Comparative Example 3

[0078] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0079] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0080] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0081] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 600℃ for 2 hours. Take out the sample after cooling.

[0082] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0083] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0084] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0085] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0086] The feed solution in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0087] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0088] Comparative Example 4

[0089] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0090] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0091] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0092] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 800℃ for 2 hours. Take out the sample after cooling.

[0093] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0094] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0095] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0096] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0097] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.1 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0098] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0099] Comparative Example 5

[0100] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0101] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0102] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0103] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 800℃ for 2 hours. Take out the sample after cooling.

[0104] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0105] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0106] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0107] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0108] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.002 mol / L.

[0109] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0110] Comparative Example 6

[0111] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0112] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22℃, light intensity to 1500 lux, pH to 7.0, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0113] (2) Add zinc sulfate to the above culture medium to make the zinc content 5 mg / L, adjust the light intensity to 2500 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0114] (3) Collect zinc-rich hot spring algae and dry them. Add them to the material solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0115] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0116] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0117] In step (4), the ratio of the carbonized hot spring algae sample to the liquid is 1g:20mL.

[0118] The feed solution in step (4) is a biomimetic mineralizing agent solution, composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is glycine; the amino acid content is 0.2 mol / L, and the nicotinamide adenine dinucleotide content is 0.006 mol / L.

[0119] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0120] Comparative Example 7

[0121] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0122] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 24℃, light intensity to 1700 lux, pH to 7.2, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0123] (2) Add zinc sulfate to the above culture medium to make the zinc content 3 mg / L, adjust the light intensity to 2800 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0124] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 750℃ for 3 hours. Take out the sample after cooling.

[0125] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0126] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0127] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0128] The mass ratio of the carbonized hot spring algae sample to the liquid in step (4) is 1:22.

[0129] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of amino acids and deionized water; the amino acid is glycine; the amino acid content is 0.307 mol / L.

[0130] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0131] Comparative Example 8

[0132] A method for preparing a zinc-rich hot spring algae extract includes the following steps:

[0133] (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 24℃, light intensity to 1700 lux, pH to 7.2, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40 g / L.

[0134] (2) Add zinc sulfate to the above culture medium to make the zinc content 3 mg / L, adjust the light intensity to 2800 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae.

[0135] (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at 750℃ for 3 hours. Take out the sample after cooling.

[0136] (4) Add the carbonized hot spring algae sample to the feed solution, adjust the pH to 6.0, stir at 25°C for 5 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract.

[0137] The hot spring algae mentioned in step (1) is Chlamydomonas.

[0138] The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

[0139] The mass ratio of the carbonized hot spring algae sample to the liquid in step (4) is 1:22.

[0140] The liquid in step (4) is a biomimetic mineralizing agent solution, composed of nicotinamide adenine dinucleotide and deionized water; the content of nicotinamide adenine dinucleotide is 0.307 mol / L.

[0141] The zinc-rich hot spring algae extract is used in skincare products for repair, anti-aging, and oil control.

[0142] Performance testing:

[0143] Zinc content test: The zinc content in the hot spring algae extracts of Examples 1-3 and Comparative Examples 1-8 was tested according to the method of GB 5009.14-2017.

[0144] Repair performance test: Prepare HaCaT cells in logarithmic growth phase, at a cell density of 6 × 10⁶. 5 Cells were seeded onto culture plates. Incubation was performed at 37°C with 5% CO2 for 24 hours. Cell damage was created by pipetting, and cells were washed three times with PBS. 2 mL of 0.5 wt% deionized water was added, and incubation continued for another 24 hours. Cells from each group were photographed using an inverted microscope, and the average scratch area was calculated using Image Pro Plus software. The cell migration rate for each group was calculated using the following formula:

[0145] Cell migration rate = (initial scratch area - final scratch area) / initial scratch area × 100%.

[0146] Anti-aging performance test: An in vitro experiment was conducted to promote the production of type I collagen. 2 ml of human skin fibroblasts cultured to the logarithmic growth phase were used at a concentration of 3 × 10⁻⁶ cells / ml. 5 The collagen was implanted at a concentration of 1 / ml into a culture plate with a coverslip. The coverslip was treated with poly-L-lysine before culture. After culturing in DMEM medium containing 10% FBS for 24 hours, the sample solutions of Examples 1-3 and Comparative Examples 1-8 were added at a mass percentage concentration of 0.1%, and the control group was added with an equal volume of serum. After culturing for 7 days, the production of type I collagen was detected and the growth rate was calculated.

[0147] Oil control effect test: 55 volunteers with oily skin were randomly divided into 11 groups. They used 0.5wt% sample aqueous solutions prepared in Examples 1-3 and Comparative Examples 1-8 to replace their usual lotion. They used the solutions twice a day, morning and evening, for 28 consecutive days. After that, the average facial sebum content of the volunteers was measured using a Sebumeter SM 815 sebum analyzer.

[0148]

[0149]

[0150] The test results show that the present invention obtains a zinc-rich hot spring algae extract by using zinc-containing habitat water as the culture medium, followed by carbonization and biomimetic extraction. The HaCaT cell migration rate of the hot spring algae extracts obtained in Examples 1-3 was significantly higher than that of the extracts obtained in Comparative Examples 1-8, indicating that the hot spring algae extracts of the present invention have excellent repair effects; the promoting effect on collagen expression is much higher than that in Comparative Examples 1-8, indicating that the hot spring algae extracts prepared by the method of the present invention have better anti-aging effects; and it can effectively reduce the sebum content of oily skin.

[0151] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any indirect modifications, equivalent changes, and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A method for preparing a zinc-rich hot spring algae extract, characterized in that: Includes the following steps: (1) Inoculate hot spring algae into BG11 medium with habitat water as the water source, adjust the temperature to 22-25℃, light intensity to 1500-2000 lux, pH to 7-7.5, and carry out aeration culture. The enrichment culture is ended when the dry weight of algal cells reaches 40g / L. (2) Add zinc sulfate to the above culture medium to make the zinc content 2-5 mg / L, adjust the light intensity to 2500-3000 lux, continue to culture and take samples every day until the dry weight of algal cells no longer increases, then stop the culture to obtain zinc-rich hot spring algae. (3) Collect zinc-rich hot spring algae and dry them. Add them to a muffle furnace and carbonize them at a temperature of 700-800℃ for 2-4 hours. Take out the sample after cooling. (4) Add the carbonized hot spring algae sample to the material solution, adjust the pH to 5.0-6.5, stir at 20-30℃ for 4-6 hours, centrifuge to obtain the supernatant, filter through a microfiltration membrane and concentrate under reduced pressure to the same weight as the carbonized hot spring algae sample, which is the zinc-rich hot spring algae extract. The mass ratio of the carbonized hot spring algae sample to the liquid in step (4) is 1g:20-25mL; The liquid in step (4) is a biomimetic mineralizing agent solution, which is composed of amino acids, nicotinamide adenine dinucleotide and deionized water; the amino acid is one of glycine, glutamic acid and methionine; the amino acid content is 0.2-0.5 mol / L and the nicotinamide adenine dinucleotide content is 0.004-0.01 mol / L.

2. The method for preparing a zinc-rich hot spring algae extract according to claim 1, characterized in that: The hot spring algae mentioned in step (1) is one of Chlamydomonas or Chlorella.

3. The method for preparing a zinc-rich hot spring algae extract according to claim 1, characterized in that: The habitat water in step (1) contains 0.008 mg / L zinc, 0.487 mg / L silicon, <0.05 mg / L strontium, 0.011 mg / L iron, and 0.0012 mg / L selenium, with the remainder being water and unavoidable impurity elements.

4. The application of a zinc-rich hot spring algae extract prepared by the preparation method according to any one of claims 1-3, characterized in that: It is used in the preparation of skin care products for repair, anti-aging, and oil control.

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