Male anti-dandruff composition applicable to sensitive skin and application

Abstract

The invention discloses a man anti-dandruff composition suitable for sensitive skin and application, and relates to the technical field of wash supplies. The invention provides an anti-dandruff composition. The anti-dandruff composition is prepared from the following components in parts by weight: 0.5-5 parts of a spruce Norway leaf extract, 0.1-2 parts of a hyperforin perforatum extract, 0.1-2 parts of a radix salviae miltiorrhizae extract and 0.5-5 parts of an olive leaf extract. The salvia miltiorrhiza extract is a mixture of a salvia miltiorrhiza root extract and a salvia miltiorrhiza leaf extract. The composition prepared by the invention can be used for repairing the steady state of the scalp from three aspects of fungus proliferation inhibition, sebum secretion regulation and control and barrier function repair, so that better effects of controlling grease and reducing scurf are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of toiletries, in particular to an anti-dandruff composition suitable for men with sensitive skin and its application. Background Art

[0002] In the field of men's personal care, dandruff caused by imbalance of scalp microecology has become a core pain point that affects consumer experience. Currently available men's anti-dandruff shampoos generally have the following technical defects: (1) The anti-dandruff active ingredient has a single target, mainly relying on antifungal agents such as zinc pyrithione and ketoconazole, which only inhibit Malassezia and fail to effectively intervene in the synergistic pathogenic links such as sebum secretion and barrier repair; (2) The proportion of chemical additives in the formula system is too high, causing the pH value of the product to deviate from the normal range of the scalp, and long-term use can easily damage the lipid structure of the stratum corneum; (3) There is a lack of a scalp microecological balance regulation mechanism. Existing technologies mostly use broad-spectrum bactericidal methods, which significantly reduce the abundance of symbiotic bacteria while inhibiting Malassezia, but aggravate the imbalance of the microbial community.

[0003] From the perspective of pathological mechanism, the formation of dandruff is the result of the synergistic effect of multiple pathogenic factors: First, in terms of microecological imbalance, Malassezia secretes lipase to hydrolyze triglycerides in sebum into pro-inflammatory unsaturated fatty acids, which can penetrate into the stratum corneum of the scalp, causing scalp itching, and further leading to excessive keratinization of scalp keratinocytes, accelerating the production of dandruff. Secondly, the androgen sensitivity unique to men leads to a high secretion state of sebaceous glands, which directly stimulates an increase in the rate of sebum secretion. Furthermore, the disorder of the scalp barrier function forms a vicious circle. When the expression of epidermal permeability barrier-related proteins (such as filaggrin and loricrin) is downregulated, the transepidermal water loss rate (TEWL) increases and the barrier is damaged.

[0004] Therefore, developing an anti-dandruff composition that conforms to the physiological characteristics of the scalp and can maintain the balance of the microecology has become a technical problem that needs to be solved urgently in this field. Summary of the invention

[0005] Based on this, the purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a men's anti-dandruff composition and application suitable for sensitive skin with a triple action mechanism of inhibiting fungal proliferation, regulating sebum secretion, and repairing barrier function.

[0006] To achieve the above-mentioned purpose, the technical solution adopted by the present invention is: an anti-dandruff composition, comprising the following components in parts by weight: 0.5-5 parts of Norway spruce leaf extract, 0.1-2 parts of Hypericum perforatum extract, 0.1-2 parts of Salvia miltiorrhiza extract, and 0.5-5 parts of olive leaf extract; the Salvia miltiorrhiza extract is a mixture of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract.

[0007] The extract of Picea abies L. Karst. leaves is rich in polysaccharides, flavonoids and polyphenolic components, which can inhibit the growth of fungi and have a certain anti-inflammatory effect. At the same time, the extract of Picea abies L. Karst. leaves can inhibit the secretion of lipids by sebaceous gland cells and weaken the influence of excessive sebum secretion on dandruff production. The extract of Hypericum perforatum L. contains components such as flavonoids, flavonols and flavanones. These compounds can interfere with the synthesis of the cell membrane of Malassezia, and destroy the structure of the fungal cell membrane by reducing the production of ergosterol, thereby inhibiting the growth of Malassezia. At the same time, the extract of Hypericum perforatum L. can affect the proliferation and differentiation of keratinocytes, thereby enhancing the skin barrier function. The components such as tanshinone and salvianolic acid in the extract of Salvia miltiorrhiza Bunge enhance the anti-inflammatory effect of the composition. At the same time, it can inhibit the activity of 5α-reductase and reduce the excessive sebum secretion induced by DHT. The extract of Olea europaea L. leaves can destroy the integrity of the fungal cell membrane and inhibit the growth of hyphae. And it can regulate the differentiation of sebaceous gland cells and reduce the expression of lipid synthesis-related genes. At the same time, oleuropein in the extract of Olea europaea L. leaves can up-regulate the expression of filaggrin and enhance the water retention ability of the stratum corneum.

[0008] In the actual experimental process, the inventors found that the extracts of Picea abies L. Karst. leaves, Hypericum perforatum L., Salvia miltiorrhiza Bunge and Olea europaea L. leaves are mutually synergistic. The composition prepared can repair the scalp homeostasis from three aspects: inhibiting fungal proliferation, regulating sebum secretion and repairing barrier function, achieving a better effect of controlling oil and reducing dandruff. And in the actual experimental process, it was found that the product prepared with the composition of the present invention can better maintain the microecological balance, and the dandruff problem will not recur after the product is discontinued.

[0009] Preferably, the anti-dandruff composition comprises the following components in parts by weight: 1-3 parts of extract of Picea abies L. Karst. leaves, 0.5-1 part of extract of Hypericum perforatum L., 0.5-1 part of extract of Salvia miltiorrhiza Bunge, and 1-2 parts of extract of Olea europaea L. leaves.

[0010] Preferably, based on the total weight of the anti-dandruff composition, the weight percentage content of the extract of Salvia miltiorrhiza Bunge is 15-25%.

[0011] The inventors found in the actual research that the parts by weight of the components in the anti-dandruff composition provided by the present invention will affect the performance of the product. When the parts by weight of the components are further selected within the above ranges, the product has better effects of inhibiting fungal proliferation, regulating sebum secretion and repairing barrier function.

[0012] Preferably, the weight ratio of the extract of Salvia miltiorrhiza Bunge root to the extract of Salvia miltiorrhiza Bunge leaves is 1:(1-3).

[0013] The polyphenolic components in the extract of Salvia miltiorrhiza Bunge root can inhibit the enzyme in Malassezia that catalyzes the synthesis of CO2 to HCO3 -The activity of the key enzyme β - carbonic anhydrase is inhibited, thereby suppressing the growth of Malassezia; in addition, the root extract of Salvia miltiorrhiza also has the effect of inhibiting the excessive differentiation of sebaceous gland cells; the flavonoids and phenolic acids in the leaf extract of Salvia miltiorrhiza have good anti - inflammatory and antioxidant effects. The inventor found in the actual experiment that when the Salvia miltiorrhiza extract is a mixture of the root extract and the leaf extract of Salvia miltiorrhiza, the effect of controlling sebum and reducing dandruff is better. The inventor found in the actual research that when the weight ratio of the root extract to the leaf extract of Salvia miltiorrhiza in the Salvia miltiorrhiza extract is within the above range, the effect of controlling sebum and reducing dandruff is better.

[0014] Preferably, the root extract and leaf extract of Salvia miltiorrhiza can be commercially available products or can be prepared by oneself.

[0015] Preferably, the preparation method of the root extract of Salvia miltiorrhiza includes the following steps:

[0016] S1. Add the dried, crushed and sieved root of Salvia miltiorrhiza to deionized water, adjust the pH to 3 - 4, then add a composite enzyme. The composite enzyme is a mixture of cellulase, pectinase and acid protease. After enzymatic hydrolysis, collect the enzymatic hydrolysis product;

[0017] S2. Add an organic solvent to the enzymatic hydrolysis product for ultrasonic extraction, filter, collect the filtrate, and dry the filtrate by rotary evaporation to obtain the root extract of Salvia miltiorrhiza.

[0018] Preferably, the weight ratio of the composite enzyme to the root of Salvia miltiorrhiza is 1:(2 - 4). In the composite enzyme, the weight ratio of cellulase, pectinase and acid protease is cellulase:pectinase:acid protease = (1 - 3):(0.5 - 2):(0.1 - 0.5);

[0019] And / or, the organic solvent is an ethanol solution with a volume fraction of 60 - 70%, and the weight ratio of the enzymatic hydrolysis product to the ethanol solution is 1:(20 - 40);

[0020] And / or, the mesh number of the sieving is 800 - 1000 meshes, and the weight ratio of the root of Salvia miltiorrhiza to deionized water is 1:(10 - 20);

[0021] And / or, the temperature of the enzymatic hydrolysis is 40 - 45°C, and the time of the enzymatic hydrolysis is 20 - 30 min; after the enzymatic hydrolysis, centrifuge to collect the enzymatic hydrolysis product, and the rotation speed of the centrifuge is 3000 - 5000 r / min;

[0022] And / or, the temperature of the ultrasonic extraction is 50 - 60°C, the time of the ultrasonic extraction is 15 - 30 min, and the ultrasonic power is 60 - 75 w;

[0023] And / or, the temperature of the rotary evaporation is 40 - 50°C;

[0024] And / or, the drying condition is vacuum drying at 50 - 60°C for 12 - 48 h.

[0025] And / or, the enzyme activity of the cellulase is 20 - 50 U / mg, the enzyme activity of the pectinase is 20 - 50 U / mg, and the enzyme activity of the acid protease is 30 - 80 U / mg.

[0026] Preferably, the preparation method of the Salvia miltiorrhiza leaf extract comprises the following steps:

[0027] (1) Dry and crush the Salvia miltiorrhiza leaves to obtain Salvia miltiorrhiza leaf powder; the mesh number of the Salvia miltiorrhiza leaf powder is 40 - 50 mesh;

[0028] (2) Extract the Salvia miltiorrhiza leaf powder with an ethanol solution in an amount of 8 - 10 times the volume, collect the filtrate, dry it after rotary evaporation to obtain the Salvia miltiorrhiza leaf extract.

[0029] Preferably, the volume fraction of the ethanol solution is 60 - 70%;

[0030] And / or, the extraction is ultrasonic extraction, the temperature of the ultrasonic extraction is 55 - 65°C, the time of the ultrasonic extraction is 1 - 2 h, and the power of the ultrasonic extraction is 60 - 70 W;

[0031] And / or, the temperature of the rotary evaporation is 40 - 50°C;

[0032] And / or, the drying condition is vacuum drying at 50 - 60°C for 12 - 48 h.

[0033] The inventor found in actual research that when the Salvia miltiorrhiza root extract and the Salvia miltiorrhiza leaf extract obtained by the above preparation method are used in combination, when the prepared anti-dandruff composition is applied to the final hair care and washing products, the effects of inhibiting fungal proliferation, regulating sebum secretion, and repairing the barrier function can be better achieved.

[0034] In addition, the present invention provides the application of the anti-dandruff composition in the preparation of hair care and washing products.

[0035] Furthermore, the present invention provides a hair care and washing product, and the hair care and washing product comprises the anti-dandruff composition. The hair care and washing product includes any one of shampoo and conditioner.

[0036] In addition, the present invention provides a shampoo, and the shampoo comprises the following components in mass percentage: 1 - 8% of the anti-dandruff composition of the present invention, 0.7 - 1.5% of a thickener, 15 - 20% of a surfactant, 0.7 - 1% of a preservative, 0.1 - 0.2% of a chelating agent, 0.1 - 0.5% of a pH regulator, and the balance is water.

[0037] Preferably, the thickener includes at least one of sodium chloride, potassium chloride, and hydroxypropyl methylcellulose.

[0038] Preferably, the surfactant includes at least one of polyquaternium-10, cocamidopropyl betaine, potassium coco-hydrolyzed oat protein, sodium methyl cocoyl taurate, cocoglucoside, sodium C14-16 olefin sulfonate, polyquaternium-7, and octanoyl glycine.

[0039] Preferably, the preservative includes at least two of sodium benzoate, phenoxyethanol, and ethylhexylglycerin. Specifically, the mass percentage content of sodium benzoate < 0.5%, and the mass percentage content of phenoxyethanol < 1%.

[0040] Preferably, the chelating agent is disodium EDTA.

[0041] Preferably, the pH regulator is citric acid.

[0042] Compared with the prior art, the beneficial effects of the present invention are as follows: The inventors found in the actual experiment that the extracts of Picea abies leaves, Hypericum perforatum extracts, Salvia miltiorrhiza extracts, and Olea europaea leaf extracts synergistically interact with each other. The prepared composition can repair the scalp homeostasis from three aspects: fungal proliferation inhibition, sebum secretion regulation, and barrier function repair, achieving better control of oil and reducing dandruff. And it was found in the actual experiment that the product prepared with the composition of the present invention can better maintain the microecological balance, and the dandruff problem will not recur after the product is discontinued. Detailed Embodiments

[0043] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. The purpose is to understand the content of the present invention in detail, rather than to limit the present invention. All other embodiments obtained by those of ordinary skill in the art without creative efforts fall within the protection scope of the present invention. The experimental reagents and instruments designed in the embodiments and comparative examples of the present invention are all common ordinary reagents and instruments unless otherwise specified, and can be obtained from commercial channels. In the embodiments and comparative examples, the experimental methods used are all conventional methods unless otherwise specified; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0044] Now, further description will be made on the raw materials used in the present invention, but it is not limited to the following raw materials:

[0045] Extract of Picea abies leaves: Extract of Picea abies leaves, purchased from Norture Company (Norture Lights cell Science Inc.) in Canada;

[0046] Extract of Hypericum perforatum: Purchased from Lena Biotech;

[0047] Olive leaf extract: Purchased from Draco;

[0048] Tremella extract: Purchased from Jiayu Health Technology (Zhejiang) Co., Ltd.;

[0049] Forsythia suspensa extract: Purchased from Shaanxi Guanyiyuan Biotechnology Co., Ltd.;

[0050] Olive fruit extract: Purchased from Fufeng Ciyuan Biotechnology Co., Ltd.

[0051] Cellulase: Enzyme activity 30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.;

[0052] Pectinase: Enzyme activity 30 U / mg, purchased from Hefei Bomei Biotechnology Co., Ltd.;

[0053] Acid protease: Enzyme activity 50 U / mg, purchased from Shanghai Yuanye Biotechnology Co., Ltd.

[0054] Salvia miltiorrhiza root extract A: Prepared in-house, the preparation method is as follows:

[0055] S1. Add the dried and pulverized Salvia miltiorrhiza root that has passed through a 1000-mesh sieve to deionized water. The weight ratio of the Salvia miltiorrhiza root to deionized water is 1:20. After adjusting the pH to 4, add a complex enzyme. The weight ratio of the complex enzyme to the Salvia miltiorrhiza root is 1:3. The complex enzyme is a mixture of cellulase, pectinase, and acid protease, and cellulase:pectinase:acid protease = 2:1:0.3. After enzymatic hydrolysis, centrifuge to collect the enzymatic hydrolysis product; the temperature of the enzymatic hydrolysis is 45°C, the time of the enzymatic hydrolysis is 30 min, and the centrifugation speed is 4000 r / min;

[0056] S2. Add an ethanol solution with a volume fraction of 70% to the enzymatic hydrolysis product for ultrasonic extraction, filter, collect the filtrate, rotary evaporate at 40°C, and then vacuum dry at 50°C for 48 h to obtain the Salvia miltiorrhiza root extract; the weight ratio of the enzymatic hydrolysis product to the ethanol solution is 1:30, the temperature of the ultrasonic extraction is 60°C, the time of the ultrasonic extraction is 20 min, and the ultrasonic power is 70 w.

[0057] Salvia miltiorrhiza root extract B: Prepared in-house, the difference from the preparation method of Salvia miltiorrhiza root extract A is only that: in S1, the ratio of the complex enzyme is different, and cellulase:pectinase:acid protease = 1:2:0.5.

[0058] Salvia miltiorrhiza root extract C: Prepared in-house, the difference from the preparation method of Salvia miltiorrhiza root extract A is only that: in S1, the ratio of the complex enzyme is different, and cellulase:pectinase:acid protease = 0.5:3:0.5.

[0059] Danshen Root Extract D: Self-made. The only difference in its preparation method from Danshen Root Extract A is that in step S1, the types and ratios of the complex enzymes are different. The complex enzyme includes a mixture of cellulase and pectinase, and the ratio of cellulase to pectinase is 2:1.

[0060] Danshen Root Extract E: Self-made. The only difference in its preparation method from Danshen Root Extract A is that in step S2, the volume fraction of the ethanol solution is different. An ethanol solution with a volume fraction of 50% is added to the enzymolysis product for ultrasonic extraction.

[0061] Danshen Leaf Extract A: Self-made. The preparation method is as follows:

[0062] (1) Dry and crush Danshen leaves to obtain Danshen leaf powder; the mesh number of the Danshen leaf powder is 50 mesh.

[0063] (2) Extract the Danshen leaf powder with 10 times the volume of an ethanol solution with a volume fraction of 70%. Collect the filtrate, perform rotary evaporation at 50°C, and then vacuum dry at 50°C for 48 h to obtain the Danshen leaf extract. The extraction is ultrasonic extraction, the temperature of ultrasonic extraction is 65°C, the time of ultrasonic extraction is 1 h, and the power of ultrasonic extraction is 70 W.

[0064] Danshen Leaf Extract B: Self-made. The only difference in its preparation method from Danshen Leaf Extract A is that the volume fraction of the ethanol solution is different. The Danshen leaf powder is extracted with an ethanol solution with a volume fraction of 60%.

[0065] Danshen Leaf Extract C: Self-made. The only difference in its preparation method from Danshen Leaf Extract A is that the volume fraction of the ethanol solution is different. The Danshen leaf powder is extracted with an ethanol solution with a volume fraction of 80%.

[0066] Danshen Leaf Extract D: Self-made. The only difference in its preparation method from Danshen Leaf Extract A is that the volume fraction of the ethanol solution is different. The Danshen leaf powder is extracted with an ethanol solution with a volume fraction of 50%.

[0067] Table 1 shows the weight ratio relationship between Danshen root extract and Danshen leaf extract in Danshen extract. Among them, compared with Danshen extract 1, only the types of Danshen root extract are different in Danshen extracts 2 - 5, and only the types of Danshen leaf extract are different in Danshen extracts 6 - 8.

[0068] Table 1

[0069]

[0070]

[0071] Danshen Extract 9

[0072] Compared with Salvia miltiorrhiza extract 1, the difference lies in the different weight ratios of Salvia miltiorrhiza root extract A and Salvia miltiorrhiza leaf extract A. Specifically, the weight ratio of Salvia miltiorrhiza root extract to Salvia miltiorrhiza leaf extract is 1:1.

[0073] Salvia miltiorrhiza extract 10

[0074] Compared with Salvia miltiorrhiza extract 1, the difference lies in the different weight ratios of Salvia miltiorrhiza root extract A and Salvia miltiorrhiza leaf extract A. Specifically, the weight ratio of Salvia miltiorrhiza root extract to Salvia miltiorrhiza leaf extract is 1:3.

[0075] Salvia miltiorrhiza extract 11

[0076] Compared with Salvia miltiorrhiza extract 1, the difference lies in the different weight ratios of Salvia miltiorrhiza root extract A and Salvia miltiorrhiza leaf extract A. Specifically, the weight ratio of Salvia miltiorrhiza root extract to Salvia miltiorrhiza leaf extract is 1:5.

[0077] Salvia miltiorrhiza extract 12

[0078] Compared with Salvia miltiorrhiza extract 1, the difference lies in the different weight ratios of Salvia miltiorrhiza root extract A and Salvia miltiorrhiza leaf extract A. Specifically, the weight ratio of Salvia miltiorrhiza root extract to Salvia miltiorrhiza leaf extract is 2:1.

[0079] Salvia miltiorrhiza extract 13

[0080] Compared with Salvia miltiorrhiza extract 1, the difference is that it only contains Salvia miltiorrhiza root extract A.

[0081] Salvia miltiorrhiza extract 14

[0082] Compared with Salvia miltiorrhiza extract 1, the difference is that it only contains Salvia miltiorrhiza leaf extract A.

[0083] Examples and comparative examples

[0084] Examples and comparative examples of the present invention provide an anti-dandruff composition, and the selection of components and parts by weight of the composition is shown in Table 2-3; wherein, W represents the weight percentage content of the Salvia miltiorrhiza extract based on the total weight of the anti-dandruff composition; among them, only the types of Salvia miltiorrhiza extracts are different in Examples 1-12, and when comparing Example 1 with Examples 13-16, the selection of parts by weight of each component in the composition is different.

[0085] The preparation method of the composition includes the following steps: mixing each component evenly to obtain the anti-dandruff composition.

[0086] Table 2

[0087]

[0088] Example 2

[0089] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 2 is used to replace Salvia miltiorrhiza extract 1.

[0090] Example 3

[0091] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 3 is used to replace Salvia miltiorrhiza extract 1.

[0092] Example 4

[0093] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 4 is used to replace Salvia miltiorrhiza extract 1.

[0094] Example 5

[0095] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 5 is used to replace Salvia miltiorrhiza extract 1.

[0096] Example 6

[0097] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 6 is used to replace Salvia miltiorrhiza extract 1.

[0098] Example 7

[0099] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 7 is used to replace Salvia miltiorrhiza extract 1.

[0100] Example 8

[0101] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 8 is used to replace Salvia miltiorrhiza extract 1.

[0102] Example 9

[0103] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 9 is used to replace Salvia miltiorrhiza extract 1.

[0104] Example 10

[0105] An embodiment of the present invention provides an anti-dandruff composition. The difference between this anti-dandruff composition and that of Example 1 is only that: Salvia miltiorrhiza extract 10 is used to replace Salvia miltiorrhiza extract 1.

[0106] Example 11

[0107] The embodiment of the present invention provides an anti-dandruff composition. Compared with the embodiment 1, the anti-dandruff composition is different from the embodiment 1 in that the salvia miltiorrhiza extract 1 is replaced by the salvia miltiorrhiza extract 11.

[0108] Example 12

[0109] The embodiment of the present invention provides an anti-dandruff composition. Compared with the embodiment 1, the anti-dandruff composition is different from the embodiment 1 in that the salvia miltiorrhiza extract 1 is replaced by the salvia miltiorrhiza extract 12.

[0110] Table 3

[0111]

[0112]

[0113] Application Examples

[0114] The application examples and comparative application examples of the present invention provide a shampoo, the components (mass percentage) of the shampoo are shown in Table 4; wherein the anti-dandruff compositions used in application examples 1-16 are respectively the anti-dandruff compositions prepared in embodiments 1-16, for example, the anti-dandruff composition in embodiment 1 is used in application example 1, the anti-dandruff composition in embodiment 2 is used in application example 2, and so on; the anti-dandruff compositions used in comparative application examples 1-12 are respectively the anti-dandruff compositions prepared in comparative examples 1-12.

[0115] Table 4

[0116]

[0117] The preparation method of the shampoo provided in Application Example 1 comprises the following steps:

[0118] (1) Add polyquaternium-10 to deionized water and stir to disperse, and then add other materials of phase A after uniform dispersion, heat to 85° C., stir to dissolve uniformly, and then keep warm for standby use to obtain prefabricated phase A;

[0119] (2) Add all ingredients of phase B at 85°C, stir thoroughly to dissolve until transparent, and keep warm to remove foam;

[0120] (3) Cool down to below 45°C, add the raw materials of phase C one by one, and stir evenly;

[0121] (4) Continue to add the raw materials of phase D (previously dissolved in water), mix well, and obtain shampoo.

[0122] The preparation methods of the shampoos provided in the other application examples, comparative application examples, and blank application examples are consistent with that in application example 1, and no relevant components are added if they are not present.

[0123] Application Example 17

[0124] The application example of the present invention provides a shampoo, and the difference between this shampoo and the one in Application Example 1 is that the mass percentage of the anti-dandruff composition is 2%.

[0125] Blank application example

[0126] The blank application example of the present invention provides a shampoo, and the difference between this shampoo and the one in Application Example 1 is that no anti-dandruff composition is added.

[0127] Performance test - 1

[0128] The effect example of the present invention explores the effects of the anti-dandruff compositions prepared in Examples 1 - 16 and Comparative Examples 1 - 12, including the following aspects:

[0129] 1. Anti-inflammatory ability

[0130] The test for anti-inflammatory ability includes the following steps:

[0131] S1. Plating: Take RAW264.7 cells in the logarithmic growth phase and inoculate them into a 24-well plate with a cell density of 2×10 5 cells / mL, 0.5 mL per well. After plating, make marks on the upper cover of the cell plate and place it in a carbon dioxide incubator for incubation for 24 h;

[0132] S2. Sample treatment: After 24 h of cell incubation, aspirate the supernatant, and add the following samples respectively: 0.5 mL: ① Blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ Positive control group: 0.001% dexamethasone (cell culture medium containing 0.001% dexamethasone) + LPS (1 μg / mL); ④ Experimental group: composition (cell culture medium containing 0.5% of the composition of the example or comparative example) + LPS (1 μg / mL);

[0133] S3. Detection of inflammatory factors in cell supernatant: After 24 h of sample treatment of cells, use a pipette to aspirate the supernatant into a centrifuge tube, centrifuge at 1000 r / min for 5 min, discard the precipitate and aspirate the supernatant, and use the Xinbosheng TNF-α inflammatory factor detection kit to detect the change amount of inflammatory factors in the supernatant;

[0134] Calculate the inhibition rate of TNF-α. TNF-α inhibition rate % = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group × 100%; among them, the higher the TNF-α inhibition rate %, the stronger the anti-inflammatory ability;

[0135] The results obtained are shown in Table 5.

[0136] 2. Inhibitory ability against 5α-reductase

[0137] The test for the inhibitory ability against 5α-reductase includes the following steps:

[0138] S1, sample tube: Add 1 mL of sample solution (2% aqueous solution of the composition of the example or comparative example), 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, gently shake well, pipette 200 μL into a 96-well microplate, make three parallels for each sample, put it into a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A 样品0 . After incubating at 37 °C for 20 minutes, put it into a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A 样品20 ; For the positive control, use 2.5 mmol / L finasteride to replace the sample solution;

[0139] S2, enzyme tube: Add 1 mL of PBS solution, 1 mL of enzyme solution, 1 mL of NADPH solution, and 1 mL of testosterone solution into a test tube, gently shake well, pipette 200 μL into a 96-well microplate, make three parallels for each sample, put it into a microplate reader for detection, and measure the absorbance at 340 nm, which is the first measured value A 酶0 . After incubating the sample at 37 °C for 20 minutes, put it into a microplate reader for detection, and measure the absorbance at 340 nm, which is the second measured value A 酶20 . Calculate the inhibition rate of the enzyme according to the following formula;

[0140]

[0141] Among them, the higher the 5α-reductase inhibition rate %, the stronger the oil control ability;

[0142] The results obtained are shown in Table 5.

[0143] 3. Inhibitory ability against Malassezia

[0144] The test for the inhibitory ability against Malassezia includes the following steps:

[0145] The turbidimetric method was used to test the inhibition rate of the composition against Malassezia. Specifically: After culturing Malassezia (ATCC 44344) on a modified Dixon slant medium at 30 °C for 72 h, pick a loop of bacterial strain and streak it on a modified Dixon agar plate. After culturing at 30 °C for 72 h, inoculate it into 50 mL of liquid modified Dixon and shake culture at 30 °C and 130 rpm for 72 h to obtain a bacterial suspension. Use plate counting to obtain its concentration of about 5×10 8 CFU / mL. Add 100 μL of YPD liquid medium and 50 μL of bacterial suspension into a 96-well plate, and mix well. Then add 50 μL of 1% aqueous solution of the composition. Use the one containing only the bacterial suspension as the growth control and the one containing only the medium as the sterile control. After culturing the 96-well plate at 30 °C for 48 h, measure the absorbance at 600 nm. Calculate the inhibition rate according to the following formula.

[0146]

[0147] The obtained results are shown in Table 5 as follows.

[0148] Table 5

[0149]

[0150]

[0151] As can be seen from the above table, the anti-dandruff composition prepared in the embodiment of the present invention has an inhibition rate of TNF-α reaching more than 20%, an inhibition rate of 5α-reductase reaching more than 30%, and an inhibition rate of Malassezia reaching more than 60%. The extracts of Picea abies leaves, Hypericum perforatum, Salvia miltiorrhiza, and Olea europaea leaves synergistically with each other, and the prepared composition has a good effect of controlling oil and reducing dandruff.

[0152] By comparing Examples 1-12, it can be seen that the preparation methods of the Salvia miltiorrhiza root extract and the Salvia miltiorrhiza leaf extract, as well as the ratio of the Salvia miltiorrhiza root extract and the Salvia miltiorrhiza leaf extract, will affect the effect of controlling oil and reducing dandruff of the finally obtained anti-dandruff composition. By adopting the specific preparation method and ratio of the present invention, the finally obtained anti-dandruff composition has a better effect of controlling oil and reducing dandruff.

[0153] By comparing Example 1 with Examples 13-16, it can be seen that the weight parts of the components in the composition will affect the performance of the product. When the weight parts of the components are further selected within the preferred range, the obtained anti-dandruff composition has a better effect of controlling oil and reducing dandruff.

[0154] By comparing Example 1 with Comparative Examples 1-12, it can be seen that when one or two of the components are not added, or when other component parts are used to make up for the non-addition, the effects of the present invention cannot be achieved; and when other similar components are used for substitution and the weight parts of the components in the composition are not within the scope of the present application, good synergistic effects cannot be exerted.

[0155] Performance Test - 2

[0156] The present invention explores the application effects of the shampoos prepared in Application Examples 1-17, Comparative Application Examples 1-12, and Blank Application Example on the human body, including the following steps: Select a number of male volunteers aged 18-45 with a scalp condition of moderate dandruff and moderate scalp greasiness. The subjects sit quietly in a constant temperature and humidity room at a temperature of 21 ± 1°C and a humidity of 50 ± 10% for 30 min. The initial oil content is measured using a scalp oil tester Meibometer MB560, and the test site is selected on the top of the head; using a skin transdermal water loss probe TM Hex test the initial value of TEWL, and select the hairline as the test site; use a standard static-free comb to comb 100 times by a professional, collect dandruff, place the collected dandruff on a black board, take a photo, and use Image Pro Plus 7.0 to analyze the number of dandruff.

[0157] Randomly divide the subjects into 30 groups with 5 people in each group. Use the shampoo provided by the present invention once a day. Test the oil content, the number of dandruff, and the TEWL value at 4 weeks and 8 weeks, and analyze the improvement of each index of the volunteers at 4 and 8 weeks. The improvement is represented by the change rate of each index.

[0158] The calculation method is as follows:

[0159] The change rate % relative to the initial value = |(X after use - X before use) / X before use × 100%|; among them, the larger the change rate % relative to the initial value, the stronger the improvement effect.

[0160] Table 6

[0161]

[0162]

[0163] As can be seen from the above table, when the anti-dandruff composition prepared in the embodiment of the present invention is used to prepare shampoo, it has good effects of controlling oil, reducing dandruff, and repairing the scalp homeostasis.

[0164] From the comparison of Application Examples 1-12, it can be seen that when the prepared anti-dandruff composition is used to prepare shampoo, the preparation method of the salvia root extract and the salvia leaf extract in the composition, as well as the ratio of the salvia root extract and the salvia leaf extract, will affect the effects of the finally obtained anti-dandruff composition in controlling oil and reducing dandruff. By using the specific preparation method and ratio of the present invention, the finally obtained anti-dandruff composition has better effects of controlling oil and reducing dandruff.

[0165] From the comparison of Application Example 1 and Application Examples 13-16, it can be seen that when the prepared anti-dandruff composition is used to prepare shampoo, the weight parts of the components in the composition will affect the performance of the product. When the weight parts of the components are further selected within the preferred range, the obtained anti-dandruff composition has better effects of controlling oil and reducing dandruff.

[0166] From the comparison of Application Example 1 and Application Examples 1-12, it can be seen that when one or two components are not added, or when other component parts are supplemented while not adding, the effects of the present invention cannot be achieved when the prepared anti-dandruff composition is used to prepare shampoo; and when other similar components are used for substitution, when the weight parts of the components in the composition are not within the scope of the present application, good synergistic effects cannot be exerted.

[0167] Performance Test 3

[0168] The effect example of the present invention verifies the oil control, anti-dandruff and other effects of the product prepared in Application Example 1 on male consumers with sensitive skin, and specifically includes the following steps:

[0169] Thirty male subjects with sensitive skin were selected to participate in the test. The product was distributed to them. Each subject used the shampoo prepared in Application Example 1 every day for an 8-week application test. The consumers completed a questionnaire survey on the improvement degree of indicators such as oil control effect and anti-dandruff effect, and then stopped using the product; they switched to using the shampoo prepared in the blank application example and filled out the questionnaire survey on anti-dandruff effect again after 2 weeks and 4 weeks respectively. See Table 7 below for details.

[0170] Table 7

[0171]

[0172]

[0173] As can be seen from the above table, the product prepared by using the composition of the present invention can better maintain the microecological balance, and the dandruff problem will not recur after the product is discontinued.

[0174] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the protection scope of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present invention can be modified or equivalently replaced without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. An anti-dandruff composition, characterized in that, It comprises the following components in parts by weight: 0.5 - 5 parts of Norway spruce leaf extract, 0.1 - 2 parts of Hypericum perforatum extract, 0.1 - 2 parts of Salvia miltiorrhiza extract, and 0.5 - 5 parts of olive leaf extract; The Salvia miltiorrhiza extract is a mixture of Salvia miltiorrhiza root extract and Salvia miltiorrhiza leaf extract.

2. The anti-dandruff composition according to claim 1, wherein It comprises the following components in parts by weight: 1 - 3 parts of Norway spruce leaf extract, 0.5 - 1 part of Hypericum perforatum extract, 0.5 - 1 part of Salvia miltiorrhiza extract, and 1 - 2 parts of olive leaf extract.

3. The anti-dandruff composition according to claim 1, characterized in that, Based on the total weight of the antidandruff composition, the weight percentage content of the Salvia miltiorrhiza extract is 15 - 25%.

4. The anti-dandruff composition according to claim 1, wherein The weight ratio of the Salvia miltiorrhiza root extract to the Salvia miltiorrhiza leaf extract is 1:(1 - 3).

5. The anti-dandruff composition according to claim 1, characterized in that, The preparation method of the Salvia miltiorrhiza root extract comprises the following steps: S1. Add the dried, pulverized and sieved Salvia miltiorrhiza root into deionized water, adjust the pH to 3 - 4, then add a composite enzyme, which is a mixture of cellulase, pectinase and acid protease. After enzymatic hydrolysis, collect the enzymatic hydrolysis product; S2. Add an organic solvent to the enzymatic hydrolysis product for ultrasonic extraction, filter, collect the filtrate, and dry the filtrate by rotary evaporation to obtain the Salvia miltiorrhiza root extract.

6. The anti-dandruff composition according to claim 5, wherein, The weight ratio of the composite enzyme to the Salvia miltiorrhiza root is 1:(2 - 4). In the composite enzyme, the weight ratio of cellulase, pectinase and acid protease is cellulase:pectinase:acid protease = (1 - 3):(0.5 - 2):(0.1 - 0.5); and / or, the organic solvent is an ethanol solution with a volume fraction of 60 - 70%, and the weight ratio of the enzymatic hydrolysis product to the ethanol solution is 1:(20 - 40); and / or, the mesh number of the sieving is 800 - 1000 meshes, and the weight ratio of the Salvia miltiorrhiza root to deionized water is 1:(10 - 20); and / or, the temperature of the enzymatic hydrolysis is 40 - 45°C, and the time of the enzymatic hydrolysis is 20 - 30 min; after enzymatic hydrolysis, centrifuge to collect the enzymatic hydrolysis product, and the rotational speed of centrifugation is 3000 - 5000 r / min; and / or, the temperature of the ultrasonic extraction is 50 - 60°C, the time of the ultrasonic extraction is 15 - 30 min, and the ultrasonic power is 60 - 75 w; and / or, the temperature of the rotary evaporation is 40 - 50°C; and / or, the drying condition is vacuum drying at 50 - 60°C for 12 - 48 h; and / or, the enzyme activity of the cellulase is 20 - 50 U / mg, the enzyme activity of the pectinase is 20 - 50 U / mg, and the enzyme activity of the acid protease is 30 - 80 U / mg.

7. The anti-dandruff composition according to claim 1, characterized in that, The preparation method of the Salvia miltiorrhiza leaf extract comprises the following steps: (1) Dry and pulverize the Salvia miltiorrhiza leaves to obtain Salvia miltiorrhiza leaf powder; the mesh number of the Salvia miltiorrhiza leaf powder is 40 - 50 meshes; (2) Extract the Salvia miltiorrhiza leaf powder with an ethanol solution at 8 - 10 times the amount, collect the filtrate, and dry the filtrate by rotary evaporation to obtain the Salvia miltiorrhiza leaf extract.

8. The anti-dandruff composition according to claim 7, characterized in that, The volume fraction of the ethanol solution is 60 - 70%; and / or, the extraction is ultrasonic extraction, the temperature of the ultrasonic extraction is 55 - 65°C, the time of the ultrasonic extraction is 1 - 2 h, and the power of the ultrasonic extraction is 60 - 70 W; and / or, the temperature of the rotary evaporation is 40 - 50°C.

9. Use of an anti-dandruff composition according to any one of claims 1-8 in the preparation of a hair and skin care product.

10. A washing and care product, characterized in that, The hair and skin care product comprises an anti-dandruff composition according to any one of claims 1-8.

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