Recombinant collagen type VII with firming and anti-wrinkle efficacy

By preparing recombinant type VII collagen with the short amino acid sequence GLPGAKGEKGAP through multiple repetitions in the Pichia pastoris expression system, the problems of low secretion yield and insufficient firming and anti-wrinkle effects in commercial expression systems were solved, achieving high yield and excellent skin firming and anti-wrinkle effects.

CN120248038BActive Publication Date: 2026-06-09XIAN GIANT BIOGENE TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
XIAN GIANT BIOGENE TECH CO LTD
Filing Date
2025-04-10
Publication Date
2026-06-09

AI Technical Summary

Technical Problem

Existing recombinant collagen has low secretion yield in commercial expression systems and lacks firming and anti-wrinkle effects. It cannot effectively activate autophagy, improve endoplasmic reticulum stress, promote collagen secretion, or inhibit matrix metalloproteinase degradation.

Method used

Recombinant type VII collagen with high secretion in the Pichia pastoris expression system was prepared by repeated use of the short amino acid sequence GLPGAKGEKGAP. It exhibits excellent biological activities such as promoting autophagy, improving endoplasmic reticulum stress, promoting collagen secretion, and inhibiting matrix metalloproteinase degradation.

Benefits of technology

It has achieved high-yield industrial production of recombinant type VII collagen with a protein purity of over 90%, significantly improving skin firmness and anti-wrinkle effects, and enhancing skin elasticity and structural stability.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present patent application discloses a kind of recombinant collagen VII and its preparation method and use.The recombinant collagen VII is obtained by multiple repetitions of short amino acid sequence GLPGAKGEKGAP.The secretory yield of the recombinant collagen VII in commercialized pichia expression system can be as high as about 17.3g / L, the extracellular secreted protein is basically not degraded during fermentation process, and more than 90% purity can be achieved by simple ultrafiltration and decolorization, industrial production can be realized;Moreover, the recombinant collagen VII has more optimal biological activities of promoting autophagy, improving endoplasmic reticulum stress state, promoting collagen secretion and inhibiting matrix metalloproteinase degradation of collagen, and shows good firming and anti-wrinkle effects.
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