A strain of pristerophora adlerii fj08, microbial inoculum and preparation method and application thereof

Abstract

The present application belongs to the technical field of microorganisms, and specifically relates to a strain of Priestia aryabhattai FJ08, a microbial agent, a preparation method and application thereof, and can be used for inhibiting soil-borne diseases of radix pseudoginseng. The present application discloses a strain of Priestia aryabhattai FJ08, which is preserved in the Guangdong Microbial Digital Museum Center, has a preservation number of GDMCC No: 65907, and a preservation date of March 24, 2025. The Priestia aryabhattai FJ08 disclosed in the present application is isolated from radix pseudoginseng, is safe and reliable, has the abilities of inhibiting the growth of Fusarium oxysporum, producing IAA, producing ammonia, dissolving potassium and dissolving phosphorus, and can effectively inhibit the growth of pathogenic Fusarium oxysporum by using the Priestia aryabhattai FJ08 to soak radix pseudoginseng.

Description

Technical Field

[0001] This invention belongs to the field of microbial technology, specifically relating to a strain of Priestella auriculi FJ08, an inoculant, its preparation method, and its application. Background Technology

[0002] Prince ginseng, also known as child ginseng or baby ginseng, is the tuberous root of *Pseudostellaria heterophylla*, a perennial herbaceous plant belonging to the Caryophyllaceae family. First recorded in *Ben Cao Cong Xin* (New Compilation of Materia Medica), it is now listed as a medicinal herb suitable for use in health foods. Prince ginseng is a mild tonic, suitable for symptoms such as spleen and lung deficiency, qi and yin deficiency, and qi and yin deficiency. It contains various trace elements, cyclic peptides, amino acids, sugars, glycosides, phospholipids, fatty acids, sterols, and other chemical components. It has beneficial effects on improving myocardial ischemia, glucose and lipid metabolism in diabetes, and memory. Therefore, artificial cultivation of prince ginseng has promising applications.

[0003] However, serious soil-borne diseases often exist during the cultivation of *Pseudostellaria heterophylla*, leading to reduced yield and lower quality. These diseases severely restrict the development of the *Pseudostellaria heterophylla* industry. Currently, chemical control methods are commonly used to manage soil-borne diseases, but this method easily results in pesticide residues, affecting the safety and efficacy of the medicinal herb. Therefore, providing a safe and pollution-free method to effectively inhibit soil-borne diseases and suppress continuous cropping obstacles in *Pseudostellaria heterophylla* has become an urgent problem to be solved in this field. Summary of the Invention

[0004] The purpose of this invention is to provide a strain of *Priscilla argentea* FJ08, an inoculum agent, its preparation method, and its application. The *Priscilla argentea* FJ08 described in this invention can inhibit soil-borne diseases of *Pseudostellaria heterophylla*, suppress continuous cropping obstacles in *Pseudostellaria heterophylla*, and promote the growth of *Pseudostellaria heterophylla*. This invention specifically includes the following technical solutions.

[0005] A strain of *Priestia aryabhattai* FJ08, deposited at the Guangdong Provincial Center for Microbial Culture Collection (GDMCC No. 65907) on March 24, 2025.

[0006] The present invention also provides a microbial agent, wherein the effective component of the microbial agent includes the aforementioned *Priscilla argentea* FJ08.

[0007] Preferably, the viable count of *Priscilla argentea* FJ08 in the microbial agent is ≥4 × 10⁸. 8 CFU / mL.

[0008] The present invention also provides the application of *Priscilla argentea* FJ08 or microbial agents as described above in inhibiting soil-borne diseases of medicinal plants and / or promoting crop growth.

[0009] Preferably, promoting crop growth includes promoting crop seed germination and / or promoting crop seed growth.

[0010] Preferably, the plant includes a medicinal plant.

[0011] The present invention also provides a method for promoting plant growth by treating plant seeds with the microbial inoculant described above.

[0012] Preferably, the treatment method includes soaking the ginseng in the microbial agent.

[0013] Preferably, the soaking time is 30 to 60 minutes.

[0014] Preferably, the plant seeds include Codonopsis pilosula seeds.

[0015] Beneficial effects:

[0016] This invention provides a strain of *Priestia aryabhattai* FJ08, which is preserved at the Guangdong Provincial Center for Microbial Culture Collection (GDMCC No.: 65907) on March 24, 2025. This *Priestia aryabhattai* FJ08 was isolated from *Pseudostellaria heterophylla* seed ginseng, and is safe and reliable. It possesses the ability to produce IAA, ammonia, potassium, and phosphorus. Soaking *Pseudostellaria heterophylla* seed ginseng with *Priestia aryabhattai* FJ08 can effectively inhibit the growth of *Fusarium oxysporum*. Attached Figure Description

[0017] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the embodiments will be briefly described below.

[0018] Figure 1 This is a schematic diagram of the results of the plate confrontation experiment in Example 2;

[0019] Figure 2 The Gram staining results of *Priscilla argentea* FJ08 in Example 2;

[0020] Figure 3 The results of the starch hydrolysis test of *Priscilla argentea* FJ08 in Example 2;

[0021] Figure 4 The results of the nitrate reduction test of *Priscilla argentea* FJ08 in Example 2;

[0022] The left side represents the control group, and the right side represents the experimental group.

[0023] Figure 5 The results of the acetylmethyl methanol production test of *Priscilla argentea* FJ08 in Example 2;

[0024] The left side represents the control group, and the right side represents the experimental group.

[0025] Figure 6 The results of the ammonia production test of *Priscilla argentea* FJ08 in Example 2;

[0026] The left side represents the control group, and the right side represents the experimental group.

[0027] Figure 7 The results of the potassium solubility test for *Priscilla argentea* FJ08 in Example 2;

[0028] In the figure, K467 is a mark made for the experiment and has no special meaning;

[0029] Figure 8 The results of the experiment on Fusarium oxysporum infection of Codonopsis pilosula in Example 5;

[0030] The left side represents the control group, and the right side represents the experimental group.

[0031] Biological Preservation Instructions

[0032] Priestia aryabhattai FJ08, biologically classified as Priestia aryabhattai FJ08, was deposited on March 24, 2025, at the Guangdong Provincial Center for Microbial Culture Collection (GDMCC No. 65907). The deposit address is 100 meters west of No. 2, 102 Xianlie Middle Road, Yuexiu District, Guangzhou. Detailed Implementation

[0033] This invention provides *Priestia aryabhattai* FJ08, which is deposited at the Guangdong Provincial Microbial Culture Collection Center, located 100 meters west of No. 2, Xianlie Middle Road, Yuexiu District, Guangzhou, with accession number GDMCC No. 65907 and deposit date of March 24, 2025. The *Priestia aryabhattai* FJ08 of this invention exhibits the following characteristics on LB agar: flat, moist colonies that are round and pale yellow. *Priestia aryabhattai* FJ08 is a Gram-positive bacterium with the ability to hydrolyze starch, reduce nitrates, decompose glucose, produce ammonia, and dissolve potassium.

[0034] The present invention also provides a microbial inoculant, including *Priscilla argentea* FJ08 as described above.

[0035] As one embodiment, the viable count of *Priscilla argentea* FJ08 in the microbial agent of the present invention is ≥4 × 10⁻⁶. 8 CFU / mL. As another embodiment, the viable count of *Priscilla auriculata* FJ08 in the microbial agent of the present invention can be 4 × 10⁻⁶. 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL and 9×10 8 Any of the following concentrations: CFU / mL. In a specific embodiment, the viable count of *Priscilla auriculata* FJ08 in the microbial agent of the present invention is 4 × 10⁻⁶. 8 CFU / mL.

[0036] The present invention also provides a method for preparing the microbial inoculant as described above, comprising the following steps:

[0037] Priestella auriculi FJ08 was inoculated into LB liquid medium and cultured until the OD of the contents of the LB liquid medium reached a certain level. 600 After the value is 0.5 to 0.6, a bacterial solution is obtained; the bacterial solution is centrifuged for the first time, the supernatant is removed, sterile water is added, and centrifuged again to remove the supernatant and obtain a precipitate; sterile water is added to the precipitate and mixed to obtain the microbial inoculant.

[0038] This invention involves inoculating *Priscilla auriculata* FJ08 into LB liquid medium and culturing it until the OD of the contents of the LB liquid medium reaches a certain level. 600 After the value is set to 0.5–0.6, the bacterial culture is obtained. As one embodiment, the OD of the contents of the LB liquid culture medium... 600 The value can be any one of 0.5, 0.51, 0.52, 0.53, 0.54, 0.55, 0.56, 0.57, 0.58, 0.59 and 0.6.

[0039] After obtaining the bacterial culture, the present invention centrifuges the bacterial culture for the first time, removes the supernatant, adds sterile water, centrifuges again, removes the supernatant, and obtains a precipitate. In one embodiment, the specific operation of the first and second centrifugations in the present invention is centrifugation at 1000 rpm for 3-7 minutes. In another embodiment, the duration of the first and second centrifugations in the present invention can be any one of 3 minutes, 4 minutes, 5 minutes, 6 minutes, and 7 minutes. In one embodiment, the amount of sterile water added can be 0.5-1.5 mL. In another embodiment, the amount of sterile water added can be any one of 0.5 mL, 0.6 mL, 0.7 mL, 0.8 mL, 0.9 mL, 1.0 mL, 1.1 mL, 1.2 mL, 1.3 mL, 1.4 mL, and 1.5 mL. In the present invention, the bacterial culture is centrifuged at 1000 rpm for 5 minutes for the first centrifugation, the supernatant is removed, 1 mL of sterile water is added, and centrifuged again at 1000 rpm for 5 minutes, the supernatant is removed, and a precipitate is obtained.

[0040] After obtaining the precipitate, the present invention adds sterile water to the precipitate and mixes it to obtain the microbial inoculant. As one embodiment, the precipitate is mixed with sterile water until the effective concentration of *Priscilla argentea* FJ08 in the microbial inoculant is ≥4 × 10⁻⁶. 8 CFU / mL. As another embodiment, the effective concentration of the microbial strain in the microbial agent of the present invention can be 4 × 10⁻⁶ CFU / mL. 8 CFU / mL, 5×10 8 CFU / mL, 6×10 8 CFU / mL, 7×10 8 CFU / mL, 8×10 8 CFU / mL and 9×10 8 Any of the following concentrations: CFU / mL. In a specific embodiment, the effective concentration of the bacterial strain in the microbial agent of the present invention is 4 × 10⁻⁶. 8 CFU / mL.

[0041] The present invention also provides the application of the *Priscilla argentea* FJ08 strain, microbial inoculant, or microbial inoculant prepared by the above-described method in inhibiting soil-borne diseases of crops, inhibiting crop continuous cropping obstacles, and promoting crop growth, or in any one or more of these applications.

[0042] In one embodiment, the crop includes a medicinal crop; the medicinal crop includes *Pseudostellaria heterophylla*. In another embodiment, the soil-borne diseases of the crop include soil-borne diseases caused by *Fusarium oxysporum*.

[0043] This invention also provides a method for inhibiting soil-borne crop diseases, suppressing crop continuous cropping obstacles, and / or promoting crop growth, by soaking crop seeds in a microbial agent as described above. In one embodiment, the soaking time is 30–60 minutes. In another embodiment, the soaking time can be any one of 30 minutes, 35 minutes, 40 minutes, 45 minutes, 50 minutes, 55 minutes, and 60 minutes.

[0044] To further illustrate the present invention, the following detailed description, in conjunction with the accompanying drawings and embodiments, provides a strain of Priestella auriculi FJ08, an inoculum agent, its preparation method, and its application. However, these descriptions should not be construed as limiting the scope of protection of the present invention.

[0045] Example 1: Isolation, screening and identification of Priscilla auriculi FJ08

[0046] In this embodiment, *Pseudostellaria heterophylla* seed ginseng was collected from a *Pseudostellaria heterophylla* planting area in Zherong County, Fujian Province. 5g of seed ginseng was weighed and placed in an Erlenmeyer flask, 45mL of sterile water was added, and the flask was placed in a constant-temperature shaker and shaken thoroughly for 30 minutes to obtain 10... -1 Diluent.

[0047] Will contain 10 -1 The conical flask containing the diluent was heated in a 75°C water bath for 30 minutes. 5 mL of the 10... -1 The diluent was transferred to another Erlenmeyer flask containing 45 mL of sterile water to obtain 10 -2 Diluent. Following the above procedure, prepare 10... -3 Diluent, 10 -4 Diluent.

[0048] 50 μL of each dilution was evenly spread onto LB medium and incubated at 37°C for 12 hours. After incubation, the microbial growth at each dilution was observed, and the 10 dilutions with the best growth were selected. -2 Using the dilution solution as the optimal dilution, select all single colonies on the plate and transfer them to a new plate for further incubation. The incubation time is about 10 hours. When the colonies are clearly visible, the isolated and screened bacteria are obtained and stored briefly in a 4°C refrigerator for later use.

[0049] The isolated bacteria were molecularly identified using PCR amplification of the 16S–23S rRNA gene region. The primer sequences are shown in SEQ ID NO:1 and SEQ ID NO:2, and the sequencing sequences are shown in SEQ ID NO:3.

[0050] 27F (SEQ ID NO: 1): 5′-AGAGTTTGATCCTGGCTCAG-3′;

[0051] 1492R(SEQ ID NO:2):5′-TACGACTTAACCCCAATCGC-3′;

[0052] SEQ ID NO:3:

[0053]

[0054] The PCR reaction system (25 μl) consisted of: 1 μl each of forward and reverse primers, 2 μl of template, 8.5 μl of enzyme-free water, and 12.5 μl of mix.

[0055] The PCR reaction program was 94℃ denaturation for 1 min, 55℃ annealing for 45 s, 72℃ extension for 90 s, for 35 cycles. Sequencing sequences were compared and analyzed against the NCBI database using BLAST.

[0056] Molecular biological identification confirmed that the bacterium was *Priestiaaryabhattai*, named *Priestiaaryabhattai* FJ08. The isolated strain was deposited at the Guangdong Provincial Center for Microbial Culture Collection on March 24, 2025, with accession number GDMCC No: 65907.

[0057] Upon observation of the *Priscilla argentea* FJ08, it was observed that the *Priscilla argentea* FJ08 formed flat, moist colonies on LB solid medium, and the colonies exhibited a metallic sheen.

[0058] Example 2: Physiological and biochemical characteristics of *Priscilla argentea* FJ08

[0059] The selected *Priscilla argentea* FJ08 was transferred to LB liquid medium for expansion culture to obtain *Priscilla argentea* FJ08 culture medium. Gram staining test, starch hydrolysis test, nitrate reduction test, acetylmethylmethanol production test and ammonia production test were performed in sequence.

[0060] (1) Flat plate confrontation experiment

[0061] Using a sterilized white pipette tip, collect pathogenic *Fusarium oxysporum* hyphae (disclosed in the article "Liu YT, Zhang YX, Wang XY, et al. *Pseudostellaria heterophylla* cultivar mixtures driven changes in rhizosphere metabolites to suppress soil-borne Fusarium disease[J]. Agriculture Ecosystems & Environment, 2025, 380.") and spot them in the center of a petri dish containing PDA medium. Incubate at 37°C for 10 hours. Using a sterilized white pipette tip, collect *Burkholderia gravidarum* SJ08 and draw two 1cm horizontal lines 1cm apart from the center of the medium. Incubate at 37°C for 24 hours and observe the growth of *Fusarium oxysporum* and *Burkholderia gravidarum* SJ08. The results are as follows: Figure 1As can be seen, the Burkhold SJ08 strain provided by this invention can significantly inhibit the growth of pathogenic Fusarium hyphae.

[0062] (2) Gram staining test

[0063] Dip an inoculation loop in *Priscilla argentea* FJ08 culture medium and smear it directly onto a glass slide. Hold the slide on the back of the specimen and swing it three times at pendulum speed through the hottest part of an alcohol lamp flame to fix the bacteria onto the slide. Add a few drops of crystal violet stain to the fixed bacterial smear and incubate at room temperature for 1 minute. Gently rinse with a fine stream of water, then add a few drops of iodine mordant and incubate at room temperature for 1 minute. Rinse again with a fine stream of water. After rinsing, add a few drops of 95% alcohol and gently shake the slide for 5 seconds to ensure even destaining. Then, hold the slide at an angle to allow the destaining dye to run off with the alcohol. Add more alcohol until the flowing alcohol is colorless or slightly pale purple. Immediately rinse off the alcohol with a fine stream of water. Counterstain with safranin for 2 minutes, rinse with water, and after staining, air dry the specimen. Observe under a microscope. See [link to results]. Figure 2 .according to Figure 2 It can be seen that *Priscilla argentea* FJ08 is purple, indicating that *Priscilla argentea* FJ08 is a Gram-positive bacterium.

[0064] (3) Starch hydrolysis test

[0065] Prepare the starch hydrolysis medium according to the components shown in Table 1.

[0066] Table 1. Preparation of starch hydrolysis medium

[0067]

[0068] Priestella auriculi FJ08 was inoculated into starch hydrolysis medium and cultured at 37°C for 24 hours. Iodine solution was added dropwise to the medium, ensuring complete coverage. The experimental phenomena were observed under light. The results are shown in [reference needed]. Figure 3 .according to Figure 3 The presence of a colorless transparent ring around *Priscilla argentea* FJ08 indicates that *Priscilla argentea* FJ08 has the ability to hydrolyze starch.

[0069] (4) Nitrate reduction test

[0070] Prepare Griselt reagent according to the components shown in Table 2.

[0071] Table 2. Preparation of Griselda Reagent

[0072]

[0073] *Priscilla auriculata* FJ08 was inoculated into nitrate-reducing liquid medium as the experimental group, while an uninoculated medium was prepared as the control group. Both groups were incubated together at 37°C for 48 hours. After incubation, 50 μl each of Gliese's reagent I and II were added to the culture media of the experimental and control groups, respectively. The results are shown below. Figure 4 ,according to Figure 4 It can be seen that the culture medium in the control group showed no change, while the culture medium in the experimental group turned red, indicating that *Priscilla argentea* FJ08 can reduce nitrate to nitrite, ammonia, and nitrogen.

[0074] (5) Acetylmethylmethanol formation test

[0075] Priestella auriculi FJ08 was inoculated into glucose peptone medium as the experimental group, while uninoculated glucose peptone medium was prepared as the control group. Both the control group and the experimental group were placed in an incubator at 37°C for 4 days.

[0076] After the culture medium for the control and experimental groups was removed, equal volumes of 40% (w / v) KOH were added to the culture medium in both groups, followed by 0.5 mg of creatine. The mixture was then shaken, and the phenomena were observed within 10 minutes. If bacteria in glucose-peptone aqueous culture medium can decompose glucose to produce pyruvate, the pyruvate condenses and decarboxylates to acetylmethylethanol. In a strongly alkaline environment, the acetylmethylethanol is oxidized by oxygen in the air to diacetyl. Diacetyl reacts with the guanidinium group in peptone to form a red compound, known as the VP(+) reaction. See the results below. Figure 5 ,according to Figure 5 It can be seen that the culture medium in the experimental group turned red, which is a positive reaction, indicating that *Priscilla argentea* FJ08 has the ability to decompose glucose.

[0077] (6) Ammonia production test

[0078] The ammonia production test medium was prepared according to the components shown in Table 3.

[0079] Table 3. Preparation of culture medium for ammonia production determination.

[0080]

[0081] *Priscilla auriculata* FJ08 was inoculated into ammonia production assay medium. A separate ammonia production assay medium without inoculation was prepared as a control. Both the control and experimental groups were incubated together at 37°C for 24 hours. After incubation, 5 ml of sodium reagent was added to each of the control and experimental groups, and the mixture was gently shaken. Figure 6 It can be seen that the experimental group produced an orange-red precipitate, which is a positive reaction, indicating that *Priscilla argentea* FJ08 has the ability to produce ammonia.

[0082] (7) Potassium dissolution test

[0083] Prepare potassium-soluble culture medium according to the components shown in Table 4.

[0084] Table 4 Preparation of potassium-soluble culture medium

[0085]

[0086]

[0087] Priestella auriculi FJ08 was inoculated into potassium-soluble medium and incubated at 37°C for 48 hours. Results are shown below. Figure 7 ,according to Figure 7 The presence of a colorless transparent ring around *Priscilla argentea* FJ08 indicates that *Priscilla argentea* FJ08 has the ability to dissolve potassium.

[0088] Example 3: A method for preparing Priscilla argentea FJ08 bacterial suspension

[0089] Priestella auriculi FJ08 was inoculated into 800 μL LB liquid and cultured for 18 h until OD was reached. 600 After adjusting the concentration to 0.5–0.6, a culture medium was obtained. The culture medium was centrifuged at 1000 rpm for 5 minutes, the supernatant was discarded, 1 mL of sterile water was added, and the mixture was shaken well. It was then centrifuged again for 5 minutes, and the supernatant was discarded again to wash away any remaining LB. A precipitate was obtained, and 5 mL of sterile water was added to the precipitate and shaken well to prepare the bacterial suspension. The concentration of *Priscilla argentea* FJ08 in the bacterial suspension was 4.0 × 10⁻⁶. 8 cfu / mL.

[0090] Example 4: A method for inhibiting soil-borne diseases and continuous cropping obstacles in *Pseudostellaria heterophylla* and promoting its growth.

[0091] Pretreatment of ginseng seedlings: Wash the ginseng seedlings with clean water to remove surface dirt, disinfect them with 2% sodium hypochlorite solution for 1 minute, rinse them 5 times with distilled water, then disinfect them with 75% ethanol for 2 minutes, rinse them 3 times with distilled water, and then blot the surface moisture with filter paper.

[0092] Soak the ginseng seedlings in the *Priscilla argentea* FJ08 bacterial solution as described in Example 2 for 1 hour.

[0093] Example 5: Verification of the antibacterial effect of *Priscilla argentea* FJ08

[0094] In this embodiment, 12 ginseng seeds were taken and divided into 4 groups on average.

[0095] Treatment 1: Take 3 Codonopsis pilosula seed ginseng and immerse them simultaneously in the Priestella auriculi FJ08 bacterial solution prepared by the preparation method described in Example 3. After soaking for 60 minutes, remove the seed ginseng.

[0096] Treatment 2 (CK group): Three seed ginseng were immersed in sterile water as the CK control and removed after soaking for 60 minutes.

[0097] Treatment 3: Take three *Pseudostellaria heterophylla* seed ginseng and soak them in SJ06 bacterial agent for 60 minutes, then remove the ginseng. The preparation method of the SJ06 bacterial agent is the same as in Example 3, except that the SJ06 strain is used for preparation. SJ06 is another strain screened from *Pseudostellaria heterophylla* seed ginseng by the applicant in previous experiments according to the method described in Example 1.

[0098] Treatment 4: Take 3 *Pseudostellaria heterophylla* seed ginseng and soak them in FJ11 bacterial agent for 60 minutes, then remove the ginseng. The preparation method of the FJ11 bacterial agent is the same as in Example 3, except that the FJ11 strain is used for preparation. The FJ11 is another strain screened from *Pseudostellaria heterophylla* seed ginseng by the applicant in previous experiments according to the method described in Example 1.

[0099] Using a sterile scalpel, 0.5 cm incisions were made on the surface of the ginseng seedlings after soaking in the different treatments described above. Fusarium oxysporum hyphae were then applied to the wound surface using a sterile white pipette tip. The ginseng seedlings were placed in a petri dish lined with sterile filter paper, and 4 mL of sterile water was added to keep them moist. The petri dish was then placed in a biochemical incubator and incubated at 37°C. The growth of Fusarium oxysporum on the wound surface of the ginseng seedlings was observed. The results are as follows: Figure 8 As shown in Table 5, the lengths of *Fusarium oxysporum*-infected mycelial blocks were counted at 24, 36, and 48 hours after inoculation.

[0100] Table 5. Length (cm) of Fusarium oxysporum colonies at different time points.

[0101] Processing group / time (h) 24 36 48 Process 2 (CK) 0.5 0.8 1.23 Process 1 0.44 0.58 0.75 Process 3 0.48 0.73 1.05 Process 4 0.48 0.75 1.14

[0102] Depend on Figure 8 As shown in Table 5, compared with the control group, the ginseng treated with Priestella auriculi FJ08 had smaller wound surfaces and shorter Fusarium oxysporum clumps after being infected with pathogenic Fusarium oxysporum, indicating that the bacterium has a good antibacterial effect.

[0103] In summary, this invention provides a strain of *Priscilla argentea* FJ08. The bacterial solution prepared using *Priscilla argentea* FJ08 described in this invention has a good inhibitory effect on soil-borne diseases of *Codonopsis pilosula*, can effectively inhibit crop diseases caused by *Fusarium oxysporum*, can also inhibit crop continuous cropping obstacles, promote crop growth, and is safe and pollution-free, showing good application prospects.

[0104] Although the above embodiments have provided a detailed description of the present invention, they are only some embodiments of the present invention, and not all embodiments. People can obtain other embodiments based on these embodiments without creative effort, and these embodiments all fall within the protection scope of the present invention.

Claims

1. A strain of Priestella auriculi ( Priestia aryabhattai FJ08, characterized in that, The *Priestella auriculata* FJ08 strain is preserved at the Guangdong Provincial Center for Microbial Culture Collection, with accession number GDMCCNo: 65907, and the preservation date is March 24, 2025.

2. A microbial inoculant, characterized in that, The active ingredient of the microbial agent includes *Priscilla auriculata* FJ08 as described in claim 1.

3. The microbial agent according to claim 2, characterized in that, The viable count of *Priscilla argentea* FJ08 in the microbial agent is ≥4 × 10⁸. 8 CFU / mL.

4. The application of *Priestella auriculi* FJ08 as described in claim 1 or the microbial agent as described in claim 2 or 3 in inhibiting soil-borne diseases of medicinal crops and / or promoting crop growth; wherein the inhibition of soil-borne diseases of medicinal crops is the inhibition of soil-borne diseases of *Codonopsis pilosula* caused by *Fusarium oxysporum*; wherein the crop includes medicinal crops; and wherein the medicinal crops include *Codonopsis pilosula*.

5. The application according to claim 4, characterized in that, The promotion of crop growth includes promoting crop seed germination and / or promoting crop seed growth.

6. A method for promoting plant growth, characterized in that, Plant seeds are treated with the microbial inoculant according to any one of claims 2 to 3, wherein the plant seeds include Codonopsis pilosula seeds.

7. The method according to claim 6, characterized in that, The treatment method includes soaking the ginseng in the microbial agent.

8. The method according to claim 7, characterized in that, The soaking time is 30-60 minutes.

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