A cream for promoting pet hair growth and its preparation method

Abstract

This application relates to a cream for promoting pet hair growth and its preparation method, belonging to the technical field of pet biomedicine. It comprises the following raw materials in weight percentages: microencapsulated fish oil 8%-10%; hemoglobin peptide 6%-12%; silkworm pupa protein powder 3%-5%; hydrolyzed chicken liver powder 2%-5%; yeast extract 1%-3%; rosemary extract 0.005%-0.01%; and antioxidant 0.1%-0.4%. The hemoglobin peptide is selected from one or more of MLL peptides and WDL peptides. The amino acid sequence of the MLL peptide is methionine-leucine-leucine; and the amino acid sequence of the WDL peptide is tryptophan-aspartic acid-leucine. This application has synergistic and long-term stable effects, is suitable for dogs, cats, and other pets, is safe with no side effects, and can be mass-produced.

Description

Technical Field

[0001] This application relates to the field of pet biomedical technology, and in particular to a cream that promotes pet hair growth and its preparation method. Background Technology

[0002] Pet coat health is a crucial indicator of their overall nutritional status and skin function. Coat quality not only affects a pet's appearance but is also closely related to skin barrier integrity, immune regulation, and microcirculation. In recent years, with the increasing penetration rate of pet households (the global pet care market has achieved a CAGR of 6.8%), consumer demand for pet coat care products has shifted from basic cleaning to functional and precise solutions, with particular focus on hair loss repair, hair follicle activation, and anti-inflammatory soothing effects. However, existing technologies still have significant shortcomings in ingredient design, mechanisms of action, and long-term safety, specifically:

[0003] Limited ingredient diversity and lack of synergistic effects: Most commercially available products rely on a single active ingredient (such as Omega-3, Vitamin E, or Biotin in fish oil). While these can partially improve hair shine, they cannot systematically address deeper issues such as hair follicle atrophy and microcirculation disorders. For example, fish oil creams promote hair growth only by supplementing unsaturated fatty acids, but experiments show that their transdermal absorption rate is less than 15%, and they have no significant effect on the proliferation of hair follicle stem cells. Furthermore, single ingredients are prone to oxidative inactivation (e.g., the peroxide value of fish oil increases by more than 30% during storage), leading to poor product stability.

[0004] Limitations of the application of blood peptides: Although blood-derived bioactive peptides (such as blood peptide concentrate) have shown potential in the field of human hair growth, their application in pet care is still in its early stages.

[0005] Transdermal delivery systems are inefficient: Pet skin structure differs significantly from human skin (e.g., slightly neutral pH, complex lipid composition). While traditional cream bases (such as petrolatum) can moisturize, they hinder the penetration of active ingredients. Furthermore, chemical penetration enhancers (such as propylene glycol) may cause skin irritation in pets (Draize score ≥2), limiting long-term use.

[0006] Therefore, there is an urgent need in this field to develop a cream that promotes pet hair growth to fill the gap in precision pet hair follicle repair technology. Summary of the Invention

[0007] The purpose of this application is to provide a cream that promotes pet hair growth and its preparation method. The cream of this application, through scientific formulation and advanced preparation process, has the functions of nutritional supplementation, anti-oxidation and hair follicle activation. It is suitable for pets such as dogs and cats, is safe and has no side effects, and can be mass-produced.

[0008] In a first aspect, this application provides a cream for promoting pet hair growth, comprising the following raw materials by weight percentage:

[0009] Microencapsulated fish oil 8%-10%;

[0010] Blood protein peptides: 6%-12%;

[0011] Silkworm pupa protein powder 3%-5%;

[0012] Hydrolyzed chicken liver powder 2%-5%;

[0013] Yeast extract 1%-3%;

[0014] Rosemary extract 0.005%-0.01%;

[0015] Antioxidant 0.1%-0.4%;

[0016] The hemoprotein peptide is selected from one or more of MLL peptide and WDL peptide; the amino acid sequence of the MLL peptide is methionine-leucine-leucine; and the amino acid sequence of the WDL peptide is tryptophan-aspartic acid-leucine.

[0017] Optionally, the hemoprotein peptide is composed of MLL peptide and WDL peptide.

[0018] Optionally, the antioxidant consists of d-biotin and vitamin E.

[0019] Optionally, the cream may further include the following raw materials by weight percentage:

[0020] Oil phase matrix 10%-20%;

[0021] Aqueous matrix 60%-70%;

[0022] Emulsifier 3%-6%.

[0023] Optionally, the oil phase matrix is ​​composed of glyceryl monostearate and white petrolatum.

[0024] Optionally, the aqueous matrix is ​​composed of carbomer, glycerol and water.

[0025] Optionally, the emulsifier is one or more of sodium dodecyl sulfate, octanoic acid-capric acid-decalcium polyethylene glycol glycerol, and lauroyl polyoxyethylene (6) glycerol ester.

[0026] Secondly, this application provides a method for preparing a cream that promotes pet hair growth, comprising the following steps:

[0027] S1: Oil phase preparation: Glyceryl monostearate, white petrolatum, and microencapsulated fish oil are heated and melted;

[0028] S2: Aqueous phase preparation: Dissolve sodium dodecyl sulfate, carbomer, and glycerol in water and mix by heating;

[0029] S3: Preparation of active phase: Dissolve hemoglobin peptides in water and homogenize them with hydrolyzed chicken liver powder and silkworm pupa protein powder;

[0030] S4: Cream preparation: Slowly add the oil phase to the aqueous phase, stir evenly, cool down, add the active phase, yeast extract, antioxidant and rosemary extract, homogenize, adjust the pH to 6.5-7.0, homogenize and fill to obtain the finished product.

[0031] In summary, this application includes at least one of the following beneficial technical effects:

[0032] 1. Synergistic effect: Microencapsulated fish oil (rich in Omega-3) combined with blood peptide concentrate (promotes nutrient absorption in hair follicles) enhances hair follicle activity;

[0033] 2. Natural and safe: Silkworm pupa protein powder and hydrolyzed chicken liver powder provide essential amino acids, and rosemary extract replaces chemical preservatives;

[0034] 3. High stability: Microencapsulation technology protects the active ingredients, and vitamin E and d-biotin synergistically delay oxidation. No crystallization occurs after 12 months of storage at room temperature. Detailed Implementation

[0035] The present invention will be further described below with reference to specific embodiments.

[0036] Preparation example: hemoprotein peptides

[0037] Blood protein peptides were screened from bovine blood protein solution after enzymatic hydrolysis, and identified as WDL (TrpAspLeu) and MLL (MetLeuLeu) peptides. These peptides were synthesized separately using an automated peptide synthesizer via solid-phase synthesis (by Nanjing Yuantai Biotechnology Co., Ltd.). The peptides were purified by high-performance liquid chromatography (HPLC), and identified using mass spectrometry (MS) and amino acid sequence analysis to confirm their sequence and purity. Finally, blood protein peptides with a purity greater than 95% were obtained.

[0038] Example 1: A cream for promoting pet hair growth

[0039] The raw material composition of a cream for promoting pet hair growth is as follows: microencapsulated fish oil: 8%; MLL hemoglobin peptide: 1%; WDL hemoglobin peptide: 1%; silkworm pupa protein powder: 5%; hydrolyzed chicken liver powder: 4%; yeast extract: 2%; d-biotin: 0.06%; vitamin E: 0.34%; rosemary extract: 0.01%; oil phase matrix glyceryl monostearate 2%; oil phase matrix white petrolatum 8%; emulsifier sodium lauryl sulfate 4%; aqueous phase matrix carbomer 2%; aqueous phase matrix glycerin 3%; the remainder is aqueous phase matrix water, to a total of 100%.

[0040] The preparation method of the above-mentioned cream includes the following steps:

[0041] S1: Oil phase preparation: Glyceryl monostearate, white petrolatum, and microencapsulated fish oil were heated to 70°C to melt;

[0042] S2: Aqueous phase preparation: Dissolve sodium dodecyl sulfate, carbomer, and glycerol in water and heat to 50°C to mix;

[0043] S3: Preparation of active phase: Dissolve MLL peptide and WDL peptide in water and homogenize with hydrolyzed chicken liver powder and silkworm pupa protein powder;

[0044] S4: Cream preparation: Slowly add the oil phase to the aqueous phase, stir at 1300 r / min for 20 minutes, cool to 40℃, then add the active phase, yeast extract, d-biotin, vitamin E and rosemary extract, homogenize, adjust the pH to 6.5-7.0, homogenize and fill to obtain the finished product.

[0045] Example 2: A cream for promoting pet hair growth

[0046] The raw material composition of a cream for promoting pet hair growth is as follows: microencapsulated fish oil: 10%; MLL hemoglobin peptide: 3%; WDL hemoglobin peptide: 3%; silkworm pupa protein powder: 3%; hydrolyzed chicken liver powder: 2%; yeast extract: 1%; d-biotin: 0.015%; vitamin E: 0.085%; rosemary extract: 0.005%; oil phase matrix glyceryl monostearate 2%; oil phase matrix white petrolatum 8%; emulsifier sodium lauryl sulfate 4%; aqueous phase matrix carbomer 2%; aqueous phase matrix glycerin 3%; the remainder is aqueous phase matrix water, to a total of 100%.

[0047] The preparation method is the same as in Example 1.

[0048] Comparative Example 1: A cream that promotes pet hair growth

[0049] The raw material composition of a cream for promoting pet hair growth is as follows: silkworm pupa protein powder: 3%; hydrolyzed chicken liver powder: 2%; yeast extract: 1%; d-biotin: 0.015%; vitamin E: 0.085%; rosemary extract: 0.005%; oil phase matrix glyceryl monostearate 2%; oil phase matrix white petrolatum 8%; emulsifier sodium lauryl sulfate 4%; aqueous phase matrix carbomer 2%; aqueous phase matrix glycerin 3%; the remainder is aqueous phase matrix water, to a total of 100%.

[0050] The preparation method is the same as in Example 1.

[0051] Example 3: Long-term stability study of the cream

[0052] Experimental conditions:

[0053] High temperature test: Placed in a 60℃ constant temperature chamber for 10 days, samples were taken for testing on days 0, 5 and 10.

[0054] High humidity test: Placed for 10 days under a relative humidity of 90% ± 5%, with the same sampling time as above.

[0055] Strong light exposure experiment: Placed under a light intensity of 4500lx±500lx for 10 days, with the sampling time as above.

[0056] Long-term experiment: Stored at 25℃±2℃ and relative humidity of 60%±10% for 12 months, and samples were taken for testing at the end of 0, 3, 6, 9 and 12 months.

[0057] Testing indicators:

[0058] Physical stability: properties (color, uniformity), stratification, crystallization phenomenon.

[0059] Chemical stability: MLL / WDL peptide content (HPLC method, C18 column, mobile phase: 0.1M phosphate buffer-acetonitrile gradient elution), vitamin E oxidation rate (iodometry).

[0060] The experimental results are shown in Table 1:

[0061] Table 1

[0062]

[0063] in conclusion:

[0064] The MLL / WDL peptide content retention rate is >95%, and the vitamin E oxidation rate is <5%; the cream remains stable for 12 months under normal storage conditions (25℃) and meets pharmaceutical standards.

[0065] Example 4: Animal efficacy experiment

[0066] C57BL / 6 alopecia mice (dexamethasone-induced alopecia model) were used and divided into 4 groups (n=10): control group: blank control group (comparative example 1), low-dose group: cream containing 3% each of MLL and WDL peptides (Example 1), high-dose group: cream containing 6% each of MLL and WDL peptides (Example 2), and positive control group: commercially available fish oil cream. Administration: Topical application once daily for 6 consecutive weeks. Detection indicators: hair regeneration rate: percentage of skin surface covered by hair; hair follicle density: number of hair follicles per square millimeter counted by histological section staining (H&E).

[0067] The experimental results are shown in Table 2:

[0068] Group Hair regrowth rate <![CDATA[Hair follicle density (number / mm 2 )]]> Blank control group 12％±3％ 8.2±1.5 low-dose group 58％±7％ 22.4±3.1* High-dose group 82％±9％ 34.6±4.5** Positive control group 32％±5％ 12.3±2.3

[0069] *Note: **P<0.05, *P<0.01 (compared to the blank control group)

[0070] Conclusion: The high-dose group showed an 82% increase in hair regrowth rate and a 3.2-fold increase in hair follicle density, which was significantly better than commercially available products, confirming that the cream has the function of promoting hair follicle repair.

[0071] Those skilled in the art will further recognize that the invention can be embodied in other specific forms without departing from its spirit or central characteristics. Since the foregoing description of this disclosure only discloses exemplary embodiments therein, it should be understood that other variations are considered to be within the scope of the invention. Therefore, the invention is not limited to the specific embodiments described in detail herein. Rather, reference should be made to the appended claims to indicate the scope and content of the invention.

Claims

1. A cream for promoting hair growth in a pet, characterized by, The preparation raw materials include the following mass percentages: Microencapsulated fish oil 8%-10%; Blood protein peptide 6%-12%; Silkworm chrysalis protein powder 3%-5%; Hydrolyzed chicken liver powder 2%-5%; Yeast extract 1%-3%; Rosemary extract 0.005%-0.01%; Antioxidant 0.1%-0.4%; The blood protein peptide is composed of an MLL peptide segment and a WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine-leucine-leucine; and the amino acid sequence of the WDL peptide segment is tryptophan-aspartic acid-leucine.

2. The cream for promoting hair growth of a pet according to claim 1, characterized by, The antioxidant is composed of d-biotin and vitamin E.

3. The cream for promoting hair growth of a pet according to claim 1, characterized by, The cream further includes the following mass percentages of preparation raw materials: Oil phase matrix 10%-20%; Water phase matrix 60%-70%; Emulsifier 3%-6%.

4. The cream for promoting hair growth of a pet according to claim 3, characterized by, The oil phase matrix is composed of glyceryl monostearate and white vaseline.

5. The cream for promoting hair growth of a pet according to claim 3, characterized by, The water phase matrix is composed of carbomer, glycerol and water.

6. The cream for promoting hair growth of a pet according to claim 3, wherein The emulsifier is one or several of sodium dodecyl sulfate, caprylocaproyl macrogol glycerides and lauryl polyoxyethylene (6) glycerides.

7. A process for the preparation of a cream as claimed in any one of claims 1 to 6, characterised in that, The method includes the following steps: S1: oil phase preparation: heat and melt glyceryl monostearate, white vaseline and microencapsulated fish oil; S2: water phase preparation: dissolve sodium dodecyl sulfate, carbomer and glycerol in water and heat and mix; S3: active phase preparation: dissolve blood protein peptide in water and homogenize with hydrolyzed chicken liver powder and silkworm chrysalis protein powder; S4: cream preparation: slowly add the oil phase to the water phase, stir uniformly, add the active phase, yeast extract, antioxidant and rosemary extract after cooling, homogenize, adjust the pH to 6.5-7.0, homogenize and fill, and the finished product is obtained.

Images