Bactrocera cucurbitae and fruit fly repellent and application thereof

Abstract

The invention relates to the field of pesticide preparations, and discloses a bactrocera cucurbitae and South Asia fruit fly repellent and application thereof.The repellent is a preparation with phenylacetaldehyde as an effective component, a phenylacetaldehyde solvent in the repellent is ultrapure water or dimethyl sulfoxide, and the content of phenylacetaldehyde in the repellent is 0.005-0.2% by weight. The phenylacetaldehyde repellent can significantly reduce the oviposition behaviors of the two fruit flies on host plants, so that fruits are effectively protected from being invaded by pests; in addition, the repellent disclosed by the invention can also play a role in preventing and controlling various fruit fly pests at the same time, and has important significance on comprehensive management of pests of various crops. Therefore, when the phenylacetaldehyde is used for repelling pests, the use amount of chemical pesticides can be reduced, the pollution to the ecological environment is reduced, the requirements of modern green agriculture and sustainable development are met, and the phenylacetaldehyde has a good application value in pest repelling, preventing and controlling of bactrocera cucurbitae or South Asia fruit flies.

Description

Technical Field

[0001] The present invention relates to the field of pesticide formulations, and specifically relates to a melon fly and Bactrocera tau (Walker) repellent and its application. Background Art

[0002] The melon fly (Bactrocera cucurbitae (Coquillett)) and Bactrocera tau (Walker) are two important agricultural pests, which are characterized by a wide host range, strong reproductive ability, and serious damage. Their damage mode is mainly that female insects lay eggs in fruits, and the hatched larvae feed on the pulp, resulting in fruit rot, softening, and dropping, seriously affecting the yield and quality of fruits, and seriously threatening the livelihood of farmers and the national agricultural economy.

[0003] At present, the control methods for the melon fly and Bactrocera tau (Walker) mainly include chemical control, physical control, agricultural control, quarantine, biological control, etc. Among them, chemical control is the main pest control method at present due to its significant control effect, and the population of pests is reduced by spraying various chemical agents. However, there are still some problems with the current control strategies for the melon fly and Bactrocera tau (Walker). Chemical control is likely to cause pests to develop drug resistance, increasing the difficulty of control, and polluting the environment, affecting the survival of non-target organisms. The cost of bagging and setting up traps in physical control is relatively high, consuming a large amount of labor, and not suitable for large-scale application. Although agricultural control measures have a certain effect, they cannot completely eliminate pest damage, and there are many limitations in actual operation.

[0004] A repellent, also known as an insect repellent, is an active chemical substance produced by plants or artificially synthesized that has the effect of repelling insects. Different from chemical insecticides, a repellent can relieve the target being damaged by pests or provide early protection for potential targets that may be damaged by reducing or avoiding contact between pests and the target. However, the development of pest repellents is still in its infancy, with few known effective pest repellent components and high effective concentrations. The research and development of new pest repellents can provide new solutions for pest control.

[0005] Phenylacetaldehyde is an important organic compound with the molecular formula C8H8O and the structural formula A colorless to pale yellow liquid or solid with a unique aroma of hyacinth or almond fragrance. Phenylacetaldehyde widely exists in various plants such as roses, tomatoes, cucumbers, tobacco, zinnia, citrus fruits and honey, and is an important component of plant volatile aromas. Phenylacetaldehyde has a wide range of applications in many fields such as spices, food, chemical industry, agriculture, medicine and scientific research. Existing studies have found that phenylacetaldehyde has a dual role in insect behavior, which can either attract insects to perform behaviors such as foraging and oviposition, or show a repellent effect on certain insects. Its specific effect depends on the insect species, environmental conditions and the usage method of phenylacetaldehyde. However, there are few reports on the repellent effect of phenylacetaldehyde on Bactrocera cucurbitae and Bactrocera tau. Therefore, future research needs to further explore the chemical signal mechanism of phenylacetaldehyde and its optimized application strategy in pest management.

[0006] Therefore, there is an urgent need to develop a new type of control method that is efficient, environmentally friendly, friendly to the environmental ecology and non-target organisms, and conducive to the development of sustainable agriculture for Bactrocera cucurbitae and Bactrocera tau. Summary of the Invention

[0007] In view of the deficiencies of the prior art, the present invention provides a repellent for Bactrocera cucurbitae and Bactrocera tau and its application. The repellent of the present invention can be developed and utilized as a repellent by utilizing the repellent effect of specific odor volatiles on pests, so as to effectively control the two kinds of fruit flies.

[0008] In the present invention, the main active ingredient phenylacetaldehyde of the repellent is extracted from the volatiles of the host plant zucchini damaged by Bactrocera cucurbitae and Bactrocera tau, and it has been experimentally verified that it has a repellent effect on Bactrocera cucurbitae and Bactrocera tau at different concentrations.

[0009] To achieve the above objectives, the present invention is realized through the following technical solutions:

[0010] In the first aspect of the present invention, a repellent for Bactrocera cucurbitae and Bactrocera tau is provided, and the repellent is a preparation with phenylacetaldehyde as the active ingredient.

[0011] Further, the solvent of phenylacetaldehyde in the repellent is ultrapure water or dimethyl sulfoxide.

[0012] Furthermore, by weight of the components, the content of phenylacetaldehyde in the repellent is 0.005 - 0.2%.

[0013] Further, the repellent is made into a wet powder, aqueous solution, suspension, emulsion, aerosol, or sustained-release agent.

[0014] In the second aspect of the present invention, the application of the above repellent in the repellent control of Bactrocera cucurbitae or Bactrocera tau insects is provided.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0016] 1. High - efficiency repellent effect: As an effective repellent, phenylacetaldehyde in the present invention can significantly reduce the oviposition behavior of these two species of fruit flies on host plants, thus effectively protecting fruits from pest damage.

[0017] 2. Concentration - dependence: Different concentrations of phenylacetaldehyde in the present invention have different repellent effects on Bactrocera cucurbitae and Bactrocera tau. The repellent effect of phenylacetaldehyde shows a certain concentration - dependence. This provides a basis for selecting appropriate concentrations according to different requirements and scenarios in practical applications, which can not only ensure good repellent effects but also avoid unnecessary waste of resources.

[0018] 3. Broad - spectrum repellent effect on two species of fruit flies: The volatile phenylacetaldehyde odor of the present invention not only has a good repellent effect on Bactrocera cucurbitae but also is effective against Bactrocera tau. This shows that it has a wide range of applications and can play a prevention and control role against multiple species of fruit fly pests simultaneously, which is of great significance for the integrated pest management of multiple crops.

[0019] 4. Environment - friendly: Compared with traditional chemical pesticides, phenylacetaldehyde, as a naturally occurring compound, has lower environmental risks and toxicity. Using phenylacetaldehyde for pest repellency can reduce the usage amount of chemical pesticides and lower the pollution to the ecological environment, meeting the requirements of modern green agriculture and sustainable development. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the device for the two - choice test method in Example 1 of the present invention.

[0021] Figure 2 It is a graph showing the behavioral response results of female adult Bactrocera cucurbitae to different concentrations of phenylacetaldehyde in Example 2 of the present invention.

[0022] Figure 3 It is a graph showing the behavioral response results of female adult Bactrocera tau to different concentrations of phenylacetaldehyde in Example 2 of the present invention. DETAILED DESCRIPTION OF THE INVENTION

[0023] To better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention. The following describes the principles and features of the present invention. The examples given are only used to explain the present invention and are not intended to limit the scope of the present invention. In the description of the present invention, it should be noted that for those not specified in the embodiments, the conventional conditions or the conditions recommended by the manufacturer are followed. For reagents or instruments not specified by the manufacturer, they are all conventional products that can be obtained through commercial purchase.

[0024] Example 1. Two - choice test method

[0025] This example is used to provide a two-way selection test method of the present invention. In the present invention, the two-way selection test method is used to verify the repellent effects of the repellent of the present invention on female adults of Bactrocera cucurbitae and Bactrocera tau respectively. Figure 1 It is a two-way selection test device of the present invention, as Figure 1 shown. The test device of the present invention is an insect rearing cage of 60×60×60 cm. Inside the insect rearing cage, there are two zucchini slices about 1.5 cm long and about 4 cm in diameter, which are respectively used for smearing the compound to be tested and ultrapure water. The zucchini slice smeared with the compound to be tested and the control zucchini slice with ultrapure water are respectively placed at both ends of a foam box inside the insect rearing cage, and the distance between the two zucchini slices is 20 cm.

[0026] 50 female adults of Bactrocera cucurbitae or Bactrocera tau that have emerged for 18 - 20 d and are in the peak oviposition period are introduced into the insect rearing cage. 500 μL of the compound to be tested and 500 μL of ultrapure water are evenly smeared on the zucchini slices respectively. After 10 min, the results are observed, the number of female insects on the two zucchini slices is recorded respectively, and the repellent rates of the compound to be tested on female adults of Bactrocera cucurbitae and Bactrocera tau are calculated. Among them, the calculation method of the repellent rate is as follows:

[0027] Repellent rate (%) = [(number of female insects in the control group - number of female insects in the treatment group) / (number of female insects in the control group + number of female insects in the treatment group)] × 100%.

[0028] Example 2, Repellent effect of phenylacetaldehyde on Bactrocera cucurbitae and Bactrocera tau

[0029] This example is used to explore the repellent effect of phenylacetaldehyde on female adults of Bactrocera cucurbitae and Bactrocera tau. In this example, the main active ingredient of the repellent, phenylacetaldehyde, is extracted from the volatiles of the host plant zucchini damaged by Bactrocera cucurbitae and Bactrocera tau, and it has been verified to have a significant repellent effect on Bactrocera cucurbitae and Bactrocera tau through the following experiments.

[0030] The experimental method is as follows. Using ultrapure water as the solvent and control reagent of phenylacetaldehyde, phenylacetaldehyde reagents with three concentrations of 0.01 mg / mL, 0.1 mg / mL, and 1 mg / mL are respectively prepared as treatment groups with different concentrations. As an alternative implementation method, in this example, ultrapure water can be replaced by dimethyl sulfoxide (DMSO). Each concentration of the three concentrations is repeated 4 times. Using the test method described in Example 1, the repellent effects of phenylacetaldehyde on Bactrocera cucurbitae and Bactrocera tau are respectively tested, and the results are shown in Table 1, Figure 2 and Figure 3 shown. Among them, Table 1 is the table of the repellent effects of phenylacetaldehyde with different concentrations on female adults of Bactrocera cucurbitae and Bactrocera tau. Figure 2 and Figure 3 are respectively the graphs of the behavioral response results of female adults of Bactrocera cucurbitae and female adults of Bactrocera tau to phenylacetaldehyde with different concentrations.

[0031] Table 1 Repellent effects of phenylacetaldehyde at different concentrations on female adults of Bactrocera cucurbitae and Bactrocera tau

[0032]

[0033] The results show that the phenylacetaldehyde odor volatiles of the present invention have significant repellent effects on both Bactrocera cucurbitae and Bactrocera tau: when the concentration of phenylacetaldehyde is 0.1 mg / mL, the repellent rate against Bactrocera cucurbitae is 57.58% (as shown in Table 1 and Figure 2 shown, P = 0.049 < 0.05); when the concentration of phenylacetaldehyde is 1 mg / mL, the repellent rate against Bactrocera cucurbitae is 67.16%, and the repellent rate against Bactrocera tau is 64.86% (as shown in Table 1 and Figures 2 to 3 shown, for Bactrocera cucurbitae: P = 0.015 < 0.05; for Bactrocera tau: P = 0.019 < 0.05). Therefore, it can be known that different concentrations of phenylacetaldehyde in the present invention have different repellent effects on Bactrocera cucurbitae and Bactrocera tau, and the repellent effect of phenylacetaldehyde has a certain concentration dependence, which provides a basis for selecting appropriate concentrations according to different requirements and scenarios in practical applications. In addition, the phenylacetaldehyde odor volatiles of the present invention have good repellent effects on both Bactrocera cucurbitae and Bactrocera tau, indicating that it has a wide range of applications and can play a control role against multiple fruit fly pests at the same time, which is of great significance for the integrated pest management of multiple crops.

[0034] In addition, in the present invention, as a specific embodiment, the repellent agent with phenylacetaldehyde as the main active ingredient of the present invention can be made into wettable powder, aqueous solution, suspension, emulsion, aerosol, or sustained-release agent, etc. according to actual needs.

[0035] To sum up, the present invention uses a behavioral assay method to compare the behavioral responses of female adults of Bactrocera cucurbitae and Bactrocera tau to different concentrations of phenylacetaldehyde and finds that at different concentrations, the response degrees of female adults of Bactrocera cucurbitae and Bactrocera tau to it are different, which may be related to the physiological structure of the olfactory receptors of Bactrocera cucurbitae and Bactrocera tau and their sensitivity to odors. In addition, the present invention proves the repellent effect of phenylacetaldehyde on Bactrocera cucurbitae and Bactrocera tau, which can provide theoretical and technical support for the preparation of plant-derived repellents for Bactrocera cucurbitae and Bactrocera tau. The present invention also provides an application of a plant-derived repellent for female flies of Bactrocera cucurbitae and Bactrocera tau, laying a foundation for the green control of Bactrocera cucurbitae and Bactrocera tau.

[0036] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A repellent for Bactrocera cucurbitae and Bactrocera tau, characterized in that, The repellent is a preparation with phenylacetaldehyde as the active ingredient.

2. The melon fly and Bactrocera tau fruit fly repellent according to claim 1, characterized in that, The solvent of phenylacetaldehyde in the repellent is ultrapure water or dimethyl sulfoxide.

3. The melon fly and Bactrocera tau (Walker) repellent according to claim 2, characterized in that, By weight of the components, the content of phenylacetaldehyde in the repellent is 0.005 - 0.2%.

4. The melon fly and Bactrocera tau (Walker) repellent according to any one of claims 1 to 3, characterized in that, The repellent is made into wettable powder, aqueous solution, suspension, emulsion, aerosol, or sustained-release agent.

5. Application of the repellent according to any one of claims 1 - 4 in repelling and controlling pests such as Bactrocera cucurbitae or Bactrocera tau.

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