Cosmetic composition with multiple repairing effects, cosmetic and application

Abstract

The invention relates to the technical field of daily chemicals, in particular to a cosmetic composition with multiple repairing effects, a cosmetic and application. The cosmetic composition is prepared from a radish seed extract, glycolipid, phytosphingosine and a centella asiatica extract. The composition is further added with plant source active ingredients such as centella asiatica extract, radish seed extract and glycolipid, has good anti-inflammatory and antioxidant activity, and can effectively inhibit release of inflammatory factors, relieve uncomfortable reactions such as erythema and itching of skin and improve tolerance of sensitive skin. The lipid sealing agent, such as cetyl ethyl hexanoate, octyldodecanol and the like, has good compatibility with stratum corneum natural lipid, can form a sebum-like layer on the skin surface, assists in constructing a bionic'brick wall structure ', improves the moisture retention ability, reduces transdermal moisture loss (TEWL), and realizes long-acting moisture retention and environmental protection.

Description

Technical Field

[0001] The present invention relates to the field of daily chemical technology, and particularly relates to a cosmetic composition, a cosmetic, and an application having multiple repair effects. Background Art

[0002] The skin is the largest organ of the human body. It is not only the first barrier to protect the internal environment but also the primary contact interface for various external physical, chemical, and biological stimuli. Under the long-term or continuous action of various external factors (such as ultraviolet radiation, pollutants, over-cleaning, and bad living habits) and endogenous factors (such as emotional stress, hormonal fluctuations, and immune abnormalities), the skin is prone to a series of problems such as impaired barrier function, water loss, microecological imbalance, and enhanced inflammatory response, manifested as phenomena such as dryness, roughness, flushing, stinging, desquamation, and increased sensitivity, and may even evolve into a chronic inflammatory skin state.

[0003] Currently, there are many types of cosmetics for skin repair on the market, but most products only start from a single aspect. For example, moisturizing products mainly rely on humectants such as polyols or hyaluronic acid to achieve short-term water replenishment, anti-allergy products mostly achieve soothing effects by adding plant extracts such as glycyrrhizinate, purslane, and chamomile, while barrier repair products focus on adding structural lipids such as ceramides, cholesterol, and fatty acids to construct a biomimetic lipid membrane. However, the intervention method of a single component or a single path often has difficulty dealing with complex and multi-source skin barrier damage problems, and some active ingredients have poor stability or skin feel, resulting in limited actual use effects.

[0004] Therefore, there is an urgent need to develop a cosmetic composition with a multi-path and multi-level repair mechanism that can simultaneously meet the following requirements:

[0005] (1) Quickly soothe the skin and reduce the feeling of irritation;

[0006] (2) Promote the reconstruction of the skin barrier structure and enhance the water-locking ability;

[0007] (3) Activate the endogenous repair pathway of the skin and enhance its own defense and tolerance;

[0008] (4) Have good compatibility, skin feel, and stability, and are suitable for daily care or postoperative adjuvant repair of different skin types, especially sensitive skin and weak-barrier skin.

[0009] At the same time, among some skin-repairing cosmetics on the market, prohibited ingredients may be illegally added, such as glucocorticoids. Although these ingredients may bring significant repair effects in the short term, long-term use will cause damage to the skin and even lead to dependence. Many skin-repairing skin care products contain star ingredients but lack comprehensive ingredients and cannot achieve multiple repair effects. This results in the products being unable to effectively solve skin problems and may even damage the skin barrier due to the added high-concentration irritating ingredients. Summary of the Invention

[0010] To solve the above technical problems, the present invention provides a cosmetic composition with multiple repair effects, and the cosmetic composition includes: radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract.

[0011] As an embodiment of the present invention, the mass ratio of the radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract is: 0.02-0.2: 0.1-1: 0.05-0.3: 0.05-0.25.

[0012] As an embodiment of the present invention, the mass ratio of the radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract is: 0.02: 0.1: 0.05: 0.05.

[0013] As an embodiment of the present invention, the glycolipid is selected from: rhamnolipid, glucolipid, olive oil fermented glycolipid or a mixture thereof.

[0014] As an embodiment of the present invention, the cosmetic composition with multiple repair effects is applied to the daily chemical field.

[0015] The present invention provides a cosmetic with multiple repair effects, and the preparation raw materials of the cosmetic include the above-mentioned cosmetic composition with multiple repair effects.

[0016] As an embodiment of the present invention, the preparation raw materials of the cosmetic include: phytosphingosine, octyldodecanol, butyl octyl salicylate, cetyl ethylhexanoate, glycolipid, 305, water, acryloyldimethyltaurate / VP copolymer, disodium EDTA, pentylene glycol, hexylene glycol, centella asiatica extract, butylene glycol, radish seed extract.

[0017] As an embodiment of the present invention, the mass ratio of the cetyl ethylhexanoate and acryloyldimethyltaurate / VP copolymer is 4:1.

[0018] As an embodiment of the present invention, the cosmetic is in the form of an oil-in-water emulsion, gel milk, jelly cream or mask.

[0019] As an embodiment of the present invention, the method for preparing the cosmetic comprises the following steps:

[0020] (1) Aqueous phase pretreatment

[0021] Weigh deionized water and heat it to room temperature or slightly raise the temperature (about 35 °C).

[0022] Add successively: EDTA-2Na (fully dissolved), butylene glycol, pentylene glycol, hexylene glycol (stir and disperse), add acryloyldimethyltaurate / sodium acryloyldimethyltaurate copolymer (305): stir for 30 - 45 min until completely swollen to form a colloid, then add glycolipid (soluble or dispersed); add centella asiatica extract and radish seed extract, and stir evenly.

[0023] (2) Oil phase pretreatment

[0024] Weigh the oil phase components: octyldodecanol, cetyl ethylhexanoate, butyl octyl salicylate, slightly heat to 60 - 70 °C (to assist fusion), stir evenly and then add phytosphingosine.

[0025] (1) Slowly add the oil phase to the aqueous phase, stir evenly (homogenizer head at 2000 rpm), stir for about 10 - 15 min, and observe whether the emulsification is uniform; cool down to below 35 °C.

[0026] Adopting the above technical solution, the present invention has the following beneficial effects:

[0027] Raphanus sativus var. raphanistroides contains raforin, and some studies have proved that it has certain anti-cancer properties; 3-butenyl isothiocyanate has anti-mutagenic, chemopreventive, anti-fungal, antibacterial and antioxidant activities, and sulforaphane exhibits various properties in cell culture systems and animal models, including antioxidant, anti-inflammatory, anti-angiogenic and neuroprotective activities; sulforaphane and 3-butenyl isothiocyanate are known inducers of detoxification pathways and are adaptive responses to environmental stressors.

[0028] The radish seed extract is derived from organic Raphanus sativus var. raphanistroides sprouts, formed by sonication degradation method and liposome encapsulation, rich in 3-butenyl isothiocyanate, raforin, sulforaphane, flavonoids, various amino acids and vitamins, and can protect the DNA of skin cells epigenetically, stimulate the self-repair of DNA, reduce skin inflammatory reactions and delay the effect of photoaging.

[0029] Glycolipid has a unique structure and is difficult to prepare by chemical synthesis. This glycolipid can form a lamellar liquid crystal structure just by contacting with water, can quickly penetrate into the stratum corneum, repair the damaged lamellar liquid crystal structure in the stratum corneum, and can also increase the content of ceramide in epidermal cells, and can improve the epidermal barrier function of the skin and enhance the water retention capacity of the skin.

[0030] Phytosphingosine is known as the soft gold of plants and is a naturally occurring skin care ingredient. As a precursor of ceramide, phytosphingosine is relatively similar to skin lipids and is an important component of moisture retention and barrier function. It can promote ceramide synthesis and delay skin aging by promoting skin generation and collagen synthesis; effectively reduce the skin inflammation, erythema and irritation caused by UV radiation, and strengthen skin immune function; effectively inhibit the growth of Propionibacterium acnes and Staphylococcus aureus; it is also a natural inflammation regulator that inhibits various inflammatory factors (interleukin 1a and PKC).

[0031] The composition further adds plant-derived active ingredients such as centella asiatica extract, radish seed extract, and glycolipid, which have good anti-inflammatory and antioxidant activities, can effectively inhibit the release of inflammatory factors, relieve skin discomfort reactions such as erythema and itching, and improve the tolerance of sensitive skin. The lipid sealants such as cetyl ethylhexanoate and octyldodecanol have good compatibility with the natural lipids of the stratum corneum, can form a sebum-like layer on the skin surface, assist in constructing a bionic "brick wall structure", improve the moisture retention ability, reduce transepidermal water loss (TEWL), and achieve long-term moisturization and environmental protection. Brief Description of the Drawings

[0032] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. Obviously, the drawings in the following description are some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0033] Figure 1 : Before the facial flushing images of some volunteers in Example 3.

[0034] Figure 2 : After the facial flushing images of some volunteers in Example 3. Detailed Description of the Invention

[0035] The present invention provides a cosmetic composition with multiple repair effects. The cosmetic composition includes: radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract. As an embodiment of the present invention, the mass ratio of the radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract is: 0.02 - 0.2: 0.1 - 1: 0.05 - 0.3: 0.05 - 0.25. As a preferred embodiment of the present invention, the mass ratio of the radish seed extract, glycolipid, phytosphingosine, and centella asiatica extract is: 0.02: 0.1: 0.05: 0.05.

[0036] Radish seed extract

[0037] In the present invention, the preparation method of the radish seed extract is as follows:

[0038] (2) Select whole-fat radish seed powder and sieve it through 80 meshes. Then, add a composite plant enzyme to purified water to obtain a 0.3 wt% treatment solution. Subsequently, mix it at a material-liquid ratio of 1:12, and carry out enzymatic hydrolysis treatment at 45 °C and pH 6.5 for 90 min. Then, inactivate the enzyme at 80 °C for 10 min and cool for standby.

[0039] (3) In the same volume of culture medium as in step (1) (5 wt% of composite strains have been inoculated into the culture medium), ferment at 37 °C under mild micro-aerobic conditions for 48 h, with slight oscillation at 120 rpm during the period. Then, cool and filter through a 0.22 μm filter to remove bacteria.

[0040] Among them, the components of the culture medium include:

[0041] Glucose 1.0 wt%, yeast hydrolyzate 0.5 wt%, oat powder 0.5 wt%, protein-free soy hydrolyzate 0.3 wt%, glycerol / sorbitol 0.5 wt%, trehalose 0.2 wt%, sodium citrate 0.05 wt%, sodium phosphate 0.05 wt%, and water is supplemented to 100%.

[0042] Among them, the composite strains are: Lactobacillus plantarum (2 wt%), Bacillus subtilis (2 wt%), yeast (1 wt%).

[0043] The present invention provides a cosmetic with multiple repair effects. The preparation raw materials of the cosmetic include: phytosphingosine, octyldodecanol, butyloctyl salicylate, cetyl ethylhexanoate, glycolipid, 305, water, acryloyldimethyltaurate / VP copolymer, disodium EDTA, pentylene glycol, hexylene glycol, centella asiatica extract, butanediol, radish seed extract.

[0044] As an embodiment of the present invention, the preparation raw materials of the cosmetic include: phytosphingosine 0.05 - 0.3 parts, octyldodecanol 2 parts, butyloctyl salicylate 2 parts, cetyl ethylhexanoate 2 parts, glycolipid 0.1 - 0.5 parts, 305 0.5 parts, acryloyldimethyltaurate / VP copolymer 0.5 parts, disodium EDTA 0.05 parts, pentylene glycol 2 parts, hexylene glycol 0.5 parts, centella asiatica extract 0.05 - 0.25 parts, butanediol 5 parts, radish seed extract 0.02 - 0.2 parts, and water is supplemented to 100 parts.

[0045] The preparation method of the cosmetic is as follows:

[0046] (1) Aqueous phase pretreatment

[0047] Weigh deionized water and heat it to room temperature or slightly raise the temperature (about 35 °C)

[0048] Add in sequence: EDTA-2Na (fully dissolved), butylene glycol, pentylene glycol, hexylene glycol (stir and disperse), add acryloyldimethyltaurate / sodium vinylsulfonate copolymer (305): stir for 30 - 45 min until completely swollen to form a colloid, then add glycolipid (soluble or dispersible); add Centella asiatica extract and Raphanus sativus seed extract, and stir evenly.

[0049] (2) Oil phase pretreatment

[0050] Weigh the oil phase components: octyldodecanol, cetyl ethylhexanoate, butyl octyl salicylate, slightly heat to 60 - 70 °C (to assist fusion), stir evenly and then add phytosphingosine.

[0051] (4) Slowly add the oil phase to the water phase, stir evenly (homogenizer at 2000 rpm), stir for about 10 - 15 min, and observe whether the emulsification is uniform; cool down to below 35 °C.

[0052] In conventional repair products, to improve the repair effect, ceramide liposomes and jojoba oil are directly added. However, in this system, ceramide liposomes are a particulate colloid system and are easily incompatible with high-molecular colloids (such as 305), resulting in turbidity and precipitation. At the same time, jojoba oil belongs to esters with medium molecular weight. After adding, the stickiness of the product is significantly improved, and the transparency of the product also decreases because the water-in-oil ratio is disrupted, and the emulsification performance becomes unstable. After long-term storage of the product, layering, water separation, etc. will occur. Often, to avoid the above situation, PEG-100 stearate is added to the formula, but it will make the product greasy. However, phytosphingosine is sensitive to the oil phase and is prone to crystallization.

[0053] Therefore, the present invention breaks the conventional formula, and the compound of Raphanus sativus seed extract, glycolipid, and phytosphingosine can achieve the product effect of adding ceramide liposomes and jojoba oil; at the same time, the product is overall refreshing and stable. However, during the product preparation process, glycolipid is not easily completely compatible with the water phase or alcohols, and there is a risk of demulsification.

[0054] However, since the present invention does not directly add ceramide to the formula but uses phytosphingosine as a precursor of ceramide, its effect will be slightly weaker than directly adding ceramide. Therefore, when preparing Raphanus sativus seed extract in the present invention, a "probiotic + postbiotic co-metabolism" environment is constructed to enhance the stable output of metabolites such as short-chain fatty acids, soothing polypeptides, lactic acid, etc., and improve the deep conditioning ability of the stratum corneum barrier.

[0055] The following further explains and illustrates the present invention in combination with specific embodiments.

[0056] The formula is illustrated by the examples according to the formula shown in Table 1.

[0057] As described in the formula of Table 1

[0058]

[0059]

[0060] The preparation methods of the above Examples 1 to 3 are as follows:

[0061] (1) Aqueous phase pretreatment

[0062] Weigh deionized water and heat it to room temperature or slightly higher (about 35°C).

[0063] Add successively: EDTA-2Na (fully dissolved), butylene glycol, pentylene glycol, hexylene glycol (stir and disperse), add the copolymer of hydroxyethyl acrylate / sodium taurate (305): stir for 30–45 min until completely swollen to form a colloid, then add glycolipid (soluble or dispersed); add centella asiatica extract and radish seed extract, and stir evenly;

[0064] (2) Oil phase pretreatment

[0065] Weigh the oil phase components: octyldodecanol, cetyl ethylhexanoate, butyloctyl salicylate, slightly heat to 60-70°C (to assist fusion), stir evenly and then add phytosphingosine;

[0066] (3) Slowly add the oil phase to the aqueous phase, stir evenly (homogenizer head at 2000 rpm), stir for about 10-15 min, and observe whether the emulsification is uniform; cool to below 35°C.

[0067] The preparation method of the said radish seed extract is as follows:

[0068] (1) Select whole-fat radish seed powder and sieve it through 80 mesh, then add composite plant enzymes (bromelain, chitosanase, β-glucosidase) to purified water to obtain a 0.3 wt% treatment solution; then mix according to a solid-liquid ratio of 1:12, and carry out enzymatic hydrolysis treatment at 45°C and pH 6.5 for 90 min, then heat inactivate the enzyme at 80°C for 10 min, and cool for standby;

[0069] (2) In the same volume of culture medium as in step (1) (5 wt% composite bacteria have been inoculated into the culture medium), ferment at 37°C under mild microaerobic conditions for 48 h, with slight oscillation at 120 rpm during the period; then cool and filter through a 0.22 μm filter to remove bacteria.

[0070] Among them, the components of the culture medium include:

[0071] Glucose 1.0 wt%, yeast hydrolysate 0.5 wt%, oat powder 0.5 wt%, protein-free soy hydrolysate 0.3 wt%, glycerol / sorbitol 0.5 wt%, trehalose 0.2 wt%, sodium citrate 0.05 wt%, sodium phosphate 0.05 wt%, water added to 100%.

[0072] Among them, the composite strains are: Lactiplantibacillus plantarum (2 wt%), Bacillus subtilis (2 wt%), Saccharomyces cerevisiae (1 wt%).

[0073] Lactiplantibacillus plantarum: Lactiplantibacillus plantarum 25125.

[0074] Bacillus subtilis: Bacillus subtilis 10732.

[0075] Saccharomyces cerevisiae: Rhodosporidium toruloides 32489.

[0076] The preparation methods of the above Comparative Examples 1 to 7 refer to Examples 1 to 3, in which ceramide liposomes and collagen are both prepared in the aqueous phase.

[0077] Performance testing

[0078] I. Testing instruments:

[0079] 1. The present invention uses the Finnish Delfin VapoMeter SWL5201 transdermal water loss measuring instrument. The VapoMeter uses the principle of measuring in a closed chamber, so the measurement is not affected by indoor air currents. The measurement time is short, ensuring the minimization of blocking normal evaporation.

[0080] 2. Use the Finnish Delfin Antera 3D for taking pictures. The Antera 3D uses innovative optical methods and complex mathematical algorithms to obtain three-dimensional images. In this way, data can be extracted from the images related to the three-dimensional shape of the skin, which will be able to quantify the treatment effect and monitor the changes over time. For example, measure the texture, smoothness of the skin, and the depth and width of fine lines and wrinkles. With its multispectral analysis, the Antera software can also measure pigmentation and redness, which will provide meaningful information about sun damage, veins, etc.

[0081] II. Experimental principle:

[0082] The TEWL value, Transepidermal water loss (TEWL), measures the change in the water vapor pressure adjacent to the skin surface. The TEWL value represents the water loss of the stratum corneum and is an important criterion for evaluating the barrier function of the stratum corneum. The lower the TEWL value of the skin, the better the barrier function of the skin, and vice versa. By testing the TEWL value of skin water loss, the efficacy of moisturizing and repairing cosmetics can be evaluated, and it can also be applied to allergic patch tests, contact dermatitis, physical therapy, burn and new tissue monitoring to timely detect whether the protective function of the skin has been damaged. The unit of TEWL is: g / hm 3 .

[0083] III. Test population:

[0084] There are 25 people in each group, with a total of 8 groups, and the volunteers aged 18 - 60 years meet the experimental requirements.

[0085] IV. Test conditions:

[0086] At room temperature of 20 ± 2°C and relative humidity of 50 ± 10%, and with real-time dynamic monitoring, the subjects sit still for 30 minutes and then relevant data is tested.

[0087] V. Experimental process:

[0088] This experiment is divided into 8 groups. The testers in the 1st - 4th groups apply Example 1 on the left face and Comparative Examples 1 - 4 on the right face respectively; the subjects in the 5th - 7th groups apply Example 3 on the left face and Comparative Examples 5 - 7 on the right face; the subjects in the 8th group apply Example 2 on the left face and Comparative Example 8 on the right face. It is applied to the face twice a day, morning and evening, for 4 consecutive weeks, and their TEWL values are recorded. Then, for each volunteer, one part is selected from the left and right cheeks of the face for Antera3D photography, and the average RED value of skin flushing is recorded and archived.

[0089] VI. Experimental results:

[0090] Measurement of the transcutaneous water loss rate (TEWL) and the average skin flushing value (RED) of the skin

[0091] In this test, the TEWL measured at the 0th week of the subjects is set as TEWL0, the TEWL at the 4th week is set as TEWL4, the change value of TEWL is △TEWL, △TEWL = TEWL4 - TEWL0. If △TEWL is negative and the value is lower, it indicates that the less water is lost from the skin after four weeks, that is, the better the skin barrier function is repaired and the healthier the skin is;

[0092] The RED setting for the 0th week is RED0, and the RED setting for the 4th week is RED4. The change value of RED is △RED, and △RED = RED4 - RED0. △RED is a negative number, and the lower the value, the less the skin is flushed, that is, the less the inflammatory reaction of the basal skin, and the more comfortable the skin is, indicating that the soothing and repair effect of the product is better. Table 2 shows the average △TEWL value and △RED value of 25 people in each group.

[0093]

[0094]

[0095] The experimental results show that:

[0096] It can be seen from Table 2 of this experimental result that:

[0097] For the △TEWL value and △RED value, Example 1 > Example 2 > Example 3. From this, it can be shown that as the addition ratio of the composition of the present invention increases, the △TEWL and △RED values will correspondingly become smaller, that is, the less the skin moisture loss, the less the skin flushing, the less likely the skin is to lose moisture, and the inflammatory condition of the muscle bottom is also alleviated, indicating that the skin barrier function and resistance are better.

[0098] The △TEWL and △RED values of Example 1 are less than those of Comparative Example 1, Comparative Example 2, Comparative Example 3, and Comparative Example 4, indicating that removing any one component from the composition of the present invention will make the corresponding △TEWL and △RED values increase, that is, removing any component will weaken the function of the composition of the present invention to repair the skin.

[0099] The △TEWL and △RED values of Example 3 are less than those of Comparative Example 5, indicating that increasing the addition amount of the composition of the present invention will not increase but decrease the repair effect of the product, and the increase in the component content will cause skin intolerance and increase the skin burden, and the effect is much worse than that within the range of the composition of the present invention.

[0100] The △TEWL and △RED values of Example 3 are less than those of Comparative Example 6 and Comparative Example 7, indicating that replacing the composition of the present invention with ceramide and collagen, which are more recognized for their repair effects on the market, will weaken their effects.

[0101] The △TEWL and △RED values of Example 2 are less than those of Comparative Example 8, indicating that the repair effect of the composition of the present invention is better than that of the commercial product.

[0102] Comparison of test pictures

[0103] Select a part of the volunteers' facial flushing images before and after using the examples of the present invention for comparison. For details, seeFigure 1 As can be visually seen from the figure, after 28 days of use by volunteers with severe facial flushing, the effect of the composition of the present invention in soothing and repairing facial flushing is very excellent.

[0104] Safety test (human skin patch test)

[0105] 1. Number of test subjects: A total of 40 people, 20 men and 20 women, aged between 18 - 30 years old.

[0106] 2. Health status: The skin of the subjects is healthy and has no history of skin diseases or allergies, meeting the volunteer inclusion criteria for the subjects.

[0107] 3. Test method: Select a qualified patch tester. In a closed patch test method, approximately 0.05 g of the test product is dropped into the patch tester and externally applied to the back of the subject with a special tape. There are 11 application points for each subject. After 24 hours, the test substance is removed. Observe the skin reaction at 0.5, 24, and 48 hours after removal respectively. Score according to the skin reaction grading standard in the "Hygiene Standard for Cosmetics", calculate the average value, and record the results in Table 4.

[0108] 4. Evaluation criteria are shown in Table 3

[0109] Table 3 Standard Table for Human Skin Patch Test

[0110]

[0111] Table 4 Scoring Results of Skin Patch Test

[0112]

[0113] As can be seen from Table 4, each component of the composition of the present invention is very safe, very friendly to the skin, and mild and non - irritating.

[0114] Comparative Example 9: The difference between Comparative Example 9 and Example 3 is that the radish seed extract is water - extracted.

[0115] Comparative Example 10: The difference between Comparative Example 10 and Example 3 is that the components of the fermentation medium in the radish seed extract are: glucose 1.0 wt%, yeast hydrolyzate 0.5 wt%, protein - free soy hydrolyzate 0.3 wt%, glycerol / sorbitol 0.5 wt%, trehalose 0.2 wt%, sodium citrate 0.05 wt%, sodium phosphate 0.05 wt%, and water is made up to 100%.

[0116] Comparative Example 11:: The difference between Comparative Example 11 and Example 3 is that the preparation method of the radish seed extract is as follows:

[0117] (1) Select full-fat radish seed powder and sieve it through 80 mesh. Then add bromelain to purified water to obtain a 0.3 wt% treatment solution. Subsequently, mix it at a solid-liquid ratio of 1:12, and carry out enzymatic hydrolysis at 45 °C and pH 6.5 for 90 min. Then, inactivate the enzyme at 80 °C for 10 min and cool for standby.

[0118] (2) In the same volume of culture medium as in step (1) (5 wt% Lactobacillus plantarum has been inoculated into the culture medium), ferment at 37 °C under mild microaerobic conditions for 48 h, with gentle oscillation at 120 rpm during this period. Then cool and filter through a 0.22 μm filter to remove bacteria.

[0119] Among them, the components of the culture medium include:

[0120] Glucose 1.0 wt%, yeast hydrolysate 0.5 wt%, oat powder 0.5 wt%, protein-free soy hydrolysate 0.3 wt%, glycerol / sorbitol 0.5 wt%, trehalose 0.2 wt%, sodium citrate 0.05 wt%, sodium phosphate 0.05 wt%, and water is supplemented to 100%.

[0121] Physicochemical property test

[0122] Place the skin care products prepared in Example 3 and Comparative Examples 9 - 11 at 0 °C, 25 °C, and 40 °C for 90 d, and check the product properties and colors. The test results are shown in Table 5.

[0123] Thermal cycling experiment: Place the skin care products prepared in Example 3 and Comparative Examples 9 - 11 into a sealed container to ensure it is completely sealed to prevent interference from external humidity or gas. Put the samples into an incubator set at 45 °C and keep for 12 hours. After taking out the samples, quickly transfer them to a low-temperature box at -5 °C and keep for 12 hours. Repeat 6 times after completing 1 high-low temperature cycle; check the physical properties such as the appearance, color, odor, viscosity, layering, and particle precipitation of the samples after cycling.

[0124] Table 5 Test results of physicochemical property test

[0125]

[0126]

[0127] At the same time, test the transepidermal water loss rate (TEWL) and average skin redness value (RED) of the skin in Comparative Examples 9 - 11 to obtain Table 6.

[0128] Table 6 Test results

[0129] Group Change rate of △TEWL (%) Change rate of △RED (%) Comparative Example 9 -13.6 -17.2 Comparative Example 10 -20.1 -22.8 Comparative Example 11 -22.9 -26.3

[0130] At the same time, check the appendix Figure 1, Before and after comparison of the facial flushing images of some volunteers using Example 3 of the present invention, see Figure 1 , It can be visually seen from the figure that after 28 days of use by volunteers with relatively severe facial flushing, the effect of the composition of the present invention in soothing and repairing facial flushing is very excellent.

[0131] Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A cosmetic composition with multiple repair effects, characterized in that, The cosmetic composition comprises: radish seed extract, glycolipid, phytosphingosine, and asiatic pennywort extract.

2. The cosmetic composition having multiple repair effects according to claim 1, wherein The mass ratio of radish seed extract, glycolipid, phytosphingosine, and asiatic pennywort extract is: 0.02 - 0.2: 0.1 - 1: 0.05 - 0.3: 0.05 - 0.

25.

3. The cosmetic composition with multiple repair effects according to claim 1, characterized in that, The mass ratio of radish seed extract, glycolipid, phytosphingosine, and asiatic pennywort extract is: 0.02: 0.1: 0.05: 0.

05.

4. The cosmetic composition with multiple repair effects according to claim 1, characterized in that, The glycolipid is selected from: rhamnolipid, glucolipid, olive oil fermented glycolipid or a mixture thereof.

5. The cosmetic composition with multiple repair effects according to claim 1, characterized in that, It is applied to the daily chemical field.

6. A cosmetic with multiple repair effects, characterized in that, The preparation raw materials of the cosmetic comprise the cosmetic composition with multiple repair effects according to any one of claims 1 - 5.

7. The cosmetic according to claim 6, characterized in that, The preparation raw materials of the cosmetic comprise: phytosphingosine, octyldodecanol, butyloctyl salicylate, cetyl ethylhexanoate, glycolipid, 305, water, hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer, disodium EDTA, pentylene glycol, hexylene glycol, asiatic pennywort extract, butylene glycol, radish seed extract.

8. The cosmetic according to claim 7, characterized in that, The mass ratio of cetyl ethylhexanoate and hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer is 4:

1.

9. The cosmetic according to claim 6, characterized in that, It is in the form of an oil-in-water emulsion, gel milk, jelly cream or mask.

10. A method for preparing a cosmetic according to claim 6, characterized in that, It includes the following steps: (1) Aqueous phase pretreatment Weigh deionized water and heat it to room temperature or slightly higher. Add successively: disodium EDTA - 2Na, butylene glycol, pentylene glycol, hexylene glycol, add hydroxyethyl acrylate / sodium taurate copolymer: stir for 30 - 45 min until completely swollen to form a colloid, then add glycolipid; add asiatic pennywort extract, radish seed extract, and stir evenly. (2) Oil phase pretreatment Weigh the oil phase components: octyldodecanol, cetyl ethylhexanoate, butyloctyl salicylate, slightly heat to 60 - 70 °C, stir evenly and then add phytosphingosine. (3) Slowly add the oil phase to the aqueous phase, stir evenly, stir for about 10 - 15 min, and observe whether the emulsification is uniform; cool down to below 35 °C.

Images