Bacteriostatic, anti-dandruff, oil-control, soothing and anti-allergy composition and application thereof

Abstract

The invention relates to a composition capable of inhibiting bacteria, removing dandruff, controlling oil, relieving and resisting allergy. The composition comprises a biological glycolipid surfactant, an astragalus membranacus extract and a yeast / zinc fermentation product, the components are combined according to the mass ratio of (1 to 3): (0.5 to 1.5): (0.05 to 0.10); wherein the astragalus membranacus extract is an aqueous extract of astragalus membranacus, and the relative density of the astragalus membranacus extract at 60 DEG C is 1.15-1.20; and the relative density of the yeast / zinc fermentation product at 20 DEG C is 1.18-1.22. The composition can wash off dirt generated by hair, is good in stability, can quickly kill malassezia and regulate grease secretion of scalp, and achieves the effects of relieving sebum, mildly removing dandruff, improving the scalp micro-ecological environment and repairing the natural barrier of the scalp (preventing the percutaneous water loss rate from increasing); the technical problems that in the prior art, scalp is injured after long-term use, the environmental protection property is poor, the effect is slow, and the flora proportion is difficult to effectively adjust are solved.

Description

Technical Field

[0001] The present invention belongs to the field of daily chemical products, and specifically relates to an antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition and its application. Background Art

[0002] At present, the addition of some chemical substances in many commercially available shampoos, including antibacterial agents, synthetic surfactants, lubricants and preservatives, will damage the scalp to varying degrees. The current scalp care field faces three major technical bottlenecks: (1) The contradiction between the efficacy and irritation of surfactants: Mainstream anti-dandruff products rely on sulfate surfactants (such as SLES / SLS). Although they have strong cleaning power (detergency > 95%), long-term use leads to damage to the scalp barrier (the trans-epidermal water loss rate TEWL increases by 25 - 40%). According to the research report in the "Journal of Dermatological Science", SLES will damage the lipid bilayer structure of the stratum corneum (the ceramide content is reduced by 18%), exacerbating scalp sensitivity. Although environmentally friendly glucoside surfactants (APGs) are gradually being applied, their oil-control ability is weak (the oil adsorption amount is only 60% of that of SLES), and they are prone to gelation at low temperatures (the viscosity increases steeply by 3 times below 25°C), restricting the formulation stability. (2) The ecological and efficacy limitations of chemical antibacterial agents: Traditional zinc salts (such as ZPT, OCT) are restricted from use by the new EU SCCS 2024 regulations due to environmental residue problems (the maximum concentration is reduced to 0.8%), and alternative ingredients such as OCT have a 50% reduction in antibacterial efficiency in a high pH environment (> 6.5). Natural antibacterial ingredients (such as tea tree oil) have high safety, but the killing time of Malassezia is poor (it needs to be in continuous contact for more than 6 hours). It is impossible for conventional shampoo to keep tea tree oil in contact with Malassezia for such a long time, and the compatibility of tea tree oil with anionic surfactants is poor (the layering rate reaches 30% due to pH conflict in the system). (3) The fragmentation of oil control and microecological regulation: Existing technologies mostly use physical adsorption (silica particles) or sebum secretion inhibitors (such as niacinamide), but the former is easy to clog hair follicles (the hair follicle deposition rate > 15%), and the latter takes 28 days of continuous use to take effect, with a very slow effect. The scalp microbiome research in 2024 reveals that both Propionibacterium and Staphylococcus play important roles in the scalp environment. Propionibacterium usually interacts with the sebum secreted by the sebaceous glands, helping to maintain the normal physiological state of the scalp. However, in some cases, such as excessive sebum secretion or clogged pores, it may promote the occurrence of acne. Staphylococcus epidermidis, usually considered one of the beneficial bacteria on the skin, can inhibit the growth of pathogens and help maintain the skin barrier function. The health of the scalp is closely related to the balance of the microbial community. When this balance is broken, it may lead to scalp problems such as dryness, itching, dandruff, and even more serious inflammatory diseases. However, existing technologies are difficult to effectively regulate the flora. Summary of the Invention

[0003] (1) Technical problems to be solved

[0004] In view of the above-mentioned disadvantages and deficiencies of the prior art, the present invention provides an antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition. The composition can not only wash away the dirt generated by the hair, has good stability, but also can quickly kill Malassezia and regulate the sebum secretion of the scalp, achieving the effect of soothing sebum and gently removing dandruff, improving the scalp microecological environment, and repairing the natural barrier of the scalp (preventing the increase of transepidermal water loss rate), thus solving the technical problems existing in the prior art such as long-term use causing scalp damage, poor environmental protection, slow effect, and difficulty in effectively regulating the flora ratio.

[0005] (2) Technical solutions

[0006] In a first aspect, the present invention provides an antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition, which comprises a biosurfactant, an extract of Astragalus membranaceus and a yeast / zinc ferment. They are combined in a mass ratio of 1-3: 0.5-1.5: 0.05-0.10. Among them, the extract of Astragalus membranaceus is an aqueous extract of Astragalus membranaceus, and its relative density at 60 °C is 1.15-1.20; the relative density of the yeast / zinc ferment at 20 °C is 1.18-1.22.

[0007] According to a preferred embodiment of the present invention, the biosurfactant, the extract of Astragalus membranaceus and the yeast / zinc ferment are combined in a mass ratio of 1: 1: 0.10; or in a mass ratio of 2: 1: 0.05; or in a mass ratio of 3: 1.5: 0.10.

[0008] According to a preferred embodiment of the present invention, the biosurfactant is sophorolipid and / or rhamnolipid.

[0009] According to a preferred embodiment of the present invention, the yeast / zinc ferment is obtained by adding inorganic zinc to the culture medium during the growth of yeast, using the absorption and transformation of zinc by yeast to obtain yeast rich in organic zinc, and then releasing the yeast intracellular substances through bioenzymolysis technology and collecting them to obtain the yeast / zinc ferment.

[0010] According to a preferred embodiment of the present invention, the sophorolipid is obtained by inoculating Candida apicola on glucose and vegetable oil and fermenting and separating and purifying under sterile conditions. Candida apicola is also known as Candida bombicola and Torulopsis apicola.

[0011] According to a preferred embodiment of the present invention, the preparation method of the rhamnolipid is: inoculating Pseudomonas microorganisms in a carbon source and vegetable oil, and fermenting and separating and purifying under sterile conditions. The Pseudomonas microorganisms can be Pseudomonas aeruginosa or Pseudomonas putida; the carbon source is at least one of glucose, glycerol and citric acid.

[0012] According to a preferred embodiment of the present invention, the glycolipid is sophorolipid or rhamnolipid, such as mature products selected from sophorolipid, rhamnolipid, or a mixture of sophorolipid and rhamnolipid produced and sold by Shanghai Bokoo Biotechnology Co., Ltd.

[0013] Preferably, for the sophorolipid raw material used in the present invention, the mass ratio of acid-type sophorolipid is 0-30%, and the mass ratio of lactone-type sophorolipid is 100-70%; the total of the two sophorolipids is 100%.

[0014] In a second aspect, the present invention also provides a hair washing and care product, which contains the composition of any one of the above embodiments.

[0015] Preferably, the hair washing and care product further includes one or more of a main surfactant, an auxiliary surfactant, a thickener, a co-solvent, a chelating agent, a preservative, a pearlescent agent, a conditioner, a buffer, a fragrance, and a pigment.

[0016] The main surfactant can be sodium laureth sulfate (AES) and / or coconut fatty acid monoethanolamide (CMEA); the chelating agent is EDTA-2Na; the pearlescent agent is ethylene glycol distearate; the conditioner is cocamidopropyl betaine, the preservative is 1,2-hexanediol, the buffer is citric acid, and the thickener is sodium chloride.

[0017] Coconut fatty acid monoethanolamide (CMEA) is a non-ionic surfactant and can also be used as a thickener and foam stabilizer. Among them, sodium laureth sulfate (AES) is an anionic surfactant, mainly responsible for decontamination, foaming, and cleaning; cocamidopropyl betaine (CAB-35) is an amphoteric surfactant with good cleaning performance, and at the same time can increase the viscosity of the system and improve the mildness of the product.

[0018] According to a preferred embodiment of the present invention, the hair washing and care product, by mass percentage, has the following composition: 2.0-3% of CMEA, 15.0-18% of AES, 0.01-0.03% of EDTA-2Na, 2-3% of the composition, 0.4-0.6% of ethylene glycol distearate, 4-6% of cocamidopropyl betaine, 0.4-0.6% of 1,2-hexanediol, 0.1-3% of citric acid, 0.8-1.2% of sodium chloride; the balance is water.

[0019] According to a preferred embodiment of the present invention, the hair washing and care product uses the antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition of any one of the above embodiments as the sole active ingredient.

[0020] (III) Beneficial effects

[0021] (1) The antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition of the present invention uses a biosurfactant, an Astragalus membranaceus extract, and a yeast / zinc ferment. Among them, the biosurfactant is milder than traditional surfactants such as SLS or SLES and is environmentally friendly; the Astragalus membranaceus extract has anti-inflammatory or antioxidant effects, can well soothe the scalp and reduce scalp sensitivity, and the yeast / zinc ferment may regulate the scalp microecology to achieve the purpose of oil control; the composition of the present invention has low irritation and is environmentally friendly. It can not only wash away the dirt generated by the hair, has stable efficacy, but also can quickly kill Malassezia and regulate scalp oil secretion, achieving the effect of soothing sebum and mild dandruff removal, improving the scalp microecological environment, and repairing the natural scalp barrier, solving the technical problems existing in the prior art such as long-term use causing scalp damage, poor environmental friendliness, slow effect, and difficulty in effectively regulating the flora ratio.

[0022] (2) The biosurfactant in the composition of the present invention itself has the functions of foaming, cleaning and antibacterial, and can also promote the uniform dispersion of the components in the Astragalus membranaceus extract and the yeast / zinc ferment, as well as promote the stability of the composition phase. It can also promote the penetration of the active ingredients in the Astragalus membranaceus extract and the yeast / zinc ferment into the scalp surface cells and hair follicles, improve the bioavailability, and cooperate with yeast / zinc to inhibit bacteria, control oil and regulate the flora structure.

[0023] (3) Experiments have proved that the composition of the present invention has the effect of soothing the scalp; the three components can play a synergistic role in the process of inhibiting Propionibacterium acnes and Malassezia furfur and removing the biofilms formed by these bacteria, play a synergistic role in the function of repairing scalp barrier damage and reducing the transepidermal water loss rate, have a synergistic promoting effect in shortening the killing time limit of Malassezia and the inhibition time limit of sebum secretion, and are not easy to block hair follicles, and have a synergistic promoting effect in restoring the balance of the scalp microbial community. Compared with natural tea tree oil antibacterial agents and nicotinamide sebum secretion inhibitors, etc., it has the advantages of faster antibacterial speed, short onset time, and remarkable effect. Therefore, it can be used to prepare shampoos and hair care products, and has the functions of mild antibacterial, anti-dandruff, soothing and repairing. Description of the Drawings

[0024] Figure 1 The removal effect of sample 10# of Example 3 on the biofilm of Malassezia furfur at different concentrations.

[0025] Figure 2 The removal effect of sample 11# of Example 3 on the biofilm of Malassezia furfur at different concentrations.

[0026] Figure 3 The inhibition curve of sample 10# of Example 4 on the activity of hyaluronidase. Detailed Embodiments

[0027] For better explaining the present invention for easy understanding, the present invention will be described in detail below in conjunction with the accompanying drawings through specific embodiments.

[0028] The present invention provides an antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition, which comprises a biosurfactant, an Astragalus membranaceus extract and a yeast / zinc ferment; they are combined in a mass ratio of 1-3:0.5-1.5:0.05-0.10; wherein, the Astragalus membranaceus extract is an aqueous extract of Astragalus membranaceus, and its relative density at 60 °C is 1.15-1.20; the relative density of the yeast / zinc ferment at 20 °C is 1.18-1.22. Among them, the biosurfactant is sophorolipid and / or rhamnolipid, and both sophorolipid and rhamnolipid are mature commercial products that have been commercially produced and can be purchased from the market. In the present invention, preferably, the mass proportion of acid-type sophorolipid in the sophorolipid raw material is 0-30%, and the mass proportion of lactone-type sophorolipid is 100-70%.

[0029] Sophorolipid can be prepared according to the following method:

[0030] S1. Mix glucose and vegetable oil in a mass ratio of 3:1-4:1. The vegetable oil is rapeseed oil and / or soybean oil, and the vegetable oil contains ≥60% of C18:1 fatty acid. Add sterile water to make the initial concentration of glucose 80-120 g / L and the initial concentration of vegetable oil 30-50 g / L; then, add 5-10 g / L of glycerol, 2-3 g / L of ammonium sulfate, 1-2 g / L of yeast extract powder, and 0-4 g / L of trace elements, and mix evenly to obtain a fermentation substrate;

[0031] S2. Transfer the fermentation substrate to a sterile fermenter, inoculate Candida apicola in the logarithmic growth phase with an OD 600 = 12-15 at 8-12% of the volume of the fermentation substrate. The initial pH of the fermentation is 6.0. During the fermentation process, the pH of the fermentation broth is maintained at 5.8-6.2 by adding ammonia water, the temperature is maintained at 30±0.5 °C, and the dissolved oxygen is controlled at 30%-50% saturation. During the 0-24 h of the fermentation process, the dissolved oxygen is controlled at 50% to promote the proliferation of bacteria. After 24 h of fermentation, the dissolved oxygen is controlled at 30-40% to induce the accumulation of metabolites; when the residual sugar drops to 30 g / L, glucose is added at a rate of 5-8 g / (L·h) until the total sugar concentration ≤200 g / L. After 48 h of fermentation, vegetable oil is added at one time to 10-15 g / L;

[0032] S3. When the fermentation reaches 96 h, or it is detected that the yield of sophorolipid no longer increases, or it is detected that the residual sugar <5 g / L and the oil conversion rate >85%, terminate the fermentation;

[0033] S4. After fermentation, centrifuge at 8000 - 10000×g for 10 - 20 min to remove the cells and untransformed oil. Adjust the pH of the supernatant to 2.0 - 2.5 with dilute hydrochloric acid, and let it stand at 4°C for 10 - 16 h to precipitate sophorolipid micelles. Centrifuge to collect the precipitate, and extract it 2 - 4 times by shaking with an ethyl acetate - water two - phase system with a v / v ratio of 1:1. Combine the organic phases and remove the solvent by vacuum distillation to obtain crude sophorolipid. Dissolve the crude product in hot ethanol at 55 - 65°C, cool it for recrystallization to obtain white needle - shaped crystals, and freeze - dry to obtain the sophorolipid product.

[0034] The trace elements in S1 include: 1.5 - 2.5 g / L of ammonium dihydrogen phosphate, 0.5 - 1.0 g / L of magnesium sulfate heptahydrate, 0.05 - 0.1 g / L of ferrous sulfate heptahydrate, and 0.01 - 0.03 g / L of zinc chloride (activating acyltransferase). The fermentation pH should be controlled to avoid being less than 5.5, otherwise it will inhibit the activity of glycolipid synthase. When the fermentation temperature is <28°C, the cell proliferation will be slow, and when it is >32°C, the proportion of sophorolipid lactonization will increase (the proportion of sophorolide should not be too high). After fermentation, add a small amount of food - grade polydimethylsiloxane (≤0.1 g / L) for defoaming to avoid affecting product separation.

[0035] Rhamnolipid can be prepared according to the following method:

[0036] (1) Mix glycerol with vegetable oil. The vegetable oil is sunflower oil and / or methyl palmitoleate, and the proportion of C16 - C18 in the vegetable oil is ≥80%. Add sterile water for formulation to make the initial concentration of glycerol 40 - 60 g / L and the initial concentration of vegetable oil 20 - 30 g / L. Then add 0.5 - 1.0 g / L of rhamnose, 2 - 3 g / L of ammonium nitrate, 1 - 2 g / L of urea, and 0 - 5 g / L of trace elements, and mix evenly to obtain the fermentation substrate.

[0037] (2) Transfer the fermentation substrate into a sterile fermenter and inoculate with OD according to 5 - 8% of the fermentation substrate volume 600Pseudomonas aeruginosa in the logarithmic mid-phase with a cell density of 8 - 10, initial fermentation pH of 7.0. During fermentation, the pH of the fermentation broth is maintained at 6.5 - 7.2 by adding NaOH solution, the temperature is maintained at 32 ± 0.5 °C, and the dissolved oxygen is controlled at 20% - 50% saturation. The dissolved oxygen is controlled at 50% within 0 - 12 h of fermentation to promote cell proliferation, and at 30 - 40% during 12 - 36 h of fermentation to induce the synthesis of metabolites; after 36 h of fermentation, the dissolved oxygen is controlled at 20% to promote the extension of hydrophobic chains; when the residual carbon content is less than 25 g / L, it is supplemented at a rate of 3 - 5 g / (L·h) until the total carbon content ≤ 180 g / L, and 10 - 15 g / L of sunflower oil is supplemented in two portions at 24 h and 48 h of fermentation; when the residual sugar drops to 25 g / L, glycerol is fed at a rate of 5 - 8 g / (L·h) until the total carbon content ≤ 180 g / L, and 10 - 15 g / L of vegetable oil is supplemented in two portions at 24 h and 48 h of fermentation; nitrogen supplementation is stopped after 36 h of fermentation.

[0038] (3) Stop fermentation after 96 h of fermentation or when the residual glycerol is detected to be < 2 g / L and the rhamnolipid concentration reaches 45 - 60 g / L.

[0039] (4) Introduce the fermentation broth into a foam column to enrich rhamnolipid. After adjusting the pH of the enriched product to 2.0 with dilute hydrochloric acid, let it stand at 4 °C for 20 - 30 h, and centrifuge to collect the precipitate; use a three-phase system composed of n - hexane - ethanol - water with a volume ratio of 3:5:2 for shaking extraction 2 - 4 times, combine the organic phases, remove the solvent by vacuum distillation, and ultrafiltrate and concentrate through a 100 kDa ceramic membrane to remove macromolecular impurities. The concentrated solution is freeze - dried to obtain rhamnolipid.

[0040] The trace elements in step (1) include: 2.0 - 3.0 g / L of ammonium dihydrogen phosphate, 0.8 - 1.2 g / L of magnesium sulfate heptahydrate, 0.05 - 0.08 g / L of ferrous sulfate heptahydrate, 0.02 - 0.05 g / L of calcium chloride (enhancing cell membrane permeability). The fermentation pH should be controlled to avoid being less than 6.3, otherwise it will inhibit the activity of rhamnosyltransferase; when the fermentation temperature is < 30 °C, the yield will decrease significantly, and when it is > 34 °C, the proportion of mono - rhamnolipid will increase by about 20%.

[0041] The extract of Astragalus membranaceus is the water extract of Astragalus membranaceus and can be extracted according to the following method: Select the main root of Astragalus membranaceus with a diameter ≥ 0.8 cm and the rhizome intact. After removing the mildewed, insect-eaten parts and impurities, air-dry it to a moisture content of 8 - 12%, cut it into thin slices of 3 - 5 mm, and under nitrogen protection, carry out air-flow pulverization at a temperature until the D90 particle size ≤ 180 μm. Add water at a ratio of 1:10 → 1:8 → 1:6 for three times of extraction. Between each extraction, use citric acid to adjust the pH to a weakly acidic environment of 6.5 - 7.0 to inhibit the dissolution of pectin, and the time interval between two extractions is less than 15 min to avoid serious oxidation of Astragalus membranaceus in the air; keep the extraction temperature at a slightly boiling state of 90 - 95 °C, extract for 60 - 90 min each time, and conduct on-line conductivity detection during the extraction process. When the conductivity change rate < 5 μS / cm·min, it is determined as the extraction end point; after each extraction, successively carry out treatments such as filtration through a 200-mesh sieve to remove residues, precision filtration, and adsorption of impurities by XAD-16 resin. Then concentrate the filtrate under reduced pressure at 60 - 65 °C so that the relative density of the concentrated solution at 60 °C is 1.15 - 1.20 (corresponding to the refractive index at 20 °C of 1.380 - 1.395).

[0042] In the water extract of Astragalus membranaceus with a relative density of 1.15 - 1.20 at 60 °C, the content of astragaloside IV ≥ 0.45 mg / g, and the polysaccharide (dextran equivalent) ≥ 18.5%. Before the first water extraction, first use water to soak Astragalus membranaceus slices. After the medicinal materials are put into the extraction tank, first add 5 times the amount of water, and adopt the hot and cold alternating soaking method (stepwise heating from 40 °C → 60 °C → 80 °C) to increase the micropore opening degree of the cell wall by 70%. During the extraction process, start stirring (20 - 30 rpm) to avoid the temperature aggregation and charring of some medicinal materials.

[0043] The yeast / zinc ferment can be prepared according to the following method:

[0044] Step 1: Select Saccharomycopsis fibuligera as the fermentation bacterium (its zinc tolerance is as high as 15 mM, the synthesis ability of intracellular metallothionein (MT) is 3 times that of Saccharomyces cerevisiae, and its cell wall is thinner, which is used to release the subsequent contents), and prepare a fermentation medium containing 75 - 85 g / L glucose and 12 - 20 g / L corn steep liquor. The C / N ratio in the medium is 22 - 28:1;

[0045] Step 2: Transfer the medium into a sterile fermentation tank, inoculate Saccharomycopsis fibuligera, the initial pH of fermentation is 5.5 - 5.8, the fermentation temperature is 30 °C ± 0.5, and the dissolved oxygen is 28 - 32% saturation; during the fermentation process, add zinc sulfate in segments. The initial concentration of zinc sulfate is 6 mM, and add zinc sulfate at 24 h of fermentation to make the total concentration of zinc sulfate reach 12 mM; during the fermentation process, when the yeast reaches the mid-logarithmic growth phase, add zinc sulfate (at this time, the zinc transmembrane efficiency is the highest), and the concentration of zinc sulfate in the fermentation broth does not exceed 12 mM;

[0046] Step 3: Fermentation ends when it reaches 72 h or when the zinc accumulation in yeast cells no longer increases.

[0047] Step 4: Adjust the cell suspension to pH 7.0, keep it at 40 - 45 °C for 2 - 3 h to activate the endogenous protease to cause partial autolysis of yeast, then add 1800 - 2200 U / L of lywallzyme, 400 - 500 U / L of neutral protease, and 50 - 80 U of chitinase, and then carry out enzymatic hydrolysis at 37 °C ± 1, pH 6.5 - 6.8 for 3 - 6 h. Ultrasonic-assisted enzymatic hydrolysis is used during the enzymatic hydrolysis process; when the flow cytometer detection shows that the cell wall breaking rate ≥ 95%, stop the enzymatic hydrolysis.

[0048] Step 5: Use a centrifuge to centrifuge at 10000 - 12000 rpm and 15 °C to remove cell debris, collect the supernatant, further remove proteins with an ultrafiltration membrane with a molecular weight cut-off of 100 kDa, and then carry out nanofiltration concentration to obtain a concentrated solution with a relative density of 1.18 - 1.22 at 20 °C (detected by the refractive index method at 20 °C, and at this time the dry matter content in the fermentation extract is 25 - 30%). Finally, add 0.1% trehalose + 0.05% ascorbyl palmitate (antioxidant) to the concentrated solution, carry out pasteurization at 72 °C for 15 s, and fill it aseptically for standby.

[0049] The three raw materials used in the present invention have the following characteristics respectively:

[0050] 1. Glycolipid biosurfactant:

[0051] It has an amphiphilic molecular structure, with a glycosyl hydrophilic head + a fatty acid hydrophobic tail, and the critical micelle concentration (CMC) is as low as 20 - 50 mg / L. It can be 100% biodegradable, and the natural degradation rate within 28 days > 98% (OECD 301D standard). Among them, the acid-type glycolipid destroys the bacterial membrane potential through electrostatic adsorption (MIC is as low as 0.1 - 1.0 μg / mL), showing antibacterial properties.

[0052] 2. Extract of Astragalus membranaceus var. mongholicus

[0053] Its core components are astragaloside IV, astragalus polysaccharide (APS), flavonoids, etc. It has immune regulation, promotes the expression of tight junction protein (Claudin-1) in keratinocytes, reduces the transepidermal water loss rate, has anti-allergic and soothing effects (inhibits histamine release), antioxidant, free radical scavenging, thick and thin circulation and repair effects, and accelerates wound healing. Its flavonoids and saponins have certain antibacterial and anti-inflammatory effects, enhance the cell membrane permeability, and assist in antibacterial efficacy.

[0054] 3. Yeast / zinc ferment

[0055] Zinc binds to metallothionein (MT), and its bioavailability is increased by more than 3 times compared to inorganic zinc. The zinc in yeast / zinc fermentate forms nano-micelles with a particle size of 50 - 80 nm, which can continuously release zinc, have antioxidant effects, inhibit inflammation, inhibit Malassezia, and promote wound repair. Zinc is an essential trace element for the human body and is widely distributed in various tissues of living organisms. More than 70 zinc-containing enzymes and non-enzymatic proteins in the human body rely on zinc atoms to exert their active functions. The zinc element content in the serum of skin disease patients is significantly lower than that of the normal population. Supplementing zinc can effectively prevent and treat skin diseases such as psoriasis, seborrheic dermatitis, and acne.

[0056] Mix the above three components to achieve "interface regulation and cleaning, immune repair and conditioning, yeast zinc targeted delivery, and flora regulation", and construct a full-link scalp improvement and a synergistic system of "cleaning - protection - regeneration".

[0057] The following combines the examples of the present invention to illustrate the solution. The sophorolipid and rhamnolipid used in the examples are from four types of glycolipids produced by Shanghai Bokoo Biotechnology Co., Ltd., namely Ecolife SL-02, Ecolife SL-03, Ecolife RP-01, and Ecolife RS-20; Ecolife SL-02 is mainly lactone-type sophorolipid, Ecolife SL-03 is mainly acid-type sophorolipid, Ecolife RP-01 is rhamnolipid, and Ecolife RS-20 is a mixture of sophorolipid and rhamnolipid.

[0058] Example 1

[0059] This example provides an antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition, which is obtained by combining sophorolipid, Astragalus membranaceus extract, and yeast / zinc fermentate. The relative density of the Astragalus membranaceus extract at 60 °C is 1.15, and the relative density of the yeast / zinc fermentate at 20 °C is 1.18. The sophorolipid is a combination of Ecolife SL-03 type sophorolipid and Ecolife SL-02 type sophorolipid produced by Shanghai Bokoo Biotechnology Co., Ltd., where the proportion of Ecolife SL-03 type sophorolipid is 15 wt%, and the proportion of Ecolife SL-02 type sophorolipid is 85%. The following experiments verify the antibacterial effects of compositions with different combination ratios and single components on Propionibacterium acnes (ATCC 6919). According to different combination ratios, the following three composition samples are obtained:

[0060] Sample 1#: Sophorolipid and Astragalus membranaceus extract are combined at a mass ratio of 1:1.

[0061] Sample 2#: Sophorolipid, Astragalus membranaceus extract, and yeast / zinc fermentate are combined at a mass ratio of 1:1:0.10.

[0062] Sample 3#: Sophorolipid and Astragalus membranaceus extract are combined at a mass ratio of 2:1.

[0063] Sample 4#: Sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment are combined at a mass ratio of 2:1:0.05.

[0064] Sample 5#: Sophorolipid and Astragalus membranaceus extract are combined at a mass ratio of 3:1.

[0065] Sample 6#: Sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment are combined at a mass ratio of 3:1:0.10.

[0066] Antibacterial test against Propionibacterium acnes

[0067] Propionibacterium acnes was inoculated into Brain Heart Infusion (BHI) medium and anaerobically cultured at 37 °C in a carbon dioxide incubator for 2 - 3 days. The bacterial solution was diluted 10 -5～-6 times with 0.9% sterile normal saline and used as the indicator bacteria for the antibacterial test. Different composition samples and single components were respectively prepared into stock solutions. Different amounts of the stock solutions were taken and added to Brain Heart Infusion (BHI) agar medium to make BHI agar medium plates containing different samples and gradient concentrations. After solidification, 100 μL of the Propionibacterium acnes bacterial suspension was pipetted onto the plates. After spreading evenly, the culture dishes were inverted and placed in a 37 °C carbon dioxide incubator for anaerobic culture for 12 h. The number and size of colonies on the plates were observed. Multiple replicates were set for each test sample, and the lowest exposure concentration at which the antibacterial rate reached 90% within 12 h was recorded for each group. The following formula was used to calculate the antibacterial combination index of each component in different samples to evaluate whether there was a synergistic antibacterial effect among the components in the samples for the antibacterial experiment. The detection and calculation results are shown in Table 1.

[0068] Calculation method for the combined index (CI) of two components:

[0069]

[0070] Among them, (D)1 and (D)2 are the actual concentrations of the two components when the proliferation inhibition rate of the composition reaches X; (Dx)1 and (Dx)2 are the component concentrations when the two components are used alone and the proliferation inhibition rate reaches X.

[0071] Calculation method for the combined index (CI) of three components:

[0072]

[0073] (D)1, (D)2, and (D)3 are the actual concentrations of the three components when the composition reaches a proliferation inhibition rate of X; (Dx)1, (Dx)2, and (Dx)3 are the component concentrations of the three components when used alone to reach a proliferation inhibition rate of X.

[0074] The Calcμsyn 2.0 software was used to analyze the component synergy: CI < 1 indicates mutual synergy between the two drugs; CI = 1 indicates additive effects of the two drugs; CI > 1 indicates antagonistic effects of the two drugs; CI < 0.3 indicates strong synergy between the two components.

[0075] Table 1:

[0076]

[0077]

[0078] As can be seen from Table 1, when using a combination of sophorolipid, Astragalus membranaceus extract, and yeast / zinc ferment, it can inhibit Propionibacterium acnes more effectively than single components or combinations of sophorolipid and Astragalus membranaceus extract. Moreover, the smaller the combined inhibition index CI value, the stronger the synergistic antibacterial effect between the components.

[0079] In order to further shorten the antibacterial time against Propionibacterium acnes and accelerate the onset speed, in the experiment, the concentrations of the above-mentioned Sample 2#, Sample 4#, and Sample 6# were increased to 1% respectively, and the shortest time required for their bacteriostatic rate against Propionibacterium acnes to reach ≥ 90% was tested. The test results are shown in Table 2.

[0080] Table 2:

[0081] Sample Exposure concentration (%) with 90% antibacterial rate Shortest time consumption Sophorolipid 1.0 45 min Yeast / Zinc ferment 1.0 24 min Sample 2# 1.0 3 min Sample 4# 1.0 6 min Sample 6# 1.0 5 min

[0082] As can be seen from Table 2, the mixture composed of sophorolipid, Astragalus membranaceus extract, and yeast / zinc ferment can quickly inhibit Propionibacterium acnes, with a short onset time and significant bacteriostatic effect. It can take effect faster without the need for long-term contact of the composition with the scalp. As a shampoo product, it has irreplaceable advantages.

[0083] Example 2

[0084] This embodiment provides an antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition, which is obtained by combining sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment. The relative density of the Astragalus membranaceus extract at 60 °C is 1.2, and the relative density of the yeast / zinc ferment at 20 °C is 1.2. The sophorolipid is a combination of Ecolife SL-03 sophorolipid and Ecolife SL-02 sophorolipid produced by Shanghai Bokoo Biotechnology Co., Ltd., where the proportion of Ecolife SL-03 sophorolipid is 25 wt%, and the proportion of Ecolife SL-02 sophorolipid is 75%. The following experiments verify the antibacterial effects of compositions with different combination ratios and single components on Malassezia furfur (ATCC 14251). According to different combination ratios, the following three composition samples are obtained respectively:

[0085] Sample 7#: Sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment are combined at a mass ratio of 1:1:0.10.

[0086] Sample 8#: Sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment are combined at a mass ratio of 2:1:0.05.

[0087] Sample 9#: Sophorolipid, Astragalus membranaceus extract and yeast / zinc ferment are combined at a mass ratio of 3:1:0.1.

[0088] Antibacterial test of Malassezia furfur

[0089] Dissolve the commercially available YPD agar and liquid medium in water as required and sterilize at 121 °C for 15 minutes. After the YPD agar medium cools down and is poured into plates, invert it after solidification.

[0090] Inoculate the glycerol tube strain of Malassezia furfur stored at -80 °C on the YPD agar plate and culture it at 29 °C for 2 - 5 days until single colonies grow, then set aside; pick the cultured single colonies and inoculate them into the YPD liquid medium, shake culture at 220 rpm and 29 °C for 2 - 5 days, and then dilute serially to 10 -5 ~10 -6 of the Malassezia furfur bacterial suspension as the antibacterial test indicator bacteria. Prepare the mother liquors of different composition samples and single components respectively, take different amounts of the mother liquors in yeast extract peptone dextrose (YPD) agar medium to make YPD medium plates containing different samples and gradient concentrations. After solidification, pipette 100 μL of the Malassezia furfur bacterial suspension onto the plate, spread it evenly and culture it in an incubator at 29 °C for 12 h. Observe the number and size of the colonies on the plate. Set multiple replicates for each test sample, record the lowest exposure concentration at which the antibacterial rate reaches 90% within 12 h for each group, and calculate the antibacterial combination index of each component of different samples using the formula of Equation (II) to evaluate whether there is a synergistic antibacterial effect among the components in the samples of the antibacterial experiment. The detection and calculation results are shown in Table 3.

[0091] Table 3:

[0092]

[0093] As can be seen from Table 3, when sophorolipid, Astragalus membranaceus extract, and yeast / zinc ferment are used in combination, they can inhibit Malassezia furfur more effectively than single components or the combination of sophorolipid and Astragalus membranaceus extract. Moreover, the smaller the combined inhibition index CI value, the stronger the synergistic antibacterial effect between the components.

[0094] To further shorten the antibacterial time against Malassezia furfur and accelerate the onset of action, in the experiment, the concentrations of Sample 6#, Sample 7#, and Sample 8# were increased to 1.0% respectively, and the shortest time required for the antibacterial rate against Malassezia furfur to reach ≥90% under the test concentration was measured. The test results are shown in Table 4.

[0095]

[0096]

[0097] As can be seen from Table 4, the mixture composed of sophorolipid, Astragalus membranaceus extract, and yeast / zinc ferment can rapidly inhibit Malassezia furfur, with a short onset time and significant antibacterial effect. It can take effect faster without the need for long-term contact of the composition with the scalp. As a shampoo product, it has irreplaceable advantages.

[0098] Example 3

[0099] This example provides an antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition, which is obtained by combining sophorolipid, Astragalus membranaceus extract, and yeast / zinc ferment. The relative density of the Astragalus membranaceus extract at 60°C is 1.2, and the relative density of the yeast / zinc ferment at 20°C is 1.2. The following experiments verify the removal effects of compositions with different combination ratios and single components on the biofilm of Malassezia furfur (ATCC 14251). According to the different types of sophorolipids used, the following two composition samples are obtained:

[0100] Sample 10#: Ecolife SL-02 type sophorolipid (lactone type), Astragalus membranaceus extract, and yeast / zinc ferment are combined in a mass ratio of 1:1:0.10.

[0101] Sample 11#: Ecolife SL-03 type sophorolipid (acid type), Astragalus membranaceus extract, and yeast / zinc ferment are combined in a mass ratio of 1:1:0.10.

[0102] Biofilm, also known as biological membrane, refers to an organized group of bacteria or fungi attached to the surface of inanimate or animate objects and wrapped by extracellular macromolecules. They secrete extracellular polymeric matrix and have different tolerances to antibacterial compounds compared with the same kind of planktonic microorganisms. The main component of biofilm, polysaccharide-protein complex, can wrap the bacterial cells, and the cells adhere to each other to form a complex. It has a specific, irreversible structure and adheres to the surface of the catheter or lesion like a membrane. Biofilm can form on various surfaces such as living tissues and medical devices. Once formed, it is very stubborn and extremely difficult to remove. Biofilm can significantly improve the resistance of microorganisms to harsh environments and disinfectants and continuously accept planktonic microorganisms. In this example, the removal effects of different compositions on the biofilm of Malassezia furfur (ATCC 14251) were tested.

[0103] Experimental method: After Malassezia furfur was cultured on a YPD agar plate at 29 °C for 24 h, a single colony was picked and transferred to a YPD liquid medium. After shaking culture at 29 °C and 200 rpm for 24 h, it was diluted to 1% with sterile normal saline. 1 mL of the diluted bacterial suspension was pipetted into a 6-well culture plate, and a sterilized cover glass was placed at the same time. After static culture at 29 °C for 48 h, Malassezia furfur formed a biofilm on the cover glass. The culture medium was carefully aspirated, and the cover glass was washed three times with sterile PBS buffer. Then, aqueous solutions of three substances, Ecolife SL-02 type sophorolipid with concentrations of 0.325 g / L, 0.65 g / L, 1.3 g / L, 2.6 g / L, 5.2 g / L (unit: g / L), sample 10#, and sample 11#, were respectively injected. After static treatment in an incubator for 90 min, the cover glass was taken out, washed three times with sterile water, stained with 0.01% crystal violet, and observed under a 100-fold microscope for the removal effect of sophorolipid on the biofilm on the cover glass.

[0104] See Figure 1-2As shown, they are SEM images of removing Malassezia furfur biofilm for Sample 10# and Sample 11# respectively. The larger the blue area, the more intact the biofilm. Sample 10# has a stronger ability to remove the biofilm. It can significantly remove the Malassezia furfur biofilm at a concentration of 0.65 g / L, and can basically remove all the Malassezia furfur biofilm at a concentration of 2.6 g / L. The ability of Sample 11# to remove the Malassezia furfur biofilm is slightly worse. The biofilm area starts to decrease significantly only when the concentration is greater than 0.65 g / L and close to 1.3 g / L. When using Ecolife SL-02 type sophorolipid alone to remove the Malassezia furfur biofilm, the exposure concentration of Ecolife SL-02 type sophorolipid must reach at least 0.78 g / L or more to show the effect, and to achieve complete removal, the exposure concentration of sophorolipid must be ≥ 3.125 g / L. Thus, it can be seen that after the lactone-type sophorolipid or acid-type sophorolipid is compounded with Astragalus membranaceus extract and yeast / zinc ferment, the Malassezia furfur biofilm can be quickly removed at a lower concentration. While the lactone-type sophorolipid alone has a poor effect on removing the Malassezia furfur biofilm and requires a higher concentration. Among them, the composition compounded by "lactone-type sophorolipid, Astragalus membranaceus extract, yeast / zinc ferment" has the best effect in removing the Malassezia furfur biofilm.

[0105] Example 4

[0106] In this example, the combined Sample 10# prepared in Example 3 is continued to be used to test its anti-allergic and skin-soothing functions.

[0107] Hyaluronic acid (HA) is a major component of the extracellular matrix and interstitial fluid, and plays an important role in processes such as skin elasticity, cell adhesion, wound healing, and angiogenesis. Research shows that HA is also closely related to allergic reactions and has a great relationship with skin sensitivity. Hyaluronidase (HAase), as a proteolytic enzyme that specifically decomposes HA, is commonly used in allergy-related research. The common allergic symptoms of redness, swelling, itching, and pain are mainly caused by histamine, and the common clinical medications are mainly antihistamines. Research shows that there is a good correlation between the inhibition of hyaluronidase activity and the inhibition of histamine release from mast cells. The active ingredients that inhibit histamine often also show relatively obvious hyaluronidase inhibition activity. Therefore, as a participant in type I allergic reactions, hyaluronidase is often used to explore anti-allergic active ingredients in vitro.

[0108] Test method for the soothing and anti-allergic effect of the composition:

[0109] Add 0.1 mL of 2.5 mmol / L CaCl2 to 0.5 mL of hyaluronidase (500 U / mL), and incubate at 37 °C for 20 min; add 0.5 mL of sample 10#, and incubate at 37 °C for 20 min; add 2 mL of sodium hyaluronate (0.4 mg / mL), and incubate at 37 °C for 40 min. Then add 1 mL of acetylacetone solution (1.5 mL of acetylacetone dissolved in 50 mL of 1.25 mol / L sodium carbonate solution, freshly prepared), incubate at 90 °C for 1 h, cool with water, slowly add 10 mL of ethanol with a concentration of 96%, and then add 1.0 mL of Ehrlich reagent (1.6 g of DMAB dissolved in 30 mL of concentrated hydrochloric acid and 30 mL of ethanol with a mass fraction of 96%). Mix well, let stand at room temperature for 1 h, and then measure the absorbance at 530 nm.

[0110] Hyaluronidase inhibition rate (%) = [(C - D) - (A - B)] / (C - D) × 100%

[0111] In the formula:

[0112] A: OD value of the sample solution in the sample group (hyaluronidase + sample + sodium hyaluronate);

[0113] B: OD value of the sample solution in the sample control group (acetic acid buffer + sample + acetic acid buffer);

[0114] C: OD value of the control solution (hyaluronidase + deionized water + sodium hyaluronate);

[0115] D: OD value of the control blank (acetic acid buffer + deionized water + acetic acid buffer).

[0116] The test results are as Figure 3 shown. Among them, sample 10# has an inhibitory effect on the activity of hyaluronidase, and when the sample concentration is about 2%, the inhibition rate reaches 75% (see Figure 3 ), indicating that the composition has good soothing and anti-allergic effects on the skin.

[0117] Example 5

[0118] Use sample 2# prepared in Example 1 to prepare shampoo, and the shampoo ingredients are shown in Table 5.

[0119] Table 5

[0120]

[0121]

[0122] The preparation method of the above shampoo is as follows: Mix coconut fatty acid monoethanolamide (CMEA), sodium lauryl ether sulfate (AES), chelating agent, and deionized water, heat to 85°C and stir to dissolve, then add pearlescent agent and stir to dissolve evenly. Cool down to 60°C, and successively add 1,2 - hexanediol and cocamidopropyl betaine (CAB - 35), and mix and stir evenly. Continue to cool down to 40°C, add the composition of Sample 2# (the mass ratio of sophorolipid: Astragalus membranaceus extract: yeast / zinc ferment is 1:1:0.1), add an appropriate amount of sodium chloride, and then add citric acid to adjust the pH to 5.5 - 6.5, stir evenly, sample and discharge to obtain the finished product.

[0123] Example 6

[0124] In this example, the shampoo was prepared using Sample 4# prepared in Example 1. The ingredients of the shampoo are shown in Table 5, except that the "main active ingredient" was replaced with Sample 4#, and the mass ratio of sophorolipid: Astragalus membranaceus extract: yeast / zinc ferment in Sample 4# was 2:1:0.05. The preparation method refers to Example 5.

[0125] Example 7

[0126] In this example, the shampoo was prepared using Sample 6# prepared in Example 1. The ingredients of the shampoo are shown in Table 5, except that the "main active ingredient" was replaced with Sample 6#, and the mass ratio of sophorolipid: Astragalus membranaceus extract: yeast / zinc ferment in Sample 6# was 3:1.0:0.1. The preparation method refers to Example 5.

[0127] Comparative Example 1

[0128] In this comparative example, based on Example 5, the yeast / zinc ferment was replaced with zinc salt ZPT, and the content of ZPT in the shampoo was 0.1 wt%. The other components and their contents remained unchanged.

[0129] Comparative Example 2

[0130] In this comparative example, based on Example 5, the Astragalus membranaceus extract was removed (i.e., not added), and the other components and their contents remained unchanged.

[0131] Comparative Example 3

[0132] In this comparative example, based on Example 5, the sophorolipid was replaced with an equal amount of sulfate SLES, and the content of sulfate in the shampoo was 1.0%. The other components and their contents remained unchanged.

[0133] Microbial Flora Regulation Test

[0134] Recruit volunteers to use the shampoo products of Examples 5 - 7 and Comparative Examples 1 - 3. The inclusion criteria are: obvious visible dandruff and excessive scalp sebum secretion. Select 30 people with obvious visible dandruff and excessive scalp sebum secretion, and randomly divide them into 6 groups, with 5 people in each group. Wash hair every day, with a dosage of 15 - 20 mL each time. After wetting the scalp with warm water each time, apply the shampoo and gently massage for 5 minutes. After washing the hair, dry it. Conduct a continuous 7 - day washing test, and during this period, do not use other hair care products and do not perform any hair care or beauty treatments. After 7 days of use, then do not wash hair for the next 3 days, and then feedback their feelings. Subjectively score according to whether there is a sense of tightness, whether there is obvious dandruff, whether there are follicular acne, and whether there is no scalp itching. The scoring criteria are as follows:

[0135] No scalp tightness, no obvious dandruff, no follicular acne, no scalp itching, score 5 points;

[0136] If there is one of scalp tightness, obvious dandruff, follicular acne, and scalp itching, score 2 points;

[0137] If there are more than 2 of scalp tightness, obvious dandruff, follicular acne, and scalp itching, score 0 points.

[0138] Positive feedback degree = actual score / theoretical total score × 100%

[0139] The scoring results are shown in Table 6.

[0140] Table 6

[0141]

[0142]

[0143] As can be seen from Table 6, the positive feedback of the shampoo of Examples 5 - 7 prepared with the composition of the present invention reaches 100%, and has received unanimous praise. After the shampoo of Comparative Example 3 uses the sulfate SLES surfactant, the volunteers even gave a score of zero, indicating that their hair problems have not been significantly and substantially improved. After replacing the yeast / zinc with the ordinary zinc salt ZPT in Comparative Example 1, some volunteers gave high scores but some still felt that the effect was not obvious, so they gave lower scores.

[0144] Recruit 12 more volunteers with strong scalp sebum secretion and severe scalp acne (folliculitis) for long-term testing. There are clearly visible red and swollen bumps (1-5 mm in diameter) on the scalps of these volunteers, with yellowish-white pustules in the center. The testing method is as follows: Randomly divide the volunteers into 4 groups, with 3 in each group. Before the test, conduct scalp health examinations on these volunteers, including initial examinations and records of the ratio of Propionibacterium / acne Staphylococcus (by 16S rRNA sequencing), transepidermal water loss rate, red and swollen bumps, etc. Then each group uses the shampoos of Example 5 and Comparative Examples 1-3 of the present invention to wash their hair continuously for 1 month, washing every other day, with a dosage of 15-20 mL / time. After wetting the scalp with warm water each time, apply the shampoo and gently massage for 5 minutes. After washing, dry the hair. After 1 month, check the scalp health status again according to the aforementioned method and record the average value of each group. Record the scalp health conditions before and after the test as follows:

[0145] Table 7:

[0146]

[0147]

[0148] Studies have shown that the ratio of Propionibacterium to Staphylococcus in healthy skin is approximately 70-80% and 5-10%. When the ratio of Staphylococcus > 15%, or Propionibacterium < 50%, it is considered imbalanced. The symbiotic Staphylococcus epidermidis is the main type (pathogenic Staphylococcus aureus < 1%). The proportion of Staphylococcus aureus > 5%: significantly increases the risk of infection (such as folliculitis, atopic dermatitis). The TEWL (transepidermal water loss rate) of a healthy scalp is 8-12 g / m 2 / h, while the TEWL of a folliculitis or acne scalp is between 15-25.

[0149] In summary, the shampoo containing the antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition of the present invention can achieve dual regulation of the scalp microecology-barrier, help regulate the ratio of Propionibacterium / acne Staphylococcus in a healthy and balanced direction, and reduce the transepidermal water loss rate of the scalp. This shows that the shampoo containing the antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition of the present invention can effectively repair damaged scalps, restore the natural barrier of the scalp, and improve the integrity of the scalp cutin layer.

[0150] It should be noted that although the composition of the present invention has been proven to have a good inhibitory effect on Propionibacterium acnes strains (such as ATCC 6919) in the previous embodiments, it is not sensitive to Propionibacterium acnes subtypes of skin symbiotic type (such as HL043, phylotype IB), etc. These subtypes of Propionibacterium acnes are sometimes beneficial bacteria that can synthesize antibacterial peptides and secrete antioxidant substances to inhibit the reproduction of pathogenic Staphylococcus. Through the active ingredients in the shampoo, the pathogenic Staphylococcus (such as Staphylococcus aureus) can be reduced, the ecological niche can be released, the symbiotic Propionibacterium acnes can be promoted to proliferate, the dominant pathogenic bacteria (Staphylococcus) can be reduced, the overall microbial flora uniformity can be improved, the scalp microbial environment can be restored to balance, and the scalp health can be restored. Therefore, the care of the shampoo for the scalp is to change the scalp from "microbial flora imbalance" to "functional balance", focusing on the protection and balance of functional microbial flora rather than simply antibacterial.

[0151] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions recorded in the foregoing embodiments, or perform equivalent replacements on some or all of the technical features; and these modifications or replacements, or when the technical features in the above embodiments do not conflict with each other, can be combined in the manner recorded in the embodiments, and these modifications, replacements or combinations do not make the essence of the corresponding technical solutions deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition, characterized in that, It contains a biosurfactant, an extract of Astragalus membranaceus, and a yeast / zinc ferment; they are combined in a mass ratio of 1-3: 0.5-1.5: 0.05-0.10; wherein, the extract of Astragalus membranaceus is an aqueous extract of Astragalus membranaceus, and its relative density at 60 °C is 1.15-1.20; the relative density of the yeast / zinc ferment at 20 °C is 1.18-1.

22.

2. The antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition according to claim 1, characterized in that, The biosurfactant, the extract of Astragalus membranaceus, and the yeast / zinc ferment are combined in a mass ratio of 1:1:0.10; or in a mass ratio of 2:1:0.05; or in a mass ratio of 3:1.5:0.

1.

3. The antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition according to claim 1 or 2, characterized in that The biosurfactant is sophorolipid and / or rhamnolipid.

4. The antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition according to claim 1, wherein The yeast / zinc ferment is obtained by adding inorganic zinc to the culture medium during the growth of yeast, utilizing the absorption and transformation of zinc by yeast to obtain zinc-rich yeast, and then releasing the intracellular substances of yeast through a biological enzymolysis technique, and collecting to obtain the yeast / zinc ferment.

5. The antibacterial, anti-dandruff, oil-control, soothing and anti-allergic composition according to claim 1, wherein The sophorolipid is obtained by inoculating Candida bombicola on glucose and vegetable oil as substrates and fermenting and separating and purifying under sterile conditions; the mass percentage of acid-type sophorolipid in the sophorolipid is 0-30%, and the mass percentage of lactone-type sophorolipid is 100-70%.

6. The bacteriostatic, anti-dandruff, oil-control, soothing and anti-allergic composition according to claim 1, wherein The preparation method of the rhamnolipid is: inoculating Pseudomonas microorganisms in a carbon source and vegetable oil, and fermenting and separating and purifying under sterile conditions; the carbon source is at least one of glucose, glycerol, and citric acid.

7. A hair washing and care product, characterized in that, It contains the antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition according to any one of claims 1-6.

8. The hair washing and care product according to claim 7, wherein The hair washing and care product uses the antibacterial, anti-dandruff, oil-control, soothing, and anti-allergic composition according to any one of claims 1-6 as the only active ingredient for anti-dandruff, antibacterial, and oil-control.

9. The hair washing and care product according to claim 7, wherein The hair washing and care product further includes one or more of a main surfactant, an auxiliary surfactant, a thickener, a co-solvent, a chelating agent, a preservative, a pearlescent agent, a conditioner, a buffer, a fragrance, and a pigment.

10. The hair washing and care product according to claim 7, characterized in that, The hair washing and care product, by mass percentage, has the following composition: 2.0-3% of CMEA, 15.0-18% of AES, 0.01-0.03% of EDTA-2Na, 2-3% of the composition, 0.4-0.6% of ethylene glycol distearate, 4-6% of cocamidopropyl betaine, 0.4-0.6% of 1,2-hexanediol, 0.1-3% of citric acid, 0.8-1.2% of sodium chloride; the balance is water.

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