Self-adaptive fixed bone marrow assembly type separation device

Abstract

The invention discloses a self-adaptive fixing bone marrow assembly type separating device which comprises a collecting pipe, a separating pipe and a core which are assembled and installed, a fixing hole channel in the core is used for fixing bones, and incomplete assembly is formed between the separating pipe and the core through a wedge-shaped key block and a wedge-shaped key groove. After interaction force is generated between the wedge-shaped key block and the wedge-shaped key groove, the core and the fixing hole channel of the core are extruded and deformed to tightly extrude the bone to be fixed, and a liquid carrying interval is formed. By adopting an assembly type centrifugal bone marrow separation mode, after the core and the separation pipe are assembled, interaction force which is positively correlated with the centrifugal rotating speed is generated between the wedge-shaped key block and the wedge-shaped key groove, so that the fixed hole channel of the core generates deformation quantity which is positively correlated with the centrifugal rotating speed, and the bone fixed in the core is tightly extruded; and a liquid carrying interval capable of being pre-filled with flushing liquid is formed, so that centrifugal self-adaptive fixation of bones and integration of flushing and centrifugal separation are achieved, the collection rate of bone marrow is effectively increased, and the inactivation rate of bone marrow cells is effectively reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of biological experimental equipment, and particularly relates to an adaptive fixed bone marrow assembled separation device. Background Art

[0002] Biological experimental bone marrow is mainly separated from the bone marrow cavity of the long bones, the cancellous spaces of flat bones and irregular bones of animals. The existing bone marrow separation schemes mainly use the centrifugation method. When separating bone marrow by the centrifugation method, usually multiple cut bones are placed in a small-sized inner centrifuge tube, and then the inner centrifuge tube is placed in a large-sized outer centrifuge tube to form a nested structure. A hole is drilled at the bottom of the inner centrifuge tube, and then the inner and outer centrifuge tubes are capped and placed obliquely in the centrifuge for centrifugation. During centrifugation, the bone marrow in the bones is separated from the inner wall of the bone marrow cavity under the centrifugal force and flows out from the lower end of the bones. The bone marrow flowing out of the bones is thrown away from the bones under the centrifugal force and falls onto the inner wall of the inner centrifuge tube, and gathers towards the bottom of the inner centrifuge tube under the centrifugal force to pass through the hole and enter the bottom of the outer centrifuge tube for collection.

[0003] Although this method of nested centrifugation of double centrifuge tubes for separating bone marrow can reduce the contact area between the bone marrow liquid and the vessels and improve the collection rate, during the implementation process, since multiple cut bones are directly placed in a small-sized centrifuge tube, it is easy for the multiple bones to move and squeeze each other and break under the centrifugal force, resulting in part of the bone marrow remaining in the bone fragments gap, or the hole being blocked by the bone fragments, making it difficult to collect part of the bone marrow by centrifugation. In addition, the liquid content in the bone marrow is usually small, and part of the viscous bone marrow adheres firmly to the inner wall of the marrow cavity and is difficult to be completely separated by centrifugation, resulting in part of the bone marrow remaining in the bone marrow cavity, thus affecting the collection rate of the bone marrow. And the adherent bone marrow is easy to inactivate due to less liquid content. Therefore, after centrifugation, usually a pressurizing device (such as a syringe, a pipette gun, etc.) is used in time to perfuse a liquid medium (such as a culture medium, PBS, etc.) into the bone marrow cavity to infiltrate and wash and separate the remaining bone marrow in the cavity, so as to further improve the collection rate of the bone marrow and maintain the activity of bone marrow cells. However, since most of the bone marrow in the marrow cavity has been separated by centrifugation, the liquid resistance in the marrow cavity decreases, so a large amount of liquid medium is required for flushing to separate the adherent bone marrow.

[0004] Therefore, for the existing device of nested centrifugation of double centrifuge tubes for separating bone marrow, because the inner centrifuge tube is difficult to independently fix multiple bones of different specifications, it is easy for the multiple bones to squeeze and break each other under the centrifugal force, affecting the collection rate, and because it is difficult to directly centrifuge and separate the adherent bone marrow and timely flushing and separation are required, the consumption of the liquid medium is large, and the operation difficulty is increased. Summary of the Invention

[0005] The object of the present invention is to provide an adaptively fixed bone marrow assembly separation device to solve the technical problems in the prior art that it is difficult to achieve the flushing and infiltration of liquid media during centrifugation, resulting in insufficient bone marrow collection rate and reduced cell activity.

[0006] To solve the above technical problems, the present invention specifically provides the following technical solutions:

[0007] An adaptively fixed bone marrow assembly separation device includes a collection tube and a separation tube sleeved inside the collection tube. The bottom of the separation tube extends downward and contracts to form a diversion channel. A core made of elastic material is clamped at the contraction of the separation tube. The separation tube and the core are closed to form a liquid-carrying interval. Inside the core, there are multiple uniformly distributed fixing channels for fixing bones.

[0008] Among them, on the inner wall of the separation tube, there are circumferentially arranged wedge-shaped key blocks. The outer wall of the core is recessed inward to form wedge-shaped key grooves corresponding to the wedge-shaped key blocks to complete the assembly with the separation tube. The inclination angle of the wedge-shaped key grooves is smaller than that of the wedge-shaped key blocks, so that the core is not completely fitted into the separation tube in the natural state.

[0009] The liquid-carrying interval is used to fill the flushing liquid. During centrifugation with the cover, under the action of centrifugal force, the bone marrow in the bones fixed in the fixing channels is flushed and separated, and then enters the bottom of the collection tube through the diversion channel.

[0010] As a preferred solution of the present invention, the number of the fixing channels is the same as that of the wedge-shaped key grooves, and the fixing channels are located between adjacent wedge-shaped key grooves. During centrifugation, the wedge-shaped key grooves on the core are squeezed against the wedge-shaped key blocks on the separation tube under the action of centrifugal force, and the fixing channels are squeezed under the reaction force of the wedge-shaped key blocks. After deformation, the fixing channels are conical to strengthen the fixation of the bones.

[0011] As a preferred solution of the present invention, the wedge-shaped key groove has two inner triangular surfaces. One side of the two inner triangular surfaces close to the axis of the core is butted to form an inner groove line. The distance between the inner groove line and the axis of the core gradually decreases from top to bottom.

[0012] The wedge-shaped key block has two outer triangular surfaces. One side of the two outer triangular surfaces away from the axis of the separation tube is butted to form an outer edge line. The distance between the outer edge line and the axis of the separation tube gradually decreases from top to bottom.

[0013] Among them, the slope of the outer edge line is greater than that of the inner groove line.

[0014] As a preferred embodiment of the present invention, the distance between the two inner triangular faces gradually increases from top to bottom, the distance between the two outer triangular faces gradually increases from top to bottom, and the increment of the distance between the two inner triangular faces from top to bottom is less than the increment of the distance between the two outer triangular faces from top to bottom.

[0015] As a preferred embodiment of the present invention, as the centrifugal speed increases, the wedge key groove and the wedge key block are more strongly pressed under the action of centrifugal force, causing a greater deformation of the fixed channel to adaptively strengthen the fixation of the bone following the centrifugal speed.

[0016] As a preferred embodiment of the present invention, the inner wall of the fixed channel is also made of an elastic material, and the inner diameter of the fixed channel is greater than the maximum outer diameter of the bone.

[0017] As a preferred embodiment of the present invention, the bottom of the separation tube converges towards the center and protrudes downward to form a nipple, and the diversion channel runs through along the axis of the nipple.

[0018] As a preferred embodiment of the present invention, a limiting structure is provided at the opening of the separation tube, and the limiting structure maintains a fixed relative position with the collection tube under the action of centrifugal force.

[0019] As a preferred embodiment of the present invention, the limiting structure is specifically a T-shaped ring with an outwardly protruding outer edge that abuts against the mouth of the collection tube. The T-shaped ring is in close contact with the collection tube under the action of centrifugal force and restricts relative movement between the separation tube and the collection tube.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] The present invention adopts an assembled centrifugal separation of bone marrow. After assembling the core and the separation tube, a positive correlation between the interaction force between the wedge key block and the wedge key groove and the centrifugal speed is generated, causing a positive correlation between the deformation of the fixed channel of the core and the centrifugal speed, tightly pressing the bone fixed inside it, and forming a liquid-carrying interval capable of pre-filling the flushing liquid to achieve the integrated centrifugal adaptive fixation, flushing, and centrifugal separation of the bone. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings described below are only exemplary, and those of ordinary skill in the art can also obtain other implementation drawings based on the provided drawings without creative efforts.

[0023] Figure 1Structural schematic diagram of the self - adaptive fixed bone marrow assembly - type separation device provided by the embodiment of the present invention;

[0024] Figure 2 Structural schematic diagram of the separation tube and the core part of the self - adaptive fixed bone marrow assembly - type separation device provided by the embodiment of the present invention;

[0025] Figure 3 Structural schematic diagram of the wedge - shaped key block and the wedge - shaped key groove part of the self - adaptive fixed bone marrow assembly - type separation device provided by the embodiment of the present invention;

[0026] Figure 4 Schematic diagram of the core being deformed under pressure of the self - adaptive fixed bone marrow assembly - type separation device provided by the embodiment of the present invention.

[0027] The reference numerals in the figure are respectively represented as follows:

[0028] 1 - Collection tube; 2 - Separation tube; 3 - Core;

[0029] 21 - Flow - guiding channel; 22 - Wedge - shaped key block; 23 - Nipple; 31 - Fixing channel; 32 - Wedge - shaped key groove;

[0030] 201 - Liquid - carrying section; 221 - Outer triangular surface; 222 - Outer edge line; 321 - Inner triangular surface; 322 - Inner groove line. Detailed implementation manners

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] As shown in Figure 1 、 Figure 2 The present invention provides a self - adaptive fixed bone marrow assembly - type separation device, including a collection tube 1 and a separation tube 2 sleeved inside the collection tube 1. The bottom of the separation tube 2 extends downward and contracts to form a flow - guiding channel 21. At the contraction of the separation tube 2, a core 3 made of an elastic material is clamped. The separation tube 2 and the core 3 are closed to form a liquid - carrying section 201. Inside the core 3, there are a plurality of uniformly distributed fixing channels 31 for fixing bones.

[0033] Among them, on the inner wall of the separation tube 2, there are circumferentially arranged wedge - shaped key blocks 22. The outer wall of the core 3 is recessed inward to form wedge - shaped key grooves 32 corresponding to the wedge - shaped key blocks 22 to complete the assembly with the separation tube 2. The inclination angle of the wedge - shaped key groove 32 is smaller than the inclination angle of the wedge - shaped key block, so that the core 3 is not completely fitted into the separation tube 2 in the natural state (as shown inFigure 1 as shown

[0034] The liquid-carrying section 201 is used to fill the flushing liquid. When the cover is centrifuged, under the action of centrifugal force, the flushing liquid flushes and separates the bone marrow fixed in the fixed channel 31 of the bone, and then enters the bottom of the collection tube 1 through the diversion channel 21.

[0035] The self-adaptive fixed bone marrow assembly separation device of the present invention uses the nested assembly of the collection tube 1 and the separation tube 2 to provide a collection area, and uses the nested assembly of the core 3 and the separation tube 2 to provide an independent bone fixation area, that is, multiple bones are independently fixed through multiple fixed channels 31 on the core 3, and the core 3 and the separation tube 2 are incompletely fitted by using a wedge key groove 32 and a wedge key block 22. After the core 3 moves to the bottom of the separation tube 2 and is tightly assembled, the wedge key block 22 squeezes the wedge key groove 32, causing the core 3 to deform and transmit to the fixed channel 31, realizing the tight fixation of bones with different specifications.

[0036] At the same time, since the fixed channel 31 is in close contact with the bone fixed therein, a liquid-carrying section 201 is formed in the separation tube 2 above the core 3, that is, the flushing liquid can be pre-filled into the liquid-carrying section 201 before centrifugation. Thus, during capping centrifugation, the flushing liquid in the liquid-carrying section 201 flows along the medullary cavity under the action of centrifugation, realizing the infiltration and flushing of the bone marrow in the medullary cavity, that is, integrating the functions of centrifugal separation and flushing separation, thereby maintaining the activity of bone marrow cells and effectively improving the collection rate and reducing the consumption of flushing liquid.

[0037] During the centrifugation process, as the centrifugal speed increases, the centrifugal force on the core 3 increases, that is, the interaction force between the core 3 and the separation tube 2 is greater, that is, the interaction force between the wedge key groove 32 and the wedge key block 22 is greater, causing the deformation of the core 3 to increase and its internal force to increase, resulting in the fixed channel 31 squeezing the bone more tightly to improve the fixation effect, realizing the function of adaptively adjusting the bone fixation force based on the centrifugal speed, thereby enhancing the firmness of bone fixation and avoiding its movement.

[0038] Compared with the conventional double-centrifuge-tube nested centrifugal separation device, the present invention adopts an assembly centrifugal separation method for bone marrow. After the core 3 and the separation tube 2 are assembled, a positive-correlation interaction force with the centrifugal speed is generated between the wedge key block 22 and the wedge key groove 32, causing the fixed channel 31 of the core 3 to have a positive-correlation deformation amount with the centrifugal speed, tightly squeezing the bone fixed inside it, and forming a liquid-carrying section 201 capable of pre-filling the flushing liquid, so as to realize the centrifugal self-adaptive fixation of the bone and the integration of flushing and centrifugal separation, effectively improving the collection rate of bone marrow and reducing the inactivation rate of bone marrow cells, and the operation is simpler.

[0039] The end of the collecting tube 1 is close to the axis of the centrifugal movement, while the bottom thereof is far away from the axis of the centrifugal movement, so the collecting tube 1 can be placed in a conventional experimental centrifuge for bone marrow separation.

[0040] Based on the above embodiment, during the deformation process, the deformation of the core 3 will be transmitted to the fixed channel 31, causing the inner wall of the fixed channel 31 to deform. Therefore, after the core 3 is pressed tightly into the separation tube 2, the fixed channel 31 can be squeezed tightly against the bone through the interaction force between the wedge-shaped key groove 32 and the wedge-shaped key block 22, so as to avoid the flushing liquid in the liquid-carrying interval 201 flowing out from the gap between the fixed channel 31 and the bone, resulting in ineffective flushing.

[0041] Of course, when the core 3 is deformed, in order to further improve the reliability and sealing of the fixing channel 31 pressing against the bone, the following preferred embodiments are provided.

[0042] like Figure 2 As shown, the number of the fixing holes 31 and the wedge-shaped key grooves 32 is consistent, and the fixing holes 31 are located between adjacent wedge-shaped key grooves 32. During centrifugation, the wedge-shaped key grooves 32 on the core 3 are squeezed toward the wedge-shaped key blocks 22 on the separation tube 2 under the action of centrifugal force, and the fixing holes 31 are squeezed under the reaction force of the wedge-shaped key blocks 22. After deformation, the fixing holes 31 are conical to strengthen the fixation of the bones.

[0043] In this embodiment, the fixing channel 31 is set between two adjacent wedge-shaped key grooves 32. After the wedge-shaped key grooves 32 are squeezed by the wedge-shaped key blocks 22, the elastic deformation is transmitted inwardly to squeeze the bones in the fixing channel 31 inward from two directions, thereby improving the effect of tight squeezing and fixing, and can also further reduce the gap or reduce the cross-sectional area of ​​the gap.

[0044] Among them, the inner wall of the fixing hole 31 is also made of elastic material. Since the size of the bones to be separated varies to a certain extent, and the specifications of the fixing hole 31 remain unchanged, the core 3 with a diameter of the fixing hole 31 slightly larger than the maximum bone diameter of the bones to be separated is used. In addition, in the separation and material selection process, the specifications of the bones to be separated are kept as small as possible, which can effectively avoid a large difference in the tightness of bone fixation. Moreover, since bones are usually non-straight, even if the diameter of the fixing hole 31 is slightly larger than the bone diameter of the bone, when the bone is inserted into the fixing hole 31, the bone deviates in the fixing hole 31 and contacts the elastic hole wall of the fixing hole 31 to generate a large travel resistance, thereby ensuring that the bone is difficult to naturally fall out of the fixing hole 31, which is convenient for assembly.

[0045] Among them, the wedge key groove 32 is squeezed by the wedge key block 22, causing the core 3 to deform. This is because the slope of the wedge key block 22 is greater than that of the wedge key groove 32, resulting in an incomplete assembly between the core 3 and the separation tube 2, that is, an incomplete fit between the wedge key groove 32 and the wedge key block 22. Therefore, in order to make the deformation of the core 3 caused by the interaction force between the wedge key groove 32 and the wedge key block 22 effectively fix the bone, the following two embodiments are provided.

[0046] Embodiment 1:

[0047] As Figure 3 、 Figure 4 shown, the wedge key groove 32 has two inner triangular surfaces 321. One side of the two inner triangular surfaces 321 close to the axis of the core 3 is butted to form an inner groove line 322. The distance between the inner groove line 322 and the axis of the core 3 gradually decreases from top to bottom;

[0048] The wedge key block 22 has two outer triangular surfaces 221. One side of the two outer triangular surfaces 221 far from the axis of the separation tube 2 is butted to form an outer edge line 222. The distance between the outer edge line 222 and the axis of the separation tube 2 gradually decreases from top to bottom;

[0049] Among them, the slope of the outer edge line 222 is greater than that of the inner groove line 322.

[0050] In this embodiment, by reducing the slope of the inner groove line 322 of the wedge key groove 32 and increasing the slope of the outer edge line 222 of the wedge key block 22, when the wedge key groove 32 moves towards the wedge key block 22, the extrusion area between the inner triangular surface 321 and the outer triangular surface 221 is gradually increased. Thus, during the deformation transfer, the deformation area of the fixing hole 31 is effectively increased, that is, the inner wall of the fixing hole 31 squeezes the bone inward in a large area, further improving the fixing effect and the suturing effect.

[0051] Embodiment 2:

[0052] As Figure 3 、 Figure 4 shown, the distance between the two inner triangular surfaces 321 gradually increases from top to bottom, the distance between the two outer triangular surfaces 221 gradually increases from top to bottom, and the increment of the distance between the two inner triangular surfaces 321 from top to bottom is less than the increment of the distance between the two outer triangular surfaces 221 from top to bottom.

[0053] In this embodiment, by reducing the increment of the distance between the two inner triangular surfaces 321 from top to bottom and increasing the increment of the distance between the two outer triangular surfaces 221 from top to bottom, when the wedge-shaped key groove 32 moves towards the wedge-shaped key block 22, the extrusion deformation distance between the inner triangular surface 321 and the outer triangular surface 221 is gradually increased. Thus, during the deformation transmission, the deformation degree of the fixed hole 31 is effectively improved, that is, the inner wall of the fixed hole 31 presses the bone inward significantly, further improving the fixing effect and the seam shrinking effect.

[0054] Both Embodiment 1 and Embodiment 2 can achieve the effects of improving the bone fixing effect and reducing the gap or reducing the gap area during the centrifugation process. Of course, the solutions of Embodiment 1 and Embodiment 2 can also be combined and applied to further improve the fixing effect and the seam shrinking effect.

[0055] Based on the above embodiments, it can be known that the separation tube 2 has a tendency to move towards the lower half of the collection tube 1 under the centrifugal force. In order to prevent the separation tube 2 from continuing to move downward and occupying the volume of the lower half of the collection tube 1 (i.e., the collection area), after the separation tube 2 is assembled on the collection tube 1, the bottom of the separation tube 2 needs to be inserted into the collection tube 1 by a fixed distance. Based on this, the following preferred embodiments are provided.

[0056] As Figure 1 shown, the outer wall diameter of the separation tube 2 is less than or equal to the inner wall diameter of the collection tube 1, and the outer edge of the tube orifice of the separation tube 2 protrudes outward and abuts against the tube orifice of the collection tube 1, so as to limit the movement of the separation tube 2 into the collection tube 1 after the separation tube 2 is subjected to the centrifugal force, and reserve a space for collecting bone marrow in the lower half of the collection tube 1.

[0057] In this embodiment, the outer edge of the tube orifice of the separation tube 2 protrudes outward with a rim whose diameter is larger than the tube orifice of the collection tube 1. After the separation tube 2 and the collection tube 1 are nested and installed, the rim abuts against the end of the tube orifice of the collection tube 1, restricting the separation tube 2 from continuing to move into the collection tube 1, so as to prevent the separation tube 2 from occupying the collection area during the centrifugation process.

[0058] In the above embodiment, the separated and rinsed bone marrow and the rinsing liquid in the bone flow out from the diversion channel 21 into the lower half of the collection tube 1. During the diversion process of the diversion channel 21, the liquid flowing out of the diversion channel 21 is likely to flow along the bottom of the separation tube 2 under the centrifugal force, resulting in part of the bone marrow cells adhering to the separation tube 2, causing waste, that is, greatly reducing the content of bone marrow cells in the collected liquid. Based on this, the following preferred embodiments are provided.

[0059] As Figure 1 shown, the bottom of the separation tube 2 converges towards the center and protrudes downward to form a nipple 23, and the diversion channel 21 runs through along the axis of the nipple 23.

[0060] In this embodiment, a nipple 23 that clusters towards the center and protrudes downward is formed at the bottom of the separation tube 2, and the diversion channel 21 runs through along the axis of the nipple 23. The end face area of the nipple 23 is set to be minimized, that is, the wall thickness of the nipple 23 is reduced, so as to effectively reduce the contact area between the liquid flowing out of the diversion channel 21 and the outside of the separation tube 2, that is, reduce the adhesion residue and increase the content of bone marrow cells in the collected liquid.

[0061] Of course, as Figure 1 shown, a limiting structure is provided at the opening of the separation tube 2, and the limiting structure maintains a fixed relative position with the collection tube 1 under the action of centrifugal force. Among them, the limiting structure is specifically a T-shaped ring with an outer edge protruding outward and abutting against the mouth of the collection tube 1. The T-shaped ring is closely attached to the collection tube 1 under the action of centrifugal force and restricts the relative movement between the separation tube 2 and the collection tube 1.

[0062] Based on the above embodiments, taking the femur / tibia of a mouse as an example, the specific implementation process of the present invention is as follows:

[0063] Step 100: Obtain sterile mouse femur / tibia;

[0064] Step 200: Open both ends of the mouse femur / tibia and place them in the fixing channel 31 of the core 3.

[0065] Step 300: Place the core 3 into the separation tube 2, insert the separation tube 2 into the collection tube 1, add an appropriate amount of liquid medium (such as induction medium, PBS, etc.) into the tube cavity formed between the core 3 and the upper half of the separation tube 2, cover the cover, and place the collection tube 1 in the centrifuge.

[0066] Step 400: Set an appropriate rotation speed and time for centrifugation.

[0067] During centrifugation, the core 3 moves towards the bottom of the separation tube 2 under the action of centrifugal force and is squeezed, then the wedge-shaped key groove 32 is squeezed inward by the wedge-shaped key block 22, so that the core 3 generates a deformation of gathering towards the center, reducing the inner diameter of the fixing channel 31 of the core 3, making the core 3 fully fit with the bone in its fixing channel 31. After deformation, the fixing channel 31 is roughly conical, enhancing the fixing effect of the device on the bone and the converging and collecting effect on tissue components and liquids.

[0068] At this time, the bone marrow and other components in the bone are converged and collected into the lower half of the collection tube 1. The liquid medium in the device is centrifuged and flushes the inside of the bone, and also converges into the lower half of the collection tube 1.

[0069] Step 500: If necessary, repeat Step 400 to improve the bone marrow cell acquisition rate.

[0070] Using the device of the present invention can save time and improve efficiency. Taking a mouse as an example, starting from the time when the bone is obtained and the separation preparation begins, the time taken by this method is about 5 minutes. At the same time, it can reduce the contact and attachment between the bone marrow and the outside world, with a high yield and a low pollution risk. Moreover, compared with the conventional centrifugation method, the separation is more thorough, and it has the advantages of both separation methods, that is, rinsing and centrifugation are integrated, and rinsing is carried out by using the centrifugal force during centrifugation, combining the advantages of centrifugal separation and rinsing separation (it takes 20 minutes to use rinsing separation alone).

[0071] Moreover, it saves consumables and has a high cost performance. Taking the extraction of BMDMs as an example, directly rinsing the bones of each mouse requires about 20 mL of induction medium, while only 1-2 mL is needed using this method. And the operation is simple and the learning cost is low.

[0072] The above embodiments are only exemplary embodiments of the present application and are not used to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art can make various modifications or equivalent replacements within the essence and protection scope of the present application, and such modifications or equivalent replacements should also be regarded as falling within the protection scope of the present application.

Claims

1. An adaptively fixed bone marrow assembly separation device, characterized in that, It includes a collection tube (1) and a separation tube (2) sleeved inside the collection tube (1). The bottom of the separation tube (2) extends downward and contracts to form a diversion channel (21). At the contraction of the separation tube (2), a core (3) made of an elastic material is clamped. The separation tube (2) and the core (3) are closed to form a liquid-carrying interval (201). Inside the core (3), there are a plurality of uniformly distributed fixing channels (31) for fixing bones. Among them, on the inner wall of the separation tube (2), there are wedge-shaped key blocks (22) arranged in a circular pattern. The outer wall of the core (3) is recessed inward to form wedge-shaped key grooves (32) corresponding to the wedge-shaped key blocks (22) to complete the assembly with the separation tube (2). The inclination angle of the wedge-shaped key grooves (32) is smaller than that of the wedge-shaped key blocks, so that the core (3) is not completely fitted into the separation tube (2) in the natural state. The liquid-carrying interval (201) is used to fill the flushing liquid. During centrifugation of the cover, under the action of centrifugal force, the flushing liquid separates and fixes the bone marrow in the bones in the fixing channels (31), and then enters the bottom of the collection tube (1) through the diversion channel (21).

2. The self - adaptive fixed bone marrow assembly separation device according to claim 1, characterized in that, The number of the fixing channels (31) is the same as that of the wedge-shaped key grooves (32), and the fixing channels (31) are located between adjacent wedge-shaped key grooves (32). During centrifugation, the wedge-shaped key grooves (32) on the core (3) are extruded towards the wedge-shaped key blocks (22) on the separation tube (2) under the action of centrifugal force, and the fixing channels (31) are extruded under the reaction force of the wedge-shaped key blocks (22). After the fixing channels (31) are deformed, they are in a conical shape to strengthen the fixation of the bones.

3. An adaptive fixed bone marrow assembly separation device according to claim 2, wherein, The wedge-shaped key groove (32) has two inner triangular surfaces (321). One side of the two inner triangular surfaces (321) close to the axis of the core (3) is butted to form an inner groove line (322). The distance between the inner groove line (322) and the axis of the core (3) gradually decreases from top to bottom. The wedge-shaped key block (22) has two outer triangular surfaces (221). One side of the two outer triangular surfaces (221) far from the axis of the separation tube (2) is butted to form an outer edge line (222). The distance between the outer edge line (222) and the axis of the separation tube (2) gradually decreases from top to bottom. Among them, the slope of the outer edge line (222) is greater than that of the inner groove line (322).

4. An adaptive fixed bone marrow assembly separation device according to claim 3, characterized in that, The distance between the two inner triangular surfaces (321) gradually increases from top to bottom, and the distance between the two outer triangular surfaces (221) gradually increases from top to bottom. And the increment of the distance between the two inner triangular surfaces (321) from top to bottom is less than the increment of the distance between the two outer triangular surfaces (221) from top to bottom.

5. An adaptive fixed bone marrow assembly separation device according to claim 2 or 3, characterized in that, As the centrifugal speed increases, the extrusion degree of the wedge-shaped key grooves (32) and the wedge-shaped key blocks under the action of centrifugal force is higher, so that the fixing channels (31) undergo greater deformation to adaptively strengthen the fixation of the bones following the centrifugal speed.

6. An adaptive fixed bone marrow assembly separation device according to claim 5, characterized in that, The inner wall of the fixing channel (31) is also made of an elastic material, and the inner diameter of the fixing channel (31) is larger than the maximum outer diameter of the bones.

7. An adaptive fixed bone marrow assembly separation device according to claim 1, characterized in that The bottom of the separation tube (2) converges towards the center and protrudes downward to form a nipple (23), and the diversion channel (21) runs through along the axis of the nipple (23).

8. An adaptive fixed bone marrow assembly separation device according to claim 1, characterized in that, A limiting structure is provided at the opening of the separation tube (2), and the limiting structure maintains a fixed relative position with the collection tube (1) under the action of centrifugal force.

9. An adaptive fixed bone marrow assembly separation device according to claim 7, characterized in that, The limiting structure is specifically a T-shaped ring with an outer edge protruding outwards and abutting against the mouth of the collection tube (1). The T-shaped ring is in close contact with the collection tube (1) under the action of centrifugal force and restricts relative movement between the separation tube (2) and the collection tube (1).

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