Venom extraction device suitable for new spiders

Abstract

The venom extraction device comprises an overall operation table, a chela supporting rod, a rotation adjusting piece, a chela oral cavity separation table, a fixing rod, a centrifugal tube placement opening and an electric stimulation mechanism, the spiders are subjected to anesthesia pretreatment and then fixed to the chela oral cavity separation table, and step feet are fixed through the fixing rod; and the spider chelys are opened to a proper size by using the chelys supporting rod and electrified to stimulate the poison gland to secrete venom. In the electrifying process, the venom is suspended at the tip of the chela limb in a droplet form, and is sucked and transferred into a centrifugal tube by using a pipette to finish collection; the venom extraction device suitable for the new spiders is specially designed according to the physiological structure characteristics of the new spiders, mechanical injuries are avoided while individual activities are effectively restrained, single-person operation is achieved, high efficiency and labor saving are achieved, the venom extraction success rate and yield can be increased, venom collection is convenient and reliable, the conductive effect is good, and electric stimulation is safely and accurately controlled; and invalid power-on and energy consumption are reduced.

Description

Technical Field

[0001] The present invention belongs to the technical field of spider venom extraction, and in particular, to a venom extraction device suitable for new spider species of Araneomorphae spiders. Background Art

[0002] Spider venom, as a natural resource rich in various proteins and bioactive peptides, has broad application prospects in fields such as drug development, neuroscience research, and agricultural pest control. With the continuous in-depth research in related fields, how to achieve efficient, stable, and safe extraction of spider venom has become a key technical problem that urgently needs to be broken through in this field. From a taxonomic perspective, spiders can be mainly divided into two major categories: Araneomorphae and Mygalomorphae. There are significant differences in their chelicerae structures and venom secretion mechanisms. Therefore, it is of great significance to develop targeted venom extraction devices based on the physiological structure characteristics of different groups of spiders. Especially for new spider species of Araneomorphae spiders, due to their large variety, complex venom components, and strong potential pharmacological activities, they have received extensive attention in toxinology research in recent years. However, the currently commonly used venom extraction methods mainly rely on traditional manual operations, such as the manual stimulation method and the forceps method. These methods have cumbersome operation processes, low extraction efficiency, and high safety risks. Usually, multiple operators are required to cooperate to complete the operation. The overall venom output is small, the stability is poor, and it is easy to cause damage to the spiders if not careful. When dealing with new spider species of Araneomorphae spiders with large body sizes and fierce temperaments (such as Heteropoda venatoria, Leptoctenus tuberculatus, etc.), the traditional venom extraction methods are difficult to meet the actual needs of high efficiency, safety, and single-operator operation, which greatly limits the progress of related research and applications.

[0003] Since the existing spider venom extraction technologies are mostly used in research laboratories, often relying on methods such as manual operation, electrical stimulation, and collecting venom with glass capillary tubes, there are problems such as low extraction efficiency, high operation difficulty, and high risk of damage to individual spiders. Especially when dealing with new spider species of Araneomorphae spiders with large body sizes or complex venom gland distributions, the traditional devices have many limitations in terms of fixation, power supply method, and operation convenience. Therefore, it is of important practical value to develop a venom extraction device specifically designed for new spider species of Araneomorphae spiders, which is safe, efficient, and has a stable structure. Summary of the Invention

[0004] Object of the Invention: To provide a venom extraction device suitable for new spider species of Araneomorphae spiders, so as to solve the problem in the prior art that there is a lack of a dedicated structural design for new spider species of Araneomorphae spiders, and it is impossible to effectively realize the coordinated operation of spider fixation, electrical stimulation, and venom collection.

[0005] Technical Solution:

[0006] A venom extraction device applicable to new spider species, comprising an overall operation table, an electric stimulation mechanism, a spider limiting mechanism, and a venom collection assembly provided on the overall operation table. The spider limiting mechanism is electrically connected to the electric stimulation mechanism. The spider limiting mechanism includes a first limiting assembly and a second limiting assembly. The first limiting assembly is used to limit the spider's legs, and the second limiting assembly is used to limit the spider's chelicerae;

[0007] The first limiting assembly includes a chelicera oral separator and a fixing rod. The fixing rod is located at one end of the chelicera separator in the length direction and is rotatably connected to the chelicera separator. The fixing rod is used to fix the spider's legs on the chelicera oral separator;

[0008] The second limiting assembly includes a rotation adjustment member and two groups of chelicera struts. The two groups of chelicera struts are provided at the other end of the chelicera oral separator in the length direction and are connected to the rotation adjustment member. The rotation adjustment member is rotatably provided on the chelicera oral separator. Rotating the rotation adjustment member drives the two groups of chelicera struts to move towards each other along the width direction of the chelicera oral separator to adjust the distance between the spider's chelicerae;

[0009] The venom collection assembly includes at least one centrifuge tube placement opening opened on the overall operation table. The centrifuge tube placement opening is used to fix the venom collection container.

[0010] In a further embodiment, the chelicera oral separator includes a central fixing table and a fixing rod. The fixing rod includes a cross bar and pressing rods vertically fixed at both ends of the cross bar. The two pressing rods are symmetrically provided on both sides of the central fixing table in the length direction. The cross bar is rotatably connected to a screw knob. The screw knob is located at one end of the central fixing table and is fixed on the overall operation table. The other end of the central fixing table is provided with the chelicera strut;

[0011] The fixing rod is a flexible pressing rod made of rubber, which plays a role in protecting the spider's legs from being damaged during fixation.

[0012] In a further embodiment, the longitudinal cross-section of the side surface of the central fixing table is triangular. The surface of the central fixing table is provided with a metal contact surface, and a negative connection port is provided on the metal contact surface. The negative connection port is connected to the electric stimulation mechanism.

[0013] In a further embodiment, the chelicera strut includes a spreading rod and a cursor card slot. The spreading rod is fixed on the cursor card slot. The cursor card slot is located in an empty slot opened in the overall operation table. One end of the spreading rod extends into the empty slot from the slot opening of the empty slot and is connected to the cursor card slot. The cursor card slot is slidably provided in the empty slot and is connected to the rotation adjustment member;

[0014] The spreading rod is made of conductive metal, and the cursor slot is provided with a positive electrode connection port, which is connected to the electric stimulation mechanism.

[0015] In a further embodiment, the rotation adjustment member includes a bearing shaft and a button cap, the extension direction of the bearing shaft is consistent with the width direction of the central fixed platform, the bearing shaft passes through the empty slot and is penetrated and rotatably connected to the integral operating table, the button cap is installed on the end of the bearing shaft located outside the integral operating table, and the shaft body of the bearing shaft located in the empty slot is provided with a thread that engages with the cursor slot.

[0016] In a further embodiment, the electric stimulation mechanism includes a switch, a control circuit and a power pedal, the power pedal is used to control the start and stop of the control circuit, the switch is arranged on the integral operating table, a voltage level adjustment button, a power level and voltage level display screen and a charging port are also arranged on the integral operating table, and the switch is used to control the start and stop of the power supply;

[0017] The control circuit includes a multi-level voltage adjustment module and a display module, wherein the multi-level voltage adjustment module is connected to the voltage level adjustment button, and the display module is connected to the power level and voltage level display screen, wherein the voltage level adjustment button is used to adjust the output voltage according to different spider species, and the power level and voltage level display screen displays the current output voltage value and power level status in real time;

[0018] The charging port is a USB interface, and the charging port is connected to a built-in battery arranged in the integral operating table.

[0019] In a further embodiment, the power pedal is a rebound structure, and the electrode is controlled to be on and off by stepping on it, freeing the operator's hands and improving operating efficiency. The power pedal shell is made of waterproof material to adapt to a variety of operating environments.

[0020] In a further embodiment, the bottom of the integral operating table is provided with a weighted structure and a non-slip silicone pad to improve the stability of the device, which is suitable for laboratory countertops or temporary outdoor operating platforms.

[0021] In a further embodiment, the target spider is placed in front of the chelicerae oral partition table for anesthetic pretreatment, the target spider is anesthetized by anesthetic drugs, and the anesthesia lasts for no less than 5 minutes. After the target spider enters a syncope, slows down movements or even stops moving after anesthesia, the anesthesia is considered to be complete.

[0022] In a further embodiment, it also includes performing a conductive auxiliary operation on the target spider placed on the chelicerae oral partition table before using the electrical stimulation mechanism, and the conductive auxiliary operation includes using pure water to soak the contact surface between the target spider's chelicerae and the chelicerae support rods.

[0023] Advantages of the present invention:

[0024] (1) High adaptability and safe fixation design: This device is specially designed according to the physiological structure characteristics of new spider species. The chelicera oral partition platform and the fixing rod are structured to match the body length and leg length of the spider. Rubber pressure rods are used to flexibly fix the legs, which can effectively restrict the individual's activities while avoiding mechanical damage and improving the animal welfare level.

[0025] (2) Single-person operation, highly efficient and labor-saving, and can improve the success rate and yield of venom extraction: The device has a compact structure, is small and convenient. All functions are concentrated in the integrated platform of the operation table. The power supply is triggered by a pedal to free the hands, and the rotation adjustment part is used to control the precise advancement of the support rod. The entire venom extraction process can be independently completed by a single person, greatly improving work efficiency, reducing the dependence on manpower. Compared with traditional stimulation methods, this device can more effectively induce venom secretion, reduce manual intervention and failure rate, and increase the amount of venom that can be obtained per unit time, meeting the needs of large-scale venom collection.

[0026] (3) Convenient and reliable venom collection: The collection container is stably fixed through the centrifuge tube placement port, and a standard pipette is used for transfer to ensure that the venom collection process is not contaminated and is also convenient for subsequent lyophilization, separation or pharmacological analysis.

[0027] (4) Good electrical conductivity and safe and precise control of electrical stimulation: The voltage levels used for electrical stimulation are all within the safe range. The on-off circuit is controlled by the power pedal. Combining the voltage level adjustment function and real-time monitoring of the display screen, the operator can adjust the stimulation intensity according to the spider's reaction to obtain the best venom secretion effect, avoiding stress reactions or death caused by over-stimulation; the electrode structure is optimized with high electrical conductivity. The chelicera support rod and the oral partition platform are both made of conductive metal materials, and the stimulation parts are all treated with pure water infiltration to effectively improve the electrical conductivity, ensure that the current is controllable and concentrated, precisely stimulate the venom gland, and reduce ineffective power-on and energy consumption.

[0028] (5) Suitable for multi-scenario use in the wild and laboratory: The built-in battery design supports long-term off-grid operation, equipped with a USB Typ-C charging interface for convenient outdoor power supply. The bottom of the overall operation table is provided with anti-slip silicone pads and weights to ensure stability during outdoor or desktop operations.

[0029] (6) Promote the research and development of new spider venom for medicinal use: This device provides a standardized operation platform for obtaining new spider venom, can provide a stable raw material source for new drug screening, extraction of bioactive components, toxicological experiments, etc., and has important scientific research and industrialization value. Brief description of the drawings

[0030] Figure 1This is a schematic structural diagram of an apparatus for extracting venom from spiders of the family Neosparassidae according to the present invention;

[0031] Figure 2 This is a front view of an apparatus for extracting venom from spiders of the family Neosparassidae according to the present invention;

[0032] Figure 3 This is a side view of an apparatus for extracting venom from spiders of the family Neosparassidae according to the present invention;

[0033] Figure 4 This is a top view of an apparatus for extracting venom from spiders of the family Neosparassidae according to the present invention;

[0034] Figure 5 This is a partial enlarged schematic diagram of the spider fixation and chelicerae spreading structure in the present invention;

[0035] Figure 6 This is a schematic diagram of the structure of the chelicerae support rod rotation adjustment member inside the operating table in the present invention;

[0036] Figure 7 This is a schematic diagram of the chelicerae oral cavity separation table and the fixing rod structure in the present invention;

[0037] Figure 8 This is a schematic diagram of the electrical stimulation control circuit in the present invention.

[0038] The reference signs are: overall operating table 1, chelicerae support rod 2, spreading rod 201, cursor card slot 202, positive connection port 203, rotation adjustment member 3, knob cap 301, bearing 302, chelicerae oral cavity separation table 4, central fixing table 401, metal contact surface 402, negative connection port 403, fixing rod 5, pressing rod 501, screw knob 502, centrifuge tube placement port 6, power supply pedal 7, switch 8, voltage level adjustment button 9, power quantity and voltage level display screen 10, charging port 11. Detailed implementation manners

[0039] In the following description, numerous specific details are set forth in order to provide a more thorough understanding of the present invention. However, it will be apparent to one of ordinary skill in the art that the present invention may be practiced without one or more of these details. In other instances, well-known features have not been described in order to avoid obscuring the present invention.

[0040] The present invention will be further described in detail below with reference to the accompanying drawings.

[0041] Refer to Figure 1-8, a venom extraction device for new spider spiders disclosed in the present invention, including an overall operation table 1 and an electric stimulation mechanism, a spider limiting mechanism and a venom collection component arranged on the overall operation table 1. The spider limiting mechanism is electrically connected to the electric stimulation mechanism. The spider limiting mechanism includes a first limiting component and a second limiting component. The first limiting component is used to limit the legs of the spider, and the second limiting component is used to limit the chelicerae of the spider. The first limiting component includes a chelicera oral separator 4 and a fixing rod 5. The fixing rod 5 is located at one end of the chelicera separator in the length direction and is rotatably connected to the chelicera separator. The fixing rod 5 is used to fix the legs of the spider on the chelicera oral separator 4. The second limiting component includes a rotation adjustment member 3 and two groups of chelicera struts 2. The two groups of chelicera struts 2 are arranged at the other end of the chelicera oral separator 4 in the length direction and are connected to the rotation adjustment member 3. The rotation adjustment member 3 is rotatably arranged on the chelicera oral separator 4. Rotating the rotation adjustment member 3 drives the two groups of chelicera struts 2 to move towards each other along the width direction of the chelicera oral separator 4 to adjust the distance between the chelicerae of the spider. The venom collection component includes at least one centrifuge tube placement port 6 opened on the overall operation table 1. The centrifuge tube placement port 6 is used to fix the venom collection container.

[0042] The chelicera oral separator 4 includes a central fixing table 401 and a fixing rod 5. The fixing rod 5 includes a cross bar and pressing rods 501 vertically fixed at both ends of the cross bar. The two pressing rods 501 are symmetrically arranged on both sides of the central fixing table 401 in the length direction. The cross bar is rotatably connected to a screw button 502. The screw button 502 is located at one end of the central fixing table 401 and is fixed on the overall operation table 1. The other end of the central table fixing table is provided with the chelicera strut 2. The screw button 502 has firmness and is not easy to loosen, and is used to adjust the pressing degree of the pressing rod 501 to adapt to spiders of different body types. The chelicera oral separator 4 can expose the chelicerae of the spider and separate the mouthparts, facilitating the electrodes to contact the target part during electric stimulation, and at the same time can prevent the oral fluid secreted by the spider from contaminating the venom. The fixing rod 5 is a flexible pressing rod 501 made of rubber material, which plays a role in protecting the legs of the spider from being damaged during fixation.

[0043] The longitudinal cross-section of the side surface of the central fixing table 401 is triangular. The surface of the central fixing table 401 is provided with a metal contact surface 402. The metal contact surface 402 is provided with a negative electrode connection port 403. The negative electrode connection port 403 is connected to the electric stimulation mechanism. The triangle is preferably a right triangle, and the cross bar is located at one end of the smallest acute angle of the triangle.

[0044] The chelicera strut 2 includes a spreading rod 201 and a cursor card slot 202. The spreading rod 201 is fixed on the cursor card slot 202. The cursor card slot 202 is located in an empty slot opened in the overall operating table 1. One end of the spreading rod 201 extends into the empty slot from the slot opening of the empty slot and is connected to the cursor card slot 202. The cursor card slot 202 is slidably arranged in the empty slot and is connected to the rotation adjustment member 3. To further ensure the stability of the cursor card slot 202, a slider is installed on the cursor card slot 202. The slider is slidably connected in a chute. The chute is opened on the wall of the empty slot. The cooperation of the slider and the chute not only improves the connection between the cursor card slot 202 and the empty slot, but also makes the moving directivity and stability of the cursor card slot 202 better. The spreading rod is made of conductive metal material. A positive connection port 203 is provided on the cursor card slot 202. The positive connection port 203 is connected to the electric stimulation mechanism.

[0045] The rotation adjustment member 3 includes a bearing shaft 302 and a knob cap 301. The extending direction of the bearing shaft 302 is consistent with the width direction of the central fixing table 401. The bearing shaft 302 passes through the empty slot and is rotatably connected to the overall operating table 1. The knob cap 301 is installed on the end of the bearing shaft 302 located outside the overall operating table 1. A thread that engages with the cursor card slot 202 is provided on the shaft body of the bearing shaft 302 located in the empty slot. When the bearing shaft 302 is rotated by driving the knob cap 301, the thread rotates accordingly, thereby moving the cursor card slot 202 connected therewith outward or inward in the empty slot. At this time, the spreading rod also moves along the slot opening of the empty slot. By rotating the rotation adjustment member 3, precise control of the distance between the spreading rods can be achieved. During use, the lower part of the spider sternum is closely attached to the metal contact surface 402, and its chelicerae extend forward to the front side of the central fixing table 401 and are engaged and positioned with the spreading rod 201. At the same time, the spider legs are pressed against the metal contact surface 402 by the rubber pressing rods 501 on both sides, thereby completing the fixing operation of the spider individual.

[0046] The electric stimulation mechanism includes a switch 8, a control circuit, and a power pedal 7. The power pedal 7 is used to control the start and stop of the control circuit. The switch 8 is provided on the overall operating table 1. On the overall operating table 1, there are also a voltage gear adjustment button 9, a power and voltage gear display screen 10, and a charging port 11. The switch 8 is used to control the start and stop of the power supply. One end of the power output is connected to the chelicera strut 2, and the other end is connected to the sternal contact conductive surface, thus forming a closed circuit. The voltage adjustment range is 0–10V (within the safe range), and it can be adjusted according to the physiological response characteristics of different spider species. The control circuit includes a multi-gear voltage adjustment module and a display module. The multi-gear voltage adjustment module is connected to the voltage gear adjustment button 9, and the display module is connected to the power and voltage gear display screen 10. The voltage gear adjustment button 9 is used to adjust the output voltage according to different spider species. The power and voltage gear display screen 10 displays the current output voltage value and the power status in real time. The charging port 11 is a USB interface, and the charging port 11 is connected to the built-in battery provided in the overall operating table 1, and is equipped with a USB Typ-C charging interface for convenient outdoor power supply.

[0047] The power pedal 7 has a rebound structure, and the on-off of the electrode is controlled by stepping on it, liberating the operator's hands and improving the operation efficiency. The shell of the power pedal 7 is made of waterproof material, adapting to various operating environments.

[0048] The bottom of the overall operating table 1 is provided with a weight structure and an anti-slip silica gel pad to improve the stability of the device, which is suitable for laboratory countertops or outdoor temporary operating platforms.

[0049] It also includes pre-anesthetizing the target spider placed in front of the chelicera oral separator 4. The target spider is anesthetized with an anesthetic drug, and the anesthetic duration is not less than 5 minutes. After observing that the target spider enters a fainting state, moves slowly or even remains motionless after anesthesia, it can be regarded as the completion of anesthesia. The anesthetic drug is ether or isoflurane anesthesia.

[0050] It also includes performing conductive assistance operations on the target spider placed on the chelicera oral separator 4 before using the electric stimulation mechanism. The conductive assistance operations include wetting the contact surface between the chelicera of the target spider and the chelicera strut 2 with pure water, then turning on the power device, adjusting the voltage to the lowest gear, and starting the electrical stimulation program by stepping on the power pedal 7. If the spider shows reactions such as the extension of the walking legs or palps, slight struggling, or it is already visible that venom droplets are secreted from the tip of the chelicera, it indicates that the circuit has formed a closed loop and the stimulation has taken effect successfully. At this time, gently suck the venom droplets hanging at the tip of the chelicera with a 2μL micropipette and transfer them to the venom collection container, that is, the centrifuge tube, in the centrifuge tube placement port 6 for collection and preservation. The above steps can be repeated multiple times according to experimental needs to achieve batch collection of venom.

[0051] First, the target spider needs to be pre-anesthetized. The commonly used anesthetic methods are ether or isoflurane anesthesia, with a duration of not less than 5 minutes. After observing that the spider enters a fainting state, its movements become slow or even motionless, it can be regarded as the completion of anesthesia.

[0052] After that, place the lower part of the spider's sternum on the metal contact area on the oral chelicera separation table. The surface of this area is covered with a metal conductive sheet, which serves as one contact point of the electrical stimulation circuit. On both sides of the platform, there are adjustable-angle and position walking leg fixing rods 5 for fixing the position of the spider's walking legs on the platform to prevent its struggling from affecting the experiment. In front of the separation table, there are two chelicera support rods 2, and the distance between the support rods can be adjusted according to the size of the spider individual. The chelicera distance is adjusted through a knob device to open and fix the spider's chelicera.

[0053] The electrical stimulation module includes a power supply host, a voltage adjustment gear switch 8, and a foot switch 8. One end of the power output is connected to the chelicera support rod 2, and the other end is connected to the sternum contact conductive surface, thus forming a closed circuit. The voltage adjustment range is 0–10V (within the safe range), and it can be adjusted according to the physiological response characteristics of different spider species.

[0054] Moisten the contact points between the spider's chelicera and the support rod with pure water or physiological saline to enhance conductivity. Turn on the power switch 8, adjust the voltage gear to the lowest gear, and start the electrical stimulation by stepping on the foot switch 8. The spider usually shows stress responses such as straightening of the walking legs and twitching of the palps, and venom droplets can be seen forming at the tips of the chelicera. This indicates that the electrical stimulation path is formed.

[0055] At this time, the operator uses a 2μL micropipette to collect the venom droplets, and transfers the liquid to a numbered centrifuge tube for storage. Depending on the experimental requirements, the same individual can be subjected to several electrical stimulations and venom collections, or multiple spiders can be processed batchwise. The overall structure of this device is simple and the operation is efficient. It is suitable for the standardized collection of spider venom, especially for newly discovered spider species with small body size and small venom volume, effectively improving the success rate of venom collection and the quality of samples.

[0056] Specifically, when using the venom extraction device of the present invention to extract venom from spiders, the voltage output range can be appropriately adjusted according to the size of the spiders. Among them, when larger spiders are not sensitive to lower voltage stimulation, the voltage can be appropriately increased to ensure the venom extraction effect. In practical applications, 12 adult Rhitymna verruca (Wang, 1991) spiders that were raised under the same environmental conditions, had good growth conditions, and similar body sizes were selected in this experiment. Before the experiment, they were uniformly anesthetized with ether for 6 minutes, and then electrostimulated under a voltage condition of 6.5 V. The electroshock frequency was once every 3 seconds, and the duration of each electroshock was 0.5 seconds. After each electroshock stimulation, the venom droplets secreted by the spiders were collected once. When electroshocked continuously for more than 5 times and no more venom was secreted, it was determined that the venom discharge was complete. Under these conditions, the venom was collected using the traditional manual venom extraction method and the venom extraction device of the present invention respectively. The statistical results of the venom quality obtained from venom extraction, the time required for venom extraction, the number of people participating in the collection, and the 24-hour survival rate of the spiders after venom extraction are shown in Table 1.

[0057] Protein concentration determination method: Take 5 μL of protein sample and use a BCA kit to determine the protein concentration. The method is as follows: 1) Add the standard products to the sample wells of the enzyme-linked immunosorbent assay (ELISA) strip at 0 μL, 5 μL, 10 μL, 15 μL, and 20 μL, and make up to 20 μL with sample diluent, and detect 3 replicates for each; 2) Add 5 μL of the protein sample to be tested to the sample wells of the ELISA strip, and make up to 20 μL with sample diluent, and detect 3 replicates for each; 3) Add 200 μL of BCA working solution to each well and let it stand and react at 37 °C for 30 minutes; 4) Use an ELISA reader to measure A570 (the optimal absorption wavelength is 562 nm, and other wavelengths between 540 - 595 nm can also be applied); 5) Calculate the protein concentration of the sample according to the standard curve and the volume of the sample used.

[0058] Table 1: Comparison of various data between the present invention and traditional manual spider venom collection

[0059]

[0060] The experimental results show that compared with the traditional manual venom extraction method, the venom extraction device described in the present invention has significant advantages in terms of venom extraction quality, venom protein concentration, collection efficiency, and operation convenience. The device of the present invention can collect more and higher-concentration venom in a shorter time, and the operation process only requires one person to complete, significantly reducing the labor cost. At the same time, during the 24-hour observation after venom extraction, the spider individuals collected using this device all maintained good survival status, and the survival rate was the same as that of the traditional method, indicating that the present invention will not cause adverse effects on the health of spiders while improving the venom extraction efficiency, and has good safety and practicability, and is suitable for popularization and application.

[0061] Although embodiments of the present invention have been shown and described, those of ordinary skill in the art will appreciate that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

[0062] The preferred embodiments of the present invention have been described in detail above in conjunction with the accompanying drawings. However, the present invention is not limited to the specific details in the above embodiments. Within the scope of the technical concept of the present invention, various equivalent transformations can be made to the technical solutions of the present invention, and these equivalent transformations all fall within the protection scope of the present invention.

Claims

1. A venom extraction device applicable to new spider species, characterized in that: It comprises an integral operating table and an electric stimulation mechanism, a spider limiting mechanism and a venom collection component arranged on the integral operating table, wherein the spider limiting mechanism is electrically connected to the electric stimulation mechanism, and the spider limiting mechanism comprises a first limiting component and a second limiting component, wherein the first limiting component is used for limiting the walking legs of the spider, and the second limiting component is used for limiting the chelicerae of the spider; The first limiting assembly includes a chelicerae oral cavity partition platform and a fixing rod, wherein the fixing rod is located at one end of the chelicerae oral cavity partition platform in the length direction and is rotatably connected to the chelicerae oral cavity partition platform, and the fixing rod is used to fix the spider's walking legs on the chelicerae oral cavity partition platform; The second limit assembly includes a rotating adjustment member and two groups of chelate support rods, the two groups of chelate support rods are arranged at the other end of the chelate oral cavity partition platform in the length direction and are connected to the rotating adjustment member, the rotating adjustment member is rotatably arranged on the chelate oral cavity partition platform, and the rotating adjustment member is rotated to drive the two groups of chelate support rods to move toward each other along the width direction of the chelate oral cavity partition platform to adjust the spacing between the spider chelates; The venom collection assembly comprises at least one centrifuge tube placement opening opened on the integral operating table, and the centrifuge tube placement opening is used to fix the venom collection container.

2. The venom extraction device for new spider species according to claim 1, wherein: The chelate oral partition platform comprises a central fixed platform and a fixed rod, wherein the fixed rod comprises a cross bar and pressure rods vertically fixed at both ends of the cross bar, and the two pressure rods are symmetrically arranged on both sides of the length direction of the central fixed platform, the cross bar is rotatably connected with a screw button, the screw button is located at one end of the central fixed platform and fixed on the integral operating platform, and the other end of the central platform fixed platform is provided with the chelate support rod; The fixing rod is a flexible pressure rod made of rubber material.

3. The venom extraction device for new spider species according to claim 2, characterized in that: The side longitudinal section of the central fixing platform is triangular in shape. The surface of the central fixing platform is provided with a metal contact surface. The metal contact surface is provided with a negative electrode connection port, and the negative electrode connection port is connected to the power stimulation mechanism.

4. A venom extraction device applicable to spiders of the family Neosittidae according to claim 1, characterized in that: The chelicerae support rod comprises a spreading rod and a cursor slot, wherein the spreading rod is fixed to the cursor slot, the cursor slot is located in an empty slot provided in the integral operating table, one end of the spreading rod extends from the notch of the empty slot into the empty slot and is connected to the cursor slot, and the cursor slot is slidably disposed in the empty slot and is connected to the rotating adjusting member; The spreading rod is made of conductive metal, and the cursor slot is provided with a positive electrode connection port, which is connected to the electric stimulation mechanism.

5. The venom extraction device for new spider species according to claim 4, characterized in that: The rotating adjustment member includes a bearing shaft and a button cap. The extension direction of the bearing shaft is consistent with the width direction of the central fixed platform. The bearing shaft passes through the empty slot and is rotatably connected to the integral operating table. The button cap is installed on the end of the bearing shaft located outside the integral operating table. The shaft body of the bearing shaft located in the empty slot is provided with a thread that engages with the cursor slot.

6. The venom extraction device for new spider species according to claim 1, characterized in that: The electric stimulation mechanism includes a switch, a control circuit and a power pedal. The power pedal is used to control the start and stop of the control circuit. The switch is arranged on the overall operation platform. On the overall operation platform, there are also voltage level adjustment buttons, a power and voltage level display screen and a charging port. The switch is used to control the start and stop of the power supply; The control circuit includes a multi-level voltage adjustment module and a display module. The multi-level voltage adjustment module is connected to the voltage level adjustment buttons. The display module is connected to the power and voltage level display screen. The voltage level adjustment buttons are used to adjust the output voltage according to different spider species. The power and voltage level display screen real-time displays the current output voltage value and the power status; The charging port is a USB interface, and the charging port is connected to an internal battery arranged inside the overall operation platform.

7. The venom extraction device for neo-aranid spiders according to claim 6, wherein: The power pedal is of a rebound structure.

8. A venom extraction device for spiders of the family Neosparassidae according to claim 1, characterized in that: The bottom of the overall operation platform is provided with a weight structure and an anti-slip silica gel pad.

9. The venom extraction device for new spider species according to claim 1, characterized in that: It also includes anesthetizing and preprocessing the target spider placed in front of the chelicera oral cavity separator. The target spider is anesthetized by an anesthetic drug, and the anesthetizing duration is not less than 5 minutes. After observing that the target spider enters a fainting state, has slow movements or even stops moving after being anesthetized, it can be regarded as the completion of anesthesia.

10. A venom extraction device for spiders of the family Neosittidae according to claim 1, characterized in that: It also includes performing a conductive auxiliary operation on the target spider placed on the chelicera oral cavity separator before using the electric stimulation mechanism. The conductive auxiliary operation includes using pure water to moisten the contact surface between the chelicera of the target spider and the chelicera support rod.

Images