Composition for inhibiting sprout tillers of plants, preparation, preparation method and application

Abstract

The invention relates to the technical field of plant cultivation, in particular to a composition for inhibiting plant sprout tillers, a preparation, a preparation method and application. The composition comprises the following active components: mepiquat chloride, methoxybenzoic acid and a silicon dioxide / cellulose gel material in a mass ratio of (8-20): (12-30): (50-150). According to the method, mepiquat chloride, methoxybenzoic acid and a silicon dioxide / cellulose gel material are combined for use, so that plant endogenous signal interference and physical blocking have a synergistic effect, multiple inhibition mechanisms are established, plant sprout tillers can be obviously inhibited, the operation is simple, the labor cost is saved, and the safety is high.

Description

Technical Field

[0001] The present invention relates to the technical field of plant cultivation, and specifically to a composition, a preparation, a preparation method and an application for inhibiting plant tillering. Background Art

[0002] During the growth process of plants, tillering refers to the physiological phenomenon that new buds germinate from parts such as the root collar, root system or stem base of the plant and form branches, which is common in perennial plants such as arbors and shrubs. Although plant tillering is helpful for natural reproduction and renewal, it may bring various negative impacts in agricultural and forestry production and horticultural management. First of all, tillering will consume a large amount of nutrients and water of the plant, affecting plant growth. Secondly, excessive tillering easily causes the canopy of the plant to be closed, reducing the ventilation and light transmission, increasing the risk of pest and disease breeding. In addition, in the landscape, disordered tillering will damage the beauty of the tree shape and increase the cost of pruning and maintenance. Therefore, reasonable regulation of tillering is crucial for plant health management and sustainable production.

[0003] The existing technologies for inhibiting plant tillering mainly include comprehensive measures such as physical pruning, chemical regulation and cultivation management. Physical pruning is mainly to timely cut off the tillering branches generated at the base of the main trunk or the root collar during the growth process of the plant to reduce nutrient dispersion and promote the thickening of the main trunk. However, this method has high labor costs, low efficiency and safety risks. Chemical regulation mainly uses plant growth regulators for regulation, but it requires multiple applications, the operation is cumbersome and the drug effect is limited. The cultivation management method is to prevent excessive tillering caused by over-vigorous root systems by reasonably regulating water and fertilizer. However, if the water and fertilizer management is slightly improper, tillering will be aggravated, and it is difficult to accurately balance in actual operation.

[0004] Therefore, it is of great significance to explore a technology with simple operation, high safety and excellent inhibitory effect on plant tillering. Summary of the Invention

[0005] In order to solve the defects existing in the prior art, the present invention provides a composition, a preparation, a preparation method and an application for inhibiting plant tillering. The present invention combines mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material, so that the interference of plant endogenous signals and physical blockage act synergistically to establish a multiple inhibition mechanism, which can significantly inhibit plant tillering, with simple operation, saving labor costs and high safety.

[0006] To achieve the above object, the present invention adopts the following technical solutions:

[0007] In the first aspect, the present invention provides a composition for inhibiting plant tillering, comprising the following active ingredients: mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material, and their mass ratio is (8-20):(12-30):(50-150).

[0008] Preferably, the mass ratio of the mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is (8-18):(12-25):(50-120).

[0009] Preferably, the mass ratio of the mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is (12-18):(15-25):(75-120).

[0010] Preferably, the mass ratio of the mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is (15-18):(20-25):(100-120).

[0011] Preferably, the mass ratio of SiO2 to cellulose in the silica / cellulose gel material is (0.01-0.1):1.

[0012] Preferably, the BET specific surface area of the silica / cellulose gel material > 200m 2 / g.

[0013] In a second aspect, the present invention provides a preparation for inhibiting plant tillering, comprising the above composition and excipients permitted in pesticides.

[0014] Preferably, the mass-volume concentration of the composition in the preparation is 4-13.5 g / L.

[0015] Preferably, the excipients include any one or several of solvents, auxiliaries, wetting agents, dispersants, antifreezing agents, thickeners, disintegrants, fillers, nutrient elements.

[0016] Preferably, the dosage form of the preparation is any one of emulsifiable concentrates, microemulsions, aqueous emulsions, suspensions, wettable powders or water dispersible granules.

[0017] In a third aspect, the present invention provides a preparation method of a composition for inhibiting plant tillering, mixing the active ingredients of the composition and then adding excipients permitted in pesticides.

[0018] Preferably, the preparation method is: mixing the components of the composition in a mixed solvent, and the mixed solvent is propylene glycol and water.

[0019] In a fourth aspect, the present invention provides an application of the composition in inhibiting plant tillering.

[0020] Preferably, the application dosage of the composition is 24-60 g / acre of mepiquat chloride, 36-90 g / acre of methoxybenzoic acid, and 150-450 g / acre of silica / cellulose gel material.

[0021] Preferably, the application rate of the composition in the application is 24 - 54 g / mu of mepiquat chloride, 36 - 75 g / mu of methoxybenzoic acid, and 150 - 360 g / mu of silica / cellulose gel material.

[0022] Preferably, the application rate of the composition in the application is 36 - 54 g / mu of mepiquat chloride, 45 - 75 g / mu of methoxybenzoic acid, and 225 - 360 g / mu of silica / cellulose gel material.

[0023] Preferably, the application rate of the composition in the application is 45 - 54 g / mu of mepiquat chloride, 60 - 75 g / mu of methoxybenzoic acid, and 300 - 360 g / mu of silica / cellulose gel material.

[0024] Preferably, the application period of the composition in the application is before the germination of new plant buds.

[0025] Preferably, the application site of the composition is the plant branches.

[0026] Preferably, the application is to spray the composition once a year.

[0027] Preferably, the plant is Lagerstroemia indica or Osmanthus fragrans.

[0028] The beneficial effects of the present invention are:

[0029] The composition for inhibiting plant tillering of the present invention, mepiquat chloride can competitively bind to the GID1 receptor, block the gibberellic acid signal transduction, resulting in the inability of DELLA protein to be degraded, thereby continuously inhibiting plant growth and tillering; methoxybenzoic acid activates the SA signal pathway, induces the up-regulation of the expression of DRM1 and SVP-related dormancy genes in the plant itself by 3.2 times, and inhibits the tillering of the plant trunk; the silica / cellulose gel material will form a breathable microporous membrane on the surface of the branches, and the oxygen permeability of the membrane layer is maintained > 15 cm 3 / (m 2 ·day), while reducing the water vapor transmission rate to < 5 g / (m 2 ·day), directly affecting the stomata to transport water and nutrients from the roots to the plant branches, resulting in the inability to meet the water and nutrients required for branch tillering, and further inhibiting the tillering of plant branches through physical barrier. The three active components act synergistically to achieve efficient inhibition of plant tillering from three levels: hormone regulation, dormancy gene induction, and physical barrier.

[0030] The preparation method of the composition for inhibiting plant tillering of the present invention is simple, ready to use immediately, and significantly improves the working efficiency of garden management and protection.

[0031] When the composition of the present invention is applied to inhibit plant tillering, it only needs to be sprayed on the trunk once a year to achieve an efficient tillering inhibition effect. The operation is simple, the safety is high, and the labor cost is saved. Detailed implementation mode

[0032] In order to enable those skilled in the art to better understand the technical solutions of the invention, the present invention will be further described in detail below in conjunction with the specific implementation modes.

[0033] In order to provide a technical solution with simple operation, high safety and excellent inhibition effect on plant tillering, the present invention first provides a composition for inhibiting plant tillering, which includes the following active ingredients: mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material, and their mass ratio is (8-20):(12-30):(50-150).

[0034] Mepiquat chloride: Also known as regulator, pix, mepiquat, auxin, methylpiperidine, methylpiperazinium, cotton strengthening agent; Chemical name: 1,1-dimethylazacyclohexyl chloride, 1,1-dimethylpiperidinium chloride, N,N-dimethylpiperidine chloride, N,N-dimethylpiperidinium chloride; Molecular formula C7H 16 ClN.

[0035] Methoxybenzoic acid: 4-methoxybenzoic acid, chemical molecular formula CH3OC6H4CO2H, molecular weight is 152.15.

[0036] The silica / cellulose gel material of the present invention can be directly obtained commercially or can be prepared by conventional preparation methods such as in-situ polymerization method, blending method, sol-gel method, impregnation method. In some embodiments of the present invention, the blending method is used to prepare the silica / cellulose gel material. The blending method is to mix silica powder or sol with cellulose material to make it evenly dispersed, and then form a gel through appropriate treatment. Specifically as follows:

[0037] 1. Dissolve cellulose: Place the NaOH / urea aqueous solution (7wt% NaOH + 12wt% urea) in a -12°C refrigerator and freeze it until it becomes a transparent gel; add cellulose (concentration 4-8wt%), and mechanically stir (500-1000 rpm) for 2-4 h until a transparent viscous solution is formed; keep the temperature ≤5°C (ice water bath) throughout the process to prevent cellulose degradation.

[0038] 2. Dispersed nano-SiO₂: Commercial dispersion can be directly added, or SiO₂ sol can be prepared by in-situ hydrolysis of TEOS. Direct addition of commercial dispersion: The SiO₂ dispersion is ultrasonically treated (power 300W, frequency 40kHz) for 30 min to avoid agglomeration; then 1-3 wt% modifier is added and stirred at 60 °C for 2 h to enhance the compatibility with cellulose. Preparation of SiO₂ sol by in-situ hydrolysis of TEOS: Calculate the amount of TEOS according to the target content of SiO₂ (5-20 wt%), dissolve it in ethanol (TEOS:ethanol = 1:4); add the TEOS ethanol solution dropwise to the cellulose solution, and at the same time add NH₃·H₂O to adjust the pH to 8-10 (alkaline hydrolysis), and stir for 4-6 h to form SiO₂ sol.

[0039] 3. Blending and dispersion: The above-mentioned SiO₂ dispersion or sol is added dropwise to the cellulose solution, and mechanically stirred (800-1200 rpm) for 2-3 h, where the mass ratio of pure SiO₂ to cellulose is (0.01-0.1):1; Ultrasonic-assisted dispersion: Use a probe sonicator (power 200-400W, pulse mode) to process for 20-30 min to ensure uniform dispersion of nanoparticles; Control the temperature at 25-40 °C throughout the process to avoid solvent evaporation or cellulose denaturation caused by high temperature.

[0040] 4. Molding and regeneration: Pour the blended and dispersed solution into a polytetrafluoroethylene (PTFE) mold; Immerse it in a 5 wt% Na₂SO₄ aqueous solution or ethanol for 1-2 h to displace the solvent to form a gel film; Rinse with deionized water until neutral to remove the residual solvent.

[0041] 5. Drying and post-treatment: Vacuum drying: 50-60 °C, vacuum degree -0.08 MPa, dry for 12-24 h.

[0042] Through the above refined control, a silica / cellulose gel material with uniform dispersion and excellent performance and a particle size of 80-150 nm can be prepared. The silica / cellulose gel materials prepared within the above parameter range can all meet the requirements of the present invention and achieve the same technical effects.

[0043] The active ingredient in the composition described in the present invention can be compounded by commercially purchasing the active ingredient, or by directly compounding a preparation containing the active ingredient.

[0044] Among the active ingredients of the present invention, mepiquat chloride and methoxybenzoic acid belong to plant endogenous signal interference components, and the silica / cellulose gel material is a physical blocker. Mepiquat chloride competitively binds to the GID1 receptor, blocking the signal transduction of gibberellic acid (GA), preventing the degradation of DELLA proteins, thereby continuously inhibiting plant growth and suppressing plant tillering; methoxybenzoic acid activates the salicylic acid (SA) signaling pathway, significantly upregulating the expression of dormancy-related genes such as DRM1 and SVP (3.2-fold), further inhibiting trunk tillering; in addition, the silica / cellulose gel material can form a breathable microporous membrane on the branch surface, with an oxygen permeability rate maintained above 15 cm 3 / (m 2 ·day), while controlling the water vapor transmission rate below 5 g / (m 2 ·day), effectively restricting the transport of water and nutrients, so that the tillering tissue is inhibited due to insufficient nutrient supply. The synergistic effect of the three components realizes the efficient inhibition of plant tillering from three aspects: hormone regulation, dormancy gene induction, and physical barrier.

[0045] In some embodiments of the present invention, the mass ratio of mepiquat chloride, methoxybenzoic acid, and the silica / cellulose gel material is any one or a value between any two of 8:12:50, 12:15:75, 15:20:100, 18:25:120, 20:30:150. Preferably, the mass ratio of mepiquat chloride, methoxybenzoic acid, and the silica / cellulose gel material is (8-18):(12-25):(50-120). Preferably, the mass ratio of mepiquat chloride, methoxybenzoic acid, and the silica / cellulose gel material is (12-18):(15-25):(75-120). Preferably, the mass ratio of mepiquat chloride, methoxybenzoic acid, and the silica / cellulose gel material is (15-18):(20-25):(100-120).

[0046] The BET specific surface area of the silica / cellulose gel material of the present invention is >200 m 2 / g. The nanocomposite with a high specific surface area can adsorb more drug molecules and enhance the drug efficacy.

[0047] The present invention also provides a preparation for inhibiting plant tillering, comprising the above composition and excipients permitted in pesticides.

[0048] The mass-volume concentration of the composition in the preparation of the present invention is 4 to 13.5 g / L. Preferably, the mass-volume concentration of the composition in the preparation is 4.6 to 13.3 g / L. Preferably, the mass-volume concentration of the composition in the preparation is 4.6 to 11 g / L. In some embodiments of the present invention, the mass-volume concentration of the composition is any one or a value between any two of 4.66 g / L, 6.8 g / L, 9 g / L, 10.86 g / L, and 13.3 g / L. As the mass-volume concentration of the composition increases, the effect of inhibiting plant tillering gradually enhances. However, after reaching a certain concentration, the effect advantage will decline. Therefore, considering the comprehensive effect and cost, most preferably, the mass-volume concentration of the composition in the preparation is 9 g / L.

[0049] The excipients of the present invention include any one or several of solvents, auxiliaries, wetting agents, dispersants, antifreezing agents, thickeners, disintegrants, fillers, and nutrient elements.

[0050] The dosage form of the preparation of the present invention is any one of emulsifiable concentrates, microemulsions, water emulsion concentrates, suspensions, wettable powders, or water dispersible granules.

[0051] The present invention also provides a preparation method of a composition for inhibiting plant tillering, in which the components of the composition are mixed and then excipients permitted in pesticides are added.

[0052] In some embodiments of the present invention, the preparation method of the present invention is: the components of the composition are mixed in a mixed solvent, and the mixed solvent is propylene glycol and water. The volume ratio of propylene glycol to water is 1:4.

[0053] The preparation method of the composition for inhibiting plant tillering of the present invention is simple, ready for use immediately after preparation, and significantly improves the working efficiency of garden management and protection.

[0054] The present invention also provides an application of the composition in inhibiting plant tillering.

[0055] In some embodiments of the present invention, the application rate of mepiquat chloride in the composition is any value among 24 g / mu, 36 g / mu, 45 g / mu, 54 g / mu, 60 g / mu or a value between any two of them; the application rate of methoxybenzoic acid is any value among 36 g / mu, 45 g / mu, 60 g / mu, 75 g / mu, 90 g / mu or a value between any two of them; the application rate of silica / cellulose gel material is any value among 150 g / mu, 225 g / mu, 300 g / mu, 360 g / mu, 450 g / mu or a value between any two of them. Preferably, the application rate of the composition in the application is 24 - 60 g / mu of mepiquat chloride, 36 - 90 g / mu of methoxybenzoic acid, and 150 - 450 g / mu of silica / cellulose gel material. Preferably, the application rate of the composition in the application is 24 - 54 g / mu of mepiquat chloride, 36 - 75 g / mu of methoxybenzoic acid, and 150 - 360 g / mu of silica / cellulose gel material. Preferably, the application rate of the composition in the application is 36 - 54 g / mu of mepiquat chloride, 45 - 75 g / mu of methoxybenzoic acid, and 225 - 360 g / mu of silica / cellulose gel material. Preferably, the application rate of the composition in the application is 45 - 54 g / mu of mepiquat chloride, 60 - 75 g / mu of methoxybenzoic acid, and 300 - 360 g / mu of silica / cellulose gel material.

[0056] In the application of the present invention, the application period of the composition is before the germination of new plant buds.

[0057] The application site of the composition of the present invention is the branches that do not need to germinate.

[0058] The composition of the present invention only needs to be sprayed once a year, without the need for multiple managements, is convenient to operate, and has a simple process.

[0059] The plants to which the present invention is applied are tree species with easily sprouting branches such as Lagerstroemia indica or Osmanthus fragrans. For example, Lagerstroemia indica in the embodiments of the present invention has a very strong sprouting ability. Whether it is from the base (root collar) or the latent buds on the main trunk, new branches may germinate. After pruning or damage, a large number of sprouts are extremely likely to be produced, and even may excessively consume nutrients, affecting the growth and flowering of the main trunk. After using the composition of the present invention, the inhibition rate is up to more than 80%, and the highest can reach 97.9%.

[0060] The above is a detailed description of the technical solution of the present invention. The following are the embodiments of the present invention.

[0061] The preparation method of the silica / cellulose gel material used in the following embodiments of the present invention is:

[0062] (1) Dissolve cellulose: Place the NaOH / urea aqueous solution (7 wt% NaOH + 12 wt% urea) in a -12°C refrigerator and freeze it until it becomes a transparent gel; then add 4 wt% cellulose (microcrystalline cellulose MCC, particle size 20 - 100 μm), and mechanically stir (500 - 1000 rpm) for 2 - 4 h until a transparent viscous solution is formed; maintain the temperature ≤ 5°C throughout the process to prevent cellulose degradation.

[0063] (2) Disperse nano-SiO₂: Ultrasonically treat the SiO₂ dispersion (particle size 10 - 50 nm) (power 300 W, frequency 40 kHz) for 30 min to avoid agglomeration; then add 1 wt% silane coupling agent KH550 and stir at 60°C for 2 h to enhance the compatibility with cellulose.

[0064] (3) Blend and disperse: Dropwise add the SiO₂ dispersion obtained in step (2) into the cellulose solution, and mechanically stir (800 - 1200 rpm) for 2 - 3 h, where the mass ratio of pure SiO₂ to cellulose is 0.01:1. Then use a probe sonicator (power 200 - 400 W, pulse mode) for ultrasonic-assisted dispersion treatment for 20 - 30 min to ensure uniform dispersion of nanoparticles; control the temperature at 25 - 40°C throughout the process to avoid solvent evaporation or cellulose denaturation caused by high temperature.

[0065] (4) Molding and regeneration: Pour the solution dispersed in step (3) into a polytetrafluoroethylene (PTFE) mold; immerse it in a 5 wt% Na₂SO₄ aqueous solution for 1 - 2 h to displace the solvent and form a gel film; rinse it with deionized water until it is neutral to remove the residual solvent.

[0066] (5) Drying and post-treatment: Vacuum drying: at 50 - 60°C, vacuum degree -0.08 MPa, dry for 12 - 24 h.

[0067] Example 1

[0068] The active ingredients of the composition in this example are: the mass ratio of mepiquat chloride, methoxybenzoic acid, and silica / cellulose gel material is 8:12:50.

[0069] Preparation method: Dissolve 32 ml of 250 g / L mepiquat chloride and 12.2 g of 98% methoxybenzoic acid by mass in a mixed solvent of 3 liters of propylene glycol and 12 liters of water (volume ratio 1:4), and then add 50 g of silica / cellulose gel material (BET specific surface area > 200 m 2 / g) and mix evenly.

[0070] Select Lagerstroemia indica shaped trees with relatively consistent growth vigor, divide the shaped trees into a medicated group and a clear water control group, with 9 trees in each group, and make good marks. The medicated group is as follows: After preparing and shaking well the said composition, use a sprayer to spray the whole trunk of the shaped trees in the medicated group on the areas that do not need to germinate (without spraying the top) before the new buds of the Lagerstroemia indica shaped trees germinate (around the end of February to the first ten days of March every year), and use 45L of liquid medicine per mu (that is, the amount of active ingredient sprayed per mu is 24g of mepiquat chloride, 36g of methoxybenzoic acid, and 150g of silica / cellulose gel material), and spray only once a year. The clear water control group sprays the same amount of clear water.

[0071] On February 28, 2024, the treatment was carried out. After a large number of new buds germinated in the clear water control group, on April 13, the number of new buds and terminal buds on the branches of the Lagerstroemia indica shaped trees was statistically recorded, and the results are shown in Table 1.

[0072] Table 1 Bud germination situation of the medicated group and the control group in Example 1

[0073]

[0074] As can be seen from Table 1, 45 days after the treatment, the average number of new buds germinated on the branches in the medicated group was 16.5, and the average number of new buds germinated on the branches in the clear water control group was 83.1. The medicated group could be reduced by 80.1% compared with the clear water control group; the average number of terminal buds germinated in the medicated group was 30.7, which was basically the same as the average number of terminal buds germinated in the clear water control group, which was 30.4. It shows that after the composition of this example is used, it can effectively inhibit the germination of new buds on the branches of Lagerstroemia indica shaped trees, reduce the consumption of nutrients in the Lagerstroemia indica tree body by the germination of new buds, and at the same time has no effect on the normal germination of the terminal buds of Lagerstroemia indica, saving a large amount of labor costs for preventing and removing suckers of Lagerstroemia indica shaped trees.

[0075] Example 2

[0076] The active ingredients of the composition in this example are: the mass ratio of mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is 12:15:75.

[0077] Preparation method: Dissolve 48 ml of 250 g / L mepiquat chloride and 15.3 g of methoxybenzoic acid with a mass concentration of 98% in a mixed solvent of 3 liters of propylene glycol and 12 liters of water (volume ratio of 1:4), and then add 75 g of silica / cellulose gel material (BET specific surface area > 200 m 2 / g) and mix evenly.

[0078] Select Lagerstroemia indica shaped trees with relatively consistent growth vigor, divide the shaped trees into a medicated group and a clear water control group, with 9 trees in each group, and make good marks. The medicated group is as follows: After preparing and shaking the composition well, use a sprayer to spray the entire trunk of the shaped trees in the medicated group in the area where no buds need to germinate (without spraying the top) before the new buds of the Lagerstroemia indica shaped trees germinate (around the end of February to the first ten days of March every year). The liquid volume used per mu is 45L (that is, the amount of active ingredients sprayed per mu is 36g of mepiquat chloride, 45g of methoxybenzoic acid, and 225g of silica / cellulose gel material). It is only sprayed once a year. The clear water control group sprays the same amount of clear water.

[0079] Used on March 2, 2024. After a large number of new buds germinated in the clear water control group, on April 13, the number of new buds and terminal buds on the branches of the Lagerstroemia indica shaped trees were statistically recorded, and the results are shown in Table 2.

[0080] Table 2 Bud germination situation of the medicated group and the control group in Example 2

[0081]

[0082] It can be seen from Table 2 that after 42 days of treatment, the average number of new buds germinated on the branches of the medicated group is 9.77, and the average number of new buds germinated on the branches of the clear water control group is 85. The medicated treatment group can reduce by 88.5% compared with the clear water control group; the average number of terminal buds germinated in the medicated group is 28.2, which is basically the same as the average number of terminal buds germinated in the clear water control group, which is 27.8. It shows that after the composition of this example is used, it can effectively inhibit the germination of new buds on the branches of Lagerstroemia indica shaped trees, reduce the consumption of nutrients in the Lagerstroemia indica tree body by the germination of new buds, and at the same time has no effect on the normal germination of the terminal buds of Lagerstroemia indica, saving a large amount of labor costs for removing suckers of Lagerstroemia indica shaped trees.

[0083] Example 3

[0084] The active ingredients of the composition in this example are: the mass ratio of mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is 15:20:100.

[0085] Preparation method: Dissolve 60 ml of 250 g / L mepiquat chloride and 20.4 g of methoxybenzoic acid with a mass concentration of 98% in a mixed solvent of 3 liters of propylene glycol and 12 liters of water (volume ratio of 1:4), and then add 100 g of silica / cellulose gel material (BET specific surface area > 200m 2 / g) and mix evenly.

[0086] Select Lagerstroemia indica shaped trees with relatively consistent growth vigor, divide the shaped trees into a medicated group and a clear water control group, with 9 trees in each group, and make good marks. The medicated group is as follows: After the composition is prepared and shaken well, use a sprayer to spray the whole trunk of the shaped trees in the medicated group on the areas that do not need to germinate (without spraying the top) before the new buds of Lagerstroemia indica shaped trees germinate (around the end of February to the first ten days of March every year). Use 45L of liquid medicine per mu (that is, the amount of active ingredients sprayed per mu is 45g of mepiquat chloride, 60g of methoxybenzoic acid, and 300g of silica / cellulose gel material), and spray only once a year. The clear water control group sprays the same amount of clear water.

[0087] Treatment was carried out on February 27, 2024. After a large number of new buds germinated in the clear water control group, on April 13, the number of new buds and terminal buds on the branches and trunks of Lagerstroemia indica shaped trees were counted and recorded. The results are shown in Table 3.

[0088] Table 3 Bud germination situation of the medicated group and the control group in Example 3

[0089]

[0090] It can be seen from Table 3 that after 46 days of treatment, the average number of new buds germinated on the branches of the medicated group was 2.1, and the average number of new buds germinated on the branches of the clear water control group was 77.2. The medicated treatment group could reduce by 97.2% compared with the clear water control group; the average number of terminal buds germinated in the medicated group was 29.7, which was basically the same as the average number of terminal buds germinated in the clear water control group, which was 29.9. It shows that after the composition of this example is used, it can effectively inhibit the germination of new buds on the branches and trunks of Lagerstroemia indica shaped trees, reduce the consumption of nutrients in the Lagerstroemia indica tree body by the germination of new buds, and at the same time have no effect on the normal germination of the terminal buds of Lagerstroemia indica, saving a large amount of labor costs for the removal of suckers of Lagerstroemia indica shaped trees.

[0091] Example 4

[0092] The active ingredients of the composition in this example are: the mass ratio of mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is 18:25:120.

[0093] Preparation method: Dissolve 72 ml of 250 g / L mepiquat chloride and 25.5 g of methoxybenzoic acid with a mass concentration of 98% in a mixed solvent of 3 liters of propylene glycol and 12 liters of water (volume ratio of 1:4), and then add 120 g of silica / cellulose gel material (BET specific surface area > 200 m 2 / g) and mix evenly.

[0094] Select Lagerstroemia indica shaped trees with relatively consistent growth vigor, divide the shaped trees into a medicated group and a clear water control group, with 9 trees in each group, and make good marks. The medicated group is as follows: After preparing and shaking well the said composition, use a sprayer to spray the whole trunk of the shaped trees in the medicated group on the areas where no budding is required (without spraying the top) before the new buds of the Lagerstroemia indica shaped trees germinate (around the end of February to the first ten days of March every year). Use 45L of liquid medicine per mu (that is, the amount of active ingredients sprayed per mu is 54g of mepiquat chloride, 75g of methoxybenzoic acid, and 360g of silica / cellulose gel material), and spray only once a year. The clear water control group sprays the same amount of clear water.

[0095] Used on March 2, 2024. After a large number of new buds germinated in the clear water control group, on April 13, count and record the number of new buds and terminal buds on the branches of the Lagerstroemia indica shaped trees. The results are shown in Table 4.

[0096] Table 4 Bud germination situation of the medicated group and the control group in Example 4

[0097]

[0098] It can be seen from Table 4 that after 42 days of treatment, the average number of new buds germinated on the branches of the medicated group is 1.67, and the average number of new buds germinated on the branches of the clear water control group is 81. The medicated group can reduce by 97.9% compared with the clear water control group; the average number of terminal buds germinated in the medicated group is 31.3, which is basically the same as the average number of terminal buds germinated in the clear water control group, which is 31.8. This shows that after the composition of this example is used, it can effectively inhibit the germination of new buds on the branches of Lagerstroemia indica shaped trees, reduce the consumption of nutrients in the Lagerstroemia indica tree body by the germination of new buds, and at the same time has no impact on the normal germination of the terminal buds of Lagerstroemia indica, saving a large amount of labor costs for the prevention and control of suckers on Lagerstroemia indica shaped trees.

[0099] Example 5

[0100] The active ingredients of the composition in this example are: the mass ratio of mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material is 20:30:150.

[0101] Preparation method: Dissolve 80 ml of 250 g / L mepiquat chloride and 30.61 g of 98% methoxybenzoic acid by mass concentration in a mixed solvent of 3 liters of propylene glycol and 12 liters of water (volume ratio 1:4), and then add 150 g of silica / cellulose gel material (BET specific surface area > 200 m 2 / g) and mix evenly.

[0102] Select Lagerstroemia indica shaped trees with relatively consistent growth vigor, divide the shaped trees into a medication group and a clear water control group, with 9 trees in each group, and make good marks. The medication group is as follows: After the composition is prepared and shaken well, use a sprayer to spray the entire trunk of the shaped trees in the medication group in the area where no buds need to germinate (without spraying the top) before the new buds of the Lagerstroemia indica shaped trees germinate (around the end of February to the first ten days of March every year). Use 45 L of liquid medicine per mu (that is, the amount of active ingredients sprayed per mu is 60 g of mepiquat chloride, 90 g of methoxybenzoic acid, and 450 g of silica / cellulose gel material), and only spray once a year. The clear water control group sprays the same amount of clear water.

[0103] Used on March 2, 2024. After a large number of new buds germinated in the clear water control group, on April 13, count and record the number of new buds and terminal buds on the branches and trunks of the Lagerstroemia indica shaped trees. The results are shown in Table 5.

[0104] Table 5 Bud germination situation of the medication group and the control group in Example 5

[0105]

[0106] As can be seen from Table 5, 42 days after treatment, the average number of new buds germinated on the branches of the medication group was 1.7, and the average number of new buds germinated on the branches of the clear water control group was 79.2. The medication group could reduce by 97.8% compared with the clear water control group; the average number of terminal buds germinated in the medication group was 12.4, which was reduced by 59.5% compared with the average number of terminal buds germinated in the clear water control group, which was 30.6. This shows that the composition of this example can effectively inhibit the germination of new buds on the branches of Lagerstroemia indica shaped trees, reduce the consumption of nutrients in the Lagerstroemia indica tree body by the germination of new buds, and also inhibit the germination of terminal buds.

[0107] Comparative Example 1

[0108] Same as Example 3, the only difference is that: the composition does not contain mepiquat chloride.

[0109] The spraying results of the composition are shown in Table 6.

[0110] Table 6 Bud germination situation of the medication group and the control group in Comparative Example 1

[0111]

[0112] As can be seen from Table 6, if the composition does not contain mepiquat chloride, the average number of new buds germinated on the branches of the medication group is only reduced by 39.8% compared with the control group, indicating that relying solely on methoxybenzoic acid and silica / cellulose gel material cannot achieve the effect of effectively inhibiting the sprouting of Lagerstroemia indica branches.

[0113] Comparative Example 2

[0114] Same as Example 3, the only difference is that: the composition does not contain methoxybenzoic acid.

[0115] The spraying results of the composition are shown in Table 7.

[0116] Table 7 Sprouting situation of new buds in the medicated group and the control group of Comparative Example 2

[0117]

[0118] As can be seen from Table 7, when there is no methoxybenzoic acid in the composition, the number of new buds germinated on the Lagerstroemia indica branches in the medicated group decreased by 59.7% compared with the control group, indicating that it is impossible to effectively inhibit the sprouting of Lagerstroemia indica branches only by using mepiquat chloride and silica / cellulose gel material.

[0119] Comparative Example 3

[0120] Same as Example 3, the difference is only that: there is no silica / cellulose gel material in the composition.

[0121] The spraying results of the composition are shown in Table 8.

[0122] Table 8 Sprouting situation of new buds in the medicated group and the control group of Comparative Example 3

[0123]

[0124] As can be seen from Table 8, when there is no silica / cellulose gel material in the composition, the number of new buds germinated on the Lagerstroemia indica branches in the medicated group decreased by 66.5% compared with the control group, indicating that it is impossible to effectively inhibit the sprouting of Lagerstroemia indica branches only by using mepiquat chloride and methoxybenzoic acid.

[0125] Based on the data of the above examples and comparative examples, it can be found that after the mass ratio of mepiquat chloride, methoxybenzoic acid, and silica / cellulose gel material is increased from 8:12:50 to 18:25:120, the sprouting rate of plant branches is increased from a reduction of 80.1% to a reduction of 97.9%, and the improvement effect is very significant. Continuing to increase the mass ratio of mepiquat chloride, methoxybenzoic acid, and silica / cellulose gel material to 20:30:150, the inhibition rate of plant branch sprouting is still relatively high, and at the same time, the number of newly germinated apical buds of the plant decreases sharply, by 59.5%.

[0126] The above is only the preferred implementation mode of the present invention. It should be noted that the above preferred implementation mode should not be regarded as a limitation of the present invention. The protection scope of the present invention should be subject to the scope defined by the claims. For those of ordinary skill in the art of this technology, without departing from the spirit and scope of the present invention, several improvements and retouches can still be made, and these improvements and retouches should also be regarded as the protection scope of the present invention.

Claims

1. A composition for inhibiting plant tillering, characterized in that, It comprises the following active ingredients: mepiquat chloride, methoxybenzoic acid and silica / cellulose gel material, and the mass ratio thereof is (8-20):(12-30):(50-150).

2. A preparation for inhibiting plant tillering, characterized in that, It comprises the composition described in claim 1 and excipients permitted in pesticides.

3. The preparation according to claim 2, wherein The mass-volume concentration of the composition in the preparation is 4-13.5 g / L.

4. A method for preparing a composition for suppressing plant tillering, characterized in that, After mixing the active ingredients of the composition described in claim 1, excipients permitted in pesticides are added.

5. Application of the composition described in claim 1 or the preparation described in claim 2 or 3 in inhibiting plant tillering.

6. The application according to claim 5, wherein In the said application, the application rates of the composition are 24-60 g / acre of mepiquat chloride, 36-90 g / acre of methoxybenzoic acid and 150-450 g / acre of silica / cellulose gel material.

7. The application according to claim 5 or 6, characterized in that, In the said application, the application period of the composition is before the germination of new plant buds.

8. The application according to claim 5 or 6, characterized in that, The application site of the composition is the plant branches.

9. The application according to claim 5 or 6, characterized in that, The said application is to spray the composition once a year.

10. The application according to claim 5 or 6, characterized in that, The said plant is Lagerstroemia indica or Osmanthus fragrans.

Images