An acne-removing and whitening composition and its application

Abstract

This invention relates to the field of cosmetic technology, and more particularly to an acne-removing and whitening composition and its application. Salicylic acid and azelaic acid have certain irritant properties. This invention innovatively incorporates azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol into a plant fermented oil, achieving a high-ratio blend that reduces the irritation of these three active ingredients to the epidermis. It rapidly improves the skin's tolerance to azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol, allowing them to better exert their effects in removing acne, fading acne scars, and whitening and removing blemishes in the dermis layer. The acne-removing and whitening composition provided by this invention is used in the preparation of cosmetics and is suitable for sensitive skin and oily acne-prone skin, providing precise and gentle solutions for skin problems such as acne and roughness.

Description

Technical Field

[0001] This invention relates to the field of cosmetic technology, and in particular to an acne-removing and whitening composition and its application. Background Technology

[0002] In the skincare industry, with the continued rise in demand for acne-fighting skincare products, there is a growing market demand for products that combine high efficacy with gentleness. Whitening and acne treatment have always been popular and sought-after needs. Salicylic acid and azelaic acid, as two highly effective skincare ingredients, each excel in whitening and acne treatment. Salicylic acid can penetrate deep into pores, effectively dissolving oil and removing dead skin cells, providing good treatment for acne and blackheads, and also possesses certain anti-inflammatory properties. Azelaic acid not only inhibits the growth of Propionibacterium acnes, reducing acne breakouts, but also improves pigmentation by inhibiting tyrosinase activity, achieving a whitening effect.

[0003] However, the current application of salicylic acid and azelaic acid in skincare products faces numerous challenges. Firstly, their differing solubilities lead to poor compatibility and stability. Salicylic acid is lipid-soluble and poorly soluble in water, while azelaic acid, due to its high melting point (105–106°C) and low water solubility (2.4 g / L), easily crystallizes in conventional formulations. Combining them requires large amounts of organic solvents or surfactants for solubilization, resulting in a sticky formula, poor skin feel, and insufficient long-term stability. For example, existing technologies improve solubility through cyclodextrin inclusion or alkaline salt formation, but the former requires complex processes and may reduce the concentration of active ingredients, while the latter easily damages the chemical stability of azelaic acid. Secondly, in formulation design, it is difficult to ensure that salicylic acid and azelaic acid coexist uniformly and stably in the same system, easily leading to precipitation, stratification, and other phenomena that affect product quality and efficacy. On the other hand, both salicylic acid (pH 3-4) and azelaic acid (pH 2-6) are acidic components. Using them together may damage the skin barrier, leading to symptoms such as dryness and dehydration. Long-term use can also thin the stratum corneum. Therefore, it is generally not recommended to use azelaic acid and salicylic acid together. Although some studies have attempted to add soothing ingredients such as panthenol, experiments have shown that they still cannot completely neutralize the irritation of the compound system, limiting the application of high concentrations.

[0004] These technological bottlenecks limit the development and application of products combining salicylic acid and azelaic acid. Innovative solutions are urgently needed to overcome these bottlenecks and meet consumer demand for highly effective whitening and acne-removing products. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the existing technology and provide a non-irritating acne-removing and whitening composition containing salicylic acid and azelaic acid.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows:

[0007] In a first aspect, the present invention provides an acne-removing and whitening composition comprising the following components in parts by weight: 4-8 parts azelaic acid, 0.1-4 parts salicylic acid, 0.1-4 parts dimethylmethoxybenzodihydropyranol, and 40-60 parts plant fermented oil; wherein the plant fermented oil is a mixture of macadamia nut (MACADAMIA TERNIFOLIA) seed oil and fermentation products of Candida albicans / glucose / rapeseed oleate.

[0008] Salicylic acid and azelaic acid are traditional acne-fighting ingredients. Azelaic acid focuses on anti-inflammation and pigmentation management, while salicylic acid is strong at unclogging pores. Both azelaic acid and salicylic acid work by regulating keratin metabolism and antibacterial properties. However, the technical threshold for combining these two ingredients is extremely high, especially at high concentrations, as they can easily irritate the skin. Dimethylmethoxybenzodihydropyranol has whitening effects and can fade acne scars by inhibiting endogenous human tyrosinase activity and providing photoprotection to the skin. This invention innovatively combines azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol into a plant fermented oil, successfully achieving a stable combination of salicylic acid and azelaic acid. This synergistically enhances the acne-fighting efficacy. The plant fermented oil added to the acne-removing and whitening composition of the present invention has a coating effect on salicylic acid, azelaic acid, and dimethylmethoxybenzodihydropyranol, forming reverse micelle microparticles with a microscopic diameter of less than 50nm (appearing transparent). This allows the above-mentioned active ingredients to work synergistically. The combination of azelaic acid and salicylic acid enhances the effect by regulating keratin metabolism and antibacterial activity. The three ingredients work in multiple directions, significantly improving the acne-removing effect, which is better than the effect of single-target action.

[0009] The skin's lipid layer is composed of keratinocytes, which are proteins with tightly packed lipids. Due to this structural feature, the skin layer both protects our body and serves as a crucial barrier for the penetration of active ingredients. This means that active ingredients should be in a similar state to the lipid layer to improve penetration. This invention utilizes plant fermented oils to encapsulate salicylic acid, azelaic acid, and dimethylmethoxybenzodihydropyranol, allowing them to easily penetrate the skin's lipid layer and reducing epidermal irritation.

[0010] Preferably, it further includes the following component in parts by weight: 24-55.8 parts of hexanediol.

[0011] Preferably, in the plant fermented oil preparation, the mass ratio of macadamia ternifera seed oil to fermentation product of Candida albicans / glucose / methyl rapeseed oil is 6:94.

[0012] More preferably, it comprises the following components in parts by weight: 5-8 parts azelaic acid, 2-4 parts salicylic acid, 2-4 parts dimethylmethoxybenzodihydropyranol, 50-60 parts plant fermentation oil, and 24-41 parts hexanediol.

[0013] Secondly, the present invention provides a method for preparing the acne-removing and whitening composition, comprising the following steps:

[0014] (1) Mix azelaic acid, salicylic acid, dimethylmethoxybenzodihydropyranol and hexanediol until they are dispersed and dissolved to obtain a mixture;

[0015] (2) Add plant fermented oil to the mixture obtained in step (1) and stir to obtain the acne-removing and whitening composition.

[0016] Preferably, step (1) involves heating to 80°C-95°C and stirring until completely dissolved.

[0017] Preferably, in step (2), the temperature is first lowered to 65℃-75℃ before adding the plant fermentation oil.

[0018] This invention first disperses and dissolves azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol in ethylene glycol, then adds them to a plant fermented oil and stirs. This process forms reverse micelles, allowing the plant fermented oil to encapsulate salicylic acid, azelaic acid, and dimethylmethoxybenzodihydropyranol. The transparent appearance confirms the formation of reverse micelles with a microscopic diameter of less than 50 nm, with no turbidity or precipitation, indicating complete encapsulation. This encapsulation helps the skin build tolerance to azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol, reducing the irritation of these three components to the epidermis and allowing them to better exert their acne-removing and whitening effects in the dermis. This invention utilizes reverse micelle encapsulation technology, which not only significantly improves the penetration efficiency of active ingredients but also effectively reduces irritation, opening a new path for acne product development and achieving "highly effective acne removal while being gentle on the skin." The acne-removing and whitening composition obtained by this invention provides rapid acne removal without causing skin damage, balancing efficacy and safety.

[0019] The plant-fermented oil of this invention enhances the polarity of the oil through fermentation, thereby obtaining a highly polar, oil-soluble reverse micelle encapsulation system. This increases the number of hydrophilic groups, enabling the encapsulation of various types of water-soluble / oil-soluble active ingredients and polymers, improving the stability of active ingredients, increasing transdermal absorption, and reducing irritation. The plant-fermented oil possesses an excellent natural oil-soluble sustained-release system. Upon contact with epidermal lipids, it exhibits lipophilic properties, maintaining a reverse micelle state, encapsulating components that are difficult to stabilize in oil, protecting them from dissolution in the skin's lipid layer, and delivering them to the dermis for further release. It also exhibits excellent water- and oil-soluble solubility: it can dissolve and encapsulate various types of water-soluble / oil-soluble active ingredients. The plant-fermented oil is a bio-fermentation product, natural, environmentally friendly, and green: through the fermentation metabolites of Candida albicans and natural plant oils, it dissolves and encapsulates active ingredients and polymers. Therefore, without the need for synthetic surfactants, various components can remain stable in the oil.

[0020] Preferably, the plant fermented oil of the present invention can be SynBiOil, which contains 94% of Candida albicans / glucose / rapeseed oleate fermentation product and 6% macadamia ternifera seed oil.

[0021] SynBiOil can encapsulate azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol, delivering these ingredients to the dermis layer of the skin before release, reducing irritation to the epidermis. SynBiOil has low viscosity and excellent spreadability, presenting a fresh and lightweight unique oil texture with strong moisturizing properties. Therefore, SynBiOil's reverse micelle encapsulation technology can bring a strong hydrating sensory experience to formulations that is not found in typical oil-based products.

[0022] Thirdly, the present invention provides the application of the above-mentioned acne-removing and whitening composition in acne-removing and whitening cosmetics.

[0023] Preferably, the dosage form of the cosmetic includes water, lotion, and cream.

[0024] Fourthly, the present invention provides an acne-removing and whitening cosmetic, comprising the above-mentioned acne-removing and whitening composition and excipients.

[0025] Preferably, the acne-removing and whitening composition accounts for 0.5%-10% of the total mass of the acne-removing and whitening cosmetic.

[0026] Concentration control: Azelaic acid not exceeding 5%, salicylic acid not exceeding 2%, dimethylmethoxybenzodihydropyranol not exceeding 2%. Sensitive skin needs to reduce the frequency (2-3 times per week).

[0027] The beneficial effects of this invention are as follows:

[0028] This invention innovatively incorporates azelaic acid, salicylic acid, and dimethylmethoxybenzodihydropyranol into a plant-fermented oil. The plant-fermented oil possesses reverse micellar encapsulation properties, which, even with a high ratio of these three active ingredients, reduces epidermal irritation and rapidly improves skin tolerance. This allows them to better exert their effects on acne treatment, fading acne scars, and whitening / removing blemishes in the dermis. The plant-fermented oil in this acne-removing and whitening composition encapsulates salicylic acid, azelaic acid, and dimethylmethoxybenzodihydropyranol, enabling synergistic effects after their combination. The combination of azelaic acid and salicylic acid enhances keratin metabolism and antibacterial efficacy. The multi-faceted action of these three ingredients significantly improves acne treatment, resulting in better effects than single-target treatments.

[0029] The acne-removing and whitening combination provided by this invention is used to prepare cosmetics. It has a refreshing and non-sticky feel, overturning the traditional oily feeling of acid-based products. It is suitable for sensitive skin and oily acne-prone skin, and can accurately and gently solve skin problems such as acne and roughness. Attached Figure Description

[0030] Figure 1 These are the results observed using transmission electron microscopy.

[0031] Figure 2 The results of the chicken embryo chorioallantoic membrane test are shown in the figures for negative and positive controls.

[0032] Figure 3 The results of the chicken embryo chorioallantoic membrane test in Example 2 and Comparative Example 4 are shown in the figure.

[0033] Figure 4 The image shows the results of the Propionibacterium acnes inhibition zone in the test groups of Examples 1-3.

[0034] Figure 5 The image shows the results of the inhibition zone of Propionibacterium acnes in the test groups 1-5. Detailed Implementation

[0035] To better illustrate the purpose, technical solution, and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0036] Unless otherwise specified, the experimental methods used in this invention are all conventional methods, and the materials and reagents used are all commercially available unless otherwise specified.

[0037] The raw materials used in this invention include:

[0038] Candida bombicola / glucose / methyl rapeseed oil fermentation product: INCI name is CANDIDABOMBICOLA / GLUCOSE / METHYL RAPESEEDATE FERMENT. This ingredient is listed in the "Catalogue of Used Cosmetic Ingredients (2021 Edition)" No. 03364.

[0039] Macadamia ternifolia seed oil: INCI name is Macadamia ternifolia esterified oil. This ingredient is listed in the "Catalogue of Used Cosmetic Ingredients (2021 Edition)" under serial number 01070.

[0040] The plant fermentation oil agent selected in this invention is SynBiOil (purchased from Baiyao Biotechnology), which contains 94% fermentation product of Candida albicans / glucose / rapeseed oleate and 6% macadamia ternifera seed oil.

[0041] The appearance and physicochemical properties of the acne-removing and whitening composition prepared in this invention are as follows:

[0042] Appearance: Light yellow to reddish transparent liquid;

[0043] Heat resistance (45±2℃): After 3 months, the temperature recovered and no abnormalities were observed.

[0044] Cold resistance (-15±2℃): After 3 months, the temperature recovered and no abnormalities were observed.

[0045] Cyclic test (45℃, room temperature, -5℃): 3 months, returned to room temperature, no abnormalities.

[0046] Observation using a transmission electron microscope (model: JEM-F200, manufacturer: JEOL, USA) showed that the acne-removing and whitening composition of the present invention can form reverse micelles with a microscopic diameter of less than 50 nm. Figure 1 ).

[0047] Examples 1-3:

[0048] Examples of the acne-removing and whitening composition of the present invention; the components and their mass ratios of the acne-removing and whitening compositions of Examples 1-3 are shown in Table 1.

[0049] Table 1 (Unit: mass percentage)

[0050] Ingredients Example 1 Example 2 Example 3 Azelaic acid 4 5 8 Salicylic acid 0.1 2 4 Dimethylmethoxychromanol 0.1 2 4 Plant ferment oil 40 50 60 Hexylene glycol To 100 To 100 To 100

[0051] The plant fermentation oil agent selected in this invention is SynBiOil, which contains 94% fermentation product of Candida albicans / glucose / rapeseed oleate and 6% macadamia ternifera seed oil.

[0052] The preparation method of the acne-removing and whitening composition includes the following steps:

[0053] (1) Mix azelaic acid, salicylic acid, dimethylmethoxybenzodihydropyranol and hexanediol, heat to 80℃-95℃, and stir until dispersed and dissolved to obtain a mixture;

[0054] (2) Add plant fermented oil to the mixture obtained in step (1) (first cool it to 65℃-75℃, or you can leave it cool) and stir to obtain the acne-removing and whitening composition.

[0055] Comparative Examples 1-5:

[0056] Comparative examples of the acne-removing and whitening compositions of the present invention; the components and their mass ratios of the acne-removing and whitening compositions of Comparative Examples 1-5 are shown in Table 2.

[0057] Table 2 (Unit: mass percentage)

[0058]

[0059] The only differences between the acne-removing and whitening compositions described in Comparative Examples 1-4 and Example 2 are: Comparative Example 1 does not contain azelaic acid; Comparative Example 2 does not contain salicylic acid; Comparative Example 3 does not contain dimethylmethoxybenzodihydropyranol; Comparative Example 4 does not contain plant fermented oil; and any missing components are made up with an equal amount of water.

[0060] The preparation method of the acne-removing and whitening composition is the same as in Example 2.

[0061] Test Example 1: Eye Irritation / Corrosiveness of Chicken Embryo Cholanosome Test

[0062] Test samples: Acne-removing and whitening compositions prepared in Example 2 and Comparative Example 4; negative control 0.9% NaCl sodium chloride; positive control 0.1 mol / L sodium hydroxide.

[0063] The test method refers to "SN / T 2329-2009 Cosmetic Eye Irritation / Corrosiveness Chicken Embryo Villi Allantoic Membrane Test".

[0064] Chicken embryo chorioallantoic membrane test (HET-CAM test) method:

[0065] 1. Basic Principles:

[0066] This experiment utilizes the intact, clear, and transparent chorioallantoic membrane vascular system of mid-stage hatched chicken embryos. A certain amount of the test substance is directly exposed to the chicken embryo, and after a period of time, changes in chorioallantoic membrane irritation indicators (such as hemorrhage, coagulation, and vascular dissolution) are observed. These indicators reflect changes in the morphology, structure, color, and permeability of blood vessels and vascular networks, as well as phenomena such as chorioallantoic membrane protein denaturation and the degree of damage. These indicators are then combined to obtain a score to assess the eye irritation of the test substance.

[0067] 2. CAM preparation:

[0068] Nine-day-old chicken embryos were candled. The location of the air cell was marked on the eggshell surface. The marked portion of the eggshell was peeled off using dental serrated forceps to expose the white egg membrane. A few mL of 0.9% sodium chloride (NaCl) solution was added with a pipette to moisten the egg membrane, and then the 0.9% sodium chloride solution was poured out. The inner membrane was carefully removed with forceps, and the structure of the vascular system was observed again, and its integrity and suitability for testing were assessed.

[0069] 3. Formal Trial (Endpoint Evaluation Method)

[0070] Apply 0.3 mL of the test substance directly to the CAM, ensuring that at least 50% of the CAM surface is covered by the test substance. After 3 minutes of application, gently rinse the CAM membrane with physiological saline and observe the degree of change in each irritant effect. If the total score of 6 chicken embryos is moderate or above (total score ≥12), the test should be repeated once.

[0071] 4. Results observation and scoring criteria are shown in Table 3:

[0072] Table 3 Scoring Criteria

[0073]

[0074]

[0075] 5. For experiments using the endpoint evaluation method, the endpoint score (ES) should be calculated: the score per chicken embryo = the sum of the observed hemorrhage, coagulation and vascularization in each chicken embryo;

[0076] ES = the mathematical sum of the scores of the 6 chicken embryos.

[0077] The eye irritation of the test substances was classified according to the ES value (Table 4).

[0078] Table 4. ES Value Evaluation Criteria (HET-CAM)

[0079] End score ES value Irritation classification ES < 12 No / mild irritation 12 < ES < 16 Moderate irritation

[0080] 6. Test results are as followsFigure 2-3 As shown in Table 5.

[0081] Table 5

[0082]

[0083]

[0084] The results showed that the response results of the negative and positive controls were within the non-irritating and irritating ranges, and the selected chicken embryos met the testing requirements. The endpoint score (ES = Endpoint score) of the test sample in Example 2 was 0.00, indicating no irritation; the endpoint score (ES = Endpoint score) of the test sample in Comparative Example 4 was 10.00, indicating mild irritation.

[0085] Test Example 2: Whitening and Spot-Removing Efficacy Test

[0086] 1. Experimental Objective and Principle

[0087] The method for evaluating tyrosinase inhibitory activity is an in vitro simulation method for determining skin melanin content. In the biosynthesis of skin melanin, tyrosinase acts on dopa to form dopaquinone, which is ultimately formed into melanin. Tyrosinase catalyzes the conversion of dopa to dopaquinone in a phosphoric acid solution at pH 6.8. The absorbance can be measured at 475 nm using a spectrophotometer. The degree of change in absorbance is linearly related to the tyrosinase inhibitory activity; that is, the stronger the tyrosinase inhibitory activity, the lower the absorbance.

[0088] 2. Test Indicators

[0089] If the inhibition rate of tyrosinase in the test sample is greater than that in the negative control, and the statistical analysis shows P < 0.05, then a significant difference is considered.

[0090] 3. Test methods

[0091] (1) Reagent preparation

[0092] The test samples were the acne-removing and whitening compositions prepared in Examples 1-3 and Comparative Examples 1-5.

[0093] Instruments and reagents: analytical balance, 10mL test tubes; adjustable pipette, constant temperature water bath, UV-Vis spectrophotometer, pH 6.8 disodium hydrogen phosphate-citric acid buffer, tyrosinase, levodopa, positive control: kojic acid.

[0094] The positive control was diluted with pH 6.8 disodium hydrogen phosphate-citrate buffer to prepare a series of concentration gradients of 1 mg / mL, 0.2 mg / mL, 0.04 mg / mL, and 0.008 mg / mL to validate the test system.

[0095] Test substance treatment: The test substance was treated with the stock solution (disodium hydrogen phosphate-citric acid dilution as a negative control).

[0096] (2) Referring to Table 6, set up sample tubes (T), sample background (T0), enzyme reaction tubes (C), and solvent background (C0) using 10mL test tubes. For each sample, three parallel tubes should be set up for each test concentration of the sample tube (T), and three parallel tubes should also be set up for the enzyme reaction tube (C). Add 1mL of sample solution of the same concentration to each of the sample tubes (T) and sample background (T0), and add 1mL of disodium hydrogen phosphate-citrate buffer to each of the enzyme reaction tubes (C) and solvent background (C0).

[0097] Add 0.5 mL of tyrosinase solution to each of the sample tube (T) and enzyme reaction tube (C). Replace the sample background (T0) and solvent background (C0) with 0.5 mL of disodium hydrogen phosphate-citric acid buffer. Mix the sample and tyrosinase thoroughly and incubate in a 37°C water bath for 10 minutes.

[0098] Add 2 mL of levodopa solution to each tube in sequence, control the reaction time of each tube to 5 minutes, and immediately transfer the reaction solution of each tube into a cuvette and measure the absorbance at 475 nm.

[0099] Table 6 Sample Addition Requirements

[0100]

[0101] 4. Calculate the tyrosinase inhibition rate according to formula (1):

[0102]

[0103] In the above formula:

[0104] T - Sample background absorbance, which is the absorbance of the solution after the sample reacts with tyrosinase;

[0105] T0 - Sample background absorbance;

[0106] The absorbance of the C-enzyme reaction tube is the average of three measurements, i.e., the absorbance of the tyrosinase and dopa reactions without the addition of sample; the test result is the arithmetic mean of the three data points, and the SD value of the three data points must be ≤3%.

[0107] The results are shown in Table 7.

[0108] Table 7

[0109]

[0110] The results showed that the tyrosinase inhibition rate of the acne-removing and whitening compositions prepared in the embodiments of the present invention was greater than 45.27%, which was significantly different from the negative control (P < 0.05), indicating that the acne-removing and whitening compositions prepared in the embodiments of the present invention have significant whitening and spot-removing effects. Azelaic acid has anti-inflammatory effects, salicylic acid is effective in unclogging pores, and dimethylmethoxybenzodihydropyranol lightens acne marks, blemishes, and common pigmentation by inhibiting endogenous human tyrosinase activity and providing photoprotection to the skin. The plant fermented oil added to the acne-removing and whitening compositions of the present invention has a coating effect on salicylic acid, azelaic acid, and dimethylmethoxybenzodihydropyranol, so that the above-mentioned active ingredients can work synergistically after combination. The combination of azelaic acid and salicylic acid enhances the effect by regulating keratin metabolism and antibacterial activity; the three ingredients work in multiple ways, significantly improving the acne-removing effect, which is better than the effect of single-target action. Compared with the samples of Comparative Examples 1-5, the tyrosinase inhibition rate of Comparative Examples 1-5 was significantly reduced in Example 2, indicating that the proportions of azelaic acid, salicylic acid, dimethylmethoxybenzodihydropyranol, and plant fermented oil were outside the scope of this invention or lacked any of the components, thus failing to achieve the whitening and freckle-removing effects of this invention.

[0111] Test Example 3: Evaluation of Acne Treatment Efficacy

[0112] 1. Experimental Objective and Principle

[0113] Based on the fact that *Propionibacterium acnes* is a normal component of the hair follicle flora, and that *Propionibacterium acnes* proliferates during the inflammatory process of acne, this study tested the antibacterial effect of *Propionibacterium acnes* in samples to evaluate their acne-reducing efficacy. *Propionibacterium acnes*, a component of the normal hair follicle flora, uses sebum as its active ingredient, partially converting it into free fatty acids. *Propionibacterium acnes* can also activate the complement pathway, producing at least two chemokines that promote inflammation. Therefore, inhibiting *Propionibacterium acnes* can achieve an acne-reducing effect. Different concentration gradients were created by continuously dissolving the antibacterial agent and diffusing it through agar to demonstrate its antibacterial effect. The size of the inhibition zone was used to determine whether the agent possessed antibacterial ability.

[0114] 2. Test Indicators

[0115] The test samples were the acne-removing and whitening compositions prepared in Examples 1-3 and Comparative Examples 1-5.

[0116] The antibacterial ability of Propionibacterium acnes was evaluated by the size of the inhibition ring in the experimental group. An inhibition ring diameter greater than 7 mm was judged to have an antibacterial effect; an inhibition ring diameter less than or equal to 7 mm was judged to have no antibacterial effect.

[0117] 3. Test strains

[0118] The third-generation Propionibacterium acnes (ATCC11827) was provided by Guangdong Huankai Technology Co., Ltd.

[0119] 4. QB / T 2738-2023 "Evaluation Method for Antibacterial and Bacteriostatic Effects of Daily Chemical Products" 7.5 Inhibition Ring Test.

[0120] method:

[0121] (1) Preparation of test group samples: Add 20 μL of the actual concentration of the sample to each sample, then place the filter paper flat in a clean sterile petri dish, open the lid and bake it in an incubator (37℃) to dry, or let it dry naturally at room temperature for later use. Each group consists of 4 samples.

[0122] (2) Preparation of negative control samples: Take sterile dry filter paper, add 20 μL of sterile distilled water to each paper, and dry it for later use.

[0123] (3) Inoculation of test bacteria: Use a sterile cotton swab to collect a concentration of 5×10⁻⁶ bacteria. 5 ~5×10 6 The test bacterial suspension was evenly spread three times on the surface of a Clostridium perfringens agar plate. After each spread, the plate was rotated 60°. Finally, a cotton swab was used to swab around the edge of the plate. The plate was then covered and allowed to dry at room temperature for 5 minutes.

[0124] (4) Sample Placement: Place 4 test samples and 1 negative control sample on each plate, for a total of 5 samples. Use sterile forceps to place the samples onto the plate surface. The center of each sample should be at least 25 mm apart, and the sample should be at least 15 mm away from the perimeter of the plate. After placement, gently press the samples with sterile forceps to ensure they adhere tightly to the plate surface. Cover the petri dish and incubate at 37℃ for 48 hours in anaerobically to observe the results.

[0125] (5) Measure and record the diameter of the inhibition zone (including the patch) using vernier calipers. Repeat the test three times. When measuring the inhibition zone, select a uniform and completely sterile inhibition zone. The diameter should be measured from the outer edge of the inhibition zone.

[0126] Table 8

[0127]

[0128]

[0129] The results are shown in Table 8. Figure 4-5As shown. The results indicate that the average diameter of the inhibition zone of the test samples of the acne-removing and whitening composition prepared in the embodiments of the present invention is greater than 10.44 mm, which is significantly different from the negative control. This indicates that the acne-removing and whitening composition prepared in the embodiments of the present invention has an inhibitory effect on Propionibacterium acnes and has a significant acne-removing effect. Compared with the samples of Comparative Examples 1-5, the average diameter of the inhibition zone of Comparative Examples 1-5 is significantly reduced, indicating that the proportions of azelaic acid, salicylic acid, dimethylmethoxybenzodihydropyranol, and plant fermented oil are outside the scope of the present invention or that any component is missing, thus failing to achieve the acne-removing effect of the present invention.

[0130] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A composition for acne and whitening, characterized by comprising, The composition comprises the following components by weight: azelaic acid 4-8 parts, salicylic acid 0.1-4 parts, dimethyl methoxy chromanol 0.1-4 parts, plant ferment oil 40-60 parts, hexanediol 24-55.8 parts; the plant ferment oil is a mixture of MACADAMIA TERNIFOLIA seed oil and Candida bombicola / glucose / rapeseed oil acid methyl ester fermentation product.

2. The anti-acne whitening composition according to claim 1, wherein In the plant ferment oil, the mass ratio of MACADAMIA TERNIFOLIA seed oil to Candida bombicola / glucose / rapeseed oil acid methyl ester fermentation product is MACADAMIA TERNIFOLIA seed oil: Candida bombicola / glucose / rapeseed oil acid methyl ester fermentation product = 6:

94.

3. The anti-acne whitening composition according to claim 1, wherein the composition comprises 0.01 to 5% by weight of the at least one vitamin C derivative. The composition comprises the following components by weight: azelaic acid 5-8 parts, salicylic acid 2-4 parts, dimethyl methoxy chromanol 2-4 parts, plant ferment oil 50-60 parts, hexanediol 24-41 parts.

4. A process for the preparation of the anti-acne whitening composition according to any one of claims 1 to 3, characterized in that, The method comprises the following steps: (1) mixing azelaic acid, salicylic acid, dimethyl methoxy chromanol and hexanediol to obtain a mixture; (2) adding plant ferment oil to the mixture obtained in step (1) to obtain the acne-removing and whitening composition.

5. Use of the acne-removing and whitening composition according to any one of claims 1-3 in the preparation of an acne-removing and whitening cosmetic.

6. The use according to claim 5, wherein the compound is ###0002### The dosage form of the cosmetic comprises water, milk and cream.

7. An anti-acne whitening cosmetic, characterized by, The acne-removing and whitening cosmetic comprises the acne-removing and whitening composition according to any one of claims 1-3 and adjuvants.

8. The anti-acne whitening cosmetic according to claim 7, wherein The mass of the acne-removing and whitening composition accounts for 0.5%-10% of the total mass of the acne-removing and whitening cosmetic.

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