Method for preparing anti-diarrheal disease feed from beer brewing waste and application of anti-diarrheal disease feed

Abstract

The invention provides a method for preparing an anti-diarrheal disease feed from beer brewing waste and application, and belongs to the technical field of microbial fermentation feeds, and particularly relates to a method for preparing an anti-diarrheal disease feed by mixing 3-4 parts of brewer's grains, 3-4 parts of corn kernels, 2-4 parts of soybean meal, 1-2 parts of beer waste yeast powder, 1-2 parts of rice bran and 1-2 parts of wheat bran as a fermentation raw material. Adding a waste beer yeast washing solution-cyclodextrin solution, a waste beer yeast agent, bacillus subtilis and lactobacillus acidophilus, and carrying out anaerobic solid fermentation to prepare the anti-diarrhea feed. The prepared feed can significantly increase the weight gain of the fattening pigs and reduce the feed-gain ratio and the diarrhea rate.

Description

Technical Field

[0001] The present invention belongs to the technical field of microbial fermentation feed, and particularly relates to a method for preparing an anti-diarrhea feed by using beer brewing waste and its application. Background Art

[0002] A large amount of beer grains and waste yeast are generated during the beer brewing process, accounting for about 90% of the total waste. On average, 110 - 130 kg of beer grains can be obtained from every 100 kg of malt fed for brewing, and its water content is about 80%. The main components of beer grains are malt husks and un-glycated malt, as well as high-molecular substances that are not easily soluble in the auxiliary materials. Among them, the protein content is about 25%, the cellulose content is above 17%, and there are also various nutrients such as vitamins and trace elements. Beer waste yeast also contains 50% protein, 16% cellulose, 8.2% fat, 6 - 8% nucleic acid, 5% minerals, 2% vitamins, as well as glutathione and coenzyme A, etc. The beer grains just produced by the brewery must be processed as soon as possible. Otherwise, due to their high water content and rich nutrients, they are easily contaminated by microorganisms and rot, causing environmental pollution, and the nutritional value will also be reduced. Generally, breweries sell the wet beer grains immediately after production directly as fertilizers or feeds. If they cannot be sold and used in time, it is easy to cause waste of resources and environmental pollution. A small number of manufacturers dehydrate the beer grains before selling them, but the energy consumption is large and the cost is high. Currently, a relatively effective method for utilizing these beer brewing wastes is to re-ferment the beer grains to produce feeds or fertilizers, increase the protein and nutrient content of the feeds, achieve the maximum recycling of waste, and at the same time reduce environmental pollution.

[0003] Beer waste yeast refers to the yeast that can no longer be used as a beer fermentation strain after the beer fermentation ends. These yeasts settle and are discharged from the fermentation tank, and bio-functional factors such as humulone precipitated during the fermentation process adhere to their surfaces. Beer waste yeast contains a variety of nutrients such as rich amino acids, carbohydrates, fats, crude fiber, and minerals. It is often made into dry yeast, or yeast extract is prepared after autolysis, and is widely used in industries such as food, medicine, and feed. It can also be directly fed as a feed, or made into edible nutritional products.

[0004] There are many types of bioactive substances in hops. During the wort boiling process, they interact with the components in the wort and undergo complex chemical changes. Some of them precipitate as heat coagulants and are discharged. During the subsequent fermentation process, these substances further change. As the pH of the beer decreases during the fermentation process, some other components precipitate and are discharged together with the waste yeast, including α-acids and dozens of other active components. These substances are usually discharged due to the difficulty of recycling, resulting in great waste.

[0005] The invention patent "A Method for Producing Enzyme-Rich Protein Feed from Brewer's Grains" uses single brewer's grains as raw materials, adds waste brewer's yeast and Aspergillus niger for fermentation to prepare enzyme-rich protein feed. The raw materials are single and do not have disease-resistant effects. Moreover, directly adding waste brewer's yeast, the bitter substances such as α-acids on its surface affect the taste of the feed and are not conducive to promoting animal appetite; some other bioactive substances lose their activity during feed storage. Summary of the Invention

[0006] In view of the deficiencies of the prior art, the present invention provides a method for preparing anti-diarrhea feed using beer brewing waste and its application.

[0007] The technical solution of the present invention is as follows:

[0008] A method for preparing anti-diarrhea feed using beer brewing waste, comprising the following steps:

[0009] (1) Rinse waste brewer's yeast with water, take the supernatant after centrifugation, and add cyclodextrin to the supernatant to obtain a waste brewer's yeast washing solution-cyclodextrin solution; take a part of the washed waste brewer's yeast as a microbial agent for standby; dry the remaining washed waste brewer's yeast to become waste brewer's yeast powder;

[0010] (2) Weigh 3-4 parts of brewer's grains, 3-4 parts of corn kernels, 2-4 parts of soybean meal, 1-2 parts of waste brewer's yeast powder, 1-2 parts of rice bran and 1-2 parts of wheat bran by weight, crush and mix them to obtain fermentation raw materials;

[0011] (3) Inoculate the washed waste brewer's yeast microbial agent prepared in step (1), Bacillus subtilis and Lactobacillus acidophilus into the fermentation raw materials prepared in step (2); then add the waste brewer's yeast washing solution-cyclodextrin solution prepared in step (1), mix well, and perform anaerobic solid fermentation;

[0012] (4) After fermentation is completed, dry it to obtain anti-diarrhea feed.

[0013] Preferably according to the present invention, in step (1), the waste brewer's yeast is rinsed with 1-10 times the weight of sterile water or physiological saline to obtain a rinsing solution and washed waste brewer's yeast; add cyclodextrin to the rinsing solution, and the addition concentration of cyclodextrin is 0.5%-5% of the rinsing solution by mass fraction, and stir at 200-280 rpm for 1-4 h; reserve the washed waste brewer's yeast according to the specific dosage as a microbial agent for standby, and dry the remaining washed waste brewer's yeast at 55-70 °C to become yeast dry powder.

[0014] More preferably, reserve the washed waste brewer's yeast according to the specific dosage and store it at 4 °C under sterile conditions as a microbial agent for standby.

[0015] Preferably according to the present invention, in step (1), the rinsing method is to mix sterile water or physiological saline with waste beer yeast evenly, then centrifuge at a speed above 800 rpm to obtain the supernatant as the rinsing solution, and the remaining is the washed waste beer yeast.

[0016] Preferably according to the present invention, in step (1), the cyclodextrin is β-cyclodextrin and / or hydroxypropyl-β-cyclodextrin.

[0017] Preferably according to the present invention, in step (2), after crushing and mixing, it is sieved through a 40-80 mesh sieve to obtain the fermentation raw material.

[0018] Preferably according to the present invention, in step (3), by weight, 10-30 parts of the washed waste beer yeast prepared in step (1), 1-3 parts of Bacillus subtilis, 1-3 parts of Lactobacillus acidophilus, 100-150 parts of the fermentation raw material, and 100-250 parts of the waste beer yeast washing solution-cyclodextrin solution are mixed, and anaerobic solid fermentation is carried out at 30-35 °C for 96-120 h.

[0019] Preferably according to the present invention, the anaerobic solid fermentation is carried out in a sealed container with a breathing valve.

[0020] More preferably, the sealed container with a breathing valve is a sealed bag with a breathing valve.

[0021] Preferably according to the present invention, in step (4), the drying temperature is 30-78 °C.

[0022] The yeast used in the present invention is a commercially available ordinary beer yeast commonly used in breweries, and the Bacillus subtilis and Lactobacillus acidophilus use commercially available commercialized bacterial powders that can be used to prepare animal feed.

[0023] Application of the anti-diarrhea feed prepared by the above method in livestock breeding.

[0024] Preferably according to the present invention, the livestock is a pig.

[0025] More preferably, the pig is a fattening pig.

[0026] The beneficial effects of the present invention at least include the following:

[0027] 1. The present invention provides a method and application for preparing an anti-diarrhea feed using beer brewing waste. Using beer grains, etc. as the main fermentation raw materials and waste beer yeast as the main source of strains. Adding the hop and wort active ingredients that settle with the yeast, these ingredients have undergone complex changes during the wort boiling and fermentation processes. The inventor found that their effects on preventing livestock diarrhea, etc. are significantly better than adding hops, humulone, or other bioactive substances that settle during other stages of beer brewing alone. The feed prepared by the present invention can significantly increase the weight gain of fattening pigs, reduce the feed-to-weight ratio and diarrhea rate.

[0028] 2. The production method provided by the present invention is simple, with low cost, environmentally friendly and efficient. The product is rich in nutrients such as amino acids, cellulose, and minerals, and has broad market prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a graph showing the effect of the anti-diarrhea feed prepared from beer brewing waste on the total weight gain of fattening pigs;

[0030] In the figure: * indicates p < 0.05 compared with the control group; # indicates p < 0.05 compared with experimental group 1.

[0031] Figure 2 It is a graph showing the effect of the anti-diarrhea feed prepared from beer brewing waste on the feed conversion ratio of fattening pigs;

[0032] In the figure: * indicates p < 0.05 compared with the control group; # indicates p < 0.05 compared with experimental group 1.

[0033] Figure 3 It is a graph showing the effect of the anti-diarrhea feed prepared from beer brewing waste on the diarrhea rate of fattening pigs;

[0034] In the figure: * indicates p < 0.05 compared with the control group; # indicates p < 0.05 compared with experimental group 1. DETAILED DESCRIPTION OF THE INVENTION

[0035] The present invention will be further described in detail below with reference to specific embodiments, but the scope of protection required by the present invention is not limited thereto.

[0036] For the content without specific conditions in the embodiments, it is carried out according to conventional conditions; for the reagents or instruments without indicating the manufacturer, they are all ordinary commercially available products.

[0037] Source of Materials

[0038] The spent grains, beer waste yeast (the beer is fermented with Fermentis Ale dry yeast US-05, and after the beer cools down at the end of fermentation, the yeast settles to the bottom of the fermentation tank, and the waste yeast is taken from the bottom of the fermentation tank), and wort boiling hot coagulum are provided by Jinan Lanpu Brewing Technology Co., Ltd.; the spent grains used in Example 2 are prepared by saccharifying barley malt and wheat in a ratio of 1:1; the spent grains used in other examples and comparative examples are prepared by saccharifying barley malt.

[0039] The hops (Qingdao Dahua) are purchased from Jinan Lufeng Beer Raw Material Co., Ltd.

[0040] The soybean meal, rapeseed meal, fish meal, corn kernels, rice bran, wheat bran, etc. are purchased from Shandong Wuxiaozheng Ecological Agriculture Technology Co., Ltd.

[0041] β-cyclodextrin and hydroxypropyl-β-cyclodextrin were purchased from Shandong Binzhou Zhiyuan Biotechnology Co., Ltd.

[0042] Bacillus subtilis (20 billion / g) and Lactobacillus acidophilus (5 billion / g) were purchased from the Minquan Branch of Xuzhou Saifu Biotechnology Co., Ltd.

[0043] The fermentation feed bag with a breathing valve was purchased from Jiangmen Henghou Plastic Products Co., Ltd.

[0044] The diet was an ordinary commercially available feed product.

[0045] Example 1

[0046] (1) Treatment of beer waste yeast: Take beer waste yeast (with a water content of about 90%), rinse the waste yeast with 2 times the weight of sterile physiological saline, take the supernatant after centrifugation at 1000 rpm for 2 min, add 1% β-cyclodextrin by mass fraction to the supernatant, and then stir at 200 rpm for 2 h to obtain a beer waste yeast washing-β-cyclodextrin solution; Reserve the washed beer waste yeast (with a water content of about 90%) under sterile conditions at 4°C for use as a microbial agent according to the specific dosage; The remaining washed beer waste yeast was dried at 65°C to become beer waste yeast dry powder.

[0047] (2) Preparation of fermentation raw materials: By weight, 3 parts of beer grains, 3 parts of corn kernels, 3 parts of soybean meal, 1 part of beer waste yeast dry powder, 1 part of rice bran and 1 part of wheat bran were fully pulverized and stirred evenly, and passed through a 40-mesh sieve to obtain fermentation raw materials.

[0048] (3) Anaerobic solid fermentation culture: Inoculate the washed beer waste yeast microbial agent prepared in step (1), Bacillus subtilis and Lactobacillus acidophilus into the fermentation raw materials prepared in step (2). Specifically, by weight, 10 parts of the washed beer waste yeast microbial agent prepared in step (1) (with a water content of about 90%), 1 part of Bacillus subtilis, 1 part of Lactobacillus acidophilus and 100 parts of fermentation raw materials and 140 parts of the beer waste yeast washing-β-cyclodextrin solution prepared in step (1) were stirred and mixed evenly, then filled into a fermentation feed bag with a breathing valve, and solid-fermented at 30°C for 96 h.

[0049] (4) Drying of fermentation products: After the fermentation was completed, it was dried at 60°C to obtain an anti-diarrhea feed prepared from beer brewing waste.

[0050] Example 2

[0051] (1) Treatment of beer waste yeast: Take beer waste yeast (with a water content of about 90%), rinse the beer waste yeast with 3 times its weight of sterile physiological saline, take the supernatant after centrifuging at 1200 rpm for 1 min, add β-cyclodextrin with a mass fraction of 1.5% to the supernatant, and then stir at 260 rpm for 3 h to obtain a beer waste yeast washing solution-β-cyclodextrin solution; Reserve the washed beer waste yeast (with a water content of about 90%) according to the specific dosage and store it at 4°C under sterile conditions for use as a microbial agent, and dry the remaining washed beer waste yeast at 70°C to become yeast dry powder.

[0052] (2) Preparation of fermentation raw materials: Weigh 4 parts of beer grains, 4 parts of corn kernels, 2 parts of soybean meal, 2 parts of beer waste yeast dry powder, 2 parts of rice bran and 2 parts of wheat bran, crush and stir them evenly, and pass through an 80-mesh sieve to obtain fermentation raw materials.

[0053] (3) Anaerobic solid fermentation culture: Inoculate the washed beer waste yeast microbial agent prepared in step (1), Bacillus subtilis, and Lactobacillus acidophilus into the fermentation raw materials prepared in step (2). Specifically, by weight, take 30 parts of the washed beer waste yeast microbial agent prepared in step (1) (with a water content of about 90%), 1.5 parts of Bacillus subtilis, 1.5 parts of Lactobacillus acidophilus, 150 parts of fermentation raw materials, and then add 200 parts of the beer waste yeast washing solution-β-cyclodextrin solution prepared in step (1). After stirring and mixing evenly, put it into a fermentation feed bag with a breathing valve and carry out solid fermentation at 35°C for 120 h.

[0054] (4) Drying of fermentation products: After the fermentation is completed, dry at 75°C to obtain an anti-diarrhea feed prepared from beer brewing waste.

[0055] Example 3

[0056] (1) Treatment of beer waste yeast: Take beer waste yeast (with a water content of about 90%), rinse the beer waste yeast with 5 times its weight of sterile water, take the supernatant after centrifuging at 800 rpm for 3 min, add hydroxypropyl-β-cyclodextrin with a mass fraction of 2.5% to the supernatant, and then stir at 280 rpm for 1 h to obtain a beer waste yeast washing solution-hydroxypropyl-β-cyclodextrin solution; Reserve the washed beer waste yeast (with a water content of about 90%) according to the specific dosage and store it at 4°C under sterile conditions for use as a microbial agent, and dry the remaining washed beer waste yeast at 60°C to become beer waste yeast dry powder.

[0057] (2) Preparation of fermentation raw materials: Weigh 3.5 parts of beer grains, 3.5 parts of corn kernels, 4 parts of soybean meal, 1 part of beer waste yeast dry powder, 2 parts of rice bran and 2 parts of wheat bran, crush and stir them evenly, and pass through a 60-mesh sieve to obtain fermentation raw materials.

[0058] (3) Anaerobic solid fermentation culture: Inoculate the washed waste beer yeast agent prepared in step (1), Bacillus subtilis, and Lactobacillus acidophilus into the fermentation raw materials prepared in step (2). Specifically, by weight, add 20 parts (with a water content of about 90%) of the washed waste beer yeast agent prepared in step (1), 1 part of Bacillus subtilis, 2 parts of Lactobacillus acidophilus, 140 parts of fermentation raw materials, and then add 250 parts of the waste beer yeast washing solution - hydroxypropyl-β-cyclodextrin solution prepared in step (1). After stirring and mixing evenly, pack it into a fermentation feed bag with a breathing valve and conduct solid fermentation at 32°C for 96 h.

[0059] (4) Drying of fermentation products: After fermentation, dry at 50°C to obtain the anti-diarrhea feed prepared from beer brewing waste.

[0060] Comparative Example 1

[0061] In step (2), do not add beer grains and waste beer yeast dry powder, and replace them with 3 parts of rapeseed meal and 1 part of fish meal, and the others are the same as in Example 1.

[0062] Comparative Example 2

[0063] In step (3), do not add the yeast washing solution - β-cyclodextrin solution, and replace it with 140 parts of a 1% (mass fraction) β-cyclodextrin solution, and the others are the same as in Example 1.

[0064] Comparative Example 3

[0065] In step (3), do not add the yeast washing solution - β-cyclodextrin solution, and replace it with 140 parts of a β-cyclodextrin solution containing 10 mg / mL of α-acid, and the others are the same as in Example 1.

[0066] Preparation method of the β-cyclodextrin solution containing 10 mg / mL of α-acid: Add α-acid to sterile physiological saline to obtain an α-acid solution with a final concentration of 10 mg / mL, and then add 1% (mass fraction) of β-cyclodextrin and stir at 200 rpm for 2 h to obtain the β-cyclodextrin solution containing 10 mg / mL of α-acid.

[0067] Comparative Example 4

[0068] In step (3), do not add the yeast washing solution - β-cyclodextrin solution, and replace it with 140 parts of a β-cyclodextrin solution of wort boiling hot coagulum washing solution, and the others are the same as in Example 1;

[0069] Preparation method of wort boiling hot coagulum washing liquid-β-cyclodextrin solution: Take the wort boiling hot coagulum precipitated in the whirlpool settling tank, rinse the hot coagulum with 2 times the weight of sterile physiological saline, take the supernatant after centrifuging at 1000 rpm for 2 min, add β-cyclodextrin with a mass fraction of 1%, and then stir at 200 rpm for 2 h to obtain the hot coagulum washing liquid-β-cyclodextrin solution, which is stored at 4°C for standby.

[0070] Comparative Example 5

[0071] In step (3), no yeast washing liquid-β-cyclodextrin solution is added, and it is replaced with 140 parts of hop boiling water-β-cyclodextrin solution, and the others are the same as in Example 1;

[0072] Preparation method of hop boiling water-β-cyclodextrin solution: The following steps are included: Take 10 g of hops, add 500 mL of water, boil for 30 min, take the supernatant after centrifuging at 1000 rpm for 2 min and add β-cyclodextrin with a mass fraction of 1%, and then stir at 200 rpm for 2 h to obtain the hop water-β-cyclodextrin solution, which is stored at 4°C for standby.

[0073] Comparative Example 6

[0074] In step (1), β-cyclodextrin is not added, and in step (4), 140 parts of the yeast washing liquid prepared in step (1) are directly added, and the others are the same as in Example 1.

[0075] Comparative Example 7

[0076] In step (1), β-cyclodextrin is not added, and it is replaced with maltodextrin with a mass fraction of 1% to obtain the yeast washing liquid-maltodextrin solution.

[0077] In step (3), 140 parts of the yeast washing liquid-maltodextrin solution prepared in step (1) are directly added, and the others are the same as in Example 1.

[0078] Comparative Example 8

[0079] (1) Preparation of fermentation raw materials: Weigh 4 parts of rice bran, 3 parts of corn kernels, and 3 parts of soybean meal by weight, fully pulverize and stir them evenly, and pass through a 40-mesh sieve to obtain the fermentation raw materials.

[0080] (2) Anaerobic solid fermentation culture: Inoculate the unrinsed beer waste yeast, Bacillus subtilis, and Lactobacillus acidophilus into the fermentation raw materials prepared in step (1). Specifically, by weight, 10 parts of the unrinsed beer waste yeast agent (with a water content of about 90%), 2 parts of Bacillus subtilis, 1 part of Lactobacillus acidophilus, 100 parts of the fermentation raw materials, and then add 140 parts of sterile water. After stirring and mixing evenly, it is filled into a fermentation feed bag with a breathing valve and solid-fermented at 30°C for 96 h.

[0081] (3) Drying of fermentation product: After the fermentation is completed, it is dried at 60 °C to obtain an anti-diarrhea feed prepared from beer brewing waste.

[0082] Application example

[0083] Effect of adding the fermented feed prepared by the present invention on the growth status and diarrhea rate of fattening pigs. The test method is as follows:

[0084] Healthy male pigs of similar body weight at 2 months old were selected for the test and randomly divided into several groups, with 15 pigs in each group. They were fed normal feed or mixed feed (normal feed + a certain proportion of the feed prepared in the example or comparative example).

[0085] The experimental groups are as follows:

[0086] Control group: 100% diet.

[0087] Experimental group 1: 90% diet + 10% fermented feed prepared in Example 1;

[0088] Experimental group 2: 90% diet + 10% fermented feed prepared in Example 2;

[0089] Experimental group 3: 90% diet + 10% fermented feed prepared in Example 3;

[0090] Experimental group 4: 90% diet + 10% fermented feed prepared in Comparative Example 1;

[0091] Experimental group 5: 90% diet + 10% fermented feed prepared in Comparative Example 2;

[0092] Experimental group 6: 90% diet + 10% fermented feed prepared in Comparative Example 3;

[0093] Experimental group 7: 90% diet + 10% fermented feed prepared in Comparative Example 4;

[0094] Experimental group 8: 90% diet + 10% fermented feed prepared in Comparative Example 5;

[0095] Experimental group 9: 90% diet + 10% fermented feed prepared in Comparative Example 6;

[0096] Experimental group 10: 90% diet + 10% fermented feed prepared in Comparative Example 7;

[0097] Experimental group 11: 90% diet + 10% fermented feed prepared in Comparative Example 8;

[0098] The test period was 60 days. The pigs were weighed on an empty stomach at the beginning and end of the test to calculate the total weight gain and feed-to-weight ratio. The pigs were observed for diarrhea every day, the number of diarrhea pigs was recorded, and the diarrhea rate was calculated.

[0099] (1) Total weight gain (kg / head) = Final weight of pigs - Initial weight of pigs;

[0100] (2) Feed conversion ratio = Total feed intake within 60 days / Total weight gain within 60 days;

[0101] (3) Diarrhea rate (%) = Number of pigs with diarrhea / (Total number of pigs × Experimental days) × 100%;

[0102] The results are shown in Figure 1 、 Figure 2 、 Figure 3 。

[0103] Through Figure 1 and Figure 2 results, it can be seen that compared with the control group, in the groups fed with the fermented feed prepared in Examples 1 - 3, the total weight gain of the fattening pigs increased and the feed conversion ratio decreased; compared with the experimental groups fed with the feed prepared in Example 1, in the experimental groups fed with the feed prepared in Comparative Examples 1 - 8, the total weight gain of the fattening pigs decreased and the feed conversion ratio increased.

[0104] From Figure 3 it can be seen that compared with the control group, in the fattening pigs fed with the fermented feed prepared in Examples 1 - 3, the diarrhea rate was lower, indicating that their anti - diarrhea ability was improved; compared with the experimental groups fed with the feed prepared in Example 1, in the experimental groups fed with the feed prepared in Comparative Examples 2 - 8, the diarrhea rate increased, indicating that the anti - diarrhea effect decreased; compared with the experimental groups fed with the feed prepared in Example 1, in the experimental group fed with the feed prepared in Comparative Example 1, the diarrhea rate did not change significantly.

[0105] Generally speaking, compared with the experimental groups fed only with ordinary diets, adding the feed prepared in Examples 1 - 3 can significantly increase the weight gain of fattening pigs, reduce the feed conversion ratio and diarrhea rate; compared with the experimental groups fed with the feed prepared in Examples 1 - 3, the effects of adding the feed prepared in Comparative Examples 1 - 8 are poorer.

Claims

1. A method for preparing an anti-diarrhea feed using beer brewing waste, characterized in that, It includes the following steps: (1) Rinse beer waste yeast with water, take the supernatant after centrifugation, and add cyclodextrin to the supernatant to obtain a beer waste yeast washing solution - cyclodextrin solution; reserve a part of the washed beer waste yeast as a microbial agent; dry the remaining washed beer waste yeast to obtain beer waste yeast powder; (2) Weigh 3 - 4 parts of beer grains, 3 - 4 parts of corn kernels, 2 - 4 parts of soybean meal, 1 - 2 parts of beer waste yeast powder, 1 - 2 parts of rice bran, and 1 - 2 parts of wheat bran by weight, crush and mix them to obtain a fermentation raw material; (3) Inoculate the washed beer waste yeast microbial agent prepared in step (1), Bacillus subtilis, and Lactobacillus acidophilus into the fermentation raw material prepared in step (2); Then add the beer waste yeast washing solution - cyclodextrin solution prepared in step (1), mix well, and perform anaerobic solid fermentation; (4) After the fermentation ends, dry it to obtain an anti - diarrhea feed.

2. The method according to claim 1, characterized in that In step (1), rinse the beer waste yeast with 1 - 10 times the weight of sterile water or physiological saline to obtain a rinsing solution and the washed beer waste yeast; add cyclodextrin to the rinsing solution, and the added concentration of cyclodextrin is 0.5% - 5% of the rinsing solution by mass fraction, and stir at 200 - 280 rpm for 1 - 4 h; reserve the washed beer waste yeast as a microbial agent according to the specific dosage, and dry the remaining washed beer waste yeast at 55 - 70 °C to obtain yeast dry powder; Preferably, reserve the washed beer waste yeast according to the specific dosage and store it at 4 °C under sterile conditions as a microbial agent for later use.

3. The method according to claim 1, wherein In step (1), the rinsing method is to mix sterile water or physiological saline with beer waste yeast evenly, centrifuge at above 800 rpm to take the supernatant to obtain the rinsing solution, and the remaining is the washed beer waste yeast.

4. The method according to claim 1, characterized in that In step (1), the cyclodextrin is β - cyclodextrin and / or hydroxypropyl - β - cyclodextrin.

5. The method according to claim 1, characterized in that In step (2), after crushing and mixing, pass through a 40 - 80 - mesh sieve to obtain the fermentation raw material.

6. The method according to claim 1, characterized in that, In step (3), by weight, mix 10 - 30 parts of the washed beer waste yeast prepared in step (1), 1 - 3 parts of Bacillus subtilis, 1 - 3 parts of Lactobacillus acidophilus, 100 - 150 parts of the fermentation raw material, and 100 - 250 parts of the beer waste yeast washing solution - cyclodextrin solution, and perform anaerobic solid fermentation at 30 - 35 °C for 96 - 120 h.

7. The method according to claim 1, wherein Perform anaerobic solid fermentation in a sealed container with a breathing valve; Preferably, the sealed container with a breathing valve is a sealed bag with a breathing valve.

8. The method according to claim 1, wherein In step (4), the drying temperature is 30 - 78 °C.

9. Application of the anti - diarrhea feed prepared by the method according to any one of claims 1 - 8 in livestock breeding.

10. The application according to claim 9, characterized in that, The livestock is a pig; Preferably, the pig is a fattening pig.

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