Mouse eyeball protrusion measuring instrument

Abstract

The invention relates to the technical field of eyeball protrusion measurement, and discloses a mouse eyeball protrusion measuring instrument which comprises a bottom plate, a body fixing assembly, a measuring assembly and a head fixing assembly, the measuring assembly comprises a moving block, the moving block is arranged at the rear end of the body fixing assembly in a sliding mode, and a measuring groove is formed in the moving block; a first measuring block and a second measuring block which are bilaterally symmetrical are arranged at the opening end of the measuring groove, eye placing holes are formed in the front ends of the first measuring block and the second measuring block, the body fixing assembly and the head fixing assembly cooperate to stabilize the body position of a mouse, and the activity of the mouse is effectively limited; the first measuring block and the second measuring block which are symmetrically arranged are matched with the four sets of elastic reset assemblies, the contact pressure can be adjusted when the mouse eyeballs are in contact, uniform attachment is kept, the mouse eyeballs are prevented from being damaged due to excessive compression, and the mouse eyeball protrusion is measured through the front-back movement distance of the mouse eyeballs abutting against the first measuring block and the second measuring block. The operation process is simplified and the measurement precision is improved.

Description

Technical Field

[0001] The invention relates to the technical field of eyeball protrusion measurement, in particular to an eyeball protrusion measurement instrument for mice. Background Art

[0002] Thyroid-associated ophthalmopathy (TAO) is an inflammatory orbital disease closely related to autoimmune thyroid diseases (such as Graves' disease). Its typical clinical features include protruding eyes (exophthalmos), eyelid retraction, hypertrophy of the extraocular muscles, and optic neuropathy. In severe cases, it can lead to visual impairment or even blindness. The pathogenesis of TAO has not yet been fully elucidated. It is currently believed to be closely related to factors such as activation of orbital fibroblasts, autoimmune reactions mediated by inflammatory factors, glycosaminoglycan deposition, and adipose tissue hyperplasia.

[0003] In TAO research, animal models are core tools for exploring pathological mechanisms, evaluating therapeutic targets, and verifying intervention methods. Among them, mouse models are widely used because of their clear genetic background, convenient operation, and high similarity between the immune system and humans. Detecting the degree of mouse eyeball exophthalmos is a key indicator for evaluating the success of the TAO model and the progress of the research. By quantitatively detecting the degree of mouse eyeball exophthalmos, it can be verified whether the animal model truly simulates the core phenotype of human TAO. There are differences between mouse models and human TAO in anatomical structure and immune microenvironment, but the repeatable detection of the exophthalmos phenotype makes it a bridge between basic research and clinical application. By comparing the consistency of pathological features related to exophthalmos in mice and humans, the credibility of preclinical research results can be improved and the translation process of potential therapies can be accelerated.

[0004] The prior art often uses an eyeball protrusion measuring instrument to measure the protrusion of human eyeballs. For example, a Chinese patent with authorization announcement number CN216962422U discloses an eyeball protrusion measuring instrument. The patented technology includes a base, a bracket, a baffle, a measuring component, a head fixing component and a control component. The head fixing component is used to fix the head of the subject. The measuring element includes a track, a distance sensor, a light emitter and a first driving member. The control component controls the first driving member to drive the distance sensor to move on the track to automatically scan the subject's eyeball and identify the corneal vertex. The existing measuring instrument for human eyeball protrusion improves the measurement accuracy of human eyeball protrusion through automated scanning and head fixing design. However, the mouse eye socket is shallow and the eyeball volume is small. It is difficult for traditional distance sensors to accurately locate the mouse corneal vertex, and the mouse is highly active, resulting in inaccurate measurement results when used to measure the mouse model. Summary of the invention

[0005] The technical problems to be solved by the present invention are:

[0006] Existing measuring instruments for human proptosis make it difficult for traditional ranging sensors to accurately locate the corneal apex of mice, and the high activity of mice results in inaccurate measurement results when measuring mouse models.

[0007] To solve the above technical problems, the present invention provides a mouse proptosis measuring instrument, including a bottom plate and a measuring component. The bottom plate is provided with a body fixing component and a head fixing component;

[0008] The measuring component includes a moving block, which is slidably arranged at the front end of the body fixing component. A measuring groove is formed on the moving block;

[0009] An opening is provided at the rear end of the measuring groove. At the opening end of the measuring groove, there are symmetrically arranged first measuring blocks and second measuring blocks on the left and right. Eye placement holes are provided at the rear ends of the first measuring block and the second measuring block;

[0010] The first measuring block is slidably connected to the left side wall of the measuring groove in the front-back direction, and the second measuring block is slidably connected to the right side wall of the measuring groove in the front-back direction. A first elastic reset component is connected between the first measuring block and the left side wall, and a second elastic reset component is connected between the second measuring block and the right side wall. A third elastic reset component is connected between the front end of the first measuring block and the front side wall of the measuring groove, and a fourth elastic reset component is connected between the front end of the second measuring block and the front side wall of the measuring groove;

[0011] Pushing the moving block can make the two eyeballs of the mouse respectively fit against the edges of the corresponding eye placement holes.

[0012] Preferably,

[0013] The first elastic reset component includes a first compression spring and a first telescopic rod;

[0014] The second elastic reset component includes a second compression spring and a second telescopic rod;

[0015] One end of the first telescopic rod is slidably arranged on the left side wall of the measuring groove, and the other end of the first telescopic rod is fixedly connected to the left side wall of the first measuring block;

[0016] One end of the second telescopic rod is slidably arranged on the right side wall of the measuring groove, and the other end of the second telescopic rod is fixedly connected to the right side wall of the second measuring block;

[0017] The first compression spring and the second compression spring are respectively sleeved on the first telescopic rod and the second telescopic rod,

[0018] Or spring grooves extending axially are provided on both the first telescopic rod and the second telescopic rod. The first compression spring is installed in the spring groove on the first telescopic rod, and the second compression spring is installed in the spring groove on the second telescopic rod.

[0019] Preferably,

[0020] The third elastic reset component is a third compression spring, and the fourth elastic reset component is a fourth compression spring;

[0021] One end of the third compression spring is fixedly connected to the front side wall of the measurement groove, and the other end of the third compression spring is fixedly connected to the front side wall of the first measurement block;

[0022] One end of the fourth compression spring is fixedly connected to the front side wall of the measurement groove, and the other end of the fourth compression spring is fixedly connected to the front side wall of the second measurement block.

[0023] Preferably,

[0024] A first limiting groove is provided on the left side wall of the measurement groove, a first limiting block is slidably arranged in the first limiting groove, one end of the first telescopic rod is fixedly connected to the first limiting block, and the other end of the first telescopic rod is fixedly connected to the left side wall of the first measurement block;

[0025] A second limiting groove is provided on the right side wall of the measurement groove, a second limiting block is slidably arranged in the second limiting groove, one end of the second telescopic rod is fixedly connected to the second limiting block, and the other end of the second telescopic rod is fixedly connected to the right side wall of the second measurement block.

[0026] Preferably,

[0027] Scales extending in the front-back direction of the measurement groove are provided on both sides of the measurement groove.

[0028] Preferably,

[0029] A first installation groove extending in the front-back direction of the bottom plate is formed on the bottom plate, and the moving block is slidably arranged in the first installation groove.

[0030] Preferably,

[0031] Head fixing components are provided on both sides of the body fixing component, and second installation grooves extending in the left-right direction of the bottom plate are formed on both sides of the bottom plate;

[0032] Each head fixing component includes a fixing block and a head fixing rod. Each fixing block is slidably arranged in each second installation groove, and each head fixing rod is arranged on one side of each fixing block facing the body fixing component;

[0033] A locking member is further provided on each fixing block and / or the bottom plate, and the locking member is used to fix the position of each fixing block.

[0034] Preferably,

[0035] The locking member includes an overhanging plate overhanging on the side wall of the fixing block, a plug rod is installed on the overhanging plate, and a plurality of pin holes are spaced on the bottom plate, and the plug rod is used to be inserted into the pin holes;

[0036] A pulling block is fixed at the top of the insertion rod, and a connecting spring is connected between the pulling block and the overhanging plate.

[0037] The present application also provides a method for measuring the exophthalmos degree of a mouse using a mouse exophthalmos measuring instrument, including:

[0038] S1, Place the mouse on the body fixing component, use the body fixing component to fix the body of the mouse, and use the head fixing component to fix the head of the mouse;

[0039] S2, Record the horizontal position H1 of the rear end faces of the first measuring block and the second measuring block.

[0040] S3, Slide the moving block towards the body fixing component so that the two eyes of the mouse are respectively in contact with the first measuring block and the second measuring block. The eyes of the mouse press against the first measuring block and the second measuring block to move. When the eyes of the mouse are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block at this time, and record the horizontal position H3 of the rear end face of the second measuring block at this time;

[0041] S4, Calculate the difference ΔH1 between H2 and H1, and ΔH1 is the exophthalmos degree of the left eye of the mouse;

[0042] Calculate the difference ΔH2 between H3 and H1, and ΔH2 is the exophthalmos degree of the right eye of the mouse.

[0043] Preferably,

[0044] S3, Slide the moving block towards the body fixing component so that the two eyes of the mouse are respectively in contact with the first measuring block and the second measuring block. The eyes of the mouse press against the first measuring block and the second measuring block to move. When the eyes of the mouse are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block at this time, and record the horizontal position H3 of the rear end face of the second measuring block at this time;

[0045] Specifically: When sliding the moving block towards the body fixing component, the two eyes of the mouse first come into contact with the first measuring block and the second measuring block respectively. Continuing to slide the moving block, the left eye of the mouse causes the first measuring block to slide to the left and forward, and the right eye of the mouse causes the second measuring block to slide to the right and forward.

[0046] Compared with the prior art, the mouse exophthalmos measuring instrument provided by the embodiment of the present invention has the following beneficial effects:

[0047] The body fixation component and the head fixation component cooperate to stabilize the posture of the mouse, effectively restrict its mobility, and ensure the stability of the eye positioning during measurement. The symmetrically arranged first measurement block and second measurement block cooperate with four groups of elastic reset components, which can adjust the contact pressure and maintain uniform fitting when contacting the mouse's eyes, avoiding excessive compression and damage to the mouse's eyes. The protrusion of the mouse's eyes is measured by the forward and backward movement distances of the first measurement block and the second measurement block pressed by the mouse's eyes, simplifying the operation process, improving the measurement accuracy and repeatability, and meeting the dynamic monitoring requirements of the in vivo mouse model. Description of the Drawings

[0048] Figure 1 is the front view structural schematic diagram of a mouse eye protrusion measuring instrument provided by an embodiment of the present invention;

[0049] Figure 2 is the structural schematic diagram of the measurement component of a mouse eye protrusion measuring instrument provided by an embodiment of the present invention;

[0050] Figure 3 is the structural schematic diagram of the head fixation component of a mouse eye protrusion measuring instrument provided by an embodiment of the present invention;

[0051] Figure 4 is Figure 1 the enlarged view of part A;

[0052] Figure 5 is the schematic diagram of measurement after placing the mouse of a mouse eye protrusion measuring instrument provided by an embodiment of the present invention.

[0053] In the figure, 1, bottom plate; 2, body fixation component; 201, elastic band; 3, measurement component; 301, first installation groove; 302, moving block; 303, scale line; 304, measurement groove; 3051, first measurement block; 3052, second measurement block; 306, eye placement hole; 3071, first limit groove; 3072, second limit groove; 3081, first limit block; 3082, second limit block; 3091, first compression spring; 3092, second compression spring; 3101, first telescopic rod; 3102, second telescopic rod; 3111, third compression spring; 3112, fourth compression spring; 4, head fixation component; 401, second installation groove; 402, slide rail; 403, sliding block; 404, fixed block; 405, head fixation rod; 406, overhanging plate; 407, insertion rod; 408, pulling block; 409, pin hole; 410, connecting spring. Detailed Embodiments

[0054] The following combines the drawings and embodiments to further describe in detail the specific embodiments of the present invention. The following embodiments are used to illustrate the present invention, but are not used to limit the scope of the present invention.

[0055] As Figures 1 to 5 shown, a mouse exophthalmos measuring instrument according to a preferred embodiment of the present invention includes a bottom plate 1. A body fixing assembly 2 is fixedly connected to the front end of the top of the bottom plate 1. The body fixing assembly 2 is used to fix the body of the mouse. A measuring assembly 3 is arranged at the front end of the body fixing assembly 2. Head fixing assemblies 4 are arranged on both sides of the body fixing assembly 2. The head fixing assemblies 4 are used to fix the head of the mouse.

[0056] Specifically, the body fixing assembly 2 includes a body fixing block. The body fixing block is fixed on the bottom plate 1. A body placement groove is provided on the top of the body fixing block. Elastic straps 201 are installed on the groove wall of the body placement groove. The staff places the mouse in the body placement groove, and then fixes the body of the mouse through the elastic straps 201, effectively preventing movement and ensuring stability and safety during the experiment.

[0057] Specifically, a first installation groove 301 extending forward and backward is formed on the bottom plate;

[0058] The measuring assembly 3 includes a moving block 302. The bottom of the moving block 302 is slidably arranged in the first installation groove 301, so as to realize the sliding connection of the moving block 302;

[0059] Specifically, a measuring groove 304 is formed on the top of the moving block 302. An opening is provided at the rear end of the measuring groove 304. Symmetric first measuring blocks 3051 and second measuring blocks 3052 are provided at the opening end of the measuring groove 304. Eye placement holes 306 are provided at the rear ends of the first measuring block 3051 and the second measuring block 3052;

[0060] The first measuring block 3051 is slidably connected to the left side wall of the measuring groove 304 in the front-back direction. The second measuring block 3052 is slidably connected to the right side wall of the measuring groove 304 in the front-back direction. A first elastic reset assembly is connected between the first measuring block 3051 and the left side wall. A second elastic reset assembly is connected between the second measuring block 3052 and the right side wall. A third elastic reset assembly is connected between the front end of the first measuring block 3051 and the front side wall of the measuring groove 304. A fourth elastic reset assembly is connected between the front end of the second measuring block 3052 and the front side wall of the measuring groove 304; After fixing the mouse, push the moving block 302 to slide towards the body fixing assembly 2 until the two eyeballs of the mouse respectively press against the first measuring block 3051 and the second measuring block 3052. When the two eyeballs of the mouse are both in contact with the edges of the respective eye placement holes 306, record the distances that the first measuring block 3051 and the second measuring block 3052 move backward.

[0061] The multi-directional elastic self-adaptive adjustment of the measurement block is realized through four groups of elastic reset components, dynamically balancing the contact pressure of the mouse eyeball. The exophthalmos of the mouse eyeball is measured by the front and back moving distances of the first measurement block and the second measurement block pressed by the mouse eyeball, simplifying the operation process, improving the measurement accuracy and repeatability, and meeting the dynamic monitoring requirements of the in-vivo mouse model.

[0062] Specifically, a first inclined surface is provided at the front end of the first measurement block, and a second inclined surface is provided at the front end of the second measurement block. The eye placement holes are respectively arranged on the first inclined surface and the second inclined surface of the first measurement block and the second measurement block.

[0063] Specifically, the first measurement block is in the shape of a cuboid, and a corner is cut off at the right front end of the first measurement block to form a pentagonal prism, and the first inclined surface is formed at the right front end of the pentagonal prism; the second measurement block is in the shape of a cuboid, and a corner is cut off at the left front end of the second measurement block to form a pentagonal prism, and the second inclined surface is formed at the left front of the pentagonal prism.

[0064] The first and second inclined surfaces are respectively adapted to the physiological curvatures of the left and right eyeballs of the mouse by an asymmetric geometric design, and a progressive fit is formed when contacting the eyeball through the guidance of the inclined surface, avoiding mechanical damage to the eyeball by the acute-angle edge; the eye placement holes arranged on the inclined surface form a directional limiting relationship with the corneal apex. During the process of the measurement block being pushed, the inclined surface decomposes the eyeball pressing force into a horizontal displacement driving force and a vertical buffering force, which not only ensures that the eyeball is accurately guided into the hole along the preset path to achieve stable positioning, but also optimizes the dispersion of the contact pressure through the inclination angle of the inclined surface, synchronously realizing eyeball protection and linear conversion of displacement data in dynamic measurement, and significantly improving the operability and result reliability of measuring the exophthalmos of the in-vivo mouse eyeball.

[0065] Specifically, the first elastic reset component includes a first compression spring 3091 and a first telescopic rod 3101; the second elastic reset component includes a second compression spring 3092 and a second telescopic rod 3102; one end of the first telescopic rod 3101 is slidably arranged on the left side wall of the measurement groove 304, and the other end of the first telescopic rod 3101 is fixedly connected to the left side wall of the first measurement block 3051; one end of the second telescopic rod 3102 is slidably arranged on the right side wall of the measurement groove 304, and the other end of the second telescopic rod 3102 is fixedly connected to the right side wall of the second measurement block 3052; the first compression spring 3091 and the second compression spring 3092 are respectively sleeved on the first telescopic rod 3101 and the second telescopic rod 3102.

[0066] Through the collaborative design of the first and second telescopic rods and the compression spring, the linear sliding and guiding function of the telescopic rods is used to constrain the movement trajectory of the measuring block, ensuring the displacement stability of the first and second measuring blocks in the left-right direction; the compression spring sleeved on the telescopic rod provides an elastic restoring force, enabling the measuring block to adaptively adjust the contact distance according to the size of the mouse eyeball, avoiding eye damage caused by rigid compression and eliminating interference of the mouse's minute movements on the measurement reference through elastic buffering; at the same time, the combined design of linear guiding and elastic reset converts the displacement of the eye apex into linearly quantifiable data, significantly improving the repeatability and accuracy of the measurement results and meeting the measurement requirements of mice of different body sizes.

[0067] Specifically,

[0068] The third elastic reset component is the third compression spring 3111, and the fourth elastic reset component is the fourth compression spring 3112; one end of the third compression spring 3111 is fixedly connected to the front side wall of the measurement groove 304, and the other end of the third compression spring 3111 is fixedly connected to the front side wall of the first measuring block 3051; one end of the fourth compression spring 3112 is fixedly connected to the front side wall of the measurement groove 304, and the other end of the fourth compression spring 3112 is fixedly connected to the front side wall of the second measuring block 3052.

[0069] When the measuring block is pushed by an external force to contact the mouse eyeball, the compression spring generates linear compression and stores energy, dynamically balancing the operating thrust through elastic resistance to prevent the measuring block from overly pressing the eyeball and causing damage; after the measurement, the compression spring releases the stored elastic potential energy, automatically driving the measuring block to quickly reset to the initial position along a straight path, eliminating the operation error of manual pulling back.

[0070] Specifically,

[0071] A first limiting groove 3071 is provided on the left side wall of the measurement groove 304, a first limiting block 3081 is slidably arranged in the first limiting groove 3071, one end of the first telescopic rod 3101 is fixedly connected to the first limiting block 3081, and the other end of the first telescopic rod 3101 is fixedly connected to the left side wall of the first measuring block 3051;

[0072] A second limiting groove 3072 is provided on the right side wall of the measurement groove 304, a second limiting block 3082 is slidably arranged in the second limiting groove 3072, one end of the second telescopic rod 3102 is fixedly connected to the second limiting block 3082, and the other end of the second telescopic rod 3102 is fixedly connected to the right side wall of the second measuring block 3052.

[0073] Scales extending in the front-rear direction of the measurement groove 304 are provided on both sides of the measurement groove 304, and the scales are provided to facilitate the staff to record the moving distances of the first measuring block and the second measuring block.

[0074] Refer toFigure 1 , Figure 3 and Figure 4 as shown, specifically,

[0075] Second mounting grooves 401 extending in the left - right direction of the base plate 1 are provided on both sides of the base plate 1;

[0076] Each head fixing assembly 4 includes a fixing block 404 and a head fixing rod 405. Each fixing block 404 is respectively slidably disposed in each second mounting groove 401, and each head fixing rod 405 is respectively disposed on one side of the top of each fixing block 404 facing the body fixing assembly 2;

[0077] A locking member is further provided on each fixing block 404 and / or the base plate 1, and the locking member is used to fix the position of each fixing block 404.

[0078] Specifically,

[0079] Sliders 403 are fixedly connected to the bottoms of each fixing block 404. Slide rails 402 are provided at the front end and the rear end of the inner cavity of each second mounting groove 401. The inner cavity of the slide rail 402 is slidably connected to the surface of the slider 403. The staff can push the two fixing blocks 404 inward, and the slider 403 moves in the second mounting groove 401. At the same time, head fixing rods 405 are fixedly connected to the facing surfaces of the fixing blocks 404, so that the head fixing rods 405 fix the head of the mouse, so that when the staff measures the proptosis of the mouse's eyeball, it can be ensured that the head of the mouse will not shake, which is convenient for the staff to measure better.

[0080] Specifically,

[0081] The locking member includes a cantilever plate 406 extending from the side wall of the fixing block 404. A plug rod 407 is slidably mounted on the cantilever plate 406. A plurality of pin holes 409 are provided on the surface of the base plate 1. The surface of the plug rod 407 is inserted into the interior of the pin hole 409. When the staff finishes fixing the head of the mouse, the plug rod 407 can be inserted into the pin hole 409 at the top of the base plate 1 to fix the fixing block 404.

[0082] Specifically,

[0083] A pull block 408 is fixed to the top of the insertion rod 407. A connecting spring 410 is connected between the pull block 408 and the overhanging plate 406. One end of the connecting spring 410 is fixedly connected to the bottom of the pull block 408, and the other end of the connecting spring 410 is fixedly connected to the top of the overhanging plate 406. When the staff pulls the pull block 408, the connecting spring 410 at the bottom of the pull block 408 will store energy outward. When the staff releases the pull block 408, the connecting spring 410 will drive the insertion rod 407 connected to the pull block 408 to rebound. At the same time, the connecting spring 410 also plays a connecting role, making it more convenient for the staff during use. The outer diameter of the insertion rod 407 is slidably connected to the inner cavity of the pin hole 409, and the outer diameter of the insertion rod 407 is adapted to the inner diameter of the pin hole 409. Because the outer diameter of the insertion rod 407 is adapted to the inner diameter of the pin hole 409, when the insertion rod 407 is inserted, a firm limit is formed between the surface of the insertion rod 407 and the inside of the pin hole 409, so that the fixing block 404 will not shake and is more stable when the insertion rod 407 is inserted into the pin hole 409.

[0084] In other embodiments, the first elastic reset component only includes a first compression spring, and the second elastic reset component only includes a second compression spring.

[0085] In other embodiments, the locking member includes magnets provided at the bottoms of the sliders and magnetic strips extending left and right in each second installation groove 401.

[0086] The present application also provides a method for measuring the protrusion degree of a mouse eyeball of a mouse eyeball protrusion measuring instrument, including:

[0087] S1, place the mouse on the body fixing component 2, use the body fixing component 2 to fix the body of the mouse, and use the head fixing component 4 to fix the head of the mouse;

[0088] S2, record the horizontal position H1 of the rear end faces of the first measuring block 3051 and the second measuring block 3052;

[0089] S3, slide the moving block 302 towards the body fixing component 2 so that the two eyeballs of the mouse are respectively in contact with the first measuring block 3051 and the second measuring block 3052. The eyeballs of the mouse press the first measuring block 3051 and the second measuring block 3052 to move. When the eyeballs of the mouse are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block 3051 at this time, and record the horizontal position H3 of the rear end face of the second measuring block 3052 at this time;

[0090] S4, calculate the difference ΔH1 between H2 and H1, and ΔH1 is the protrusion degree of the left eyeball of the mouse;

[0091] Calculate the difference ΔH2 between H3 and H1, and ΔH2 is the protrusion degree of the right eyeball of the mouse.

[0092] Specifically,

[0093] S3. Slide the moving block 302 towards the body fixing component 2, so that the two eyeballs of the mouse are respectively in contact with the first measuring block 3051 and the second measuring block 3052. The eyeballs of the mouse press against the first measuring block 3051 and the second measuring block 3052 to move. When the eyeballs of the mouse are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block 3051 at this time, and record the horizontal position H3 of the rear end face of the second measuring block 3052 at this time;

[0094] Specifically, when sliding the moving block 302 towards the body fixing component 2, the two eyeballs of the mouse first come into contact with the first measuring block 3051 and the second measuring block 3052 respectively. Continue to slide the moving block 302. The left eyeball of the mouse causes the first measuring block 3051 to slide leftward and forward, and the right eyeball of the mouse causes the second measuring block 3052 to slide rightward and forward.

[0095] The usage process of the present invention is as follows: Place the mouse on the body fixing component 2. The staff fixes the mouse's body through the elastic strap 201 and pushes the two fixing blocks 404 inward. The bottom of the fixing block 404 is connected to the sliding block 403, and the sliding block 403 moves in the second installation groove 401. At the same time, the opposite surfaces of the fixing block 404 are fixedly connected to the head fixing rods 405, so that the head fixing rods 405 fix the mouse's head, so that when the staff measures the proptosis of the mouse's eyeball, it can be ensured that the mouse's head will not shake. The fixing block 404 can be fixed by inserting the insertion rod 407 into the pin hole 409 at the top of the bottom plate 1, so that the staff can better measure the proptosis of the mouse's eyeball. When the staff pulls the pull block 408, the connecting spring 410 at the bottom of the pull block 408 will store energy outward. When the staff releases the pull block 408, the connecting spring 410 will drive the insertion rod 407 connected to the pull block 408 to rebound. At the same time, the connecting spring 410 also plays a connecting role, so that the staff can be more efficient during use. Because the outer diameter of the insertion rod 407 is adapted to the inner diameter of the pin hole 409, when the insertion rod 407 is inserted, a stable limit is formed between the surface of the insertion rod 407 and the inside of the pin hole 409, so that when the insertion rod 407 is inserted into the pin hole 409, the fixing block 404 will not shake and is more stable. Then, push the moving block 302 backward so that the moving block 302 slides inside the first installation groove 301. The top of the moving block 302 is provided with a first sliding groove 304. At the same time, the open end of the first sliding groove 304 is slidably connected with a first measuring block 3051 and a second measuring block 3052. Push the moving block 302 so that the two eyeballs of the mouse are in contact with the first measuring block 3051 and the second measuring block 3052 respectively. The mouse's eyeballs press the first measuring block 3051 and the second measuring block 3052 to move. The left eyeball of the mouse is respectively on the first inclined surface of the first measuring block 3051, and the right eyeball of the mouse presses on the second inclined surface of the second measuring block 3052. When the moving block 302 is moved continuously, the left eyeball of the mouse causes the first measuring block 3051 to slide leftward and forward, and the right eyeball of the mouse causes the second measuring block 3052 to slide rightward and forward, so that the measurement can be carried out according to the size of different mouse heads. When the mouse's eyeballs are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the distance that the rear end face of the first measuring block 3051 moves forward, and record the distance that the rear end face of the second measuring block 3052 moves forward, which is the proptosis of the two eyeballs of the mouse.

[0096] In summary, the embodiment of the present invention provides a mouse exophthalmos measuring instrument. Through the coordinated action of the body fixing component and the head fixing component, the posture of the mouse is stabilized, its mobility is effectively restricted, and the stability of the eye positioning during measurement is ensured. The symmetrically arranged first measuring block and the second measuring block cooperate with four groups of elastic reset components, which can adjust the contact pressure and maintain uniform fitting when contacting the mouse's eyes, avoiding excessive compression damage to the mouse's eyes. The exophthalmos of the mouse is measured by the forward and backward movement distances of the first measuring block and the second measuring block pressed by the mouse's eyes, simplifying the operation process, improving the measurement accuracy and repeatability, and meeting the dynamic monitoring requirements of the in vivo mouse model.

[0097] The above are only the preferred embodiments of the present invention. It should be noted that for those of ordinary skill in the art of the present technology, without departing from the technical principle of the present invention, several improvements and substitutions can be made, and these improvements and substitutions should also be regarded as the protection scope of the present invention.

Claims

1. A mouse exophthalmos measuring instrument, characterized in that: It includes a bottom plate (1) and a measurement assembly (3), and a body fixing assembly (2) and a head fixing assembly (4) are provided on the bottom plate; The measurement assembly (3) includes a moving block (302), and the moving block (302) is slidably arranged at the front end of the body fixing assembly (2), and a measurement groove (304) is formed on the moving block (302); An opening is provided at the rear end of the measurement groove (304), and symmetric first measurement blocks (3051) and second measurement blocks (3052) are provided at the opening end of the measurement groove (304), and eye placement holes (306) are provided at the rear ends of both the first measurement block (3051) and the second measurement block (3052); The first measurement block (3051) is slidably connected to the left side wall of the measurement groove (304) in the front-back direction, the second measurement block (3052) is slidably connected to the right side wall of the measurement groove (304) in the front-back direction, a first elastic reset assembly is connected between the first measurement block (3051) and the left side wall, a second elastic reset assembly is connected between the second measurement block (3052) and the right side wall, a third elastic reset assembly is connected between the front end of the first measurement block (3051) and the front side wall of the measurement groove (304), and a fourth elastic reset assembly is connected between the front end of the second measurement block (3052) and the front side wall of the measurement groove (304); Pushing the moving block (302) can make the two eyeballs of the mouse respectively fit against the edges of the corresponding eye placement holes (306).

2. The mouse eyeball protrusion measuring instrument according to claim 1, wherein: The first elastic reset assembly includes a first compression spring (3091) and a first telescopic rod (3101); The second elastic reset assembly includes a second compression spring (3092) and a second telescopic rod (3102); One end of the first telescopic rod (3101) is slidably arranged on the left side wall of the measurement groove (304), and the other end of the first telescopic rod (3101) is fixedly connected to the left side wall of the first measurement block (3051); One end of the second telescopic rod (3102) is slidably arranged on the right side wall of the measurement groove (304), and the other end of the second telescopic rod (3102) is fixedly connected to the right side wall of the second measurement block (3052); The first compression spring (3091) and the second compression spring (3092) are respectively sleeved on the first telescopic rod (3101) and the second telescopic rod (3102), or axial spring grooves are provided on both the first telescopic rod (3101) and the second telescopic rod (3102), the first compression spring (3091) is installed in the spring groove on the first telescopic rod (3101), and the second compression spring (3092) is installed in the spring groove on the second telescopic rod (3102).

3. The mouse eyeball protrusion measuring instrument according to claim 1 or 2, wherein: The third elastic reset component is a third compression spring (3111), and the fourth elastic reset component is a fourth compression spring (3112); One end of the third compression spring (3111) is fixedly connected to the front side wall of the measurement groove (304), and the other end of the third compression spring (3111) is fixedly connected to the front side wall of the first measurement block (3051); One end of the fourth compression spring (3112) is fixedly connected to the front side wall of the measurement groove (304), and the other end of the fourth compression spring (3112) is fixedly connected to the front side wall of the second measurement block (3052).

4. The mouse eyeball protrusion measuring instrument according to claim 2, wherein: A first limiting groove (3071) is provided on the left side wall of the measurement groove (304), a first limiting block (3081) is slidably arranged in the first limiting groove (3071), one end of the first telescopic rod (3101) is fixedly connected to the first limiting block (3081), and the other end of the first telescopic rod (3101) is fixedly connected to the left side wall of the first measurement block (3051); A second limiting groove (3072) is provided on the right side wall of the measurement groove (304), a second limiting block (3082) is slidably arranged in the second limiting groove (3072), one end of the second telescopic rod (3102) is fixedly connected to the second limiting block (3082), and the other end of the second telescopic rod (3102) is fixedly connected to the right side wall of the second measurement block (3052).

5. The mouse eyeball protrusion measuring instrument according to claim 1, wherein: Scales extending in the front-rear direction of the measurement groove (304) are provided on both sides of the measurement groove (304).

6. The mouse eyeball protrusion measuring instrument according to claim 1, wherein: A first installation groove (301) extending in the front-rear direction of the bottom plate (1) is formed on the bottom plate (1), and the moving block (302) is slidably arranged in the first installation groove (301).

7. The mouse eyeball protrusion measuring instrument according to claim 1, wherein: The head fixing components (4) are provided on both sides of the body fixing component (2), and second installation grooves (401) extending in the left-right direction of the bottom plate (1) are formed on both sides of the bottom plate (1); Each of the head fixing components (4) includes a fixing block (404) and a head fixing rod (405), each of the fixing blocks (404) is respectively slidably arranged in each of the second installation grooves (401), and each of the head fixing rods (405) is respectively arranged on one side of the top of each of the fixing blocks (404) facing the body fixing component (2); A locking member is further provided on each of the fixing blocks (404) and / or the bottom plate (1), and the locking member is used to fix the positions of each of the fixing blocks (404).

8. The mouse eyeball protrusion measuring instrument according to claim 7, wherein: The locking member includes a cantilever plate (406) cantilevered on the side wall of the fixed block (404). A plug rod (407) is installed on the cantilever plate (406). A plurality of pin holes (409) are arranged on the bottom plate (1) at intervals. The plug rod (407) is used to be inserted into the pin holes (409). A pull block (408) is fixed at the top of the plug rod (407). A connecting spring (410) is connected between the pull block (408) and the cantilever plate (406).

9. The method for measuring the mouse eyeball protrusion degree of a mouse eyeball protrusion degree measuring instrument according to any one of claims 1-8, characterized in that: S1, Place the mouse on the body fixing component (2), use the body fixing component (2) to fix the body of the mouse, and use the head fixing component (4) to fix the head of the mouse; S2, Record the horizontal position H1 of the rear end faces of the first measuring block (3051) and the second measuring block (3052). S3, Slide the moving block (302) towards the body fixing component (2) so that the two eyeballs of the mouse are respectively in contact with the first measuring block (3051) and the second measuring block (3052). The mouse eyeballs press the first measuring block (3051) and the second measuring block (3052) to move. When the mouse eyeballs are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block (3051) at this time, and record the horizontal position H3 of the rear end face of the second measuring block (3052) at this time; S4, Calculate the difference ΔH1 between H2 and H1. ΔH1 is the protrusion degree of the left eyeball of the mouse; Calculate the difference ΔH2 between H3 and H1. ΔH2 is the protrusion degree of the right eyeball of the mouse.

10. The method for measuring the mouse eyeball protrusion degree according to claim 9, characterized in that: S3, Slide the moving block (302) towards the body fixing component (2) so that the two eyeballs of the mouse are respectively in contact with the first measuring block (3051) and the second measuring block (3052). The mouse eyeballs press the first measuring block (3051) and the second measuring block (3052) to move. When the mouse eyeballs are in contact with the edges of the eye placement grooves on the two measuring blocks, stop moving the measuring blocks, record the horizontal position H2 of the rear end face of the first measuring block (3051) at this time, and record the horizontal position H3 of the rear end face of the second measuring block (3052) at this time; Specifically: When sliding the moving block (302) towards the body fixing component (2), the two eyeballs of the mouse first come into contact with the first measuring block (3051) and the second measuring block (3052) respectively. Continue to slide the moving block (302). The left eyeball of the mouse causes the first measuring block (3051) to slide leftward and forward, and the right eyeball of the mouse causes the second measuring block (3052) to slide rightward and forward.

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