Cleaning separation device and method of use thereof

Abstract

The application discloses a cleaning separation device and a use method thereof, belongs to the field of chemiluminescence immunoassay, and relates to a mounting frame, a cleaning needle assembly, a gripper assembly, a cleaning station assembly, a magnetic separation assembly and a mixing assembly. The mounting frame comprises a vertical plate and a horizontal plate fixed to the vertical plate. The cleaning needle assembly and the gripper assembly are in transmission connection with the horizontal plate. The cleaning station assembly, the magnetic separation assembly and the mixing assembly are fixed to the horizontal plate and located on the same straight line. The mounting column comprises an end portion. The diameter of the adjusting hole of the horizontal plate is larger than the diameter of the end portion. The end portion is located in the adjusting hole and can adjust the position so that the position of the mounting frame relative to the mounting column can be adjusted. The positioning sheet is fixed to the mounting column and the mounting frame. The cleaning station assembly, the magnetic separation assembly and the mixing assembly can be integrally installed and disassembled. The cleaning station assembly, the magnetic separation assembly and the mixing assembly are located on the same straight line, and the gripper assembly is convenient to move.

Description

Technical Field

[0001] This invention relates to the field of chemiluminescence immunoassay, and in particular to a cleaning and separation apparatus and its usage. Background Technology

[0002] Chemiluminescent immunoassay combines a chemical reaction system with the immune system to detect antigens or antibodies. Labeling antigens and antibodies with chemiluminescent substances combines the specificity of an immune response with the high sensitivity of a chemiluminescent reaction. The procedure is simple, convenient, reproducible, and pollution-free, and is widely used in clinical practice.

[0003] Because the fully automated chemiluminescence immunoassay analyzer uses a fully automated control method to realize the immunobiochemical reaction process outside the human body and to detect and quantitatively analyze the results of the reaction process, the reaction cup needs to be cleaned, separated, and mixed during the reaction process. Therefore, corresponding components are required. The existing cleaning and separation device has an unreasonable structural design, which leads to complex component installation and difficult disassembly. In addition, the movement path of the translational components is unreasonable, resulting in long cleaning and separation process time, low efficiency, and difficulty in increasing throughput. Summary of the Invention

[0004] In order to overcome the shortcomings of the prior art, one of the objectives of this invention is to provide a cleaning and separation device that is simple in structure, easy to install, and highly efficient.

[0005] In order to overcome the shortcomings of the prior art, the second objective of this invention is to provide a method for using a cleaning and separation device that is simple in structure, easy to install, and highly efficient.

[0006] One of the objectives of this invention is achieved through the following technical solution:

[0007] A cleaning and separation device includes a mounting frame, which includes a vertical plate and a horizontal plate fixed to the vertical plate. The device also includes a mounting column, a positioning plate, a cleaning needle assembly, a gripper assembly, a cleaning station assembly, a magnetic separation assembly, a mixing assembly, and a translation drive assembly. The translation drive assembly includes a translation drive component, a rotating shaft, and a belt. The cleaning needle assembly and the gripper assembly are connected to the translation drive component. The cleaning station assembly, the magnetic separation assembly, and the mixing assembly are fixed to the horizontal plate and located on the same straight line. The magnetic separation assembly is located between the cleaning station assembly and the mixing assembly. The mounting column includes an end portion. The horizontal plate has an adjustment hole with a diameter larger than the diameter of the end portion. The end portion is located within the adjustment hole and its position can be adjusted so that the position of the mounting frame relative to the mounting column can be adjusted. The positioning plate fixes the mounting column and the mounting frame.

[0008] Furthermore, the horizontal plate is also provided with fixing holes, the positioning piece is provided with locking holes and positioning grooves, the locking holes are fixed to the end of the mounting column, the position of the positioning groove corresponds to the fixing holes, and the screw passes through the fixing holes and extends into the positioning groove and cooperates with the nut to fix the mounting column and the mounting bracket.

[0009] Furthermore, the translation drive is fixed to the vertical plate, there are multiple rotating shafts, the multiple rotating shafts are rotatably mounted on the vertical plate, the belt is sleeved on the rotating shaft, the output end of the translation drive is fixedly connected to one of the rotating shafts, and the cleaning needle assembly and the gripper assembly are connected to the belt.

[0010] Furthermore, the cleaning needle assembly includes a base, a bearing, and a cleaning needle. The base is mounted on the vertical plate, the bearing is installed inside the base, and the cleaning needle is rotatably mounted on the base via the bearing.

[0011] Furthermore, the cleaning needle assembly also includes a vertical drive and a rack. The output end of the vertical drive is equipped with a gear, and the vertical gear meshes with the rack to drive the base to move in the vertical direction.

[0012] Furthermore, the magnetic separation assembly includes a separation base and a magnet, the separation base being fixed to the horizontal plate, and the magnet being installed inside the separation base.

[0013] Furthermore, the number of magnets is four, the four magnets are arranged in a cross shape, and the two magnets located on the same straight line have the same magnetism, and the space between the four magnets forms a space for containing the reaction cup.

[0014] Furthermore, the mixing assembly includes a mixing motor, a mixing base, and a mixing head. The mixing motor is fixed to the horizontal plate, the mixing base is installed at the output end of the mixing motor, the mixing motor drives the mixing base to rotate, and the mixing head is installed on the mixing base. The mixing head is used to install the reaction cup.

[0015] Furthermore, the axis of the mixing head forms an angle with the axis of the mixing base.

[0016] The second objective of this invention is achieved by the following technical solution:

[0017] The method of using any of the above-mentioned cleaning and separation devices includes the following steps:

[0018] Install the mounting bracket onto the mounting post, adjust the position of the mounting bracket, and lock the mounting bracket to the mounting post using the positioning plate;

[0019] The translation drive component drives the gripper component to translate to the incubation tray, grabs the reaction cup on the incubation tray, and moves the reaction cup to the magnetic separation component;

[0020] The magnet attracts the magnet in the reaction cup, the liquid suction needle of the cleaning needle assembly draws up the liquid in the reaction cup, and the dispensing needle of the cleaning needle assembly adds buffer solution to the reaction cup to achieve the cleaning of the reactants in the reaction cup.

[0021] The gripper assembly grabs the reaction cup on the magnetic separation assembly and moves it to the mixing assembly. The mixing motor drives the mixing base to rotate, and the reactants in the reaction cup are mixed.

[0022] The gripper assembly picks up the reaction cup from the mixing assembly and places it into the incubation tray, where the incubation tray incubates the reactants in the reaction cup.

[0023] Once the cleaning needle assembly completes one cycle, it moves to the cleaning station for cleaning.

[0024] Compared with existing technologies, the cleaning and separation device of the present invention has the following advantages:

[0025] (1) The mounting column includes an end, and the horizontal plate is provided with an adjustment hole. The diameter of the adjustment hole is larger than the diameter of the end. The end is located in the adjustment hole and can be adjusted so that the position of the mounting bracket relative to the mounting column can be adjusted. The positioning piece fixes the mounting column and the mounting bracket.

[0026] (2) The cleaning station assembly, magnetic separation assembly and mixing assembly are fixed on the horizontal plate and located on the same straight line, so that the cleaning station assembly, magnetic separation assembly and mixing assembly can be installed and disassembled as a whole. The cleaning station assembly, magnetic separation assembly and mixing assembly are located on a straight line, which facilitates the movement of the gripper assembly.

[0027] (3) The cleaning needle is mounted on the base by rotating through the bearing. The cleaning needle is connected to the pipeline. When the pipeline is looping, the cleaning needle rotates to prevent the pipeline from looping.

[0028] (4) The four magnets are arranged in a cross shape, and the two magnets located on the same straight line have the same magnetism, so that the magnets can better attract the magnetic beads in the reaction cup;

[0029] (5) The axis of the mixing head forms an angle with the axis of the mixing base, so that when the mixing base rotates, the reactants in the reaction cup inside the mixing head are mixed more evenly.

[0030] (6) Since the cleaning needle assembly and gripper assembly require less time to complete a sample cycle, while the magnetic separation assembly and mixing assembly require more time, increasing the number of magnetic separation and mixing assemblies allows the use of one gripper assembly and one needle assembly, along with two or more sets of magnetic separation and mixing assemblies; this integrates the time cycles occupied by different components in the cleaning and separation device, reduces the average required time, improves the efficiency of needle and gripper use, and increases the test throughput of the entire device. Attached Figure Description

[0031] Figure 1 This is a perspective view of the cleaning and separation device of the present invention;

[0032] Figure 2 for Figure 1 A perspective view of the mounting frame for the cleaning and separation device;

[0033] Figure 3 for Figure 2 Another perspective view of the mounting bracket;

[0034] Figure 4 for Figure 1 A perspective view of a partial structure of the cleaning and separation device;

[0035] Figure 5 for Figure 1 Front view of the cleaning and separation device;

[0036] Figure 6 for Figure 1 A schematic diagram of the magnetic separation component of the cleaning and separation device;

[0037] Figure 7 This is a flowchart of the odd-numbered cycles of the cleaning and separation device of the present invention.

[0038] Figure 8 This is a flowchart of an even-numbered cycle of the cleaning and separation device of the present invention.

[0039] In the diagram: 21. Mounting column; 210. End; 22. Mounting bracket; 220. Vertical plate; 221. Horizontal plate; 2210. Adjustment hole; 2211. Fixing hole; 23. Positioning plate; 230. Positioning groove; 231. Locking hole; 24. Cleaning needle assembly; 240. Vertical drive component; 241. Rack; 242. Base; 243. Bearing; 244. Cleaning needle; 25. Grip assembly; 26. Cleaning station assembly; 27. Magnetic separator 270. Separation component; 271. Separation base; 272. Magnet; 273. First magnetic separation component; 274. Second magnetic separation component; 28. Mixing component; 280. Mixing motor; 281. Mixing base; 282. Mixing head; 283. First mixing component; 284. Second mixing component; 29. ​​Translation drive component; 290. Translation drive element; 291. Rotating shaft; 292. Belt; 100. Reaction cup. Detailed Implementation

[0040] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0041] It should be noted that when a component is said to be "fixed to" another component, it can be directly on the other component or it can be fixed through another intermediate component. When a component is said to be "connected to" another component, it can be directly connected to the other component or it may be fixed through another intermediate component. When a component is said to be "set on" another component, it can be set directly on the other component or it may be set through another intermediate component. The terms "vertical," "horizontal," "left," "right," and similar expressions used in this document are for illustrative purposes only.

[0042] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. The terminology used herein in the description of the invention is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and / or" as used herein includes any and all combinations of one or more of the associated listed items.

[0043] Please see Figure 1The cleaning and separation device includes a mounting column 21, a mounting frame 22, a positioning plate 23, a cleaning needle assembly 24, a gripper assembly 25, a cleaning station assembly 26, a magnetic separation assembly 27, a mixing assembly 28, and a translation drive assembly 29. The mounting frame 22 is mounted to the mounting column 21 via the positioning plate 23, and its position is adjustable. The cleaning needle assembly 24 and the gripper assembly 25 are slidably mounted on the mounting frame 22, facilitating the gripping of the reaction vessel 100 and the cleaning of the reactants inside the reaction vessel 100. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are fixed to the mounting frame 22 and located on the same straight line. This design allows the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 to be installed or disassembled together, facilitating equipment assembly. The cleaning station assembly 26, magnetic separation assembly 27, and mixing assembly 28 are located on the same straight line, so that when the cleaning needle assembly 24 and gripper assembly 25 move linearly along the mounting frame 22, any one of the cleaning station assembly 26, magnetic separation assembly 27, and mixing assembly 28 can be operated. The translation drive assembly 29 is mounted on the mounting frame 22 to drive the translation of the cleaning needle assembly 24 and gripper assembly 25.

[0044] Please continue reading. Figure 2 as well as Figure 3 The mounting post 21 includes an end 210, which is circular.

[0045] Mounting bracket 22 includes a vertical plate 220 and a horizontal plate 221, which are fixedly connected. The vertical plate 220 is used to mount the translation drive assembly 29, the cleaning needle assembly 24, and the gripper assembly 25, which are slidably mounted on the vertical plate 220. The cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28 are fixed to the horizontal plate 221. The horizontal plate 221 has an adjustment hole 2210 and a fixing hole 2211. The adjustment hole 2210 is circular, and its diameter is larger than the diameter of the end 210, allowing the mounting bracket 22 to be adjusted in position when mounted on the mounting column 21.

[0046] The positioning piece 23 is provided with a locking hole 231 and a positioning groove 230. The locking hole 231 cooperates with the end 210, and the position of the positioning groove 230 corresponds to the fixing hole 2211. The locking element is installed in the fixing hole 2211 and the positioning groove 230 to lock the positioning piece 23 to the horizontal plate 221. Specifically, the positioning groove 230 is U-shaped.

[0047] Please continue reading. Figure 4The cleaning needle assembly 24 includes a vertical drive 240, a rack 241, a base 242, a bearing 243, and a cleaning needle 244. The rack 241 is slidably mounted on a vertical plate 220, and the vertical drive 240 is slidably mounted on the rack 241. A gear is mounted on the output shaft of the vertical drive 240, meshing with the rack 241. The base 242 is connected to the vertical drive 240 and moves vertically with it. The bearing 243 is installed within the base 242, and the cleaning needle 244 is rotatably mounted on the base 241 via the bearing 243. The cleaning needle 244 includes an aspiration needle and a cleaning needle, which are parallel. Both the aspiration needle and the cleaning needle are connected to a tubing. Because the cleaning needle 244 is rotatably mounted on the base 241, when the tubing becomes coiled, the cleaning needle 244 rotates within the base 241, preventing the tubing from tangling.

[0048] The gripper assembly 25 is slidably mounted on the vertical plate 220. The gripper assembly 25 is used to grip and lower the reaction cup 100.

[0049] The cleaning station component 26 is fixed to the horizontal plate 221.

[0050] The magnetic separation assembly 27 includes a heating element 270, a separation base 271, and a magnet 272. The separation base 271 is fixed to the horizontal plate 221, and the heating element 270 is installed at the bottom of the separation base 271 to control its temperature. Specifically, the heating element 270 is a Peltier magnet. The magnet 272 is installed inside the separation base 271 and attracts the magnetic beads inside the reaction vessel 100 to facilitate the cleaning of the reactants. For more details, please refer to [link / reference needed]. Figure 6 There are four magnets 272 arranged in a cross shape, with any two magnets 272 on the same straight line having the same magnetism. The four magnets 272 form a space to accommodate the reaction cup. In this embodiment, there are two magnetic separation components 27, which are arranged side by side.

[0051] The mixing assembly 28 includes a mixing motor 280, a mixing base 281, and a mixing head 282. The mixing motor 280 is fixed to the horizontal plate 221, and the mixing base 281 is installed at the output end of the mixing motor 280. The mixing motor 280 drives the mixing base 281 to rotate around its axis. The mixing head 282 is installed on the mixing base 281, and the axis of the mixing head 282 forms an angle with the axis of the mixing base 281, making the reactants in the reaction cup 100 installed on the mixing head 282 more uniformly mixed. In this embodiment, there are two mixing assemblies 28, which are arranged side by side.

[0052] Please continue reading. Figure 1The translation drive assembly 29 includes a translation drive component 290, a rotating shaft 291, and a belt 292. The translation drive component 290 is fixed to the vertical plate 220. There are two rotating shafts 291, which are rotatably mounted on the vertical plate 220. The belt 292 is sleeved between the two rotating shafts 291. The translation drive component 290 is connected to one of the rotating shafts 291, and drives the rotating wheel to rotate, causing the belt 292 to rotate. The cleaning needle assembly 24 and the gripper assembly 25 are mounted on the belt 292 and are driven by the belt 292 to move between the cleaning station assembly 26, the magnetic separation assembly 27, and the mixing assembly 28. There are two translation drive assemblies 29. The cleaning needle assembly 24 and the gripper assembly 25 are respectively mounted on the two translation drive assemblies 29, so that the cleaning needle assembly 24 and the gripper assembly 25 are driven independently without interference.

[0053] When using the cleaning and separation device, the mounting bracket 22 is installed on the mounting column 21. After adjusting the position of the mounting bracket 22, the positioning piece 23 locks the mounting bracket 22 and the mounting column 21. The translation drive assembly 29 drives the gripper assembly 25 to translate to the incubation tray, grabs the reaction cup 100 on the incubation tray, and moves the reaction cup 100 to the magnetic separation assembly 27. The magnet 272 attracts the magnet 272 in the reaction cup 100, and the suction needle of the cleaning needle 244 assembly 24 draws liquid from the reaction cup 100. The liquid cleaning needle 244 component 24 adds buffer solution to the reaction cup 100, cleaning the reactants in the reaction cup 100. The gripper component 25 grips the reaction cup 100 from the magnetic separation component 27 and transfers it to the mixing component 28. The mixing motor 280 drives the mixing base 281 to rotate, mixing the reactants in the reaction cup 100. The gripper component 25 then grips the reaction cup 100 from the mixing component 28 and transfers it to the incubation tray, where the incubation tray incubates the reactants in the reaction cup 100. During the use of the cleaning and separation device, the rotation of the incubation tray is involved, which delivers the reaction cup 300. The incubation tray determines whether to deliver a cup based on a control signal. When delivery is required, it checks if there is a reaction cup 100 at the delivery position on the incubation tray. If there is, the gripper component 25 grips the cup.

[0054] This application also discloses a method for using the above-mentioned cleaning and separation device, including the following steps:

[0055] Install the mounting bracket 22 onto the mounting post 21. After adjusting the position of the mounting bracket 22, lock the mounting bracket 22 and the mounting post 21 together using the positioning piece 23.

[0056] The translation drive assembly 29 drives the gripper assembly 25 to translate to the incubation tray, grab the reaction cup 100 on the incubation tray and move the reaction cup 100 to the magnetic separation assembly 27;

[0057] Magnet 272 attracts magnet 272 in reaction cup 100, liquid suction needle of cleaning needle 244 assembly 24 draws liquid from reaction cup 100, and dispensing needle of cleaning needle 244 assembly 24 adds buffer solution to reaction cup 100 to achieve cleaning of reactants in reaction cup 100.

[0058] The gripper assembly 25 grips the reaction cup 100 on the magnetic separation assembly 27 and moves it to the mixing assembly 28. The mixing motor 280 drives the mixing base 281 to rotate, and the reactants in the reaction cup 100 are mixed.

[0059] The gripper assembly 25 grips the reaction cup 100 on the mixing assembly 28 and places it into the incubation tray, where the incubation tray incubates the reactants in the reaction cup 100.

[0060] In actual use, the incubation tray rotates, and the two magnetic separation components 27 and the two mixing components 28 work in an alternating manner. The two magnetic separation components 27 are the first magnetic separation component 273 and the second magnetic separation component 274, and the two mixing components 28 are the first mixing component 283 and the first mixing component 284. Odd-numbered and even-numbered cycles form a loop, which improves the analysis efficiency.

[0061] Please continue reading. Figure 7 When operating in odd-numbered cycles, the workflow is as follows:

[0062] Determine if the incubation tray has been filled with cups;

[0063] (1) When the incubation tray delivers a cup, it is determined whether the first magnetic separation component 273 has a cup. A variable is set in the software for each position of the two magnetic separation components 27 and the two mixing components 28. According to the process action, the variable is set to 1 when the cup is placed and 0 when the cup is removed.

[0064] (11) If a cup is present, the device is in an abnormal state and an alarm will be triggered.

[0065] (12) When there is no cup, the gripper assembly 25 picks up the cup from the incubation tray and places it into the first magnetic separation assembly 273, and then determines whether the second mixing assembly 284 has a cup.

[0066] (121) When there is a cup, the gripper assembly 25 grabs the cup on the second mixing assembly 284 and places it on the incubation tray, and then determines whether the second magnetic separation assembly 274 has a cup. (1211) When there is a cup, the gripper assembly 25 grabs the cup and places it on the second mixing assembly 284, and the gripper assembly 25 returns to the origin and remains stationary. (1212) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0067] (122) When there is no cup, the gripper assembly 25 returns to the origin and determines whether the second magnetic separation assembly 274 has a cup. (1221) When there is a cup, the gripper assembly 25 grabs the cup of the second magnetic separation assembly 274 and moves it to the second mixing assembly 284. The gripper assembly 25 returns to the origin and remains stationary. (1222) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0068] (2) When the incubation tray does not deliver the cup, determine whether the second mixing component 284 has a cup.

[0069] (21) When there is a cup, the gripper assembly 25 grips the cup to the incubation tray and determines whether the second magnetic separation assembly 274 has a cup;

[0070] (211) When there is a cup, the gripper assembly 25 grabs the cup and the second mixing assembly 284, and the gripper assembly 25 returns to the origin and stops; (211) When there is no cup, the gripper assembly 25 stops at the origin;

[0071] (22) When there is no cup, the gripper assembly 25 returns to the origin and determines whether the second magnetic separation assembly 274 has a cup;

[0072] (221) When there is a cup, the gripper assembly 25 grabs the cup to the second mixing assembly 284, and the gripper assembly 25 returns to the origin and stops; (222) When there is no cup, the gripper assembly 25 stops at the origin.

[0073] Please continue reading. Figure 8 When operating in even-numbered cycles, the workflow is as follows:

[0074] Determine if the incubation tray has been filled with cups;

[0075] (1) When the incubation tray delivers a cup, it is determined whether the second magnetic separation component 274 has a cup. A variable is set in the software for each position of the two magnetic separation components 27 and the two mixing components 28. According to the process action, the variable is set to 1 when the cup is placed and 0 when the cup is removed.

[0076] (11) If a cup is present, the device is in an abnormal state and an alarm will be triggered.

[0077] (12) When there is no cup, the gripper assembly 25 picks up the cup from the incubation tray and transfers it to the second magnetic separation assembly 274, and then determines whether the first mixing assembly 283 has a cup.

[0078] (121) When there is a cup, the gripper assembly 25 grabs the cup on the first mixing assembly 283 and places it on the incubation tray, and then determines whether the first magnetic separation assembly 273 has a cup. (1211) When there is a cup, the gripper assembly 25 grabs the cup and places it on the first mixing assembly 283, and the gripper assembly 25 returns to the origin and remains stationary. (1212) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0079] (122) When there is no cup, the gripper assembly 25 returns to the origin and determines whether the first magnetic separation assembly 273 has a cup. (1221) When there is a cup, the gripper assembly 25 grabs the cup of the first magnetic separation assembly 273 and moves it to the first mixing assembly 283. The gripper assembly 25 returns to the origin and remains stationary. (1222) When there is no cup, the gripper assembly 25 remains stationary at the origin.

[0080] (2) When the incubation tray does not deliver the cup, determine whether the first mixing component 283 has a cup.

[0081] (21) When there is a cup, the gripper assembly 25 grips the cup onto the incubation tray and determines whether the first magnetic separation assembly 273 has a cup;

[0082] (211) When there is a cup, the gripper assembly 25 grabs the cup and the first mixing assembly 283, and the gripper assembly 25 returns to the origin and stops; (211) When there is no cup, the gripper assembly 25 stops at the origin;

[0083] (22) When there is no cup, the gripper assembly 25 returns to the origin and determines whether the first magnetic separation assembly 273 has a cup;

[0084] (221) When there is a cup, the gripper assembly 25 grabs the cup and the first mixing assembly 283, and the gripper assembly 25 returns to the origin and stops; (222) When there is no cup, the gripper assembly 25 stops at the origin.

[0085] Compared with the prior art, the mounting column 21 of the cleaning and separation device of the present invention includes an end 210, and the horizontal plate 221 is provided with an adjustment hole 2210. The diameter of the adjustment hole 2210 is larger than the diameter of the end 210. The end 210 is located in the adjustment hole 2210 and its position can be adjusted so that the position of the mounting frame 22 relative to the mounting column 21 can be adjusted. The positioning piece 23 fixes the mounting column 21 and the mounting frame 22. The cleaning station assembly 26, the magnetic separation assembly 27 and the mixing assembly 28 are fixed to the horizontal plate 221 and are located on the same straight line, so that the cleaning station assembly 26, the magnetic separation assembly 27 and the mixing assembly 28 can be installed and disassembled as a whole. The cleaning station assembly 26, the magnetic separation assembly 27 and the mixing assembly 28 are located on a straight line, which facilitates the movement of the gripper assembly 25. The cleaning needle 244 is rotatably mounted on the base 242 through the bearing 243. The cleaning needle 244 is connected to the pipeline. When the pipeline is looped, the cleaning needle 244 rotates to prevent the pipeline from looping. The four magnets 272 are arranged in a cross shape, and the two magnets 272 located on the same straight line have the same magnetism, which makes the magnets 272 adsorb the magnetic beads in the reaction cup 100 better; the axis of the mixing head 282 forms an angle with the axis of the mixing base 281, so that when the mixing base 281 rotates, the reactants in the reaction cup 100 in the mixing head 282 are mixed more evenly.

[0086] The above embodiments merely illustrate several implementation methods of the present invention, and their descriptions are relatively specific and detailed, but they should not be construed as limiting the scope of the invention patent. It should be noted that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present invention. These are all equivalent modifications and improvements made to the above embodiments based on the essential technology of the present invention, and all of these fall within the protection scope of the present invention.

Claims

1. A cleaning and separation device, including a mounting frame, characterized in that: The mounting frame includes a vertical plate and a horizontal plate fixed to the vertical plate. The cleaning and separation device also includes a mounting column, a positioning plate, a cleaning needle assembly, a gripper assembly, a cleaning station assembly, a magnetic separation assembly, a mixing assembly, and a translation drive assembly. The translation drive assembly includes a translation drive component, a rotating shaft, and a belt. The cleaning needle assembly and the gripper assembly are connected to the translation drive component. The cleaning station assembly, the magnetic separation assembly, and the mixing assembly are fixed to the horizontal plate and located on the same straight line. The magnetic separation assembly is located between the cleaning station assembly and the mixing assembly. The mounting column includes an end. The horizontal plate is provided with an adjustment hole. The diameter of the adjustment hole is larger than the diameter of the end. The end is located in the adjustment hole and can be adjusted so that the position of the mounting frame relative to the mounting column can be adjusted. The positioning plate fixes the mounting column and the mounting frame. The horizontal plate is also provided with a fixing hole, and the positioning piece is provided with a locking hole and a positioning groove. The locking hole is fixed to the end of the mounting column, and the position of the positioning groove corresponds to the fixing hole. The screw passes through the fixing hole, extends into the positioning groove, and cooperates with the nut to fix the mounting column and the mounting bracket. The magnetic separation assembly includes a separation base and a magnet. The separation base is fixed to the horizontal plate, and the magnet is installed inside the separation base. The number of magnets is four, and the four magnets are arranged in a cross shape. The two magnets located on the same straight line have the same magnetism, and the space between the four magnets forms a space to contain the reaction cup.

2. The cleaning and separation device according to claim 1, characterized in that: The translation drive is fixed to the vertical plate. There are multiple rotating shafts, which are rotatably mounted on the vertical plate. The belt is sleeved on the rotating shaft. The output end of the translation drive is fixedly connected to one of the rotating shafts. The cleaning needle assembly and the gripper assembly are connected to the belt.

3. The cleaning and separation device according to claim 1, characterized in that: The cleaning needle assembly includes a base, a bearing, and a cleaning needle. The base is mounted on the vertical plate, the bearing is installed inside the base, and the cleaning needle is rotatably mounted on the base via the bearing.

4. The cleaning and separation device according to claim 3, characterized in that: The cleaning needle assembly also includes a vertical drive and a rack. A gear is installed at the output end of the vertical drive, and the gear meshes with the rack to drive the base to move in the vertical direction.

5. The cleaning and separation device according to claim 1, characterized in that: The mixing assembly includes a mixing motor, a mixing base, and a mixing head. The mixing motor is fixed to the horizontal plate, the mixing base is installed at the output end of the mixing motor, the mixing motor drives the mixing base to rotate, and the mixing head is installed on the mixing base. The mixing head is used to install the reaction cup.

6. The cleaning and separation device according to claim 5, characterized in that: The axis of the mixing head forms an angle with the axis of the mixing base.

7. The method of using the cleaning and separation device as described in any one of claims 1-6, characterized in that, Includes the following steps: Install the mounting bracket onto the mounting post, and after adjusting the position of the mounting bracket, lock the mounting bracket to the mounting post using the positioning plate; The translation drive component drives the gripper component to translate to the incubation tray, grabs the reaction cup on the incubation tray, and moves the reaction cup to the magnetic separation component; A magnet attracts the magnetic beads in the reaction cup, the liquid suction needle of the cleaning needle assembly draws up the liquid in the reaction cup, and the dispensing needle of the cleaning needle assembly adds buffer solution to the reaction cup, thereby cleaning the reactants in the reaction cup. The gripper assembly grabs the reaction cup on the magnetic separation assembly and moves it to the mixing assembly. The mixing motor drives the mixing base to rotate, and the reactants in the reaction cup are mixed. The gripper assembly picks up the reaction cup from the mixing assembly and places it into the incubation tray, where the incubation tray incubates the reactants in the reaction cup. Once the cleaning needle assembly completes one cycle, it moves to the cleaning station for cleaning.

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