Small animal in-vitro lung perfusion device

Abstract

The invention particularly relates to a small animal in-vitro lung perfusion device which comprises a box body, and the space in the box body is divided into an operation bin and a perfusion bin through a partition plate; the perfusion tube and the moving plate are driven by the driving mechanism, so that accurate perfusion of perfusion liquid is ensured; a rotating plate and a partition plate of the device are combined, the relative sealing performance of an operation bin and a perfusion bin is guaranteed, external pollution is reduced, clamping of the lung trachea is controlled through a clamping mechanism and a lifting electric cylinder, stable connection of the trachea and a branch pipe is guaranteed, liquid leakage in the perfusion process is avoided, the trachea and the branch pipe are perfectly attached through a conical and ball head structure of the branch pipe, and the perfusion efficiency is improved. The perfusion effect is improved; the position of the storage box is adjusted through the lifting mechanism, the taking and placing operation of the lung tissue is simplified, friction of a rotating plate is reduced through a roller structure, and the durability and stability of the device are improved; the small animal in-vitro lung perfusion device has the advantages of high efficiency, automation, accurate control and the like.

Description

Technical Field

[0001] The present invention relates to the technical field of animal experiment equipment, and particularly to a small animal ex vivo lung perfusion device. Background Art

[0002] With the continuous development of biomedical research, especially in the fields of drug R & D and toxicology research, animal experiments have gradually become an important means to evaluate drug safety, study disease mechanisms, and test treatment effects. As a common experimental method, small animal ex vivo lung perfusion experiments have been widely used in lung drug delivery, lung pathology research, and experiments related to lung perfusion. However, in traditional lung perfusion experiments, there are multiple operation difficulties, such as: suspension and fixation of lung tissue, connection of trachea and bronchus, and delivery of perfusion fluid, etc. The existence of these problems makes the experimental process complex and difficult to achieve precise control, thus affecting the repeatability of the experiment and the accuracy of the data.

[0003] Existing ex vivo lung perfusion devices usually rely on manual operations to complete tasks such as lung suspension and trachea clamping, which not only increases the operation difficulty, but also may cause lung damage or instability of experimental data. In addition, it is currently an urgent technical problem to quickly remove the trachea of the perfused lung from the perfusion mechanism and how to reduce the external overflow of the internal fixing fluid after removal.

[0004] Therefore, we provide a small animal ex vivo lung perfusion device that can achieve precise suspension of lung tissue, stable connection of trachea and bronchus, and solve the problem of cumbersome manual operation in the prior art through an automated operation and precise control system. Summary of the Invention

[0005] The purpose of the present invention is to overcome the deficiencies in the prior art and provide a small animal ex vivo lung perfusion device.

[0006] The purpose of the present invention is achieved as follows: A small animal ex vivo lung perfusion device includes a box body. A partition is arranged inside the box body, and the partition divides the space inside the box body into an operation chamber and a perfusion chamber. The operation chamber is located on the left side of the perfusion chamber. A plurality of perfusion tubes that can move left and right are arranged inside the box body. A plurality of branch tubes are arranged at the right part of each perfusion tube, and a clamping mechanism is arranged at the branch tube. The clamping mechanism is used to clamp the lung. A rotating plate is arranged above the partition, and the upper end of the rotating plate is rotatably connected to the inside of the box body. A storage box that can move up and down is arranged at the bottom of the perfusion chamber. Storage cylinders corresponding to the branch tubes are arranged inside the storage box, and each storage cylinder is located directly below the branch tube. A strip-shaped opening is arranged on the right side of the box body, and the storage box can be moved out of the box body through the strip-shaped opening.

[0007] Furthermore, the clamping mechanism includes a plurality of fixed clamps that can move up and down. The fixed clamps can clamp the trachea of the lung. The lower end of the branch pipe is a conical structure. A ball head structure is provided on the branch pipe above the conical structure. The conical structure facilitates guiding the trachea of the lung onto the branch pipe, and the ball head structure can make the trachea of the lung fit tightly with the branch pipe for sealing.

[0008] Furthermore, the clamping mechanism further includes a rotating disk. The branch pipe passes through the center of the rotating disk, and the branch pipe is rotatably connected to the rotating disk. The rotating disk is provided with lifting electric cylinders corresponding to the number of fixed clamps. The lifting electric cylinders can move radially along the rotating disk. A connecting plate is provided at the lower end of the lifting electric cylinder, and the lower end of the connecting plate is connected to the fixed clamp.

[0009] Furthermore, a lifting cylinder is provided at the lower end of the connecting plate. A connecting rod that can move up and down is provided in the lifting cylinder, and the lower end of the connecting rod is connected to the fixed clamp.

[0010] Furthermore, the fixed clamp includes a clamp body. The left side of the clamp body extends downward. A movable clamp that can move left and right is provided at the lower part of the clamp body. A guide rod is provided at the lower part of the movable clamp body. The guide rod passes through the movable clamp. A spring is provided on the guide rod on the right side of the movable clamp. The left and right ends of the spring are respectively connected to the movable clamp and the clamp body. A moving groove is provided on the clamp body above the movable clamp. A limiting plate that can move left and right is provided in the moving groove. The limiting plate is located on the left side of the movable clamp. A telescopic electric cylinder is provided in the clamp body, and the telescopic electric cylinder drives the limiting plate to move left and right.

[0011] Furthermore, a fixed disk is rotatably provided above the rotating disk. The branch pipe passes through the center of the fixed disk, and the fixed disk is fixedly connected to the branch pipe. An inclined groove and an arc groove are provided on the rotating disk above each lifting electric cylinder. The arc groove is communicated with the inclined groove. The central axis of the arc groove is coaxial with the central axis of the branch pipe. The inclined groove is located outside the arc groove. A cylindrical rod is connected to the upper end of the lifting electric cylinder. The cylindrical rod is located in the inclined groove, and the cylindrical rod can move in the inclined groove and the arc groove; a radial groove is provided on the fixed disk above each arc groove. The radial groove radiates outward along the central axis of the fixed disk. A guiding block is provided at the upper end of the cylindrical rod. The guiding block is located in the radial groove, and the guiding block can move in the radial groove; a guiding plate is fixedly provided at the upper part inside the lifting electric cylinder. A wire groove is provided on the guiding plate. A fixed rod is provided on the rotating disk outside each arc groove. A pulling wire is connected to the fixed rod. The pulling wire can move up and down through the wire groove. The lower end of the pulling wire is connected to the upper end of the connecting rod. The upper end of the connecting rod is a conical structure, and the inner side of the lower end of the lifting cylinder is an inward concave conical structure.

[0012] Furthermore, a driving motor is provided on the connecting frame on one side of each rotating disk. The driving motor drives the rotating plate to rotate. A driving gear is provided at the output end of the driving motor. A driven gear is provided on the outer side surface of the rotating disk. The driven gear meshes with the driving gear.

[0013] Furthermore, on the boxes on the front and rear sides of the operation chamber, there are openable and closable first opening doors, and the first opening doors are made of transparent materials; on the front and rear sides of the perfusion chamber, there are second opening doors, and the second opening doors are made of transparent materials. The provision of the second opening doors facilitates regular cleaning of the internal space, etc., and the transparent materials facilitate observation of the interior.

[0014] When this application is in use, the controller controls the driving mechanism to drive the moving plate to move leftward. When the moving plate moves leftward, it drives the perfusion tube to move leftward. When the perfusion tube moves leftward, the connecting frame on the perfusion tube moves leftward. When the roller on the connecting frame comes into contact and fits with the rotating plate, as the roller moves leftward driven by the connecting frame, the roller squeezes and fits with the rotating plate, driving the rotating plate to rotate. The perfusion tube continues to move leftward, and the rotating plate opens to allow the connecting frame to move leftward into the operation chamber.

[0015] Open the first opening door in the operation chamber, and the hanging operation of the lung tissue can be carried out in the operation chamber (that is, the lung tissue is clamped and hung by the clamping mechanism); that is, the experimenter takes out the animal lung tissue and hangs it on the branch pipe; then close the first opening door, and then the controller controls the driving mechanism to drive the moving plate to move rightward, so as to push the perfusion tube rightward. When the roller disengages from the limit of the rotating plate, the rotating plate rotates downward under the action of its own gravity, and then the lower end of the rotating plate fits with the upper end of the partition; the perfusion tube continues to move rightward, and the strip plate seals the strip groove, thereby realizing the relative sealing operation of the operation chamber and the perfusion chamber, ensuring the relatively sealed space in the perfusion chamber, and reducing the pollution of the lung by bacteria and the like from the outside.

[0016] The fixing liquid is instilled into the lung tissue through the perfusion tube. When instilling the fixing liquid, appropriate parameters such as pressure, temperature, and flow rate can be selected according to needs. The specific liquid supply method is not technically limited, as long as the above functions can be achieved. The outer end of the perfusion tube can be connected to a flexible tube to facilitate the left and right movement of the perfusion tube, and the specific details are not technically limited. After the instillation of the lung tissue is completed. After the instillation is completed, the lifting mechanism drives the storage box to move upward, so that the lung tissue is located in the corresponding storage cylinder below (the fixing liquid corresponding to the perfusion tube is placed in the storage cylinder). The clamping action on the lung tissue is released by the clamping mechanism, and the lung tissue falls into the storage cylinder below under the action of its own gravity; then the lifting mechanism resets the storage box, and the storage box can be pulled out from the strip opening to complete the lung perfusion operation.

[0017] According to needs, the lung tissue after instillation can be moved into the operation chamber and dissected, or the second sealing door can be opened to dissect the lung tissue in the instillation chamber.

[0018] When connecting the pulmonary trachea and the bronchus (clamping operation), it is connected by a clamping mechanism. By controlling the driving mechanism, the rotating disk is driven to rotate. When the rotating disk rotates, the cylindrical rod above the lifting electric cylinder moves in the inclined groove. The inclined groove squeezes the cylindrical rod, thereby driving the guiding block to move in the radial groove, realizing the synchronous movement of multiple lifting electric cylinders towards the center of the rotating disk. At this time, the pulling wire is slack, and the connecting rod moves downward under the action of its own gravity. The rotating disk continues to rotate, and the cylindrical rod moves in the arc groove. At this time, the lifting electric cylinder no longer moves towards the center, the pulling rope continues to be slack, and the connecting rod continues to move downward. At this time, the fixed clamp moves downward, and the lower end of the pulling rope disengages from the lifting cylinder.

[0019] At this time (since the rear end of the fixed clamp is connected by a pulling rope, it can be conveniently held by hand), it is more convenient to hold the fixed clamp by hand to clamp the connection between the pulmonary trachea and the bronchus. Multiple fixed clamps are evenly clamped on the connection of the pulmonary trachea. That is, when clamping, manually pull the movable clamp outward, place the side wall of the pulmonary trachea between the movable clamp and the clamp body, and release the movable clamp. The movable clamp moves inward under the action of the spring force to clamp and fix the side wall of the pulmonary trachea. At this time, the controller is used to control the rotating disk to rotate in the reverse direction. When rotating, first the pulling rope is tightened (when the cylindrical rod moves in the arc groove), the connecting rod moves upward. The upper end of the connecting rod is a conical structure, and the inner side of the lower end of the lifting cylinder is an inward concave conical structure, which can conveniently place the connecting rod in the lifting cylinder under the drive of the pulling rope. The lower part of the connecting rod and the inside of the lifting cylinder are in clearance fit to avoid the shaking of the connecting rod and at the same time lift the pulmonary trachea.

[0020] The rotating disk continues to rotate, the cylindrical rod moves in the inclined groove, the pulling rope continues to contract, driving the fixed clamp to continue to move upward; at the same time, the lifting electric cylinder moves outward, thereby driving the fixed clamp to move outward. Multiple fixed clamps cooperate to stretch and expand the connection port of the pulmonary trachea to form a certain opening, which is convenient for placing the bronchus into the opening. Then, when the controller controls the lifting electric cylinder to move upward, it drives the pulmonary trachea to move upward. At this time, the conical structure at the lower end of the bronchus can conveniently enter the opening of the pulmonary trachea that has been expanded. The lifting electric cylinder continues to move upward. Since the lower part of the bronchus is a conical structure, the opening can be quickly and gradually fitted to the bronchus. When the opening of the pulmonary trachea is relatively large, the lifting electric cylinder continues to move upward, so that the opening enters the ball head mechanism, and the ball head structure is used to squeeze and seal the opening to prevent liquid from overflowing during the subsequent perfusion process.

[0021] After the perfusion is completed, the controller controls the telescopic electric cylinder to contract, which drives the limit plate to move. The limit plate drives the movable clamp to move outward and release the clamping effect on the lung trachea. The lung trachea moves downward under the action of its own gravity and disengages from the branch pipe or the ball head structure, and then enters the storage cylinder below. The fixing solution placed in the storage barrel can effectively preserve the lung tissue after perfusion, preventing a large amount of the fixing solution from overflowing; and control the lifting mechanism to move downward to reset the storage box, then the storage box can be pulled outwards, facilitating the subsequent processing of the lung tissue in the storage cylinder. Resetting all the structures enables the next round of perfusion operation.

[0022] Through innovative sealing structures, automated control, precise perfusion management, stable clamping systems, and convenient experimental process designs, this application significantly improves the reliability and operability of experiments. The high-efficiency automation of the device not only reduces human errors, improves the repeatability of experiments, but also can adapt to different experimental requirements, giving it broad application prospects in fields such as physiology, pathology, and drug research.

[0023] Beneficial effects:

[0024] 1. Improve the sealing performance to ensure a clean experimental environment:

[0025] With structural designs such as partition plates, rotating plates, and strip grooves, the operation chamber and the perfusion chamber can be effectively isolated to achieve a relatively sealed environment. This design can effectively prevent external pollutants (such as dust and bacteria in the air) from entering the experimental system, reducing the risk of the lung tissue being interfered by the outside world, thus ensuring the accuracy and repeatability of experimental results. In addition, the ball head sealing design of the branch pipe further enhances the liquid sealing effect, preventing the fixing solution from overflowing during perfusion and improving the success rate of the experiment.

[0026] 2. Achieve high-efficiency automation and reduce manual intervention:

[0027] Integrating a driving mechanism, a lifting mechanism, and a clamping mechanism enables the entire process of clamping, perfusion, and releasing of the lung tissue to be automated. Compared with traditional manual operations, this device can significantly reduce the influence of human factors, improve the standardization of experiments, while reducing the work intensity of experimental personnel and the possibility of misoperations. This automated design is particularly suitable for high-throughput experimental requirements and helps to improve research efficiency.

[0028] 3. Precisely control perfusion parameters to improve experimental controllability;

[0029] The device can precisely control the perfusion process by adjusting parameters such as the pressure, flow rate, and temperature of the perfusion tube. This feature enables experimenters to flexibly adjust the perfusion conditions according to specific experimental requirements, simulate different physiological or pathological environments, and improve the controllability of the experiment. In addition, the conical introduction structure of the branch tube allows the trachea of the lung to be smoothly inserted and closely attached to the branch tube, ensuring that the fixing solution is evenly perfused into the lung tissue and improving the stability and consistency of the experiment.

[0030] 4. Innovative clamping mechanism to improve the stability of tissue fixation;

[0031] A multifunctional clamping mechanism is adopted, including components such as fixed clamps, spring limit devices, lifting electric cylinders, and rotating disks, enabling the trachea of the lung to be stably clamped and reliably connected to the branch tube. The lifting and radial adjustment functions of the clamping mechanism can accurately position the lung tissue before the experiment, ensure a seamless connection between the trachea and the branch tube, and prevent liquid leakage or tissue detachment. This improvement enhances the success rate of the perfusion experiment and reduces tissue damage during the experiment.

[0032] 5. Convenient design for taking and placing lung tissue to improve operation convenience:

[0033] The bottom of the device is equipped with a storage box and a storage cylinder that can move up and down, enabling the automatic release of the lung tissue after the experiment and preservation with the fixing solution. Experimenters do not need to manually remove the lung tissue, avoiding the risk of contamination during the operation. In addition, the strip-shaped opening design of the storage box makes it convenient to take out the lung tissue after the experiment, simplifies the subsequent experimental process, and improves the convenience and safety of the experimental operation.

[0034] 6. Adopting a roller structure to reduce friction and improve the durability of the equipment:

[0035] The rotating plate adopts a roller-assisted structure, reducing the friction between components during movement, thereby reducing the mechanical wear of the equipment and improving the durability of the device. This design optimizes the long-term use performance of the equipment, reduces the maintenance cost caused by component wear, and enables the laboratory to conduct long-term research more stably.

[0036] 7. Visual experimental environment for easy monitoring and maintenance:

[0037] Transparent sealed doors and observation windows are provided on both the front and back sides of the box, allowing experimenters to monitor the experimental status in real time without frequently opening the equipment for inspection, thereby reducing the risk of contamination during the experiment. In addition, the transparent structure makes the cleaning and maintenance of the equipment more convenient. Experimenters can timely discover and clean the residues after the experiment to ensure the cleanliness of the next experimental environment.

[0038] 8. More integrated structure and comprehensive functions:

[0039] By rotating the rotating disk, not only can the clamping mechanism be lowered, but also the clamping mechanism can be driven to move inward or outward within the box. The lowering of the clamping structure in conjunction with the connection of the pulling rope can make it easier for the fixed clamp to hold, and at the same time, the rising of the clamping mechanism can keep the fixed clamp within the lifting cylinder to prevent it from shaking. The outward movement of the clamping mechanism can expand the lung trachea outward for convenient connection with the branch pipe, and the rising of the clamping mechanism can achieve a tight connection between the lung trachea and the branch pipe or the ball head structure. Description of the Drawings

[0040] Figure 1 It is a schematic structural diagram of the invention.

[0041] Figure 2 It is a schematic right-side structural diagram of the invention.

[0042] Figure 3 It is a partial cross-sectional view of the box body structure of the invention.

[0043] Figure 4 It is a schematic partial structure diagram of the invention.

[0044] Figure 5 It is a schematic structural diagram of the invention after the rotating plate rotates.

[0045] Figure 6 It is a schematic structural diagram of the roller and connecting frame of the invention.

[0046] Figure 7 It is a schematic structural diagram of the clamping mechanism of the invention.

[0047] Figure 8 It is a schematic partial structure diagram of the clamping mechanism of the invention.

[0048] Figure 9 It is a bottom view of the clamping mechanism of the invention.

[0049] Figure 10 It is a schematic partial structure diagram of the clamping mechanism of the invention.

[0050] Figure 11 It is a schematic structural diagram of the pulling wire and fixed rod of the invention.

[0051] Figure 12 It is a partial cross-sectional view of the clip body of the invention.

[0052] Figure 13 It is a schematic structural diagram of the arc groove and inclined groove of the invention.

[0053] Description of the Reference Numerals:

[0054] 1. Box body, 2. Operation chamber, 3. Perfusion chamber, 4. Second opening and closing door, 5. Partition board, 6. First opening and closing door, 7. Driving mechanism, 8. Moving plate, 9. Perfusion tube, 10. Storage box, 11. Storage cylinder, 12. Rotating plate, 13. Strip-shaped opening, 14. Limiting block, 15. Roller, 16. Connecting frame, 17. Strip-shaped plate, 18. Lifting mechanism, 19. Lifting cylinder, 20. Branch pipe, 21. Ball head structure, 22. Connecting rod, 23. Lifting electric cylinder, 24. Rotating disk, 25. Driven gear, 26. Fixed disk, 27. Driving motor, 28. Driving gear, 29. Guide block, 30. Clamping body, 31. Pulling wire, 32. Connecting plate, 33. Fixed rod, 34. Arc-shaped groove, 35. Inclined groove, 36. Guide plate, 37. Telescopic electric cylinder, 38. Spring, 39. Moving clamp, 40. Limiting plate. Detailed implementation manner

[0055] Example 1, as Figures 1-13 shown, the object of the present invention is achieved as follows: a small animal ex vivo lung perfusion device includes a box body 1. A partition board 5 is arranged inside the box body 1. The partition board 5 divides the space inside the box body 1 into an operation chamber 2 and a perfusion chamber 3. The operation chamber 2 is located on the left side of the perfusion chamber 3. A plurality of perfusion tubes 9 that can move left and right are arranged inside the box body 1. A plurality of branch pipes 20 are arranged at the right part of each perfusion tube 9. A clamping mechanism is arranged at the branch pipe 20 for clamping the lung. A rotating plate 12 is arranged above the partition board 5. The upper end of the rotating plate 12 is rotatably connected to the inside of the box body 1; a storage box 10 that can move up and down is arranged at the bottom of the perfusion chamber 3. Storage cylinders 11 corresponding to the branch pipes 20 are arranged inside the storage box 10. Each storage cylinder 11 is located directly below the branch pipe 20. A strip-shaped opening 13 is arranged on the right side of the box body 1. The storage box 10 can be moved out of the box body 1 through the strip-shaped opening 13.

[0056] The lower end of the rotating plate 12 is mutually attached to the upper end of the partition board 5. The lower end of the rotating plate 12 is an inclined surface structure that slopes downward to the left. The upper end surface of the partition board 5 and the lower end surface of the rotating plate 12 are mutually fitted; this can ensure the rotation of the rotating plate 12, and at the same time can increase the contact area between the partition board 5 and the rotating plate 12, increasing its sealing effect.

[0057] Strip-shaped grooves corresponding to the perfusion tubes 9 are opened on the rotating plate 12. The perfusion tubes 9 can move left and right through the strip-shaped grooves. A strip-shaped plate 17 is fixedly arranged at the left part of each perfusion tube 9. The strip-shaped plate 17 covers the strip-shaped grooves; realizing the relative sealing effect of the operation chamber 2 and the perfusion chamber 3.

[0058] A limiting block 14 is arranged at the upper left part inside the operation chamber 2. Each perfusion tube 9 can move left and right through the limiting block 14; the limiting block 14 has a certain thickness, which can ensure the horizontality of the perfusion tube 9 when it moves left and right.

[0059] A driving mechanism 7 is provided at the upper part of the box body 1. A moving plate 8 is provided at the front end of the driving mechanism 7. The driving mechanism 7 drives the moving plate 8 to move left and right. Each perfusion tube 9 is fixedly connected to the moving plate 8. The driving mechanism 7 is a prior art that can drive the moving plate 8 to move left and right, and no technical details will be described.

[0060] A connecting frame 16 is provided at the right part of the perfusion tube 9. A plurality of rotatable rollers 15 are evenly distributed on the upper surface of the connecting frame 16. The rollers 15 can squeeze the rotating plate 12 and reduce the friction between the two during relative movement. The lower surface of the rotating plate 12 is a slope structure. When the rollers 15 move to the right, they can well lift the rotating plate 12 to avoid movement interference.

[0061] A lifting mechanism 18 is provided at the bottom of the perfusion chamber 3. The lifting mechanism 18 drives the storage box 10 to move up and down. The clamping mechanism includes a plurality of fixed clamps that can move up and down. The fixed clamps can clamp the lung trachea. The lower end of the branch pipe 20 is a conical structure. A ball head structure 21 is provided on the branch pipe 20 above the conical structure. The conical structure facilitates guiding the lung trachea onto the branch pipe 20, and the ball head structure 21 can make the lung trachea fit tightly with the branch pipe 20 for sealing.

[0062] The clamping mechanism further includes a rotating disk 24. The branch pipe 20 passes through the center of the rotating disk 24, and the branch pipe 20 is rotatably connected to the rotating disk 24. The rotating disk 24 is provided with lifting electric cylinders 23 corresponding to the number of fixed clamps. The lifting electric cylinders 23 can move radially along the rotating disk 24. A connecting plate 32 is provided at the lower end of the lifting electric cylinder 23. The lower end of the connecting plate 32 is connected to the fixed clamp. A lifting cylinder 19 is provided at the lower end of the connecting plate 32. A connecting rod 22 that can move up and down is provided in the lifting cylinder 19. The lower end of the connecting rod 22 is connected to the fixed clamp.

[0063] The fixed clamp includes a clamp body 30. The left side of the clamp body 30 extends downward. A moving clamp 39 is provided at the lower part of the clamp body 30 and can move left and right. A guide rod is provided at the lower part of the clamp body 30. The guide rod passes through the moving clamp 39. A spring 38 is provided on the guide rod on the right side of the moving clamp 39. The left and right ends of the spring 38 are respectively connected to the moving clamp 39 and the clamp body 30. A moving groove is provided on the clamp body 30 above the moving clamp 39. A limiting plate 40 that can move left and right is provided in the moving groove. The limiting plate 40 is located on the left side of the moving clamp 39. A telescopic electric cylinder 37 is provided in the clamp body 30. The telescopic electric cylinder 37 drives the limiting plate 40 to move left and right.

[0064] Above the rotating disc 24, a fixed disc 26 is rotatably arranged. The branch pipe 20 penetrates through the central position of the fixed disc 26, and the fixed disc 26 is fixedly connected to the branch pipe 20. On the rotating disc 24 above each lifting electric cylinder 23, an inclined groove 35 and an arc groove 34 are formed. The arc groove 34 communicates with the inclined groove 35. The central axis of the arc groove 34 is coaxial with the central axis of the branch pipe 20. The inclined groove 35 is located outside the arc groove 34. The upper end of the lifting electric cylinder 23 is connected with a cylindrical rod, and the cylindrical rod is located in the inclined groove 35. The cylindrical rod can move in the inclined groove 35 and the arc groove 34. On the fixed disc 26 above each arc groove 34, a radial groove is formed, and the radial groove radiates outward along the central axis of the fixed disc 26. The upper end of the cylindrical rod is provided with a guiding block 29, and the guiding block 29 is located in the radial groove. The guiding block 29 can move in the radial groove. On the upper part inside the lifting electric cylinder 23, a guiding plate 36 is fixedly arranged, and a wire groove is formed on the guiding plate 36. On the rotating disc 24 outside each arc groove 34, a fixed rod 33 is arranged, and a pulling wire 31 is connected to the fixed rod 33. The pulling wire 31 can move up and down through the wire groove. The lower end of the pulling wire 31 is connected to the upper end of the connecting rod 22. The upper end of the connecting rod 22 is of a conical structure, and the inner side of the lower end of the lifting cylinder 19 is of a concave conical structure.

[0065] On the connecting frame 16 on one side of each rotating disc 24, a driving motor 27 is arranged. The driving motor 27 drives the rotating plate 12 to rotate. The output end of the driving motor 27 is provided with a driving gear 28, and the outer side surface of the rotating disc 24 is provided with a driven gear 25. The driven gear 25 meshes with the driving gear 28.

[0066] On the front and rear sides of the box body 1 of the operation chamber 2, openable first opening and closing doors 6 are arranged. The first opening and closing doors 6 are made of transparent materials. On the front and rear sides of the perfusion chamber 3, second opening and closing doors 4 are arranged. The second opening and closing doors 4 are made of transparent materials. Arranging the second opening and closing doors 4 can facilitate regular cleaning of the internal space, etc. The transparent materials can facilitate observation of the inside.

[0067] When the present application is used, the controller controls the driving mechanism 7 to drive the moving plate 8 to move leftward. When the moving plate 8 moves leftward, it drives the perfusion pipe 9 to move leftward. When the perfusion pipe 9 moves leftward, the connecting frame 16 on the perfusion pipe 9 moves leftward. When the roller 15 on the connecting frame 16 contacts and fits with the rotating plate 12, when the roller 15 moves leftward driven by the connecting frame 16, the roller 15 squeezes and fits with the rotating plate 12, driving the rotating plate 12 to rotate. The perfusion pipe 9 continues to move leftward, and the rotating plate 12 opens to allow the connecting frame 16 to move leftward into the operation chamber 2.

[0068] By opening the opening and closing door 6 in the operation chamber 2, the lung tissue suspension operation can be performed in the operation chamber 2 (i.e., the lung tissue is clamped and suspended by the clamping mechanism); that is, the experimenter takes out the animal lung tissue and suspends it on the branch pipe 20; then the opening and closing door 6 is closed, and the controller controls the driving mechanism 7 to drive the moving plate 8 to move to the right, so as to push the perfusion tube 9 to the right. When the roller 15 is out of the limit of the rotating plate 12, the rotating plate 12 rotates downward under the action of its own gravity, and then the lower end of the rotating plate 12 is fitted with the upper end of the partition 5; the perfusion tube 9 continues to move to the right, and the strip plate 17 blocks the strip groove, thereby realizing the relative sealing operation of the operation chamber 2 and the perfusion chamber 3, ensuring the relatively sealed space in the perfusion chamber 3, and reducing the contamination of the lungs by external bacteria.

[0069] The fixative is infused into the lung tissue through the perfusion tube 9. When the fixative is infused, appropriate parameters such as pressure, temperature, flow rate, etc. can be selected as needed. The specific liquid supply method is not technically limited, and the above functions can be achieved. The outer end of the perfusion tube 9 can be connected to a flexible tube to facilitate the left and right movement of the perfusion tube 9. There is no specific technical limitation. The perfusion of the lung tissue is completed. After the infusion is completed, the storage box 10 is driven to move upward by the lifting mechanism 18, so that the lung tissue is located in the corresponding storage tube 11 below (the storage tube 11 is filled with the fixative corresponding to the perfusion tube 9), and the clamping effect on the lung tissue is released by the clamping mechanism. The lung tissue falls into the storage tube 11 below under the effect of its own gravity; the storage box 10 is reset by the lifting mechanism 18, and the storage box 10 is pulled out from the strip opening 13 to complete the lung perfusion operation.

[0070] According to needs, the lung tissue after infusion can be moved to the operation chamber 2 and removed, or the sealed door 2 can be opened to remove the lung tissue in the infusion chamber.

[0071] When the lung trachea and the branch tube 20 are connected (clamping operation), they are connected through the clamping mechanism, and the rotating disk 24 is driven to rotate by controlling the driving mechanism 7. When the rotating disk 24 rotates, the cylindrical rod above the lifting cylinder 23 moves in the inclined groove 35, and the inclined groove 35 squeezes the cylindrical rod, thereby driving the guide block 29 to move in the radial groove, so that multiple lifting cylinders 23 are synchronously moved toward the center of the rotating disk 24. At this time, the pulling line 31 is relaxed and the connecting rod 22 moves downward under the action of its own gravity. The rotating disk 24 continues to rotate, and the cylindrical rod moves in the arc groove 34. At this time, the lifting cylinder 23 no longer moves toward the center, the pulling rope continues to relax, and the connecting rod 22 continues to move downward. At this time, the fixing clamp moves downward, and the lower end of the pulling rope is separated from the lifting cylinder 19.

[0072] At this time (since the rear end of the fixing clip is connected to the pulling rope, it can be conveniently held by hand), it is more convenient to hold the fixing clip by hand to clamp the connection between the lung trachea and the bronchus 20. Clamp the connection of the lung trachea evenly with multiple fixing clips. That is, when clamping, manually pull the moving clip 39 outwards, place the side wall of the lung trachea between the moving clip 39 and the clip body 30, and release the moving clip 39. The moving clip 39 moves inwards under the elastic force of the spring 38 to clamp and fix the side wall of the lung trachea. At this time, control the turntable 24 to rotate in the reverse direction through the controller. When rotating, first, the pulling rope is tightened (when the cylindrical rod moves in the arc-shaped groove 34), the connecting rod 22 moves upwards. The upper end of the connecting rod 22 is a conical structure, and the inner side of the lower end of the lifting cylinder 19 is an inward concave conical structure, which can conveniently place the connecting rod 22 in the lifting cylinder 19 under the drive of the pulling rope. The lower part of the connecting rod 22 and the inside of the lifting cylinder 19 are in clearance fit to prevent the connecting rod 22 from shaking, and at the same time lift the lung trachea.

[0073] The turntable 24 continues to rotate, the cylindrical rod moves in the inclined groove 35, the pulling rope continues to contract, and drives the fixing clip to continue to move upwards; at the same time, the lifting electric cylinder 23 moves outwards, and then drives the fixing clip to move outwards. Multiple fixing clips cooperate at the same time to stretch and expand the connection port of the lung trachea to form a certain opening, which is convenient to put the bronchus 20 into the opening. Then, when controlling the lifting electric cylinder 23 to move upwards through the controller, it drives the lung trachea to move upwards. At this time, the conical structure at the lower end of the bronchus 20 can conveniently enter the opened opening of the lung trachea. The lifting electric cylinder 23 continues to move upwards. Since the lower part of the bronchus 20 is a conical structure, the opening can be quickly and gradually fitted to the bronchus 20. When the opening of the lung trachea is relatively large, the lifting electric cylinder 23 continues to move upwards, so that the opening enters the ball head mechanism, and the ball head structure 21 is used to squeeze and seal the opening to prevent liquid from overflowing during the subsequent perfusion process.

[0074] After the perfusion is completed, control the telescopic electric cylinder 37 to contract through the controller, which drives the limit plate 40 to move, and the limit plate 40 drives the moving clip 39 to move outwards to release the clamping effect on the lung trachea. The lung trachea moves downwards under the action of its own gravity to separate from the bronchus 20 or the ball head structure 21, and then enters the storage cylinder 11 below. The fixing liquid placed in the storage bucket can effectively prevent a large amount of the fixing liquid in the perfused lung tissue from overflowing; and control the lifting mechanism 18 to move downwards to reset the storage box 10, then the storage box 10 can be pulled outwards, which is convenient for subsequent processing of the lung tissue in the storage cylinder 11. Reset all the structures to perform the next round of perfusion operation.

[0075] In the description of the present invention, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0076] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An in vitro lung perfusion device for small animals, comprising a box body, characterized in that: A partition is provided inside the box body. The partition divides the space inside the box body into an operation chamber and a perfusion chamber. The operation chamber is located on the left side of the perfusion chamber. A plurality of perfusion tubes that can move left and right are provided inside the box body. A plurality of branch tubes are provided at the right part of each perfusion tube, and a clamping mechanism is provided at the branch tube. The clamping mechanism is used to clamp the lung. A rotating plate is provided above the partition, and the upper end of the rotating plate is rotatably connected to the inside of the box body; a storage box is provided at the bottom of the perfusion chamber and can move up and down. A storage cylinder corresponding to the branch tube is provided inside the storage box, and each storage cylinder is located directly below the branch tube. A strip-shaped opening is provided on the right side of the box body, and the storage box can be moved out of the box body through the strip-shaped opening.

2. The small animal ex vivo lung perfusion device according to claim 1, wherein: The lower end of the rotating plate is in mutual fit with the upper end of the partition.

3. The small animal ex vivo lung perfusion device according to claim 2, characterized in that: The lower end of the rotating plate is an inclined surface structure inclined to the lower left, and the upper end surface of the partition is in mutual fit with the lower end surface of the rotating plate.

4. The small animal ex vivo lung perfusion device according to claim 1, characterized in that: A strip-shaped groove corresponding to the perfusion tube is provided on the rotating plate, and the perfusion tube can move left and right through the strip-shaped groove.

5. The small animal ex vivo lung perfusion device according to claim 4, wherein: A strip-shaped plate is fixedly provided at the left part of each perfusion tube, and the strip-shaped plate covers the strip-shaped groove.

6. The small animal ex vivo lung perfusion device according to claim 1, characterized in that: A limit block is provided at the upper left side of the upper part of the operation chamber, and each perfusion tube can move left and right through the limit block.

7. The small animal ex vivo lung perfusion device according to claim 1, characterized in that: A driving mechanism is provided at the upper part of the box body. A moving plate is provided at the front end of the driving mechanism. The driving mechanism drives the moving plate to move left and right, and each perfusion tube is fixedly connected to the moving plate.

8. The small animal ex vivo lung perfusion device according to claim 1, characterized in that: A connecting frame is provided at the right part of the perfusion tube, and a plurality of rotatable rollers are evenly distributed on the upper surface of the connecting frame.

9. The small animal ex vivo lung perfusion device according to claim 1, wherein: A lifting mechanism is provided at the bottom of the perfusion chamber, and the lifting mechanism drives the storage box to move up and down.

10. The small animal ex vivo lung perfusion device according to claim 1, wherein: The clamping mechanism includes a plurality of fixed clamps that can move up and down. The fixed clamps can clamp the trachea of the lung. The lower end of the branch tube is a conical structure, and a ball head structure is provided on the branch tube above the conical structure. The conical structure facilitates guiding the trachea of the lung onto the branch tube, and the ball head structure can make the trachea of the lung fit tightly with the branch tube for sealing.

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