Pesticide composition and application thereof

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses a pesticide composition and application thereof, the pesticide composition comprises an active component A and an active component B, the active ingredient A is (4 '-chloro-6-fluoro-4-methyl-[1, 1'-biphenyl]-3-yl) (2, 2, 2-trifluoroethyl) thioether, the active ingredient B is any one of spirodiclofen or bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is (1: 52)-(45: 1). The pesticide composition disclosed by the invention has a very good control effect on citrus spider mites and two-spotted spider mites, and is excellent in fast-acting property and long in lasting period.

Description

[0001] This divisional application of the present invention has an application number of CN202310543087.6, a filing date of May 15, 2023, and an invention title of "A Pesticide Composition and Its Application". Technical Field

[0002] The present invention belongs to the technical field of pesticide acaricides and discloses a pesticide composition and its application. Background Art

[0003] (4'-Chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl)sulfide is a biphenyl sulfide acaricide and has excellent control effects on common plant pest mites. Its structural formula is as follows:

[0004]

[0005] Spirodiclofen is a highly effective and low-toxic acaricide developed by Bayer. It mainly inhibits the fat synthesis in pest mites, blocks energy metabolism, is effective against all development stages of mites, especially has good effects on mite eggs and nymphs, and has a certain inhibitory effect on the reproduction of female adult mites. Its structural formula is as follows:

[0006]

[0007] Bifenazate, with the chemical name of isopropyl 3-(4-methoxybiphenyl-3-yl)hydrazinecarboxylate, this acaricide mainly acts on the Qo site of cytochrome b complex III in the electron transport chain. This drug is effective against all development stages of spider mites. Its structural formula is as follows:

[0008]

[0009] Due to the rough field production methods, agricultural growers use a large amount of insecticides and acaricides, resulting in the field pest mite populations having developed varying degrees of resistance to common acaricides. Therefore, developing acaricides that have a significant synergistic effect on pest mites and can reduce the dosage of pesticides is an important trend in current agricultural development. Summary of the Invention

[0010] Based on the problems in the prior art, the present invention provides a pesticide composition, which has excellent control effects on pest mites, especially has a significant synergistic effect on common two-spotted spider mites and citrus red mites in crops, reduces the dosage of the agent, has excellent quick-acting properties, and a long-lasting effect.

[0011] The present invention also provides various formulation dosage forms of this pesticide composition for controlling two-spotted spider mites and citrus red mites.

[0012] To achieve the above object, the present invention adopts the following technical solutions: A pesticide composition, the pesticide composition contains active ingredient A and active ingredient B, the active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl)sulfide, and the active ingredient B is any one of spirodiclofen or bifenazate;

[0013] Further, the mass ratio of the active ingredient A to the active ingredient B is 1:52 to 45:1;

[0014] Further, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:52 to 44:1;

[0015] The active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:35 to 40:1.

[0016] Even further, the active ingredient B is spirodiclofen, and the mass ratio of the active ingredient A to the active ingredient B is 1:33 to 38:1;

[0017] The active ingredient B is bifenazate, and the mass ratio of the active ingredient A to the active ingredient B is 1:30 to 40:1.

[0018] Further, the total weight of the pesticide composition is calculated as 100 wt%, and the total weight of the active ingredient A and the active ingredient B accounts for 0.1 wt% to 80 wt% of the total weight of the pesticide composition;

[0019] Further, the pesticide composition further includes auxiliary ingredients in addition to the active ingredients, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers;

[0020] Further, the wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, fatty alcohol polyoxyethylene ether sulfates, silkworm excrement, soap pod powder, sapindus powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0021] The dispersant(s) is / are selected from one or more of lignosulfonate, alkylnaphthalenesulfonate formaldehyde condensate, naphthalenesulfonate, triphenylvinylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0022] The thickener(s) is / are selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, carboxymethyl cellulose; and / or

[0023] The disintegrant(s) is / are selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0024] The emulsifier(s) is / are selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenethylphenol polyoxyethylene polypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0025] The defoamer(s) is / are selected from one or more of silicone oil, C 10 ~Csaturated fatty acid compounds, C8-C 20 fatty alcohol compounds or silicone compounds; and / or 10

[0026] The preservative(s) is / are selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate; and / or

[0027] The stabilizer(s) is / are selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, epoxidized vegetable oil; and / or

[0028] The synergist(s) is / are selected from synergistic phosphorus, synergistic ether; and / or

[0029] The carrier(s) is / are selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, silica; and / or

[0030] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0031] All of the above auxiliary components are commercially available.

[0032] Furthermore, the pesticide composition can be prepared into a solid preparation or a liquid preparation;

[0033] Furthermore, the liquid preparation is selected from solution, sol, oil, film-forming oil, emulsifiable concentrate, latex, dispersible concentrate, paste, emulsion in water, oil-in-water emulsion, microemulsion, lipid suspension, microcapsule suspension, oil suspension, dispersible oil suspension, suspension emulsion, microcapsule suspension-suspension, microcapsule suspension-emulsion in water or microcapsule suspension-suspension emulsion; the solid preparation is selected from powder, granule, sphere, tablet, strip, wettable powder, oil-dispersible powder, milk powder, water-dispersible granule, milk granule, water-dispersible tablet, soluble powder, soluble tablet or soluble granule;

[0034] Furthermore, the solid preparation is a water-dispersible granule and a wetting dispersant, and the liquid preparation is a suspension, emulsion in water, microemulsion, emulsifiable concentrate, dispersible oil suspension, suspension emulsion.

[0035] The present invention also discloses the application of the pesticide composition and its preparation as described above in controlling pest mites.

[0036] Furthermore, the pest mites are pest mites of the family Tetranychidae; the pest mites of the family Tetranychidae are Panonychus citri, Panonychus ulmi, Tetranychus urticae or Tetranychus cinnabarinus;

[0037] Even further, the pest mites of the family Tetranychidae are Panonychus citri, Tetranychus urticae.

[0038] The beneficial effects of the present invention are as follows:

[0039] 1. The pesticide composition of the present invention has a significant synergistic effect on common plant pest mites, especially has a significant control effect on Tetranychus urticae and Panonychus citri, can effectively reduce the application frequency of pesticides, and reduce the usage amount of single agents;

[0040] 2. The pesticide composition of the present invention is composed of active ingredients with different action mechanisms, and effectively slows down the generation of pest mite resistance. Specific Embodiments

[0041] In order to make the technical solutions, objectives and advantages of the present invention clearer and more understandable, the present invention is described by the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited by the embodiments described herein.

[0042] Preparation examples of some related preparations:

[0043]

[0044]

[0045] Indoor toxicity determination test

[0046] Example 1: Indoor active ratio screening test

[0047] Test targets: Tetranychus urticae Koch nymphs, Panonychus citri McGregor nymphs.

[0048] Test reference: Conducted according to NY / T 1154.13-2008 "Pesticide Bioassay Guidelines for Indoor Use - Part 13: Leaf Disk Spray Method for Insecticides".

[0049] Test method: Select kidney bean and citrus leaves that have grown uniformly and have not been treated with any pesticides. Use a hole punch to make leaf disks, place them with the back side up in a moisturizing petri dish, and place 2 leaf disks in each petri dish. Then transfer the nymphs to the leaf disks, with 15 nymphs placed on each leaf disk. After the mites are stable, spray them in order from low concentration to high concentration using a Potter spray tower, with 1.5 mL for each treatment, let it settle for 1 minute, and repeat each treatment 4 times. The control area is treated with clean water.

[0050] After treatment, transfer the test mites to a breeding and observation environment at a temperature of (25 ± 1) °C and a photoperiod of L:D = (16:8) h. Repeat each treatment 4 times, and set a treatment without the pesticide (containing all organic solvents and emulsifiers) as the blank control. Check the mortality of the nymphs 48 hours after the pesticide treatment, and record the total number of mites and the number of dead mites respectively.

[0051] Statistics and analysis:

[0052] Based on the survey data, calculate the mortality of each treatment. Calculate according to the following formula:

[0053]

[0054] In the formula:

[0055] P - Mortality rate, in percentage (%);

[0056] K - Represents the number of dead insects, in number;

[0057] N - Represents the total number of insects in the treatment, in number.

[0058]

[0059] Wherein:

[0060] P1——Corrected mortality rate, unit: percentage (%)

[0061] P t ——Mortality rate of treatment, unit: percentage (%)

[0062] P0——Mortality rate of blank control, unit: percentage (%)

[0063] If the control mortality rate < 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the corrected mortality rate formula; if the control mortality rate > 20%, the test needs to be redone

[0064] Analyze the obtained test data results using the DPS statistical analysis system to obtain the virulence regression equation, correlation coefficient, and LC 50 value, and evaluate the activity of the test agent against the biological test material

[0065] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows

[0066]

[0067] Wherein:

[0068] ATI——Actual virulence index of the mixture

[0069] S——LC of the standard acaricide 50 , unit: milligram per liter (mg / L)

[0070] M——LC of the mixture 50 , unit: milligram per liter (mg / L)

[0071] TTI = TI A ×P A +TI B ×P B

[0072] Wherein:

[0073] TTI——Theoretical virulence index of the mixture

[0074] TI A ——Virulence index of agent A

[0075] P A ——Percentage content of agent A in the mixture, unit: percentage (%)

[0076] TI B ——Virulence index of agent B

[0077] P B ——Percentage content of Agent B in the mixture, in percentage (%)

[0078]

[0079] Wherein:

[0080] CTC——Coefficient of co-toxicity

[0081] ATI——Actual toxicity index of the mixture

[0082] TTI——Theoretical toxicity index of the mixture

[0083] When the coefficient of co-toxicity CTC of the compound ≥ 120, it shows a synergistic effect; when CTC ≤ 80, it shows an antagonistic effect; when 80 < CTC < 120, it shows an additive effect

[0084] The results of relevant indoor activity tests are shown in the following table

[0085] Table 1 Indoor bioactivity test of the compound of active ingredient A and spirodiclofen against Tetranychus urticae nymphs

[0086]

[0087]

[0088] The indoor activity test is shown in Table 1. The compound of active ingredient A and spirodiclofen has an obvious synergistic effect on Tetranychus urticae within a reasonable range. The mass ratio of active ingredient A to spirodiclofen is 1:52 to 44:1, and the coefficient of co-toxicity is greater than 120, and the combined effect shows a synergistic effect

[0089] Table 2 Indoor bioactivity test of the compound of active ingredient A and bifenazate against Tetranychus urticae nymphs

[0090]

[0091] The results of the indoor activity test are shown in Table 2. When the compound of active ingredient A and bifenazate has a mass ratio of 1:30 to 40:1, the coefficient of co-toxicity is greater than 120, and it shows a synergistic effect on Tetranychus urticae

[0092] Table 3 Indoor bioactivity determination test of the compound of active ingredient A and spirodiclofen against Panonychus citri nymphs

[0093]

[0094]

[0095] The results of indoor bioactivity tests are shown in Table 3. The mass ratio of active ingredient A to spirodiclofen is 1:33 to 38:1, and the co-toxicity coefficient is greater than 120, indicating a synergistic effect on Panonychus citri McGregor.

[0096] Table 4 Indoor Bioactivity Test of the Compound of Active Ingredient A and Bifenazate against Nymphs of Panonychus citri McGregor

[0097]

[0098] The results of indoor bioactivity tests are shown in Table 4. The mass ratio of active ingredient A to bifenazate is 1:35 to 40:1, and the co-toxicity coefficient is greater than 120, indicating a synergistic effect on Panonychus citri McGregor.

[0099] Field Efficacy Verification Test

[0100] Example 2: Field Control Test of Tetranychus urticae Koch on Luffa

[0101] This test was conducted with reference to GB / T 17980.17-2000 Guidelines for Field Efficacy Trials of Pesticides (Part 1) Acaricides against Tetranychus spp. on Beans and Vegetables.

[0102] The test was carried out in Zhangqiao Village, Shouguang City, Shandong Province, where the soil fertility was medium to high.

[0103] Test crop: Luffa.

[0104] Test target: Tetranychus urticae Koch.

[0105] Test design: The treatments of the test plots, control plots, and blank control plots were arranged in a randomized block design. The cultivation conditions of all test plots were uniform and consistent with local scientific agricultural practices. The area of each test plot was 30m 2 , and each treatment was replicated 4 times.

[0106] Application time: The pesticide was applied once on September 20, 2022, with a water consumption of 45 kg per mu. Spraying was carried out once when the mites reached the control index (i.e., when there were 3-10 adult and nymph mites per leaf on about 10% of the leaves).

[0107] Test investigation time: 20 leaves were randomly selected from each plot to count the number of adult and nymph live mites. The base number was investigated before the application, and the investigation was carried out 3 days, 7 days, and 14 days after the application.

[0108] Efficacy calculation method:

[0109]

[0110] Table 5 Results of Field Control Test of Tetranychus urticae Koch on Luffa

[0111]

[0112] The results of the field efficacy test for controlling two-spotted spider mites show (Table 5) that the pesticide composition of the present invention has a good control effect on two-spotted spider mites of Luffa, and has good rapidity and a long lasting effect.

[0113] Example 3: Field test for controlling citrus spider mites

[0114] Refer to GB / T 17980.11-2000 "Guidelines for field efficacy tests of pesticides (I) Acaricides for the control of Panonychus citri".

[0115] Test location: This test was conducted in Shuangqiao Town, Wuming District, Nanning City, Guangxi Zhuang Autonomous Region. The tree ages of plants in each plot of the test site were consistent, and the crown sizes were basically the same.

[0116] Experimental crops: citrus.

[0117] Test target: citrus spider mite.

[0118] Test drug: The test plot area has 2 fruit trees, and each treatment is repeated 4 times. The plot treatments of test drug, control drug and blank control are arranged in random blocks.

[0119] Application time: Apply the pesticide once on October 28, 2022. Apply 1.5L of pesticide per plant. Use an electric sprayer to apply the pesticide to the whole citrus tree. The spraying should be done when the leaves are wet but not dripping.

[0120] Survey method: In each plot, young shoots were marked at the east, west, south, north and center of the tree, and the number of active mites on 20 leaves was surveyed. The leaf surface was directly observed with a handheld magnifying glass to count the number of mites.

[0121] Survey time and frequency: Survey once every 3 days and 15 days after treatment, and record the number of active mites.

[0122] Calculation method of drug efficacy:

[0123]

[0124]

[0125] The results of the field efficacy test are shown in the following table:

[0126] Table 6 Results of field trials on citrus spider mites

[0127]

[0128] The results of the field efficacy control test (Table 6) show that the pesticide composition of the present invention has a good control effect on citrus red spider mites. 3 days after the application, the control efficacy of Preparation Example 2 and Preparation Example 5 on citrus spider mites is higher than 90%.

[0129] No phytotoxicity to the target crops was found during the above field trials, nor were there any adverse effects on non-target organisms.

[0130] Although the present invention has been described in detail above with general descriptions and specific embodiments, modifications or improvements can be made thereto based on the present invention, which will be obvious to those skilled in the art. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of the present invention claimed.

Claims

1. A pesticide composition, characterized in that, The described pesticide composition comprises active ingredient A and active ingredient B. Active ingredient A is (4'-chloro-6-fluoro-4-methyl-[1,1'-biphenyl]-3-yl)(2,2,2-trifluoroethyl)sulfide, and active ingredient B is bifenazate.

2. The pesticide composition according to claim 1, wherein The mass ratio of active ingredient A to active ingredient B is 1:52 to 45:

1.

3. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A to active ingredient B is 1:35 to 40:

1.

4. The pesticide composition according to claim 3, wherein The mass ratio of active ingredient A to active ingredient B is 1:30 to 40:

1.

5. The pesticide composition according to claim 1, characterized in that, Based on 100 wt% of the total weight of the described pesticide composition, the total weight of active ingredient A and active ingredient B accounts for 0.1 wt% to 80 wt% of the total weight of the pesticide composition.

6. The pesticide composition according to claim 1, characterized in that, In addition to the active ingredients, the described pesticide composition also includes auxiliary ingredients, which are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

7. The pesticidal composition according to claim 1, characterized in that, The preparation dosage form of the described pesticide composition can be a solid preparation or a liquid preparation.

8. The pesticide composition according to claim 1, wherein The solid preparation is a water dispersible granule or a wetting dispersant, and the liquid preparation is a suspension concentrate, an emulsion in water, a microemulsion, an emulsifiable concentrate, a dispersible oil suspension or a suspoemulsion.

9. Use of the pesticide composition according to any one of claims 1-8 for controlling harmful mites.

10. The application according to claim 9, characterized in that, The harmful mites are mites of the family Tetranychidae; the mites of the family Tetranychidae are Panonychus citri, Panonychus ulmi, Tetranychus urticae or Tetranychus cinnabarinus; Preferably, the mites of the family Tetranychidae are Panonychus citri or Tetranychus urticae.

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