Pesticide composition containing cyantraniliprole and application thereof

Abstract

The invention belongs to the technical field of pesticide disinfestation, and discloses a pesticide composition containing cyantraniliprole, the pesticide composition comprises an active component A, an active component B and an active component C. The active component A is cyantraniliprole, the active component B is lambda-cyhalothrin, and the active component C is an active component B. The active component A is cyantraniliprole. The active ingredient C is any one of clothianidin and thiamethoxam. The pesticide composition disclosed by the invention can effectively prevent and treat various plant underground pests and seedling stage pests, is particularly efficient for peanut and corn underground pests and seedling stage pests, enhances the stress resistance of plants, can reduce the dosage, has a long lasting period, and has no phytotoxicity to crops.

Description

[0001] This invention application is a divisional application with application number 202310979202.4, application date August 5, 2023, and invention name “A pesticide composition containing cyantraniliprole and its use”. Technical Field

[0002] The invention belongs to the technical field of pesticides and specifically discloses a pesticide composition containing cyantraniliprole and uses thereof. Background Art

[0003] Cyantraniliprole is an anthranilamide insecticide developed by DuPont. It effectively activates insect ryanodine receptors, causing calcium ion release channels to continuously open within the insect's striated and smooth muscle cells, rapidly reducing intraluminal calcium levels and paralyzing the insects, leading to their death. It has a strong inhibitory insecticidal effect and good systemic properties, allowing it to be transported throughout plants.

[0004] Lambda-Cyhalothrin, also known as λ-cyhalothrin, mainly acts as a contact and stomach poison. It can achieve the effect of pest control by inhibiting insect nerve conduction. It mainly controls various pests such as aphids on crops such as cotton, cruciferous vegetables, fruit trees and tea trees.

[0005] Neonicotinoids are a class of highly effective, safe, and selective insecticides. They selectively target nicotinic acetylcholinesterase receptors in the insect nervous system, blocking the normal conduction of the insect's central nervous system, causing paralysis and death. Neonicotinoids are effective against pests of the Homoptera, Hemiptera, Coleoptera, and Lepidoptera orders, and are safe for mammals and the environment. They can be used for stem and foliage treatments, as well as soil and seed treatments.

[0006] The present invention rationally compounds cyantraniliprole, high-efficiency chlorflucythrin, and either one of the neonicotinoid insecticides thiamethoxam or clothianidin, studies the safety of crop seedlings, the effects on seedling quality, and the control effects on plant diseases, determines the optimal ratio and dosage of the seed dressing agent, and provides a scientific basis for screening out safe and efficient seed dressing agents and promoting their large-scale application in production. Summary of the Invention

[0007] Based on the above situation, the purpose of the present invention is to provide a pesticide composition containing cyantraniliprole, which can effectively prevent and control a variety of underground pests and seedling pests of plants, especially underground pests and seedling pests of peanuts and corn. It is highly effective, requires a small amount of medicine, has a long lasting effect, and has no phytotoxicity to crops, and can significantly improve the stress resistance of crops.

[0008] In order to achieve the above object, the technical solution adopted by the present invention is as follows:

[0009] A pesticidal composition containing cyantraniliprole, said pesticidal composition comprising active ingredient A, active ingredient B and active ingredient C, wherein said active ingredient A is cyantraniliprole, said active ingredient B is lambda-cyhalothrin, and said active ingredient C is any one of thiamethoxam or clothianidin;

[0010] Further, the mass ratio of active ingredient A, active ingredient B and active ingredient C is (1 - 20):(1 - 15):(1 - 20);

[0011] Further, the mass ratio of active ingredient A, active ingredient B and active ingredient C is (1 - 15):(1 - 10):(1 - 15);

[0012] Further, the mass ratio of active ingredient A, active ingredient B and active ingredient C is (1 - 10):(1 - 9):(1 - 10);

[0013] Further, the mass ratio of active ingredient A, active ingredient B and active ingredient C is 2:1:2, 2:1:6, 2:1:10, 1:1:1, 1:1:3, 1:1:5, 1:2:1, 1:2:3, 1:2:5, 6:1:2, 6:1:6, 6:1:10, 3:1:1, 3:1:3, 3:1:5, 3:2:1, 3:2:3, 3:2:5, 10:1:2, 10:1:6, 10:1:10, 5:1:1, 5:1:3, 5:1:5, 5:2:1, 5:2:3, 5:2:5, 3:1:6, 3:1:9, 3:5:3, 3:5:6, 3:5:9, 1:3:1, 1:3:2, 1:3:3, 5:1:3, 5:1:6, 5:1:9, 5:5:3, 5:5:6, 5:5:9, 5:9:3, 5:9:6, 5:9:9, 7:1:3, 7:1:6, 7:1:9, 7:5:3, 7:5:6, 7:5:9, 7:9:3, 7:9:6, 7:9:9.

[0014] Further, based on the total weight of the pesticidal composition being 100 wt%, the active ingredients account for 1% - 80% of the pesticidal composition;

[0015] Further, based on the total weight of the pesticidal composition being 100 wt%, the active ingredients account for 2% - 80% of the pesticidal composition;

[0016] Further, the pesticidal composition further comprises adjuvants, and the adjuvants are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreezing agents, defoaming agents, solvents, preservatives, stabilizers, warning colors, film-forming agents, synergists and carriers;

[0017] The wetting agent is selected from one or more of alkylbenzene sulfonates, alkylnaphthalene sulfonates, lignin sulfonates, sodium dodecyl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonates, alkylphenol polyoxyethylene ethers, castor oil polyoxyethylene ethers, alkylphenol ethoxylates, fatty alcohol ethoxylates, sodium fatty alcohol polyoxyethylene ether sulfate, silkworm excrement, sophora powder, sapindus powder, SOPA, detergent, emulsifier series 2000, and wetting penetrant F; and / or

[0018] The dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, triphenylvinylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether methyl ether condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylates, phosphates, EO-PO block copolymers, and EO-PO graft copolymers; and / or

[0019] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, and alkylphenol ether phosphate; and / or

[0020] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose, and white carbon black; and / or

[0021] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid, and tartaric acid; and / or

[0022] The antifreeze is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons, and inorganic salts; and / or

[0023] The defoamer is selected from C 10 -C 20 saturated fatty acid compounds, silicone oils, silicone compounds, C8-C 10 fatty alcohols; and / or

[0024] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel oil, solvent naphtha, vegetable oil, vegetable oil derivatives, and water; and / or

[0025] The preservative is selected from one or more of propionic acid, sodium propionate, sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, methyl p-hydroxybenzoate, Kathon and 1,2-benzisothiazolin-3-one; and / or

[0026] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc powder, montmorillonite and starch; and / or

[0027] The warning color is selected from one or more of blue, green, red, purple and their adjusted colors; and / or

[0028] The film-forming agent is selected from one or more of sodium carboxymethyl starch, cellulose derivatives (sodium carboxymethyl cellulose, sodium alginate, polyvinyl alcohol), polyacrylic acid; and / or

[0029] The synergist is selected from synergistic phosphorus, synergistic ether; and / or

[0030] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0031] Furthermore, the pesticide composition can be prepared into any preparation dosage form acceptable in agriculture, and the preparation dosage form is any one of seed treatment dry powder, seed treatment dispersible powder, seed treatment liquid, seed treatment emulsion or seed treatment suspension;

[0032] Even further, the preparation dosage form is seed treatment suspension.

[0033] The present invention also discloses the use of the pesticide composition as described above for controlling plant pests.

[0034] Furthermore, the plants include corn, wheat, peanut, rice and / or cotton; the pests are cutworms, white grubs, aphids, corn borers, striped stem borers, wireworms, thrips and / or Athetis lepigone;

[0035] Furthermore, the plants are corn and / or peanut, and the pests are cutworms and / or white grubs.

[0036] Furthermore, the pesticide composition is applied to plants, plant propagation materials or subsequently grown plant organs, cultivation media, cultivation materials or cultivation spaces at an effective dosage application rate.

[0037] The beneficial effects of the present invention are as follows:

[0038] 1) The pesticide composition of the present invention can be used to control various underground pests and seedling pests of plants, reducing the dosage of pesticides and being safe for the environment and crops.

[0039] 2) The pesticide composition of the present invention can effectively slow down the generation of resistance in target pests. One application in the field shows obvious control effect and a long residual period. Detailed implementation manners

[0040] To make the technical solutions, objectives and advantages of the present invention clearer and more understandable, the present invention is described with the following specific examples, but the present invention can be implemented in various forms and should not be limited by the implementation manners described herein.

[0041] Formulation preparation examples

[0042] Preparation method of seed treatment suspension: According to the ratio, the active ingredients, auxiliaries and water are mixed and stirred evenly by high-shear shearing, and then sanded for 2.5 h by a sand mill to make the average particle size reach 1-5 microns. Finally, a thickener, a preservative and a film-forming agent are added and stirred evenly by shearing to obtain the seed treatment suspension.

[0043] Preparation example 1: 35% Cyantraniliprole · Lambda-cyhalothrin · Thiamethoxam seed treatment suspension (3:1:3)

[0044] Component Content Cyantraniliprole 15％ Lambda-cyhalothrin 5％ Thiamethoxam 15％ Fatty amine polyoxyethylene ether 4％ Alkylphenol polyoxyethylene ether phosphate salt 2％ Sodium lignosulfonate 2％ Sodium benzoate 1％ Xanthan gum 0.1％ Rose red pigment 5％ Propylene glycol 5％ Magnesium aluminum silicate 0.5％ Organosilicon defoamer 0.5％ Polyacrylic acid 1％ Deionized water Make up the balance

[0045] Preparation example 2: 35% Cyantraniliprole · Lambda-cyhalothrin · Clothianidin seed treatment suspension (3:1:3)

[0046] Component Content Cyantraniliprole 15％ Lambda-cyhalothrin 5％ Clothianidin 15％ Phenethylphenol polyoxyethylene polyoxypropylene ether 3％ Alkylphenol formaldehyde resin polyoxyethylene ether 4％ Sodium alginate 2％ Sodium carboxymethyl starch 1％ Xanthan gum 0.1％ Rose red pigment 5％ Propylene glycol 5％ Magnesium aluminum silicate 1％ Organosilicon defoamer 0.5％ Sodium benzoate 1％ Deionized water Make up the balance

[0047] Preparation example 3: 44% Cyantraniliprole · Lambda-cyhalothrin · Thiamethoxam seed treatment suspension (5:1:5)

[0048]

[0049]

[0050] Preparation example 4: 44% Cyantraniliprole · Beta-cyfluthrin · Clothianidin seed treatment suspension (5:1:5)

[0051] Component Content Cyantraniliprole 20％ Lambda-cyhalothrin 4％ Clothianidin 20％ Isotridecanol polyoxyethylene ether 2％ Alkylphenol formaldehyde resin polyoxyethylene ether 2％ Sodium lignosulfonate 3％ Polyvinyl alcohol 2％ Xanthan gum 0.2％ Rose red pigment 7％ Glycerol 5％ Magnesium aluminum silicate 1％ Organosilicon defoamer 1％ Kathon 2％ Deionized water Make up the balance

[0052] Preparation example 5: 45% Cyantraniliprole · Beta-cyfluthrin · Thiamethoxam seed treatment suspension (2:1:2)

[0053] Component Content Cyantraniliprole 18％ Lambda-cyhalothrin 9％ Thiamethoxam 18％ Alkylphenol polyoxyethylene ether phosphate 3％ Naphthalene sulfonate formaldehyde condensate 2％ Polyacrylic acid 1％ Xanthan gum 0.3％ Rose red pigment 5％ Ethylene glycol 5％ Magnesium aluminum silicate 1％ Organosilicon defoamer 0.5％ Sodium benzoate 1％ Deionized water Make up the balance

[0054] Preparation Example 6: 45% cyantraniliprole·beta-cyfluthrin·clothianidin seed treatment suspension concentrate (2:1:2)

[0055]

[0056]

[0057] Control Example 1: 32% cyantraniliprole·lambda-cyhalothrin seed treatment suspension concentrate (3:1)

[0058] Component Content Cyantraniliprole 24％ Lambda-cyhalothrin 8％ Fatty amine polyoxyethylene ether 3％ Alkylphenol polyoxyethylene ether phosphate salt 2％ Sodium lignosulfonate 2％ Sodium benzoate 1％ Xanthan gum 0.1％ Rose red pigment 5％ Propylene glycol 5％ Magnesium aluminum silicate 0.5％ Organosilicon defoamer 0.5％ Polyacrylic acid 1％ Deionized water Make up the balance

[0059] Control Example 2: 40% cyantraniliprole·thiamethoxam seed treatment suspension concentrate (1:1)

[0060]

[0061]

[0062] Control Example 3: 32% lambda-cyhalothrin·thiamethoxam seed treatment suspension concentrate (1:3)

[0063] Component Content Thiamethoxam 24％ Lambda-cyhalothrin 8％ Fatty amine polyoxyethylene ether 3％ Alkylphenol polyoxyethylene ether phosphate salt 3％ Sodium lignosulfonate 3％ Sodium benzoate 1％ Xanthan gum 0.2％ Rose red pigment 5％ Propylene glycol 5％ Magnesium aluminum silicate 0.5％ Organosilicon defoamer 0.5％ Polyacrylic acid 1％ Deionized water Make up the balance

[0064] Control Example 4: 40% cyantraniliprole·clothianidin seed treatment suspension concentrate (1:1)

[0065] Component Content Cyantraniliprole 20％ Clothianidin 20％ Phenethylphenol polyoxyethylene polyoxypropylene ether 4％ Alkylphenol formaldehyde resin polyoxyethylene ether 5％ Sodium dodecyl sulfate 2％ Sodium carboxymethyl starch 2％ Xanthan gum 0.5％ Rose red pigment 5％ Propylene glycol 5％ Magnesium aluminum silicate 1％ Organosilicon defoamer 1％ Sodium benzoate 2％ Deionized water Make up the balance

[0066] Control Example 5: 36% highly effective chlorflucythrin·clothianidin seed treatment suspension concentrate (1:3)

[0067]

[0068]

[0069] Indoor bioassay test:

[0070] Example 1: Indoor bioassay test on white grubs treated with different agents

[0071] The test was conducted in accordance with NY / T 1154.6-2006 “Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticide Activity Test - Insect Immersion Method”.

[0072] Test target: The grubs were the second-instar larvae of the black beetle (Holotrichia oblita F.).

[0073] Experimental instruments and equipment: electronic balance (sensitivity 0.1 mg), pipette, insect immersion cage, culture dish, graduated cylinder, filter paper, marker pen, stirring rod, stopwatch, beaker, brush, tweezers, etc.

[0074] Experimental Design

[0075] 1) Test agents: Thiamethoxam technical, content: 98%; Clothianidin technical, content: 96%; Cyantraniliprole technical, content: 94%; Lambda-cyhalothrin technical, content: 95%. All the above test agents were provided by the R & D Center of Hailir Pharmaceutical Group.

[0076] 2) Agent preparation: The above agents were separately dissolved in acetone and then diluted with an aqueous solution of 0.1% Tween. According to the activity of the agents, 5 mass concentration gradients were set by the geometric progression method.

[0077] 3) Agent treatment

[0078] The dipping method was used for indoor bioassay. Second-instar larvae of Holotrichia oblita with consistent growth were selected. After investigating the base number of each treatment, the target test insects were immersed in the liquid medicine for 10 s, and then the excess liquid medicine was blotted with filter paper. The test insects were transferred to a glass tube containing fresh peanut leaves or a small amount of potato pieces as food, and the tube mouth was covered with a damp black cloth for feeding. Each concentration had 4 replicates, with 15 test insects in each replicate, and a treatment without the agent was set as the blank control. They were placed in an artificial climate chamber at 25 ± 1°C, relative humidity of (70 ± 10)%, and a photoperiod of 16 h:8 h (light:dark) for feeding.

[0079] Data investigation

[0080] The death situation of the test insects was investigated 48 h after the agent treatment. The standard for judging the death of the test insects was that the body of the insect shrank significantly or could not move normally when pricked with a needle, and the number of dead insects was recorded.

[0081] Data statistics and analysis:

[0082] According to the investigation results, the mortality rate and corrected mortality rate of each treatment concentration on the test insects were calculated according to the mortality rate calculation formula, with the unit of percentage (%).

[0083]

[0084]

[0085] If the control mortality rate < 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the corrected mortality rate formula; if the control mortality rate > 20%, the test needs to be redone.

[0086] The expected value of the composition and its activity was calculated according to the following formula

[0087]

[0088] In the formula:

[0089] X—the mortality rate when the dosage of agent 1 is P;

[0090] Y—the mortality rate when the dosage of agent 2 is Q;

[0091] Z—the mortality rate when the dosage of agent 3 is R.

[0092] In order to show the degree of synergistic effect, the expected value is calculated based on the Colby method. When the measured value is greater than the expected value, it is determined that there is a synergistic effect.

[0093] The results of the indoor test are shown in Table 1 and Table 2.

[0094] Table 1 Indoor bioassay test results of the mixture of cyantraniliprole + lambda-cyhalothrin + thiamethoxam against white grubs

[0095]

[0096]

[0097] Table 2 Indoor bioassay test results of the mixture of cyantraniliprole + lambda-cyhalothrin + clothianidin against white grubs

[0098]

[0099]

[0100] Example 2: Indoor bioassay test of different agent treatments against Agrotis ypsilon

[0101] This test was carried out with reference to NY / T 1154.14-2008 "Pesticide indoor bioassay test guidelines Insecticides Part 14: Leaf-dipping method".

[0102] Test target: Agrotis ypsilon, third-instar larvae, provided by the Group Bioassay Center Laboratory.

[0103] Test steps:

[0104] 1) Test agents: Thiamethoxam technical, content: 98%; Clothianidin technical, content: 96%; Cyantraniliprole technical, content: 94%; Lambda-cyhalothrin technical, content: 95%. All the above test agents were provided by the Group R & D Center.

[0105] 2) Preparation of medicaments: Dissolve the above test medicaments in acetone respectively, and then dilute them with an aqueous solution of 0.1% Tween. According to the activity of the medicaments, set up 5 mass concentration gradients by the method of geometric progression.

[0106] 3) Preparation of test materials: Select standard test insects with consistent physiological states; select cabbage leaves that have not been treated with drugs. Wash the waxy layer on the surface of the cabbage leaves, dry them, and then use a hole punch to punch the cabbage leaves into leaf discs for standby.

[0107] 4) Medicament treatment: Immerse the leaf discs in the test liquid to be measured, take them out and dry them after 10 s, place them in a petri dish containing moisturizing filter paper, and introduce the test insects. Each treatment is repeated 4 times, with 20 test insects in each repetition. And set up a treatment without medicament as a blank control.

[0108] 5) Rearing and observation: Raise and observe the treated test insects under the conditions of temperature (25±1)°C, relative humidity (75±5)%, and photoperiod 16L:8D. Check the results after 72 h. Those that show no response when gently touched with forceps are regarded as dead. Record the total number of insects and the number of dead insects respectively.

[0109] Data statistics and analysis:

[0110] According to the investigation results, calculate the mortality rate and corrected mortality rate of each treatment concentration on the test insects according to the mortality rate calculation formula, and the unit is percentage (%).

[0111]

[0112]

[0113] If the control mortality rate < 5%, no correction is required; if the control mortality rate is between 5% - 20%, correction should be made according to the corrected mortality rate formula; if the control mortality rate > 20%, the test needs to be redone.

[0114] The expected value of the composition and its activity is calculated according to the following formula

[0115]

[0116] In the formula:

[0117] X—the mortality rate when the dosage of medicament 1 is P;

[0118] Y—the mortality rate when the dosage of medicament 2 is Q;

[0119] Z—the mortality rate when the dosage of medicament 3 is R.

[0120] In order to show the degree of synergistic effect, the expected value is calculated based on the Colby method. When the measured value is greater than the expected value, it is determined that there is a synergistic effect.

[0121] The results of the laboratory tests are shown in Tables 3 and 4.

[0122] Table 3 Results of the indoor bioassay of chlorantraniliprole + lambda-cyhalothrin + thiamethoxam against Agrotis ypsilon

[0123]

[0124]

[0125] Table 4 Results of the indoor bioassay of chlorantraniliprole + lambda-cyhalothrin + clothianidin against Agrotis ypsilon

[0126]

[0127]

[0128] Field trial

[0129] Example 3: Effects of different pesticide treatments on the emergence, growth and development of maize

[0130] The pesticides tested and their application rates are shown in the following table:

[0131] Table 5 Pesticides tested and their application rates

[0132]

[0133] Test crop: Maize (Xianyu 335), provided by the Group Bioassay Center Laboratory.

[0134] Experimental design:

[0135] 1) Seed treatment: Select plump maize seeds, mix the seeds with a seed coating agent, dry them and set aside. Use the seeds without pesticide treatment as the blank control for indoor seed viability determination and field trials.

[0136] 2) Indoor seed vigor determination: The test was carried out according to the standard germination test of GB / T 3543.5-1995. Select 400 seeds from the coated seeds of each treatment, with 100 seeds per replicate and 4 replicates. Sow the seeds in germination boxes (sand bed, constant temperature 25 °C), and count the germination rate on the 7th day of the test.

[0137]

[0138] 3) Field trial: Sow the seeds of each treatment in the field according to the experimental treatment. The area of each plot is 30 m 2 , with 4 replicates for each treatment and 200 seeds per replicate. Dig ditches manually before sowing and sow seeds one by one.

[0139] On day 21 after emergence, 10 seedlings were randomly selected from each replicate to measure physiological indices such as plant height, number of roots, root activity, and peroxidase activity.

[0140] Physiological index determination method: The test determination method refers to Gong Fusheng's "Plant Physiology Experiment", the root activity is determined by the TTC method, and the peroxidase activity is determined by the guaiacol method.

[0141] Table 6 Effects of different pesticide treatments on seed germination rate and seedling growth and development

[0142]

[0143] It can be seen from the test results in the above table that different seed coating agents have no significant effect on the germination rate of corn seeds and are safe for crops. Coating treatment can promote corn growth. Compared with the blank control, the plant height of seeds coated with the seed coating agent of the present invention is significantly increased; in addition to the growth of the aboveground part, the seed coating agent of the present invention can also significantly promote root development, significantly improve root activity, and enhance the lodging resistance of corn; the peroxidase activity (POD) measurement results show that after the seeds are coated with the seed coating agent of the present invention, the POD activity in the plant body is significantly increased, indicating that the seed coating agent of the present invention has an enhancing effect on POD in the corn plant body, accelerates the decomposition of hydrogen peroxide in the corn body, and improves the stress resistance of the corn plant.

[0144] Example 4: Field efficacy test of different pesticides for controlling corn cutworms

[0145] The test was conducted in accordance with GB / T 17980.72-2004 "Guidelines for Field Efficacy Tests of Pesticides (II) Part 72: Insecticides for Control of Dryland Subterranean Pests".

[0146] The experiment was conducted in a corn field in Zhecheng County, Shangqiu City, Henan Province. The soil was sandy loam and irrigated after sowing. The soil had medium fertility and the previous crop was wheat.

[0147] Experimental crop: corn (Xianyu No. 335).

[0148] The test drugs and their dosages are shown in Table 7.

[0149] Test Methods: Before sowing, each treatment agent was thoroughly mixed with the seeds according to the dosage, then air-dried and set aside. A completely randomized design was used, with 12 treatments and four replicates per treatment. A fixed amount of 100 seeds were sown in each plot. The test was conducted at the four-leaf stage of corn. Grub infestation was investigated using a five-point "Z" sampling method, with five points selected per plot and 10 plants surveyed at each point, for a total of 50 plants. The number of damaged plants was counted, and the damage rate and control efficacy were calculated.

[0150] Safety investigation: From the time peanuts were sown and seedlings emerged to before harvest, the field growth of peanuts was observed at irregular intervals, and no obvious symptoms of pesticide damage were found.

[0151] The calculation method of prevention effect is:

[0152]

[0153] Table 7 Field efficacy test results of different pesticides for controlling corn cutworms

[0154]

[0155] It can be seen from the field efficacy test in Table 7 that the three-component mixed pesticide of the present invention has a good control effect on corn cutworms and reduces the diseased plant rate.

[0156] Example 5: Field efficacy test of different pesticides for controlling peanut grubs

[0157] The trial was conducted in a peanut field in Yuanshang Town, Laixi City, Shandong Province. This field is a continuously cropped peanut field, and groundnut pests have been a serious problem for years. Artificial ridges were used, and soil, cultivation, and water and fertilizer management conditions were consistent across all experimental plots.

[0158] Peanut used for testing: Huayu 22.

[0159] Test subject: white grub.

[0160] Application time: Conventional seed dressing and pesticide application were performed 1 day before sowing. The sowing date was May 6, 2021. The test plots were irrigated before sowing, and sowing began when the plots were ready for planting. Two peanut seeds were sown in each hole, with a hole spacing of 16 cm and a row spacing of 40 cm. The seeds were then covered with soil.

[0161] Experimental design: The experiment adopts random block arrangement, with each plot of 30m 2 , each treatment was repeated 4 times.

[0162] Seedling emergence: 50 holes were randomly selected in each plot, the emergence of peanuts was recorded, the emergence rate was calculated, and the growth of peanuts was observed.

[0163] Insect population density: When peanuts were harvested, samples were taken at 10 points in a "Z" shape in each treatment plot. The excavated area at each point was 50 cm long × 50 cm wide × 30 cm deep, and the number of remaining insects was recorded separately.

[0164] Damage Index: When harvesting peanuts, sample 5 points in a "Z" shape in each treatment plot. Ten peanut plants are surveyed at each point, and their pods are graded using a 5-level grading standard:

[0165] Level 0: Pods are intact and show no signs of damage;

[0166] Level 1: The peel is damaged, but the kernel is intact;

[0167] Level 2: less than 1 / 2 of the pods are damaged;

[0168] Level 3: 1 / 2 to 3 / 4 of the pods are affected;

[0169] Level 4: More than 3 / 4 of the pods are damaged, and the number of damaged peanut pods at each level is counted.

[0170] From the time peanuts were sown and seedlings emerged to before harvest, the field growth of peanuts was observed at irregular intervals, and no obvious symptoms of pesticide damage were found.

[0171] Calculation method of drug efficacy:

[0172]

[0173] Table 8 Field efficacy test results of different pesticides for controlling peanut grubs

[0174]

[0175]

[0176] From the control effects in Table 8, it can be seen that the three-way mixed pesticide showed obvious control effect on peanut white grubs. Compared with the blank control pesticide treatment, the disease index was reduced, and it had good insect control and control effects.

[0177] Indoor toxicity assays and field trials have shown that the combination of cyantraniliprole, lambda-cyhalothrin, and thiamethoxam or clothianidin demonstrates excellent control effectiveness against underground pests such as cutworms and white grubs. The resulting pesticide composition or formulation exhibits significant control efficacy, significantly increasing plant stress resistance and outperforming single agents in delaying the development of resistance and prolonging drug retention. Furthermore, the combination is safe and well-tolerated for crop seeds.

[0178] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A pesticidal composition containing cyantraniliprole, characterized in that, The described pesticide composition comprises active ingredient A, active ingredient B, and active ingredient C. Active ingredient A is cyantraniliprole, active ingredient B is lambda-cyhalothrin, and active ingredient C is thiamethoxam.

2. The pesticide composition according to claim 1, characterized in that, The mass ratio of active ingredient A, active ingredient B, and active ingredient C is (1 - 20):(1 - 15):(1 - 20).

3. The pesticide composition according to claim 1, wherein The mass ratio of active ingredient A, active ingredient B, and active ingredient C is (1 - 15):(1 - 10):(1 - 15).

4. The pesticidal composition according to claim 1, characterized in that, The mass ratio of active ingredient A, active ingredient B, and active ingredient C is (1 - 10):(1 - 9):(1 - 10).

5. The pesticide composition according to claim 1, characterized in that, Based on the total weight of the pesticide composition being 100 wt%, the active ingredients account for 1% - 80% of the pesticide composition, preferably 2% - 70%.

6. The pesticide composition according to claim 1, wherein The pesticide composition further comprises adjuvants, and the adjuvants are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, warning colors, film-forming agents, synergists, and carriers.

7. The pesticide composition according to claim 1, characterized in that, The described pesticide composition can be prepared into any agriculturally acceptable formulation dosage form, and the formulation dosage form is any one of seed treatment dry powder, seed treatment dispersible powder, seed treatment liquid, seed treatment emulsion, or seed treatment suspension; Preferably, the formulation dosage form is seed treatment suspension.

8. Use of the pesticide composition according to any one of claims 1 - 7 for controlling plant pests.

9. The use according to claim 8, characterized in that, The plants include corn, wheat, peanut, rice, and / or cotton; the pests are Agrotis ypsilon, white grubs, aphids, Ostrinia furnacalis, Chilo suppressalis, wireworms, thrips, and / or Athetis lepigone; Preferably, the plants are corn and / or peanut, and the pests are Agrotis ypsilon and / or white grubs.

10. The use according to claim 8, characterized in that, The pesticide composition is applied to plants, plant propagation materials, or subsequently growing plant organs, cultivation media, cultivation materials, or cultivation spaces at an effective dosage application rate.

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