Drug prepared from male tussah pupa as raw material and used for improving reproductive capacity
By using male tussus pupae with an effective accumulation temperature of 160-200℃ after diapause as raw material, the problem of underutilization of male tussus pupae in the existing technology was solved, and the reproductive capacity was significantly improved and cost reduction was achieved.
Patent Information
- Application Number
- CN202510713767.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-30
- Publication Date
- 2025-07-29
AI Technical Summary
In the prior art, no drugs are used to prepare reproductive capacity to improve reproductive capacity by using male tussus pupae, and the testosterone content of male tussus pupae has been fully utilized, resulting in high drug production costs.
Male tussus pupae with an effective accumulation temperature of 160-200℃ after diapause is removed as raw material to prepare male tussus pupae powder. Through crushing, filtration and low-temperature freeze-drying treatment, drugs to improve reproductive capacity are prepared.
It significantly improves the reproductive ability effect of the drug, promotes the number of captures and ejaculation times in mice, shortens the latency of ejaculation, and reduces the cost of drug production.
Smart Images
Figure CN120381467A_ABST
Abstract
Description
Technical Field
[0001] The present invention belongs to the field of deep processing of agricultural products, and particularly relates to a drug for improving reproductive ability prepared from male tussah pupae as raw materials. Background Art
[0002] Tussah is an insect that spins silk cocoons, belonging to the order Lepidoptera and the family Saturniidae. Its life undergoes four metamorphic stages: egg, larva, pupa, and adult (moth). Among them, the stage from pupa to adult (moth) is that after the tussah pupa breaks diapause, it begins to develop when the effective accumulated temperature reaches 10°C, and develops into a silkworm moth when the effective accumulated temperature reaches about 220°C. The male hormone testosterone in insects mainly plays a role in regulating reproductive behavior, affecting the mating behavior and reproductive ability of insects. Therefore, it is generally believed that the male hormone - testosterone in male silkworm moths for reproduction is higher than that in male tussah pupae. For example, in Li Shizhen's Compendium of Materia Medica, male silkworm moths are called "divine insects and national treasures", and are said to have miraculous effects of tonifying the liver and kidney, strengthening yang, and anti-aging. Therefore, at present, tussah pupae are the only new resource food approved by the National Health Commission that can be managed as ordinary food among the four metamorphic stages of tussah, while male silkworm moths are used as health care drugs for tonifying the kidney and strengthening yang, such as drugs like Meiling Pills and wild silkworm moths that have been developed. For this reason, the price of male tussah moths sold on the market is significantly higher than that of male tussah pupae, basically three times the price of male tussah pupae.
[0003] So far, there has been no relevant report on preparing a drug for improving reproductive ability using male tussah pupae. Summary of the Invention
[0004] The present invention is to solve the above problems existing in the prior art, and provides a drug for improving reproductive ability prepared from male tussah pupae as raw materials.
[0005] The technical solution of the present invention is: a drug for improving reproductive ability prepared from male tussah pupae as raw materials, which is prepared entirely from male tussah pupae with an effective accumulated temperature of 160 - 200°C after breaking diapause.
[0006] Preferably, it is prepared entirely from male tussah pupae with an effective accumulated temperature of 180°C after breaking diapause.
[0007] Preferably, the male tussah pupae are broken and filtered to obtain a filtrate, and then the filtrate is freeze-dried at low temperature to obtain male tussah pupa powder.
[0008] The present invention discovers that the testosterone content in male Chinese oak silkworm pupae with an effective accumulated temperature of 160 - 200 °C for diapause termination is significantly higher than that in male Chinese oak silkworm moths. Therefore, 100% of male Chinese oak silkworm pupae with an effective accumulated temperature of 160 - 200 °C after diapause termination are used to replace male Chinese oak silkworm moths to prepare drugs for improving reproductive ability, significantly reducing the production cost of the drugs. The experimental results of animals (mice) show that compared with 100% male Chinese oak silkworm moth powder, the drugs prepared by the present invention can significantly promote the capture times and ejaculation times of mice, shorten the ejaculation latency period, and have a better effect on improving reproductive ability. Brief Description of the Drawings
[0009] Figure 1 It is a schematic diagram showing the relationship between the effective accumulated temperature and testosterone content during the development of male Chinese oak silkworm pupae. Detailed Embodiments
[0010] Select healthy male Chinese oak silkworm live pupae that have terminated diapause, control their development through effective accumulated temperature. When the effective accumulated temperature reaches 180 °C, centrifuge and crush them, filter to remove impurities such as pupal skins, and then prepare male Chinese oak silkworm pupa powder by freeze-drying the filtrate at low temperature.
[0011] Experiment: I. Experiment on the changes of different effective accumulated temperatures and testosterone content during the development of male Chinese oak silkworm pupae 1. Test method Put the male Chinese oak silkworm live pupae after diapause termination in an incubator. The temperature of the incubator is 15 °C on the 1st and 2nd days, and the space humidity is maintained at 65 - 80%. Starting from the 3rd day, the temperature of the incubator is 20 °C until the Chinese oak silkworm pupae develop to the 3rd day of the moth, that is, the effective accumulated temperature reaches 240 °C. Randomly take 6 Chinese oak silkworm pupae at effective accumulated temperatures of 0 °C, 20 °C, 40 °C, 60 °C, 80 °C, 100 °C, 120 °C, 140 °C, 160 °C, 180 °C, 200 °C respectively, and randomly take 6 Chinese oak silkworm moths at 220 °C and 240 °C respectively. Mix their tissues and measure their testosterone content according to the requirements of the detection kit.
[0012] 2. Test results The test results are as Figure 1 . From Figure 1 It can be seen that during the process from male Chinese oak silkworm pupae after diapause termination (effective accumulated temperature of 0 °C) to the formation of male Chinese oak silkworm moths (i.e., the effective accumulated temperature ranges from 0 - 240 °C), the content change of the active ingredient testosterone is relatively large. Among them, the testosterone content is the lowest at an effective accumulated temperature of 0 °C, which is 19.22 pmol / L. At an effective accumulated temperature of 180 °C, the testosterone content reaches the highest, which is 40.12 pmol / L. When the accumulated temperature reaches 220 °C, that is, when the male Chinese oak silkworm pupae emerge as male Chinese oak silkworm moths (the 1st day of emergence), the testosterone content decreases to 29.68 pmol / L. When the accumulated temperature reaches 240 °C (the 3rd day of emergence), its testosterone content continues to decrease. The highest testosterone content during the whole development process is 2.1 times that of the lowest.
[0013] II. Experiment on the Effects of Male Tussah Silkworm Pupa Powder on the Number of Captures, Ejaculation Latency, and Ejaculation Frequency in Mice 1. Experimental Method Male ICR mice were randomly divided into 3 groups, with 10 mice in each group, namely the blank control group, the 0.2 g / kg male tussah silkworm pupa powder group, and the 0.2 g / kg male tussah silkworm moth powder group. They were given intragastric administration once a day for 30 consecutive days. The blank control group was given pure water by intragastric administration. The male tussah silkworm pupa powder is the drug prepared in the embodiment of the present invention, and the male tussah silkworm moth powder is prepared by substituting male tussah silkworm moths for male tussah silkworm pupae according to the method of the embodiment of the present invention.
[0014] 52 h before the mating experiment, 10 μg / only of estradiol benzoate injection was subcutaneously injected into the back of female mice, and 0.5 mg / only of progesterone injection was injected again 5 h before the start of the experiment. The experiment was carried out between 19:00 and 22:00 at night, keeping the surrounding environment quiet, and using red dim lights to create an environment suitable for estrus. First, each male mouse was individually placed in a cage to adapt to the environment for 10 min, and then 1 estrus female mouse was placed in each cage. The number of captures of mice (the number of times male mice captured female mice), the ejaculation latency of mice (the time interval from placing the female mouse to the male mouse first licking the penis), and the ejaculation frequency of mice (the number of times male mice licked the penis) were observed within 30 min.
[0015] 2. Experimental Results 2.1 The experimental results of the number of captures of mice are shown in the table respectively.
[0016] Table 1 Effects of Different Test Substances on the Number of Captures of Mice (times)
[0017] The results showed that compared with the control group, within 30 min, the number of captures of mice in the 0.2 g / kg male tussah silkworm pupa group and the 0.2 g / kg male tussah silkworm moth group were 11.26 and 7.38 times respectively, both significantly increased, which were 2.37 and 1.55 times that of the control group respectively, and the effect of the male tussah silkworm pupa group was significantly better than that of the male tussah silkworm moth group.
[0018] 2.2 The experimental results of the ejaculation latency of mice are shown in Table 2.
[0019] Table 2 Effects of Different Test Substances on the Ejaculation Latency of Mice (s)
[0020] The results showed that compared with the control group, within 30 min, the ejaculation latency of mice in the 0.2 g / kg male tussah silkworm pupa group and the male tussah silkworm moth group were 508.56 and 641.75 seconds respectively, both significantly shortened, and the ejaculation latency of the male tussah silkworm pupa group was significantly less than that of the male tussah silkworm moth group.
[0021] The test results of the ejaculation times of mice are shown in Table 3.
[0022] Table 3 Effects of different test substances on the ejaculation times of mice (times)
[0023] The results showed that, compared with the control group, within 30 minutes, the ejaculation times of mice in the male Antheraea pernyi pupae group and the male Antheraea pernyi moths group at 0.2 g / kg were 10.15 and 6.63 times respectively, both significantly increased, which were 2.31 and 1.51 times that of the control group respectively, and the effect of the male Antheraea pernyi pupae group was significantly better than that of the male Antheraea pernyi moths group.
Claims
1. A drug for enhancing reproductive ability prepared from male tussah silkworm pupae as raw materials, characterized in that It is made entirely from male oak silkworm pupae with an effective accumulated temperature of 160 - 200 °C from the release of diapause.
2. The drug for enhancing reproductive ability prepared from male tussah silkworm pupae according to claim 1, characterized in that It is made entirely from male oak silkworm pupae with an effective accumulated temperature of 180 °C from the release of diapause.
3. The drug for enhancing reproductive ability prepared from male tussah pupae according to claim 1 or 2, characterized in that: It is male oak silkworm pupa powder obtained by crushing male oak silkworm pupae, filtering to obtain a filtrate, and then subjecting the filtrate to freeze-drying at low temperature.