A biological 3D printing and culture integrated equipment

Abstract

The present invention belongs to the field of biological 3D printing technology, and specifically is an integrated biological 3D printing and culture equipment, comprising: a body, the top of the inner wall of the body is slidably connected to a sealing plate, the inner wall of the body is evenly provided with guide rods, and the inner wall of the body close to the sealing plate is slidably connected to a baffle; a supporting mechanism, the supporting mechanism is installed at the bottom of the inner wall of the body, and the inner wall of the supporting mechanism is socketed with the outer wall of the guide rod; an injection mechanism, the injection mechanism is installed above the inner wall of the body. By providing the supporting mechanism, the present invention enables the clamping component to fix the culture dish on the central axis of the carrier plate, and enables the clamping component and the alignment component to adjust the position of the injection head, and then perform injection, so that printing and culture are completed in one body, avoiding the need to manually move the culture dish after the injection is completed, which changes the environment of the cells and makes culture difficult, and at the same time ensures that the user can disassemble the injection mechanism for cleaning during the culture process.

Description

Technical Field

[0001] The present invention belongs to the field of biological 3D printing technology, and specifically relates to a biological 3D printing and culture integrated device. Background Art

[0002] 3D bioprinting technology is a new technology that uses specialized "bio-ink" to create artificial organs and biomedical products based on three-dimensional computer models. The core principle of 3D bioprinting is to layer "bio-ink" containing cells, growth factors, and nutrients under computer control to form three-dimensional tissue structures. This technology not only enables the in vitro generation of tissues or organs with specific biological functions, such as skin, cartilage, and blood vessels, but also has the potential to print more complex organs (such as the heart and kidneys) in the future.

[0003] In traditional biological tissue printing, printing and culture are mostly two independent parts. After printing, the cells are transferred to the corresponding culture environment. Due to the large difference between the environment in which they are printed and transferred and the culture environment, the culture environment requirements cannot be met, which reduces the printing quality and survival rate of the cells. Summary of the Invention

[0004] In view of the shortcomings of the existing technology, the present invention solves the technical problems by adopting the following technical solution: a biological 3D printing and culture integrated device, comprising:

[0005] A body, wherein a sealing plate is slidably connected to the top of the inner wall of the body, guide rods are evenly arranged on the inner wall of the body, and a baffle is slidably connected to the side of the inner wall of the body close to the sealing plate;

[0006] A supporting mechanism, the supporting mechanism being mounted on the bottom of the inner wall of the machine body, the inner wall of the supporting mechanism being sleeved with the outer wall of the guide rod;

[0007] An injection mechanism, the injection mechanism being installed above the inner wall of the machine body;

[0008] The injection mechanism comprises:

[0009] an alignment component, the alignment component being located above the supporting mechanism;

[0010] A disinfection component is installed on the outer wall of the alignment component and is used to clean and disinfect the bottom of the alignment component.

[0011] Furthermore, the injection mechanism further includes:

[0012] A slide rod, the slide rod being mounted behind the inner wall of the machine body, the outer wall of the slide rod being slidably connected to a mounting plate;

[0013] A slider, wherein the inner wall of the slider is slidably connected to the outer wall of the mounting plate.

[0014] Furthermore, the injection mechanism further comprises:

[0015] a second telescopic column, the second telescopic column being mounted on an outer wall of the slider, and an end of the second telescopic column away from the slider being connected to an outer wall of the alignment assembly;

[0016] The injection head is arranged inside the alignment component, and the outer wall of the injection head is clamped with the inner wall of the alignment component.

[0017] Furthermore, the supporting mechanism includes:

[0018] A lifting column, the bottom of which is mounted on the bottom of the inner wall of the machine body;

[0019] The bottom of the supporting plate is mounted on the top of the lifting column, and the top of the supporting plate is evenly provided with slideways.

[0020] Furthermore, the supporting mechanism further comprises:

[0021] A clamping assembly, the bottom of which is slidably connected to the top of the carrying plate, and three clamping assemblies are evenly arranged;

[0022] A culture dish, wherein the bottom of the culture dish contacts the top of the carrier plate, and the outer wall of the culture dish contacts the inner wall of the clamping assembly.

[0023] Furthermore, the clamping assembly includes:

[0024] A pressing block, the bottom of which is slidably connected to the top of the bearing plate, and a rotating member is rotatably mounted on the top of the inner wall of the pressing block;

[0025] a first telescopic column mounted on a top of the rotating member;

[0026] a first telescopic rod, the first telescopic rod being mounted on top of the first telescopic column;

[0027] An aiming ring is mounted on an end of the first telescopic rod away from the first telescopic column.

[0028] Furthermore, the alignment component includes:

[0029] A mounting frame, the mounting frame is arranged at an end of the second telescopic column away from the slider, and the inner wall of the mounting frame is clamped with the outer wall of the injection head;

[0030] a second telescopic rod, the second telescopic rod being disposed at the bottom of the mounting frame;

[0031] The receiving plate is installed at the bottom of the second telescopic rod, and the top of the receiving plate is rotatably connected to the bottom of the second telescopic rod.

[0032] Furthermore, the alignment component further includes:

[0033] A cleaning needle is installed on the top of the inner wall of the receiving tray. The top of the cleaning needle is connected to the inner wall of the bottom of the injection head. The cleaning needle is used to clear the bottom of the injection head. Initially, the cleaning needle and the receiving tray are away from the injection head.

[0034] A fixing rod, the fixing rod being arranged on a side of the bottom of the mounting frame away from the second telescopic rod;

[0035] The mounting ring is arranged at the bottom of the fixing rod, and the inner wall of the mounting ring is sleeved with the outer wall of the injection head.

[0036] Furthermore, the disinfection component includes:

[0037] A fixing frame, the top of which is arranged on the outer wall of the mounting frame;

[0038] The sleeve is mounted on the bottom of the fixed frame, and a strip groove is provided on the side of the sleeve so that the second telescopic rod can drive the receiving plate into the sleeve;

[0039] The foldable sleeve is installed on the top of the inner wall of the sleeve shell. The inner wall of the foldable sleeve is evenly provided with liquid outlet holes. The material of the foldable sleeve is a water-absorbing sponge material with resilience.

[0040] Furthermore, the disinfection mechanism further comprises:

[0041] The collar is symmetrically arranged on the outer wall of the folding sleeve, and the outer wall of the collar is clamped with the outer wall of the folding sleeve. The collar is a hose with small holes evenly opened inside, and the positions of the small holes are consistent with the positions of the liquid outlet holes;

[0042] The liquid outlet pipe is installed on the outer wall of the collar, the outer surface of the liquid outlet pipe is snap-connected with the inner wall of the sleeve, and the liquid outlet pipe is externally connected to the disinfectant so that the disinfectant can only flow out of the liquid outlet pipe in one direction.

[0043] The beneficial effects of the present invention are as follows:

[0044] 1. The present invention provides a supporting mechanism so that the clamping assembly fixes the culture dish on the central axis of the carrier plate, and the clamping assembly and the alignment assembly adjust the position of the injection head, and then the injection is performed. After the injection of the bio-ink is completed, the lifting column descends to move the culture dish away from the injection assembly, and the baffle blocks the space below the body, so that printing and culture are completed in the same body, avoiding the need to manually move the culture dish after the injection is completed, which changes the environment of the cells and makes culture difficult. At the same time, it ensures that the user can disassemble the injection mechanism for cleaning during the culture process to avoid affecting the cell culture environment.

[0045] 2. The present invention sets an injection mechanism, which will cause the injection to be paused during the injection process. At this time, the alignment component is close to the bottom of the injection head, blocking the bottom of the injection head to ensure the amount of injected ink, prevent excess bio-ink from dripping into the culture dish, and keep the injection head unobstructed. When the alignment component is away from the injection head and close to the disinfection component, the bottom of the alignment component is inserted into the disinfection component for cleaning and disinfection to avoid cells remaining on the surface of the alignment component, resulting in inconsistent cell activity before and after the printing process, affecting the print quality.

[0046] 3. The present invention provides an alignment component to avoid occasional dripping during the injection process due to the inability to immediately stop the extrusion of the bio-ink during ink control, which affects the printing accuracy. The outlet of the injection head is unblocked to maintain smooth liquid discharge and avoid blockage that affects the injection speed, thereby making it impossible to accurately control the flow rate of the bio-ink.

[0047] 4. The present invention provides a disinfection component so that after the foldable sleeve is squeezed, its inner wall contacts and squeezes the outer wall of the cleaning needle, thereby achieving cleaning; during the squeezing process, the disinfectant in the ring is squeezed out, so that the disinfectant is absorbed by the foldable sleeve, and the cleaning needle is wiped and soaked, so that the surface of the cleaning needle remains sterile, and the excess disinfectant is squeezed out of the foldable sleeve and flows along the cleaning needle into the receiving tray, so that the absorbent material in the receiving tray absorbs it, so that the cleaning needle always remains sterile during repeated use, avoiding changes in the properties before and after the injection of the biological ink, resulting in inconsistent printing accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0048] Figure 1 It is a structural schematic diagram of the present invention;

[0049] Figure 2 is a cross-sectional view of the present invention;

[0050] Figure 3 It is a structural schematic diagram of the supporting mechanism of the present invention;

[0051] Figure 4 It is a structural schematic diagram of the injection mechanism of the present invention;

[0052] Figure 5 It is a partial structural diagram of the injection mechanism of the present invention;

[0053] Figure 6 It is a structural schematic diagram of the clamping assembly of the present invention;

[0054] Figure 7 is a schematic structural diagram of the alignment assembly of the present invention;

[0055] Figure 8 It is a schematic structural diagram of the disinfection component of the present invention.

[0056] Figure: 1, body; 2, sealing plate; 3, supporting mechanism; 301, lifting column; 302, carrying plate; 303, slide; 304, clamping assembly; 3041, pressing block; 3042, rotating member; 3043, first telescopic column; 3044, first telescopic rod; 3045, aiming ring; 305, culture dish; 4, injection mechanism; 401, slide rod; 402, mounting plate; 403, slider; 404, alignment assembly Parts; 4041, mounting frame; 4042, second telescopic rod; 4043, receiving tray; 4044, cleaning needle; 4045, fixing rod; 4046, mounting ring; 405, disinfection assembly; 4051, fixing frame; 4052, sleeve; 4053, folding sleeve; 4054, liquid outlet; 4055, sleeve ring; 4056, liquid outlet tube; 406, second telescopic column; 407, injection head; 5, guide rod; 6, baffle. DETAILED DESCRIPTION

[0057] The present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for purposes of illustration and description and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments are chosen and described to better illustrate the principles of the invention and its practical application, and to enable those skilled in the art to understand the invention and design various embodiments with various modifications suitable for specific applications.

[0058] Example 1, please refer to Figure 1-Figure 5 The present invention provides a technical solution: a biological 3D printing and culture integrated equipment is described as follows.

[0059] include:

[0060] The body 1 has a sealing plate 2 slidably connected to the top of the inner wall of the body 1, and guide rods 5 are evenly arranged on the inner wall of the body 1. A baffle 6 is slidably connected to the side of the inner wall of the body 1 close to the sealing plate 2, and the width of the baffle 6 is less than the distance between the two guide rods 5;

[0061] The supporting mechanism 3 is mounted on the bottom of the inner wall of the body 1, and the inner wall of the supporting mechanism 3 is sleeved with the outer wall of the guide rod 5;

[0062] The injection mechanism 4 is installed above the inner wall of the body 1;

[0063] During operation, the injection mechanism 4 is loaded with bio-ink, the culture dish 305 is placed in the body 1, the supporting mechanism is aligned with the injection mechanism 4, the body 1 is turned on, and the cells inside the body 1 are placed in an environment with suitable temperature and humidity and stable conditions. Then the injection mechanism 4 starts to work, and the processed bio-ink is injected into the culture dish 305 as needed. Then the supporting mechanism 3 descends, away from the injection mechanism 4, and enters the bottom of the inner wall of the body 1. The user pushes the baffle 6 into the body 1 to separate the lower space inside the body 1 from the upper space, providing a stable environment for the cells.

[0064] The supporting mechanism 3 includes:

[0065] A lifting column 301, the bottom of which is mounted on the bottom of the inner wall of the body 1;

[0066] The bottom of the supporting plate 302 is mounted on the top of the lifting column 301 , and the top of the supporting plate 302 is evenly provided with slideways 303 .

[0067] The supporting mechanism 3 further includes:

[0068] A clamping assembly 304, the bottom of which is slidably connected to the top of the carrier plate 302, and three clamping assemblies 304 are evenly arranged;

[0069] The culture dish 305 has a bottom in contact with the top of the carrier plate 302 , and an outer wall of the culture dish 305 in contact with an inner wall of the clamping assembly 304 .

[0070] During operation, the lifting column 301 drives the supporting plate 302 to rise, so that the supporting plate 302 is flush with the bottom of the sealing plate 2, and then the user places the culture dish 305 in the middle of the clamping assembly 304, so that the clamping assembly 304 fixes the culture dish 305 at the central axis of the supporting plate 302, and adjusts the position of the injection head 407 with the clamping assembly 304 and the alignment assembly 404, and then performs the injection. After the injection of the bio-ink is completed, the lifting column 301 descends, so that the culture dish 305 is away from the injection assembly, and the baffle 6 blocks the space below the body 1, so that printing and culture are completed in one body 1, avoiding the need to manually move the culture dish 305 after the injection is completed, which changes the environment of the cells and makes it difficult to culture. At the same time, it ensures that the user can disassemble the injection mechanism for cleaning during the culture process to avoid affecting the culture environment of the cells.

[0071] The injection mechanism 4 includes:

[0072] Alignment assembly 404, alignment assembly 404 is located above the supporting mechanism 3;

[0073] Disinfection assembly 405 , which is installed on the outer wall of alignment assembly 404 , is used to clean and disinfect the bottom of alignment assembly 404 .

[0074] The injection mechanism 4 also includes:

[0075] Sliding rod 401, which is mounted behind the inner wall of the housing 1, and the outer wall of the sliding rod 401 is slidably connected to a mounting plate 402;

[0076] The slider 403 has an inner wall that is slidably connected to the outer wall of the mounting plate 402 .

[0077] The injection mechanism 4 also includes:

[0078] A second telescopic column 406 is mounted on the outer wall of the slider 403. The end of the second telescopic column 406 away from the slider 403 is connected to the outer wall of the alignment assembly 404. The slide bar 401, the slider 403 and the second telescopic rod 4042 work together to control the movement direction of the injection head 407.

[0079] The injection head 407 is disposed inside the alignment assembly 404 , and the outer wall of the injection head 407 is engaged with the inner wall of the alignment assembly 404 .

[0080] After the culture dish 305 is placed, the sliding rod 401 drives the mounting plate 402 to move, and the slider 403 and the second telescopic rod 4042 control the injection head 407 to approach the culture dish 305. After the culture dish 305 reaches the specified position, the alignment component 404 cooperates with the clamping component 304 to align the bottom of the injection head 407 with the central axis of the culture dish 305, and then the culture dish 305 is injected with biological ink. There will be a pause in the injection process. At this time, the alignment component 404 is close to the bottom of the injection head 407, blocking the bottom of the injection head 407 to ensure the amount of injected ink, prevent excess biological ink from dripping into the culture dish 305, and keep the injection head 407 unobstructed. When the alignment component 404 is away from the injection head 407 and close to the disinfection component 405, the bottom of the alignment component 404 is inserted into the disinfection component 405 for cleaning and disinfection to prevent cells from remaining on the surface of the alignment component 404, causing inconsistent cell activity before and after printing, affecting the printing quality.

[0081] Example 2, please refer to Figures 1-8 The present invention provides a technical solution: Based on Example 1, the clamping assembly 304 includes:

[0082] The bottom of the pressing block 3041 is slidably connected to the top of the carrier plate 302. The edge of the pressing block 3041 contacts and fits the outer wall of the culture dish 305. The top of the inner wall of the pressing block 3041 is rotatably mounted with a rotating member 3042.

[0083] A first telescopic column 3043 , which is mounted on top of the rotating member 3042 ;

[0084] A first telescopic rod 3044 , which is mounted on top of the first telescopic column 3043 ;

[0085] The aiming ring 3045 is installed at one end of the first telescopic rod 3044 away from the first telescopic column 3043. A laser sensor is provided on the top of the aiming ring 3045. The center of the aiming ring 3045 is placed in the same position as the axis of the culture dish 305 through the first telescopic rod 3044, so as to cooperate with the alignment component 404 to determine the position of the injection head 407.

[0086] After the culture dish 305 is placed on the supporting plate 302, the pressing block 3041 slides along the slide 303, and after contacting the culture dish 305, the culture dish 305 is located at the central axis of the supporting plate 302, so that the position of the culture dish 305 is determined and the culture dish 305 is kept stable. Then the rotating block rotates to make the first telescopic column 3043 in a vertical state, and the length of the first telescopic rod 3044 is adjusted to make the aiming ring 3045 in the same position as the axis of the culture dish 305. Then, with the cooperation of the alignment component 404, the position of the injection head 407 is determined, so that the injection head 407 is located at the center of the culture dish 305 for injection, so as to avoid inaccurate relative position between the print head and the culture dish 305, which makes it impossible to accurately deposit the printing material.

[0087] The alignment component 404 includes:

[0088] A mounting frame 4041 is provided at an end of the second telescopic column 406 away from the slider 403 , and the inner wall of the mounting frame 4041 is engaged with the outer wall of the injection head 407 ;

[0089] A second telescopic rod 4042 , which is disposed at the bottom of the mounting frame 4041 ;

[0090] The receiving tray 4043 is installed at the bottom of the second telescopic rod 4042. The top of the receiving tray 4043 is rotatably connected to the bottom of the second telescopic rod 4042. The receiving tray 4043 is filled with a material with good water absorption.

[0091] The alignment component 404 also includes:

[0092] A cleaning needle 4044 is mounted on the top of the inner wall of the receiving tray 4043. The top of the cleaning needle 4044 is engaged with the inner wall of the bottom of the injection head 407. The cleaning needle 4044 is used to clear and clean the bottom of the injection head 407. Initially, the cleaning needle 4044 and the receiving tray 4043 are located away from the injection head 407.

[0093] A fixing rod 4045 is provided at a side of the bottom of the mounting frame 4041 away from the second telescopic rod 4042;

[0094] Mounting ring 4046 is provided at the bottom of fixing rod 4045. The inner wall of mounting ring 4046 is engaged with the outer wall of injection head 407. A photosensor is provided at the bottom of mounting ring 4046. When the laser light emitted by the laser sensor is received by the photosensor, multiple groups of photosensors are matched with the laser sensors one by one, thereby determining whether the axis of mounting ring 4046 and aiming ring 3045 coincide with each other, and further determining whether the injection head 407 and culture dish 305 are on the same axis before injection is performed;

[0095] During the injection process, when the injection is paused, the second telescopic rod 4042 drives the receiving plate 4043 to rotate, so that the cleaning needle 4044 is located directly below the injection head 407. Driven by the second telescopic rod 4042, the cleaning needle 4044 is inserted into the injection head 407 to block the injection head 407 and also to dredge the injection head 407, thereby preventing slow liquid discharge and insufficient ink output, and then the injection is performed again.

[0096] To avoid pauses during the injection process, since the control of ink discharge cannot stop the extrusion of the bio-ink immediately, dripping may occasionally occur, which affects the printing accuracy. The outlet of the injection head 407 is unblocked to maintain smooth liquid discharge to avoid blockage that affects the injection speed, thereby making it impossible to accurately control the flow of the bio-ink.

[0097] The disinfection component 405 includes:

[0098] A fixing frame 4051, the top of which is arranged on the outer wall of the mounting frame 4041;

[0099] The housing 4052 is mounted on the bottom of the fixing frame 4051. A strip-shaped groove is provided on the side of the housing 4052 so that the second telescopic rod 4042 can drive the receiving plate 4043 into the housing 4052.

[0100] The foldable sleeve 4053 is installed on the top of the inner wall of the sleeve shell 4052. The inner wall of the foldable sleeve 4053 is evenly provided with liquid outlet holes 4054. The material of the foldable sleeve 4053 is a water-absorbing sponge material with resilience.

[0101] The disinfection component 405 also includes:

[0102] The collar 4055 is symmetrically arranged on the outer wall of the folding sleeve 4053. The outer wall of the collar 4055 is snap-fitted with the outer wall of the folding sleeve 4053. The collar 4055 is a hose with small holes evenly opened inside. The small holes are in the same position as the liquid outlet 4054.

[0103] The liquid outlet pipe 4056 is installed on the outer wall of the ring 4055. The outer surface of the liquid outlet pipe 4056 is snap-fitted to the inner wall of the sleeve 4052. The liquid outlet pipe 4056 is externally connected to the disinfectant so that the disinfectant can only flow out of the liquid outlet pipe 4056 in one direction.

[0104] After the cleaning needle 4044 is pulled out from the injection head 407, the receiving plate 4043 is rotated to a position where it coincides with the axis of the sleeve 4052 under the drive of the second telescopic rod 4042. Then, the receiving plate 4043 is inserted into the sleeve 4052 under the drive of the second telescopic rod 4042, so that the receiving plate 4043 contacts the bottom of the foldable sleeve 4053. During the upward movement, the foldable sleeve 4053 is squeezed, so that after the foldable sleeve 4053 is squeezed, its inner wall contacts and squeezes the outer wall of the cleaning needle 4044, thereby achieving cleaning;

[0105] During the extrusion process, the disinfectant in the ring 4055 is squeezed out, so that the disinfectant is absorbed by the folding sleeve 4053, and the cleaning needle 4044 is wiped and soaked, so that the surface of the cleaning needle 4044 remains sterile. The excess disinfectant is squeezed out of the folding sleeve 4053 and flows along the cleaning needle 4044 into the receiving tray 4043, so that the absorbent material in the receiving tray 4043 absorbs it, so that the cleaning needle 4044 always remains sterile during repeated use, avoiding changes in the properties of the biological ink before and after injection, which leads to inconsistent printing accuracy.

[0106] The specific workflow is as follows:

[0107] During operation, the injection mechanism 4 is filled with bio-ink, the culture dish 305 is placed in the body 1, and the lifting column 301 drives the carrier plate 302 to rise, so that the carrier plate 302 is flush with the bottom of the sealing plate 2. Then, the user places the culture dish 305 in the middle of the clamping assembly 304, and the clamping assembly 304 fixes the culture dish 305 on the central axis of the carrier plate 302. The clamping assembly 304 and the alignment assembly 404 adjust the position of the injection head 407. After the culture dish 305 is placed, the sliding rod 401 drives the mounting plate 402 to move, and the slider 403 and the second telescopic rod 4042 control the injection head 407 to approach the culture dish 305.

[0108] After the culture dish 305 reaches the designated position, the alignment component 404 cooperates with the clamping component 304 to align the bottom of the injection head 407 with the central axis of the culture dish 305. Then, the culture dish 305 is injected with bio-ink. During the injection process, there will be a pause. At this time, the alignment component 404 is close to the bottom of the injection head 407, blocking the bottom of the injection head 407 to ensure the amount of injected ink, prevent excess bio-ink from dripping into the culture dish 305, and keep the injection head 407 unobstructed. When the alignment component 404 is away from the injection head 407 and close to the disinfection component 405, the bottom of the alignment component 404 is inserted into the disinfection component 405 for cleaning and disinfection.

[0109] After the injection of the bio-ink is completed, the lifting column 301 descends, so that the culture dish 305 is away from the injection assembly, and the baffle 6 blocks the space below the body 1, so that printing and culture are completed in one body 1, avoiding the need to manually move the culture dish 305 after the injection is completed, which changes the environment of the cells and makes culture difficult. At the same time, it ensures that the user can disassemble the injection mechanism for cleaning during the culture process to avoid affecting the culture environment of the cells.

[0110] Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without making creative efforts should fall within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described and explained in the present invention shall be implemented in accordance with conventional means in the field unless otherwise specified or limited.

Claims

1. A biological 3D printing and culture integrated device, comprising: The machine body (1) is characterized in that: a sealing plate (2) is slidably connected to the top of the inner wall of the machine body (1), guide rods (5) are evenly arranged on the inner wall of the machine body (1), and a baffle (6) is slidably connected to the side of the inner wall of the machine body (1) close to the sealing plate (2); A supporting mechanism (3), the supporting mechanism (3) being mounted on the bottom of the inner wall of the machine body (1), the inner wall of the supporting mechanism (3) being sleeved with the outer wall of the guide rod (5); An injection mechanism (4), the injection mechanism (4) being installed above the inner wall of the body (1); The injection mechanism (4) comprises: an alignment component (404), the alignment component (404) being located above the supporting mechanism (3); A disinfection assembly (405), the disinfection assembly (405) being mounted on the outer wall of the alignment assembly (404), the disinfection assembly (405) being used to clean and disinfect the bottom of the alignment assembly (404); The injection mechanism (4) further comprises: A slide bar (401), the slide bar (401) being mounted behind the inner wall of the body (1), and the outer wall of the slide bar (401) being slidably connected to a mounting plate (402); a slider (403), wherein the inner wall of the slider (403) is slidably connected to the outer wall of the mounting plate (402); The injection mechanism (4) further comprises: a second telescopic column (406), the second telescopic column (406) being mounted on the outer wall of the slider (403), and an end of the second telescopic column (406) away from the slider (403) being connected to the outer wall of the alignment assembly (404); An injection head (407), the injection head (407) being arranged inside the alignment component (404), the outer wall of the injection head (407) being engaged with the inner wall of the alignment component (404); The supporting mechanism (3) comprises: A lifting column (301), the bottom of which is mounted on the bottom of the inner wall of the machine body (1); A bearing plate (302), the bottom of the bearing plate (302) is mounted on the top of the lifting column (301), and a slideway (303) is evenly provided on the top of the bearing plate (302); The supporting mechanism (3) further comprises: A clamping assembly (304), wherein the bottom of the clamping assembly (304) is slidably connected to the top of the carrier plate (302), and three clamping assemblies (304) are evenly arranged; a culture dish (305), wherein the bottom of the culture dish (305) contacts the top of the carrier plate (302), and the outer wall of the culture dish (305) contacts the inner wall of the clamping assembly (304); The alignment component (404) includes: A mounting frame (4041), the mounting frame (4041) being arranged at one end of the second telescopic column (406) away from the slider (403), the inner wall of the mounting frame (4041) being engaged with the outer wall of the injection head (407); a second telescopic rod (4042), the second telescopic rod (4042) being arranged at the bottom of the mounting frame (4041); A receiving plate (4043), the receiving plate (4043) is mounted on the bottom of the second telescopic rod (4042), and the top of the receiving plate (4043) is rotatably connected to the bottom of the second telescopic rod (4042); The alignment component (404) further includes: A cleaning needle (4044), the cleaning needle (4044) is mounted on the top of the inner wall of the receiving plate (4043), the top of the cleaning needle (4044) is sleeved with the inner wall of the bottom of the injection head (407), and the cleaning needle (4044) is used to clear and clean the bottom of the injection head (407); a fixing rod (4045), the fixing rod (4045) being arranged on a side of the bottom of the mounting frame (4041) away from the second telescopic rod (4042); A mounting ring (4046) is provided at the bottom of the fixing rod (4045), and the inner wall of the mounting ring (4046) is sleeved with the outer wall of the injection head (407).

2. The biological 3D printing and culture integrated device according to claim 1, characterized in that: The clamping assembly (304) includes: A pressing block (3041), the bottom of the pressing block (3041) is slidably connected to the top of the bearing plate (302), and a rotating member (3042) is rotatably mounted on the top of the inner wall of the pressing block (3041); a first telescopic column (3043), the first telescopic column (3043) being mounted on the top of the rotating member (3042); a first telescopic rod (3044), the first telescopic rod (3044) being mounted on the top of the first telescopic column (3043); An aiming ring (3045) is mounted on an end of the first telescopic rod (3044) away from the first telescopic column (3043).

3. The biological 3D printing and culture integrated device according to claim 1, characterized in that: The disinfection component (405) includes: A fixing frame (4051), wherein the top of the fixing frame (4051) is arranged on the outer wall of the mounting frame (4041); A casing (4052), the casing (4052) being mounted on the bottom of the fixing frame (4051); A foldable sleeve (4053) is installed on the top of the inner wall of the sleeve shell (4052), and the inner wall of the foldable sleeve (4053) is evenly provided with liquid outlet holes (4054).

4. The biological 3D printing and culture integrated device according to claim 3, characterized in that: The disinfection component (405) further comprises: A collar (4055), the collar (4055) being symmetrically arranged on the outer wall of the folding sleeve (4053), the outer wall of the collar (4055) being snap-connected with the outer wall of the folding sleeve (4053); A liquid outlet pipe (4056) is installed on the outer wall of the collar (4055), and the outer surface of the liquid outlet pipe (4056) is snap-connected with the inner wall of the sleeve (4052).

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