Phyllanthus emblica extract composition with function of reducing blood fat as well as preparation method and application of phyllanthus emblica extract composition

Abstract

The invention discloses an emblic leafflower fruit extract composition with a blood fat reducing function and a preparation method and application thereof, and the composition comprises the following components in parts by weight: 20-30 parts of an emblic leafflower fruit extract, 10-15 parts of a mixed extract, 5-10 parts of a houttuynia cordata stem extract, 10-20 parts of modified dietary fibers, 2-5 parts of haws, 4-8 parts of kudzu vine root powder and 0.1-0.3 part of beta-carotene. According to the composition containing the phyllanthus emblica extract, the phyllanthus emblica extract, the mulberry leaf extract, the pericarpium citri reticulatae extract and the herba houttuyniae extract are compounded, polysaccharide, phenols and flavonoid compounds in the extracts have the effect of reducing blood fat, and meanwhile, the modified dietary fibers are added, so that the blood fat reducing effect of the composition can be further improved; meanwhile, hydrogen bonds and interaction can be formed between the compound and phenolic compounds, the water solubility and bioaccessibility of the composition are improved, and by adding hawthorn and beta-carotene, the taste can be improved, and the blood fat reducing effect can be further improved.

Description

Technical Field

[0001] The present invention relates to the technical field of functional foods, and in particular to a phyllanthus emblica extract composition with blood lipid lowering function, and a preparation method and application thereof. Background Art

[0002] As people's living standards improve, their eating habits change, with an increased intake of high-calorie, high-fat foods, leading to a gradual rise in the incidence of hyperlipidemia. Dyslipidemia, commonly known as hyperlipidemia, is a chronic disease caused by impaired lipid metabolism that leads to high blood lipid levels. It can cause cardiovascular diseases such as atherosclerosis, coronary heart disease, and myocardial infarction. The "Dietary Guidelines for Adults with Hyperlipidemia (2023 Edition)" issued by my country's National Health Commission divides dyslipidemia into four types based on fasting venous serum test indicators: hypercholesterolemia (TC content ≥ 5.2 mmol / L); hypertriglyceridemia (TG content ≥ 1.7 mmol / L); low high-density lipoprotein cholesterol (HDL-C content < 1.0 mmol / L); and high low-density lipoprotein cholesterol (LDL-C content ≥ 3.4 mmol / L).

[0003] Generally speaking, lipid-lowering drugs are widely used in clinical practice, especially statins. While these drugs can effectively lower blood lipids, they also have side effects, particularly potential damage to liver function and muscles. In contrast, functional foods with lipid-lowering properties offer significant advantages in long-term lipid management.

[0004] Phyllanthus emblica is the dried and mature fruit of the plant Phyllanthus emblica in the Euphorbiaceae family. Various ancient herbal literature also records the various effects of Phyllanthus emblica. For example, "Newly Revised Materia Medica" records that Phyllanthus emblica is mainly used for treating wind deficiency and excessive heat. "Supplementary Records of Compendium of Materia Medica" records that Phyllanthus emblica is mainly used for tonifying and strengthening strength. "Shaoxing Materia Medica" records that eating the fruit of Phyllanthus emblica can relieve alcohol poisoning. "Compendium of Materia Medica" records that Phyllanthus emblica is cool in nature, sweet, sour and astringent in taste, and has the effects of clearing heat and cooling blood, promoting digestion and strengthening the stomach, promoting the production of body fluid and relieving cough. Research shows that Phyllanthus emblica is rich in bioactive substances such as phenols, vitamins, amino acids, and flavonoids. The results of modern pharmacological research also show that Phyllanthus emblica indeed has various pharmacological effects, such as having anti-microbial infection, anti-tumor, reducing blood lipid and blood sugar, lowering blood pressure, tonifying and other biological activities, and having no obvious toxic and side effects. For example, Chinese Patent (CN113786458A) discloses a pharmaceutical composition that can reduce blood lipid, reduce blood viscosity, and soften blood vessels, which includes Phyllanthus emblica, Lycium barbarum, Picrorhiza scrophulariiflora, Angelica sinensis, Pueraria lobata, Dracocephalum tanguticum, Coriandrum sativum, Lagotis glauca, Zhaxungao, Ligusticum wallichii, Meconopsis integrifolia, Astragalus membranaceus, Pterocephalus hookeri, Carthamus tinctorius, Dalbergia odorifera, Rubia cordifolia, Aristolochia moupinensis, Tea tree root, Butea monosperma, Dendrobium nobile, Arnebia euchroma, Saxifraga umbellata, Terminalia chebula, Hawthorn leaf, Terminalia bellirica, Polygonatum odoratum, Gastrodia elata, Herpetospermum pedunculosum, Aucklandia lappa, Ancistrocladus tectorius, Periploca sepium, Inula racemosa, Hippophae rhamnoides paste, Calculus bovis, Ligadu, Mulberry leaf. The raw materials in this patent are combined with traditional Chinese medicines and Tibetan medicines, which can have a good therapeutic and preventive effect on hyperlipidemia, with mild drug effects and low side effects. However, in this patent, multiple traditional Chinese medicines and Tibetan medicines are compounded, which is relatively complex to manufacture, and the effects of multiple drug components may interact with each other, resulting in an unpredictable blood lipid-lowering effect. Therefore, it is very important to develop safe and effective blood lipid-lowering compositions from food or raw materials homologous to medicine and food to improve the health level of the people. Summary of the Invention

[0005] The main object of the present invention is to propose a Phyllanthus emblica extract composition with blood lipid-lowering function, its preparation method and application, aiming to provide a composition developed from raw materials homologous to medicine and food, which can safely and effectively reduce blood lipid.

[0006] To achieve the above object, the present invention proposes a composition containing Phyllanthus emblica extract. Calculated by weight, the composition includes the following components: 20-30 parts of Phyllanthus emblica fruit extract, 10-15 parts of mixed extract, 5-10 parts of Houttuynia cordata stem extract, 10-20 parts of modified dietary fiber, 2-5 parts of hawthorn, 4-8 parts of kudzu root powder, and 0.1-0.3 parts of β-carotene.

[0007] Preferably, the particle size of the kudzu root powder is 40-120 mesh.

[0008] Preferably, the mixed extract includes the mixed extract of mulberry leaf, tangerine peel and Houttuynia cordata leaf, and the mass ratio of the mulberry leaf, tangerine peel and Houttuynia cordata leaf is (6-10):(2-4):(3-6).

[0009] Preferably, the preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of the Rosa roxburghii Tratt, wash, dry, crush it, sieve it to obtain Rosa roxburghii Tratt flesh powder, add deionized water, heat it to 60 - 80 °C, stir, centrifuge, and retain the supernatant. After washing the residue with deionized water, vacuum dry it, pulverize it, sieve it again, then add deionized water and cellulase, and enzymatically hydrolyze it in a water bath at 40 - 50 °C. After enzymatic hydrolysis, inactivate the enzyme, filter to obtain the supernatant. Combine the supernatants and concentrate them, then add guar gum solution and perform homogenization and shearing treatment, and then freeze-dry to obtain the modified dietary fiber.

[0010] Preferably, the sieve mesh for the first sieving is 50 - 100 meshes, and the sieve mesh for the second sieving is 200 - 300 meshes; The mass fraction of the cellulase is 0.2 - 0.4%; The dosage of the cellulase is 0.1 - 0.4% of the mass of the Rosa roxburghii Tratt; The dosage of the guar gum solution is 1 - 2% of the mass of the Rosa roxburghii Tratt; The rotation speed of the homogenization and shearing treatment is 8000 - 10000 rpm; The time of the homogenization and shearing treatment is 4 - 6 min; The temperature of the freeze-drying is -20 - -30 °C.

[0011] The present invention also provides a preparation method of a composition containing Phyllanthus emblica extract as described above, comprising the following steps: S1. Wash and pit fresh Phyllanthus emblica fruits, then break the pitted pulp and peel together with a blender, then perform microwave treatment, add an ethanol solution, stir evenly, then add glycine, heat and extract for a period of time, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; S2. Wash mulberry leaves, tangerine peels, and Houttuynia cordata Thunb. leaves respectively, then pulverize them, sieve them, mix them in proportion to obtain a mixture, add a complex enzyme, and perform enzymatic hydrolysis under ultrasonic conditions. After enzymatic hydrolysis, inactivate the enzyme, then add a methanol solution to the mixture for extraction, filter, add chitosan to the filtrate, stir evenly, then recover methanol and concentrate it under reduced pressure to obtain a mixed extract; S3. Wash Houttuynia cordata Thunb. stems and pulverize them, sieve them, add deionized water, stir, then add an ethanol solution and a citric acid solution, and extract under heating conditions, filter and evaporate and concentrate to obtain Houttuynia cordata Thunb. stem extract; S4. Wash the hawthorn, remove the pits, crush it, and sieve it to obtain hawthorn powder. Then mix the hawthorn powder, kudzu root powder, the Phyllanthus emblica fruit extract, the mixed extract, the Houttuynia cordata stem extract, and the modified dietary fiber in proportion, and add β-carotene in the corresponding proportion to obtain a composition containing Phyllanthus emblica extract.

[0012] Preferably, in step S1, the power of the microwave treatment is 550 - 650 W, and the time of the microwave treatment is 3 - 5 min; The temperature of the heating extraction is 50 - 60 °C, and the extraction time is 5 - 10 min.

[0013] Preferably, in step S1, the mass ratio of glycine to Phyllanthus emblica fruit is (1 - 3):(12 - 15).

[0014] Preferably, in step S2, the mass fraction of the methanol solution is 60 - 80%; The power of the ultrasonic wave is 500 - 600 W; The temperature of the extraction is 40 - 50 °C; The complex enzyme is a mixed enzyme of cellulase and pectinase; The mass ratio of chitosan to mulberry leaves is (1 - 3):(550 - 600).

[0015] Preferably, in step S3, the mass fraction of the ethanol solution is 60 - 80%; The mass fraction of the citric acid solution is 20 - 40%; The temperature of the heating is 50 - 70 °C.

[0016] The present invention further provides an application of the composition containing Phyllanthus emblica extract as described above in a functional food for reducing blood lipid.

[0017] Compared with the prior art, the present invention has the following beneficial effects: (1) The composition containing Phyllanthus emblica extract provided by the present invention is prepared by compounding Phyllanthus emblica extract, tangerine peel extract, mulberry leaf extract, and Houttuynia cordata extract. The polysaccharides, phenols, and flavonoid compounds in the extract have the effect of reducing blood lipid. At the same time, modified dietary fiber is added, which can not only further enhance its blood lipid-lowering effect, but also form hydrogen bonds with phenolic compounds and interact with each other to improve the water solubility and bioaccessibility of the composition. By adding hawthorn, not only can the taste be improved, but also the blood lipid-lowering effect can be further enhanced.

[0018] (2) In the process of extracting the composition containing Phyllanthus emblica extract provided by the present invention, different extraction processes are adopted according to the characteristics of each raw material, with high extraction efficiency. For the extraction of Phyllanthus emblica fruits, the plant cell walls are first broken by a wall breaker to increase the contact area between cells and solvents, thereby improving the extraction efficiency of active ingredients. Then, microwave-assisted solvent extraction is used to promote the faster release of components inside the cells. At the same time, glycine is added, which is equivalent to providing a hydrogen bond acceptor to form a strong hydrogen bond network with the hydrogen bond donors provided by phenols in the system, increasing the solubility of the entire reaction system and greatly enhancing the extraction efficiency. For the extraction of mulberry leaves, tangerine peel, and Houttuynia cordata leaves, since the main components of their cell walls are cellulose and pectin, the method of enzymatic hydrolysis-assisted hydrothermal solvent extraction can quickly break the cell walls, release more active ingredients, and improve the extraction efficiency. At the same time, adding natural polysaccharide chitosan can not only enhance the compatibility and biological activity of each component but also extend the storage period of the composition. For the extraction of Houttuynia cordata stems, adding organic acid citric acid to form an acidic environment and combining with the solvent extraction method can improve the active ingredients in Houttuynia cordata stems.

[0019] (3) The composition containing Phyllanthus emblica extract provided by the present invention can inhibit cholesterol synthesis and promote cholesterol metabolism, thereby reducing the total cholesterol level (TC) in the blood. At the same time, it can significantly reduce the concentrations of triglycerides (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly increase the concentration of high-density lipoprotein cholesterol (HDL-C), thus reducing blood lipids and treating hyperlipidemia. Moreover, the main components of the composition provided by the present invention - Phyllanthus emblica extract, mulberry leaf extract, tangerine peel extract, and Houttuynia cordata extract - are all derived from substances that are both medicine and food. The substances extracted by different methods are mixed in a specific ratio, with less irritation to the gastrointestinal mucosa. Even if consumed for a long time, it will not have significant side effects on consumers. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or in the prior art, the following will briefly introduce the drawings required for the description of the embodiments or the prior art. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other relevant drawings can also be obtained based on these drawings.

[0021] Figure 1 It is a schematic flow chart of an embodiment of the preparation method of the composition containing Phyllanthus emblica extract of the present invention.

[0022] The realization, functional characteristics, and advantages of the object of the present invention will be further described in conjunction with the embodiments and with reference to the drawings. DETAILED DESCRIPTION OF THE EMBODIMENTS

[0023] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be described clearly and completely below. For those not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. Reagents or instruments not specified by the manufacturer are all conventional products that can be obtained through commercial purchase. In addition, the technical solutions between various embodiments can be combined with each other, but it must be based on the ability of those of ordinary skill in the art to implement. When the combination of technical solutions conflicts with each other or cannot be implemented, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts belong to the scope of protection of the present invention.

[0024] The following further details the technical solutions of the present invention in conjunction with specific embodiments and drawings. It should be understood that the following embodiments are only used to explain the present invention and are not used to limit the present invention.

[0025] Example 1 A composition containing Phyllanthus emblica extract, comprising the following components: 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, 7 g of Houttuynia cordata stem extract, 15 g of modified dietary fiber, 3 g of hawthorn, 6 g of kudzu root powder, and 0.2 g of β-carotene.

[0026] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of Rosa roxburghii, weigh 150 g of Rosa roxburghii flesh, wash, dry, crush, and pass through a 60-mesh sieve to obtain Rosa roxburghii flesh powder. Add 2000 mL of deionized water, heat to 70 °C, stir, centrifuge for 30 min, and retain the supernatant. After washing the residue with deionized water twice, vacuum dry, crush, and pass through a 250-mesh sieve again. Then add 1000 mL of deionized water and 0.3 g of cellulase, and perform enzymatic hydrolysis in a water bath at 45 °C. After enzymatic hydrolysis, inactivate the enzyme, filter, and obtain the supernatant. Combine the supernatants and concentrate them. Then add 1.8 g of guar gum solution and perform homogenization and shearing treatment at 9000 rpm for 5 min. Then freeze-dry at -25 °C to obtain the modified dietary fiber.

[0027] The preparation method of the composition containing Phyllanthus emblica extract comprises the following steps: (1) Wash and pit 250 g of fresh Phyllanthus emblica fruits, then break the pitted pulp and peel together with a blender, then perform microwave treatment for 4 min, add an ethanol solution with a mass fraction of 60%, stir evenly, then add 20 g of glycine, heat to 55 °C and extract for 8 min, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; (2) Wash 60 g of mulberry leaves, 30 g of tangerine peel, and 50 g of houttuynia cordata leaves respectively, then crush them, pass through a 100-mesh sieve, mix them in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, carry out enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of 70% methanol solution to the mixture for extraction, filter, add 0.2 g of chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 70 g of houttuynia cordata stems, then crush them, pass through a 150-mesh sieve, add deionized water, stir, then add a 70% ethanol solution by mass fraction and a 30% citric acid solution by mass fraction, and carry out extraction under the condition of heating at 60 °C, filter and evaporate and concentrate to obtain an extract of houttuynia cordata stems; (4) Wash 8 g of hawthorn, remove the cores, then crush and pass through a sieve to obtain hawthorn powder. Then mix 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of phyllanthus emblica fruit extract, 12 g of mixed extract, 7 g of houttuynia cordata stem extract, and 15 g of modified dietary fiber, and add 0.2 g of β-carotene to obtain a composition containing phyllanthus emblica extract.

[0028] Example 2 A composition containing phyllanthus emblica extract, comprising the following components: 20 g of phyllanthus emblica fruit extract, 10 g of mixed extract, 5 g of houttuynia cordata stem extract, 10 g of modified dietary fiber, 2 g of hawthorn, 4 g of kudzu root powder, and 0.1 g of β-carotene.

[0029] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of the rosa roxburghii tratt, weigh 100 g of rosa roxburghii tratt flesh, wash, dry, and break it, pass through a 50-mesh sieve to obtain rosa roxburghii tratt flesh powder, add 1800 mL of deionized water, heat to 60 °C, stir, centrifuge for 30 min, retain the supernatant. After washing the residue with deionized water twice, vacuum dry, crush, and pass through a 200-mesh sieve again. Then add 800 mL of deionized water and 0.1 g of cellulase, carry out enzymatic hydrolysis in a water bath at 45 °C, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate, then add 1.5 g of guar gum solution, and carry out homogeneous shearing treatment at 8000 rpm for 4 min, and then freeze-dry at -20 °C to obtain modified dietary fiber.

[0030] The preparation method of the composition containing phyllanthus emblica extract comprises the following steps: (1) Wash 200 g of fresh phyllanthus emblica fruits, remove the cores, then break the de-stoned pulp and peel together with a blender, then microwave for 3 min, add a 70% ethanol solution by mass fraction, stir evenly, then add 15 g of glycine, heat to 50 °C and extract for 10 min, filter, and concentrate the filtrate to obtain phyllanthus emblica fruit extract; (2) Wash 50 g of mulberry leaves, 20 g of dried tangerine peels, and 30 g of houttuynia cordata leaves respectively, then crush them, pass through a 100-mesh sieve, mix them in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, carry out enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of 70% methanol solution to the mixture for extraction, filter, add 0.2 g of chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 50 g of houttuynia cordata stems, crush them, pass through a 100-mesh sieve, add deionized water, stir, then add an ethanol solution with a mass fraction of 60% and a citric acid solution with a mass fraction of 30%, carry out extraction under the condition of heating at 50 °C, filter and evaporate to concentrate to obtain an extract of houttuynia cordata stems; (4) Wash 5 g of hawthorns, remove the cores, crush them, and pass through a sieve to obtain hawthorn powder. Then mix 2 g of hawthorn powder, 4 g of kudzu root powder, 20 g of phyllanthus emblica fruit extract, 10 g of the mixed extract, 5 g of houttuynia cordata stem extract, and 10 g of modified dietary fiber, and add 0.1 g of β-carotene to obtain a composition containing phyllanthus emblica extract.

[0031] Example 3 A composition containing phyllanthus emblica extract, comprising the following components: 30 g of phyllanthus emblica fruit extract, 15 g of the mixed extract, 10 g of houttuynia cordata stem extract, 20 g of modified dietary fiber, 5 g of hawthorn, 5 g of kudzu root powder, 0.3 g of β-carotene.

[0032] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of the rosa roxburghii tratt, weigh 200 g of rosa roxburghii tratt flesh, wash, dry, and crush it, pass through a 100-mesh sieve to obtain rosa roxburghii tratt flesh powder, add 2200 mL of deionized water, heat to 80 °C, stir, centrifuge for 30 min, retain the supernatant. After washing the residue with deionized water 3 times, carry out vacuum drying, crush, and pass through a 300-mesh sieve again. Then add 1200 mL of deionized water and 0.4 g of cellulase, carry out enzymatic hydrolysis in a water bath at 45 °C, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate, then add 2 g of guar gum solution, and carry out homogenization and shearing treatment at 10000 rpm for 6 min, and then carry out freeze-drying at -30 °C to obtain the modified dietary fiber.

[0033] The preparation method of the composition containing phyllanthus emblica extract comprises the following steps: (1) Wash 300 g of fresh Phyllanthus emblica fruits, remove the cores, then break the de-stoned pulp and peel together with a blender, then perform microwave treatment for 5 min, add an ethanol solution with a mass fraction of 65%, stir evenly, then add 25 g of glycine, heat to 60 °C and extract for 5 min, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; (2) Wash 100 g of mulberry leaves, 40 g of dried tangerine peel, and 60 g of Houttuynia cordata leaves respectively, then pulverize them, pass through a 100-mesh sieve, mix them in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 0.8 g of pectinase, perform enzymatic hydrolysis under ultrasonic conditions of 600 W, inactivate the enzyme after enzymatic hydrolysis, then add 200 mL of 80% methanol solution to the mixture for extraction, filter, add 0.4 g of chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 100 g of Houttuynia cordata stems, pulverize them, pass through a 200-mesh sieve, add deionized water, stir, then add an ethanol solution with a mass fraction of 80% and a citric acid solution with a mass fraction of 30%, extract under the condition of heating at 70 °C, filter and evaporate and concentrate to obtain Houttuynia cordata stem extract; (4) Wash 10 g of hawthorn, remove the core and pulverize it, pass through a sieve to obtain hawthorn powder, then mix 5 g of hawthorn powder, 5 g of kudzu root powder, 30 g of Phyllanthus emblica fruit extract, 15 g of mixed extract, 10 g of Houttuynia cordata stem extract, and 20 g of modified dietary fiber, and add 0.3 g of β-carotene to obtain a composition containing Phyllanthus emblica extract.

[0034] Example 4 A composition containing Phyllanthus emblica extract, comprising the following components: 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, 7 g of Houttuynia cordata stem extract, 15 g of modified dietary fiber, 3 g of hawthorn, 6 g of kudzu root powder, and 0.2 g of β-carotene.

[0035] The preparation method of the modified dietary fiber includes the following steps: After removing the skin and seeds of Rosa roxburghii, weigh 150 g of Rosa roxburghii flesh, wash, dry, and break it, pass through a 60-mesh sieve to obtain Rosa roxburghii flesh powder, add 2000 mL of deionized water, heat to 70 °C, stir, centrifuge for 30 min, retain the supernatant, wash the residue with deionized water twice, vacuum dry, pulverize, and pass through a 250-mesh sieve again, then add 1000 mL of deionized water and 0.3 g of cellulase, perform enzymatic hydrolysis in a water bath at 45 °C, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate, then add 1.8 g of guar gum solution, and perform homogenization and shearing treatment at 9000 rpm for 5 min, then freeze-dry at -25 °C to obtain modified dietary fiber.

[0036] The preparation method of the composition containing Phyllanthus emblica extract includes the following steps: (1) Wash 250 g of fresh Phyllanthus emblica fruits, remove the cores, then break the de-stoned pulp and peel together with a blender, then microwave for 4 min, add an ethanol solution with a mass fraction of 60%, stir evenly, heat to 55 °C and extract for 8 min, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; (2) Wash 60 g of mulberry leaves, 30 g of tangerine peel, and 50 g of Houttuynia cordata leaves respectively, then pulverize, pass through a 100-mesh sieve, mix in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, carry out enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of a 70% methanol solution to the mixture for extraction, filter, and then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 70 g of Houttuynia cordata stems, pulverize, pass through a 150-mesh sieve, add deionized water, stir, then add an ethanol solution with a mass fraction of 70% and a citric acid solution with a mass fraction of 30%, extract under the condition of heating at 60 °C, filter and evaporate and concentrate to obtain Houttuynia cordata stem extract; (4) Wash 8 g of hawthorns, remove the cores and pulverize, pass through a sieve to obtain hawthorn powder, then mix 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, 7 g of Houttuynia cordata stem extract, and 15 g of modified dietary fiber, and add 0.2 g of β-carotene to obtain a composition containing Phyllanthus emblica extract.

[0037] Comparative Example 1 A composition containing Phyllanthus emblica extract, comprising the following components: 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, 7 g of Houttuynia cordata stem extract, 3 g of hawthorn, 6 g of kudzu root powder, 0.2 g of β-carotene.

[0038] The preparation method of the composition containing Phyllanthus emblica extract comprises the following steps: (1) Wash 250 g of fresh Phyllanthus emblica fruits, remove the cores, then break the de-stoned pulp and peel together with a blender, then microwave for 4 min, add an ethanol solution with a mass fraction of 60%, stir evenly, then add 20 g of glycine, heat to 55 °C and extract for 8 min, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; (2) Wash 60 g of mulberry leaves, 30 g of tangerine peel, and 50 g of Houttuynia cordata leaves respectively, then pulverize, pass through a 100-mesh sieve, mix in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, carry out enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of a 70% methanol solution to the mixture for extraction, filter, add 0.2 g of chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 70 g of Houttuynia cordata Thunb. stems, crush them, pass through a 150-mesh sieve, add deionized water, stir, then add a 70% ethanol solution and a 30% citric acid solution, and perform extraction under the condition of heating at 60 °C. Filter and evaporate to concentrate to obtain the Houttuynia cordata Thunb. stem extract; (4) Wash 8 g of hawthorn, remove the cores, crush them, and pass through a sieve to obtain hawthorn powder. Then mix 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, and 7 g of Houttuynia cordata Thunb. stem extract, and add 0.2 g of β-carotene to obtain a composition containing Phyllanthus emblica extract.

[0039] Comparative Example 2 A composition containing Phyllanthus emblica extract, comprising the following components: 25 g of Phyllanthus emblica fruit extract, 7 g of Houttuynia cordata Thunb. stem extract, 15 g of modified dietary fiber, 3 g of hawthorn, 6 g of kudzu root powder, and 0.2 g of β-carotene.

[0040] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of Rosa roxburghii Tratt., weigh 150 g of Rosa roxburghii Tratt. flesh, wash, dry, and crush it, pass through a 60-mesh sieve to obtain Rosa roxburghii Tratt. flesh powder, add 2000 mL of deionized water, heat to 70 °C, stir, centrifuge for 30 min, and retain the supernatant. After washing the residue with deionized water twice, perform vacuum drying, crush, and pass through a 250-mesh sieve again. Then add 1000 mL of deionized water and 0.3 g of cellulase, perform enzymatic hydrolysis in a water bath at 45 °C, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate, then add 1.8 g of guar gum solution, and perform homogenization and shearing treatment at 9000 rpm for 5 min, and then perform freeze-drying at -25 °C to obtain the modified dietary fiber.

[0041] The preparation method of the composition containing Phyllanthus emblica extract comprises the following steps: (1) Wash 250 g of fresh Phyllanthus emblica fruits, remove the cores, then break the de-stoned pulp and peel together with a blender, then perform microwave treatment for 4 min, add a 60% ethanol solution, stir evenly, then add 20 g of glycine, heat to 55 °C, and perform extraction for 8 min. Filter, and concentrate the filtrate to obtain the Phyllanthus emblica fruit extract; (2) Wash 70 g of Houttuynia cordata Thunb. stems, crush them, pass through a 150-mesh sieve, add deionized water, stir, then add a 70% ethanol solution and a 30% citric acid solution, and perform extraction under the condition of heating at 60 °C. Filter and evaporate to concentrate to obtain the Houttuynia cordata Thunb. stem extract; (3) Wash 8 g of hawthorn, remove the pits, then crush and sieve it to obtain hawthorn powder. Next, mix 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of Phyllanthus emblica fruit extract, 7 g of Houttuynia cordata stem extract, and 15 g of modified dietary fiber, and add 0.2 g of β-carotene to obtain a composition containing Phyllanthus emblica extract.

[0042] Comparative Example 3 A composition containing Phyllanthus emblica extract, comprising the following components: 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, 15 g of modified dietary fiber, 3 g of hawthorn, 6 g of kudzu root powder, and 0.2 g of β-carotene.

[0043] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and pits of Rosa roxburghii tratt, weigh 150 g of Rosa roxburghii tratt flesh, wash, dry, and crush it, sieve it through a 60-mesh sieve to obtain Rosa roxburghii tratt flesh powder. Add 2000 mL of deionized water, heat to 70 °C, stir, centrifuge for 30 min, and retain the supernatant. After washing the residue with deionized water twice, vacuum dry, crush, and sieve it through a 250-mesh sieve again. Then add 1000 mL of deionized water and 0.3 g of cellulase, heat in a water bath to 45 °C for enzymatic hydrolysis, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate them, then add 1.8 g of guar gum solution, and perform homogenization and shearing treatment at 9000 rpm for 5 min, and then freeze-dry at -25 °C to obtain the modified dietary fiber.

[0044] The preparation method of the composition containing Phyllanthus emblica extract comprises the following steps: (1) Wash 250 g of fresh Phyllanthus emblica fruits, remove the pits, then break the pitted pulp and peel together with a blender, then microwave for 4 min, add an ethanol solution with a mass fraction of 60%, stir evenly, then add 20 g of glycine, heat to 55 °C and extract for 8 min, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; (2) Wash 60 g of mulberry leaves, 30 g of tangerine peels, and 50 g of Houttuynia cordata leaves respectively, then crush and sieve them through a 100-mesh sieve, mix them in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, perform enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of 70% methanol solution to the mixture for extraction, filter, add 0.2 g of chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (3) Wash 8 g of hawthorn, remove the pits, then crush and sieve it to obtain hawthorn powder. Next, mix 3 g of hawthorn powder, 6 g of kudzu root powder, 25 g of Phyllanthus emblica fruit extract, 12 g of mixed extract, and 15 g of modified dietary fiber, and add 0.2 g of β-carotene to obtain a composition containing Phyllanthus emblica extract.

[0045] Comparative Example 4 A composition comprising the following components: 12 g of mixed extract, 7 g of houttuynia stem extract, 15 g of modified dietary fiber, 3 g of hawthorn, 6 g of kudzu root powder, and 0.2 g of β-carotene.

[0046] The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of Rosa roxburghii tratt, weigh 150 g of Rosa roxburghii tratt flesh, wash, dry, and crush it, pass through a 60-mesh sieve to obtain Rosa roxburghii tratt flesh powder, add 2000 mL of deionized water, heat to 70 °C, stir, centrifuge for 30 min, retain the supernatant, wash the residue with deionized water twice, vacuum dry, pulverize, and pass through a 250-mesh sieve again. Then add 1000 mL of deionized water and 0.3 g of cellulase, perform enzymatic hydrolysis in a water bath at 45 °C, inactivate the enzyme after enzymatic hydrolysis, filter to obtain the supernatant, combine the supernatants and concentrate, then add 1.8 g of guar gum solution, and perform homogenization and shearing treatment at 9000 rpm for 5 min, and then freeze-dry at -25 °C to obtain the modified dietary fiber.

[0047] The preparation method of the composition comprises the following steps: (1) Wash 60 g of mulberry leaves, 30 g of dried tangerine peel, and 50 g of houttuynia leaves respectively, then pulverize them, pass through a 100-mesh sieve, mix them in proportion to obtain a mixture, add a composite enzyme of 1 g of cellulase and 1 g of pectinase, perform enzymatic hydrolysis under ultrasonic conditions of 550 W, inactivate the enzyme after enzymatic hydrolysis, then add 300 mL of 70% methanol solution to the mixture for extraction, filter, add 0.2 g of chitosan to the filtrate, then recover methanol and concentrate under reduced pressure to obtain a mixed extract; (2) Wash 70 g of houttuynia stems, pulverize them, pass through a 150-mesh sieve, add deionized water, stir, then add a 70% ethanol solution and a 30% citric acid solution, perform extraction under the condition of heating at 60 °C, filter and evaporate and concentrate to obtain houttuynia stem extract; (3) Wash 8 g of hawthorn, remove the pits and pulverize it, pass through a sieve to obtain hawthorn powder, then mix 3 g of hawthorn powder, 6 g of kudzu root powder, 12 g of mixed extract, 7 g of houttuynia stem extract, and 15 g of modified dietary fiber in proportion, and add 0.2 g of β-carotene to obtain the composition.

[0048] Test methods and results (1) Experimental materials Experimental animals: 110 SPF-grade healthy adult female Wistar rats, purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. Rat age: 50 days Body weight: (210 ± 15) g High-fat diet formula (by mass percentage): 68.2% of the ordinary basic diet for rats, 1% cholesterol, 0.8% sodium cholate, 15% white sugar, 5% egg yolk powder, 10% lard.

[0049] Experimental reagents: TC, TG, HDL-C, and LDL-C detection kits (all purchased from Shanghai Fusheng Industrial Co., Ltd.) Simvastatin (purchased from Beijing Wansheng Pharmaceutical Co., Ltd.) (2) Experimental methods Feeding conditions: Feed the rats with the basic diet under the test environment, maintain a normal day-night cycle, allow free drinking water, and adaptively feed for 1 week.

[0050] Control the room temperature at 25°C and keep the relative humidity at 50% - 70%.

[0051] Grouping and modeling: After 1 week of adaptive feeding, randomly divide 110 rats into 2 groups: Hyperlipidemia group: 100 rats, fed with a high-fat diet Normal blood lipid group: 10 rats, fed with a normal basic diet Both groups are allowed free access to food and water and are fed for 3 weeks.

[0052] Blood collection and biochemical detection: After 3 weeks of modeling, fast the rats for 12 hours after the modeling is completed, collect blood samples through the tail vein, and collect serum samples.

[0053] Use TC, TG, HDL-C, and LDL-C kits to test the blood lipid contents in the serum.

[0054] The four blood lipid contents of the rats fed with the high-fat diet are significantly higher than those of the rats in the normal group, indicating that the modeling is successful and is used for the experiment.

[0055] Experimental grouping: Select 100 hyperlipidemic rats with blood lipid levels significantly higher than those of the normal group, randomly divide them into 10 groups, with 10 rats in each group, namely: High-fat control group, positive control group, Example 1 group, Example 2 group, Example 3 group, Example 4 group, Comparative Example 1 group, Comparative Example 2 group, Comparative Example 3 group, Comparative Example 4 group.

[0056] Administration: The positive control group is gavaged with 5 mg / kg of simvastatin The Example 1 - 4 groups and the Comparative Example 1 - 4 groups are respectively gavaged with 0.5 mg / kg of the corresponding prepared composition The high-fat control group and the normal blood lipid group are gavaged with the same volume of distilled water Gavage once a day for 30 days Feeding: All groups were fed with basal diet.

[0057] (3)Experimental results During the experiment, the body weight was measured once a day, and the appetite, behavior, feces, hair and animal death were observed. After 14 days of gavage, the animals were fasted for 12 h and allowed free access to water. Blood was taken from the femoral artery, allowed to stand for 30 min, and centrifuged at 3000 r / min for 20 min to separate the serum. The serum total cholesterol (TC) value, triglyceride (TG) value, high-density lipoprotein cholesterol (HDL-C) value, and low-density lipoprotein cholesterol (LDL-C) value were measured. The results are shown in Table 1. The obtained data were statistically processed and expressed as mean ± standard deviation (x±s). The significance of the difference was determined by t-test. The P value was calculated as follows: comparison between the high-fat model group and the blank group, and comparison between each administration group and the high-fat model group. (P<0.01 indicates extremely significant difference, P<0.05 indicates significant difference).

[0058] Table 1 Statistical results of hypolipidemic experiment Note: Compared with the high-fat model group, * indicates P<0.05, ** indicates P<0.01 As can be seen from Table 1 above, compared with the positive control group using simvastatin, the effects of Examples 1-4 in reducing total cholesterol and triglyceride after using the composition provided by the present invention are close to those of the positive control group, and the effects of Comparative Examples 1-4 in reducing total cholesterol and triglyceride are quite different from those of the positive control group; compared with the high-fat control group, the compositions provided in Examples 1-4 of the present invention have a significant effect in reducing total cholesterol and triglyceride, and in terms of reducing total cholesterol, the effect of any example group is better than the sum of the effects of any two groups of comparative examples, indicating that the components such as Phyllanthus emblica fruit extract, mixed extract, Houttuynia cordata stem extract, and modified dietary fiber play a synergistic role and promote each other to enhance the hypolipidemic effect of the composition; compared with the normal blood lipid group, after continuous gavage with the composition of Example 1-4 groups for 14 days, the hyperlipidemia symptoms were significantly reduced, approaching the blood lipid level before modeling. The mortality rate of mice after continuous gavage with the composition prepared in Examples 1-4 was 0 within 14 days, indicating that the composition prepared by the present invention is safe and non-toxic.

[0059] In summary, the composition containing Phyllanthus emblica extract provided by the present invention can inhibit cholesterol synthesis and promote cholesterol metabolism, thereby reducing the total cholesterol level (TC) in the blood. At the same time, it can significantly reduce the concentrations of triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C), and significantly increase the concentration of high-density lipoprotein cholesterol (HDL-C), thereby reducing blood lipids and treating hyperlipidemia. Moreover, the main components of the composition provided by the present invention, namely Phyllanthus emblica extract, tangerine peel extract, and Houttuynia cordata extract, are all derived from substances that are both medicine and food, and have less irritation to the gastrointestinal mucosa. Even if consumed for a long time, it will not cause significant side effects to the consumers.

[0060] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. For those skilled in the art, the present invention can have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the patent protection scope of the present invention.

Claims

1. A Phyllanthus emblica extract composition with the function of reducing blood lipid, characterized in that, By weight parts, the composition comprises the following components: 20 - 30 parts of Phyllanthus emblica fruit extract, 10 - 15 parts of mixed extract, 5 - 10 parts of Houttuynia cordata stem extract, 10 - 20 parts of modified dietary fiber, 2 - 5 parts of hawthorn, 4 - 8 parts of kudzu root powder, 0.1 - 0.3 parts of β - carotene.

2. The composition according to claim 1, characterized in that, The mixed extract comprises the mixed extract of mulberry leaves, tangerine peel and Houttuynia cordata leaves, and the mass ratio of the mulberry leaves, tangerine peel and Houttuynia cordata leaves is (5 - 10):(2 - 4):(3 - 6).

3. The composition according to claim 1, wherein The preparation method of the modified dietary fiber comprises the following steps: After removing the skin and seeds of Rosa roxburghii, wash, dry, crush it, sieve it to obtain Rosa roxburghii flesh powder, add deionized water, heat to 60 - 80 °C, stir, centrifuge, and retain the supernatant. After washing the residue with deionized water, vacuum dry, crush, sieve again, then add deionized water and cellulase, and enzymatically hydrolyze at 40 - 50 °C in a water bath. After enzymatic hydrolysis, inactivate the enzyme, filter to obtain the supernatant, combine the supernatants and concentrate, then add guar gum solution, and perform homogenization and shearing treatment, and then freeze - dry to obtain the modified dietary fiber.

4. The composition according to claim 3, characterized in that, The sieve for the first sieving is 50 - 100 mesh, and the sieve for the second sieving is 200 - 300 mesh; The mass fraction of the cellulase is 0.2 - 0.4%; The dosage of the cellulase is 0.1 - 0.4% of the mass of Rosa roxburghii; The dosage of the guar gum solution is 1 - 2% of the mass of Rosa roxburghii; The rotation speed of the homogenization and shearing treatment is 8000 - 10000 rpm; The time of the homogenization and shearing treatment is 4 - 6 min; The temperature of the freeze - drying is - 20 - - 30 °C.

5. A method for preparing a Phyllanthus emblica extract composition with lipid-lowering function according to any one of claims 1 to 4, characterized in that, Comprises the following steps: S1. Wash and pit fresh Phyllanthus emblica fruits, then break the pitted pulp and peel together with a blender, then perform microwave treatment, add an ethanol solution, stir evenly, then add glycine, heat and extract for a period of time, filter, and concentrate the filtrate to obtain Phyllanthus emblica fruit extract; S2. Wash mulberry leaves, tangerine peel and Houttuynia cordata leaves respectively, then crush, sieve, mix them in proportion to obtain a mixture, add a complex enzyme, and perform enzymatic hydrolysis under ultrasonic conditions. After enzymatic hydrolysis, inactivate the enzyme, then add a methanol solution to the mixture for extraction, filter, add chitosan to the filtrate, stir evenly, then recover methanol and concentrate under reduced pressure to obtain the mixed extract; S3. Wash Houttuynia cordata stems, then crush, sieve, add deionized water, stir, then add an ethanol solution and a citric acid solution, and extract under heating conditions, filter and evaporate and concentrate to obtain Houttuynia cordata stem extract; S4. Wash and pit hawthorn, then crush and sieve to obtain hawthorn powder, then mix the hawthorn powder, kudzu root powder with the Phyllanthus emblica fruit extract, mixed extract, Houttuynia cordata stem extract, modified dietary fiber in proportion, and add β - carotene in the corresponding proportion to obtain the Phyllanthus emblica extract composition with lipid - lowering function.

6. The preparation method according to claim 5, characterized in that, In step S1, the power of the microwave treatment is 550 - 650 W, and the time of the microwave treatment is 3 - 5 min; The temperature of the heating extraction is 50 - 60 °C, and the extraction time is 5 - 10 min.

7. The preparation method according to claim 5, characterized in that, In step S1, the mass ratio of glycine to Phyllanthus emblica fruits is (1~3):(12~15).

8. The preparation method according to claim 5, characterized in that, In step S2, the mass fraction of the methanol solution is 60~80%; the power of the ultrasonic wave is 500~600W; the temperature of the extraction is 40~50°C; the complex enzyme is a mixed enzyme of cellulase and pectinase.

9. The preparation method according to claim 5, characterized in that, In step S3, the mass fraction of the ethanol solution is 60~80%; the mass fraction of the citric acid solution is 20~40%; the temperature of the heating is 50~70°C.

10. Use of a composition according to any one of claims 1 to 4 in a functional food for reducing blood lipids.

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