Method for cultivating high-quality calculus bovis by utilizing flora

Abstract

The invention discloses a method for cultivating high-quality calculus bovis by using flora, which comprises the following steps: step 1, selecting a proper cattle body to carry out restraint local anesthesia, and carrying out an operation on the right rib of the cattle body; step 2, taking out the gall bladder injection vaccine, putting back and sewing; 3, normally feeding the cow body after the hand operation, and taking out the cow body after a certain time to cultivate calculus bovis; in the second step, a bacterial seedling culture solution is prepared from escherichia coli, lactobacillus, bifidobacterium, citrobacter, proteusbacillus vulgaris, enterococcus, glucose and nitrate. In the third step, the feeding feed comprises Chinese herbal medicine feed, and in the cattle feeding process, data are measured. Compared with the prior art, the method disclosed by the invention has the advantages that the quality of turning cattle gallbladder yellow into yellow can be improved through the flora culture solution.

Description

Technical Field

[0001] The present invention relates to the technical field of bezoar cultivation, and specifically to a method for cultivating high-quality bezoar using bacteria groups. Background Art

[0002] Bezoar is a calculus in the gallbladder, bile duct or hepatic duct of Bos taurus domesticus or Bubalus bubalis. Due to its functions such as clearing heat and detoxifying, clearing the heart and resolving phlegm, opening the orifices and cooling the liver, calming endogenous wind and relieving swelling, strengthening the heart and promoting bile flow, it has become the main raw material for more than 600 kinds of traditional Chinese patent medicines such as Angong Niuhuang Pills and Liushen Pills. However, the natural occurrence rate of natural bezoar is only about one in a thousand, making natural bezoar extremely scarce and having no substitutes. Bovine body cultivation of bezoar is based on the principle of the formation of bezoar in the bovine body. Through surgical operation, a special plastic mesh foreign body is implanted into the gallbladder of a live cow and an artificially cultivated bezoar bacterial vaccine is injected. Due to the action of the bezoar bacterial vaccine, the physical and chemical properties of the bile are changed, resulting in bacterial infection and other factors, thus inducing the formation of bezoar (gallstone).

[0003] The information disclosed in this background art section is only intended to enhance the understanding of the overall background of the present invention, and should not be regarded as an admission or any form of suggestion that this information constitutes the prior art already known to those of ordinary skill in the art. Summary of the Invention

[0004] The technical problem to be solved by the present invention is to overcome the above technical defects and provide a method for cultivating high-quality bezoar using bacteria groups, which can improve the quality of bezoar formation in the bovine body through the bacteria group culture solution.

[0005] To solve the above problems, the technical solution of the present invention is a method for cultivating high-quality bezoar using bacteria groups, including the following steps:

[0006] Step 1: Select a suitable bovine body for fixation and local anesthesia, and perform surgery on the right rib part of the bovine body;

[0007] Step 2: Take out the gallbladder, inject the bacterial vaccine and suture it back;

[0008] Step 3: Feed the bovine body normally after the surgery, and take out the cultivated bezoar after a certain period of time;

[0009] In the second step, the bacterial vaccine culture solution contains the following components: Escherichia coli, Lactobacillus, Bifidobacterium, Citrobacter, Proteus, Enterococcus, glucose, nitrate;

[0010] The feed in the third step includes Chinese herbal medicine feed, and data is measured during the feeding process of the bovine body.

[0011] Further, in step one, the local anesthetic is chosen as shallow anesthesia with Jing Song Ling. For the rib surgery, a cut of 8 - 10 cm is made upward at the intersection of the third intercostal space from the bottom on the right side of the cow and the horizontal line of the shoulder joint. Two fingers are inserted into the abdomen to take out the gallbladder, which is then incised about 4 cm and a mesh rack is placed in it.

[0012] Further, in step two, the bacterial vaccine culture solution is prepared into solutions with dosages of 100 million bacteria / ml, 150 million bacteria / ml, and 200 million bacteria / ml. Before suturing the gallbladder back, a suitable nucleus is implanted. It is better to use an oval porous plastic box wrapped with nylon cloth or cotton cloth as the nucleus, and the size of the nucleus accounts for 2 / 3 of the gallbladder volume.

[0013] Further, in step three, the Chinese herbal medicine feed includes berberine, astragalus polysaccharide, and polydatin, and the mass ratio of berberine, astragalus polysaccharide, and polydatin is 3:5:2.

[0014] Further, in step three, the detection includes bile components, bile pH value, and viscosity measurement: Bile from the cow's gallbladder and bile in the bezoar generator before surgery and on the 3rd, 7th, 15th, 30th, and 60th days after surgery is aseptically collected for the determination of the contents of bilirubin, cholic acid, mucin, Ca 2 tMg 2 tMg, K*, and Na*, and the bile pH value and bile viscosity are measured; Serum bilirubin, alanine aminotransferase, aspartate aminotransferase, jaundice index, and thymol turbidity test of the implanted yellow cattle are measured on the 3rd, 7th, 15th, 30th, and 60th days before and after surgery respectively; The bezoar is collected, weighed, and the contents of bilirubin and cholic acid in the bezoar are measured respectively.

[0015] Further, in step three, after the bezoar is collected, the dried bezoar is rubbed by hand in a porcelain plate or on sulfuric acid paper, then put into a colored ground glass bottle and stored in a cool and dry place to prevent moisture and sunlight exposure.

[0016] Further, in step three, within 2 weeks after the operation, the cattle should avoid strenuous movement, and be protected from rain and contamination. Antibiotics are prohibited from being used on the operated cattle.

[0017] Further, the method for cultivating high-quality bezoar using bacteria groups is applied to in-vivo bezoar cultivation in cattle.

[0018] The advantages of the present invention compared with the existing technology are as follows:

[0019] 1. The bacterial vaccine culture medium in the present invention contains the following components: Escherichia coli, Lactobacillus, Bifidobacterium, Citrobacter, Proteus, Enterococcus, glucose, and nitrate. Among them, Lactobacillus and Bifidobacterium can improve the reabsorption rate of bile acids. The induction of the bacterial flora can cause the substance of bilirubin glucuronide in bile to form calcium bilirubinate precipitate, and can also cause free bile acid to precipitate. Inflammatory exudate can increase the acidity of bile, thereby destroying the colloidal balance of bile fluid, causing the components of bezoar in bile to precipitate. Detailed implementation manners

[0020] In order to make the content of the present invention easier to be clearly understood, the technical solutions in the embodiments of the present invention will be described clearly and completely below.

[0021] A method for cultivating high-quality bezoar using bacterial flora, comprising the following steps:

[0022] Step 1: Select a suitable bovine body for fixation and local anesthesia, and perform surgery on the right rib part of the bovine body; the local anesthetic is selected as shallow anesthesia with Jing Song Ling. The rib part surgery is specifically an 8-10 cm incision upward at the intersection of the third intercostal space from the bottom on the right side of the bovine and the horizontal line of the shoulder joint. Insert two fingers into the abdomen to take out the gallbladder, cut about 4 cm, and place a mesh rack.

[0023] Step 2: Take out the gallbladder, inject the bacterial vaccine and put it back for suture; the bacterial vaccine culture medium contains the following components: Escherichia coli, Lactobacillus, Bifidobacterium, Citrobacter, Proteus, Enterococcus, glucose, and nitrate; the bacterial vaccine culture medium is prepared into solutions with doses of 100 million bacteria / ml, 150 million bacteria / ml, and 200 million bacteria / ml. Before putting the gallbladder back for suture, implant a suitable nuclear body. It is better to use an oval porous plastic box wrapped with nylon cloth or cotton cloth as the nuclear body. The size of the nuclear body accounts for 2 / 3 of the gallbladder volume.

[0024] Step 3: Feed the bovine body normally after the surgery, and take out the cultivated bezoar after a certain period of time; the feed includes Chinese herbal medicine feed, and the Chinese herbal medicine feed includes berberine, astragalus polysaccharide, and polydatin. The mass ratio of berberine, astragalus polysaccharide, and polydatin is 3:5:2.

[0025] During the feeding process of the bovine body, data are measured, and the detection includes bile components, bile pH value, and viscosity measurement: Sterilely collect the bile of the bovine gallbladder before surgery and 3, 7, 15, 30, and 60 days after surgery, and the bile in the bezoar generator, and perform bilirubin, bile acid, mucin, Ca 2 tMg 2Determine the contents of +, K*, and Na*, measure the pH value and viscosity of bile; measure the serum bilirubin, alanine aminotransferase, aspartate aminotransferase, jaundice index, and thymol turbidity test of the implanted yellow cattle before surgery and on the 3rd, 7th, 15th, 30th, and 60th days after surgery; collect bezoar, weigh it, and determine the contents of bilirubin and cholic acid in the bezoar respectively;

[0026] After collecting the bezoar, rub the dried bezoar by hand in a porcelain plate or on sulfuric acid paper, put it into a colored ground glass bottle, and store it in a cool and dry place to prevent moisture and sunlight. Avoid strenuous exercise of the cattle body within 2 weeks after the operation, and prevent rain and contamination. Antibiotics are prohibited for the operated cattle body.

[0027] Example 1

[0028] Select a suitable cattle body for fixation and local anesthesia, and perform surgery on the right rib of the cattle body; select Jing Song Ling for light anesthesia as the local anesthetic. For the rib surgery, make an 8-10 cm incision upward at the intersection of the third intercostal space from the bottom on the right side of the cattle and the horizontal line of the shoulder joint. Cut through the skin, muscle, and peritoneum, insert two fingers into the abdomen to take out the gallbladder, avoid the gallbladder blood vessels and cut about 4 cm, place the wire mesh frame, perform secondary continuous suture (the second buried suture), inject the vaccine after flushing, and replace the gallbladder into the abdominal cavity. Then suture the peritoneum, muscle, and skin in sequence. The whole operation takes about 20 minutes. Remove the sutures one week after the operation, and the cattle can be used for work two weeks later; prepare the vaccine culture solution, which contains the following components: Escherichia coli, Lactobacillus, Bifidobacterium, Citrobacter, Proteus, Enterococcus, glucose, nitrate; prepare the vaccine culture solution into solutions with doses of 100 million bacteria / ml, 150 million bacteria / ml, and 200 million bacteria / ml, and implant a suitable nucleus before putting the gallbladder back and suturing. Use an oval porous plastic box wrapped with nylon cloth or cotton cloth as the nucleus, and the size of the nucleus accounts for 2 / 3 of the gallbladder volume.

[0029] Feed the operated cattle body normally, and take out the cultivated bezoar after a certain period of time; the feed includes Chinese herbal medicine feed, and the Chinese herbal medicine feed includes berberine, astragalus polysaccharide, and polydatin. The mass ratio of berberine, astragalus polysaccharide, and polydatin is 3:5:2.

[0030] During the feeding process of the cattle body, measure the data, and the detection includes bile components, bile pH value, and viscosity measurement: aseptically collect the bile of the cattle gallbladder and the bile in the bezoar generator before surgery and on the 3rd, 7th, 15th, 30th, and 60th days after surgery, and perform tests on bilirubin, cholic acid, mucin, Ca 2 tMg 2 Determine the contents of +, K*, and Na*, measure the pH value and viscosity of bile; measure the serum bilirubin, alanine aminotransferase, aspartate aminotransferase, jaundice index, and thymol turbidity test of the implanted yellow cattle before surgery and on the 3rd, 7th, 15th, 30th, and 60th days after surgery; collect bezoar, weigh it, and determine the contents of bilirubin and cholic acid in the bezoar respectively;

[0031] Operation for taking out bezoar: The same as the operation for implanting nucleus. Take out the grid attached with bezoar. First, perform decontamination. Rinse it with clean water or suck out the moisture and viscous bile inside and outside the grid with blotting paper or absorbent cotton, and then dry it. It can be dried in an electric drying oven at about 50 °C, or the decontaminated grid can be placed in a dry and dark place to dry naturally.

[0032] After collecting the bezoar, knead the dried bezoar by hand in a porcelain plate or on sulfuric acid paper, put it into a colored ground glass bottle, and store it in a cool and dry place to prevent moisture and sunlight. Avoid strenuous exercise of the bovine body within two weeks after the operation, and prevent rain and contamination. Antibiotics are prohibited for the operated bovine body.

[0033] Result analysis. The following table shows the liver function tests 2 months and 12 months after bezoar cultivation:

[0034]

[0035]

[0036] The following table shows the contents of the main components of cultivated bezoar:

[0037]

[0038]

[0039] The present invention and its implementation manners have been described above. Such description is not restrictive. Generally speaking, if those of ordinary skill in the art are inspired by it and design similar structural manners and embodiments without creative work without departing from the purpose of the present invention, they shall fall within the protection scope of the present invention.

Claims

1. A method for cultivating high-quality bezoar bovis using bacterial flora, characterized in that, It includes the following steps: Step 1: Select a suitable bovine body for fixation and local anesthesia, and perform surgery on the right rib part of the bovine body; Step 2: Take out the gallbladder, inject the vaccine and put it back for suture; Step 3: Feed the bovine body normally after the operation, and take out the cultivated calculus bovis after a certain period of time; In Step 2, the vaccine culture solution contains the following components: Escherichia coli, Lactobacillus, Bifidobacterium, Citrobacter, Proteus, Enterococcus, glucose, nitrate; In Step 3, the feed includes Chinese herbal medicine feed, and data is measured during the feeding process of the bovine body.

2. A method for cultivating high-quality bezoar using a bacterial community according to claim 1, characterized in that: In Step 1, the local anesthetic is selected as Jing Song Ling for light anesthesia. The rib surgery is specifically an 8-10 cm incision upward at the intersection of the third intercostal space from the bottom on the right side of the bovine and the horizontal line of the shoulder joint. Insert two fingers into the abdomen to take out the gallbladder, cut about 4 cm, and place a wire mesh frame.

3. A method for cultivating high-quality bezoar bovis using a bacterial flora according to claim 1, characterized in that: In Step 2, the vaccine culture solution is prepared into solutions with doses of 100 million bacteria / ml, 150 million bacteria / ml, and 200 million bacteria / ml. Before putting the gallbladder back for suture, implant a suitable nucleus. It is better to use an oval porous plastic box wrapped with nylon cloth or cotton cloth as the nucleus, and the size of the nucleus accounts for 2 / 3 of the gallbladder volume.

4. A method for cultivating high-quality bezoar bovis using bacterial flora according to claim 1, characterized in that: In Step 3, the Chinese herbal medicine feed includes berberine, astragalus polysaccharide, and polydatin, and the mass ratio of berberine, astragalus polysaccharide, and polydatin is 3:5:

2.

5. A method for cultivating high-quality bezoar bovis using a bacterial community according to claim 1, characterized in that: In Step 3, the detection includes bile components, bile pH value, and viscosity measurement: aseptically collect the bovine gallbladder bile before surgery and on the 3rd, 7th, 15th, 30th, and 60th days after surgery, as well as the bile in the bezoar generator, and measure the contents of bilirubin, cholic acid, mucin, Ca 2 tMg2+, K*, and Na*, measure the bile pH value and bile viscosity; measure the serum bilirubin, alanine aminotransferase, aspartate aminotransferase, jaundice index, and thymol turbidity test in the implanted yellow cattle on the 3rd, 7th, 15th, 30th, and 60th days before and after surgery respectively; collect the bezoar, weigh it, and measure the contents of bilirubin and cholic acid in the bezoar respectively.

6. A method for cultivating high-quality bezoar bovis using a bacterial flora according to claim 1, characterized in that: In Step 3, after the calculus bovis is collected, the dried calculus bovis is rubbed by hand in a porcelain plate or on sulfuric acid paper, put into a colored ground glass bottle, and stored in a cool and dry place to prevent moisture and sunlight exposure.

7. A method for cultivating high-quality bezoar bovis using bacterial flora according to claim 1, characterized in that: In Step 3, avoid strenuous movement of the bovine body within 2 weeks after the operation, and prevent rain and contamination. Antibiotics are prohibited for the operated bovine body.

8. A method for cultivating high-quality calculus bovis using bacteria groups according to claims 1-7, which is applied to the in-vivo cultivation of calculus bovis in bovine bodies.

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