An egg-derived protein peptide composition for promoting skin elasticity and anti-aging, and a preparation method and application thereof

Abstract

The application relates to the cosmetic technical field and discloses an egg-derived protein peptide composition for promoting skin elasticity and anti-aging as well as a preparation method and application thereof. The egg-derived protein peptide composition is characterized by comprising the following components in percentage by mass: 0.1-10 wt% of PPP peptide, 0.1-5 wt% of Fenghuangyi polypeptide, 0.00001 wt% of sialic acid glycopeptide, 2-10 wt% of a humectant, 0.1-5 wt% of a penetration enhancer, 0.1-5 wt% of a thickening agent, 2-8 wt% of an emulsifying agent, 0.1-0.3 wt% of disodium EDTA, 0.01-0.5 wt% of a preservative and the balance of water. The three egg-derived protein peptides play roles from inside to outside, can increase skin elasticity, gloss and thickness from two aspects of skin cells and extracellular matrix, promote skin metabolism and improve skin barrier from inside, can solve problems of skin relaxation and thinning caused by external factors from outside, the three protein peptides promote each other from multiple targets, have synergistic effect, act on the skin in all directions and have more advantages than single peptides. The three protein peptides can cooperate with each other from multiple levels and jointly promote skin elasticity, moisture and tightness.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of cosmetics, in particular to a chicken egg-derived protein peptide composition for promoting skin elasticity and anti-aging, and a preparation method and application thereof. BACKGROUND

[0002] Skin is a very complex human organ, and its biomechanical properties are the result of complex interactions of different layers of skin. The extracellular matrix such as collagen and elastic fibers in the dermis is the main substance for maintaining skin elasticity, and collagen enhances the tensile strength of the tissue and regulates cell adhesion, while elastic fibers maintain collagen fiber fluctuations, allowing the skin to have contractility; collagen and elastic fibers maintain the structure and function of the skin, ensuring that the skin is smooth and elastic. However, an increase in the activity of elastase and collagenase can lead to excessive degradation of elastin and collagen, causing the skin to lose hydration and elasticity; at the same time, the presence of excess free radicals in the body can cause various damages, and when the damage caused by free radicals accumulates beyond the body's self-repairing ability, it can cause changes in cell differentiation state or even loss, leading to loss of skin elasticity and thinning. In addition, free radicals can directly affect collagen and elastin in the skin structure, promoting changes in protein conformation and thus affecting the mechanical properties of the skin, causing the skin to become loose.

[0003] Eggs are a globally shared "whole food" that is rich in various bioactive substances and has high biological potency, and are an important dietary route for human nutritional supplementation. Egg yolk accounts for about 50% of the mass of an egg and has the most abundant nutrients, including proteins, phospholipids, egg yolk oil, cholesterol, carotenoids, vitamins, etc. Phosvitin is an important component of egg yolk and is rich in clusters of phosphorylated serine residues, making it the protein with the highest phosphorylation degree in nature. PPP peptide (phosvitin phosphopeptide) is produced by enzymatic preparation of phosvitin, and it also has clusters of phosphoserine. Studies have shown that PPP peptide can chelate mineral ions such as Ca 2+ , Fe 2+ , has antioxidant and anti-stress functions, and can play an important regulatory role in cell signaling; and because PPP peptide is composed of small peptides, its ability to bind metals with phosphoserine ligands is easier, and the high content of phosphoserine residues makes its ability to scavenge DPPH free radicals, reducing power (inhibiting lipid oxidation), and metal chelating ability superior to those of phosvitin and the recognized antioxidant casein phosphopeptide (CPP).

[0004] The fibrous membrane between the eggshell and egg white is the protective tissue of the egg, which is a traditional Chinese medicine "Phoenix Robe". About 90% of the components of the Phoenix Robe are endogenous proteins and polypeptides, mainly collagen, keratin, and complex proteins combined with mucopolysaccharides. The Phoenix Robe protein is rich in disulfide bonds, which causes the protein molecules to be highly cross-linked, stable in nature, poor in solubility and low in bioavailability, limiting its application. Phoenix Robe polypeptide is an active peptide prepared by enzymatic treatment of Phoenix Robe and special process. It is composed of a mixture of low molecular peptides with different numbers of peptide bonds, and small molecular peptides with a molecular weight below 1000 Da account for 90.9%, which are more easily absorbed by the human body and show more diverse biological functions. Phoenix Robe contains more lysine oxidase-like protein 2, which is involved in the formation of cross-linked products of matrix collagen and elastin, and is essential for the biogenesis of connective tissue. Phoenix Robe polypeptide not only has the effects of accelerating epithelial formation, promoting skin growth, antioxidant and anti-inflammatory, but also has antioxidant properties that macromolecular proteins do not have, and can directly participate in the elimination of oxidative free radicals in the body, inhibit the activity of oxidative enzymes in the body, and improve the functional activity of antioxidant enzymes.

[0005] Sialic acid is a general term for nine-carbon sialic acid acylates, which widely exists in cell membrane glycoprotein, glycolipid, and even RNA of organisms. As a component of complex sugar, sialic acid is embedded in the terminal outermost side of all cell surfaces and most vertebrate glycoprotein and glycolipid molecules, and plays a crucial role in the interaction between cells and cells and cells and microenvironment; the active hydroxyl group of sialic acid can provide active hydrogen to combine with superoxide anion free radicals (O2 - ) and hydroxyl radicals (-OH), and play a role in resisting free radical oxidation. In addition, the hydroxyl group can also complex with metal ions to reduce the generation of free radicals. Sialic acid glycopeptide (SGP) is a natural N double antenna type glycopeptide containing sialic acid oligosaccharide chain, which is composed of eleven sugars and six peptides, and is one of the main components in egg yolk. Sialic acid glycopeptide can interact with cell surface receptors (such as sialic acid receptors) to activate signal transduction pathways, and can also regulate the activity of enzymes, receptors and transcription factors, thereby regulating the expression of genes, proteins and metabolites, and plays an important role in cell signaling pathways and immune response processes.

[0006] At present, most of the amino acids in skin elasticity promoting cosmetics are absorbed by the skin as raw materials for synthesizing proteins, and their skin care effect is limited, and the nutritional effect is greater than other. In addition, the existing cosmetics usually only include a single protein peptide, and the target point is single, and the skin care effect is limited. The multiple protein peptides in eggs are closer to the composition of human amino acids, have multiple target points, are not only more easily absorbed, have high biological activity, good skin care effect, but also safe and healthy, and have high acceptance. Based on this, it is of great significance to develop protein peptide composition cosmetics for promoting skin elasticity and anti-aging from egg sources. SUMMARY

[0007] The application provides an egg-derived protein peptide composition for promoting skin elasticity and anti-aging, a preparation method and application thereof.

[0008] To achieve the above-mentioned purpose, the application adopts the following technical solutions.

[0009] The first aspect of the application provides an egg-derived protein peptide composition for promoting skin elasticity and anti-aging, which comprises, in mass percentage:

[0010] 0.1-10 wt% of PPP peptide, 0.1-5 wt% of phoenix garment polypeptide, 0.00001 wt% of sialic acid glycopeptide, 2-10 wt% of humectant, 0.1-5 wt% of penetration enhancer, 0.1-5 wt% of thickening agent, 2-8 wt% of emulsifier, 0.1-0.3 wt% of disodium EDTA, 0.01-0.5 wt% of preservative, and the balance being water.

[0011] Preferably, the egg-derived protein peptide composition comprises, in mass percentage:

[0012] 5-10 wt% of PPP peptide, 3-5 wt% of phoenix garment polypeptide, 0.00001 wt% of sialic acid glycopeptide, 2-10 wt% of humectant, 0.1-5 wt% of penetration enhancer, 0.1-5 wt% of thickening agent, 2-8 wt% of emulsifier, 0.1-0.3 wt% of disodium EDTA, 0.01-0.5 wt% of preservative, and the balance being water.

[0013] Preferably, the humectant is at least one of glycerol, propylene glycol, butylene glycol, sodium hyaluronate or panthenol.

[0014] Preferably, the penetration enhancer is at least one of isosorbide dimethyl ether, inositol or betaine.

[0015] Preferably, the thickening agent is acrylate / C10-30 alkyl acrylate crosspolymer.

[0016] Preferably, the emulsifier is polysorbate-20 or polyglyceryl-10 myristate.

[0017] Preferably, the preservative is phenoxyethanol or p-hydroxyacetophenone.

[0018] Preferably, the egg-derived protein peptide composition comprises, in mass percentage:

[0019] PPP peptide 10wt%, Fenghuangyi polypeptide 5wt%, sialic acid glycopeptide 0.00001wt%, glycerin 5wt%, panthenol 1wt%, sodium hyaluronate 0.1wt%, isosorbide dimethyl ether 2.2wt%, inositol 1.8wt%, acrylic acid (ester) / C10-30 alkyl acrylate cross-linked polymer 3wt%, polyglyceryl-10 myristate 6wt%, disodium EDTA 0.2wt%, phenoxyethanol 0.5wt%, and the balance is water.

[0020] In a second aspect of the present application, a preparation method of the egg-derived protein peptide composition for promoting skin elasticity and anti-aging is provided, comprising:

[0021] Disperse the thickening agent in a proper amount of deionized water, and stir until uniform to obtain solution A;

[0022] Add disodium EDTA into the remaining deionized water, and stir until uniform to obtain solution B;

[0023] Mix solution A and solution B until uniform to obtain solution C;

[0024] Add the penetration enhancer and emulsifier into solution C in sequence, and stir until uniform at 70-90°C to obtain solution D;

[0025] Cool solution D to room temperature, and add the humectant, PPP peptide, Fenghuangyi polypeptide, sialic acid glycopeptide, and preservative while stirring, and ultrasonic dispersion to obtain the egg-derived protein peptide composition for promoting skin elasticity and anti-aging.

[0026] In a third aspect of the present application, the above egg-derived protein peptide composition for promoting skin elasticity and anti-aging is applied in cosmetics.

[0027] Compared with the prior art, the present application has the following beneficial effects:

[0028] The three protein peptides in the present application can cooperate with each other from multiple levels to promote skin elasticity, tightness and smoothness. First, Fenghuangyi polypeptide and sialic acid glycopeptide promote skin cell adhesion, proliferation and migration, protect skin cells, regulate extracellular matrix metabolism, thereby improving cell vitality, maintaining stable connection between cells and extracellular matrix, making the skin have strong structural support, and effectively restoring the elasticity and luster of the skin. Second, Fenghuangyi polypeptide and sialic acid glycopeptide inhibit elastase activity and collagenase activity, reduce the degradation of extracellular matrix, and increase the synthesis of collagen, thereby maintaining skin tension and elasticity. In addition, PPP peptide and sialic acid glycopeptide can effectively scavenge free radicals, reduce DNA damage, cell aging and changes in protein structure, and at the same time, prevent the increase of MMPs (such as collagenase and elastase) caused by oxidative stress activation of intracellular signaling pathways (such as NF-κB and MAPK pathways), thereby ensuring the thickness and elasticity of the skin. In addition, since free radicals can trigger inflammatory reactions and further exacerbate oxidative damage to the skin, PPP peptide can effectively reduce the production of pro-inflammatory cytokine IL-8 secretion, and sialic acid glycopeptide can inhibit leukocyte adhesion during tissue inflammation, both of which can inhibit the inflammatory response process and maintain skin tension and elasticity. BRIEF DESCRIPTION OF DRAWINGS

[0029] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiment description will be briefly introduced. Obviously, the drawings in the following description are only some embodiments described in the present application, and those skilled in the art can obtain other drawings according to these drawings without creative labor.

[0030] Figure 1 The test results of the elastase inhibition test of the egg-derived protein peptide composition of the present application are shown in the following figure.

[0031] Figure 2 The test results of the collagen type III expression of the egg-derived protein peptide composition of the present application are shown in the following figure. DETAILED DESCRIPTION

[0032] The technical solutions in the embodiments of the present application will be described clearly and completely in the following description of the embodiments of the present application in combination with the drawings. Obviously, the described embodiments are part of the embodiments of the present application, rather than all the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0033] In the following description of the present embodiment, the terms "include", "contain", "have" and "contain" and the like are all open terms, that is, they mean to include but not limited to.

[0034] In the following description of the embodiments, the term "and / or" is used to describe the association relationship of associated objects, which means that there can be three relationships, for example, A and / or B, which can represent the cases of A existing alone, B existing alone and A and B existing simultaneously. Wherein A and B can be singular or plural. The character " / " generally represents an "or" relationship between the associated objects before and after it.

[0035] In the following description of the embodiments, the term "at least one" means one or more, and "multiple" means two or more. "At least one of the following (one)" or similar expressions means any combination of these items, including any combination of single (one) or multiple items. For example, "at least one of a, b or c", or "at least one of a, b and c", can represent a, b, c, a-b (i.e. a and b), a-c, b-c, or a-b-c, where a, b and c can be single or multiple.

[0036] The terms used in the embodiments of the present application are merely for the purpose of describing specific embodiments, and are not intended to limit the present application. The singular forms "a" and "the" used in the embodiments of the present application and the appended claims are also intended to include the plural forms, unless the context clearly indicates otherwise.

[0037] Those skilled in the art should understand that in the following description of the embodiments of the present application, the order of the serial numbers does not mean the order of execution, and some or all steps can be executed in parallel or in sequence, and the execution order of each process should be determined according to its function and inherent logic, and should not constitute any limitation on the implementation process of the embodiments of the present application.

[0038] Those skilled in the art should understand that the numerical ranges in the embodiments of the present application should be understood as each intermediate value between the upper limit and the lower limit of the range. Each smaller range between any stated value or stated range and any other stated value or intermediate value within the stated range is also included in the present application. The upper limit and the lower limit of these smaller ranges can be independently included or excluded from the range.

[0039] Unless otherwise specified, the technical / scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the present application belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can also be used in the implementation or testing of the present application. All documents mentioned in the specification are incorporated by reference to disclose and describe the methods and / or materials related to the documents. In the event of conflict between the content of the specification and any incorporated document, the content of the specification shall prevail.

[0040] In a first aspect, the present application provides a chicken egg-derived protein peptide composition for promoting skin elasticity and anti-aging, comprising, in mass percentage:

[0041] 0.1-10 wt% of PPP peptide, 0.1-5 wt% of phoenix shell polypeptide, 0.00001 wt% of sialic acid glycopeptide, 2-10 wt% of humectant, 0.1-5 wt% of penetration enhancer, 0.1-5 wt% of thickening agent, 2-8 wt% of emulsifier, 0.1-0.3 wt% of disodium EDTA, 0.01-0.5 wt% of preservative, and the balance being water.

[0042] In the present application, the PPP peptide, phoenix shell polypeptide and sialic acid glycopeptide are all derived from chicken eggs, which are mild and safe, have no skin irritation and side effects, and can safely and effectively improve the elasticity, softness and toughness of the skin.

[0043] Among them, the PPP peptide (phosvitin phosphopeptide) is obtained by enzymatic hydrolysis of phosvitin, which has antioxidant and anti-stress functions and can scavenge free radicals in the body.

[0044] The phoenix shell polypeptide is an active peptide prepared by enzymatic hydrolysis of chicken eggshell membrane, i.e. phoenix shell, which is mainly composed of small molecule peptides. It not only has the functions of accelerating epithelial formation, promoting skin growth, antioxidant and anti-inflammatory, but also has antioxidant properties that macromolecular proteins do not have, and can directly participate in the elimination of oxidative free radicals in the body, inhibit the activity of oxidative enzymes in the body, and enhance the functional activity of antioxidant enzymes.

[0045] Sialic acid glycopeptide (SGP) is a natural N-biantennary glycopeptide containing sialic acid oligosaccharide chains, which is one of the main components in chicken egg yolk. It can interact with cell surface receptors (such as sialic acid receptors), activate signal transduction pathways, and also regulate the activity of enzymes, receptors and transcription factors, thereby regulating the expression of genes, proteins and metabolites, and plays an important role in cell signaling pathways and immune response processes. It has anti-inflammatory, antioxidant and anticancer properties and is involved in various metabolic processes.

[0046] The present application combines the advantages of three peptides, and can improve skin firmness and structure from different mechanisms and levels, and promote skin elasticity and smoothness. Specifically, the three protein peptides in the present application can cooperate with each other from multiple levels to jointly promote skin elasticity, moisture and firmness. First, Fenghuangyi polypeptide and sialic acid glycopeptide promote skin cell adhesion, proliferation and migration, protect skin cells, and regulate extracellular matrix metabolism, thereby improving cell vitality, maintaining stable connection between cells and extracellular matrix, making the skin have a solid structural support, and effectively restoring the elasticity and luster of the skin; second, Fenghuangyi polypeptide and sialic acid glycopeptide inhibit elastase activity and collagenase activity, reduce the degradation of extracellular matrix, and increase the synthesis of collagen, thereby maintaining skin tension and elasticity; in addition, PPP peptide and sialic acid glycopeptide can effectively scavenge free radicals, reduce DNA damage, cell aging and changes in protein structure, and at the same time, prevent oxidative stress from activating the expression of MMPs (such as collagenase and elastase) caused by intracellular signaling pathways (such as NF-κB and MAPK pathways), thereby ensuring the thickness and elasticity of the skin. In addition, since free radicals can trigger inflammatory reactions, further exacerbating oxidative damage to the skin, PPP peptide can effectively reduce the production of pro-inflammatory cytokine IL-8 secretion, and sialic acid glycopeptide can inhibit leukocyte adhesion during tissue inflammation, both of which can inhibit the inflammatory response process, thereby maintaining skin tension and elasticity.

[0047] The present application can act from the inside out through three egg-derived protein peptides. From the inside, the present application can increase skin elasticity, luster and thickness from two levels of skin cells and extracellular matrix, promote skin metabolism, and improve skin barrier; from the outside, the present application can solve the problems of skin relaxation and thinning caused by external factors, and the three can promote each other from multiple targets, synergistically, and act on the skin from all directions, which is more advantageous than a single peptide.

[0048] As a preferred embodiment of the present application, the egg-derived protein peptide composition comprises, by mass percentage:

[0049] PPP peptide 5-10 wt%, Fenghuangyi polypeptide 3-5 wt%, sialic acid glycopeptide 0.00001 wt%, humectant 2-10 wt%, penetration enhancer 0.1-5 wt%, thickening agent 0.1-5 wt%, emulsifier 2-8 wt%, disodium EDTA 0.1-0.3 wt%, preservative 0.01-0.5 wt%, and the balance is water.

[0050] In the present application, the humectant includes at least one of glycerol, propylene glycol, butylene glycol, sodium hyaluronate or panthenol, and preferably includes a mixed humectant containing two or more, such as a mixture of glycerol, panthenol / propylene glycol and sodium hyaluronate, a mixture of glycerol, propylene glycol and butylene glycol.

[0051] In the present application, the penetration enhancer is at least one of isosorbide dimethyl ether, inositol or betaine; the thickening agent is acrylates / C10-30 alkyl acrylate crosspolymer; the emulsifier is polysorbate-20 or polyglyceryl-10 myristate; and the preservative is phenoxyethanol or p-hydroxyacetophenone.

[0052] As a preferred aspect of the present application, the egg-derived protein peptide composition comprises, in mass percentage:

[0053] PPP peptide 10wt%, phoenix clothing polypeptide 5wt%, sialic acid glycopeptide 0.00001wt%, glycerol 5wt%, panthenol 1wt%, sodium hyaluronate 0.1wt%, isosorbide dimethyl ether 2.2wt%, inositol 1.8wt%, acrylates / C10-30 alkyl acrylate crosspolymer 3wt%, polyglyceryl-10 myristate 6wt%, disodium EDTA 0.2wt%, phenoxyethanol 0.5wt%, and the balance is water.

[0054] In a second aspect, the present application provides a method for preparing an egg-derived protein peptide composition for promoting skin elasticity and anti-aging, comprising:

[0055] Disperse the thickening agent in a proper amount of deionized water, and stir until uniform to obtain solution A;

[0056] Add disodium EDTA to the remaining deionized water, and stir until uniform to obtain solution B;

[0057] Mix solution A and solution B until uniform to obtain solution C;

[0058] Add the penetration enhancer and the emulsifier to solution C in sequence, and stir until uniform at 70-90°C; preferably, the stirring rate is 100-200 rpm, and the stirring time is 20-30 min;

[0059] Cool solution D to room temperature, and add the humectant, the PPP peptide, the phoenix clothing polypeptide, the sialic acid glycopeptide and the preservative while stirring, and ultrasonic dispersion to obtain the egg-derived protein peptide composition for promoting skin elasticity and anti-aging. Preferably, the ultrasonic dispersion frequency is 40 kHz, the power is 200 W, and the ultrasonic dispersion time is 40-60 min.

[0060] The egg-derived protein peptide composition of the present application can comprehensively improve skin firmness and structure, and promote skin elasticity and smoothness. It can be used as a cosmetic product or for preparing a cosmetic product.

[0061] The present application is further illustrated by the following specific examples.

[0062] Example 1

[0063] Example 1's egg-derived protein peptide composition for promoting skin elasticity and anti-aging comprises, by weight percentage:

[0064] PPP peptide 10wt%, Phoenix skin polypeptide 5wt%, Sialic acid glycopeptide 0.00001wt%, Glycerin 5wt%, Panthenol 1wt%, Sodium hyaluronate 0.1wt%, Isosorbide dimethyl ether 2.2wt%, Inositol 1.8wt%, Acrylates / C10-30 Alkyl Acrylate Crosspolymer 3wt%, Polyglycerol-10 Myristate 6wt%, Disodium EDTA 0.2wt%, Phenoxyethanol 0.5wt%, Balance: Water.

[0065] Its preparation methods include:

[0066] Disperse the acrylate / C10-30 alkanol acrylate crosspolymer in an appropriate amount of deionized water and stir until homogeneous to obtain solution A;

[0067] Add disodium EDTA to the remaining deionized water and stir until homogeneous to obtain solution B;

[0068] Mix solutions A and B, stir well, and you will get solution C.

[0069] Isosorbide dimethyl ether, inositol, and polyglycerol-10 myristate were added to solution C in sequence, and the mixture was stirred at 100 rpm for 30 min at 85 °C to obtain solution D.

[0070] After solution D was cooled to room temperature, glycerol, panthenol, sodium hyaluronate, PPP peptide, phoenix skin polypeptide, sialic acid glycopeptide and phenoxyethanol were added sequentially while stirring. The mixture was then ultrasonically dispersed at a frequency of 40 kHz, a power of 200 W and a time of 50 min to obtain an egg-derived protein peptide composition that promotes skin elasticity and anti-aging.

[0071] Example 2

[0072] Example 2's egg-derived protein peptide composition for promoting skin elasticity and anti-aging comprises, by weight percentage:

[0073] PPP peptide 10wt%, phoenix skin polypeptide 0.1wt%, sialic acid glycopeptide 0.00001wt%, glycerol 8wt%, propylene glycol 1wt%, butylene glycol 1wt%, isosorbide dimethyl ether 2wt%, betaine 1wt%, acrylate / C10-30 alkanol acrylate crosspolymer 5wt%, polyglycerol-10 myristate ester 8wt%, disodium EDTA 0.3wt%, phenoxyethanol 0.1wt%, balance water.

[0074] Its preparation methods include:

[0075] Disperse the acrylate / C10-30 alkanol acrylate crosspolymer in an appropriate amount of deionized water and stir until homogeneous to obtain solution A;

[0076] Add disodium EDTA to the remaining deionized water and stir until homogeneous to obtain solution B;

[0077] Mix solutions A and B, stir well, and you will get solution C.

[0078] Isosorbide dimethyl ether, betaine, and polyglycerol-10 myristate were added to solution C in sequence, and stirred at 200 rpm for 20 min at 90 °C to obtain solution D.

[0079] After solution D was cooled to room temperature, glycerol, propylene glycol, butylene glycol, PPP peptide, phoenix skin polypeptide, sialic acid glycopeptide, and phenoxyethanol were added sequentially while stirring. The mixture was then ultrasonically dispersed at a frequency of 40 kHz, a power of 200 W, and a time of 40 min to obtain an egg-derived protein peptide composition that promotes skin elasticity and anti-aging.

[0080] Example 3

[0081] Example 3's egg-derived protein peptide composition for promoting skin elasticity and anti-aging comprises, by weight percentage:

[0082] PPP peptide 5wt%, phoenix skin polypeptide 3wt%, sialic acid glycopeptide 0.00001wt%, glycerol 4wt%, propylene glycol 4wt%, sodium hyaluronate 0.2wt%, isosorbide dimethyl ether 0.1wt%, acrylate / C10-30 alkanol acrylate crosspolymer 1wt%, polyglycerol-10 myristate ester 4wt%, disodium EDTA 0.25wt%, phenoxyethanol 0.3wt%, balance water.

[0083] Its preparation methods include:

[0084] Disperse the acrylate / C10-30 alkanol acrylate crosspolymer in an appropriate amount of deionized water and stir until homogeneous to obtain solution A;

[0085] Add disodium EDTA to the remaining deionized water and stir until homogeneous to obtain solution B;

[0086] Mix solutions A and B, stir well, and you will get solution C.

[0087] Isosorbide dimethyl ether and polyglycerol-10 myristate were added to solution C in sequence, and stirred at 120 rpm for 28 min at 80 °C to obtain solution D.

[0088] After solution D was cooled to room temperature, glycerol, propylene glycol, butylene glycol, PPP peptide, phoenix skin polypeptide, sialic acid glycopeptide, and phenoxyethanol were added sequentially while stirring. The mixture was then ultrasonically dispersed at a frequency of 40 kHz, a power of 200 W, and a time of 47 min to obtain an egg-derived protein peptide composition that promotes skin elasticity and anti-aging.

[0089] Example 4

[0090] Example 4's egg-derived protein peptide composition for promoting skin elasticity and anti-aging comprises, by weight percentage:

[0091] PPP peptide 0.1wt%, phoenix skin polypeptide 0.1wt%, sialic acid glycopeptide 0.00001wt%, glycerol 2wt%, isosorbide dimethyl ether 3wt%, inositol 1wt%, betaine 1wt%, acrylate / C10-30 alkanol acrylate crosspolymer 0.11wt%, polysorbate-20 2wt%, disodium EDTA 0.1wt%, phenoxyethanol 0.01wt%, balance water.

[0092] Its preparation methods include:

[0093] Disperse the acrylate / C10-30 alkanol acrylate crosspolymer in an appropriate amount of deionized water and stir until homogeneous to obtain solution A;

[0094] Add disodium EDTA to the remaining deionized water and stir until homogeneous to obtain solution B;

[0095] Mix solutions A and B, stir well, and you will get solution C.

[0096] Isosorbide dimethyl ether, inositol, betaine, and polysorbate-20 were added sequentially to solution C, and the mixture was stirred at 150 rpm for 25 min at 70 °C to obtain solution D.

[0097] After solution D was cooled to room temperature, glycerol, PPP peptide, phoenix skin polypeptide, sialic acid glycopeptide and phenoxyethanol were added sequentially while stirring. The mixture was then ultrasonically dispersed at a frequency of 40 kHz, a power of 200 W and a time of 45 min to obtain an egg-derived protein peptide composition that promotes skin elasticity and anti-aging.

[0098] Comparative Example 1

[0099] Compared with Example 1, Comparative Example 1 had no PPP peptide, but all other aspects were the same as in Example 1.

[0100] Comparative Example 2

[0101] Compared with Example 1, Comparative Example 2 did not contain the phoenix coat polypeptide, but was otherwise the same as Example 1.

[0102] Comparative Example 3

[0103] Compared with Example 1, Comparative Example 3 had no sialic acid glycopeptide, but all other aspects were the same as in Example 1.

[0104] The performance of the egg-derived protein peptide composition prepared in this application was evaluated, including:

[0105] 1. Stability Testing

[0106] The stability of the egg-derived protein peptide compositions prepared in Examples 1-4 was tested, as follows:

[0107] Heat resistance test: The constant temperature incubator was adjusted to 40℃. The samples of Examples 1-4 were placed in transparent glass bottles with a sample volume of 20ml / bottle. After sealing, the bottles were placed in the constant temperature incubator. After three months, the bottles were removed, allowed to return to room temperature, and the appearance changes were observed.

[0108] Cold resistance test: The constant temperature incubator was adjusted to -10℃, and the samples of Examples 1-4 were placed in transparent glass bottles with a sample volume of 20ml / bottle. After sealing, the bottles were placed in the constant temperature incubator. After three months, the bottles were removed, brought to room temperature, and the appearance changes were observed.

[0109] Room temperature test: The samples of Examples 1-4 were placed in transparent glass bottles with a sample volume of 20 ml / bottle. After sealing, the bottles were placed at room temperature for 6 months, and the appearance changes of the emulsion were observed.

[0110] The test results are shown in Table 1.

[0111] Table 1 Stability test results

[0112]

[0113] As shown in Table 1, no layering, precipitation, or discoloration was observed in the heat resistance test, cold resistance test, and room temperature test of the egg-derived protein peptide compositions prepared in Examples 1-4. They all maintained their original appearance and exhibited excellent high and low temperature stability.

[0114] 2. Antioxidant Test

[0115] DPPH, or 1,1-diphenyl-2-picrylhydrazyl free radical, is widely used to quantitatively determine the antioxidant capacity of biological samples. The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent; the higher the scavenging rate, the better its antioxidant effect and anti-aging effect. The anti-aging effect was determined by assessing the DPPH free radical scavenging ability of the egg-derived protein peptide compositions prepared in Example 1 and Comparative Examples 1-3.

[0116] The testing method is as follows:

[0117] Add 2 mL of 0.02 mg / L DPPH test solution and 2 mL of the protein peptide composition prepared in Examples 1 and Comparative Examples 1-3 to a test tube respectively. Make up the total volume with anhydrous ethanol to 5 mL, shake well, and react in the dark for a period of time. Measure the absorbance at 517 nm wavelength using a 1 cm cuvette and record it as As. Add 2 mL of DPPH test solution and 3 mL of anhydrous ethanol to a test tube and record the absorbance as A0. Add 2 mL of anhydrous ethanol and the corresponding volume of protein peptide composition to a test tube and make up the total volume with anhydrous ethanol to 5 mL. Record the absorbance as A sample.

[0118] The DPPH free radical scavenging rate (Y) is calculated using the formula: Y(%) = 1 - (As - A sample) / A0 × 100%. The test results are shown in Table 2.

[0119] Table 2. Experimental results of DPPH free radical scavenging

[0120]

[0121] As shown in Table 2, the egg-derived protein peptide composition prepared in Example 1 had a DPPH free radical scavenging rate of 91.54±0.38%, which was higher than that of Comparative Examples 1, 2 and 3 (79.42±0.67%, 76.59±1.10%, and 82.36±0.49%, respectively). This indicates that the combination of PPP peptide, phoenix skin peptide and sialic acid glycopeptide significantly improved the free radical scavenging rate, and they could synergistically enhance antioxidant capacity, reduce oxidative stress damage in the body, and exert anti-aging effects.

[0122] 3. Elastase inhibition test

[0123] The principle of the elastase inhibition assay is as follows: elastase reacts with its substrate, and the absorbance changes after the addition of the active substance. The magnitude of this change in absorbance reflects the inhibition rate of the elastase inhibitor. By detecting the inhibition rate of egg-derived protein peptide compositions on elastase, the anti-wrinkle efficacy achieved through elastase inhibition is analyzed and evaluated. The specific test method is as follows:

[0124] The egg-derived protein peptide composition was diluted to 50% with 0.2M borate buffer solution to serve as the test sample solution.

[0125] Add 100 μL of 2 mM Tris-HCl buffer, 10 μL of elastase solution, and 30 μL of sample solution to a 96-well plate and mix well (positive control: 100 μg / μL EGCG; blank control: Tris-HCl buffer). Each group has three replicates. Incubate at 25°C for 15 min. Add 40 μL of substrate AAAPVN to both the sample and control groups and incubate at 25°C for another 20 min. Measure the OD value at 405 nm using a microplate reader. The results are shown in Table 3. Figure 1 As shown.

[0126] Table 3. Results of elastase inhibition experiments

[0127]

[0128] From Table 3 and Figure 1 It can be seen that the egg-derived protein peptide composition prepared in Example 1 showed the highest inhibition rate of 63.76±0.41% against elastase, indicating the best effect. The elastase inhibition rates of the preparations in Examples 1-3 were all higher than those in Comparative Examples 1-3, demonstrating that the combination of PPP peptide, phoenix skin polypeptide, and sialic acid glycopeptide has a synergistic effect on elastase inhibition, which can slow down the degradation rate of elastin, maintain skin elasticity, and thus achieve an anti-wrinkle effect.

[0129] 4. Type III collagen expression test

[0130] Type III collagen is an important type of collagen found in tissues such as skin, blood vessels, and internal organs, and is closely related to skin elasticity and repair capabilities. This test used fibroblasts as subjects to evaluate the effects of the egg-derived protein peptide compositions of Examples 1-3 and Comparative Examples 1-3 on type III collagen expression. The specific experimental steps are as follows:

[0131] Cell seeding: Human fibroblasts with a confluence of 80% were seeded into 24-well plates at a volume of 2 mL per well to achieve a cell density of 4 × 10⁴ cells / well.

[0132] Sample addition and culture: Discard the culture medium, wash with PBS, and add high-glucose DMEM complete medium (blank group), high-glucose DMEM complete medium containing the reagents of the examples / comparative examples (sample group, the reagent content in the high-glucose DMEM complete medium is 0.1 wt%), and high-glucose DMEM complete medium containing the positive control (0.01 wt% ascorbic acid AA) (control group). Then place the well plate in a 37°C, 5% CO2 incubator for 72 h. The test results are shown in Table 4.

[0133] Table 4. Results of Type III Collagen Expression Experiment

[0134]

[0135] As shown in Table 4, the egg-derived protein peptide compositions prepared in Examples 1-3 all enhanced the content of type III collagen (p<0.05), with Example 1 showing the best effect. The egg-derived protein peptide compositions in Comparative Examples 1-3 did not significantly enhance collagen production. This indicates that the combination of PPP peptide, phoenix skin polypeptide, and sialic acid glycopeptide promotes the expression of type III collagen, thereby increasing skin elasticity, maintaining skin health, delaying aging, and improving skin damage.

[0136] 5. Safety test

[0137] Safety tests were conducted on the egg-derived protein peptide compositions prepared in Examples 1-4, with 40 volunteers selected for the tests.

[0138] The specific testing procedure is as follows: The test substance is placed in a patch applicator, with a dosage of approximately 0.020–0.025 g. The patch applicator containing the test substance is then applied to the back or flexor side of the forearm of the subject using non-irritating adhesive tape. Gently press with the palm of the hand to ensure even application to the skin, and leave for 24 hours. After removing the patch applicator, observe the skin reaction 30 minutes after the pressure mark disappears. If the result is negative, repeat the observation at 24 hours and 48 hours after the patch test. The grading criteria for skin reaction in the closed patch test are shown in Table 5, and the statistical results of the skin patch test are shown in Table 6.

[0139] Table 5. Grading Criteria for Skin Reactions in Closed Patch Tests

[0140]

[0141]

[0142] Table 6. Statistical Table of Skin Patch Test Results

[0143]

[0144] As shown in Table 6, the irritation levels of the egg-derived protein peptide compositions prepared in Examples 1 to 4 are all non-irritating, indicating that the compositions are mild and non-irritating, will not cause adverse skin reactions, and have high safety.

[0145] Although this application has been described in detail in this specification with general descriptions and specific embodiments, some modifications or improvements can be made to it, which will be obvious to those skilled in the art. Therefore, such modifications or improvements made without departing from the spirit of this application are all within the scope of protection claimed in this application.

Claims

1. An egg-derived protein peptide composition for promoting skin elasticity and anti-aging, characterized in that, comprises, by mass percentage: 0.1-10wt% of the PPP peptide, 0.1-5wt% of the Fenghuangyi polypeptide, 0.00001wt% of the sialic acid glycopeptide, 2-10wt% of the humectant, 0.1-5wt% of the penetration enhancer, 0.1-5wt% of the thickening agent, 2-8wt% of the emulsifier, 0.1-0.3wt% of disodium EDTA, 0.01-0.5wt% of the preservative, and the balance being water; the penetration enhancer is at least one of isosorbide dimethyl ether, inositol, or betaine.

2. The chicken egg-derived protein peptide composition according to claim 1, characterized by, comprises, by mass percentage: 5-10wt% of the PPP peptide, 3-5wt% of the Fenghuangyi polypeptide, 0.00001wt% of the sialic acid glycopeptide, 2-10wt% of the humectant, 0.1-5wt% of the penetration enhancer, 0.1-5wt% of the thickening agent, 2-8wt% of the emulsifier, 0.1-0.3wt% of disodium EDTA, 0.01-0.5wt% of the preservative, and the balance being water.

3. The chicken egg-derived protein peptide composition according to claim 1, characterized by, the humectant is at least one of glycerin, propylene glycol, butylene glycol, sodium hyaluronate, or panthenol.

4. The chicken egg-derived protein peptide composition according to claim 1, characterized by, the thickening agent is acrylates / C10-30 alkyl acrylate crosspolymer.

5. The chicken egg-derived protein peptide composition according to claim 1, characterized by, the emulsifier is polysorbate-20 or polyglyceryl-10 myristate.

6. The chicken egg-derived protein peptide composition according to claim 1, characterized by, the preservative is phenoxyethanol or p-hydroxyacetophenone.

7. The chicken egg-derived protein peptide composition according to claim 1, characterized by, comprises, by mass percentage: 10wt% of the PPP peptide, 5wt% of the Fenghuangyi polypeptide, 0.00001wt% of the sialic acid glycopeptide, 5wt% of glycerin, 1wt% of panthenol, 0.1wt% of sodium hyaluronate, 2.2wt% of isosorbide dimethyl ether, 1.8wt% of inositol, 3wt% of acrylates / C10-30 alkyl acrylate crosspolymer, 6wt% of polyglyceryl-10 myristate, 0.2wt% of disodium EDTA, 0.5wt% of phenoxyethanol, and the balance being water.

8. The method for preparing the egg-derived protein peptide composition for promoting skin elasticity and anti-aging according to claim 1, characterized in that, comprises: dispersing the thickening agent in a proper amount of deionized water, stirring uniformly to obtain solution A; adding disodium EDTA into the remaining deionized water, stirring uniformly to obtain solution B; mixing solution A and solution B uniformly to obtain solution C; adding the penetration enhancer and the emulsifier into solution C in sequence, stirring uniformly at 70-90°C to obtain solution D; cooling solution D to room temperature, adding the humectant, the PPP peptide, the Fenghuangyi polypeptide, the sialic acid glycopeptide, and the preservative while stirring, and ultrasonic dispersing to obtain the chicken egg-derived protein peptide composition for promoting skin elasticity and anti-aging.

9. Use of the chicken egg-derived protein peptide composition for promoting skin elasticity and anti-aging according to any one of claims 1-7 in cosmetics.

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