Special device for constructing brain injury mouse based on cortical control strike model

Abstract

The invention provides a special device for constructing a brain injury mouse based on a cortical control strike model, and belongs to the technical field of medical instruments. The device comprises an impactor base, a movable supporting plate is installed on the outer wall of the top of the impactor base, an experiment base is fixedly connected to the outer wall of the top of the movable supporting plate, a supporting frame is fixedly connected to the outer wall of the top of the experiment base, and first clamping grooves, first sliding grooves and second clamping grooves are symmetrically formed in the two sides of the top of the experiment base; the first sliding groove is located between the first clamping groove and the second clamping groove, and a second sliding groove is formed in the middle of the top of the experiment base; the fixing assembly is used for fixing a mouse, and the fixing assembly is connected with the experiment base. By arranging the fixing assembly, the limb fixing device can adapt to the thicknesses of limbs of different mice and fix the limbs of the mice, the fixing mode is simple and rapid, the experiment time is saved, the cranial crest can be located at the horizontal position through adjustment, and the local fracture probability is reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of medical appliances, and in particular to a special device for constructing brain-injured mice based on a cortical-controlled blow model. Background Art

[0002] Traumatic brain injury (TBI) refers to damage to the brain caused by external mechanical forces, and its clinical incidence is increasing year by year. Establishing a robust animal model is crucial for in-depth investigation of the pathogenesis of this condition, a comprehensive understanding of its pathophysiology, and the development of novel treatment options.

[0003] In current research, there are four main widely used models of brain trauma: the liquid impact injury model, the controlled cortical concussion injury (CCI) model, the free-fall acceleration injury model, and the blast injury model. Among them, the CCI model is widely used in traumatic brain injury (TBI) research due to its controllable impact speed, depth, and time. However, the classic CCI model has limitations. It requires a craniotomy before the impact, which undoubtedly adds additional trauma and is inconsistent with the common brain trauma in daily life. Therefore, in animal experiments on post-traumatic headache, the free-fall acceleration injury model is more popular among researchers. The advantage of this model is that it does not require a craniotomy and can induce diffuse brain damage during the impact.

[0004] At present, when constructing a concussion model through CCI, a sponge is placed under the anesthetized mouse, and then the mouse's body is fixed with medical tape. However, this device has the following shortcomings: (1) The top of the mouse's skull is significantly tilted, not perpendicular to the direction of the blow, which reduces the impact area and increases the probability of local fractures; (2) The mouse's head is not fixed and moves significantly during the blow, which reduces the effectiveness of the vertical blow, reduces the brain damage caused by rotation, and increases the uncertainty of the blow. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to provide a special device for constructing brain-injured mice based on a cortical-controlled impact model. By setting up fixing components, it can not only adapt to the thickness of the limbs of different mice and fix their limbs, but also the fixing method is simple and quick, saving experimental time. In addition, with the cooperation of various components, it can adapt to the torsos of different mice so that they fit on the surface of the experimental base, and then adjust the top of the skull to a horizontal position to reduce the chance of local fractures. The above settings can solve the problem that the mouse head is not fixed, moves significantly during the impact, and increases additional trauma.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] A special device for constructing brain-injured mice based on a cortical-controlled strike model includes a striker base, a movable support plate is installed on the top outer wall of the striker base, an experimental base is fixedly connected to the top outer wall of the movable support plate, a support frame is fixedly connected to the top outer wall of the experimental base, a first clamping groove, a first sliding groove and a second clamping groove are symmetrically provided on both sides of the top of the experimental base, and the first sliding groove is located in the middle of the first clamping groove and the second clamping groove, and a second sliding groove is provided in the middle of the top of the experimental base; a fixing component, the fixing component is used to fix the mouse, and the fixing component is connected to the experimental base.

[0008] Optionally, the fixing assembly includes a first limit block, a second limit block and a third limit block which are symmetrically fixedly connected to both sides of the top outer wall of the experimental base, wherein the first limit block and the second limit block are respectively located on both sides of the first clip groove, and the third limit block is located on one side of the second clip groove.

[0009] Optionally, the first limit block is provided with several first segmented grooves on the outer wall close to the second sliding groove, the first limit block is provided with a first avoidance groove on the outer wall close to the first clamping groove, the second limit block is provided with a second avoidance groove on the outer wall close to the first clamping groove, and the third limit block is provided with a third avoidance groove on the outer wall close to the second clamping groove.

[0010] Optionally, an S-shaped elastic plate is fixedly connected to the bottom inner wall of the first clamping slot and the second clamping slot respectively, the two S-shaped elastic plates in each group are distributed in a mirror image, one end of the two S-shaped elastic plates is fixedly connected to a fixing plate, and a plurality of limiting holes are opened in the middle of the top of the fixing plate.

[0011] Optionally, a support plate is fixedly connected to one side of the top outer wall of the fixing plate, one end of the support plate is fixedly connected to a C-shaped fixing plate, a first pull cable is fixedly connected to the outer wall of the support plate close to the C-shaped fixing plate, the C-shaped fixing plate is provided with a fourth avoidance groove running through both ends thereof, and the fourth avoidance groove is fixedly connected to the same fixing ring on both sides of the inner walls close to the bottom of the C-shaped fixing plate.

[0012] Optionally, one end of the first cable is fixedly connected to a rotating cylinder, a limit pin is rotatably connected to the inner wall of the rotating cylinder, a fixed circular plate is fixedly connected to the outer wall of the limit pin near the top of the rotating cylinder, a limit clamping plate is fixedly connected to the outer wall of the limit pin near the top of the rotating cylinder, and an indicator arrow is provided on the top outer wall of the fixed circular plate.

[0013] Optionally, a sliding plate is slidably connected to the inner wall of the second sliding groove, a second circular hole is opened on the outer wall of one end of the sliding plate, a head pad is fixedly connected to the end of the top of the sliding plate away from the second circular hole, and a rotating groove is opened on the outer wall of the head pad on the side close to the support frame.

[0014] Optionally, a fourth limit block is slidably connected to the inner wall of the first sliding groove, and a plurality of second segmented grooves are provided on the outer wall of the fourth limit block close to the second sliding groove, and a third sliding groove is symmetrically provided on the outer walls on both sides of the fourth limit block close to the second limit block and the third limit block.

[0015] Optionally, a fixed protrusion is fixedly connected to the bottom outer wall of the fourth limit block, and a spring is fixedly connected to the outer wall of the fixed protrusion close to the second sliding groove, wherein the other end of the spring is fixedly connected to the inner wall of the first sliding groove close to the second sliding groove.

[0016] Optionally, a first circular hole is formed on the inner wall of the first sliding groove close to the second sliding groove, the fixing protrusion is fixedly connected to the second cable on the outer wall close to the spring, and the second cable passes through the first circular hole and the second circular hole in sequence.

[0017] Compared with the prior art, the present invention has at least the following beneficial effects:

[0018] In the above scheme, by setting up fixing components, not only can the thickness of the limbs of different mice be adapted and their limbs be fixed, but the fixing method is simple and quick, saving experimental time. With the cooperation of various components, the torsos of different mice can be adapted to fit the surface of the experimental base, and then the top of the skull can be adjusted to a horizontal position to reduce the chance of local fractures.

[0019] By setting the first limit block, the second limit block and the third limit block in the fixing component, it can not only adapt to the head contour of the mouse, but also provide just the right avoidance space when the experimenter fixes the mouse's limbs, making it convenient for the experimenter to operate.

[0020] By arranging an S-shaped elastic plate, a C-shaped fixing plate, a fixing ring, a first pull rope, a limiting pin and a limiting snap-on plate in the fixing assembly, the limbs of the mouse placed on the top outer wall of the C-shaped fixing plate can be fixed, and the thickness of the limbs of different mice can be adapted by adjusting the tightness of the first pull rope. In addition, the limiting pin can be pressed and rotated to make the mouse's torso fit against the top outer wall of the experimental base and maintain a horizontal state, thereby avoiding accidental trauma during the striking experiment.

[0021] By setting a head pad in the fixed component, not only can a certain cushioning force be given to the mouse's head during the impact experiment to prevent its head from being subjected to recoil, but the height of the mouse's head can also be adjusted by turning the threaded column to keep the top of the skull in a horizontal position, reducing the chance of local fractures.

[0022] By arranging a sliding plate, a fourth limit block, a fixed protrusion, a spring and a second cable in the fixing assembly, not only can the sliding plate be driven to slide by rotating the threaded column to fix the mouse's torso, but it can also adapt to the contours of the two sides of the torso of different mice. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable one skilled in the art to make and use the invention.

[0024] Figure 1 A schematic diagram of the three-dimensional structure of a special device for constructing a brain-injured mouse based on the cortical-controlled impact model of the present invention;

[0025] Figure 2 This is an enlarged three-dimensional structural diagram of the experimental base, the first limiting block, the fixing plate and the head pad of the present invention;

[0026] Figure 3 This is an enlarged three-dimensional structural diagram of the experimental base and support frame of the present invention;

[0027] Figure 4 for Figure 3 A in the middle is an enlarged schematic diagram of the three-dimensional structure;

[0028] Figure 5 This is a schematic diagram of the enlarged three-dimensional structure of the experimental base, the first limiting block, the second limiting block and the third limiting block of the present invention in cooperation with a first viewing angle;

[0029] Figure 6 This is a schematic diagram of the magnified three-dimensional structure of the experimental base, the first limiting block, the second limiting block and the third limiting block of the present invention in a second viewing angle;

[0030] Figure 7 This is an enlarged three-dimensional structural diagram of the fixing plate, S-shaped elastic plate and limiting pins of the present invention;

[0031] Figure 8 This is an enlarged three-dimensional structural diagram of the fixing plate, the S-shaped elastic plate and the first cable of the present invention;

[0032] Figure 9 This is an enlarged three-dimensional structural diagram of the fixing plate, S-shaped elastic plate and support plate of the present invention;

[0033] Figure 10This is an enlarged three-dimensional structural diagram of the limiting needle, the fixed circular plate and the limiting clamping plate of the present invention;

[0034] Figure 11 This is an enlarged three-dimensional structural diagram of the headrest and sliding plate of the present invention;

[0035] Figure 12 This is an enlarged three-dimensional structural diagram of the fourth limit block, the fixing protrusion, the spring and the second cable of the present invention;

[0036] Figure 13 It is a schematic diagram of an enlarged three-dimensional structure of the fourth limiting block and the fixing protrusion of the present invention.

[0037] Reference numerals:

[0038] 1. Beater base; 101. Moving support plate; 2. Experimental base; 201. First clamping slot; 202. First sliding slot; 203. Second clamping slot; 204. Second sliding slot; 205. Support frame; 2051. Clamping portion; 2052. Support portion; 206. Threaded column; 2061. Screw connection portion; 2062. Propelling portion; 2063. Rotating portion; 207. First circular hole; 3. First limiting block; 301. First segmented slot; 302. First avoidance slot; 303. Second avoidance slot; 304. Second limiting block; 305. Third limiting block; 306. Third avoidance slot; 307. Sliding Dynamic protrusion; 4. Fixed plate; 401. S-shaped elastic plate; 402. Support plate; 403. C-shaped fixed plate; 404. Fourth avoidance groove; 405. Fixed ring; 4051. Connecting part; 406. First cable; 407. Rotating cylinder; 408. Limiting hole; 5. Head pad; 501. Rotating groove; 502. Sliding plate; 503. Second circular hole; 6. Limiting needle; 601. Fixed circular plate; 602. Limiting clamping plate; 603. Indicating arrow; 7. Fourth limiting block; 701. Second segmented groove; 702. Third sliding groove; 703. Fixed protrusion; 704. Spring; 705. Second cable.

[0039] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments. DETAILED DESCRIPTION

[0040] The following, in conjunction with the accompanying drawings and specific examples, describes in detail a specialized device for constructing a cortical-controlled percussion model for brain-injured mice. It is also noted that, for the sake of completeness, the following examples are best and preferred embodiments, and those skilled in the art may employ alternative methods for implementing known techniques. Furthermore, the accompanying drawings are provided solely for the purpose of describing the embodiments in greater detail and are not intended to limit the present invention.

[0041] It should be noted that references in the specification to "one embodiment," "an embodiment," "exemplary embodiments," "some embodiments," etc. indicate that the described embodiments may include specific features, structures, or characteristics, but not necessarily every embodiment will include such specific features, structures, or characteristics. Furthermore, when specific features, structures, or characteristics are described in conjunction with an embodiment, it is within the knowledge of persons skilled in the relevant art to implement such features, structures, or characteristics in conjunction with other embodiments (whether or not explicitly described).

[0042] In general, terms can be understood, at least in part, from their use in context. For example, depending at least in part on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending at least in part on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0043] It will be understood that the meanings of “on,” “over,” and “above” in the present invention should be interpreted in the broadest manner, so that “on” means not only “directly on” something but also includes the meaning of being “on” something with intervening features or layers, and “on” or “above” means not only “on” or “above” something but also includes the meaning of being “on” or “above” something with no intervening features or layers.

[0044] Additionally, spatially relative terms such as "below," "beneath," "lower," "above," and "upper" may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as illustrated in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein should be similarly interpreted accordingly.

[0045] like Figures 1 to 13As shown, an embodiment of the present invention provides a special device for constructing a brain-injured mouse based on a cortical-controlled strike model, comprising a striker base 1, a movable support plate 101 being mounted on the top outer wall of the striker base 1, an experimental base 2 being fixedly connected to the top outer wall of the movable support plate 101, a support frame 205 being fixedly connected to the top outer wall of the experimental base 2, a first snap-in groove 201, a first sliding groove 202, and a second snap-in groove 203 being symmetrically provided on both sides of the top of the experimental base 2, and the first sliding groove 202 being located between the first snap-in groove 201 and the second snap-in groove 203, and a second sliding groove 204 being provided in the middle of the top of the experimental base 2. Specifically, the experimental base 2 is fixedly connected to the top outer wall of the movable support plate 101, and the experimental base 2 is a rectangular parallelepiped made of polyurethane foam. The elastic polyurethane foam experimental base 2 can provide a shock-absorbing effect to the mouse during the strike experiment, thereby preventing the mouse from suffering secondary trauma.

[0046] The support frame 205 is fixedly connected to the top outer wall of the experimental base 2. The support frame 205 is made of plastic and consists of two parts, namely a "C"-shaped clamping portion 2051 and a rectangular support portion 2052 with a threaded hole in the center. When the clamping portion 2051 is subjected to force, it will deform along the direction of its bending, and can clamp the anesthesia mask used in the experiment, thereby fixing the anesthesia mask. One end of the support portion 2052 is fixedly connected to the outer wall of the clamping portion 2051 close to the experimental base 2, and the other end of the support portion 2052 is fixedly connected to a position near the middle of the top outer wall of the experimental base 2. In this way, when anesthetizing the mouse, vibration caused by hitting the mouse's head can be avoided, which may cause the anesthesia mask to shift. The distance between the anesthesia mask and the mouse's mouth and nose can also be adjusted by adjusting the position of the clamping portion 2051 on the outer wall of the anesthesia mask. The threaded column 206 is screwed into the threaded hole set on the support frame 205. The threaded column 206 is made of plastic and consists of three parts, namely a circular plate-shaped propulsion part 2062, a cylindrical screw-connecting part 2061 with a threaded groove on the outer wall, and a cylindrical rotating part 2063 with an anti-slip groove on the outer wall. The screw-connecting part 2061 is screwed into the threaded hole set on the support frame 205, and the propulsion part 2062 and the rotating part 2063 are respectively fixedly connected to the two ends of the screw-connecting part 2061; when the rotating part 2063 on the threaded column 206 is rotated clockwise, the propulsion part 2062 on the threaded column 206 will rotate and move toward the direction of the central axis of the experimental base 2. Conversely, when the rotating part 2063 of the threaded column 206 is rotated counterclockwise, the propulsion part 2062 on the threaded column 206 will rotate and move away from the central axis of the experimental base 2.

[0047] like Figure 3As shown, two first clamping grooves 201, two first sliding grooves 202 and two second clamping grooves 203 are symmetrically opened on the top of the experimental base 2, wherein the first sliding groove 202 is located in the middle position of the first clamping groove 201 and the second clamping groove 203, the first clamping groove 201 and the second clamping groove 203 are both "convex" shaped grooves, and the inner wall on the side close to the middle of the experimental base 2 has an arc, the first sliding groove 202 is a rectangular groove, and the second sliding groove 204 is opened in the middle position of the top of the experimental base 2, and the second sliding groove 204 consists of three parts, a long and thin rectangular groove at one end close to the support frame 205, a middle rectangular groove and a thicker rectangular parallelepiped groove with an arc at the other end, and the overall outline is in the shape of a "middle".

[0048] The present invention also includes a fixation assembly for securing mice. Connected to the experimental base 2, this assembly allows for immobilization during CCI modeling experiments, minimizing trauma from vertical impacts and providing technical support for fundamental research on brain injury. This fixation assembly not only accommodates the limbs of different mice, but also allows for a simple and rapid fixation method, saving experimental time. Furthermore, the components, when combined, allow the torso of each mouse to fit snugly against the surface of the experimental base 2. Adjustment can then maintain a horizontal position for the skull, reducing the risk of localized fractures.

[0049] like Figures 1 to 11 As shown, the fixing assembly includes a first limit block 3, a second limit block 304 and a third limit block 305 which are symmetrically fixedly connected to both sides of the top outer wall of the experimental base 2, wherein the first limit block 3 and the second limit block 304 are respectively located on both sides of the first clamping groove 201, and the third limit block 305 is located on one side of the second clamping groove 203. A plurality of first segmented grooves 301 are provided on the outer wall of the first limit block 3 near the second sliding groove 204, a first avoidance groove 302 is provided on the outer wall of the first limit block 3 near the first clamping groove 201, a second avoidance groove 303 is provided on the outer wall of the second limit block 304 near the first clamping groove 201, and a third avoidance groove 306 is provided on the outer wall of the third limit block 305 near the second clamping groove 203.

[0050] Specifically, the fixing assembly includes two first limit blocks 3, two second limit blocks 304 and two third limit blocks 305, which are respectively symmetrically fixed on both sides of the top outer wall of the experimental base 2, wherein the first limit block 3 is located on the side of the first clamping groove 201 close to the support frame 205, the second limit block 304 is located on the other side of the first clamping groove 201, and the third limit block 305 is located on the side of the second clamping groove 203 close to the first clamping groove 201. The first limit block 3 is made of polyurethane foam and has a trapezoidal profile as a whole, and the top outer wall and the second sliding groove 204 are close to each other. One side of the outer wall is provided with a curvature, and several first segmented grooves 301 are opened on the outer wall of the first limit block 3 near the second sliding groove 204, and are distributed in an arc array. The first segmented groove 301 is a rectangular groove body with a curvature, which, together with the curvature of the outer wall of the first limit block 3 near the second sliding groove 204, can further adapt to the head contour of the mouse. The second limit block 304 and the third limit block 305 are both made of polyurethane foam and have a trapezoidal outline as a whole. The top outer wall of the second limit block 304 and the third limit block 305 and the outer wall near the second sliding groove 20 4 is provided with an arc on one side of the outer wall, but the volume of the second limit block 304 is smaller than that of the third limit block 305, wherein the two sliding protrusions 307 are symmetrically fixedly connected to the outer wall of the second limit block 304 near the first sliding groove 202 and the outer wall of the third limit block 305 near the first sliding groove 202, respectively. The sliding protrusion 307 is a polyurethane foam cuboid, and the first avoidance groove 302 and the second avoidance groove 303 are respectively opened on one side of the top outer wall of the first limit block 3 near the first clamping groove 201 and the one side of the top outer wall of the second limit block 304 near the first clamping groove 201. On the other hand, the first avoidance groove 302 and the second avoidance groove 303 are both arc-shaped grooves. The cooperation between the two can provide avoidance space for the experimenter when fixing the mouse's forelimbs, which is convenient for the experimenter to operate. The third avoidance groove 306 is opened on the side of the top outer wall of the third limit block 305 close to the second clamping groove 203. It is an arc-shaped groove, which can provide avoidance space for the experimenter when fixing the mouse's hind limbs. The above structural setting can not only adapt to the mouse's head contour, but also provide just the right avoidance space when the experimenter fixes the mouse's limbs, which is convenient for the experimenter to operate.

[0051] like Figures 1 to 10As shown, two groups of S-shaped elastic plates 401 are fixedly connected to the bottom inner walls of the first clamping slot 201 and the second clamping slot 203, respectively. The two S-shaped elastic plates 401 in each group are distributed in a mirror image. One end of the two S-shaped elastic plates 401 is fixedly connected to a fixing plate 4. A plurality of limiting holes 408 are opened in the middle of the top of the fixing plate 4. A support plate 402 is fixedly connected to one side of the top outer wall of the fixing plate 4. One end of the support plate 402 is fixedly connected to a C-shaped fixing plate 403. A first cable 406 is fixedly connected to the outer wall of the support plate 402 close to the C-shaped fixing plate 403. The C-shaped fixing plate 403 is provided with a fourth avoidance groove 404 running through both ends thereof, and the same fixing ring 405 is fixedly connected to the inner walls on both sides of the fourth avoidance groove 404 near the bottom of the C-shaped fixing plate 403, and one end of the first cable 406 is fixedly connected to the rotating cylinder 407, and the inner wall of the rotating cylinder 407 is rotatably connected to the limit pin 6, and the outer wall of the limit pin 6 is fixedly connected to a fixed circular plate 601 near the top of the rotating cylinder 407, and the outer wall of the limit pin 6 is fixedly connected to a limit clamping plate 602 near the top of the rotating cylinder 407, and an indicator arrow 603 is provided on the top outer wall of the fixed circular plate 601.

[0052] Specifically, two groups of S-shaped elastic plates 401 are respectively fixedly connected to the bottom inner walls of the first clamping groove 201 and the second clamping groove 203. The two S-shaped elastic plates 401 in each group are distributed in a mirror image. The S-shaped elastic plate 401 is an "S"-shaped plastic plate. When the S-shaped elastic plate 401 is subjected to force, it will deform along the direction of its bending. The two groups of S-shaped elastic plates 401 are respectively fixedly connected to the bottom outer walls of the two fixed plates 4. The fixed plate 4 is a rectangular plastic plate with an arc at one end and an inclined edge at the other end. Several limiting holes 408 are distributed in a linear array and are opened in the middle position of the top outer wall of the fixed plate 4 near one end of the inclined edge. The limiting hole 408 is a circular groove body. One end of the support plate 402 is fixedly connected to the side of the outer wall of the top outer wall of the fixed plate 4 near the end with the arc. The support plate 402 is a "7"-shaped plastic Plate, one end of the C-shaped fixing plate 403 is fixedly connected to the end of the support plate 402 away from the fixing plate 4, the C-shaped fixing plate 403 is a "C"-shaped plastic plate, and the C-shaped fixing plate 403 cooperates with the support plate 402 to support the limbs of the mouse, and the C-shaped fixing plate 403 is provided with a fourth avoidance groove 404 running through both ends thereof, the fourth avoidance groove 404 is a square groove body, and the fixing ring 405 is fixedly connected to the bottom outer wall of the C-shaped fixing plate 403; the fixing ring 405 is made of plastic material and consists of two parts, namely, a plastic ring and a rectangular parallelepiped-shaped connecting part 4051, wherein one end of the connecting part 4051 on the fixing ring 405 is fixedly connected to the outer wall of the plastic ring, and the two sides of the connecting part 4051 are respectively fixedly connected to the inner walls on both sides of the fourth avoidance groove 404 near the bottom of the C-shaped fixing plate 403.

[0053] When the fixing plates 4 on the first clamping slot 201 and the second clamping slot 203 are pressed respectively, the two groups of S-shaped elastic plates 401 will be subjected to pressing pressure and deform along the direction of their bending, and the support plate 402, C-shaped fixing plate 403 and fixing ring 405 on the first clamping slot 201 and the second clamping slot 203 will respectively follow the fixing plates 4 on the first clamping slot 201 and the second clamping slot 203 to move in the direction of pressing. One end of the first cable 406 is fixedly connected to the outer wall of one side of the support plate 402 close to the C-shaped fixing plate 403. The first cable 406 is disclosed in the prior art and will not be described in detail. The first cable 406 is made of metal, which is tough and wear-resistant. The other end of the cable first passes through the fourth avoidance groove 404, then passes through the fixing ring 405 and is fixedly connected to the outer wall of the rotating cylinder 407. The rotating cylinder 407 is a plastic cylinder. By pulling the rotating cylinder 407 to tighten the first cable 406, the limbs of the mouse placed on the top outer wall of the C-shaped fixing plate 403 can be fixed. The tightness of the first cable 406 can also be adjusted to adapt to the thickness of the limbs of different mice.

[0054] Specifically, the limit pin 6 is rotatably connected to the inner wall of the rotating cylinder 407. The limit pin 6 consists of two parts, namely a plastic elliptical cylinder with an anti-slip groove and a plastic cylinder with a chamfered end. A fixed circular plate 601 is fixedly connected to the outer wall of the plastic cylinder near the top of the rotating cylinder 407. The fixed circular plate 601 is a plastic circular plate. The indicator arrow 603 is set on the top outer wall of the fixed circular plate 601. The limit clamping plate 602 is fixedly connected to the limit pin 6. The outer wall of the plastic cylinder is close to the rotating cylinder 407. At the bottom, the limiting snap-in plate 602 is a plastic cuboid with curved ends, and the length of the limiting snap-in plate 602 is respectively adapted to the width of the bottom groove body of the first snap-in groove 201 and the width of the bottom groove body of the second snap-in groove 203, wherein the extension direction of the limiting snap-in plate 602 is consistent with the indication direction of the indicating arrow 603, and the contour of the plastic cylinder on the limiting needle 6 is adapted to the contour of the limiting hole 408, and the limiting needle 6 can be used to insert the limiting holes 408 at different positions to adjust the tightness of the first cable 406.

[0055] When the experimenter places the mouse's limbs on the top outer walls of the four C-shaped fixing plates 403 respectively, and then inserts the limiting pins 6 into the appropriate limiting holes 408 according to the thickness of the mouse's limbs, the mouse's limbs can be fixed. Then continue to press the limiting pins 6. Under the action of the limiting clamping plate 602, the fixing plate 4 on the first clamping groove 201 will be driven to move toward the bottom of the first clamping groove 201, and the fixing plate 4 on the second clamping groove 203 will move toward the bottom of the second clamping groove 203. At this time, the S-shaped elastic plate 401 will be deformed along the direction of its bending after being pressed. At this time, the fixed mouse limbs will also move with the fixing plate 4, and the mouse torso will move with its limbs until the mouse torso fits on the top outer wall of the experimental base 2. Stop pressing the limiting pins 6 and then rotate 90 degrees. The limiting pin 6 is perpendicular to the fixing plate 4 at this time. Since the length of the limiting snap-in plate 602 is adapted to the width of the bottom groove of the first snap-in groove 201, the experimenter releases the hand pressing the limiting pin 6, and the S-shaped elastic plate 401 is no longer under force. It will restore its deformation under the action of its own elasticity, pushing the fixing plate 4 to move toward the top of the experimental base 2. The limiting snap-in plate 602 perpendicular to the fixing plate 4 on the first snap-in groove 201 will be stuck on the inner wall near the top of the first snap-in groove 201, and the limiting snap-in plate 602 perpendicular to the fixing plate 4 on the second snap-in groove 203 will be stuck on the inner wall near the top of the second snap-in groove 203. The limiting snap-in plate 602 will respectively prevent the fixing plates 4 on the first snap-in groove 201 and the second snap-in groove 203 from continuing to move. At this time, the mouse's torso just fits on the top outer wall of the experimental base 2.

[0056] When the experiment is completed and the mouse needs to be taken out, the limiting pin 6 can be rotated 90 degrees again to release the limit of the limiting clamping plate 602. The S-shaped elastic plate 401 can restore its deformation under the elasticity of the S-shaped elastic plate 401 itself, pushing the fixing plate 4 to move toward the top of the experimental base 2, and the mouse will also move with the fixing plate 4. At this time, the limiting pin 6 can be pulled out to loosen the first cable 406 and release the fixation of the mouse's limbs. The above structural setting can fix the mouse limbs placed on the top outer wall of the C-shaped fixing plate 403, and can also adapt to the thickness of different mouse limbs by adjusting the tightness of the first cable 406. In addition, the limiting pin 6 can be pressed and rotated to make the mouse's torso fit against the top outer wall of the experimental base 2 and maintain a horizontal state to avoid accidental trauma during the impact experiment.

[0057] like Figures 1 to 11As shown, a sliding plate 502 is slidably connected to the inner wall of the second sliding groove 204, and a second circular hole 503 is provided on the outer wall of one end of the sliding plate 502. The end of the top of the sliding plate 502 away from the second circular hole 503 is fixedly connected to the head pad 5, and a rotating groove 501 is provided on the outer wall of the head pad 5 close to the support frame 205. Specifically, the sliding plate 502 is slidably connected to the inner wall of the second sliding groove 204. The sliding plate 502 is a plastic cuboid with an arc at one end. The overall contour of the sliding plate 502 is adapted to the inner wall contour of the second sliding groove 204, so the sliding plate 502 can slide on the inner wall of the second sliding groove 204. The second circular hole 503 is provided on the sliding plate 5 02 is on a section of the outer wall with an arc, the second circular hole 503 is a circular groove body, and the head pad 5 is fixedly connected to the outer wall of the top of the sliding plate 502 away from the second circular hole 503. The head pad 5 is composed of two parts, which are divided into a polyurethane foam rectangle and a polyurethane foam triangular prism with arcs at both ends. Such an elastic polyurethane foam head pad 5 can provide a cushioning effect for the mouse's head during the impact experiment. The rotating groove 501 is opened on the outer wall of the side of the head pad 5 close to the support frame 205. The cross-section of the rotating groove 501 is a "convex" shape, and the overall outline is adapted to the outline of the threaded column 206 mentioned above. One end of the threaded column 206 can rotate on the inner wall of the rotating groove 501.

[0058] When the threaded column 206 is rotated clockwise, the threaded column 206 will rotate and push the head pad 5 to move away from the support frame 205, and will drive the sliding plate 502 to slide along the inner wall of the second sliding groove 204 in the direction away from the support frame 205. At this time, the inclined surface of the polyurethane foam triangular prism with arcs at both ends of the head pad 5 will lift the mouse's head, so that the part of the mouse's head that is hit can be kept level through fine-tuning, and at the same time, the anesthesia mask connected to the support frame 205 is delivered to the mouse's mouth and nose. If, during the fine-tuning process, the part of the mouse's head that is hit is already level, but there is still a distance between the mouth and nose and the anesthesia mask, the problem can be solved by adjusting the connecting position of the outer wall of the anesthesia mask and the support frame 205. When the experiment is over, the reversible When the threaded column 206 is rotated clockwise, the threaded column 206 will rotate and pull the head pad 5 to move toward the support frame 205, and will drive the sliding plate 502 to slide along the inner wall of the second sliding groove 204 toward the support frame 205. At this time, the inclined surface of the polyurethane foam triangular prism with curved ends on the head pad 5 will put the mouse's head down. At the same time, the anesthesia mask will be away from the mouse's mouth and nose to avoid hindering the removal of the mouse for observation after the experiment. The above structural setting can not only provide a certain buffering force to the mouse's head during the impact experiment to prevent its head from being subjected to recoil, but also can adjust the height of the mouse's head by rotating the threaded column 206 to keep the top of the skull in a horizontal position and reduce the chance of local fracture.

[0059] like Figures 1 to 11As shown, the fourth limit blocks 7 are symmetrically slidably connected on the inner walls of the two first sliding grooves 202, and several second segmented grooves 701 are opened on the outer wall of the fourth limit block 7 on one side close to the second sliding groove 204. The fourth limit block 7 is symmetrically opened on the outer walls on both sides close to the second limit block 304 and the third limit block 305 respectively. A fixing protrusion 703 is fixedly connected on the bottom outer wall of the fourth limit block 7, and a spring 704 is fixedly connected on the outer wall of the fixing protrusion 703 close to the second sliding groove 204, wherein the other end of the spring 704 is fixedly connected to the inner wall of the first sliding groove 202 close to the second sliding groove 204, and a first circular hole 207 is opened on the inner wall of the first sliding groove 202 close to the second sliding groove 204, and a second cable 705 is fixedly connected on the outer wall of the fixing protrusion 703 close to the spring 704, and the second cable 705 passes through the first circular hole 207 and the second circular hole 503 in sequence.

[0060] Specifically, the two fourth limit blocks 7 are symmetrically slidably connected to the inner walls of the two first sliding grooves 202. The fourth limit block 7 is a polyurethane foam cuboid, and has an arc on the side close to the second sliding groove 204. Several second segmented grooves 701 are opened on the outer wall of the fourth limit block 7 on the side close to the second sliding groove 204, and are distributed in an arc array. In this way, the fourth limit block 7 made of polyurethane foam can further adapt to the contours of both sides of the mouse torso during the process of force deformation. The two third sliding grooves 702 are symmetrically opened on the outer walls of both sides of the fourth limit block 7 close to the second limit block 304 and the third limit block 305 respectively. The third sliding groove 702 is a rectangular groove body, and its contour is the same as that of the sliding protrusion 307 mentioned above. The contours are adapted to each other, so the two can slide between them. The fixed protrusion 703 is fixedly connected to the bottom outer wall of the fourth limit block 7 away from the side of the second segmented groove 701. The fixed protrusion 703 is a plastic cuboid. The contour of the fixed protrusion 703 is adapted to the inner wall contour of the first sliding groove 202, so the fixed protrusion 703 can slide on the inner wall of the first sliding groove 202. One end of the spring 704 is fixedly connected to the outer wall of the side of the fixed protrusion 703 close to the second segmented groove 701, and the other end of the spring 704 is fixedly connected to the inner wall of the first sliding groove 202 close to the second sliding groove 204. The spring 704 is disclosed in the prior art, so it will not be described in detail. When the spring 704 is subjected to force, it will deform along the direction of its bending.

[0061] The first circular hole 207 is opened on the inner wall of the first sliding groove 202 near the center position of the second sliding groove 204. The first circular hole 207 is a circular groove body. The first circular hole 207 passes through the second sliding groove 204, so that the first sliding groove 202 is connected to the second sliding groove 204 through the first circular hole 207. The second cable 705 is fixedly connected to the outer wall of the fixed protrusion 703 near the center position of the spring 704. The second cable 705 is disclosed in the prior art, so it is not described in detail. The other end of the second cable 705 first passes through the first circular hole 207, and then passes through the second circular hole 503, and then passes through the first circular hole 207 opened on the inner wall of another first sliding groove 202, and finally is fixedly connected to the inner wall of another fixed protrusion 703 near the second sliding groove 204. When the sliding plate 502 moves along the inner wall of the second sliding groove 204 away from the support frame When the lever 202 is in the direction of rotation, the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the spring 704 will be pressed against the support frame 205, and the

[0062] The workflow of the technical solution provided by the present invention is as follows:

[0063] During the experiment, the experimenter first snaps the anesthesia mask onto the support frame 205. Next, the anesthetized mouse is gently placed on the experimental base 2, ensuring that its head is aligned with the anesthesia mask. The mouse's limbs are then placed on the top outer walls of the four C-shaped fixing plates 403. At this point, the experimenter inserts the stop pins 6 into the corresponding stop holes 408, depending on the thickness of the mouse's limbs, to secure the mouse's limbs.

[0064] Then, continue pressing the limit pin 6. Under the action of the limit clamping plate 602, the fixing plate 4 on the first clamping slot 201 moves toward the bottom of the first clamping slot 201, while the fixing plate 4 on the second clamping slot 203 moves toward the bottom of the second clamping slot 203. During this process, the S-shaped elastic plate 401 is affected by the pressing force and deforms along its own bending direction, causing the fixed mouse limbs to move with the fixing plate 4, and then driving the mouse torso to move with it until its torso is in contact with the top outer wall of the experimental base 2. At this time, stop pressing the limit pin 6 and rotate the limit pin 6 90 degrees so that it is perpendicular to the fixing plate 4. Since the length of the limiting snap-in plate 602 is adapted to the width of the bottom groove body of the first snap-in groove 201, after the experimenter releases the hand pressing the limiting pin 6, the S-shaped elastic plate 401 loses the external force and relies on its own elastic recovery deformation to push the fixed plate 4 to move toward the top of the experimental base 2. The limiting snap-in plate 602 perpendicular to the fixed plate 4 on the first snap-in groove 201 will be stuck on the inner wall near the top of the first snap-in groove 201, and the limiting snap-in plate 602 perpendicular to the fixed plate 4 on the second snap-in groove 203 will be stuck on the inner wall near the top of the second snap-in groove 203. The two limiting snap-in plates 602 will respectively prevent the fixed plates 4 on the first snap-in groove 201 and the second snap-in groove 203 from continuing to move. At this point, the mouse's torso is attached to the top outer wall of the experimental base 2.

[0065] Next, if threaded column 206 is rotated clockwise, it rotates and pushes the headrest 5 away from the support frame 205, simultaneously driving the sliding plate 502 to slide synchronously along the inner wall of the second sliding groove 204. At this point, the inclined surfaces of the polyurethane foam triangular prisms with curved ends on the headrest 5 slowly lift the mouse's head, keeping the impacted area level through fine-tuning. Simultaneously, the anesthesia mask, which is attached to the support frame 205, is brought to the mouse's mouth and nose. If, during the fine-tuning process, the impacted area of the mouse's head is level, but there is still some distance between the mouth and nose and the anesthesia mask, this can be resolved by adjusting the attachment position of the anesthesia mask to the support frame 205. Simultaneously, the sliding plate 502 pulls the second cable 705, causing the two fixing protrusions 703 to slide along the inner wall of the first sliding groove 202 toward the second sliding groove 204. The two fourth stoppers 7 follow the fixing protrusions 703, thereby clamping and securing the mouse's torso. During this process, the spring 704 is deformed by the compressive force.

[0066] After the experiment is over, the threaded column 206 can be rotated counterclockwise. The threaded column 206 will pull the head pad 5 toward the support frame 205, driving the sliding plate 502 to slide in the opposite direction along the inner wall of the second sliding groove 204. The sliding plate 502 no longer pulls the second cable 705, and the spring 704 loses the external force. It recovers its deformation by its own elasticity, driving the fixed protrusion 703 and the fourth limit block 7 to reset. The mouse's head is also lowered by the inclined surface of the polyurethane foam triangular prism on the head pad 5. At the same time, the anesthesia mask will be away from the mouse's mouth and nose to avoid hindering the removal of the mouse for observation after the experiment. Subsequently, the limit pin 6 is rotated 90 degrees again to release the limiting effect of the limit clamping plate 602. The S-shaped elastic plate 401 recovers its deformation under its own elastic action, pushing the fixed plate 4 to move toward the top of the experimental base 2, and the mouse moves with it. At this time, pull out the limit pin 6, loosen the first cable 406, release the fixation of the mouse's limbs, and the mouse can be easily removed for observation.

[0067] The present invention encompasses any alternatives, modifications, equivalents, and solutions that fall within the spirit and scope of the present invention. Preferred embodiments of the present invention are described in detail to provide a thorough understanding of the present invention, but those skilled in the art will be able to fully understand the present invention without these detailed descriptions. Furthermore, to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components, and circuits have not been described in detail.

[0068] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A special device for constructing a brain-injured mouse model based on a cortical-controlled percussion model, characterized in that: The present invention comprises a striker base, a movable support plate is installed on the top outer wall of the striker base, an experimental base is fixedly connected to the top outer wall of the movable support plate, a support frame is fixedly connected to the top outer wall of the experimental base, a first clamping groove, a first sliding groove and a second clamping groove are symmetrically opened on both sides of the top of the experimental base, and the first sliding groove is located between the first clamping groove and the second clamping groove, and a second sliding groove is opened in the middle of the top of the experimental base; A fixing component is used to fix the mouse, and the fixing component is connected to the experimental base.

2. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 1, characterized in that: The fixing assembly includes a first limit block, a second limit block and a third limit block which are symmetrically fixedly connected to both sides of the outer wall of the top of the experimental base, wherein the first limit block and the second limit block are respectively located on both sides of the first clamping groove, and the third limit block is located on one side of the second clamping groove.

3. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 2, characterized in that: The first limit block is provided with several first segmented grooves on the outer wall close to the second sliding groove, the first limit block is provided with a first avoidance groove on the outer wall close to the first clamping groove, the second limit block is provided with a second avoidance groove on the outer wall close to the first clamping groove, and the third limit block is provided with a third avoidance groove on the outer wall close to the second clamping groove.

4. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 1, characterized in that: An S-shaped elastic plate is fixedly connected to the bottom inner wall of the first clamping slot and the second clamping slot respectively. The S-shaped elastic plate is distributed in a mirror image. One end of the S-shaped elastic plate is fixedly connected to a fixed plate. Several limiting holes are opened in the middle of the top of the fixed plate.

5. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 4, characterized in that: A support plate is fixedly connected to one side of the outer wall at the top of the fixing plate, and a C-shaped fixing plate is fixedly connected to one end of the support plate. A first pull cable is fixedly connected to the outer wall of the support plate close to the C-shaped fixing plate. The C-shaped fixing plate is provided with a fourth avoidance groove running through both ends thereof, and the fourth avoidance groove is fixedly connected to the same fixing ring on both sides of the inner walls close to the bottom of the C-shaped fixing plate.

6. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 5, characterized in that: One end of the first cable is fixedly connected to a rotating cylinder, a limit pin is rotatably connected to the inner wall of the rotating cylinder, a fixed circular plate is fixedly connected to the outer wall of the limit pin near the top of the rotating cylinder, a limit clamping plate is fixedly connected to the outer wall of the limit pin near the top of the rotating cylinder, and an indicator arrow is provided on the top outer wall of the fixed circular plate.

7. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 1, characterized in that: A sliding plate is slidably connected to the inner wall of the second sliding groove, a second circular hole is opened on the outer wall of one end of the sliding plate, a head pad is fixedly connected to the end of the top of the sliding plate away from the second circular hole, and a rotating groove is opened on the outer wall of the head pad on the side close to the support frame.

8. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 1, characterized in that: A fourth limit block is slidably connected to the inner wall of the first sliding groove, and a plurality of second segmented grooves are opened on the outer wall of the fourth limit block close to the second sliding groove. The fourth limit block is symmetrically provided with third sliding grooves on the outer walls on both sides close to the second limit block and the third limit block.

9. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 8, characterized in that: A fixing protrusion is fixedly connected to the bottom outer wall of the fourth limit block, and a spring is fixedly connected to the outer wall of the fixing protrusion close to the second sliding groove, wherein the other end of the spring is fixedly connected to the inner wall of the first sliding groove close to the second sliding groove.

10. The device for constructing a brain-injured mouse model based on cortical control of blows according to claim 9, characterized in that: A first circular hole is formed on the inner wall of the first sliding groove close to the second sliding groove, and a second cable is fixedly connected to the outer wall of the fixing protrusion close to the spring. The second cable passes through the first circular hole and the second circular hole in sequence.

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