Multi-channel SRB anaerobic reaction device

Abstract

The invention relates to a multi-channel SRB anaerobic reaction device which comprises at least two independent water inlet sub-bins, an independent reaction bin is arranged corresponding to any water inlet sub-bin, and all the water inlet sub-bins are matched with one water inlet bin. According to the method, one-in-multiple-out is realized, parallel analysis of test results is facilitated, and the efficiency of screening and enriching target SRB flora and screening a carbon source material is improved; a passive operation structure is integrally formed, so that the operation and maintenance cost of the test device is reduced, and stable reaction conditions are kept; an experimenter can conveniently observe the air pressure difference in each filler bin and timely collect, detect and discharge gas generated by microorganisms, so that unstable air pressure in the sealing bin caused by accumulation of gas production amount in the filler bin is prevented; the device is provided with a detachable cover plate, so that the device can be switched to be used for an aerobic reaction test according to the requirements of other types of tests; the space is effectively saved, and by reasonably setting the height of the liquid discharging pipe, the phenomenon of short flow or local water stagnation in the water inlet bin is prevented.

Description

Technical Field

[0001] The present invention relates to the technical field of water, wastewater, sewage or sludge treatment, and in particular to a multi-channel SRB anaerobic reaction device. Background Art

[0002] Acid mine drainage (AMD) generated during sulfur-containing mining operations is typically highly acidic and contains high concentrations of heavy metal and sulfate ions. Inadequate management and treatment can lead to persistent and widespread environmental pollution and risks to watershed ecosystems. Among the various end-of-pipe AMD treatment technologies, microbial methods based on sulfate-reducing bacteria (SRB) have emerged as a highly promising passive AMD treatment technology due to their low operating and maintenance costs, environmental friendliness, and synergistic treatment effects on sulfate and heavy metals.

[0003] As an obligate anaerobic bacterium, SRB can use carbon source as electron donor and energy source to reduce sulfate to sulfide and utilize S 2- The sulfide precipitates with extremely low solubility form metal sulfides with heavy metal ions, effectively removing sulfate and heavy metals from AMD. Therefore, it is urgent to screen microbial communities with high acid and metal tolerance, identify the carbon sources that the microbial communities are good at utilizing, and design SRB anaerobic reactors with stable hydraulic conditions.

[0004] In the research field of microbial methods for reducing sulfate using SRB as the main agent, a large amount of experimental data involving different materials and processes has been accumulated. However, most studies are separate evaluations of the treatment performance of certain materials. In fact, the wastewater types, SRB flora, carbon sources and experimental conditions used in different studies vary greatly, making it difficult to directly compare the treatment effects of materials under the same conditions. In particular, in existing conventional SRB treatment process studies, it is rare to simultaneously explore the effects of multiple carbon sources on SRB removal of sulfate and heavy metals and conduct comparative analysis. This also makes the engineering design of AMD passive treatment processes lack efficient and accurate theoretical support. Summary of the Invention

[0005] The present invention solves the problems existing in the prior art and provides a multi-channel SRB anaerobic reaction device, which can treat the same AMD under the same reaction conditions for the same SRB flora using carbon sources of different material types, meeting the needs of horizontal analysis and evaluation of the impact of different carbon sources on the treatment performance of SRB flora, greatly improving the screening and enrichment efficiency of SRB flora, helping to select carbon source materials with better performance, clarifying the operating parameters of the treatment device, and providing more efficient and accurate support for the engineering design of AMD passive treatment process.

[0006] The technical solution adopted by the present invention is a multi-channel SRB anaerobic reaction device, which includes at least two independent water inlet compartments, each of which is provided with an independent reaction compartment, and all the water inlet compartments are arranged in conjunction with one water inlet compartment.

[0007] Preferably, all the water inlet compartments are evenly distributed at the lower part of the water inlet compartment, and a first adjustable valve is provided between the water inlet compartment and the water inlet compartment; a first cover plate is provided in conjunction with any water inlet compartment, and a first exhaust pipe is provided on the first cover plate.

[0008] Preferably, any of the water inlet compartments is connected to the bottom space of the corresponding reaction compartment; an auxiliary reaction component is provided at the bottom of the reaction compartment, and a reaction filler is provided on the auxiliary reaction component; a second cover plate is provided in conjunction with any of the reaction compartments, and a second exhaust pipe is provided on the second cover plate.

[0009] Preferably, a plurality of biological suspension balls are provided in the reaction chamber, and the reaction filler comprises a carbon source material filled in the biological suspension balls.

[0010] Preferably, the auxiliary reaction assembly includes a perforated plate with evenly distributed through holes.

[0011] Preferably, a gauze layer is provided between the auxiliary reaction component and the reaction filler.

[0012] Preferably, an air bag is provided in conjunction with any of the second exhaust pipes; and a first online monitoring device is provided in conjunction with the air bag.

[0013] Preferably, a drain pipe is provided on the upper portion of any reaction chamber, and a second adjustable valve is provided on the drain pipe; and a second online monitoring device is provided on the drain pipe.

[0014] Preferably, a water inlet pipe and an air inlet pipe are provided on the top of the water inlet bin.

[0015] Preferably, the water inlet bin, all the water inlet bins, and all the reaction bins are coaxially arranged.

[0016] The present invention relates to a multi-channel SRB anaerobic reaction device, comprising at least two independent water inlet compartments, each of which is provided with an independent reaction compartment, and all the water inlet compartments are arranged in conjunction with one water inlet compartment.

[0017] The beneficial effects of the present invention are: (1) The unified water inlet can flow through multiple reaction media at the same time and remove sulfate and heavy metals from the water body. The four treated water samples collected can be tested, which can achieve "one in, multiple out" and then simultaneously compare the treatment capabilities of different reaction media under the same external conditions. This is conducive to parallel analysis of test results and improves the efficiency of screening and enriching target SRB bacteria and screening carbon source materials; (2) The height difference between the water inlet compartment and the filler compartment is used to automatically form a stable water pressure, preventing the filler from excessively settling at the bottom and causing poor water distribution. The porous baffle is used to achieve uniform water distribution. At the same time, the water flow residence time in the four filler compartments is maintained consistent, forming a "passive" operation structure as a whole, which is conducive to reducing the operation and maintenance costs of the test device and maintaining stable reaction conditions. (3) The air bag device can facilitate the experimenters to observe the difference in air pressure in each filling chamber, and timely collect, detect and discharge the gas produced by microorganisms, so as to prevent the accumulation of gas production in the filling chamber from causing unstable air pressure in the sealed chamber; (4) The cover plate is detachable and can be switched to aerobic reaction test according to the needs of other types of tests; (5) It effectively saves space and prevents short-flow or local water stagnation in the water inlet tank by reasonably setting the height of the drainage pipe. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of the present invention, and both the first cover plate and the second cover plate are provided with parts; Figure 2 This is a schematic diagram of the three-dimensional structure of the present invention from a top view angle; Figure 3 It is a schematic diagram of the three-dimensional structure of the present invention from an upward viewing angle. DETAILED DESCRIPTION

[0019] The present invention is further described in detail below with reference to the embodiments, but the protection scope of the present invention is not limited thereto.

[0020] The present invention relates to a multi-channel SRB anaerobic reaction device, which includes at least two independent water inlet compartments 1. An independent reaction compartment 2 is provided corresponding to each water inlet compartment 1. All the water inlet compartments 1 are equipped with a water inlet compartment 3.

[0021] The present invention is a multi-channel anaerobic reactor test device based on a "passive" water flow mode. It provides experimental conditions for screening bacterial communities that can efficiently remove pollutants and easily utilized carbon sources for the design of acid mine wastewater treatment processes based on a microbial method dominated by SRB; each reaction chamber 2 is filled with a variety of naturally degradable slow-release carbon source materials that are easily obtained in daily life. By controlling water flow conditions and reasonable spatial layout, the microbial reaction environment under engineering implementation conditions is simulated to screen out SRB bacterial communities that can efficiently remove sulfates and metal ions and low-cost carbon sources with long service life and conducive to SRB growth, and through debugging, the reactor operating parameters with stable operating conditions are obtained to provide support for the next step of engineering application.

[0022] Considering that one of the important features of the present invention is to effectively save space, in this embodiment, the water inlet bin 3, all the water inlet bins 1, and all the reaction bins 2 are coaxially arranged, and the water inlet bin 3, all the water inlet bins 1, and all the reaction bins 2 are cylindrical, such as cylindrical; In the specific implementation process, the inner diameter of the water inlet bin 3 is set to 10 cm and the height is 10 cm. A surround-type water inlet compartment 1 is set outside it, which is divided into four water inlet compartments 1 with a width of 4 cm and a height of 70 cm. A surround-type reaction bin 2 is set outside the four water inlet compartments 1, which is divided into four sub-compartments with a width of 20 cm and a height of 45 cm. It should be noted that the bottoms of the water inlet compartment 1 and the reaction compartment 2 are flush, and the lower part of the water inlet bin 3 and the upper part of the water inlet compartment 1 partially overlap in the vertical direction; In application, the volumes of the several independent water inlet chambers 1 are equal, and the same applies to the four reaction chambers 2.

[0023] In the present invention, supporting facilities are set for different silos to achieve connectivity and isolation between the silos, including but not limited to hoses, valves, backflow prevention devices, sealing gaskets and covers, etc.; it also includes a set of equipment, namely a conductivity meter, an ORP online analyzer and a pH online analyzer, etc., which are used to realize real-time online analysis of the test device. This is content that is easy to understand for those skilled in the art, and those skilled in the art can set it up according to their needs.

[0024] All the water inlet compartments 1 are evenly distributed at the bottom of the water inlet compartment 3, and a first adjustable valve 4 is provided between the water inlet compartment 3 and the water inlet compartment 1; a first cover plate 5 is provided in conjunction with any water inlet compartment 1, and a first exhaust pipe 6 is provided on the first cover plate 5.

[0025] In the present invention, the liquid output from the water inlet bin 3 to each water inlet bin 1 is controlled by an independent first adjustable valve 4; a seal is provided at the connection between the first cover plate 5 and the water inlet bin 1 to ensure that it is suitable for an anaerobic environment, and can also be opened to achieve aerobic treatment.

[0026] Any of the water inlet compartments 1 is connected to the bottom space of the corresponding reaction compartment 2; an auxiliary reaction component is provided at the bottom of the reaction compartment 2, and a reaction filler (not shown in the figure) is provided on the auxiliary reaction component; a second cover plate 7 is provided in conjunction with any of the reaction compartments 2, and a second exhaust pipe 8 is provided on the second cover plate 7.

[0027] A plurality of biological suspension balls (not shown in the figure) are arranged in the reaction chamber 2, and the reaction filler includes a carbon source material filled in the biological suspension balls.

[0028] The auxiliary reaction component includes a perforated plate 9 with evenly distributed through holes.

[0029] A gauze layer 10 is provided between the auxiliary reaction component and the reaction filler.

[0030] In the present invention, the bottom of the water inlet compartment 1 is connected to the corresponding reaction compartment 2, and the liquid entering the water inlet compartment 1 can gradually enter the reaction compartment 2 and contact with the reaction filler to be processed.

[0031] The original intention of the design of this invention was to explore the differences in the efficiency of SRB in removing sulfate and heavy metals using different carbon sources, and to promptly collect the H2S gas produced by the SRB, maintain the stable air pressure in the reaction chamber 2, and prevent the risk of gas escape. In this embodiment, the carbon source materials in the four reaction chambers 2 are corn cobs, used mushroom culture medium, sawdust culture medium, and a culture medium uniformly mixed with the above three fillers. To prevent loss, each type of carbon source is filled in a biological suspension ball (biofilm carrier ball). Immersion experiments show that the water content of the carbon source soaked in water is approximately 45-50%.

[0032] In the present invention, in order to allow the liquid entering the reaction chamber 2 to more fully contact the carbon source material, the liquid is better dispersed through the perforated plate 9 to achieve uniform water distribution in the reaction chamber 2, and the gauze layer 10 is used to prevent the filler in the reaction chamber 2 from passing through the perforated plate 9 and settling into the water inlet chamber 1, thereby preventing the filler from being lost.

[0033] In the present invention, the configuration of the second cover plate 7 is similar to that of the first cover plate 5 .

[0034] An air bag (not shown in the figure) is provided in conjunction with any of the second exhaust pipes 8 ; a first online monitoring device (not shown in the figure) is provided in conjunction with the air bag.

[0035] In the present invention, in order to create a good anaerobic environment in the reaction chamber 2 and collect the generated gas in time, the second exhaust pipe 8 on the second cover plate 7 of each reaction chamber 2 is connected to the air bag.

[0036] A drain pipe 11 is provided on the upper portion of any reaction chamber 2 , and a second adjustable valve 12 is provided on the drain pipe 11 ; a second online monitoring device (not shown in the figure) is provided on the drain pipe 11 .

[0037] In the present invention, a flow meter is connected to the liquid discharge pipe 11 to record the actual flow rate in each reaction chamber 2 .

[0038] In the present invention, in order to achieve precise control and optimization of the operation process of the reaction system, a first online monitoring device and a second online monitoring device are respectively installed at the gas production end (the second exhaust pipe 8 and the air bag) and the water outlet (the liquid discharge pipe 11) of the system. The system operation status is controlled based on the concentration of the gas reaction product (hydrogen sulfide) and the water quality (sulfate) obtained by monitoring; Specifically, the first online monitoring device at the gas production end is a gas concentration online sensor, which can detect the concentration of hydrogen sulfide gas generated in the reaction chamber 2 in real time and judge the proliferation of the microbial community in the reaction chamber 2 and the degree of sulfate conversion into hydrogen sulfide based on the concentration. According to the changes in hydrogen sulfide gas concentration, the data is transmitted to the host computer, and the flow rate at the water inlet is dynamically adjusted through a preset control program to adapt to the changes in the metabolic activity of the microorganisms within the system and the needs of the reaction process. The second online monitoring device installed at the outlet is a TDS (total dissolved solids) sensor, which can monitor the water quality of the equipment's effluent in real time. Based on the pre-established relationship between TDS and sulfate concentration, the TDS concentration is used to indirectly characterize the sulfate concentration in the effluent. As an important indicator of the operating effect of the reaction system, sulfate concentration can directly reflect the progress of key reaction processes such as sulfate reduction within the system. Based on the sulfate concentration in the effluent and the required sulfate discharge concentration, the actual operating effect after flow adjustment is verified, and the flow control strategy is further optimized based on the water quality monitoring data to ensure that the system achieves the best treatment effect under efficient and stable dynamic operation.

[0039] In summary, the present invention cooperates with the first online monitoring device and the second online monitoring device to provide support for improving the efficiency of strain and carbon source screening and optimizing the water flow control conditions of the reactor by utilizing this microbial reaction system through an operating mode based on multi-parameter real-time monitoring and feedback control.

[0040] A water inlet pipe 13 and an air inlet pipe 14 are provided on the top of the water inlet bin 3 .

[0041] In the present invention, AMD is introduced through the water inlet pipe 13; and air is introduced through the air inlet pipe 14. In the experiment of SRB reduction of sulfate, an inert gas air inlet pipe is preset to ensure an anaerobic environment in the reaction chamber 2. Conversely, the reaction device can also be used in scenarios where an aerobic environment needs to be ensured. In this case, oxygen or air can be input through the air inlet pipe 14. Furthermore, it can also be connected to air to balance the air pressure in the reaction chamber 2. In actual applications, whether to retain the air inlet pipe 14 needs to be selected according to actual needs.

[0042] In the present invention, a viewing hole 15 may be further provided on the side wall of the reaction chamber 2 for easy observation.

[0043] The working principle of the present invention is as follows: The water flows from top to bottom, passing through the water inlet chamber 3, the water inlet chamber 1 and the reaction chamber 2 in sequence; The water inlet tank 3 is used to temporarily store the wastewater to be treated flowing in from upstream, and then the first adjustable valve 4 at the bottom of the water inlet tank 3 distributes the water flow to one or more water inlet tanks 1 (the flow rate is determined by the opening and closing degree of the first adjustable valve 4); The water flowing to the bottom of the water inlet compartment 1 enters the bottom of the corresponding reaction compartment 2 through the connecting space. Under the action of water pressure, the water flows upward by gravity (upflow) and enters the reaction compartment 2 through the orifice plate 9 of the auxiliary reaction component; The reaction chamber 2 stores nutrients to be utilized by microorganisms, and the growth and enrichment of microorganisms also occur in the reaction chamber 2. The air bag on the second cover plate 7 is used to observe and adjust the air pressure in the reaction chamber 2. When excessive gas accumulates in the chamber, it is used to exhaust gas to adjust and stabilize the air pressure in the chamber. If gas accumulation does not continue, the gas production in the reaction chamber 2 is determined by observing the size of the air bag, and thus the growth of microorganisms. In actual application, the hydrogen sulfide gas concentration in the reaction chamber 2 is also obtained based on the output of the first online monitoring device, and the flow rate at the water inlet is dynamically controlled. By setting the flow rate, the reaction time of the water flow in the reaction chamber 2 is close to the set hydraulic retention time. Then the water flows out from the discharge pipe 11, and the actual flow rate is recorded by a flow meter. The reaction progress in the reaction chamber 2 is obtained through the parameters of the second online monitoring device, and the growth of microorganisms in the reaction chamber 2 is judged. The flow control strategy is further optimized to ensure that the system achieves the best treatment effect under an efficient and stable dynamic operation state.

[0044] The present invention can be applied to the study of growth conditions of different anaerobic bacteria and aerobic bacteria by changing the size, aeration, etc.

[0045] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0046] Obviously, those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if such changes and modifications fall within the scope of the claims and their equivalents, the present invention is intended to include such changes and modifications.

Claims

1. A multi-channel SRB anaerobic reaction device, characterized by: The device comprises at least two independent water inlet compartments, each of which is provided with an independent reaction compartment, and all of the water inlet compartments are arranged in conjunction with one water inlet compartment.

2. A multi-channel SRB anaerobic reaction device according to claim 1, characterized in that: All the water inlet compartments are evenly distributed at the lower part of the water inlet compartment, and a first adjustable valve is provided between the water inlet compartment and the water inlet compartments; a first cover plate is provided to cooperate with any water inlet compartment, and a first exhaust pipe is provided on the first cover plate.

3. The multi-channel SRB anaerobic reaction device according to claim 1, characterized in that: Any of the water inlet compartments is connected to the bottom space of the corresponding reaction compartment; an auxiliary reaction component is provided at the bottom of the reaction compartment, and a reaction filler is provided on the auxiliary reaction component; a second cover plate is provided in conjunction with any of the reaction compartments, and a second exhaust pipe is provided on the second cover plate.

4. The multi-channel SRB anaerobic reaction device according to claim 3, characterized in that: A plurality of biological suspension balls are arranged in the reaction chamber, and the reaction filler comprises a carbon source material filled in the biological suspension balls.

5. The multi-channel SRB anaerobic reaction device according to claim 3, characterized in that: The auxiliary reaction component includes a perforated plate with through holes evenly distributed thereon.

6. The multi-channel SRB anaerobic reaction device according to claim 3, characterized in that: A gauze layer is provided between the auxiliary reaction component and the reaction filler.

7. The multi-channel SRB anaerobic reaction device according to claim 3, characterized in that: An air bag is provided in conjunction with any of the second exhaust pipes; and a first online monitoring device is provided in conjunction with the air bag.

8. A multi-channel SRB anaerobic reaction device according to claim 1 or 3, characterized in that: A liquid discharge pipe is provided on the upper portion of any reaction chamber, and a second adjustable valve is provided on the liquid discharge pipe; and a second online monitoring device is provided on the liquid discharge pipe.

9. The multi-channel SRB anaerobic reaction device according to claim 1, characterized in that: A water inlet pipe and an air inlet pipe are provided on the top of the water inlet bin.

10. The multi-channel SRB anaerobic reaction device according to claim 1, characterized in that: The water inlet bin, all the water inlet bins, and all the reaction bins are coaxially arranged.

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