Filtering, sorting and enriching device

Abstract

The invention belongs to the technical field of biological sample treatment, and discloses a filtering, sorting and enriching device which comprises a resuspension mechanism, a filtering and sorting mechanism and a filter membrane assembly. The resuspension mechanism can contain a liquid sample or resuspension liquid; the filtering and sorting mechanism is detachably connected to the resuspension mechanism; the filter membrane assembly is arranged in the filtering and sorting mechanism and is provided with a plurality of filter holes, a liquid sample enters the filter holes from the enrichment side of the filter membrane assembly, liquid in the liquid sample flows out of the filter holes from the filtering side of the filter membrane assembly, and target cells in the liquid sample are enriched in the enrichment side of the filter membrane assembly and / or the filter holes; and resuspending the resuspension to obtain a cell suspension. According to the filtering, sorting and enriching device, the yield and purity of target cells can be effectively improved, rare cells are prevented from being lost in the separation process, and the activity of the obtained cells is kept.

Description

Technical Field

[0001] The present invention relates to the technical field of biological sample processing, and in particular to a filtering, sorting and enrichment device. Background Art

[0002] Nucleated cells (malignant tumor cells, blood disease cells, exfoliated cardiovascular cells, maternal fetal cells, and microbial cells) in body fluids (blood, urine, pleural effusions, cerebrospinal fluid, joint effusions, saliva, sputum, and secretions, etc.) contain rare cells such as circulating tumor cells and immune cells. These rare cells have important clinical value in medicine. Currently, the detection methods for rare cells in blood can be roughly divided into two categories: one is based on the detection of rare cell surface molecular markers, and the other is based on the isolation and detection of the rare cells themselves based on their physical properties.

[0003] To separate rare cells, the first step is to isolate the rare cells from the blood and then coat them on a glass slide to create a smear. However, existing devices for filtration sorting and enriching rare cells have the problem of rare cell residue, meaning some rare cells are lost during the separation process.

[0004] Therefore, there is an urgent need for a filtering, sorting and enrichment device to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to provide a filtration sorting and enrichment device that can effectively improve the yield and purity of target cells in the filtration sorting and enrichment device, prevent the loss of rare cells during the separation process, and maintain the activity of the obtained cells.

[0006] To achieve this object, the present invention adopts the following technical solutions:

[0007] Filtration, sorting and enrichment device, including:

[0008] a resuspension mechanism, wherein the resuspension mechanism can accommodate a liquid sample or a resuspension solution;

[0009] A filtering and sorting mechanism, wherein the filtering and sorting mechanism is detachably connected to the resuspending mechanism;

[0010] The filter membrane assembly is arranged in the filtration and sorting mechanism and has multiple filter holes. The liquid sample enters the filter holes from the enrichment side of the filter membrane assembly, and the liquid in the liquid sample flows out of the filter holes from the filtration side of the filter membrane assembly. The target cells in the liquid sample are enriched on the enrichment side of the filter membrane assembly and / or in the filter holes, and then resuspended with the resuspension liquid to obtain a cell suspension.

[0011] As a preferred solution of the filtering, sorting and enriching device provided by the present invention, the inner diameter of the filter hole gradually increases in the direction away from the enrichment side and closer to the filtering side.

[0012] As a preferred solution of the filtration, sorting and enrichment device provided by the present invention, the filter membrane assembly includes a filter membrane body, and the plurality of filter holes are opened in the filter membrane body.

[0013] As a preferred solution of the filtration, sorting and enrichment device provided by the present invention, the filter membrane body is bent in a wave shape, which can increase the filtration area and thus improve the filtration efficiency.

[0014] As a preferred embodiment of the filtration, sorting and enrichment device provided by the present invention, the pleat height of the wavy-bent filter membrane body is 10 μm-100 μm.

[0015] As a preferred embodiment of the filtration, sorting and enrichment device provided by the present invention, the filter membrane body is made of polyimide, polycarbonate, polyethylene, polyethylene terephthalate, polyethylene naphthoate, polymethyl methacrylate, polystyrene and polydimethylsiloxane.

[0016] As a preferred embodiment of the filtration, sorting and enrichment device provided by the present invention, the cross-sectional shape of the filter holes is circular, regular hexagonal or regular octagonal.

[0017] As a preferred embodiment of the filtration, sorting and enrichment device provided by the present invention, a through hole is provided on the side of the filtration and sorting mechanism, and the through hole can allow the cell suspension to flow out.

[0018] As a preferred embodiment of the filtration, sorting and enrichment device provided by the present invention, the resuspension mechanism includes a accommodating component and a stopper, both of which are hollow structures, and both the accommodating component and the stopper can accommodate liquid samples or resuspensions; the accommodating component is detachably connected to the filtration and sorting mechanism, and the stopper is inserted into the filtration and sorting mechanism and can seal the through opening.

[0019] As a preferred solution of the filtration, sorting and enrichment device provided by the present invention, the containing assembly includes a cylindrical portion and a conical portion connected in sequence, the stop portion is arranged at an end of the conical portion away from the cylindrical portion, and the conical portion is detachably connected to the filtration and sorting mechanism; the inner diameter of the conical portion gradually decreases in the direction away from the cylindrical portion and approaching the stop portion.

[0020] Beneficial effects of the present invention:

[0021] The filtration sorting and enrichment device provided by the present invention includes a resuspension mechanism, a filtration sorting mechanism and a filter membrane assembly. The resuspension mechanism can accommodate liquid samples or resuspensions; the filtration sorting mechanism is detachably connected to the resuspension mechanism. The filter membrane assembly is arranged in the filtration sorting mechanism, and a plurality of filter holes are provided. The liquid sample enters the filter hole from the enrichment side of the filter membrane assembly, and the liquid in the liquid sample flows out of the filter hole from the filtration side of the filter membrane assembly. The target cells in the liquid sample are enriched on the enrichment side of the filter membrane assembly and / or in the filter hole, and then resuspended with the resuspension liquid to obtain a cell suspension. In other words, by arranging the filter holes to form a cone-shaped structure, it is possible to avoid the target cells on the enrichment side of the filter membrane assembly from clogging the filter holes, and during the resuspension process, the target cells enriched in the filter holes are more easily flushed out by the resuspension liquid, thereby effectively reducing the residual rate of the target cells in the filtration sorting and enrichment device, and preventing the rare cells from being lost during the separation process.

[0022] The filtration sorting and enrichment device provided by the present invention uses an integrated strategy to keep the sorted rare cells in the filtration sorting mechanism throughout the filtration, post-filtration recovery and smear processes, reducing the transfer steps for rare cells and thus reducing the loss of rare cells caused by the transfer process.

[0023] The filtration, sorting and enrichment device provided by the present invention adopts a three-dimensional corrugated filter membrane, which has a larger contact surface with the liquid than a two-dimensional flat membrane, and also increases the contact probability between cells and filter pores, thereby improving filtration efficiency and reducing filtration time.

[0024] The filtration, sorting and enrichment device provided by the present invention adopts a three-dimensional corrugated filter membrane component, so that the direction of pressure exerted on cells during the filtration process is dispersed, which is beneficial to maintaining the activity of the filtered cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 Schematic diagram of the structure of the filtration, sorting and enrichment device provided in an embodiment of the present invention;

[0026] Figure 2 Schematic diagram of the structure of the filtering and sorting mechanism provided by an embodiment of the present invention;

[0027] Figure 3 Schematic diagram of the structure of the filter membrane assembly provided by an embodiment of the present invention;

[0028] Figure 4 Schematic diagram of the filter portion of the filter membrane body provided by an embodiment of the present invention;

[0029] Figure 5 yes Figure 4 A local enlarged view of the structure marked A.

[0030] In the picture:

[0031] 100, resuspension mechanism; 110, stopper; 120, barrel; 121, first slot; 130, cone; 140, groove; 150, threaded section; 160, flap structure;

[0032] 200, filtering and sorting mechanism; 210, supporting ring; 220, through port; 230, internal thread portion; 240, bottom cavity; 250, second slot; 260, third slot;

[0033] 300, filter membrane assembly; 310, filter hole; 320, filter membrane body; 330, pressure ring. DETAILED DESCRIPTION

[0034] The present invention will be further described in detail below with reference to the accompanying drawings and examples. It will be understood that the specific embodiments described herein are intended only to illustrate the present invention and are not intended to limit the present invention. It should also be noted that, for ease of description, the accompanying drawings only illustrate portions relevant to the present invention, not all structures.

[0035] In the description of the present invention, unless otherwise expressly specified or limited, the terms "connected," "connected," and "fixed" should be understood in a broad sense. For example, they may refer to fixed connections, detachable connections, or integration; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal communication between two components or interaction between two components. Those skilled in the art will understand the specific meanings of the above terms in the present invention in specific circumstances.

[0036] In the present invention, unless otherwise expressly specified or limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features being in contact not directly but through another feature between them. Furthermore, a first feature being "above," "above," and "above" a second feature may include the first feature being directly above or obliquely above the second feature, or may simply mean that the first feature is higher in level than the second feature. A first feature being "below," "below," and "below" a second feature may include the first feature being directly below or obliquely below the second feature, or may simply mean that the first feature is lower in level than the second feature.

[0037] In the description of this embodiment, terms such as "upper," "lower," "right," and "left" are used to refer to positions or locations based on those shown in the accompanying drawings. These terms are intended solely to facilitate description and simplify operation, and are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation. Therefore, they should not be construed as limitations on the present invention. Furthermore, the terms "first" and "second" are used solely for descriptive purposes and have no special meaning.

[0038] This embodiment provides a filtration, sorting, and enrichment device that, with the assistance of a filter (not shown) and a smear device (not shown), can filter, sort, enrich, resuspend, and smear target cells from a liquid sample. In this embodiment, the liquid sample is a liquid biological sample (e.g., human body fluid).

[0039] Alternatively, in this embodiment, the filter can be a syringe filter, comprising a filter body (not shown) and a push-pull mechanism (not shown). The filter body is a syringe, and the push-pull mechanism is positioned above the filter body. A portion of the filter sorting and enrichment device can be connected to the push-pull mechanism, and another portion of the filter sorting and enrichment device can be driven by the push-pull mechanism to move within the filter body along its axis, thereby achieving filter sorting and enrichment of target cells.

[0040] Alternatively, in other embodiments, the filter may be a centrifugal filter comprising a filter body (not shown) and a rotary drive device (not shown). The filter body is a centrifuge tube. A portion of the filtration, sorting, and enrichment device can be connected to the rotary drive device, and another portion of the filtration, sorting, and enrichment device can be driven by the rotary drive device to rotate within the filter body, thereby achieving filtration, sorting, and enrichment of target cells through the action of centrifugal force.

[0041] Alternatively, in this embodiment, the smear device includes a receiving chamber (not shown), a smear channel (not shown), and a glass slide (not shown). The smear channel communicates between the receiving chamber and the glass slide. The filtration, sorting, and enrichment device performs a resuspension process in the receiving chamber to form a cell suspension. Then, under the action of centrifugal force, the cell suspension is guided from the filtration, sorting, and enrichment device through the smear channel to the glass slide to complete the smear process.

[0042] Figure 1 A schematic structural diagram of a filtration, sorting and enrichment device provided in an embodiment of the present invention is shown; Figure 2 A schematic structural diagram of a filtering and sorting mechanism provided in an embodiment of the present invention is shown; Figure 3 The structure diagram of the filter membrane assembly provided by the embodiment of the present invention is shown; Figure 1-Figure 3 The filtration, sorting and enrichment device includes a resuspension mechanism 100, a filtration and sorting mechanism 200 and a filter membrane assembly 300.

[0043] Specifically, the resuspension mechanism 100 can accommodate a liquid sample or a resuspension solution. The filtration and sorting mechanism 200 is detachably connected to the resuspension mechanism 100. During the filtration and sorting enrichment process and the resuspension process, the filtration and sorting mechanism 200 is connected to the resuspension mechanism 100. During the smear process, the filtration and sorting mechanism 200 is separated from the resuspension mechanism 100.

[0044] More specifically, the filter membrane assembly 300 is disposed in the filtration and sorting mechanism 200 and defines a plurality of filter holes 310. During the filtration, sorting, and enrichment process, the liquid sample enters the filter holes 310 from the enrichment side of the filter membrane assembly 300, and the liquid in the liquid sample flows out of the filter holes 310 from the filtration side of the filter membrane assembly 300. Target cells in the liquid sample are enriched on the enrichment side of the filter membrane assembly 300 and / or within the filter holes 310. During the resuspension process, the resuspension solution resuspends the target cells enriched on the enrichment side of the filter membrane assembly 300 and / or within the filter holes 310 to form a cell suspension.

[0045] Reference Figure 1 and Figure 2 The filter sorting mechanism 200 is a hollow cylindrical structure with a side opening 220 for the cell suspension to flow out after the resuspension process. During the smear process, the opening 220 can connect to a smear channel, which can guide the cell suspension from the opening 220 to the slide.

[0046] Preferably, a third slot 260 is defined on the outer side of the filtration and sorting mechanism 200, corresponding to the periphery of the opening 220. A third sealing ring is embedded in the third slot 260. This third sealing ring ensures a tight seal between the opening 220 and the entrance of the smear channel, preventing leakage of the cell suspension at the junction of the opening 220 and the smear channel during the smear process, thereby avoiding loss of target cells. In this embodiment, the third sealing ring may be a silicone seal.

[0047] Specifically, a second retaining groove 250 is provided around the outer portion of the filtration and sorting mechanism 200, and a second sealing ring is embedded in the second retaining groove 250. This second sealing ring can tightly abut the inner wall of the filter body during the filtration, sorting and enrichment process. It can also tightly abut the inner wall of the smear device's receiving chamber during the resuspension process. In this embodiment, the second sealing ring can be a silicone sealing ring.

[0048] More specifically, a support ring 210 is disposed within the inner cavity of the filtration and sorting mechanism 200. The membrane assembly 300 also includes a pressure ring 330, which presses against the membrane assembly 300, securely pressing the membrane assembly 300 against the support ring 210. This arrangement ensures reliable assembly of the membrane assembly 300 within the filtration and sorting mechanism 200.

[0049] More specifically, the bottom of the filtration and sorting mechanism 200 is provided with a bottom cavity 240, in which a magnet is provided, which is configured to adsorb non-target cells in the cell suspension. If a purification process is required after the resuspension process, an antibody-magnetic bead complex is added when the resuspension is injected into the resuspension mechanism 100. After the resuspension process is completed, the antibody-magnetic bead complex is allowed to stand for a period of time so that the non-target cells in the cell suspension that can bind to the antibody-magnetic bead complex are adsorbed under the action of the antibody-magnetic bead complex and the magnet, thereby achieving the removal of non-target cells. In this embodiment, the antibody-magnetic bead complex can be selected as CD45 antibody magnetic beads.

[0050] Continue to refer to Figure 1 The resuspension mechanism 100 includes a hollow container assembly. The container assembly includes a cylindrical portion 120 and a conical portion 130 that are connected in sequence. Both the cylindrical portion 120 and the conical portion 130 can accommodate a liquid sample or a resuspension solution. The conical portion 130 is detachably connected to the filtration and sorting mechanism 200.

[0051] Specifically, the resuspension mechanism 100 also includes a stop portion 110 with a hollow structure, which is arranged at one end of the cone portion 130 away from the cylindrical portion 120. When the cone portion 130 is connected to the filtration and sorting mechanism 200, the stop portion 110 is inserted into the filtration and sorting mechanism 200 and can block the through port 220 to prevent the liquid sample or cell suspension from flowing out of the through port 220 during the filtration and sorting enrichment process or the resuspension process.

[0052] More specifically, the re-suspension mechanism 100 further includes a threaded section 150 disposed between the stopper 110 and the conical portion 130. An internal threaded section 230 is disposed at the top opening of the filtration and sorting mechanism 200, and the threaded section 150 can be screwedly connected to the internal threaded section 230 to achieve a detachable connection between the re-suspension mechanism 100 and the filtration and sorting mechanism 200.

[0053] More specifically, the re-suspension mechanism 100 further includes a flap structure 160. The flap structure 160 is disposed at one end of the cylindrical portion 120 away from the conical portion 130, and is configured as a push-pull device connected to the filter.

[0054] More specifically, the cylindrical portion 120 is provided with a first groove 121, in which a first sealing ring is embedded. The first sealing ring can be tightly pressed against the inner wall of the filter body. In this embodiment, the first sealing ring can be a silicone sealing ring.

[0055] Preferably, the inner diameter of the cone portion 130 gradually decreases in a direction away from the barrel portion 120 and closer to the stop portion 110. This configuration facilitates the liquid sample to flow along the inner wall of the cone portion 130 toward the filtering and sorting mechanism 200.

[0056] Alternatively, a groove 140 is provided at the connection between the barrel 120 and the cone 130, so that the barrel 120 and the cone 130 can be separated from each other at the groove 140. When a cell suspension is formed and a subsequent smear process is required, the resuspending mechanism 100 and the filtration and sorting mechanism 200 connected together are first inserted into the receiving chamber of the smear device at the same time. Then, the resuspending mechanism 100 and the filtration and sorting mechanism 200 are pressed into the bottom of the receiving chamber at the same time. In this process, the cells are resuspended to form a cell suspension. Then, by twisting and separating the resuspending mechanism 100 and the filtration and sorting mechanism 200, the port 220 is connected to the smear channel, and the resuspending mechanism 100 is broken off at the groove 140 to separate the barrel 120 and the cone 130. Finally, the smear process is performed by centrifugation.

[0057] Figure 4 A schematic diagram showing a filtration portion of a filter membrane body provided by an embodiment of the present invention; Figure 5 Show Figure 4 A partial enlarged view of the structure marked as A. The filter membrane assembly 300 includes a filter membrane body 320, and a plurality of filter holes 310 are opened in the filter membrane body 320. The filter membrane body 320 includes a filter portion and a connection portion. The connection portion is integrally formed on the periphery of the filter portion, and the connection portion is clamped between the pressure ring 330 and the support ring 210. The filter portion is located in the middle hole of the pressure ring 330, and a plurality of filter holes 310 are opened in the filter portion. In this embodiment, the pore spacing between adjacent filter holes 310 is 10μm-50μm, preferably 23μm, and the above-mentioned pore spacing is the distance between the geometric centers of two adjacent filter holes 310. It should be noted that, in this embodiment, the surface of the filter membrane body 320 can be treated to be hydrophilic by providing a coating or the like.

[0058] Specifically, the inner diameter of the filter pore 310 gradually increases in the direction away from the enrichment side and closer to the filtration side. Through the above-mentioned setting, the filter pore 310 is set to form a cone-shaped structure, which can prevent the target cells from clogging the filter pore 310 on the filtration side of the filter membrane assembly 300. And during the resuspension process, it is convenient for the resuspension liquid to enter the filter pore 310 from the filtration side, and flush out the target cells enriched in the filter pore 310, thereby effectively reducing the residual rate of the target cells in the filtration sorting enrichment device, and preventing rare cells from being lost during the separation process. In this embodiment, the diameter of the maximum end of the inner diameter of the filter pore 310 is 9μm-15μm, preferably 12μm; the diameter of the minimum end of the inner diameter of the filter pore 310 is 5μm-9μm, preferably 7μm.

[0059] Preferably, in this embodiment, the cross-sectional shape of the filter hole 310 is a circle, a regular hexagon, or a regular octagon.

[0060] More specifically, the filter portion of the filter membrane body 320 is undulating. This arrangement effectively increases the filter area. Furthermore, the arc-shaped transitions of the undulating structure disperse the pressure exerted on the cells during filtration, reducing damage to the target cells and thereby improving the efficiency of filtration, sorting, and enrichment, as well as the activity of the target cells. In this embodiment, the pleats of the undulating filter membrane body 320 have a height of 10 μm to 100 μm.

[0061] More specifically, the filter membrane body 320 is made of polyimide, polycarbonate, polyethylene, polyethylene terephthalate, polyethylene naphthoate, polymethyl methacrylate, polystyrene and polydimethylsiloxane.

[0062] The process of filtering, sorting, enriching and resuspending target cells by the filtration, sorting and enrichment device provided in this embodiment is as follows:

[0063] First, process the whole blood sample. Take 5 ml of whole blood and add 5 ml of red blood cell lysis buffer in a 1:1 ratio. Mix thoroughly and let stand for 10 minutes. Then, add 1.333 ml of fixative in a 15:2 ratio. Mix thoroughly and let stand for 10 minutes. The fixative can be 4% formaldehyde solution, and the red blood cell lysis buffer consists of 0.1% KHCO₃, 0.83% NH₄Cl, and 0.037‰ EDTA-Na₂.

[0064] Perform filtration sorting and enrichment process:

[0065] First, connect the resuspension mechanism 100 and the filtration and separation mechanism 200, and install them into the filter body. Use a soft plug to block the outlet of the filter body to form a closed syringe structure.

[0066] Then, the flap structure 160 is fixed to the corresponding position of the push-pull device;

[0067] Then, 5 mL of PBS solution was added to the resuspension mechanism 100 and the filtration and sorting mechanism 200 to rinse the filter membrane 320. Then, 1 mL of PBS solution was added, and 10 mL of the treated whole blood sample was added at the same time. When the last 1 mL of whole blood sample was added, 3 mL of PBS solution was added to rinse the walls of the resuspension mechanism 100 and the filtration and sorting mechanism 200 in a shower-like manner.

[0068] Finally, a push-pull device is used to pull the resuspension mechanism 100 and the filtration and separation mechanism 200 to move at a speed of 3.12 mm / min. In this step, the push-pull device can be selected as a syringe pump, which can be connected to a power supply to achieve push-pull.

[0069] Perform the resuspension process:

[0070] After the liquid in the resuspension mechanism 100 and the filtration and sorting mechanism 200 is filtered out, add the antibody-magnetic bead complex and the resuspension solution, and press the resuspension mechanism 100 and the filtration and sorting mechanism 200 downward by 5 cm each time. Let it stand for 2 minutes, blow gently 10 times, and then transfer the cell suspension to a new tube.

[0071] The cell suspension can be used for subsequent smears and observed under a microscope after cell staining or immunostaining. It can also be used for subsequent PCR, NGS, or cell fluorescence flow cytometry analysis.

[0072] Obviously, the above embodiments of the present invention are merely examples for the purpose of clearly illustrating the present invention and are not intended to limit the embodiments of the present invention. A person skilled in the art would be able to make various obvious changes, readjustments, and substitutions without departing from the scope of protection of the present invention. It is not necessary and impossible to enumerate all embodiments here. Any modifications, equivalent substitutions, and improvements made within the spirit and principles of the present invention shall be included within the scope of protection of the claims of the present invention.

Claims

1. Filtration, sorting and enrichment device, characterized in that: include: a resuspension mechanism (100), wherein the resuspension mechanism (100) is capable of accommodating a liquid sample or a resuspension liquid; A filtering and sorting mechanism (200), wherein the filtering and sorting mechanism (200) is detachably connected to the resuspending mechanism (100); A filter membrane assembly (300) is provided in the filtering and sorting mechanism (200) and has a plurality of filter holes (310). The liquid sample enters the filter holes (310) from the enrichment side of the filter membrane assembly (300), and the liquid in the liquid sample flows out of the filter holes (310) from the filtration side of the filter membrane assembly (300). The target cells in the liquid sample are enriched on the enrichment side of the filter membrane assembly (300) and / or in the filter holes (310), and then resuspended with the resuspension liquid to obtain a cell suspension.

2. The filtration, sorting and enrichment device according to claim 1, characterized in that: The inner diameter of the filter pore (310) gradually increases in a direction away from the enrichment side and closer to the filtration side.

3. The filtration, sorting and enrichment device according to claim 1, characterized in that: The filter membrane assembly (300) includes a filter membrane body (320), and a plurality of filter holes (310) are opened in the filter membrane body (320).

4. The filtration, sorting and enrichment device according to claim 3, characterized in that: The filter membrane body (320) is bent in a wave shape.

5. The filtration, sorting and enrichment device according to claim 4, characterized in that: The fold height of the wave-shaped filter membrane body (320) is 10 μm-100 μm.

6. The filtration, sorting and enrichment device according to claim 3, characterized in that: The filter membrane body (320) is made of polyimide, polycarbonate, polyethylene, polyethylene terephthalate, polyethylene naphthoate, polymethyl methacrylate, polystyrene and polydimethylsiloxane.

7. The filtration, sorting and enrichment device according to claim 1, characterized in that: The cross-sectional shape of the filter hole (310) is circular, regular hexagonal, or regular octagonal.

8. The filtration, sorting and enrichment device according to claim 1, characterized in that: A through hole (220) is provided on the side of the filtering and sorting mechanism (200), and the through hole (220) can allow the cell suspension to flow out.

9. The filtering, sorting and enriching device according to claim 8, characterized in that: The resuspension mechanism (100) comprises a accommodating component and a stopper (110), both of which are hollow structures. The accommodating component and the stopper (110) are both capable of accommodating the liquid sample or the resuspension liquid. The accommodating component is detachably connected to the filtering and sorting mechanism (200), and the stopper (110) is inserted into the filtering and sorting mechanism (200) and is capable of blocking the through port (220).

10. The filtration, sorting and enrichment device according to claim 9, characterized in that: The accommodating assembly comprises a cylindrical portion (120) and a conical portion (130) which are connected in sequence, the stop portion (110) is arranged at one end of the conical portion (130) away from the cylindrical portion (120), and the conical portion (130) is detachably connected to the filtering and sorting mechanism (200); and the inner diameter of the conical portion (130) gradually decreases in a direction away from the cylindrical portion (120) and approaching the stop portion (110).

Images