Shampoo containing plant exosome and minoxidil and preparation method thereof

Abstract

The invention provides shampoo containing plant exosomes and minoxidil and a preparation method of the shampoo. The shampoo can effectively prevent alopecia, nourish scalp, improve hair quality and reduce dandruff by utilizing the characteristics of antioxidation, anti-inflammation, permeability increase and the like of the plant exosome. The preparation method is simple and feasible and is suitable for large-scale production.

Description

Technical Field

[0001] The present invention relates to a shampoo, in particular to a shampoo containing plant exosomes and minoxidil and a preparation method thereof, belonging to the technical field of anti-hair loss shampoo. Background Art

[0002] The accelerated pace of modern life and increased stress have led to a noticeable increase in the prevalence and prevalence of hair loss, which has a significant negative impact on social interaction and mental health. Existing daily anti-hair loss products struggle to be fully absorbed by the scalp, resulting in poor results. Furthermore, since most products contain organic solvents, long-term use can damage the scalp and hair.

[0003] Technical background: The formula of common shampoo currently contains the following components:

[0004] 1. Surfactants: responsible for cleaning, such as sodium lauryl sulfate (SLS) and cocamidopropyl betaine.

[0005] 2. Conditioning agents: increase softness and gloss, such as silicone oil and guar gum hydroxypropyltrimonium chloride.

[0006] 3. Moisturizers: maintain moisture balance, such as glycerin and hyaluronic acid.

[0007] 4. Preservatives: extend shelf life, such as phenoxyethanol and imidazolidinyl urea.

[0008] 5. pH regulator: adjust the pH, such as citric acid and sodium hydroxide.

[0009] 6. Flavors and pigments: provide fragrance and color to enhance appeal.

[0010] 7. Active ingredients: targeting specific problems, such as anti-hair loss and anti-dandruff ingredients.

[0011] 8. Thickener: Improves the feel of use, such as carbomer and sodium chloride.

[0012] 9.Solvent: Ensure the formula is uniform and stable, such as water.

[0013] Existing technology solution CN 115252647 A, a composite topical preparation for promoting hair growth, its preparation, and application: a composite topical preparation containing minoxidil loaded into exosomes. The composite preparation consists of minoxidil loaded into exosomes. The exosomes are obtained from cells in passages 3 to 9 of the logarithmic growth phase, and the minoxidil-to-exosome ratio is 1:8 to 8:1. It is used to treat androgenic alopecia, telogen effluvium, and alopecia areata.

[0014] Exosomes are tiny membrane vesicles secreted by cells, typically ranging in diameter from 30 to 150 nanometers. They play a vital role in intercellular communication, transport of substances, and signal transduction. Exosomes can carry biomolecules such as proteins, lipids, DNA, mRNA, and miRNA, and can deliver these molecules to other cells, thereby influencing the function of recipient cells. In recent years, plant exosomes have garnered widespread attention due to their excellent biocompatibility and diverse bioactive properties. Plant exosomes possess antioxidant, anti-inflammatory, and damage-repairing properties, effectively promoting scalp health and improving hair quality.

[0015] Minoxidil, also known as Minoxidil, is a medication commonly used to treat hair loss and promote hair growth. It works by dilating scalp blood vessels, increasing blood flow to hair follicles and providing more oxygen and nutrients, thereby promoting healthy hair follicle growth. It can also prolong the growth phase of hair follicles, allowing more follicles in the resting phase to re-enter the growth phase, reducing hair loss and promoting new hair growth. However, it has drawbacks such as slow onset of action, hirsutism, and a high risk of recurrence of hair loss after discontinuation. Summary of the Invention

[0016] The present invention mainly addresses the deficiencies in the prior art and provides a shampoo containing plant exosomes and minoxidil and a preparation method thereof, aiming to utilize the bioactive substances of plant exosomes to achieve the effects of nourishing the scalp and preventing hair loss.

[0017] The above technical problems of the present invention are mainly solved by the following technical solutions:

[0018] A shampoo containing plant exosomes and minoxidil, comprising the following components by weight:

[0019] Surfactant: 10-20%; plant exosomes: 5-25%; minoxidil: 0.01-1%; moisturizer: 5-10%; antioxidant: 1-3%; flavor: 0.1-0.5%; physiological saline: balance.

[0020] Preferably, the plant exosomes are from Platycladus orientalis leaves or ginger.

[0021] A preparation method containing plant exosomes and minoxidil:

[0022] (1) Preparation of plant exosomes:

[0023] Collect fresh arborvitae leaves or ginger, wash and chop them;

[0024] Add the chopped plant material into an appropriate amount of physiological saline and homogenize it;

[0025] The homogenate was centrifuged and the supernatant was taken;

[0026] The supernatant is further separated by ultrafiltration or dialysis to obtain plant exosomes;

[0027] (2) Plant exosomes coated with minoxidil:

[0028] Mix exosomes and minoxidil in proportion;

[0029] By repeatedly freezing and thawing the exosomes three times, the membrane structure was destroyed, allowing minoxidil to enter;

[0030] (3) Preparation of shampoo:

[0031] Add surfactant, moisturizer, antioxidant and fragrance into normal saline in the above weight parts in sequence and stir evenly;

[0032] Add the plant exosomes coated with minoxidil into the above mixture and continue stirring evenly;

[0033] The mixed solution is filtered and sterilized to obtain a shampoo containing plant exosomes.

[0034] Preferably, in step (1), the homogenate is centrifuged at 4°C, 10,000g, for 30 min, and the supernatant is collected.

[0035] Preferably, in step (2), the exosomes and minoxidil are mixed in a ratio of 1:100.

[0036] Preferably, in step (iii), the mixed solution is sterilized by filtering through a 0.22 μM filter membrane.

[0037] Exosome-encapsulated minoxidil is an emerging drug delivery technology that aims to improve the efficacy and targeting of minoxidil by utilizing exosomes as carriers. Specifically, exosome-encapsulated minoxidil has the following advantages:

[0038] Enhanced permeability: Exosomes have natural cell-penetrating properties and can more easily pass through the scalp stratum corneum and other biological barriers, delivering minoxidil directly into the deep of the hair follicles, thereby increasing the local concentration and absorption efficiency of the drug.

[0039] Prolonged duration of action: Since exosomes can exist stably in the body for a long time, they can continuously release the minoxidil encapsulated within them, providing a more lasting therapeutic effect.

[0040] Reduced irritation: Traditional topical minoxidil may cause adverse reactions such as scalp irritation and facial hirsutism. The exosome-encapsulated form can help alleviate these side effects, making the drug gentler and suitable for sensitive scalps.

[0041] Avoid systemic absorption: Through exosome encapsulation, minoxidil is mainly concentrated in the scalp area to exert its effect, reducing unnecessary systemic absorption and lowering potential cardiovascular risks.

[0042] This shampoo is mild and non-irritating, with rich foam. After use, the hair is smooth and flowing, and the skin is clean and comfortable. It combines hair cleansing, hair care and skin health in one.

[0043] The shampoo of the present invention utilizes the antioxidant, anti-inflammatory, and damage-repairing properties of plant exosomes to effectively nourish the scalp, improve hair quality, and reduce dandruff and hair loss. Furthermore, the preparation method of the present invention is simple and easy to implement, making it suitable for large-scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 This is a transmission electron microscope observation of the Platycladus orientalis leaf exosomes obtained in Example 1 of the present invention;

[0045] Figure 2 This is a transmission electron microscope observation diagram of Example 1 of the present invention;

[0046] Figure 3 It is a comparison chart of the usage status of recruited volunteers in the present invention. DETAILED DESCRIPTION

[0047] The technical solution of the present invention is further specifically described below through examples.

[0048] Example 1: A shampoo containing plant exosomes and minoxidil, the components of which are as follows by weight:

[0049] The surfactant is sodium lauryl sulfate: 13%;

[0050] Plant exosomes are Platycladus orientalis leaf exosomes: 12%;

[0051] Minoxidil: 0.06%;

[0052] Moisturizing agent is glycerin: 8%;

[0053] Antioxidant is vitamin C: 2%;

[0054] The fragrance is rose essential oil: 0.3%;

[0055] Normal saline: balance;

[0056] The preparation method is as follows:

[0057] Collect fresh Platycladus orientalis leaves, clean and chop them, add appropriate amount of physiological saline and homogenize them;

[0058] The homogenate was centrifuged, and the supernatant was further separated by ultrafiltration to obtain Platycladus orientalis leaf exosomes;

[0059] The exosomes obtained from Platycladus orientalis leaves were observed by transmission electron microscopy ( Figure 1 ), Results: Small vesicles with very obvious membrane boundaries and typical saucer or elliptical structures were observed under the electron microscope.

[0060] Electron microscopy can observe the morphological characteristics of exosomes, but it cannot reflect the particle size distribution and overall concentration of all exosomes in the sample. Therefore, NTA testing was performed:

[0061]

[0062] NTA test results: The feasibility of the exosome extraction method was further confirmed in terms of exosome concentration and particle size.

[0063] Platycladus orientalis leaf exosomes and minoxidil were mixed in appropriate proportions, and the exosomes were repeatedly frozen and thawed three times to destroy the membrane structure and allow minoxidil to enter;

[0064] Add sodium lauryl sulfate, glycerin, vitamin C and rose essential oil to normal saline in sequence and stir evenly;

[0065] Add the Platycladus orientalis leaf exosomes coated with minoxidil to the above mixture and continue to stir evenly; 6. Filter and sterilize the mixture to obtain a shampoo containing Platycladus orientalis leaf exosomes and minoxidil.

[0066] This product was observed under a transmission electron microscope (e.g. Figure 2 ), Results: Typical cup-shaped vesicles of exosomes were observed under an electron microscope, and no rupture occurred, indicating that the exosomes encapsulating minoxidil can be stably preserved in the product.

[0067] Recruit volunteers to use this product twice a week and test its anti-hair loss function (such as Figure 3 ), Results: As shown in the figure, after 3 months, the amount of hair increased significantly, indicating that this product has an anti-hair loss effect.

[0068] Example 2:

[0069] A shampoo containing plant exosomes and minoxidil, the components of which are as follows by weight:

[0070] Surfactant: Cocamidopropyl Hydroxysultaine: 15%

[0071] Plant exosomes are ginger exosomes: 15%

[0072] Minoxidil: 0.03%

[0073] Moisturizing agent is hyaluronic acid: 7%

[0074] Antioxidant as Vitamin E: 1%

[0075] Lemon essential oil fragrance: 0.2%

[0076] Normal saline: balance

[0077] The preparation method is similar to that of Example 1, except that the plant exosomes are replaced with ginger exosomes.

Claims

1. A shampoo containing plant exosomes and minoxidil, characterized in that By weight, it comprises the following components: Surfactant: 10-20%; Plant exosomes: 5-25%; minoxidil: 0.01-1%; moisturizer: 5-10%; antioxidant: 1-3%; flavor: 0.1-0.5%; normal saline: balance.

2. The shampoo containing plant exosomes and minoxidil according to claim 1, characterized in that: The plant exosomes are either from Platycladus or ginger.

3. A method for preparing a shampoo containing plant exosomes and minoxidil, the method using the shampoo according to any one of claims 1 to 2, characterized in that: (1) Preparation of plant exosomes: Collect fresh arborvitae leaves or ginger, wash and chop them; The chopped plant material was added to an appropriate amount of physiological saline and homogenized; The homogenate was centrifuged and the supernatant was taken; The supernatant is further separated by ultrafiltration or dialysis to obtain plant exosomes; (2) Plant exosomes coated with minoxidil: Mix exosomes and minoxidil in proportion; By repeatedly freezing and thawing the exosomes three times, the membrane structure was destroyed, allowing minoxidil to enter; (3) Preparation of shampoo: Add surfactant, moisturizer, antioxidant and fragrance into normal saline in the above weight parts in sequence and stir evenly; Add the plant exosomes coated with minoxidil into the above mixture and continue stirring evenly; The mixed solution is filtered and sterilized to obtain a shampoo containing plant exosomes.

4. The method for preparing a product containing plant exosomes and minoxidil according to claim 3, wherein: In the step (1), the homogenate is centrifuged at 4° C., 10,000 g, for 30 min, and the supernatant is collected.

5. The preparation method containing plant exosomes and minoxidil according to claim 3, characterized in that : In the step (2), the exosomes and minoxidil are mixed in a ratio of 1:

100.

6. The preparation method containing plant exosomes and minoxidil according to claim 3, characterized in that : In the step (3), the mixed solution is sterilized by filtering through a 0.22 μM filter membrane.

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