System, preparation method and application of acute lung injury model suitable for evaluating the repair effect of nano-blocking membrane

Abstract

This application relates to the field of biomedical technology and specifically discloses a system, preparation method, and application of an acute lung injury model suitable for evaluating the repair efficacy of nano-occluding membranes. This method, based on the synergistic mechanism of mechanical ventilation and thermal injury, achieves periodic dynamic stretching of lung tissue through a rapid inflation mode. Combined with the thermal effects of an electrosurgical unit, this model constructs an acute lung injury model with a dual-action coupling of "mechanical stretch and thermal injury." Compared to traditional modeling methods such as chemical or biological induction, this method can precisely control the size of lung lobe wounds and achieve standardized simulation of the pathological characteristics of traumatic alveolar rupture. This overcomes technical bottlenecks such as uncontrollable wound morphology and deviations in the localization of the injury site in traditional models, providing a highly reproducible experimental vehicle for evaluating the sealing performance and repair efficacy of nano-occluding membranes under dynamic pressure environments.

Description

Technical Field

[0001] The present application relates to the field of biomedical technology, and in particular to a system, preparation method, and application of an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. Background Art

[0002] New nano-blocking membranes demonstrate significant potential in the field of dynamic tissue repair due to their significant nanomaterial-tissue interface activity. Currently, the repair and blocking performance of these materials is often evaluated using traditional cardiac interventional surgery models. However, this approach presents challenges such as high operational risk and high costs, and it is difficult to directly verify the dynamic repair capabilities of nano-blocking membranes. However, lung injury models and heart injury models have similarly stringent requirements for biosafety, dynamic mechanical adaptability, and blocking efficiency accuracy in the verification of blocking membrane performance. Furthermore, lung tissue has higher material compliance requirements than the heart. Therefore, constructing a lung injury model can provide an important technical reference for the development of nano-blocking membranes.

[0003] Commonly used lung injury models currently include biologically induced models, physical impact models, ventilator-associated models, ischemia-reperfusion models, and hydrochloric acid infusion models. However, these models all have limitations, making it difficult to accurately verify the performance of nanoblocking membranes. For example, biologically induced models struggle with precise regulation of lung injury due to uncontrolled inflammation; physical impact models suffer from large individual variability and low animal survival rates due to the difficulty in controlling the physical damage (force magnitude and direction); ventilator-associated models are pathogen-dependent and have delayed assessments; and ischemia-reperfusion and hydrochloric acid infusion models face complex operational procedures.

[0004] Therefore, it is urgent to develop a new lung injury model that can accurately locate the injury site in order to scientifically verify the dynamic blocking effect of the nanoblocking membrane. Summary of the Invention

[0005] In view of this, the purpose of this application is to provide a system, preparation method and application of an acute lung injury model suitable for evaluating the repair effect of nano-blocking membranes, so as to solve the problem that existing lung injury models are difficult to accurately locate the injury site and cannot effectively verify the dynamic blocking effect of nano-blocking membranes.

[0006] To achieve the above technical objectives, the present application provides a system for an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane, comprising an anesthesia unit, an inflation unit, an electrocoagulation burning unit, and an air leakage testing unit;

[0007] The anesthesia unit is used to perform combined anesthesia treatment on animals through intramuscular anesthesia and respiratory anesthesia;

[0008] The inflation unit is used to perform cyclic inflation on the lung lobes of an animal in an anesthetized state so as to cause the lung lobes to produce cyclic undulating motion;

[0009] The electrocoagulation burning unit is used to form a wound surface on the cyclically undulating surface of the lung lobe;

[0010] The air leakage test unit is used to detect whether there is air leakage in the lung lobe with a wound surface.

[0011] The present application provides a method for preparing an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. The acute lung injury model is prepared using the above-mentioned system, comprising the following steps:

[0012] Step S1, performing combined intramuscular anesthesia and respiratory anesthesia on the animal, with respiratory anesthesia using a pressure-controlled mode;

[0013] Step S2: Open the animal's surface muscles to expose the lung lobes, and adjust the pressure control mode to a rapid inflation mode to allow the lung lobes to fully expand during inflation;

[0014] Step S3, lightly pressing the electrode head of the electrosurgical knife on the surface of the lung lobe to form an eschar wound on the surface of the lung lobe, and peeling off the eschar wound to obtain a lung lobe wound;

[0015] Step S4: Adjust the rapid inflation mode to the pressure control mode. When air leakage occurs in the lung wound, an acute lung injury model is obtained.

[0016] Furthermore, the peak airway pressure of the pressure control mode is set to 10-15 cmH2O; the parameters of the rapid inflation mode are set as follows: the peak airway pressure is 10-15 cmH2O; the single inflation time is 3-7 seconds; and / or the number of breathing cycles of a single inflation is 4-6 times.

[0017] Furthermore, the electric knife is equipped with a spherical electrode head with a diameter of 1 to 2 mm, and the working power of the electric knife is 30 to 100 W.

[0018] Furthermore, when the electrode head of the electrosurgical knife is lightly pressed on the surface of the lung lobe, the contact time between the electrode head and the lung lobe is 3 to 5 seconds, so as to form an eschar wound with a diameter of 3 to 5 mm.

[0019] Furthermore, the air leakage phenomenon is evaluated as follows: use a ventilator to switch the animal's breathing mode to pressure control mode, set the airway peak pressure to 10-15 cmH2O to simulate a dynamic respiratory pressure environment, cover the lung lobe wound with a medical pad, and when the maximum floating amplitude of the pad during the respiratory cycle is greater than or equal to 1 mm, it is determined that air leakage occurs in the lung lobe.

[0020] Furthermore, to determine whether the acute lung injury model was successfully established, air leakage phenomenon assessment combined with histopathological assessment was used for determination;

[0021] Among them, histopathological evaluation includes the following two evaluation methods:

[0022] Evaluation method 1: After the animal model is established, the wound surface is sutured and the animal is artificially reared. Within 3 days, the local inflammatory cells in the modeling area are semi-quantitatively scored. If the total score is greater than or equal to 9 points, the acute lung injury model is considered to be successfully established;

[0023] Evaluation method 2: After the animal modeling, the wound surface is sutured and then artificially raised. On the 28th day, the local inflammatory cell semi-quantitative score, local tissue pathomorphological score and local tissue fibrosis pathomorphological score of the modeling site are scored, and the total score is calculated at the same time; the total score = inflammatory cell semi-quantitative score × 20% + local tissue pathomorphological score × 30% + local tissue fibrosis pathomorphological score × 50%. When the total score is greater than or equal to 2 points, the acute lung injury model is considered to be successfully prepared.

[0024] The present application provides an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. The model is prepared using a preparation method for an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. There is a regular wound surface on the lung lobe that stably produces air leakage.

[0025] The present application provides an application of an acute lung injury model for evaluating the repair effect of a nano-blocking membrane.

[0026] The present application provides a method for evaluating the repair effect of a nano-occluding membrane, and uses an acute lung injury model to evaluate the repair effect of the nano-occluding membrane.

[0027] Furthermore, a method for evaluating the repair effect of the nano-occluding membrane comprises the following steps: using the nano-occluding membrane to cover the lung lobe wound of an acute lung injury model, and evaluating the repair effect of the nano-occluding membrane by observing the stability of the nano-occluding membrane.

[0028] In summary, the present application provides a method for preparing an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. This method is based on the synergistic mechanism of mechanical ventilation and thermal damage. It realizes periodic dynamic stretching of lung tissue through a rapid inflation mode, and combines the thermal effect of an electric knife to construct an acute lung injury model with a dual-action coupling of "mechanical stretching-thermal damage". Compared with traditional modeling methods such as chemical induction or biological induction, this method can accurately control the size of the lung lobe wound and realize standardized simulation of the pathological characteristics of traumatic alveolar rupture, breaking through the technical bottlenecks of traditional models such as uncontrollable wound morphology and deviation in positioning of the injury site.

[0029] The acute lung injury model provided in this application has regular wound surface characteristics and can accurately reproduce the air leakage pathological state of clinical lung injury, which can provide a standardized experimental carrier for verifying the repair performance of nano-blocking membranes.

[0030] The present application provides a method for evaluating the repair effect of a nano-blocking membrane. This method is based on an acute lung injury model and can scientifically evaluate the repair effect of the blocking membrane simply by observing the stability of the nano-blocking membrane under dynamic pressure (including interfacial adhesion, air leakage control efficiency, and mechanical adaptability). This method combines the simplicity of experimental operation with the reliability of the results. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0032] Figure 1 A schematic diagram of the dynamic fluctuations of the lung lobe and the towel on the lung lobe wound surface provided in an embodiment of the present application;

[0033] Figure 2 Schematic diagram of lung lobe wounds created by electrosurgical units of different powers provided in the embodiments of the present application;

[0034] Figure 3 Schematic diagram of HE staining and Masson staining of lung lobe wounds under different power electrocautery provided in the examples of this application on the 28th day. DETAILED DESCRIPTION

[0035] The following will clearly and completely describe the technical solutions of the embodiments of this application in conjunction with the accompanying drawings. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this specification, all other embodiments obtained by ordinary technicians in this field without making any creative efforts are within the scope of protection requested by this application.

[0036] The sources of all raw materials in the present invention are not particularly limited and can be purchased on the market or prepared according to conventional methods known to those skilled in the art.

[0037] The embodiment of the present application provides a system for an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane, comprising an anesthesia unit, an inflation unit, an electrocoagulation burning unit, and an air leakage testing unit;

[0038] The anesthesia unit is used to administer combined anesthesia to animals through intramuscular anesthesia and respiratory anesthesia;

[0039] The inflation unit is used to cyclically inflate the lung lobes of an animal in an anesthetized state to produce cyclic undulating motion of the lung lobes;

[0040] The electrocoagulation and cauterization unit is used to form wounds on the circulatory and undulating surface of the lung lobe;

[0041] The air leakage test unit is used to detect whether there is air leakage in the lung lobe with a wound surface.

[0042] The present invention provides a method for preparing an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. The acute lung injury model is prepared using the above-mentioned system, comprising the following steps:

[0043] Step S1, performing combined intramuscular anesthesia and respiratory anesthesia on the animal, with respiratory anesthesia using a pressure-controlled mode;

[0044] Step S2: Open the animal's surface muscles to expose the lung lobes, and adjust the pressure control mode to a rapid inflation mode to allow the lung lobes to fully expand during inflation;

[0045] Step S3, lightly pressing the electrode head of the electrosurgical knife on the surface of the lung lobe to form an eschar wound on the surface of the lung lobe, and peeling off the eschar wound to obtain a lung lobe wound;

[0046] Step S4: Adjust the rapid inflation mode to the pressure control mode. When air leakage occurs in the lung wound, an acute lung injury model is obtained.

[0047] It should be noted that this example uses a rapid inflation mode to dynamically expand lung tissue, combined with the thermal damage effect of an electric scalpel to create a uniformly sized, regular wound on the surface of the lung lobe, thereby simulating the pathological characteristics of traumatic alveolar rupture and successfully constructing an acute lung injury model. After the wound preparation is completed, the breathing mode is switched to pressure control mode, maintaining stable alveolar pressure through a constant peak airway pressure, promoting the uniform distribution of alveolar surfactant, and reducing the risk of atelectasis caused by mechanical ventilation.

[0048] In some embodiments, the peak airway pressure in the pressure control mode is set to 10-15 cmH2O.

[0049] It should be noted that the peak airway pressure in the pressure control mode is set to This pressure range can simulate the pathological pressure threshold of acute pulmonary edema, and is compatible with both mechanically controlled ventilation and spontaneous breathing modes; the respiratory rate of mechanically controlled ventilation is slightly higher than the animal's spontaneous breathing rate to ensure stable gas exchange.

[0050] In some embodiments, the parameters of the rapid inflation mode are set as follows: the peak airway pressure is 10-15 cmH2O; the duration of a single inflation is 3-7 seconds; and / or the number of breathing cycles of a single inflation is 4-6 times.

[0051] It should be noted that enabling the rapid inflation mode can not only ensure that the lungs are fully inflated to expose the operating field of view, but also avoid additional lung damage or circulatory system disorders caused by continuous high pressure.

[0052] In some embodiments, the electric knife is equipped with a spherical electrode head with a diameter of 1 to 2 mm, and the operating power of the electric knife is 30 to 100 W.

[0053] In some preferred embodiments, when the electrode head of the electrosurgical knife is lightly pressed on the surface of the lung lobe, the contact time between the electrode head and the lung lobe is 3 to 5 seconds, so as to form an eschar wound with a diameter of 3 to 5 mm.

[0054] It should be noted that different combinations of parameters, including a spherical electrode tip with a diameter of 1-2 mm, an operating power of 30-100 W, and a contact time of 3-5 seconds, were obtained through orthogonal experimental optimization. Orthogonal experiments have demonstrated that within this parameter range, a regular eschar wound with a diameter of 3-5 mm can be formed. Furthermore, this parameter combination not only ensures controllable injury depth, but also effectively reduces the risk of secondary damage to surrounding healthy tissues due to heat conduction, ensuring the standardization and reproducibility of model preparation.

[0055] In some embodiments, the method for evaluating air leakage is as follows: use a ventilator to switch the animal's breathing mode to pressure control mode, set the airway peak pressure to 10-15 cmH2O to simulate a dynamic respiratory pressure environment, cover the lung lobe wound with a medical pad, and when the maximum floating amplitude of the pad during the respiratory cycle is greater than or equal to 1 mm, it is determined that air leakage occurs in the lung lobe.

[0056] The embodiment of the present application provides an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. The model is prepared using a preparation method for an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. A regular wound surface that stably produces air leakage exists on the lung lobe.

[0057] In some embodiments, when determining whether the acute lung injury model is successfully prepared, air leakage phenomenon assessment is combined with histopathological assessment to determine;

[0058] Among them, histopathological evaluation includes the following two evaluation methods:

[0059] Evaluation method 1: After the animal model is established, the wound surface is sutured and the animal is artificially reared. Within 3 days, the local inflammatory cell count at the modeling site is semi-quantitatively scored. If the total score is greater than or equal to 9 points, the acute lung injury model is considered to be successfully established. Specific scoring criteria are detailed in Tables 1-2.

[0060] Evaluation method 2: After the animal modeling, the wound surface was sutured and the animals were artificially reared. On the 28th day, the semi-quantitative score of local inflammatory cells, the local pathomorphological score of the modeling tissue, and the local pathomorphological score of local tissue fibrosis were performed on the modeling site of the animals. The total score was calculated at the same time; the total score = semi-quantitative score of inflammatory cells × 20% + local pathomorphological score of modeling tissue × 30% + local pathomorphological score of modeling tissue fibrosis × 50%. When the total score was greater than or equal to 2 points, the acute lung injury model was considered to be successfully established. The specific scoring criteria are detailed in Tables 1 to 3.

[0061] Table 1. Semi-quantitative scoring of inflammatory cells in lung wound tissue (HE staining)

[0062]

[0063] Table 2. Histopathological morphological scores of lung lobe wounds (HE staining)

[0064]

[0065] Table 3. Pathomorphological Scoring of Lung Wound Fibrosis (Masson Staining)

[0066]

[0067] The present application provides an application of an acute lung injury model for evaluating the repair effect of a nano-blocking membrane.

[0068] The present embodiment provides a method for evaluating the repair effect of a nano-occluding membrane, and uses an acute lung injury model to evaluate the repair effect of the nano-occluding membrane.

[0069] In some embodiments, the method for evaluating the repair effect of a nano-blocking membrane comprises the following steps:

[0070] The nano-occluding membrane was used to cover the lung lobe wound in an acute lung injury model, and the occluding and repairing effect of the nano-occluding membrane was evaluated by observing the stability of the nano-occluding membrane.

[0071] It should be noted that the stability of the nano-blocking membrane can be observed through the following multiple dimensions to evaluate the repair effect of the nano-blocking membrane: the physical level needs to consider the adhesion strength and structural integrity of the membrane to the lung lobe wound to ensure that it does not fall off or break; the functional level focuses on monitoring the air leak blocking efficiency and pressure adaptability to determine the sealing ability of the membrane under pressure environment; the biological level focuses on the compatibility of the membrane with lung tissue and its repair effect on the tissue to avoid adverse reactions such as inflammation.

[0072] The applicant further provides the following reference specific embodiments to describe the present invention. It should be noted that these embodiments are merely illustrative and do not limit the present invention in any way.

[0073] Example 1

[0074] This example provides a method for preparing an acute lung injury model suitable for evaluating the repair effect of a nano-blocking membrane. The specific steps are as follows:

[0075] Step S1, preparation of experimental animals and instruments:

[0076] The experimental animals used were New Zealand male rabbits provided by the Guangdong Medical Laboratory Animal Center, with an age range of 90 to 150 days and a weight range of 2.0 to 3.0 kg. The purchased New Zealand rabbits were artificially raised in a single cage in a normal environment for 10 to 14 days, with free access to water and food during the breeding period. The breeding environmental parameters were set as follows: ambient temperature 20 to 25.7°C, daily temperature difference no more than 4°C, relative humidity 30 to 70%, air exchange rate ≥8 times / hour, light-dark ratio of 12 hours:12 hours, working illumination ≥150Lx, animal illumination 100 to 200Lx;

[0077] The equipment included: a POWER-420X high-frequency electrosurgical unit (electrode tip diameter 1.5 mm) manufactured by Changzhou Yanlin Electronic Equipment Co., Ltd.; and a Mindray Medical Veta5 veterinary anesthesia machine, which provides a pressure-controlled respiratory rate of 25 to 40 breaths per minute and a tidal volume of 10 to 15 ml / kg.

[0078] Experimental reagents included: Shutai 50 (3.6 mg / kg, Virbac Co., Ltd., France), xylazine hydrochloride injection (1.4 mg / kg, Dunhua Shengda Animal Pharmaceutical Co., Ltd.), and isoflurane (Jiangsu Hengfengqiang Biotechnology Co., Ltd.);

[0079] S2, animal anesthesia and ventilation pre-settings:

[0080] For S2-1, male New Zealand rabbits weighing 2.8 to 4.0 kg were selected and anesthetized with a mixture of Zolpidem 50 and xylazine hydrochloride injection injected intramuscularly, and 1 to 3% isoflurane inhaled through a ventilator until the animals reached a moderate anesthesia state, as demonstrated by loss of pain reflexes, steady breathing, and muscle relaxation. They were then secured to a sterile laboratory operating table.

[0081] S2-2, connect the ventilator circuit, turn on the pressure control mode, and set the peak airway pressure to , respiratory rate was 40 times / min, and pre-ventilation treatment was performed;

[0082] S3. Preparation of acute lung injury model:

[0083] S3-1: Cut the superficial muscles along the intercostal space of the male rabbit to expose the lung tissue. After selecting the lung injury site, adjust the ventilator to rapid inflation mode, set the peak airway pressure to 10-15 cmH2O, and the single inflation time to no more than 7 seconds (perform 4-6 breathing cycles) to ensure that the male rabbit's lungs are fully expanded.

[0084] S3-2: Adjust the electrosurgical unit power to 30, 50, and 100 W, respectively. Gently press the electrosurgical unit electrode tip vertically onto the lung lobe surface for 3–4 seconds, forming an eschar wound approximately 4 mm in diameter. Peel off the eschar to prevent residual heat from damaging surrounding tissues. A fresh lung lobe wound is now obtained.

[0085] S4. Determination of the success rate of acute lung injury model:

[0086] The ventilator was switched to pressure control mode, and the peak airway pressure was set to 10-15 cmH2O to simulate a dynamic respiratory pressure environment. A sterile medical pad was covered on the wound surface of the lung lobe. It was detected that the maximum floating amplitude of the sterile medical pad during the respiratory cycle was greater than 1 mm, indicating that air leakage occurred in the lung lobe. At this time, the acute lung injury model was successfully prepared.

[0087] To verify the effectiveness of the acute lung injury model, this study systematically detected the air leakage phenomenon in lung tissue. Figures 1-2 This embodiment uses 30W, 50W and 100W power electric knife, strictly follows the preparation method of the present invention to construct the lung lobe wound, and visually observes the air leakage by covering it with a medical pad. Figure 1 As shown in (b)-1(e), during the respiratory cycle of the injured lung lobe, the pad moves regularly with the airflow, which indicates that there is continuous air leakage in the wound. Figure 1 In (a), the intact, healthy lung lobe remains static after being covered with the pad, confirming the integrity of the lung tissue structure and the absence of gas leakage. This demonstrates that the model preparation method presented here can accurately and controllably create lung tissue wounds, successfully simulating the pathological characteristics of acute lung injury and providing a standardized experimental basis for the subsequent scientific evaluation of the repair efficacy of the nano-blocking membrane.

[0088] Example 2

[0089] Based on Example 1, a new method for determining the success of the acute lung injury model is added - histopathological evaluation (evaluation method 2). This method requires simultaneous determination of air leakage. The specific operation method is as follows:

[0090] Step S1, according to the preparation method of Example 1, a fresh lung lobe wound was prepared under the condition of an electrosurgical unit power of 50W. After the acute lung injury model was successfully prepared by evaluating the air leakage phenomenon, the muscle tissue and epidermis of the male rabbit were sutured, and then artificially raised for 28 days;

[0091] Step S2: On the 28th day, the male rabbit was anesthetized and pre-ventilated (this process was consistent with step S2 in Example 1). After the male rabbit reached a moderately anesthetized state, the surface muscle was cut along the intercostal space of the male rabbit to expose the lung tissue. The lung lobe was sliced ​​at the original lung lobe wound site. A total of three sections were taken, and the sections were stained with HE and Masson staining. After fixation with 4% paraformaldehyde (PFA), they were embedded in paraffin. After embedding, observation sections with a thickness of 5 μm were prepared. The degree of inflammatory cell infiltration, tissue necrosis, and peribronchial fibrous tissue repair at the original lung lobe wound site were observed under a 400× high-power microscope (e.g., Figure 3 shown), and combined Figure 3 The histopathological features shown were comprehensively judged according to the scoring criteria in Tables 1 to 3. The specific total score results are shown in Table 4.

[0092] Table 4. Histopathological evaluation results of lung lobe wounds

[0093]

[0094] The slice data in Table 4 show that the total scores of all three slices were higher than 2. This demonstrates that the modeling system and method provided by the present invention did not cause significant organic damage to the experimental animals during the establishment of the acute lung injury model. Furthermore, after routine suturing, the experimental animals achieved a 100% survival rate. This reduces experimental costs by over 50% compared to traditional modeling methods, effectively reducing the number of experimental animals used and fully complying with the technical requirements of the 3R principles (replacement, reduction, and optimization) of experimental animals. The modeling system and method of the present invention not only validates the scientific and reliable nature of the acute lung injury model but also provides an economical and ethically compliant experimental animal model solution.

[0095] The above are only preferred embodiments of the present application and are not intended to limit the present invention. Although the present application has been described in detail with reference to examples, those skilled in the art can still modify the technical solutions described in the aforementioned examples or make equivalent replacements for some of the technical features therein. However, any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present application should be included in the scope of protection of the present application.

Claims

1. An acute lung injury model suitable for evaluating the repair effect of nano-blocking membranes, characterized in that: The acute lung injury model was prepared using the following steps: Step S1, performing combined intramuscular anesthesia and respiratory anesthesia on the animal, with respiratory anesthesia using a pressure-controlled mode; Step S2: Open the animal's surface muscles to expose the lung lobes, and adjust the pressure control mode to a rapid inflation mode to allow the lung lobes to fully expand during inflation; Step S3, lightly pressing the electrode head of the electrosurgical knife on the surface of the lung lobe to form an eschar wound on the surface of the lung lobe, and peeling off the eschar wound to obtain a lung lobe wound; Step S4, adjusting the rapid inflation mode to the pressure control mode, and obtaining an acute lung injury model when air leakage occurs on the lung lobe wound surface; The animal is a rabbit; The peak airway pressure in the pressure control mode is set to 10-15 cmH2O; The parameters of the rapid inflation mode are set as follows: the peak airway pressure is 10-15 cmH2O; the duration of a single inflation is 3-7 seconds; and / or the number of breathing cycles of a single inflation is 4-6 times; The electrosurgical knife is equipped with a spherical electrode head with a diameter of 1 to 2 mm and an operating power of 30 to 100 W. When the electrode head of the electric knife is lightly pressed on the surface of the lung lobe, the contact time between the electrode head and the lung lobe is 3 to 5 seconds, so as to form an eschar wound with a diameter of 3 to 5 mm.

2. The acute lung injury model suitable for evaluating the repair effect of nano-blocking membrane according to claim 1, characterized in that: An acute lung injury model system suitable for evaluating the repair effect of nano-blocking membranes was prepared; The system for the acute lung injury model suitable for evaluating the repair effect of the nano-blocking membrane includes an anesthesia unit, an inflation unit, an electrocoagulation burning unit, and an air leakage testing unit; The anesthesia unit is used to perform combined anesthesia treatment on animals through intramuscular anesthesia and respiratory anesthesia; The inflation unit is used to perform cyclic inflation on the lung lobes of an animal in an anesthetized state so as to cause the lung lobes to produce cyclic undulating motion; The electrocoagulation burning unit is used to form a wound surface on the cyclically undulating surface of the lung lobe; The air leakage testing unit is used to detect whether air leakage occurs in the lung lobe with a wound surface.

3. The acute lung injury model suitable for evaluating the repair effect of nano-blocking membranes according to claim 1, characterized in that: The air leakage evaluation method is as follows: use a ventilator to switch the animal's breathing mode to pressure control mode, set the airway peak pressure to 10-15 cmH2O to simulate a dynamic respiratory pressure environment, cover the lung lobe wound with a medical pad, and determine that air leakage occurs in the lung lobe when the maximum floating amplitude of the pad during the respiratory cycle is greater than or equal to 1 mm.

4. The acute lung injury model suitable for evaluating the repair effect of nano-blocking membranes according to claim 1, characterized in that: To determine whether the acute lung injury model was successfully established, air leakage phenomenon assessment combined with histopathological assessment was used for judgment; The histopathological evaluation includes the following two evaluation methods: Evaluation method 1: After the animal model is established, the wound surface is sutured and the animal is artificially reared. Within 3 days, the local inflammatory cells in the modeling area are semi-quantitatively scored. If the total score is greater than or equal to 9 points, the acute lung injury model is considered to be successfully established; Evaluation method 2: After the animal modeling, the wound surface is sutured and then artificially raised. On the 28th day, the local inflammatory cell semi-quantitative score, local tissue pathomorphological score and local tissue fibrosis pathomorphological score of the modeling site are scored, and the total score is calculated at the same time; the total score = inflammatory cell semi-quantitative score × 20% + local tissue pathomorphological score × 30% + local tissue fibrosis pathomorphological score × 50%. When the total score is greater than or equal to 2 points, the acute lung injury model is considered to be successfully prepared.

5. The acute lung injury model suitable for evaluating the repair effect of nano-blocking membrane according to claim 1, characterized in that: There are regular wounds on the lung lobes that produce stable air leakage.

6. Use of the acute lung injury model according to any one of claims 1 to 5, characterized in that: Used to evaluate the repair effect of nano-sealing membrane.

7. A method for evaluating the repair effect of a nano-blocking membrane, characterized in that: The acute lung injury model described in any one of claims 1 to 5 is used to evaluate the repair effect of the nano-occluding membrane.

8. The method for evaluating the repair effect of nano-blocking membrane according to claim 7, characterized in that: The following steps are involved: The nano-occluding membrane was used to cover the lung lobe wound in an acute lung injury model, and the occluding and repairing effect of the nano-occluding membrane was evaluated by observing the stability of the nano-occluding membrane.

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