Efficient prevention and treatment agent for meadow moth and synergistic application thereof

Abstract

The invention discloses an efficient prevention and control agent for meadow moth and synergistic application of the efficient prevention and control agent, and relates to the field of prevention and control of meadow moth. A leaf immersion method is adopted to measure toxicity and synergistic effect of insecticide and a mixture of the insecticide and an auxiliary agent on meadow moth moth; the toxicity LC50 of 4.5% beta-cypermethrin EC to the meadow moth moth is 1.9539 mg / L, and the beta-cypermethrin EC can be used for preventing and treating the meadow moth moth; when 4.5% of beta-cypermethrin EC is mixed with Gerberi or Jino, LC50 is 1.2006 mg / L and 1.3193 mg / L respectively, co-toxicity coefficients are 162.74 and 148.10 respectively, and the beta-cypermethrin EC and Gerberi or Jino can play a synergistic role. When 4.5% beta-cypermethrin EC is mixed with Jingol or Jinnol, the mixture can be used for preventing and treating meadow moth damage in meadow, crops, vegetables, fruit trees, flowers and Chinese herbal medicines.

Description

Technical Field

[0001] The invention relates to the field of preventing and controlling grass borers, in particular to a highly effective grass borer preventing and controlling agent and its synergistic effect. Background Art

[0002] The meadow moth (Loxostegesticticalis Linnaeus), also known as the yellow-green striped borer and beet web borer, is distributed between 36° and 55°N and has repeatedly caused periodic outbreaks in numerous countries across Asia, Europe, and North America. Its widespread infestation, sudden onset, omnivorous nature, explosive outbreaks, and migratory nature have caused significant economic losses to agriculture and animal husbandry in North, Northwest, and Northeast my country. Currently, chemical control remains the primary control measure during major meadow moth outbreaks, in addition to agricultural, ecological, and biological control. However, the only registered pesticides for meadow moth control in my country are triazophos and chlorpyrifos-triazophos. Therefore, the search for highly effective, low-toxic, and environmentally friendly chemical control agents and pesticide adjuvants that reduce dosage and increase efficiency is urgent.

[0003] Pesticide adjuvants are renowned for their excellent wettability, spreadability, and permeability. They can improve the physical and chemical properties of pesticides, enhancing their effectiveness and reducing the amount of pesticides used. Studies have shown that when mixed with insecticides, pesticide adjuvants can enhance the control of pests such as the diamondback moth (Plutella xylostella), the peach aphid (Myzus persicae), and the fall armyworm (Spodoptera frugiperda), while significantly reducing pesticide usage. Consequently, pesticide adjuvants are widely used in insecticides, fungicides, and herbicides, but their application in the control of fall moths has been largely unreported. Summary of the Invention

[0004] In response to the above-mentioned problems, the present invention proposes a highly effective control agent for grassland borers and its enhanced effect, providing a new high-efficiency, low-toxic and environmentally friendly agent product solution for the efficient control of grassland borers.

[0005] To achieve the above object, the present invention is implemented according to the following technical solutions:

[0006] A highly effective pesticide for preventing and controlling grass borers, the pesticide components comprising highly effective cypermethrin emulsifiable concentrate and Jiexiaoli or Jinnuo.

[0007] In the above technical solution, the pharmaceutical components include 4.5% highly effective chlorpyrifos emulsifiable concentrate and Jiexiaoli or Jinnuo.

[0008] In the above technical solution, the weight ratio of 4.5% highly effective chlorpyrifos emulsifiable concentrate and Jiexiaoli or Jinnuo in the pharmaceutical components is: 100:0.02~0.1.

[0009] In the above technical solution, the weight ratio of 4.5% highly effective chlorpyrifos emulsifiable concentrate and Jiexiaoli or Jinnuo in the pharmaceutical components is 100:0.05.

[0010] The present invention also provides an application of a high-efficiency control agent for preventing and controlling grass borers in preventing and controlling grass borers.

[0011] In the above applications, the agent is used to treat damage to grasslands, crops, vegetables, fruit trees, flowers and Chinese herbal medicine plants.

[0012] In the above application, the method for using the agent is: using any one of the high-efficiency control agents for grassland borers, with a dosage of 30-50 mL / mu, and spraying with a sprayer.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The meadow moth is a migratory, polyphagous, and important agricultural pest that poses a huge threat to agricultural and animal husbandry production in the Three North regions of my country. Therefore, screening for environmentally friendly, highly effective, and low-risk control agents is of great practical significance for ensuring the sustainable development of agriculture and animal husbandry and protecting the ecological environment. The toxicity test of highly effective cypermethrin emulsifiable concentrate against the meadow moth has shown that the toxicity of 4.5% highly effective cypermethrin EC against the meadow moth is LC 50 1.9539mg / L, which can be used to control grass borers; when 4.5% highly effective chlorpyrifos EC is mixed with Jiexiaoli or Jinnuo, LC 50 The concentrations of 4.5% highly effective cypermethrin EC were 1.2006 mg / L and 1.3193 mg / L, respectively, with co-toxicity coefficients of 162.74 and 148.10, indicating a synergistic effect. A mixture of 4.5% highly effective cypermethrin EC with either Jiexiaoli or Jinnuo can be used to control grass borer damage in grasslands, crops, vegetables, fruit trees, flowers, and Chinese herbal medicines. DETAILED DESCRIPTION

[0015] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments.

[0016] Example 1

[0017] 1. Materials and Methods

[0018] 1.1 Test materials

[0019] Test insects: Cocoons of the grass borer Loxostegesticticalis were collected from Wuchuan County, Hohhot City, Inner Mongolia. After emergence, adults were reared in 5% glucose solution at 20-22°C, with a photoperiod of 16h / 8h (L / D) and 70% relative humidity. Larvae were reared on Chenopodium album until they reached the third instar and were used for toxicity testing.

[0020] Test plant: Chenopodium album L., whose leaves were collected from wasteland without pesticide application, were used for feeding the meadow moth and as a carrier in the determination of the physical properties of the solution.

[0021] 1.2 Test agents and adjuvants

[0022] Test agent: 4.5% highly effective cypermethrin emulsifiable concentrate (Shandong Henglida Biotechnology Co., Ltd.).

[0023] The test adjuvants: Jiexiaoli (Maitu High-tech Materials (Nantong) Co., Ltd.), ethoxy-modified polytrisiloxane, silicone adjuvant; Jinnuo (Dongguan Redefeng Biotechnology Co., Ltd.), high-performance spreader; Jijian (Chengdu Jijian Biotechnology Co., Ltd.) composed of honey, rapeseed oil and polyol-type non-ionic surfactants.

[0024] 1.3 Test methods

[0025] 1.3.1 Indoor toxicity determination method for pesticide solution to grass borer

[0026] The leaf dip method was used to determine the indoor toxicity of the pesticide solution against the grass borer. A preliminary test was conducted to determine the concentration range. Toxicity testing was then conducted at five concentration gradients within the mortality range of 10% to 90%. The concentration series of high-efficiency cypermethrin included 20, 10, 5, 2.5, and 1.25 mg / L. A 0.05% adjuvant diluent was used to prepare high-efficiency cypermethrin. The concentration series used for toxicity testing were 4, 2, 1, 0.5, and 0.25 mg / L. The specific method involved soaking fresh quinoa leaves of comparable size in the pesticide for 10 seconds, removing them and allowing them to dry naturally. The leaves were then placed in a Petri dish (9 cm diameter), with ten third-instar larvae per dish. This was repeated three times, with water used as a control. Larvae were observed and counted for 48 hours after incubation at 23°C. The insects were then gently touched with the tip of a brush. Larvae that failed to move normally were considered dead. A valid test was considered effective if the control mortality rate was less than 10%.

[0027] 1.3.2 Determination of physical properties of drug solutions

[0028] Physical properties of the agent include surface tension, contact angle, and drug retention. Surface tension was measured using a JYW-200 automatic surface tension meter, following the ring film method specified in GB / T5549-2010. The instantaneous contact angle of the droplet was measured using a DSA25 standard contact angle meter. Drug retention was determined using the immersion method, following the method of Yuan Huizhu et al. Four treatments were included in this experiment: 0.05% Jiexiaoli plus the recommended dose of the agent (2,000 times), 0.05% Jinnuo plus the recommended dose of the agent (2,000 times), 0.05% Jijian plus the recommended dose of the agent (2,000 times), and distilled water plus the recommended dose of the agent (2,000 times) as a control. Six replicates were used for each physical property.

[0029] 1.3.3 Field control methods of liquid medicine against grassland borer

[0030] The field efficacy study on the control of grass borer was carried out in grassland (natural grassland) and corn field (jointing stage) by selecting 4.5% highly effective chlorpyrifos emulsifiable concentrate (the conventional dosage was set at 40mL / mu, and the dosage was reduced by 25%, i.e. 30mL / mu) and adding the preferred adjuvant Jiexiaoli or Jinnuo after the dosage reduction (both adjuvants were set at 2 different addition ratios, 0.05% and 0.1% respectively). The third instar larvae of grass borer were selected for control. Each treatment was repeated 3 times and arranged in random blocks. The area of each plot was 60m 2 A backpack electric sprayer was used to spray the entire plant in the plot. The water spraying treatment served as the control (CK).

[0031] Before spraying, three points were randomly surveyed in each plot, with a length of 1 m 2 The number of live insects was recorded. On the 7th day after application, the number of live insects was counted again, and the insect population reduction rate and control effect were calculated. SPSS software was used for statistical analysis, and Duncan's new multiple range method was used for significant difference test.

[0032] Pest population reduction rate (%) = [(number of live insects in the treatment area before treatment - number of live insects in the treatment area after treatment) / (number of live insects in the treatment area before treatment)] × 100

[0033] Control effect (%) = [(pest population reduction rate in the treated area - pest population reduction rate in the control area) / (100 - pest population reduction rate in the control area)] × 100

[0034] 1.4 Data Processing

[0035] The slope, standard error, and lethal concentration (LC) of the toxicity equation of the tested agents and the adjuvant-added highly effective cypermethrin to the meadow borer were calculated using EXCEL analysis software. 50 ), 95% confidence interval and co-toxicity coefficient after adding adjuvant.The data of physical properties of the liquid were statistically analyzed using SPSS software, and Duncan's new multiple range method was used for significant difference test.

[0036] Co-toxicity coefficient (CTC) = LC 50 (Single dose) / LC 50 (single dose + adjuvant)×100.

[0037] When CTC>120, it is a synergistic effect; when CTC is 80-120, it is an additive effect; when CTC<80, it is an antagonistic effect.

[0038] 2. Results and Analysis

[0039] 2.1 Indoor toxicity of highly effective cypermethrin to the meadow borer

[0040] The results of the indoor toxicity test of cypermethrin against the grass borer are shown in Table 1. The toxicity LC value of 4.5% cypermethrin EC is 50 The concentration of 4.5% highly effective cypermethrin EC is 1.9539 mg / L. Due to its low price, it is widely used in the emergency prevention and control of grassland borers.

[0041] Table 1 Toxicity of highly effective cypermethrin to meadow borer

[0042]

[0043] 2.2 Indoor toxicity of a mixture of highly effective cypermethrin and three adjuvants to S.

[0044] The mixture of high-efficiency cypermethrin and different adjuvants can play an additive and synergistic role in the indoor toxicity of grass borer, and the co-toxicity coefficient ranges from 106.35 to 162.74. The results are shown in Table 2. 50 The concentrations of chlorpyrifos and cypermethrin in the mixture were 1.200 6mg / L and 1.319 3mg / L, respectively, and the co-toxicity coefficients were 162.74 and 148.10, respectively. Therefore, Jiexiaoli and Jinnuo can be used as mixing adjuvants for the insecticide cypermethrin for controlling grass borers.

[0045] Table 2 Combined toxicity of cypermethrin and pesticide adjuvant mixtures to S.

[0046]

[0047] 2.3 Effects of pesticide adjuvants on the physical properties of cypermethrin solution

[0048] Pesticide adjuvants significantly reduced the surface tension of the cypermethrin solution and its contact angle with the leaves of Chenopodium album, increasing its retention (Table 3). The surface tensions of the cypermethrin solution (unadjuvanted control) and the treatments with Jiexiaoli, Jinnuo, and Jijian were 59.88, 47.40, 49.50, and 54.70 mN / m, respectively. The surface tensions of the solution containing the adjuvants were significantly lower than those of the recommended dose of cypermethrin (unadjuvanted control), and there were significant differences among the three adjuvant-added treatments.

[0049] The contact angles of quinoa leaves with beta-cypermethrin (unadjuvanted control) and treatments with Jiexiaoli, Jinnuo, and Jijian were 86.07°, 0.00°, 59.32°, and 66.59°, respectively. The contact angles of the pesticide adjuvanted solutions were significantly lower than those of the recommended dose of beta-cypermethrin (unadjuvanted control), and there were significant differences among the three adjuvanted treatments. In particular, the Jiexiaoli solution expanded immediately upon contact with the leaves, resulting in a contact angle of 0.00.

[0050] The retention rates of high-efficiency cypermethrin (control without adjuvant) and the treatments with Jiexiaoli, Jinnuo and Jijian on Chenopodium album leaves were 7.41, 13.59 and 11.87 mg / cm, respectively. 2 and 10.25 mg / cm 2 The retained amount of the pesticide solution with added pesticide adjuvants was significantly greater than the retained amount of the recommended dose of cypermethrin (control without adjuvants).

[0051] Table 3 Physical properties and retention of cypermethrin solution after adding pesticide adjuvants

[0052]

[0053] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level tested by Duncan's new multiple range test.

[0054] 2.4 Field efficacy of a mixture of pesticide adjuvants and highly effective cypermethrin against meadow borers

[0055] The study found that, as shown in Table 4, 4.5% highly effective cypermethrin emulsifiable concentrate (40 mL / mu) achieved an 86.4% control efficacy against grass borer after 7 days on grassland, significantly superior to the 74.2% efficacy achieved when the dosage was reduced by 25% (i.e., 30 mL / mu) (P < 0.05). Compared with no adjuvant, adding the preferred adjuvants Jiexiaoli or Jinnuo (including two different addition ratios of 0.05% and 0.1%) after reducing the dosage significantly improved the control efficacy against grass borer, and there was no significant difference in efficacy compared to the conventional 40 mL / mu dosage. Therefore, adding the adjuvant Jiexiaoli or Jinnuo (at 0.05% or 0.1%) can achieve the goal of reducing dosage and increasing efficacy.

[0056] Table 4 Field control effect of high-efficiency cypermethrin mixed with pesticide adjuvants on meadow borer (grassland)

[0057]

[0058]

[0059] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level tested by Duncan's new multiple range test.

[0060] As shown in Table 5, 4.5% highly effective cypermethrin emulsifiable concentrate (EC) at the standard dosage of 40 mL / mu achieved a 90.1% control efficacy against corn borers after 7 days, significantly superior to the 68.3% efficacy achieved at a 25% reduction in dosage (30 mL / mu) (P < 0.05). Compared with no adjuvant, adding the preferred adjuvants Jiexiaoli or Jinnuo (at two different addition rates, 0.05% and 0.1%) significantly improved control efficacy against corn borers, with no significant difference in efficacy compared to the standard dosage of 40 mL / mu. Therefore, adding either Jiexiaoli or Jinnuo (at either 0.05% or 0.1%) can achieve the goal of increasing efficacy with reduced dosage.

[0061] Table 5 Field control effect of high-efficiency cypermethrin mixed with pesticide adjuvants on meadow borer (corn field)

[0062]

[0063] Note: Different letters after the data in the same column indicate significant differences at the 0.05 level tested by Duncan's new multiple range test.

[0064] 3 Conclusion and Discussion

[0065] The meadow moth (Lepidoptera: sphaeroides) is a migratory, polyphagous, and important agricultural pest, posing a significant threat to agricultural and animal husbandry production in my country's "Three Norths" region. Therefore, identifying environmentally friendly, highly effective, and low-risk control agents is of great practical significance for ensuring the sustainable development of agriculture and animal husbandry and protecting the ecological environment. High-efficiency cypermethrin has an efficacy of 86% to 90%. Based on the results of this experiment, using Jiexiaoli or Jinnuo as a blending adjuvant can reduce dosage by 25% and increase efficacy. This enhanced efficacy is primarily due to improved physical and chemical properties of the agent, such as reducing the surface tension of the solution on the host leaves, increasing the amount of solution retained on the leaves, improving control effectiveness, reducing the dosage, and achieving environmental protection. This study suggests that its widespread use in production is feasible.

[0066] The above description is merely a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by a person skilled in the art within the technical scope disclosed in the present invention should be included in the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be based on the scope of protection of the claims.

Claims

1. A highly effective control agent for grassland borer, characterized in that: The pharmaceutical components include highly effective cypermethrin emulsifiable concentrate and Jiexiaoli or Jinnuo.

2. A highly effective control agent for grass borer according to claim 1, characterized in that: The pharmaceutical composition comprises 4.5% highly effective cypermethrin emulsifiable concentrate and Jiexiaoli or Jinnuo.

3. A highly effective control agent for grass borer according to claim 2, characterized in that: The weight ratio of 4.5% highly effective chlorpyrifos emulsifiable concentrate to Jiexiaoli or Jinnuo in the pharmaceutical components is 100:0.02-0.

1.

4. A highly effective control agent for grass borer according to claim 3, characterized in that: The weight ratio of 4.5% highly effective chlorpyrifos emulsifiable concentrate to Jiexiaoli or Jinnuo in the pharmaceutical components is 100:0.

05.

5. Use of the highly effective control agent for grassland borer according to any one of claims 1 to 4 in controlling grassland borer.

6. The use according to claim 5, wherein the agent is used to treat damage to grasslands, crops, vegetables, fruit trees, flowers and Chinese herbal medicines.

7. The use according to claim 5, wherein the method for using the agent is: using the high-efficiency control agent for grassland borer according to any one of claims 1 to 4 at a dosage of 30-50 mL / mu by spraying with a sprayer.

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