A cream containing moringa oleifera seed oil ceramide, preparation method and application thereof

Abstract

The present application belongs to the technical field of cosmetics, and particularly relates to a cream containing Mauritia flexuosa fruit oil ceramide, a preparation method and application. Specifically, the cream is prepared by taking Mauritia flexuosa fruit oil ceramide, batyl alcohol and ruscogenin as main components. The cream containing the combination of Mauritia flexuosa fruit oil ceramide, batyl alcohol and ruscogenin can significantly promote the generation of barrier-related protein FLG and the generation of laminin, has the effects of repairing skin barrier and anti-aging of the true epidermal junction layer, can significantly improve the problem of large high-low temperature emulsification particle size of the cream containing high content of ceramide, is mild and non-irritating, and thus has good practical application value.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a cream containing ceramide from palmetto fruit oil, its preparation method, and its application. Background Technology

[0002] The information disclosed in this background section is intended only to enhance understanding of the overall background of the invention and is not necessarily to be construed as an admission or in any way implying that such information constitutes prior art known to those skilled in the art.

[0003] Skin aging is a complex biological process, influenced by both internal factors such as natural aging and external factors such as environment and lifestyle. It manifests as a decline in skin barrier function, breakage of elastic fibers, a decrease in laminin content, and accelerated epidermal moisture loss, thus exacerbating signs of aging such as wrinkles, dryness, and sensitivity. Currently, many anti-aging products contain antioxidants such as vitamin C, resveratrol, peptides, and retinol. However, these products have limitations, including a single pathway of action and difficulty in simultaneously addressing the synergistic deterioration of the skin barrier and aging. Furthermore, some ingredients, such as vitamin C and retinol, can irritate the skin, easily causing adverse reactions such as erythema and peeling, making them unsuitable for sensitive skin.

[0004] In recent years, ceramides have become a research hotspot in the anti-aging field due to their dual repair properties of strengthening the skin barrier and restoring skin moisture. Among them, ceramides derived from natural plants have a similar lipid structure to the human stratum corneum and have the advantage of being environmentally friendly and sustainable. However, the inventors have found that existing technologies still have the following bottlenecks: the efficacy of plant ceramides when used alone is limited, their bioavailability is low, and they are difficult to cover the multi-dimensional repair mechanisms of aging; moreover, in traditional cream systems, high ceramide content can easily cause the cream emulsification to increase in particle size under high and low temperature conditions, resulting in problems such as discontinuous application and rough texture. Summary of the Invention

[0005] To address the shortcomings of existing technologies, the present invention aims to provide a cream containing ceramides from *Palmeria rubra* fruit oil, its preparation method, and its applications. Specifically, the present invention provides a cream prepared with ceramides from *Palmeria rubra* fruit oil, squalene, and rusco saponin as the main ingredients. Experimental verification has shown that the cream containing the combination of ceramides from *Palmeria rubra* fruit oil, squalene, and rusco saponin can significantly promote the generation of barrier-related proteins (FLG) and laminin, thus exhibiting skin barrier repair and anti-aging effects on the dermal-epidermal junction. Simultaneously, it can significantly improve the problem of increased particle size due to high-temperature emulsification in creams containing high ceramide content, and is gentle and non-irritating. Based on the above research results, the present invention is thus completed.

[0006] To achieve the above-mentioned technical objectives, the present invention provides the following technical solution:

[0007] A first aspect of the present invention provides a cream containing ceramides from palmetto fruit oil, said cream comprising at least the following components in parts by weight:

[0008] The fruit oil contains 0.1-5 parts ceramide, 0.05-0.5 parts squalene, and 0.01-0.1 parts rusco saponin.

[0009] Furthermore, the components include: 1.5-3 parts of ceramide from mulberry fruit oil, 0.2-0.4 parts of squalene, and 0.05-0.08 parts of rusco saponin.

[0010] Furthermore, the cream also contains cosmetic excipients commonly used in cream formulations, such as emulsifiers, oils, polyols, thickeners, preservatives, chelating agents, and solvents.

[0011] The ceramide in the morrhagic palm fruit oil is obtained by reacting morrhagic palm fruit oil fatty acids with sphingosine compounds.

[0012] A second aspect of the present invention provides a method for preparing the above-mentioned cream containing ceramide from palmetto fruit oil, the method comprising:

[0013] S1. Preparation of the aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80℃ and stir to dissolve;

[0014] S2. Preparation of the oil phase: Heat the oil phase emulsifier and oil to 70-75℃ to melt. After complete melting, add ceramide from morrhagic fruit oil and squalene before phase combination, and stir until the mixture is uniformly melted.

[0015] S3. Homogenize the aqueous and oil phases; after cooling, add rusco saponin; furthermore, rusco saponin needs to be pre-dissolved by heating butanediol to 85°C and stirring.

[0016] S4: Cool to 65-70℃, add preservative, stir to dissolve, then stir and cool to about 38℃ to obtain the product.

[0017] A third aspect of the present invention provides the use of the above-mentioned cream containing ceramide from palmetto fruit oil in the preparation of cosmetics.

[0018] A fourth aspect of the present invention provides a cosmetic product comprising at least the above-mentioned cream containing ceramide from palmetto fruit oil. The cosmetic product is a skincare product.

[0019] The beneficial technical effects of one or more of the above technical solutions are as follows:

[0020] (1) In the stability study of cream systems containing high content of plant ceramides, there is a problem that the emulsion particle size increases under high temperature and cold and hot cycling conditions. The combination of ceramide from morrhagic palm fruit oil, squalene, and rusco saponin can synergistically improve the problem of increased emulsion particle size in creams containing high content of plant ceramides, and greatly improve the stability of the cream.

[0021] (2) In vitro tests have verified that the cream containing the combination of ceramide, squalene, and rusco saponin can achieve the effect of repair and anti-aging by synergistically promoting the production of barrier-related proteins FLG and laminin. Moreover, the synergistic effect of the cream containing the combination of ceramide, squalene, and rusco saponin is better than that of the cream containing the combination of ceramide, squalene, and rusco saponin.

[0022] (3) Human trials have verified that the cream prepared by this invention can significantly reduce the TEWL value and improve skin elasticity R2 and R7, and has good human body repair and anti-aging effects.

[0023] (4) The preparation method of the cream containing the combination of palm fruit oil ceramide, squalene and rusco saponin obtained by the above technical solution is simple and easy to implement, saves costs, the raw materials are easy to obtain, and it is suitable for mass production. Through human efficacy test, the anti-aging effect and repair effect are significant, so it has good practical application value. Attached Figure Description

[0024] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0025] Figure 1 Example 3 of the present invention: Microstructure image after three months of observation at 45°C;

[0026] Figure 2 Comparative Example 3 of this invention is a microstructure image after three months of observation at 45°C. Detailed Implementation

[0027] It should be noted that the following detailed description is illustrative and intended to provide further explanation of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0028] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0029] In a typical embodiment of the present invention, a cream containing ceramide from palmetto fruit oil is provided, the cream comprising at least the following components in parts by weight:

[0030] The fruit oil contains 0.1-5 parts ceramide, 0.05-0.5 parts squalene, and 0.01-0.1 parts rusco saponin.

[0031] Furthermore, the components include: 1.5-3 parts of ceramide from mulberry fruit oil, 0.2-0.4 parts of squalene, and 0.05-0.08 parts of rusco saponin.

[0032] Furthermore, the cream also contains cosmetic excipients commonly used in cream formulations, such as emulsifiers (further examples include hydrogenated lecithin, arachidonic acid / behenol / arachidonic acid glucoside), oils (further examples include dioctyl carbonate, isononyl isononanoate, polydimethylsiloxane, caprylic / capric triglyceride, behenol, cetearyl alcohol), polyols (further examples include glycerin, dipropylene glycol, butylene glycol), thickeners (further examples include sodium polyacrylate, ammonium acryloyl dimethyl taurate / VP copolymer), preservatives (further examples include 1,2-hexanediol, p-hydroxyacetophenone), chelating agents (further examples include disodium EDTA), and solvents (such as water), etc., without specific limitations.

[0033] The ceramide in the *Morris palmatum* fruit oil is obtained by reacting the fatty acids in *Morris palmatum* fruit oil with sphingosine compounds, wherein the sphingosine compounds are selected from sphingosine, phytosphingosine, or dihydrosphingosine. Furthermore, the fatty acids in the *Morris palmatum* fruit oil can be obtained from *Morris palmatum* fruit oil through a saponification reaction.

[0034] Specifically, the cream contains at least the following components in parts by weight:

[0035] The ingredients are: 0.1-5 parts ceramide from mulberry fruit oil, 0.05-0.5 parts squalene, 0.01-0.1 parts rusecosanoside, 0.3-5.0 parts emulsifier, 5.0-20.0 parts oil, 2.0-10.0 parts polyol, 0.1-1.0 parts thickener, 0.3-1.5 parts preservative, 0.05-0.20 parts chelating agent, and 90-100 parts water.

[0036] Preferably, the cream contains at least the following components in parts by weight:

[0037] The ingredients are: 1.5-3 parts ceramide from mulberry fruit oil, 0.2-0.4 parts squalene, 0.05-0.08 parts rusecoside, 2.0-5.0 parts emulsifier, 6.0-12.0 parts oil, 3.0-8.0 parts polyol, 0.5-1.0 parts thickener, 0.3-0.8 parts preservative, 0.05-0.15 parts chelating agent, and 93-98 parts water.

[0038] In another specific embodiment of the present invention, a method for preparing the above-mentioned cream containing ceramide from palmetto fruit oil is provided, the method comprising:

[0039] S1. Preparation of the aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80℃ and stir to dissolve;

[0040] S2. Preparation of the oil phase: Heat the oil phase emulsifier and oil to 70-75℃ to melt. After complete melting, add ceramide from morrhagic fruit oil and squalene before phase combination, and stir until the mixture is uniformly melted.

[0041] S3. Homogenize the aqueous and oil phases; add rusco saponin when the temperature drops to about 70°C; furthermore, the rusco saponin needs to be pre-dissolved by heating butanediol to 85°C and stirring before being added.

[0042] S4: Cool to 65-70℃, add preservative, stir to dissolve, then stir and cool to about 38℃ to obtain the product.

[0043] In another specific embodiment of the present invention, the application of the above-mentioned cream containing ceramide from palmetto fruit oil is provided in the preparation of cosmetics.

[0044] In another specific embodiment of the present invention, a cosmetic is provided, which at least comprises the above-mentioned cream containing ceramide from palmetto fruit oil. The cosmetic is a skin care product. Experiments have shown that the cosmetic prepared by the present invention can significantly improve the problem of increased emulsification particle size in creams. In terms of efficacy, it can significantly promote the production of barrier-related protein FLG and the production of dermal-epidermal junction-related protein laminarin. In terms of human efficacy, it can significantly reduce TEWL values ​​and improve skin elasticity R2 and R7, exhibiting significant repair and anti-aging effects, while being gentle and non-irritating.

[0045] The present invention will be further described below with reference to embodiments. The present invention is further illustrated by means of embodiments, but this does not limit the present invention to the scope of the embodiments described. Based on the embodiments of the present invention, any changes to the present invention by those skilled in the art without inventive step are within the protection scope of the present invention. Furthermore, in the embodiments of the present invention, unless otherwise specified, all raw materials used in the preparation are commercially available products well known to those skilled in the art.

[0046] In the embodiments and comparative examples of this invention, the ceramide from the fruit oil of *Pterocarya stenoptera* was prepared using the following method:

[0047] S1: Mix 50g of Mauritius fruit oil with 100ml of ethanol and stir to dissolve. Slowly add 80ml of potassium hydroxide solution (20wt%) at room temperature. After the addition is complete, raise the temperature to 65℃ and monitor the reaction by TLC until saponification is complete. After complete saponification, add 3mol / L dilute hydrochloric acid to adjust the pH to 3. Add 150ml of ethyl acetate to extract the aqueous phase. Extract three times. Combine the aqueous extracts and wash once with 50ml of saturated brine. Dry the organic phase with anhydrous Na2SO4 (1g / 10ml organic phase), filter, and concentrate under vacuum to obtain 44g of Mauritius fruit oil fatty acids.

[0048] S2: 50 mmol of *Malus baccata* fruit oil fatty acids (based on the main fatty acid component), 78 mmol of HOBt (hydroxybenzotriazole), 65 mmol of EDCI (1-ethyl-(3-dimethylaminopropyl)carbodiimide hydrochloride), and 65 mmol of DIPEA (N,N-diisopropylethylamine) were added to a 250 mL round-bottom flask, followed by 100 mL of dichloromethane and 25 mL of DMF (dimethylformamide). The mixture was stirred at room temperature for 1.5 hours. Then, 55 mmol of phytosphingosine was added to the reaction system, and the mixture was stirred at room temperature until the reaction was complete as detected by TLC. After the reaction was complete, water was added to quench the reaction. The organic layer was separated, dried, filtered, and concentrated under vacuum. After washing with solvent, *Malus baccata* fruit oil ceramide was obtained. The fraction containing ceramide was purified by gradient column chromatography, and concentrated to dryness at 45 °C to obtain 15.4 g of white to pale yellow solid, i.e., *Malus baccata* fruit oil ceramide.

[0049] S3: HPLC analysis revealed the following: oleic acid-phytosphingosine ceramide 67%; linoleic acid-phytosphingosine ceramide 15.5%; palmitic acid-phytosphingosine ceramide 10%; stearic acid-phytosphingosine ceramide 2.5%; linolenic acid-phytosphingosine ceramide 0.3%.

[0050] Examples 1-4 and Comparative Examples 1-8:

[0051] The specific plan is shown in Table 1 below, calculated in parts by weight.

[0052] Table 1. List of components for specific embodiments and comparative examples.

[0053]

[0054]

[0055] Note: The sea buckthorn oil ceramide in the comparative example was purchased from Shenzhen Dickman Biotechnology Co., Ltd.

[0056] Preparation method:

[0057] S1: Preparation of the aqueous phase: Add the raw materials from phase A separately, heat to 80℃ and stir to dissolve;

[0058] S2: Preparation of oil phase: Add B phase raw materials except for morrhagic palm fruit oil ceramide (sea buckthorn oil ceramide), squalene, and sea buckthorn oil ceramide, heat to 75℃ to melt, and wait until completely dissolved. Add B phase before merging morrhagic palm fruit oil ceramide (sea buckthorn oil ceramide), squalene, and sea buckthorn oil ceramide, and stir until uniformly melted.

[0059] S3: Combine phase A and phase B, start homogenization (3000 rpm), homogenize for 12 min, and start cooling after homogenization is complete; S4: When the temperature drops to 70℃, add phase C. Among them, rusco saponin needs to be pre-dissolved by heating butanediol to 85℃ and stirring before adding it directly to the combined phase liquid at 70℃. Stir evenly and continue cooling.

[0060] S5: When the temperature drops to 65℃, add phase D, stir to dissolve, and continue to cool down after dissolution is complete.

[0061] S6: Stop stirring when the temperature drops to 38°C.

[0062] Test Example 1: Stability Test

[0063] The samples from Examples 1-4 and Comparative Examples 1-8 were subjected to stability observation at -18℃, 4℃, and 45℃, respectively. Microscopic observation was performed weekly at -18℃, 4℃, and 45℃ for a total of 12 weeks to observe whether the emulsified particle size showed a significant increasing trend. The scoring criteria were as follows: no particle size increase within the 12-week observation period resulted in a score of 13; particle size increase within the 10-12 week observation period resulted in a score of 10-12; particle size increase within the 7-9 week observation period resulted in a score of 7-9; particle size increase within the 4-6 week observation period resulted in a score of 4-6; and particle size increase within ≤3 weeks observation period resulted in a score of 1-3. The results are shown in Table 2.

[0064] Table 2 Stability test results

[0065] -18℃ 4℃ 45℃ Average score Example 1 13 13 13 13.0 Example 2 13 13 13 13.0 Example 3 13 13 13 13.0 Example 4 13 13 13 13.0 Comparative Example 1 8 13 3 8.0 Comparative Example 2 10 13 10 11.0 Comparative Example 3 10 13 6 9.7 Comparative Example 4 9 13 5 9.0 Comparative Example 5 8 13 4 8.3 Comparative Example 6 10 13 8 10.3 Comparative Example 7 10 13 8 10.7 Comparative Example 8 10 13 7 10.0

[0066] From Table 2, Figure 1 , Figure 2 It can be seen that Examples 1-4 have good stability, while Comparative Examples 1-8 all show a trend of increasing emulsion particle size to varying degrees. This indicates that the ceramide, squalene, and rusco saponin in the morrhagic fruit oil can synergistically improve the problem of increased emulsion particle size in creams at high and low temperatures, avoiding unstable factors such as discontinuous application and rough texture caused by increased particle size.

[0067] Experimental Example 2: Stimulus Evaluation Experiment

[0068] The experiment recruited 30 participants (2 males and 28 females) aged 18 to 60.

[0069] Human skin occlusive patch test method: The test samples of Examples 1-4 were diluted with distilled water to a 1% solution as the test substance, and distilled water was used as the control group. A qualified patch applicator was selected, and 0.020–0.025 mL of the test substance was placed in the applicator chamber. The patch applicator containing the test substance was applied to the flexor side of the subject's forearm for 24 hours. After 24 hours, the patch applicator was removed, and skin reactions were observed at 0.5 hours, 24 hours, and 48 hours after removal. The results were recorded according to the skin reaction grading standards in the "Cosmetic Safety Technical Specifications 2015" (Table 4). Detailed results are shown in Table 3. According to the interpretation of results in the "Cosmetic Hygiene Standards (2007 Edition)," the criteria for interpreting the results of the occlusive patch test are: if more than 5 out of 30 subjects experience a grade 1 adverse skin reaction, or more than 2 out of 30 subjects experience a grade 2 adverse skin reaction, or if any one subject experiences a grade 3 or higher adverse skin reaction, the test substance is considered to have an adverse skin reaction in humans.

[0070] Table 3. Grading Standards for Skin Reaction

[0071]

[0072] The test results are shown in Table 4. No adverse reactions were observed in Examples 1-4, indicating that the cream containing the combination of ceramide from palm fruit oil, squalene, and rusco saponin is mild and non-irritating.

[0073] Table 4 Results of the Stimulus Evaluation Experiment

[0074]

[0075]

[0076] Experiment 3: Barrier-related protein FLG assay

[0077] use Experiments were conducted using a 3D skin model. Preparation of 0.1% SLS working solution: 1 mL of 0.2% SLS solution was added to 1 mL of PBS to prepare 0.1% SLS working solution; Preparation of positive control (WY14643) working solution: 10 μL of 30 mM WY14643 stock solution was dissolved in 6 mL of culture medium (EpiGrowth medium) to prepare 50 μM WY14643.

[0078] According to the test protocol in Table 5, the model was transferred to a 6-well plate (with 0.9 mL of EpiGrowth medium added beforehand), and the test group number was labeled on the 6-well plate. 25 μL of 0.1% SLS solution was added to the surface of the negative control group (NC), positive control group (PC), Examples 1-4, and Comparative Examples 1-8, and incubated for 30 min. After incubation, the positive control group was treated with the corresponding concentration of working solution, and the test samples (samples from Examples 1-4 and Comparative Examples 1-8) were collected.

[0079] 12.5 μL was added to the model surface, spread evenly, and then incubated in a CO2 incubator (37℃, 5% CO2) for 24 h. After incubation, the surface of the model was cleaned with sterile PBS solution to remove any remaining test material, and the residual liquid inside and outside the model was wiped away with sterile cotton swabs.

[0080] Immunofluorescence assay: The model used for detection was circumcised and fixed with 4% paraformaldehyde. After 24 hours of fixation, the FLG content was detected by immunofluorescence. The images were then photographed and analyzed under a microscope.

[0081] GraphPad Prism was used for plotting, and the results are expressed as Mean ± SD. t-tests were used for comparisons between groups. All statistical analyses were two-tailed. A p-value of 0.01 < 0.05 was considered statistically significant, and a p-value of 0.01 < 0.01 was considered highly statistically significant.

[0082] Table 5: Test Plan

[0083]

[0084]

[0085] Table 6: Summary Table of FLG Content Detection Results

[0086]

[0087] (Note: ## indicates p < 0.01 compared to the blank control; ** indicates p < 0.01 compared to the negative control; * indicates p < 0.01 compared to the negative control.) <p<0.05。)

[0088] As shown in Table 6, Examples 1-4, Comparative Examples 1, and 3-7 significantly promoted FLG protein expression. Comparative Example 2, containing only squalene, and Comparative Example 8, without the composition, did not significantly promote FLG gene expression. The enhancement rates of Examples 1-4 were significantly higher than those of Comparative Examples 1 and 3-7, and the enhancement rate of Example 3 was higher than the sum of the enhancement rates of Comparative Examples 1-3, indicating that the combination of *Hippophae rhamnoides* fruit oil ceramide, squalene, and rusco saponin can synergistically promote FLG protein expression. The enhancement rate of Example 3 was significantly higher than that of Comparative Example 7, indicating that the synergistic effect of the combination of *Hippophae rhamnoides* fruit oil ceramide, squalene, and rusco saponin is superior to that of the combination of sea buckthorn oil ceramide, squalene, and rusco saponin.

[0089] Experiment 4: Laminin (LN) Assay

[0090] Cells in the logarithmic growth phase were digested and centrifuged, and a cell suspension of appropriate density was prepared with complete culture medium. The suspension was then seeded into 6-well cell culture plates according to the experimental design (Table 7) and incubated at 37°C in a 5% CO2 incubator for 24 ± 2 h. In each well of the seeded 6-well plate, complete culture medium was added to the blank control group, complete culture medium containing 40 μM resveratrol was added to the positive control group, and complete culture medium containing a specific concentration of the sample was added to the sample group, 2 mL per well. The 6-well plates were then incubated in a CO2 incubator for 24 ± 2 h. After incubation, the original culture medium in the cell culture plates was discarded, and the plates were washed three times with Duchenne phosphate-buffered saline (DPBS). The cells were then analyzed by Western blotting.

[0091] Quantitative data are expressed as mean ± standard deviation (X ± s). Data are analyzed by one-way ANOVA, and statistical differences are judged by p-value. P < 0.05 indicates statistical significance.

[0092] Laminin upregulation rate (%) = (Laminin expression level in sample group - Laminin expression level in blank control group) / Laminin expression level in blank control group * 100

[0093] Table 7 Test Plan

[0094]

[0095] Table 8 Summary of Laminin Expression Levels

[0096]

[0097] (Note: ** indicates p < 0.01 compared to the blank control; * indicates p < 0.01 compared to the blank control.) <p<0.05。)

[0098] As shown in Table 8, compared with group BC, the expression level of laminin in group PC was significantly increased, indicating that the positive control in this test was effective. Compared with group BC, Examples 1-4 and Comparative Examples 1, 2, and 4-7 significantly promoted laminin expression, while Comparative Example 3, which contained only ruscosaponin, and Comparative Example 8, which did not contain the combination of ceramide, squalene, and ruscosaponin from *Hippophae rhamnoides* fruit oil, did not significantly promote laminin expression. The enhancement rate of Examples 1-4 was significantly higher than that of Comparative Examples 1, 2, and 4-7, and the enhancement rate of Example 3 was higher than the sum of the enhancement rates of Comparative Examples 1-3, indicating that the combination of ceramide, squalene, and ruscosaponin from *Hippophae rhamnoides* fruit oil synergistically promotes laminin expression. The enhancement rate of Example 3 was significantly higher than that of Comparative Example 7, indicating that the synergistic effect of the combination of ceramide, squalene, and ruscosaponin from *Hippophae rhamnoides* fruit oil was superior to that of the combination of ceramide, squalene, and ruscosaponin from *Hippophae rhamnoides* oil.

[0099] Experimental Example 5: Evaluation of Human Body Repair and Anti-aging Efficacy

[0100] Example 3 was tested for its skin repair and anti-aging effects on human skin. A total of 30 participants completed the test, including 8 males and 22 females, aged 30 to 45 years, with an average age of 32.15 ± 5.50 years, meeting the criteria for voluntary inclusion and exclusion of participants.

[0101] How to use: After using toner, take an appropriate amount of sample and apply it evenly to the face. Pat gently until absorbed, then proceed with your normal skincare routine. Use once in the morning and once in the evening, keeping other skincare habits unchanged, for 28 consecutive days.

[0102] Testing time: before using the sample (D0), 7 days after using the sample (D7), and 28 days after using the sample (D28).

[0103] Testing instruments: Vapormeter SWL5001 transdermal water loss meter and Cutometer MPA580 (CK GmbH, Germany) skin elasticity meter.

[0104] Test requirements: Perform the test 3 times and take the average value.

[0105] Parameter explanation: The lower the TEWL value, the less transepidermal water loss; the higher the R2 and R7 values, the higher the skin firmness and the better the anti-aging effect.

[0106] The results are shown in Table 9-11.

[0107] Table 9. Descriptive statistics of TEWL values ​​(n=30)

[0108]

[0109] Statistical analysis results: "**": The difference was statistically significant (0.001 ≤ P < 0.01); "***": The difference was statistically significant (P < 0.001).

[0110] Table 10. Descriptive statistics of skin elasticity R² values ​​(n=30)

[0111]

[0112] Statistical analysis results: "**": The difference was statistically significant (0.001 ≤ P < 0.01); "***": The difference was statistically significant (P < 0.001).

[0113] Table 11 Descriptive statistics of skin elasticity R7 values ​​(n=30)

[0114]

[0115] Statistical analysis results: "***": The difference was statistically significant (P<0.001)

[0116] As shown in Tables 9-11, the skin TEWL value decreased significantly after 7 and 28 days of use, the skin elasticity R2 increased significantly after 7 and 28 days of use, and the skin elasticity R7 increased significantly after 7 and 28 days of use. The results show that the cream containing the combination of ceramide from palm fruit oil, squalene, and rusco saponin has significant effects on human body repair and anti-aging.

[0117] It should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit it. Although the present invention has been described in detail with reference to the given examples, those skilled in the art can modify or make equivalent substitutions to the technical solutions of the present invention as needed, without departing from the spirit and scope of the technical solutions of the present invention.

Claims

1. A cream containing ceramides from murine palm fruit oil, characterized in that, The cream contains at least the following components in parts by weight: The fruit oil of *Pterocarya stenoptera* contains 0.1-5 parts ceramide, 0.05-0.5 parts squalene, and 0.01-0.1 parts rusco saponin. The ceramide from the fruit oil of *Morris palmatum* is obtained by reacting fatty acids from *Morris palmatum* fruit oil with sphingosine compounds, wherein the sphingosine compounds are selected from sphingosine, phytosphingosine, or dihydrosphingosine.

2. The cream as described in claim 1, characterized in that, The composition comprises the following components in parts by weight: 1.5-3 parts of ceramide from palm fruit oil, 0.2-0.4 parts of squalene, and 0.05-0.08 parts of rusco saponin.

3. The cream as described in claim 1 or 2, characterized in that, The cream also contains commonly used cosmetic excipients, including emulsifiers, oils, polyols, thickeners, preservatives, chelating agents, and solvents.

4. The cream as described in claim 1 or 2, characterized in that, The fatty acids in the Mauritius fruit oil are obtained from Mauritius fruit oil through a saponification reaction.

5. The cream as described in claim 1, characterized in that, The cream contains at least the following components in parts by weight: The ingredients are: 0.1-5 parts ceramide from mulberry fruit oil, 0.05-0.5 parts squalene, 0.01-0.1 parts rusecosanoside, 0.3-5.0 parts emulsifier, 5.0-20.0 parts oil, 2.0-10.0 parts polyol, 0.1-1.0 parts thickener, 0.3-1.5 parts preservative, 0.05-0.20 parts chelating agent, and 90-100 parts water.

6. The cream as described in claim 5, characterized in that, The cream contains at least the following components in parts by weight: The ingredients are: 1.5-3 parts ceramide from mulberry fruit oil, 0.2-0.4 parts squalene, 0.05-0.08 parts rusecoside, 2.0-5.0 parts emulsifier, 6.0-12.0 parts oil, 3.0-8.0 parts polyol, 0.5-1.0 parts thickener, 0.3-0.8 parts preservative, 0.05-0.15 parts chelating agent, and 93-98 parts water.

7. A method for preparing a cream containing ceramide from palmetto fruit oil according to any one of claims 1-6, characterized in that, The preparation method includes: S1. Preparation of the aqueous phase: Mix polyol, thickener, chelating agent, aqueous emulsifier and water, heat to 75-80℃ and stir to dissolve; S2. Preparation of the oil phase: Heat the oil phase emulsifier and oil to 70-75℃ to melt. After complete melting, add ceramide from morrhagic fruit oil and squalene before phase combination, and stir until the mixture is uniformly melted. S3. Homogenize the aqueous and oil phases; after cooling, add Ruscosaponin. S4: Cool to 65-70℃, add preservative, stir to dissolve, then stir and cool to about 38℃ to obtain the product.

8. The method for preparing the cream as described in claim 7, characterized in that, S3. Homogenize the aqueous and oil phases together. After cooling, add rusco saponin; the rusco saponin is pre-dissolved by heating butylene glycol to 85°C and stirring before being added.

9. The use of the cream containing ceramide from the fruit oil of *Pterocarya stenoptera* according to any one of claims 1-6 in the preparation of cosmetics.

10. A cosmetic product, characterized in that, The cosmetic product comprises at least the cream containing ceramide from palm fruit oil as described in any one of claims 1-6.

11. The cosmetic product as described in claim 10, characterized in that, The cosmetics in question are skincare products.

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