Scalp care composition and application thereof in preparation of oxidized hair dye

Abstract

The invention belongs to the technical field of daily chemical products, and particularly relates to a scalp care composition and application thereof in preparation of an oxidized hair dye. The nigella glandulifera seed extract, the white perilla leaf extract and the tremella polysaccharide are used as active components and have a synergistic effect, so that the moisturizing, anti-inflammatory and anti-oxidation effects of the composition are remarkably improved, and the problems that an existing scalp care composition is poor in moisturizing, anti-inflammatory and anti-oxidation effects and high in irritation can be solved. Wherein the preparation of the nigella glandulifera seed extract adopts an ultrasonic-assisted deep-eutectic solvent extraction technology, the preparation of a deep-eutectic solvent adopts thiobetaine, caffeic acid and xylitol, and the nigella glandulifera seed extract has the advantages of being green, safe, high in extraction efficiency, convenient to operate and designable. The oxidized hair dye provided by the invention comprises the scalp care composition provided by the invention, so that the damage of the hair dye to the scalp is reduced, and the oxidized hair dye has a relatively good market value.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical products, and particularly relates to a scalp care composition and application thereof in preparing an oxidative hair dye. Background Art

[0002] Hair dyes are cosmetics used to change hair color and beautify the hair. They are categorized by duration of effect as temporary, semi-permanent, or permanent. Permanent, or oxidative, hair dyes are the most important category of hair dyes, providing effective, permanent color. Dye molecules penetrate directly into the hair cortex and are oxidized by a developer to form insoluble, colored condensation macromolecules that become trapped within the hair, giving the hair its color. Oxidative hair dyes are composed of dye intermediates, coupling agents, alkalizing agents, and oxidizing agents.

[0003] Oxidative hair dyes can irritate and damage the hair and scalp. The scalp barrier is composed of the sebum membrane and the stratum corneum. The sebum membrane is a watery membrane covering the surface of the skin and is the outermost layer of the skin barrier, locking in moisture and providing anti-inflammatory properties. When the scalp barrier is damaged, scalp permeability increases, leading to severe water loss, which can cause scalp inflammation and hair follicle inflammation. Existing scalp care products primarily moisturize the scalp by adding moisturizers such as polyols, plant polysaccharide extracts, or protein hydrolyzates to achieve scalp moisturization, strengthen hair roots, and reduce frizz and tangles. For scalp inflammation, conventional soothing ingredients such as dipotassium glycyrrhizate and bisabolol are often used, ensuring the safety of the ingredients.

[0004] However, scalp care products currently on the market still suffer from numerous issues, such as greasiness, stickiness, white flakes, and weak anti-inflammatory properties, failing to meet the needs for scalp moisturization and anti-inflammatory benefits. Therefore, there is an urgent need to develop a scalp care composition with excellent anti-inflammatory and antioxidant properties that is mild and non-irritating and suitable for use in the preparation of oxidative hair dyes. Summary of the Invention

[0005] In response to the above-mentioned defects or improvement needs of the prior art, the present invention provides a scalp care composition and its use in the preparation of an oxidative hair dye, which solves the problems of the existing scalp care composition having poor anti-inflammatory and antioxidant effects and strong irritation.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a scalp care composition, comprising the following components in parts by weight: 12-16 parts of black cumin seed extract, 9-12 parts of perilla leaf extract, and 7-9 parts of tremella polysaccharide.

[0008] Black cumin seeds contain a variety of biologically active ingredients, mainly including oils, volatile oils, saponins, flavonoids, alkaloids, etc., which have anti-tumor, anti-inflammatory, antioxidant, anti-diabetic, lipid-lowering and blood pressure-lowering effects.

[0009] White basil leaf extract contains rich bioactive substances, such as perillaldehyde, perillyl alcohol, rosmarinic acid, flavonoids, etc., which have excellent antioxidant, anti-inflammatory, anti-cardiovascular disease, anti-tumor and anti-cancer effects.

[0010] Tremella polysaccharide is a water-soluble polysaccharide, which is mainly derived from the fruiting bodies, spores, fermentation broth and cell walls of Tremella. The main chain is composed of mannose, xylose and glucuronic acid connected by α-1,3-glycosidic bonds, and the side chain is composed of galactose, arabinose and fucose. It has moisturizing, anti-oxidation, anti-inflammatory and immune-enhancing effects.

[0011] Preferably, the composition comprises the following components in parts by weight: 14 parts of black cumin seed extract, 11 parts of perilla leaf extract, and 8 parts of tremella polysaccharide.

[0012] Preferably, the preparation method of the black cumin seed extract comprises the following steps:

[0013] A. Grind dried black cumin seeds and pass through an 80-mesh sieve to obtain black cumin seed powder;

[0014] B. Mixing sulfobetaine, caffeic acid, and xylitol uniformly, adding deionized water, and mixing uniformly to obtain a deep eutectic solvent;

[0015] C. Adding black cumin seed powder to a deep eutectic solvent for ultrasonic-assisted extraction, and collecting the supernatant after centrifugation to obtain the black cumin seed extract.

[0016] Deep eutectic solvents (DES) are homogeneous mixtures primarily composed of hydrogen bond acceptors (such as quaternary ammonium salts) and hydrogen bond donors (such as polyols, organic acids, and sugars) mixed in a specific molar ratio. Through intermolecular hydrogen bonding, they form a uniform, stable liquid that can be used to extract active ingredients such as flavonoids, phenols, saponins, polysaccharides, and terpenes from organisms. As a new, environmentally friendly, green solvent, DES offers advantages such as biodegradability, high thermal stability, safety and non-toxicity, high biocompatibility, and strong solubility and penetration. Furthermore, its raw materials are widely available and economical, its preparation method is simple, and it is designable. Its properties can be adjusted by selecting appropriate composition and ratios, making it a promising alternative to traditional organic extraction solvents.

[0017] Sulfobetaine is a betaine derivative that acts as a hydrogen bond acceptor and has the advantages of biocompatibility and high solubility.

[0018] Caffeic acid is a natural phenolic acid compound that acts as a hydrogen bond donor, is biocompatible and environmentally friendly, and can improve reaction efficiency.

[0019] Xylitol, as a hydrogen bond donor, can adjust the viscosity of the deep eutectic solvent due to its polyol properties, so that it maintains fluidity, thereby improving the extraction efficiency.

[0020] Further preferably, in step B, the molar ratio of sulfobetaine, caffeic acid and xylitol is 1:1-3:2-4.

[0021] More preferably, the molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:2:3.

[0022] Further preferably, the total mass concentration of sulfobetaine, caffeic acid and xylitol in the deep eutectic solvent of step B is 50%-70%.

[0023] Further preferably, in step C, the solid-liquid ratio of black cumin seed powder to deep eutectic solvent is 1 g: 20-30 mL.

[0024] Further preferably, in step C, the ultrasonic power is 30-50 kHz, the ultrasonic temperature is 40-60° C., and the ultrasonic-assisted extraction time is 25-45 min.

[0025] In a second aspect, the present invention provides an oxidative hair dye, comprising a first agent and a second agent;

[0026] The first agent comprises the following raw materials in weight percentage: 18-22% of the scalp care composition of the first aspect, 5-10% of an alkaline agent, 1-5% of a dye intermediate, 0.5-2.5% of a coupling agent, 2-5% of a moisturizing agent, 0.1-0.3% of a preservative, 1-3% of a hair nourishing agent, 2-4% of an emulsifier, 0.2-0.5% of an antioxidant, 2-4% of a film-forming agent, 0.1-0.3% of a chelating agent, 0.4-0.6% of a fragrance, and the balance of deionized water;

[0027] The second agent comprises the following raw materials in percentage by weight: 2-6% oxidant, 1-3% moisturizer, 1-3% hair nutrition agent, 1-2% emulsifier, 0.01-0.1% pH regulator, 0.4-0.6% essence, and the balance deionized water.

[0028] Preferably, the alkaline agent in the first agent is ethanolamine or ammonium hydroxide;

[0029] The dye intermediate is one of m-phenylenediamine or o-phenylenediamine;

[0030] The coupling agent is at least one of m-aminophenol, 4-chlororesorcinol, resorcinol and catechol;

[0031] The moisturizing agent is at least one of glycerin, panthenol, sodium hyaluronate, petrolatum, lauric acid, myristic acid, palmitic acid, butylene glycol, propylene glycol and glyceryl stearate;

[0032] The preservative is at least one of phenoxyethanol, sodium benzoate, sodium lactate, p-hydroxyacetophenone, methylparaben, propylparaben and ethylhexylglycerin;

[0033] The hair nutrient is at least one of hydrolyzed wheat protein, ginseng extract, Polygonum multiflorum extract, aloe extract, camellia seed oil, grape seed oil and jojoba seed oil;

[0034] The emulsifier is at least one of ceteareth-25, ceteareth-30, ceteareth-40 and glyceryl stearate;

[0035] The antioxidant is at least one of sodium erythorbate, sodium sulfite, ascorbic acid, sodium ascorbate, and sodium bisulfite;

[0036] The film-forming agent is at least one of cyclopentasiloxane, polydimethylsiloxane, chitosan, hyaluronic acid, polyquaternium-7, polyquaternium-10 and trimethylsiloxysilane;

[0037] The chelating agent is at least one of etidronate, disodium EDTA and tetrasodium EDTA.

[0038] Preferably, the oxidant in the second agent is at least one of hydrogen peroxide, ammonium persulfate, potassium persulfate, sodium persulfate, sodium bromate, potassium bromate, sodium peroxide, potassium peroxide and ammonium persulfate;

[0039] The moisturizing agent is at least one of petrolatum, lauric acid, myristic acid, palmitic acid, shea butter, glycerin and stearic acid glyceryl ester;

[0040] The hair nutrient is at least one of gentian extract, wheat amino acids, soybean amino acids, arginine, serine and threonine;

[0041] The emulsifier is at least one of cetearyl alcohol, ceteareth-25 and glyceryl stearate;

[0042] The pH regulator is at least one of disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, citric acid, tartaric acid and lactic acid.

[0043] Beneficial effects of the present invention:

[0044] 1) The present invention provides a scalp care composition, comprising a black cumin seed extract, a perilla leaf extract, and a tremella polysaccharide; wherein the black cumin seed extract, the perilla leaf extract, and the tremella polysaccharide are active ingredients, and the three ingredients have a synergistic effect, so that the moisturizing, anti-inflammatory, and antioxidant effects of the composition are significantly improved, which can solve the problem that existing scalp care compositions have poor moisturizing, anti-inflammatory, and antioxidant effects and are highly irritating.

[0045] 2) The present invention uses ultrasound-assisted deep eutectic solvent extraction to extract black cumin seeds, wherein the preparation of the deep eutectic solvent is selected from sulfobetaine, caffeic acid and xylitol, which has the advantages of being green and safe, having high extraction efficiency, easy operation and designability.

[0046] 3) The present invention provides an oxidative hair dye, which includes the scalp care composition provided by the present invention, reduces damage to the scalp caused by the hair dye and has good market value. DETAILED DESCRIPTION

[0047] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined purpose of the invention, the following is a detailed description of the specific implementation methods, structures, features and effects of the present invention in combination with the composition.

[0048] For the following compositions, experimental methods where specific conditions are not specified were generally performed under conventional conditions or those recommended by the manufacturer. Materials and reagents used were commercially available unless otherwise specified.

[0049] Some of the raw materials and their sources are as follows:

[0050] Black cumin seeds were purchased from Bozhou Ruixuetang Biotechnology Co., Ltd.

[0051] Perilla frutescens leaf extract was purchased from Lanzhou Waterless Biotechnology Co., Ltd.

[0052] Tremella polysaccharide was purchased from Shanxi Xinnuo Biotechnology Co., Ltd.

[0053] The preparation method of black cumin seed extract ① comprises the following steps:

[0054] A. Grind dried black cumin seeds and pass through an 80-mesh sieve to obtain black cumin seed powder;

[0055] B. Mixing sulfobetaine, caffeic acid, and xylitol uniformly, adding deionized water, and mixing uniformly to obtain a deep eutectic solvent;

[0056] C. adding black cumin seed powder to a deep eutectic solvent for ultrasonic-assisted extraction, and collecting the supernatant after centrifugation to obtain the black cumin seed extract;

[0057] Wherein, the molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:2:3;

[0058] The total mass concentration of sulfobetaine, caffeic acid and xylitol in the deep eutectic solvent of step B is 60%;

[0059] In step C, the solid-liquid ratio of black cumin seed powder to deep eutectic solvent is 1 g: 25 mL;

[0060] In step C, the ultrasonic power is 40 kHz, the ultrasonic temperature is 50° C., and the ultrasonic-assisted extraction time is 35 minutes.

[0061] The preparation method of black cumin seed extract ② comprises the following steps:

[0062] A. Grind dried black cumin seeds and pass through an 80-mesh sieve to obtain black cumin seed powder;

[0063] B. Mixing sulfobetaine, caffeic acid, and xylitol uniformly, adding deionized water, and mixing uniformly to obtain a deep eutectic solvent;

[0064] C. adding black cumin seed powder to a deep eutectic solvent for ultrasonic-assisted extraction, and collecting the supernatant after centrifugation to obtain the black cumin seed extract;

[0065] Wherein, the molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:1:2;

[0066] The total mass concentration of sulfobetaine, caffeic acid and xylitol in the deep eutectic solvent of step B is 70%;

[0067] In step C, the solid-liquid ratio of black cumin seed powder to deep eutectic solvent is 1 g: 20 mL;

[0068] In step C, the ultrasonic power is 30 kHz, the ultrasonic temperature is 40° C., and the ultrasonic-assisted extraction time is 45 minutes.

[0069] The preparation method of black cumin seed extract ③ comprises the following steps:

[0070] A. Grind dried black cumin seeds and pass through an 80-mesh sieve to obtain black cumin seed powder;

[0071] B. Mix sulfobetaine, caffeic acid and xylitol, add deionized water, and mix well to obtain a deep eutectic solvent;

[0072] C. adding black cumin seed powder to a deep eutectic solvent for ultrasonic-assisted extraction, and collecting the supernatant after centrifugation to obtain the black cumin seed extract;

[0073] Wherein, the molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:3:4;

[0074] The total mass concentration of sulfobetaine, caffeic acid and xylitol in the deep eutectic solvent of step B is 50%;

[0075] In step C, the solid-liquid ratio of black cumin seed powder to deep eutectic solvent is 1 g:30 mL;

[0076] In step C, the ultrasonic power is 50 kHz, the ultrasonic temperature is 60° C., and the ultrasonic-assisted extraction time is 25 minutes.

[0077] The only difference between the black cumin seed extract ④ and the black cumin seed extract ① is that caffeic acid is not added in the preparation step B of the black cumin seed extract ④, and the missing amount is supplemented by sulfobetaine and xylitol in a molar ratio of 1:3. Other conditions are the same as those of the black cumin seed extract ①.

[0078] The only difference between the black cumin seed extract ⑤ and the black cumin seed extract ① is that xylitol is not added in the preparation step B of the black cumin seed extract ⑤, and the missing amount is supplemented by sulfobetaine and caffeic acid in a molar mass ratio of 1:2. Other conditions are the same as those of the black cumin seed extract ①.

[0079] The only difference between the black cumin seed extract ⑥ and the black cumin seed extract ① is that the molar ratio of sulfobetaine, caffeic acid and xylitol in the preparation step B of the black cumin seed extract ⑥ is 1:0.5:4.5, and the other conditions are the same as those of the black cumin seed extract ①.

[0080] The only difference between the black cumin seed extract ⑦ and the black cumin seed extract ① is that the molar mass ratio of sulfobetaine, caffeic acid and xylitol in the preparation step B of the black cumin seed extract ⑦ is 1:3.5:1.5, and the other conditions are the same as those of the black cumin seed extract ①.

[0081] The components (parts by weight) of the scalp care compositions of Examples 1-3 of the present invention are shown in Table 1 below. During preparation, each component is weighed and stirred evenly according to the formula amount to obtain each composition.

[0082] Table 1 Components and weight percentages of the scalp care compositions of Examples 1-3

[0083]

[0084] Comparative Example 1: Compared with Example 1, the difference is that in Comparative Example 1, no black cumin seed extract is added, and the missing amount is supplemented by white osmanthus leaf extract and tremella polysaccharide in a weight ratio of 11:8. Other conditions are the same as in Example 1.

[0085] Comparative Example 2: Compared with Example 1, the difference is that in Comparative Example 2, no white frutescens leaf extract is added, and the missing amount is supplemented by black cumin seed extract and tremella polysaccharide in a weight ratio of 14:8. Other conditions are the same as in Example 1.

[0086] Comparative Example 3: Compared with Example 1, the difference is that no Tremella polysaccharide is added in Comparative Example 3, and the missing amount is supplemented by black cumin seed extract and white osmanthus leaf extract in a weight ratio of 14:11. Other conditions are the same as in Example 1.

[0087] Comparative Example 4: Compared with Example 1, the difference is that in Comparative Example 4, black cumin seed extract ④ is used instead of black cumin seed extract ①, and other conditions are the same as those in Example 1.

[0088] Comparative Example 5: Compared with Example 1, the difference is that in Comparative Example 5, black cumin seed extract ⑤ is used instead of black cumin seed extract ①, and other conditions are the same as those in Example 1.

[0089] Comparative Example 6: Compared with Example 1, the difference is that in Comparative Example 6, black cumin seed extract ⑥ is used instead of black cumin seed extract ①, and other conditions are the same as those in Example 1.

[0090] Comparative Example 7: Compared with Example 1, the difference is that in Comparative Example 7, black cumin seed extract ⑦ is used instead of black cumin seed extract ①, and other conditions are the same as those in Example 1.

[0091] Test Example 1 Anti-inflammatory effect test

[0092] TNF-α is an acute phase protein and one of the pro-inflammatory cytokines. It is involved in initiating and regulating inflammatory responses. Reducing its content can inhibit inflammatory responses, thereby achieving anti-inflammatory effects.

[0093] Test substances: scalp care compositions prepared in Examples 1-3 and Comparative Examples 1-7;

[0094] The specific test steps are as follows: macrophage RAW264.7 cells were seeded into 96 wells (1.0×10 4 The plates were incubated at 36°C with 5% CO2 for 18 hours. The original culture medium was discarded and the cells were washed three times with PBS buffer. After treatment with 5 μL of 1.5% (v / v) test substance diluted in water for 2 hours, PBS buffer containing 1 μg / mL LPS was added and stimulated for 1 hour to establish an inflammatory model. A control group 1 (no test substance, only LPS) and a control group 2 (no LPS, only test substance) were set up. The cell supernatant was collected and the TNF-α content was detected using an ELISA kit. The experiment was repeated three times, and the average value was calculated.

[0095] Calculate and record the TNF-α inhibition rate according to the following formula:

[0096]

[0097] Where:

[0098] A1 is the TNF-α content in control group 1;

[0099] A2 is the TNF-α content in control group 2;

[0100] S x is the TNF-α content in the test substance group;

[0101] The test results are shown in Table 2.

[0102] Table 2 Anti-inflammatory effect test results

[0103]

[0104] Test Example 2 Antioxidant Effect Test

[0105] Test substances: scalp care compositions prepared in Examples 1-3 and Comparative Examples 1-7;

[0106] The antioxidant properties of the scalp care compositions prepared in Examples 1-3 and Comparative Examples 1-7 were evaluated by testing the scavenging efficiency of DPPH;

[0107] The specific test method is as follows: take 1 mL of the test substance and add 10 mL of a concentration of 1×10 -4 mol / L DPPH solution, mix thoroughly, then place it at room temperature in the dark for 30 minutes, and immediately test the absorbance at 518 nm, record it as A X A control group was set up, with no test substance added and only a solution containing DPPH. The absorbance value was recorded as A0. The experiment was repeated 3 times and the average value was calculated.

[0108] The DPPH clearance rate was calculated as follows:

[0109]

[0110] Where:

[0111] A X is the absorbance value of the test substance group;

[0112] A0 is the absorbance value of the control group;

[0113] The test results are shown in Table 3.

[0114] Table 3 Antioxidant performance test results

[0115]

[0116] The test results are shown in Tables 2 and 3. The TNF-α inhibition rate and DPPH scavenging rate of the compositions prepared in Examples 1-3 were as high as 80% and 75%, respectively, indicating that they have excellent anti-inflammatory and antioxidant effects; the TNF-α inhibition rate and DPPH scavenging rate of the compositions prepared in Comparative Examples 1-7 were lower than those in Examples 1-3, indicating that the component composition of the composition and the specific preparation process conditions of the black cumin seed extract (such as the composition of the hydrogen bond donor in the deep eutectic solvent and the dosage ratio of the hydrogen bond acceptor and the hydrogen bond donor) will affect the anti-inflammatory and antioxidant effects of the composition.

[0117] Wherein, compared with Example 1, in Comparative Examples 1-3, one of black seed extract, white frutescens leaf extract and white fungus polysaccharide is not added respectively, and the TNF-α inhibition rate and DPPH clearance rate of the composition prepared by Comparative Examples 1-3 are both lower than those of Example 1, indicating that there is a synergistic anti-inflammatory and antioxidant effect between black seed extract, white frutescens leaf extract and white fungus polysaccharide. Compared with Example 1, in Comparative Examples 4-5, the deep eutectic solvent is not added with caffeic acid and xylitol respectively, and the TNF-α inhibition rate and DPPH clearance rate of the composition prepared by Comparative Examples 4-5 are both lower than those of Example 1, indicating that there is a synergistic effect between caffeic acid, xylitol and sulfobetaine, and the composition of hydrogen bond donors directly affects the extraction effect; further, caffeic acid and xylitol are the deep eutectic solvents that the inventors have verified through multiple tests to be most suitable for extracting anti-inflammatory and antioxidant active substances in black seed. Compared with Example 1, the configuration of the deep eutectic solvent in Comparative Examples 6-7 all used different molar ratios of sulfobetaine, caffeic acid, and xylitol, and the TNF-α inhibition rate and DPPH clearance rate of the compositions prepared in Comparative Examples 6-7 were lower than those in Example 1, indicating that the molar ratio of sulfobetaine, caffeic acid, and xylitol in the deep eutectic solvent directly affects the extraction effect, thereby affecting the anti-inflammatory and antioxidant effects; further, the inventors found through multiple experimental studies that the optimal molar ratio of sulfobetaine, caffeic acid, and xylitol is 1:1-3:2-4.

[0118] In summary, the present invention not only optimizes the preparation method of black cumin seed extract, that is, extracting the black cumin seed extract by ultrasound-assisted deep eutectic solvent extraction, but also selects sulfobetaine, caffeic acid and xylitol when preparing the deep eutectic solvent, which has the advantages of being green and safe, having high extraction efficiency, being easy to operate and being designable; but also by using black cumin seed extract, perilla leaf extract and tremella polysaccharide as active ingredients, the three synergistically enhance the effect, so that the anti-inflammatory and antioxidant effects of the composition are significantly improved, which can solve the problem that the existing scalp care compositions have poor anti-inflammatory and antioxidant effects and strong irritation.

[0119] Application Examples

[0120] In this application example, the scalp care composition prepared in Example 1 is used to prepare an oxidative hair dye. The hair dye includes a first agent and a second agent, wherein the first agent includes the following raw materials in percentage by weight: 20% of the scalp care composition, 3% of ethanolamine, 4% of ammonium hydroxide, 3% of m-phenylenediamine, 1.5% of m-aminophenol, 0.5% of 4-chlororesorcinol, 0.5% of sodium hyaluronate, 0.5% of panthenol, 0.5% of glycerin, 0.5% of lauric acid, 0.5% of myristic acid, 0.5% of palmitic acid, 0.5% of glyceryl stearate, 0.1% of phenoxyethanol, 0.1% of ethylhexylglycerin, 0.2% of hydrolyzed wheat protein, 0.2% of ginseng extract, 0.2% of Polygonum multiflorum extract, 0.2% of aloe extract, 0.2% of camellia seed oil, 0.2% of grape seed oil, and 0.2% of jojoba seed oil. 2%, cetearyl alcohol polyether-252%, glyceryl stearate 2%, sodium isoascorbate 0.2%, sodium sulfite 0.2%, cyclopentasiloxane 3%, disodium EDTA 0.2%, flavor 0.5%, deionized water balance; the second dose includes the following raw materials in weight percentage: hydrogen peroxide 5%, petrolatum 0.4%, lauric acid 0.4%, myristic acid 0.4%, palmitic acid 0.4%, shea butter 0.4%, glycerol 0.4%, glyceryl stearate 0.4%, gentian extract 0.6%, arginine 0.6%, serine 0.6%, threonine 0.6%, cetearyl alcohol 0.5%, cetearyl alcohol polyether-25 0.5%, glyceryl stearate 0.5%, disodium hydrogen phosphate 0.04%, flavor 0.5%, deionized water balance.

[0121] Test Example 3 Evaluation of the effect of promoting proliferation of human dermal papilla cells

[0122] Human Hair Follicle Dermal Papilla Cells (HDPCs) are a special type of cell located at the base of hair follicles. They play a vital role in hair growth and hair follicle cycle regulation. Their proliferation helps maintain or restore hair follicle function, thus benefiting scalp health. Therefore, evaluating the proliferation effect of HDPCs can be used as an important indicator to measure the effectiveness of scalp care ingredients.

[0123] Test substance: Oxidative hair dye prepared in Application Example;

[0124] The test substance was diluted to 15% concentration with serum-free medium, filtered through a 0.22 μm filter membrane for sterilization and then used for standby. Human dermal papilla cells in logarithmic growth phase were taken and 4×10 3Each well of a 96-well plate was inoculated with 100 μL of DMEM medium supplemented with 10% fetal bovine serum. The plate was incubated in a carbon dioxide incubator at 37°C and 5% CO2 for 24 hours, the medium was discarded, and the experimental group was replaced with 100 μL of the test substance. The blank control group was added with 100 μL of high-glucose DMEM medium. Five replicate wells were set up for each group. After a further 48 hours of incubation, the test substance or medium was discarded, and a 10% WST-1 solution prepared in serum-free culture medium was added to each well. The cells were incubated for a further 4 hours. The absorbance was measured at a wavelength of 450 nm using a microplate reader and the results were recorded. The relative growth rate (RGR) was the ratio of the average absorbance of the experimental group to the absorbance of the blank control group.

[0125] The results are shown in Table 4. The relative proliferation rate of the test group was significantly higher than that of the blank control group, indicating that the oxidative hair dye prepared using the scalp care composition of the present invention has the effect of promoting the proliferation of human hair papilla cells, maintaining scalp health, and thus reducing the damage to the scalp caused by the oxidative hair dye.

[0126] Table 4 Results of the effect of promoting proliferation of human dermal papilla cells

[0127] Group Relative value-added rate (%) Blank control group 100.00 experimental group 128.07

[0128] The above description is merely a preferred composition of the present invention and does not constitute any form of limitation to the present invention. Although the present invention has been disclosed as a preferred composition as above, it is not intended to limit the present invention. Any person skilled in the art can make some changes or modifications to equivalent compositions using the technical contents disclosed above without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above compositions based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A scalp care composition, characterized in that The composition comprises the following components in parts by weight: 12-16 parts of black cumin seed extract, 9-12 parts of perilla leaf extract, and 7-9 parts of tremella polysaccharide; The preparation method of the black cumin seed extract comprises the following steps: A. Grind dried black cumin seeds and pass through an 80-mesh sieve to obtain black cumin seed powder; B. Mixing sulfobetaine, caffeic acid, and xylitol uniformly, adding deionized water, and mixing uniformly to obtain a deep eutectic solvent; C. Adding black cumin seed powder to a deep eutectic solvent for ultrasonic-assisted extraction, and collecting the supernatant after centrifugation to obtain the black cumin seed extract.

2. The composition according to claim 1, characterized in that The composition comprises the following components in parts by weight: 14 parts of black cumin seed extract, 11 parts of perilla leaf extract, and 8 parts of tremella polysaccharide.

3. The composition according to claim 1, characterized in that The molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:1-3:2-4.

4. The composition according to claim 3, characterized in that The molar ratio of sulfobetaine, caffeic acid and xylitol in step B is 1:2:

3.

5. The composition according to claim 1, characterized in that The total mass concentration of sulfobetaine, caffeic acid and xylitol in the deep eutectic solvent of step B is 50%-70%.

6. The composition according to claim 1, characterized in that In step C, the solid-liquid ratio of black cumin seed powder to deep eutectic solvent is 1 g: 20-30 mL.

7. The composition according to claim 1, characterized in that In step C, the ultrasonic power is 30-50 kHz, the ultrasonic temperature is 40-60° C., and the ultrasonic-assisted extraction time is 25-45 minutes.

8. An oxidative hair dye, characterized in that: The hair dye comprises a first agent and a second agent; The first agent comprises the following raw materials in weight percentage: 18-22% of the scalp care composition according to any one of claims 1 to 7, 5-10% of an alkaline agent, 1-5% of a dye intermediate, 0.5-2.5% of a coupling agent, 2-5% of a moisturizing agent, 0.1-0.3% of a preservative, 1-3% of a hair nourishing agent, 2-4% of an emulsifier, 0.2-0.5% of an antioxidant, 2-4% of a film-forming agent, 0.1-0.3% of a chelating agent, 0.4-0.6% of a fragrance, and the balance of deionized water; The second agent comprises the following raw materials in percentage by weight: 2-6% oxidant, 1-3% moisturizer, 1-3% hair nutrition agent, 1-2% emulsifier, 0.01-0.1% pH regulator, 0.4-0.6% essence, and the balance deionized water.

9. The oxidative hair dye according to claim 8, characterized in that The alkaline agent in the first agent is ethanolamine and ammonium hydroxide; The dye intermediate is one of m-phenylenediamine or o-phenylenediamine; The coupling agent is at least one of m-aminophenol, 4-chlororesorcinol, resorcinol and catechol; The moisturizing agent is at least one of glycerin, panthenol, sodium hyaluronate, petrolatum, lauric acid, myristic acid, palmitic acid, butylene glycol, propylene glycol and glyceryl stearate; The preservative is at least one of phenoxyethanol, sodium benzoate, sodium lactate, p-hydroxyacetophenone, methylparaben, propylparaben and ethylhexylglycerin; The hair nutrient is at least one of hydrolyzed wheat protein, ginseng extract, Polygonum multiflorum extract, aloe extract, camellia seed oil, grape seed oil and jojoba seed oil; The emulsifier is at least one of ceteareth-25, ceteareth-30, ceteareth-40 and glyceryl stearate; The antioxidant is at least one of sodium erythorbate, sodium sulfite, ascorbic acid, sodium ascorbate, and sodium bisulfite; The film-forming agent is at least one of cyclopentasiloxane, polydimethylsiloxane, chitosan, hyaluronic acid, polyquaternium-7, polyquaternium-10 and trimethylsiloxysilane; The chelating agent is at least one of etidronate, disodium EDTA and tetrasodium EDTA.

10. The oxidative hair dye according to claim 8, characterized in that The oxidant in the second agent is at least one of hydrogen peroxide, ammonium persulfate, potassium persulfate, sodium persulfate, sodium bromate, potassium bromate, sodium peroxide, potassium peroxide and ammonium persulfate; The moisturizing agent is at least one of petrolatum, lauric acid, myristic acid, palmitic acid, shea butter, glycerin and stearic acid glyceryl ester; The hair nutrient is at least one of gentian extract, wheat amino acids, soybean amino acids, arginine, serine and threonine; The emulsifier is at least one of cetearyl alcohol, ceteareth-25 and glyceryl stearate; The pH regulator is at least one of disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, citric acid, tartaric acid and lactic acid.

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