Microfluidic module, microfluidic chip and microfluidic system

Abstract

The invention provides a microfluidic module, a flow channel is arranged in the module, in the flow channel, the radius of a cylindrical cavity is larger than the radius of a front-stage flow channel and the radius of a rear-stage flow channel, and the communicating position of the front-stage flow channel and the cylindrical cavity is located on the cavity wall of the cylindrical cavity. The communicating position of the rear-stage flow channel and the cylindrical cavity is located in the center area of the bottom face of the cylindrical cavity. The micro-fluidic module further comprises a magnetic bead type switch, in the magnetic bead type switch, the controllable magnet and the permanent magnet are located on the upper face and the lower face of the cylindrical cavity respectively, the first rubber ring is located on the bottom face of the cylindrical cavity, and the ball bead is arranged in the cylindrical cavity and located above the first rubber ring. Therefore, when the controllable magnet does not have magnetism, the ball tightly presses the first rubber ring under the adsorption of the permanent magnet, so that the magnetic bead type switch closes the flow channel, and when the controllable magnet generates magnetism, the ball moves upwards under the adsorption of the controllable magnet, so that the magnetic bead type switch opens the flow channel. The magnetic bead type switch is simple in structure, smaller in size and more beneficial to integration.

Description

Technical Field

[0001] The present application relates to the field of microfluidics, and in particular to a microfluidics module, a microfluidics chip, and a microfluidics system. Background Art

[0002] Microfluidics uses microchannels to process and manipulate tiny fluids. It can integrate basic elements such as sample preparation, reaction, separation, and detection in biology, chemistry, and medicine onto a micrometer-scale microfluidic disk. Microfluidic analyzers then automatically complete the entire analysis process, resulting in widespread application in in vitro diagnostics. Microfluidics, characterized by miniaturization and integration, is primarily implemented through microfluidic chips, where valves are primarily used to control the precise flow of liquid within the flow channels.

[0003] Currently, commonly used microfluidic valves mainly include pneumatic valves, mechanical valves, and the like. Mechanical valves compress the lower film through the rotation and movement of mechanical components, and use the deformation of the film to block the pipeline. Pneumatic valves require a layer of deformable film to be embedded in them. An external pump feeds gas into the valve through the gas flow channel in the airway chip. The film deforms due to the gas pressure, blocking the pipeline and realizing the opening and closing of the valve. At present, the volume of pneumatic valves and mechanical valves in the existing technology is still a bit large for microfluidic chips, which is not conducive to the miniaturization of microfluidic chips, that is, it is not conducive to improving the integration of microfluidic chips. Summary of the Invention

[0004] To this end, the present application provides a microfluidic module, a microfluidic chip, and a microfluidic system, which are more conducive to the miniaturization of the microfluidic chip, that is, more conducive to improving the integration of the microfluidic chip.

[0005] In a first aspect, the present application provides a microfluidic module comprising a liquid inlet and a liquid outlet, wherein a flow channel connecting the liquid inlet and the liquid outlet is provided inside the microfluidic module; The flow channel is divided into a front-stage flow channel, a cylindrical cavity, and a rear-stage flow channel that are connected in sequence; the radius of the cylindrical cavity is larger than the radius of the front-stage flow channel and the radius of the rear-stage flow channel; the connection point between the front-stage flow channel and the cylindrical cavity is located at the cavity wall of the cylindrical cavity, and the connection point between the rear-stage flow channel and the cylindrical cavity is located at the central area of the bottom surface of the cylindrical cavity; The microfluidic module also includes a magnetic bead switch, which includes a controllable magnet, a permanent magnet, a ball, and a first rubber ring; the controllable magnet and the permanent magnet are both embedded in the microfluidic module and are respectively located above and below the cylindrical cavity; the radius of the first rubber ring and the radius of the ball are both adapted to the radius of the cylindrical cavity, the first rubber ring is located on the bottom surface of the cylindrical cavity, and the ball is arranged in the cylindrical cavity and above the first rubber ring; When the controllable magnet is non-magnetic, the ball presses the first rubber ring under the adsorption of the permanent magnet, so that the magnetic bead switch closes the flow channel; when the controllable magnet generates magnetism, the ball moves upward under the adsorption of the controllable magnet, so that the magnetic bead switch opens the flow channel, wherein the magnetic force generated by the controllable magnet is greater than the magnetic force of the permanent magnet.

[0006] In some embodiments, the connection point between the front-stage flow channel and the cylindrical cavity is lower than the horizontal plane of the ball center; The liquid source of the front-stage flow channel is the liquid inlet, and the liquid source of the liquid outlet is the rear-stage flow channel; And / or, a controllable magnet groove is provided on the upper surface of the microfluidic module, and the controllable magnet is located in the controllable magnet groove.

[0007] In some embodiments, the upper end of the cylindrical cavity is configured to be open and communicate with the controllable magnet groove.

[0008] In some embodiments, a connection magnet groove is formed on the lower surface of the microfluidic module, and the microfluidic module further comprises a connection magnet located in the connection magnet groove; The connecting magnet is used to be adsorbed on the substrate of the microfluidic chip to fix the position of the microfluidic module on the substrate.

[0009] In some embodiments, a permanent magnet groove is provided on the lower surface of the microfluidic module, and the permanent magnet is located in the permanent magnet groove.

[0010] In some embodiments, the bottom of the microfluidic module is provided with a connection boss formed by protruding outward, and the bottom of the microfluidic module is also provided with a connection recess formed by recessing inward, and the connection boss and the connection recess are adapted in shape; The upper surface of the connecting boss is provided with a first rubber ring hole formed inwardly concave, and the surface of the connecting recess facing the substrate is provided with a second rubber ring hole formed inwardly concave; The liquid inlet and the liquid outlet are respectively provided in the central area of the first rubber ring hole and the second rubber ring hole; A second rubber ring is provided in the first rubber ring hole, the size of the second rubber ring is adapted to the first rubber ring hole, and the sum of the depth of the first rubber ring hole and the depth of the second rubber ring hole is adapted to the thickness of the second rubber ring; The second rubber ring is used to seal the flow channel and the flow channel in the other microfluidic module when the connecting boss abuts against the connecting recess in the other microfluidic module.

[0011] In some embodiments, the liquid inlet is provided in the first rubber ring hole, and the liquid outlet is provided in the second rubber ring hole; the liquid source of the front-stage flow channel is the liquid inlet, and the liquid source of the liquid outlet is the rear-stage flow channel.

[0012] In some embodiments, a positioning post is provided on the upper surface of the connecting boss, and a positioning hole adapted to the positioning post is provided on the surface of the connecting recess facing the substrate.

[0013] In a second aspect, the present application provides a microfluidic chip comprising the microfluidic module as described in any one of the first aspects.

[0014] In a third aspect, the present application provides a microfluidic system, comprising the microfluidic chip as described in the second aspect.

[0015] Based on the above technical solution, it can be seen that compared with the existing technology, the magnetic bead switch in this application has a simple structure and a small size, which is more conducive to the miniaturization of the microfluidic chip, that is, it is more conducive to improving the integration of the microfluidic chip. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without any creative work.

[0017] Figure 1 This is a schematic diagram of the structure of the microfluidic module in an embodiment of the present application at one viewing angle; Figure 2 This is a schematic diagram of the structure decomposition of the controllable magnet and the controllable magnet groove in the embodiment of the present application; Figure 3 This is a schematic structural diagram of the microfluidic module in another embodiment of the present application; Figure 4 Schematic diagram of the structural decomposition of the permanent magnet, permanent magnet groove, connecting magnet and connecting magnet groove in the embodiment of the present application; Figure 5 When the magnetic bead switch is closed Figure 1 XX section diagram in; Figure 6 When the magnetic bead switch is open Figure 1 XX section diagram in; Figure 7 for Figure 1 Enlarged view of point A in the middle; Figure 8 yes Figure 7 The schematic diagram of the structure after the second rubber ring is decomposed; Figure 9 for Figure 3 Enlarged view of point B in the middle; Figure 10 A partial cross-sectional schematic diagram of the connection boss of the microfluidic module in the embodiment of the present application abutting against the connection recess in another microfluidic module; Figure 11 for Figure 10 Enlarged view of point C in the middle; Figure 12 yes Figure 10 The enlarged image of D is shown in the figure.

[0018] Description of reference numerals: 100. Microfluidic module; 110, liquid inlet; 120, liquid outlet; 130, flow channel; 140, magnetic bead switch; 150, connecting magnet groove; 160, connecting boss; 170, connecting recess; 131, front-stage flow channel; 132, cylindrical cavity; 133, rear-stage flow channel; 141. Controllable magnet; 142. Permanent magnet; 143. Ball; 144. First rubber ring; 145. Controllable magnet groove; 146. Permanent magnet groove; 151. Connecting magnets; 161, first rubber ring hole; 162, second rubber ring; 163, positioning column; 171. Second rubber ring hole; 172. Positioning hole. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are part of the embodiments of this application, not all of them. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0020] It should be understood that the terms used in this specification are only for the purpose of describing specific embodiments and are not intended to limit the present application. As used in this specification and the appended claims, the singular forms "a", "an", and "the" are intended to include the plural forms unless the context clearly indicates otherwise.

[0021] It should also be understood that the terms "first", "second", "third", "fourth", etc. (if any) in the description, claims or above-mentioned drawings of this application are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence, and cannot be understood as indicating or implying their relative importance or implicitly indicating the number of technical features indicated.

[0022] It should be further understood that the term "and / or" used in this specification and the appended claims refers to and includes any and all possible combinations of one or more of the associated listed items.

[0023] A microfluidic module 100 provided in an embodiment of the present application can be applied to a microfluidic chip. For example, the microfluidic chip can be an organoid flux cultivation chip, a cell high-throughput cultivation chip, an organoid interaction chip, a cell interaction chip, an organoid drug screening chip, a cell drug screening chip, a DNA information storage / extraction chip, and the like. It is understandable that the microfluidic chip 1000 may include other types of microfluidic modules in addition to the microfluidic module 100 provided in the embodiment of the present application. It should also be noted that the modules in the microfluidic chip are usually fixed on a substrate.

[0024] like Figure 1-12 As shown, the microfluidic module 100 includes a liquid inlet 110 , a liquid outlet 120 , a flow channel 130 disposed therein connecting the liquid inlet 110 and the liquid outlet 120 , and a magnetic bead switch 140 .

[0025] like Figure 5 and Figure 6 As shown, the flow channel 130 is divided into a front-stage flow channel 131, a cylindrical cavity 132, and a rear-stage flow channel 133, which are connected in sequence. It should be noted that the front and back here are relative to the cylindrical cavity 132, and do not limit the flow direction of the liquid in the flow channel 130. That is, the flow direction of the liquid in the flow channel 130 can be, in one embodiment, from the front-stage flow channel 131 to the cylindrical cavity 132, and then to the rear-stage flow channel 133. In this case, the source of the liquid in the front-stage flow channel 131 is the liquid inlet 110, and the source of the liquid in the liquid outlet 120 is the rear-stage flow channel 133. Of course, the opposite is also possible in other embodiments. The radius of the cylindrical cavity 132 is larger than the radius of the front-stage flow channel 131 and the radius of the rear-stage flow channel 133. In addition, the connection between the front-stage flow channel 131 and the cylindrical cavity 132 is located at the cavity wall of the cylindrical cavity 132, and the connection between the rear-stage flow channel 133 and the cylindrical cavity 132 is located at the center area of the bottom surface of the cylindrical cavity 132. This structural setting is mainly for the implementation of the magnetic bead switch 140. Therefore, if Figure 5 、 Figure 6 and Figure 12 As shown, the magnetic bead switch 140 includes a controllable magnet 141 , a permanent magnet 142 , a ball 143 and a first rubber ring 144 .

[0026] The controllable magnet 141 and the permanent magnet 142 are both embedded in the microfluidic module 100 and are located above and below the cylindrical cavity 132, respectively. For example, the controllable magnet 141, the permanent magnet 142 and the cylindrical cavity 132 can be as follows: Figure 5 The coaxial arrangement shown. It should be noted that the present embodiment does not restrict the method by which the controllable magnet 141 generates magnetic force. For example, a current coil may be provided within the controllable magnet 141 near the cylindrical cavity 132. When the current coil is energized, it generates magnetic force, and when the current is de-energized, it loses its magnetic force. The permanent magnet 142 is an object that generally maintains its magnetic properties.

[0027] The radius of the first rubber ring 144 and the radius of the ball 143 are both adapted to the radius of the cylindrical cavity 132. The first rubber 144 is located on the bottom surface of the cylindrical cavity 132, and the ball 143 is arranged in the cylindrical cavity 132 and is located above the first rubber ring 144. Such a structural setting is mainly for the reliability of the switching function of the magnetic bead switch 140. For example, since the ball 143 and the first rubber ring 144 are arranged in the cylindrical cavity 132 and will come into contact with the liquid during the application process, the ball 143 and the first rubber ring 144 can be made of corrosion-resistant materials. For example, the ball 143 can be a steel ball, and the first rubber ring 144 can be a rubber ring. In addition, the shape of the first rubber ring 144 can refer to Figure 7 The second rubber ring 162 in the.

[0028] Based on this, Figure 5 and Figure 12 As shown, when the controllable magnet 141 is demagnetized, the ball 143, attracted by the permanent magnet 142, presses against the first rubber ring 144, causing the magnetic bead switch 140 to shut off the flow channel 130. Specifically, when the controllable magnet 141 is demagnetized, the ball 143, attracted by the permanent magnet 142, tends to move downward. At the same time, because the ball 143, the first rubber ring 144, and the cylindrical cavity 132 are compatible in shape, the ball 143 presses against the first rubber ring 144, forming a seal that prevents liquid from passing through. In other words, the magnetic bead switch 140 closes the flow channel 130.

[0029] like Figure 6 As shown, when the controllable magnet 141 generates magnetism, and the magnetic force generated by the controllable magnet 141 is greater than the magnetic force of the permanent magnet 142, the ball 143 moves upward under the attraction of the controllable magnet 141. For example, the ball 143 moves upward until it abuts other components, causing the magnetic bead switch 140 to open the flow channel 130. Specifically, when the controllable magnet 141 generates magnetism, because its magnetic force is greater than the magnetic force of the permanent magnet 142, of course, the magnetic force of the controllable magnet 141 is greater than the magnetic force of the permanent magnet 142 plus the weight of the ball 143. However, because this is a micro device, the weight of the ball 143 can be ignored in this scenario, as long as the degree of greater than the first rubber ring 144 is sufficient to meet the technical objectives of the embodiments of the present application. Therefore, the ball 143 will move upward under the attraction of the controllable magnet 141, no longer pressing on the first rubber ring 144, and the liquid can now flow normally, that is, the magnetic bead switch 140 opens the flow channel 130.

[0030] For example, when the liquid source of the front-stage flow channel 131 is the liquid inlet 110 and the liquid source of the liquid outlet 120 is the rear-stage flow channel 133, when the controllable magnet 141 is non-magnetic, the ball 143, attracted by the permanent magnet 142, presses the first rubber ring 144, preventing the liquid in the front-stage flow channel 131 from flowing into the rear-stage flow channel 133. When the controllable magnet 141 is magnetized, the ball 143 moves upward under the attraction of the controllable magnet 141, and the liquid in the front-stage flow channel 131 can flow into the rear-stage flow channel 133.

[0031] In summary, compared with the prior art, the magnetic bead switch 140 in the embodiment of the present application has a simple structure and a small size, which is more conducive to the miniaturization of the microfluidic chip, that is, more conducive to improving the integration of the microfluidic chip.

[0032] In some embodiments, as Figure 5 and Figure 6 As shown, the connection between the front flow channel 131 and the cylindrical cavity 132 is lower than the horizontal plane of the ball 143, that is, the connection is higher than the first rubber ring 144, but lower than the horizontal plane including the ball 143 center. Figure 6 In the open state shown, the liquid flows very smoothly and does not flow into the space above the ball 143, so there is basically no liquid accumulation. That is, the magnetic bead switch 140 in the embodiment of the present application can be said to be a zero dead volume switch, while most of the pneumatic valves and electric valves in the prior art will have liquid accumulation during use.

[0033] In some embodiments, as Figure 2 As shown, the upper surface of the microfluidic module 100 is provided with a controllable magnet groove 145, and the controllable magnet 141 is located in the controllable magnet groove 145. It can be seen that the embodiment of the present application exposes the controllable magnet 141 on the upper surface of the microfluidic module 100, which makes it easier for an external control system to control whether the controllable magnet 141 has magnetic force. Exemplarily, the controllable magnet 141 can include a current coil, so that the power interface of the current coil can be set on the upper surface of the controllable magnet 141 and exposed, which is convenient for the control of the external control system.

[0034] In some embodiments, as Figure 2 and Figure 6 As shown, the upper end of the cylindrical cavity 132 is configured to be open and communicate with the controllable magnet groove 145. This maximizes the adsorption force of the controllable magnet 141 on the ball 143, while also facilitating the installation and subsequent maintenance of the ball 143 and the first rubber ring 144, thereby enhancing the user experience.

[0035] In some embodiments, as Figure 4As shown, the lower surface of the microfluidic module 100 is provided with a connecting magnet groove 150, and the microfluidic module 100 also includes a connecting magnet 151 located in the connecting magnet groove 150. The connecting magnet 151 is used to be adsorbed on the substrate of the microfluidic chip to fix the position of the microfluidic module 100 on the substrate. It can be seen that the embodiment of the present application adopts a magnetic suction method to achieve the fixation of the microfluidic module 100, and the operation is more flexible. In the prior art, some microfluidic modules are fixed by using intermediate connectors such as screws, which is not only inflexible, but also requires operators to be proficient in using professional tools such as torque screwdrivers. For example, the number of connecting magnets 151 can be reasonably set, such as two, four, etc., and a symmetrical arrangement is adopted.

[0036] In some embodiments, as Figure 4 As shown, a permanent magnet groove 146 is formed on the lower surface of the microfluidic module 100, and a permanent magnet 142 is located within the permanent magnet groove 146. While the previous embodiments employed magnetic attraction to secure the microfluidic module 100, the permanent magnet 142 is exposed in this embodiment because it is disposed beneath the cylindrical cavity 132. This allows the permanent magnet 142 to enhance the overall attraction force when the connecting magnet 151 is attached to the substrate, resulting in greater stability and reliability.

[0037] In some embodiments, as Figure 2 and Figure 7 As shown, the bottom of the microfluidic module 100 is provided with a connecting boss 160 protruding outward, as shown in FIG. Figure 3 and Figure 9 As shown, the bottom of the microfluidic module 100 is also provided with a connection notch 170 formed by an inward depression. Figure 1 、 Figure 3 and Figure 10 As shown, the connecting projection 160 and the connecting recess 170 are matched in shape.

[0038] like Figure 8 As shown, the upper surface of the connecting boss 160 is provided with a first rubber ring hole 161 formed inwardly concave. Figure 9 As shown, a second, inwardly recessed, rubber ring hole 171 is provided on the surface of the connection recess 170 facing the substrate, and the first rubber ring hole 161 and the second rubber ring hole 171 are adapted to each other. The liquid inlet 110 and the liquid outlet 120 are located in the center of the first rubber ring hole 161 and the second rubber ring hole, respectively.

[0039] The second rubber ring 162 is provided in the first rubber ring hole 161, and the size of the second rubber ring 162 is adapted to the first rubber ring hole 161. Figure 10 and Figure 11The sum of the depth of the first rubber ring hole 161 and the depth of the second rubber ring hole 171 is adapted to the thickness of the second rubber ring 162. For example, the thickness of the second rubber ring 162 is slightly greater than the sum of the depth of the first rubber ring hole 161 and the depth of the second rubber ring hole 171. Figure 10 Two identical microfluidic modules are used for illustration purposes only.

[0040] Based on this, Figure 10 and Figure 11 As shown, the second rubber ring 162 is used to seal the flow channel of the microfluidic module 100 in the embodiment of the present application and the flow channel in other microfluidic modules when the connection boss 170 of the microfluidic module 100 in the embodiment of the present application abuts against the connection recess in other microfluidic modules, so that there will be no leakage when the liquid flows in the microfluidic module 100 in the embodiment of the present application and the other microfluidic modules.

[0041] As discussed above, magnetic attraction can be used to secure the microfluidic module 100, providing greater flexibility. To further enhance flexibility, the present embodiment allows the microfluidic module 100 to be connected to other microfluidic modules, i.e., to connect the liquid inlet and outlet ports and achieve a seal, when magnetically attracted to the substrate.

[0042] It can be understood that the process of the microfluidic module 100 being adsorbed onto the substrate is roughly moving vertically toward the substrate. To this end, the embodiment of the present application provides a pair of snap-on structures, namely, a connecting boss 160 and a connecting recess 170, to facilitate connection. For example, assuming that the first microfluidic module has been magnetically fixed on the substrate, the connecting recess 170 of the microfluidic module 100 in the embodiment of the present application can be aligned with the connecting boss of the first microfluidic module, and then the microfluidic module 100 can be adsorbed onto the substrate so that the connecting recess 170 of the microfluidic module 100 abuts against the connecting boss of the first microfluidic module. Thereafter, assuming that the second microfluidic module needs to be magnetically adsorbed onto the substrate, similarly, after the adsorption is completed, the connecting boss 160 of the microfluidic module 100 abuts against the connecting recess of the second microfluidic module.

[0043] Furthermore, in order to achieve the sealing of the liquid inlet and outlet after the connecting boss and the connecting recess abut against each other, the embodiment of the present application adopts the first rubber ring hole 161, the second rubber ring hole 171 and the second rubber ring 162 mentioned above. In this way, when the connecting boss and the connecting recess abut against each other, the two will form a tight pressure on the second rubber ring 162, thereby achieving the technical purpose of sealing.

[0044] For example, the liquid inlet 110 can be provided in the first rubber ring hole 161, and the liquid outlet 120 can be provided in the second rubber ring hole 171. In addition, the liquid source of the front-stage flow channel 131 is the liquid inlet 110, and the liquid source of the liquid outlet 120 is the rear-stage flow channel 133, that is, Figure 5 or Figure 6 As shown.

[0045] In some embodiments, as Figure 7 and Figure 9 As shown, the upper surface of the connecting boss 160 is provided with a positioning post 163, and the surface of the connecting recess 170 facing the substrate is provided with a positioning hole 172 adapted to the positioning post 163. This facilitates the connection of the microfluidic module 100 with other microfluidic modules in the embodiment of the present application.

[0046] The present application also provides a microfluidic chip and a microfluidic system, wherein the microfluidic chip includes the microfluidic module 100 described in any of the above embodiments, and the microfluidic system includes the microfluidic chip. The specific implementation of the microfluidic chip and the microfluidic system is discussed above and will not be further described in detail in the present application.

[0047] The above description is merely a specific embodiment of the present application, but the scope of protection of the present application is not limited thereto. Any person skilled in the art can easily conceive of various equivalent modifications or substitutions within the technical scope disclosed in the present application, and such modifications or substitutions should be included in the scope of protection of the present application. Therefore, the scope of protection of the present application should be based on the scope of protection of the claims.

Claims

1. A microfluidic module, characterized in that: The microfluidic module comprises a liquid inlet and a liquid outlet, wherein a flow channel connecting the liquid inlet and the liquid outlet is provided inside the microfluidic module; The flow channel is divided into a front-stage flow channel, a cylindrical cavity, and a rear-stage flow channel that are connected in sequence; the radius of the cylindrical cavity is larger than the radius of the front-stage flow channel and the radius of the rear-stage flow channel; the connection point between the front-stage flow channel and the cylindrical cavity is located at the cavity wall of the cylindrical cavity, and the connection point between the rear-stage flow channel and the cylindrical cavity is located at the central area of the bottom surface of the cylindrical cavity; The microfluidic module also includes a magnetic bead switch, which includes a controllable magnet, a permanent magnet, a ball, and a first rubber ring; the controllable magnet and the permanent magnet are both embedded in the microfluidic module and are respectively located above and below the cylindrical cavity; the radius of the first rubber ring and the radius of the ball are both adapted to the radius of the cylindrical cavity, the first rubber ring is located on the bottom surface of the cylindrical cavity, and the ball is arranged in the cylindrical cavity and above the first rubber ring; When the controllable magnet is non-magnetic, the ball presses the first rubber ring under the adsorption of the permanent magnet, so that the magnetic bead switch closes the flow channel; when the controllable magnet generates magnetism, the ball moves upward under the adsorption of the controllable magnet, so that the magnetic bead switch opens the flow channel, wherein the magnetic force generated by the controllable magnet is greater than the magnetic force of the permanent magnet.

2. The microfluidic module according to claim 1, characterized in that The connection point between the front-stage flow channel and the cylindrical cavity is lower than the horizontal plane of the ball center; The liquid source of the front-stage flow channel is the liquid inlet, and the liquid source of the liquid outlet is the rear-stage flow channel; And / or, a controllable magnet groove is provided on the upper surface of the microfluidic module, and the controllable magnet is located in the controllable magnet groove.

3. The microfluidic module according to claim 2, characterized in that The upper end of the cylindrical cavity is configured to be open and communicated with the controllable magnet groove.

4. The microfluidic module according to any one of claims 1 to 3, characterized in that: A connecting magnet groove is formed on the lower surface of the microfluidic module, and the microfluidic module further comprises a connecting magnet located in the connecting magnet groove; The connecting magnet is used to be adsorbed on the substrate of the microfluidic chip to fix the position of the microfluidic module on the substrate.

5. The microfluidic module according to claim 4, characterized in that: A permanent magnet groove is provided on the lower surface of the microfluidic module, and the permanent magnet is located in the permanent magnet groove.

6. The microfluidic module according to claim 4, characterized in that: The bottom of the microfluidic module is provided with a connection boss formed by protruding outward, and the bottom of the microfluidic module is also provided with a connection recess formed by recessing inward, and the connection boss and the connection recess are adapted in shape; The upper surface of the connecting boss is provided with a first rubber ring hole formed inwardly concave, and the surface of the connecting recess facing the substrate is provided with a second rubber ring hole formed inwardly concave; The liquid inlet and the liquid outlet are respectively provided in the central area of the first rubber ring hole and the second rubber ring hole; A second rubber ring is provided in the first rubber ring hole, the size of the second rubber ring is adapted to the first rubber ring hole, and the sum of the depth of the first rubber ring hole and the depth of the second rubber ring hole is adapted to the thickness of the second rubber ring; The second rubber ring is used to seal the flow channel and the flow channel in the other microfluidic module when the connecting boss abuts against the connecting recess in the other microfluidic module.

7. The microfluidic module according to claim 6, characterized in that: The liquid inlet is arranged in the first rubber ring hole, and the liquid outlet is arranged in the second rubber ring hole; the liquid source of the front-stage flow channel is the liquid inlet, and the liquid source of the liquid outlet is the rear-stage flow channel.

8. The microfluidic module according to claim 6, characterized in that: A positioning column is provided on the upper surface of the connecting boss, and a positioning hole adapted to the positioning column is provided on the surface of the connecting recess facing the substrate.

9. A microfluidic chip, characterized in that: Comprising the microfluidic module according to any one of claims 1 to 8.

10. A microfluidic system, characterized in that: Comprising the microfluidic chip as claimed in claim 9.

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