System and method for image reconstruction using structure illumination microscope

By combining spatial filtering and structural illumination microscope technology, the problem of background photon overload is solved by using confocal slits and galvanometer scanning technology, and efficient super-resolution imaging and image reconstruction are achieved, and image quality and resolution are improved.

CN120500655APending Publication Date: 2025-08-15NATIONAL UNIVERSITY OF SINGAPORE
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Patent Information

Application Number
CN202380089367.2
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Priority Date
2022-12-27
Filing Date
2023-12-20
Publication Date
2025-08-15

AI Technical Summary

Technical Problem

When the imaging depth of the existing structural illumination micromirror technology increases, background photon overload leads to a decrease in image reconstruction accuracy, high noise levels, artifact amplification, and rapid image quality deterioration, making it difficult to achieve super-resolution imaging.

Method used

Combining spatial filtering and structural illumination, super-resolution images are generated by filtering fluorescence emission through confocal slits, using galvanometer scanning and rescanning techniques, combined with Fourier transform and inverse matrix processing.

Benefits of technology

Effectively reduce background photons, reduce shot noise and laser intensity noise, enhance image acquisition speed, maintain image quality and resolution at high imaging depth, and achieve super-resolution imaging.

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Abstract

In the described embodiment, a method for image reconstruction is implemented, comprising acquiring a set of original images corresponding to a plurality of phases of an illumination pattern. The image reconstruction method further includes separating the original spectrum of each original image into a baseband spectrum and at least two modulation shift spectrums using an inverse matrix, and demodulating the set of original images to generate a demodulated image. A high pass filter is applied to the baseband spectrum and a low pass filter is applied to the spectrum of the demodulated image in order to generate a corrected baseband spectrum. The corrected baseband spectrum is combined with the at least two modulation shifted spectrums to generate a synthesized spectrum. An inverse Fourier transform is performed on the synthetic spectrum to produce a reconstructed image.
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