Nutritional composition as well as preparation method, application and product thereof

Abstract

The invention belongs to the technical field of biological medicines, and particularly relates to a nutritional composition as well as a preparation method, application and a product thereof. The nutritional composition provided by the invention consists of nicotinamide, B vitamins and a fungal polysaccharide compound, the vitamin B consists of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide compound is composed of a lucid ganoderma extract, a mushroom extract, an agaricus blazei murill extract, a hericium erinaceus extract, a coprinus comatus extract, a grifola frondosa extract and a cordyceps militaris extract. Western medicine source substances (nicotinamide and B vitamins) and traditional Chinese medicine source substances (fungal polysaccharide compounds) are combined, and the nutrition composition is prepared through combination of the Chinese medicine and the western medicine. The nutritional composition has remarkable effects of enhancing immunity, delaying senescence, resisting fatigue and resisting oxidation, and shows extremely wide application prospects in the field of preparation of health-care products, medicines and cosmetics.

Description

Technical Field

[0001] The present invention belongs to the field of biomedicine technology, and specifically relates to a nutritional composition and a preparation method, application and product thereof. Background Art

[0002] Nicotinamide and the B vitamins are core players in regulating cellular metabolism, affecting both the nucleus and extranuclear organelles. Within the nucleus, they collaborate in key processes such as DNA synthesis and repair, and gene expression regulation, providing fundamental support for cellular life. Within extranuclear organelles, nicotinamide and the B vitamins also play essential roles.

[0003] In mitochondria, vitamin B1 acts as a coenzyme in the pyruvate dehydrogenase complex, driving the conversion of pyruvate to acetyl-CoA, a key step in cellular aerobic respiration. FAD and FMN, formed from vitamin B2, participate in the electron transport chain, facilitating efficient ATP synthesis. Nicotinamide's synthesis of NAD+ and NADP+ is deeply involved in mitochondrial redox reactions. NAD+ acts as an electron carrier in glycolysis and the tricarboxylic acid cycle, driving ATP production through the electron transport chain and providing a powerful driving force for cellular energy.

[0004] Ribosomes are the core site of protein synthesis. Vitamin B6 provides raw materials for protein synthesis by regulating amino acid metabolism, and may also participate in the fine regulation of ribosome function. Nicotinamide indirectly affects the efficiency and accuracy of ribosome protein synthesis by affecting the overall metabolic state of the cell, ensuring the normal renewal and function of intracellular proteins.

[0005] In the endoplasmic reticulum, vitamin B2 participates in redox reactions, maintaining redox balance within the endoplasmic reticulum and creating favorable conditions for proper protein folding and processing. It also participates in endoplasmic reticulum-related lipid metabolism, influencing the composition and function of cell membranes. Nicotinamide, by regulating intracellular signaling pathways, has a significant impact on protein processing and transport, as well as the endoplasmic reticulum's ability to store and release calcium ions, ensuring the proper functioning of intracellular substance transport and signal transduction.

[0006] Although nicotinamide and B vitamins play a wide range of roles in regulating cellular metabolism, they still have limitations when used alone. Both of them mainly focus on regulating specific metabolic pathways and functions within cells, and it is difficult to comprehensively regulate the overall cellular microenvironment and interactions between cells. For example, in terms of delaying aging, although they can maintain normal gene expression and cell division order in the cell nucleus, they have limited effects on repairing oxidative damage to the cell membrane and maintaining the extracellular matrix. In complex physiological processes such as enhancing immunity and resisting fatigue, a single ingredient is difficult to meet the comprehensive needs of the body at the multi-system and multi-cellular levels, and cannot directly regulate the activity and proliferation of immune cells, effectively relieve muscle fatigue, and restore overall energy balance.

[0007] In contrast, substances derived from traditional Chinese medicine, such as ingredients extracted from plants, animals, and fungi, can regulate the fluidity and permeability of cell membranes, optimize the cellular material exchange environment, and some are also involved in the construction of cell walls to maintain cell morphology. However, when used alone, these substances find it difficult to penetrate deep into cells and have limited effects on the core metabolic processes in the cell nucleus. For example, in terms of enhancing immunity, regulating the signal reception of immune cells only by acting on the cell membrane cannot deeply regulate the differentiation and function of immune cells at the level of gene transcription and translation; in terms of anti-fatigue, it is difficult to efficiently regulate the source of energy metabolism in the cell nucleus, and cannot fully meet the body's needs for rapid energy replenishment and metabolic repair after high-intensity activities.

[0008] Given the limitations of nicotinamide, B vitamins, and traditional Chinese medicine-derived substances in Western medicine when used alone, the combination of Chinese and Western medicine has become a mainstream trend. Patent CN117397758A discloses a Chinese and Western compound drink with immune balancing function. It uses a combination of traditional Chinese medicine ingredients and Western nutritional ingredients to balance the immune system. Patent CN118648692A provides a traditional Chinese medicine and nutrient combination with liver protection and anti-fatigue functions. It has the characteristics of combining Chinese and Western medicine. The combination has dual health care functions of liver protection and anti-fatigue, and is very suitable for people who are prone to fatigue, people who protect their livers, people with sub-health, and people who want to reduce blood sugar and lipids.

[0009] By combining the precise effects of Western medicine on key mechanisms within the cell nucleus with the comprehensive regulatory advantages of traditional Chinese medicine on cell walls, cell membranes and the overall cell microenvironment, it is hoped that comprehensive and highly effective health products can be developed. Summary of the Invention

[0010] In view of the above-mentioned deficiencies, the present invention provides a nutritional composition and its preparation method, application and product. The nutritional composition provided by the present invention is composed of nicotinamide, B vitamins and a fungal polysaccharide complex; the B vitamins are composed of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide complex is composed of Ganoderma lucidum extract, Lentinus edodes extract, Agaricus blazei extract, Hericium erinaceus extract, Coprinus comatus extract, Grifola frondosa extract and Cordyceps militaris extract. The present invention combines Western medicine-derived substances (nicotinamide, B vitamins) with Chinese medicine-derived substances (fungal polysaccharide complex), and adopts a two-pronged approach of combining Chinese and Western medicine to prepare a nutritional composition. The nutritional composition has significant effects of enhancing immunity, delaying aging, anti-fatigue and antioxidant, and has shown extremely broad application prospects in the field of health care products, medicines and cosmetics preparation.

[0011] The technical solution of the present invention is:

[0012] In a first aspect, the present invention provides a nutritional composition comprising nicotinamide, B vitamins, and a fungal polysaccharide complex;

[0013] The B vitamins are composed of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide complex is composed of ganoderma lucidum extract, shiitake mushroom extract, Agaricus blazei extract, Hericium erinaceus extract, Coprinus comatus extract, Grifola frondosa extract and Cordyceps militaris extract.

[0014] Specifically, the weight ratio of nicotinamide, B vitamins and fungal polysaccharide complex in the nutritional composition is 1-2:1-2:2-4.

[0015] Preferably, the weight ratio of nicotinamide, B vitamins and fungal polysaccharide complex in the nutritional composition is 1:1:1.

[0016] Specifically, the B vitamins include, by weight: 50-70 parts of vitamin B1, 10-30 parts of vitamin B2, 30-50 parts of vitamin B6, 0.001-0.005 parts of vitamin B12 and 0.1-0.3 parts of folic acid.

[0017] Preferably, the B vitamins include, by weight: 60 parts of vitamin B1, 20 parts of vitamin B2, 40 parts of vitamin B6, 0.001 parts of vitamin B12 and 0.2 parts of folic acid.

[0018] Specifically, the fungal polysaccharide complex comprises, by weight, 0.5-1.5 parts of Ganoderma lucidum extract, 0.5-1.5 parts of Lentinus edodes extract, 0.5-1.5 parts of Agaricus blazei extract, 0.5-1.5 parts of Hericium erinaceus extract, 0.5-1.5 parts of Coprinus comatus extract, 0.5-1.5 parts of Grifola frondosa extract and 0.25-0.75 parts of Cordyceps militaris extract.

[0019] Preferably, the fungal polysaccharide complex comprises, by weight, 1 part of Ganoderma lucidum extract, 1 part of Lentinus edodes extract, 1 part of Agaricus blazei extract, 1 part of Hericium erinaceus extract, 1 part of Coprinus comatus extract, 0.5 part of Grifola frondosa extract and 0.5 part of Cordyceps militaris extract.

[0020] In a second aspect, the present invention provides a method for preparing the above-mentioned nutritional composition, which comprises uniformly mixing nicotinamide, B vitamins and a fungal polysaccharide complex.

[0021] Specifically, the preparation method of the B vitamins includes: uniformly mixing vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid.

[0022] Specifically, the preparation method of the fungal polysaccharide complex comprises: uniformly mixing Ganoderma lucidum extract, Lentinus edodes extract, Agaricus blazei extract, Hericium erinaceus extract, Coprinus comatus extract, Grifola frondosa extract and Cordyceps militaris extract.

[0023] In a third aspect, the present invention provides the use of the above-mentioned nutritional composition or the nutritional composition obtained by the above-mentioned preparation method in the preparation of products for enhancing immunity, resisting fatigue or resisting aging.

[0024] In a fourth aspect, the present invention provides a product, comprising the above-mentioned nutritional composition or the nutritional composition obtained by the above-mentioned preparation method.

[0025] Specifically, the nutritional composition in the product is the only or main active ingredient of the product.

[0026] Specifically, the nutritional composition in the product is used in combination with other active ingredients.

[0027] Preferably, the other active ingredients include one or more of fungal polysaccharides, animal polysaccharides, plant polysaccharides, algal polysaccharides, vitamin B3 and derivatives thereof.

[0028] Further preferably, the fungal polysaccharide includes but is not limited to one or more of: Poria cocos polysaccharide, Tremella fuciformis polysaccharide, Flammulina velutipes polysaccharide, Auricularia auricularia polysaccharide, Cordyceps sinensis polysaccharide, Phellinus igniarius polysaccharide, and Coriolus versicolor polysaccharide.

[0029] Further preferably, the animal polysaccharide includes but is not limited to one or more of glycogen, chondroitin sulfate, hyaluronic acid, heparin, chitosan, chitin, and glycosaminoglycan.

[0030] Further preferably, the plant polysaccharides include but are not limited to one or more of starch, cellulose, hemicellulose, pectin, inulin, konjac polysaccharide, astragalus polysaccharide, wolfberry polysaccharide, tea polysaccharide, kelp polysaccharide, and jujube polysaccharide.

[0031] Further preferably, the algae polysaccharide includes but is not limited to: one or more of green algae polysaccharide, red algae polysaccharide, and brown algae polysaccharide.

[0032] Further preferably, the vitamin B3 and its derivatives include but are not limited to: one or more of niacin, nicotinamide riboside, β-nicotinamide mononucleotide, NAD+, and NADH.

[0033] Specifically, the products include medicines for enhancing immunity, resisting fatigue, resisting oxidation or resisting aging.

[0034] Preferably, the drug further comprises one or more pharmaceutically acceptable excipients.

[0035] Further preferably, the pharmaceutically acceptable excipients include but are not limited to: solvents, diluents, disintegrants, precipitation inhibitors, surfactants, glidants, adhesives, lubricants, dispersants, suspending agents, isotonic agents, thickeners, emulsifiers, preservatives, stabilizers, hydrating agents, emulsification accelerators, buffers, absorbents, colorants, flavoring agents, sweeteners, ion exchangers, release agents, coating agents, flavoring agents or antioxidants.

[0036] Preferably, the dosage form of the drug includes but is not limited to tablets, pills, powders, suspensions, gels, emulsions, creams, granules, capsules, suppositories, injections, sprays or injections.

[0037] Specifically, the products include health products for enhancing immunity, resisting fatigue or resisting oxidation.

[0038] Preferably, the health care product further comprises nutritional additives that are nutritionally acceptable.

[0039] Further preferably, the nutritional additives include but are not limited to: one or more of dietary fiber, prebiotics, protein, lipids, minerals, and vitamins.

[0040] Further preferably, the dosage form of the health care product includes but is not limited to: tablets, capsules, soft capsules, granules, pills, gel candies, powders, oral liquids or drops.

[0041] Specifically, the products include cosmetics for anti-aging or anti-oxidation.

[0042] Preferably, the cosmetics further include auxiliary materials acceptable in the cosmetics field.

[0043] Further preferably, the cosmetics acceptable excipients include but are not limited to: one or more of moisturizers, thickeners, emollients, skin conditioners, emulsifiers, preservatives, emollients, lubricants, and stabilizers.

[0044] Further preferably, the cosmetics include face cream, facial mask, eye cream, neck cream, hand cream, body lotion, moisturizing lotion, essence, toner, shampoo, conditioner, shower gel or cleanser.

[0045] The beneficial effects of the present invention are:

[0046] The present invention combines Western medicinal substances (niacinamide and B vitamins) with traditional Chinese medicinal substances (fungal polysaccharide complexes), creating a nutritional composition that combines both Western and Chinese medicinal properties. This nutritional composition exhibits significant immune-boosting, anti-aging, anti-fatigue, and antioxidant benefits. Therefore, it has broad application prospects in the preparation of health products, pharmaceuticals, and cosmetics. BRIEF DESCRIPTION OF THE DRAWINGS

[0047] Figure 1 Figure 2 is a sample of MTC zebrafish phenotypes (n=30).

[0048] Figure 2 The fluorescence phenotype of zebrafish neutrophils (n=30).

[0049] Figure 3 The mean fluorescence intensity of zebrafish neutrophils (n=30); **** in the figure represents P<0.0001.

[0050] Figure 4 The fluorescence phenotype of zebrafish macrophages (n=30).

[0051] Figure 5 The mean fluorescence intensity of zebrafish macrophages (n=30); **** in the figure represents P<0.0001.

[0052] Figure 6 The ROS fluorescence staining phenotype of zebrafish (n=30).

[0053] Figure 7 The mean fluorescence intensity of ROS staining in zebrafish (n=30); **** in the figure represents P<0.0001.

[0054] Figure 8 Telomerase activity in zebrafish (n=30); **** in the figure represents P<0.0001.

[0055] Figure 9 is the zebrafish β-galactosidase activity (n=30); **** in the figure represents P<0.0001.

[0056] Figure 10 The figure shows the trajectory of zebrafish movement behavior (n=30).

[0057] Figure 11 is the total movement distance of zebrafish (n=30).

[0058] Figure 12 is the average movement speed of zebrafish (n=30).

[0059] Figure 13 is the ATP content of zebrafish; *** in the figure represents P < 0.001. DETAILED DESCRIPTION

[0060] The present invention will be further clarified and fully described below by way of examples. The following examples are only a portion of the present invention and are not intended to limit the present invention, but are merely for illustration. The experimental methods used in the following examples are all routine experiments unless otherwise specified, and the materials and reagents used in the following examples are all commercially available unless otherwise specified.

[0061] The raw material information used in the present invention is as follows:

[0062] β-Nicotinamide mononucleotide (NMN) was purchased from Shandong Yuanlitai Pharmaceutical Technology Co., Ltd.; nicotinamide was purchased from Jiangsu Caiwei Biotechnology Co., Ltd.; vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid were all purchased from Huazhong Pharmaceutical Co., Ltd.; Ganoderma lucidum extract, shiitake mushroom extract and maitake mushroom extract were all purchased from Shaanxi Huaze Biotechnology Co., Ltd.; Agaricus blazei Murrill extract, Hericium erinaceus extract, Coprinus comatus extract and Cordyceps militaris extract were all purchased from Hangzhou Zhongzhi Kanggu Biotechnology Co., Ltd.

[0063] The information of the experimental animals used in this invention is as follows:

[0064] Wild-type AB strains, neutrophil fluorescent transgenic Tg (mpx:GFP), and macrophage fluorescent transgenic zebrafish Tg (mpeg1:EGFP) were all provided by Feifan's standard zebrafish drug screening platform. The test fish were zebra danio (Teleosteiformes, Cyprinidae) (Danio rerio). The test fish were 30±5 mm in length and 0.3±0.1 g in weight, selected from juveniles of uniform size from the same acclimation pond. Prior to the test, the fish were acclimated for at least two weeks in continuously aerated water under the same environmental conditions used during the experiment. Feeding was stopped 24 hours prior to the test, and feces and food debris were removed daily. The mortality rate during the acclimation period did not exceed 10%. If this rate exceeded 10%, the batch of fish was not used for testing. The test fish showed no obvious disease or macroscopic deformities. No disease treatment was administered within two weeks prior to the test.

[0065] The data processing method of the present invention:

[0066] SPSS26 software was used for analysis, and GraphPad Prism 8 was used for drawing. The data statistics were expressed as mean ± standard deviation. The experimental results were tested for normal distribution using SPSS software. If the data were in accordance with the normal distribution, the T parametric test or analysis of variance (ANOVA) was used for significance. If the data were not in accordance with the normal distribution, the nonparametric test was used (the result P value < 0.05 indicated statistically significant difference).

[0067] Example 1 Preparation of experimental samples

[0068] B vitamins:

[0069] According to parts by weight, 60 parts of vitamin B1, 20 parts of vitamin B2, 40 parts of vitamin B6, 0.001 parts of vitamin B12 and 0.2 parts of folic acid are uniformly mixed to prepare B vitamins.

[0070] Fungal polysaccharide complex:

[0071] According to weight proportions, evenly mix 1 part of Ganoderma lucidum extract, 1 part of Lentinus edodes extract, 1 part of Agaricus blazei extract, 1 part of Hericium erinaceus extract, 1 part of Coprinus comatus extract, 0.5 part of Grifola frondosa extract and 0.5 part of Cordyceps militaris extract.

[0072] Composition 1:

[0073] Nicotinamide and B vitamins were mixed in a weight ratio of 1:1 to obtain composition 1.

[0074] Composition 2:

[0075] Nicotinamide and the fungal polysaccharide complex were mixed in a weight ratio of 1:1 to obtain composition 2.

[0076] Composition 3:

[0077] The B vitamins and the fungal polysaccharide complex were mixed in a weight ratio of 1:1 to obtain composition 3.

[0078] Nutritional composition:

[0079] Nicotinamide, B vitamins and fungal polysaccharide complex are mixed in a weight ratio of 1:1:1 to obtain a nutritional composition.

[0080] Example 2: Exploration of MTC in zebrafish

[0081] 1. Experimental groups

[0082] This example is divided into five groups: a normal control group, an NMN group, a nicotinamide group, a B vitamin group, and a fungal polysaccharide complex group. The dissolution medium, zebrafish culture water, is used as the normal control group. The MTC of the four samples (NMN, nicotinamide, B vitamins, and fungal polysaccharide complex) is verified. Three detection concentrations are used for each sample, and 30 zebrafish are used in each group.

[0083] 2. Experimental methods

[0084] After sample processing, the number of zebrafish deaths and toxicity in each experimental group were counted to determine the sample's MTC. If the MTC cannot be obtained in the initial concentration testing experiment, the sample testing concentration range will be expanded to an upper limit of 2000 μg / mL or maximum solubility and a lower limit of 0.01 μg / mL. Evaluation Metrics: The number of zebrafish deaths and toxicity in each experimental group were counted to determine the sample's MTC. Results: Three representative zebrafish images for each sample and concentration, along with a mortality statistics table.

[0085] 3. Experimental results

[0086] Under the conditions of this example, each sample had no obvious toxic side effects on the model zebrafish at a concentration of 125-500 μg / mL; the MTC of each sample was 500 μg / mL. The results are shown in Table 1 and Figure 1 shown.

[0087] Table 1 Sample concentration exploratory test results (n=30)

[0088]

[0089] Example 3 Determination of the Immunity-Enhancing Effect

[0090] 1. Experimental groups

[0091] This example is divided into 10 groups, namely blank control group, model control group, positive control group (NMN group), nicotinamide group, B vitamin group, fungal polysaccharide complex group, composition group 1, composition group 2, composition group 3 and nutritional composition group, with 30 fish in each group.

[0092] 2. Experimental methods

[0093] Except for the blank control group, 10 nL of 200 mg / mL LPS solution was injected into the yolk sac of each juvenile fish in the remaining groups to induce an immune response in the zebrafish. The samples were then processed as follows:

[0094] Blank control group: no special treatment.

[0095] Model control group: no special treatment.

[0096] Positive control group: NMN was administered at a final concentration of 500 μg / mL.

[0097] Nicotinamide group: Nicotinamide was administered at a final concentration of 500 μg / mL.

[0098] Vitamin B group: administered with a final concentration of 500 μg / mL of vitamin B.

[0099] Fungal polysaccharide complex group: a fungal polysaccharide complex with a final concentration of 500 μg / mL was administered.

[0100] Composition 1 group: Composition 1 was administered at a final concentration of 500 μg / mL.

[0101] Composition 2 group: Composition 2 was administered at a final concentration of 500 μg / mL.

[0102] Composition 3 group: Composition 3 was administered at a final concentration of 500 μg / mL.

[0103] Nutritional composition group: administered with a nutritional composition having a final concentration of 500 μg / mL.

[0104] After 24 hours of sample treatment, the zebrafish were placed under a fluorescence microscope and photographed, and the number of neutrophils and macrophages in the zebrafish was measured and analyzed using Image J image processing software.

[0105] 3. Experimental results

[0106] The results of zebrafish neutrophil fluorescence phenotype and quantitative analysis are as follows Figure 2-Figure 3 As shown in Figure 2, the fluorescence phenotype and quantitative analysis of zebrafish macrophages are shown in Figure 2. Figure 4-Figure 5 shown.

[0107] The results showed that the number of neutrophils and macrophages stained in zebrafish induced by LPS was statistically significantly increased, as shown by an increase in the mean fluorescence intensity, which means that the model was successfully established. Compared with the model control group, nicotinamide, B vitamins, and fungal polysaccharide complexes administered alone or in combination to zebrafish showed varying degrees of reduction in the number of neutrophils and macrophages and weakened fluorescence intensity, which means an improvement in the immune response. Among them, the nutritional composition group, which mixed nicotinamide, B vitamins, and fungal polysaccharide complexes, showed the best immunity-enhancing ability, which was better than the positive control group.

[0108] Determination of the anti-aging effect of Example 4

[0109] 1. Experimental groups

[0110] This example is divided into 10 groups, namely blank control group, model control group, positive control group (NMN group), nicotinamide group, B vitamin group, fungal polysaccharide complex group, composition group 1, composition group 2, composition group 3 and nutritional composition group, with 30 fish in each group.

[0111] 2. Experimental methods

[0112] Except for the blank control group, the remaining groups used H2O2 at a final concentration of 200 μM to establish a zebrafish oxidative stress model, and then processed the samples. The processing methods for each group are as follows:

[0113] Blank control group was added: no special treatment was performed.

[0114] Model control group added: no special treatment.

[0115] Positive control group: NMN was administered at a final concentration of 500 μg / mL.

[0116] Nicotinamide group: Nicotinamide was administered at a final concentration of 500 μg / mL.

[0117] Vitamin B group: administered with a final concentration of 500 μg / mL of vitamin B.

[0118] Fungal polysaccharide complex group: a fungal polysaccharide complex with a final concentration of 500 μg / mL was administered.

[0119] Composition 1 group: Composition 1 was administered at a final concentration of 500 μg / mL.

[0120] Composition 2 group: Composition 2 was administered at a final concentration of 500 μg / mL.

[0121] Composition 3 group: Composition 3 was administered at a final concentration of 500 μg / mL.

[0122] Nutritional composition group: administered with a nutritional composition having a final concentration of 500 μg / mL.

[0123] After 24 hours of sample treatment, zebrafish were incubated with a ROS staining kit, washed, and photographed under a fluorescence microscope. Image J image processing software was used to measure and analyze the ROS staining intensity in the zebrafish. An Elisa kit was used to quantify the telomerase concentration and β-galactosidase activity in the zebrafish according to the standard curve.

[0124] 3. Experimental results

[0125] Zebrafish ROS fluorescence phenotype and quantitative analysis Figure 6-Figure 7 The quantitative results of zebrafish telomerase activity are shown in Figure 8 , the results of zebrafish β-galactosidase activity are shown in Figure 9 .

[0126] By adding HO to the culture medium of zebrafish larvae for 24-48 hours, oxidative damage was induced in zebrafish and an acute aging model was prepared. The experimental results showed that compared with the model group, nicotinamide, B vitamins, and fungal polysaccharide complexes, when applied to zebrafish alone or in combination, showed: (1) a decrease in ROS fluorescence intensity, and the stronger the ROS fluorescence intensity, the deeper the degree of oxidative stress, indicating that each group showed good antioxidant function; (2) an increase in telomerase concentration, indicating that each group showed good anti-aging potential and delayed the aging process; (3) a decrease in β-galactosidase activity, which means that the degree of aging was weakened, and anti-aging substances should reduce its activity. Among them, the nutritional composition group mixed nicotinamide, B vitamins, and fungal polysaccharide complexes, achieving the best technical effect.

[0127] Example 5 Anti-fatigue function evaluation

[0128] 1. Experimental groups

[0129] This example is divided into 10 groups, namely blank control group, model control group, positive control group (NMN group), nicotinamide group, B vitamin group, fungal polysaccharide complex group, composition group 1, composition group 2, composition group 3 and nutritional composition group, with 30 fish in each group.

[0130] 2. Experimental methods

[0131] Except for the blank control group, the rest of the groups used sodium sulfite at a final concentration of 1000 μM to establish a zebrafish fatigue model, and then processed the samples. The processing methods for each group are as follows:

[0132] Blank control group: no special treatment.

[0133] Model control group: no special treatment.

[0134] Positive control group: NMN was administered at a final concentration of 500 μg / mL.

[0135] Nicotinamide group: Nicotinamide was administered at a final concentration of 500 μg / mL.

[0136] Vitamin B group: administered with a final concentration of 500 μg / mL of vitamin B.

[0137] Fungal polysaccharide complex group: a fungal polysaccharide complex with a final concentration of 500 μg / mL was administered.

[0138] Composition 1 group: Composition 1 was administered at a final concentration of 500 μg / mL.

[0139] Composition 2 group: Composition 2 was administered at a final concentration of 500 μg / mL.

[0140] Composition 3 group: Composition 3 was administered at a final concentration of 500 μg / mL.

[0141] Nutritional composition group: administered with a nutritional composition having a final concentration of 500 μg / mL.

[0142] After 48 hours of sample treatment, the ATP content in the zebrafish was quantified using an ATP assay kit according to the standard curve. The movement behavior of the zebrafish within 10 minutes was recorded using Daniovision, and the total movement distance and movement rate were quantified.

[0143] 3. Experimental results

[0144] The anti-fatigue effects of nicotinamide, B vitamins, and fungal polysaccharide complexes alone or in combination on zebrafish were evaluated. Figure 10-12 The quantitative results of zebrafish ATP activity are shown in Figure 13 As shown. Compared with the control group, nicotinamide, B vitamins, and fungal polysaccharide complexes all effectively improved the athletic ability of zebrafish. In particular, the combination of these three substances in a nutritional composition showed the most significant improvements in zebrafish ATP content, movement distance, and speed, indicating its potential application value in fatigue resistance. Increased ATP levels help restore and maintain cellular energy metabolism. The nutritional composition of the present invention can effectively enhance the exercise endurance of zebrafish, improve athletic performance, and reduce fatigue.

[0145] The above detailed description is a specific description of one feasible embodiment of the present invention and is not intended to limit the scope of the present invention. It should be noted that any equivalent implementation or modification that does not depart from the present invention should be included within the scope of the technical solution of the present invention. Therefore, the scope of protection of the patent of this invention should be based on the attached requirements.

Claims

1. A nutritional composition, characterized in that The nutritional composition consists of nicotinamide, B vitamins and fungal polysaccharide complex; The B vitamins are composed of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide complex is composed of ganoderma lucidum extract, shiitake mushroom extract, Agaricus blazei extract, Hericium erinaceus extract, Coprinus comatus extract, Grifola frondosa extract and Cordyceps militaris extract.

2. The nutritional composition according to claim 1, characterized in that The weight ratio of nicotinamide, B vitamins and fungal polysaccharide complex in the nutritional composition is 1-2:1-2:2-4; Preferably, the weight ratio of nicotinamide, B vitamins and fungal polysaccharide complex in the nutritional composition is 1:1:

1.

3. The nutritional composition according to claim 1, characterized in that The B vitamins include, by weight: 50-70 parts of vitamin B1, 10-30 parts of vitamin B2, 30-50 parts of vitamin B6, 0.001-0.005 parts of vitamin B12 and 0.1-0.3 parts of folic acid; Preferably, the B vitamins include, by weight: 60 parts of vitamin B1, 20 parts of vitamin B2, 40 parts of vitamin B6, 0.001 parts of vitamin B12 and 0.2 parts of folic acid.

4. The nutritional composition according to claim 1, characterized in that The fungal polysaccharide complex comprises, by weight, 0.5-1.5 parts of Ganoderma lucidum extract, 0.5-1.5 parts of Lentinus edodes extract, 0.5-1.5 parts of Agaricus blazei extract, 0.5-1.5 parts of Hericium erinaceus extract, 0.5-1.5 parts of Coprinus comatus extract, 0.5-1.5 parts of Grifola frondosa extract, and 0.25-0.75 parts of Cordyceps militaris extract. Preferably, the fungal polysaccharide complex comprises, by weight, 1 part of Ganoderma lucidum extract, 1 part of Lentinus edodes extract, 1 part of Agaricus blazei extract, 1 part of Hericium erinaceus extract, 1 part of Coprinus comatus extract, 0.5 part of Grifola frondosa extract and 0.5 part of Cordyceps militaris extract.

5. The method for preparing the nutritional composition according to any one of claims 1 to 4, characterized in that: The preparation method comprises uniformly mixing nicotinamide, B vitamins and fungal polysaccharide complex; The preparation method of the B vitamins comprises: uniformly mixing vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; The preparation method of the fungal polysaccharide complex comprises: uniformly mixing ganoderma lucidum extract, shiitake mushroom extract, Agaricus blazei extract, Hericium erinaceus extract, Coprinus comatus extract, Grifola frondosa extract and Cordyceps militaris extract.

6. Use of the nutritional composition according to any one of claims 1 to 4 or the nutritional composition obtained by the preparation method according to claim 5 in preparing products for enhancing immunity, resisting fatigue or resisting aging.

7. A product, characterized in that The product comprises the nutritional composition according to any one of claims 1 to 4 or the nutritional composition obtained by the preparation method according to claim 5.

8. The product according to claim 7, characterized in that The nutritional composition in the product is the only or main active ingredient of the product.

9. The product according to claim 7, characterized in that The nutritional composition in the product is used in combination with other active ingredients.

10. The product according to claim 9, characterized in that The other active ingredients include one or more of fungal polysaccharides, animal polysaccharides, plant polysaccharides, algae polysaccharides, vitamin B3 and derivatives thereof.

11. The product according to claim 10, characterized in that The fungal polysaccharide includes one or more of: Poria cocos polysaccharide, Tremella fuciformis polysaccharide, Flammulina velutipes polysaccharide, Auricularia auricularia polysaccharide, Cordyceps sinensis polysaccharide, Phellinus igniarius polysaccharide, and Coriolus versicolor polysaccharide; Or the animal polysaccharide includes one or more of glycogen, chondroitin sulfate, hyaluronic acid, heparin, chitosan, chitin, and glycosaminoglycan; Or the plant polysaccharide includes one or more of starch, cellulose, hemicellulose, pectin, inulin, konjac polysaccharide, astragalus polysaccharide, wolfberry polysaccharide, tea polysaccharide, kelp polysaccharide, and red date polysaccharide; Or the algae polysaccharide includes one or more of green algae polysaccharide, red algae polysaccharide, and brown algae polysaccharide; Or the vitamin B3 and its derivatives include: one or more of niacin, nicotinamide riboside, β-nicotinamide mononucleotide, NAD+, and NADH.

12. The product according to claim 7, characterized in that The products include medicines for enhancing immunity, resisting fatigue, resisting oxidation or resisting aging.

13. The product according to claim 12, characterized in that The medicine also includes one or more pharmaceutically acceptable excipients.

14. The product according to claim 12, characterized in that The dosage forms of the drugs include tablets, pills, powders, suspensions, gels, emulsions, creams, granules, capsules, suppositories, injections, sprays or injections.

15. The product according to claim 7, characterized in that The products include health products for enhancing immunity, resisting fatigue or resisting oxidation.

16. The product according to claim 15, characterized in that The health care product also includes nutritional additives that are nutritionally acceptable.

17. The product according to claim 7, characterized in that The products include cosmetics for anti-aging or anti-oxidation.

18. The product according to claim 17, characterized in that The cosmetics also include auxiliary materials acceptable in the cosmetics field.

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