Traditional Chinese medicine preparation for treating waterfowl reovirus and application of traditional Chinese medicine preparation

Abstract

The invention relates to the technical field of veterinary medicine, and discloses a traditional Chinese medicine preparation for treating waterfowl reovirus, the medicine preparation is prepared from the following raw materials in parts by weight: 20-40 parts of astragalus membranaceus, 20-40 parts of scutellaria baicalensis, 10-20 parts of liquorice, 15-30 parts of platycodon grandiflorum, 20-30 parts of herba epimedii, 10-30 parts of honeysuckle, 10-30 parts of wild chrysanthemum flower, 20-30 parts of semen coicis and 20-35 parts of lalang grass rhizome, the immunity of waterfowls is enhanced; the radix scutellariae has the effects of clearing heat, drying dampness, purging fire, removing toxin and clearing away heat toxin in waterfowl bodies; the liquorice harmonizes the medicines and clears away heat and toxic materials; radix platycodonis freezes the lung, relieves sore throat, expels pus and Herba epimedii can warm kidney and strengthen yang, dispel wind and eliminate dampness, and regulate physiological functions of waterfowls; honeysuckle and wild chrysanthemum flower clear away heat and toxic materials, cool blood and subdue swelling Semen coicis can clear damp and promote diuresis, and tonify spleen and The lalang grass rhizome has the effects of reducing fever, causing diuresis, cooling blood and stopping bleeding, and the traditional Chinese medicine components are matched with one another to jointly treat waterfowl reovirus.

Description

Technical Field

[0001] The present invention relates to the technical field of veterinary medicine, in particular to a traditional Chinese medicine preparation for treating waterfowl reocolitis and application thereof. Background Art

[0002] Reocongestive heart disease (RCD) is a serious health hazard for waterfowl, with high morbidity and mortality, resulting in significant economic losses for the waterfowl farming industry. Reocongestive heart disease is primarily prevented through immunization, and treatments for waterfowl RCD are primarily chemical agents. Long-term use can lead to drug resistance and residual drug residues, negatively impacting the quality of waterfowl products and the ecological environment. Therefore, developing a safe, effective, and residue-free traditional Chinese medicine (TCM) preparation for the treatment of RCD is of great significance. Summary of the Invention

[0003] In view of the deficiencies in the prior art, the present invention provides a traditional Chinese medicine preparation for treating waterfowl reocolitis and its application.

[0004] To achieve the above-mentioned purpose, the present invention provides the following technical solution: a traditional Chinese medicine preparation for treating waterfowl renal intestinal disease, the medicine preparation being composed of the following raw materials in parts by weight: 20-40 parts of astragalus, 20-40 parts of scutellaria, 10-20 parts of licorice, 15-30 parts of platycodon, 20-30 parts of epimedium, 10-30 parts of honeysuckle, 10-30 parts of wild chrysanthemum, 20-30 parts of coix seed, and 20-35 parts of Imperata rhizome.

[0005] Preferably, Astragalus membranaceus replenishes Qi and strengthens the exterior, promotes diuresis and reduces swelling, and enhances the immunity of waterfowl; Scutellaria baicalensis clears away heat and dampness, purges fire and detoxifies, and removes heat and toxins from the bodies of waterfowl; Licorice harmonizes various medicines, clears away heat and detoxifies; Platycodon grandiflorum promotes lung function and relieves sore throat, discharges pus and detoxifies; Epimedium warms the kidney and strengthens yang, dispels wind and dampness, and regulates the physiological functions of waterfowl; Honeysuckle and Wild Chrysanthemum clear away heat and detoxify, cools blood and reduces swelling; Coix seed promotes diuresis and eliminates dampness, strengthens the spleen and eliminates numbness; Imperata rhizome clears heat and promotes diuresis, cools blood and stops bleeding. These Chinese medicinal ingredients cooperate with each other to jointly treat respiratory soreness of waterfowl.

[0006] Preferably, the preparation method thereof is as follows: Step 1: treating the scutellaria baicalensis with enzyme: drying the scutellaria baicalensis at 60-80° C. for 2-4 hours, and crushing the scutellaria baicalensis in the formula amount to a particle size of less than 3 mm; Mix the crushed Scutellaria baicalensis with water in a weight ratio of 1:15-20, stir evenly, and adjust the pH value to 4.5-6.0; Add 150-250 U of cellulase per gram of Scutellaria baicalensis, add the cellulase to the above mixture, and perform enzymolysis at a temperature of 45-55°C for 3-5 hours; After the enzymatic hydrolysis is completed, the mixed solution is heated to 80-90°C and maintained for 10-15 minutes to terminate the enzyme reaction, and then filtered to obtain the enzyme-treated liquid.

[0007] Preferably, the enzymatically hydrolyzed drug solution is centrifuged at a speed of 3000-5000 rpm for 10-15 minutes to remove impurity particles and improve the clarity of the preparation.

[0008] Preferably, step 2: stir-frying coix seed until the surface turns slightly yellow, weighing astragalus, licorice, platycodon, epimedium, honeysuckle, wild chrysanthemum, coix seed and imperata rhizome according to the above ratio, adding appropriate amount of water, and soaking for 0.5 hour; Add water and decoct twice. The first time, add 8-10 times the total weight of the medicinal materials and decoct for 1-2 hours. The second time, add 6-8 times the total weight of the medicinal materials and decoct for 0.5-1 hour. Combine the two decoctions, filter, add the enzyme-treated solution in step 1, and concentrate to an extract with a relative density of 1.10-1.25 (60°C).

[0009] Preferably, the preparation method further comprises an extract purification step: adding 3-5 times the volume of 95% ethanol to the concentrated extract, standing for 24 hours, filtering and recovering the ethanol, so as to increase the purity of the active ingredient by 10-15%.

[0010] Preferably, step three: spray-drying the extract to make powder, or adding appropriate amount of auxiliary materials (starch, dextrin) to make granules.

[0011] Preferably, the extract is dissolved in purified water, 0.1-0.5% sodium benzoate is added as a preservative, the pH value is adjusted to 6.0-8.0, filtered through a 0.45 μm filter membrane, and then filled to remove impurities and improve the clarity of the preparation, and sterilized by moist heat (115°C, 30 minutes) to prepare an oral dosage form; Preferably, after the preparation is made, geese suffering from reocolitis are selected as experimental subjects and randomly divided into two groups; the Chinese medicine preparation of the present invention is used for treatment, with the drug administered twice a day for 7 consecutive days; After the treatment, the disease condition changes, cure rates, and efficacy rates of the two groups of geese were observed.

[0012] Preferably, the use of a traditional Chinese medicine preparation for treating waterfowl reocongestive disease, the preparation is used to treat waterfowl reocongestive disease, relieve the symptoms of waterfowl reocongestive disease, and improve the cure rate of waterfowl.

[0013] Compared with the prior art, the present invention provides a traditional Chinese medicine preparation for treating waterfowl reocolitis and its application, which has the following beneficial effects: 1. The traditional Chinese medicine preparation for treating waterfowl reoenteroidal disease and its application. The traditional Chinese medicine preparation uses a variety of natural Chinese medicines as raw materials, applies the principle of traditional Chinese medicine differentiation and treatment to rationally combine and uses special enzyme treatment and extraction and concentration technology. It has good therapeutic effect and safety, can effectively alleviate the symptoms of waterfowl reoenteroidal disease, and improve the cure rate and survival rate of waterfowl.

[0014] 2. The traditional Chinese medicine preparation for treating waterfowl reoenteropathia and its application have significant therapeutic effects: through the scientific combination of multiple traditional Chinese medicine ingredients, comprehensive treatment is carried out on the causes and symptoms of waterfowl reoenteropathia, effectively relieving the symptoms of waterfowl such as fever, mental depression, loss of appetite, diarrhea, etc., and improving the cure rate.

[0015] 3. The traditional Chinese medicine preparation for treating waterfowl reoenteroidal disease and its application are derived from natural plants, have no chemical drug residues, and do not pollute the quality of waterfowl products and the ecological environment. At the same time, no obvious adverse reactions were found in the experiment, and the use is safe and reliable.

[0016] 4. The traditional Chinese medicine preparation for treating waterfowl reocongestive disease and its application are not only suitable for treating goose reocongestive disease, but can also be used to treat duck and other waterfowl reocongestive disease, and have wide applicability.

[0017] 5. The traditional Chinese medicine preparation for treating waterfowl reoenteroidal disease and its application have advanced technology: enzyme treatment technology is used to pre-treat some medicinal materials, which improves the extraction rate and efficacy of effective ingredients, increases the effect of the prescription, and makes the quality of the traditional Chinese medicine preparation more stable and reliable.

[0018] 6. The traditional Chinese medicine preparation for treating waterfowl reoenteroidal disease and its application, the medicine preparation is prepared into powder, granules, and oral dosage forms in three ways, which can be adapted to multiple feeding scenarios such as group feeding scenarios and individual feeding scenarios, so as to better use the medicine preparation. DETAILED DESCRIPTION

[0019] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the embodiments described are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0020] A traditional Chinese medicine preparation for treating waterfowl regurgitation disease and its application, the preparation is composed of the following raw materials in parts by weight: 20-40 parts of astragalus, 20-40 parts of scutellaria, 10-20 parts of licorice, 15-30 parts of platycodon, 20-30 parts of epimedium, 10-30 parts of honeysuckle, 10-30 parts of wild chrysanthemum, 20-30 parts of coix seed, and 20-35 parts of imperata rhizome.

[0021] In Example 1, the Chinese medicinal components in parts by weight are: 25-35 parts of astragalus, 25-35 parts of scutellaria, 12-18 parts of licorice, 15-25 parts of platycodon, 20-28 parts of epimedium, 15-28 parts of honeysuckle, 10-20 parts of wild chrysanthemum, 23-30 parts of coix seed, and 20-30 parts of imperata rhizome.

[0022] In Example 2, the Chinese medicinal components in parts by weight are: 30 parts of Astragalus, 28 parts of Scutellaria, 15 parts of Licorice, 20 parts of Platycodon, 25 parts of Epimedium, 20 parts of Honeysuckle, 15 parts of Chrysanthemum indicum, 25 parts of Coix seeds, and 25 parts of Imperata rhizome.

[0023] Example 3, the Chinese medicinal components in parts by weight are: 30 parts of Astragalus, 28 parts of Scutellaria, 15 parts of Licorice, 20 parts of Platycodon, 25 parts of Epimedium, 20 parts of Honeysuckle, 15 parts of Chrysanthemum indicum, 25 parts of Coix seeds, 25 parts of Imperata rhizome, and also include 10-20 parts of Isatis root or 10-15 parts of Forsythia.

[0024] Among them, Astragalus membranaceus has the effects of tonifying Qi, strengthening the exterior, promoting diuresis and reducing swelling, and can enhance the immunity of waterfowl; Scutellaria baicalensis clears heat and dampness, purging fire and detoxifying, and effectively eliminates heat and toxins in waterfowl; Licorice root harmonizes the various herbs, clears heat and detoxifies; Platycodon grandiflorum clears the lungs and relieves sore throat, drains pus and detoxifies; Epimedium warms the kidneys and strengthens yang, dispels wind and dampness, and helps regulate the physiological functions of waterfowl; Honeysuckle and Wild Chrysanthemum clear heat and detoxify, cool the blood and reduce swelling; Coix seed promotes diuresis and eliminates dampness, strengthens the spleen and eliminates numbness; Imperata root clears heat and promotes diuresis, cools the blood and stops bleeding. These Chinese medicinal ingredients work together to treat regurgitation in waterfowl.

[0025] Enzyme treatment of scutellaria baicalensis: the scutellaria baicalensis is dried at 60-80°C for 2-4 hours, and the formulated amount of scutellaria baicalensis is crushed to a particle size of less than 3 mm; the crushed scutellaria baicalensis is mixed with water at a weight ratio of 1:15-20, stirred evenly, and the pH value is adjusted to 4.5-6.0; 150-250U of cellulase is added to the above mixture per gram of scutellaria baicalensis, and the cellulase is added to the above mixture, and enzymatic hydrolysis is carried out at a temperature of 45-55°C for 3-5 hours; after the enzymatic hydrolysis is completed, the mixture is heated to 80-90°C and maintained for 10-15 minutes to terminate the enzyme reaction, and filtered to obtain an enzyme-treated medicinal solution.

[0026] Stir-fry coix seed until the surface turns slightly yellow, weigh astragalus, licorice, platycodon, epimedium, honeysuckle, wild chrysanthemum, coix seed, imperata root, indigo root or forsythia according to the above ratio, add appropriate amount of water, and soak for 0.5 hour; add water and boil twice, the first time adding water amount is 8-10 times the total weight of the medicinal materials, boiling for 1-2 hours, the second time adding water amount is 6-8 times the total weight of the medicinal materials, boiling for 0.5-1 hour, combine the two decoctions, filter, add the enzyme-treated liquid in step 1, and concentrate to an extract with a relative density of 1.10-1.25 (60°C).

[0027] The preparation method also includes an extract purification step: adding 3-5 times the volume of 95% ethanol to the concentrated extract, letting it stand for 24 hours, filtering and recovering the ethanol, so that the purity of the active ingredient is increased by 10-15%.

[0028] The extract is spray-dried to make powder, or added with appropriate amount of auxiliary materials (starch, dextrin) to make granules.

[0029] The extract was dissolved in purified water, 0.1-0.5% sodium benzoate was added as a preservative, the pH value was adjusted to 6.0-8.0, and the extract was filtered through a 0.45 μm filter membrane before filling to remove impurities and improve the clarity of the preparation. The extract was sterilized by moist heat (115°C, 30 minutes) to prepare an oral dosage form. In order to verify the efficacy of the Chinese medicine preparation of the present invention, the following experiments were performed: Example 4 At a farm in Jixian County, Heilongjiang Province, breeding 20,000 Pu San meat geese, symptoms of lameness, lethargy, and decreased feed intake were noted in 15-day-old geese. Three days later, the number of affected geese had grown to over 2,000. The geese were emaciated, with disheveled feathers, depressed, and lame. Some geese even passed loose yellow-green stools and limped. Autopsies revealed intestinal bleeding, enlarged livers with grayish-white necrotic spots, hepatosplenomegaly, hemorrhage, severe necrosis, and atrophic bursa of Fabricius. Based on the local prevalence of the disease, clinical symptoms, and autopsy results, a diagnosis of reovirus infection was made. The affected geese were randomly divided into two groups for isolated care.

[0030] 1 Materials and Methods 1.1 Trial Drugs Chinese medicine granules and Chinese medicine aqueous solution of the present invention 1.2 Experimental animals The sick geese were randomly divided into two groups, experimental group 1 and experimental group 2, with 1000 geese in each group.

[0031] 1.3 Test methods 1.3.1 Add the drug to the feed (mix with water) according to the dosage listed in Table 1 for 7 consecutive days and stop the drug after 7 days.

[0032] Table 1 Experimental groups and drug dosages

[0033] 1.3.2 The two groups of experiments were conducted under the same feeding conditions, and the goose house environment was disinfected daily as required.

[0034] 1.3.3 Observation and measurement items: Observe the changes in the goose flock and their health status daily, and record their feed intake.

[0035] Criteria for judging treatment effect: (1) Effective: The body temperature of the sick goose gradually drops to normal, the spirit, appetite, and feces gradually recover, the sick goose gradually recovers, gradually can stand, and the joint swelling tends to subside; (2) Cured: The body temperature of the sick goose drops to normal, the spirit, appetite, and feces return to normal, the goose can stand, and the joint swelling significantly subsides; (3) Ineffective: After taking the medicine, the symptoms of the sick goose do not improve, and may even worsen or die.

[0036] 2 Results and Analysis 2.1 Clinical manifestations of geese After the treatment period, the symptoms of the geese in test groups 1 and 2 were significantly alleviated, with symptoms such as fever, mental depression, loss of appetite, and diarrhea significantly improved. Their spirits recovered well, and their eating and drinking habits returned to normal. No significant adverse reactions occurred during the treatment. As shown in Table 2, the cure rates and effective numbers for test groups 1 and 2 both reached over 90%, effectively controlling the epidemic and achieving satisfactory therapeutic effects. Other waterfowl, such as ducks, can also be treated with the Chinese medicine preparation of the present invention, achieving similarly good therapeutic effects.

[0037] Table 2 Treatment effects of geese in each group

[0038] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

Claims

1. A Chinese medicinal preparation for treating waterfowl reocolitis, characterized in that: The medicinal preparation is composed of the following raw materials in parts by weight: 20-40 parts of astragalus, 20-40 parts of scutellaria, 10-20 parts of liquorice, 15-30 parts of platycodon, 20-30 parts of epimedium, 10-30 parts of honeysuckle, 10-30 parts of wild chrysanthemum, 20-30 parts of coix seed and 20-35 parts of imperata rhizome.

2. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 1, characterized in that: Astragalus replenishes qi and strengthens the exterior, promotes diuresis and reduces swelling, and enhances the immunity of waterfowl; Scutellaria clears heat and dampness, purges fire and detoxifies, and removes heat and toxins from the bodies of waterfowl; Licorice harmonizes various medicines, clears heat and detoxifies; Platycodon promotes lung function and relieves sore throat, discharges pus and detoxifies; Epimedium warms the kidney and strengthens yang, dispels wind and dampness, and regulates the physiological functions of waterfowl; Honeysuckle and Wild Chrysanthemum clear heat and detoxify, cool blood and reduce swelling; Coix seed promotes diuresis and eliminates dampness, strengthens the spleen and eliminates numbness; Imperata root clears heat and promotes diuresis, cools blood and stops bleeding. These Chinese herbal ingredients work together to treat respiratory soreness in waterfowl.

3. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 2, characterized in that: The preparation method comprises the following steps: step 1: treating scutellaria baicalensis with enzyme: drying the scutellaria baicalensis at 60-80° C. for 2-4 hours, and crushing the scutellaria baicalensis in a prescribed amount to a particle size of less than 3 mm; Mix the crushed Scutellaria baicalensis with water in a weight ratio of 1:15-20, stir evenly, and adjust the pH value to 4.5-6.0; Add 150-250 U of cellulase per gram of Scutellaria baicalensis, add the cellulase to the above mixture, and perform enzymolysis at a temperature of 45-55°C for 3-5 hours; After the enzymatic hydrolysis is completed, the mixed solution is heated to 80-90°C and maintained for 10-15 minutes to terminate the enzyme reaction, and then filtered to obtain the enzyme-treated liquid.

4. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 3, characterized in that: The enzymatically hydrolyzed drug solution is centrifuged at a speed of 3000-5000 rpm for 10-15 minutes to remove impurity particles and improve the clarity of the preparation.

5. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 4, characterized in that: Step 2: Stir-fry coix seed until the surface turns slightly yellow, weigh astragalus, licorice, platycodon, epimedium, honeysuckle, wild chrysanthemum, coix seed and imperata rhizome according to the above ratio, add appropriate amount of water, and soak for 0.5 hours; Add water and decoct twice, the first time adding water in an amount of 8-10 times the total weight of the medicinal materials, decocting for 1-2 hours, the second time adding water in an amount of 6-8 times the total weight of the medicinal materials, decocting for 0.5-1 hour, combining the two decoctions, filtering, adding the medicinal solution treated with the enzyme in step 1, and concentrating to an extract with a relative density of 1.10-1.

25.

6. The Chinese medicinal preparation for treating waterfowl reocolitis and its use according to claim 5, characterized in that: The preparation method also includes an extract purification step: adding 3-5 times the volume of 95% ethanol to the concentrated extract, letting it stand for 24 hours, filtering and recovering the ethanol, so that the purity of the active ingredient is increased by 10-15%.

7. The Chinese medicinal preparation for treating waterfowl reocolitis and its use according to claim 6, characterized in that: Step 3: Spray dry the extract to make powder, or add appropriate amount of auxiliary materials to make granules.

8. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 7, characterized in that: The extract is dissolved in purified water, 0.1-0.5% sodium benzoate is added as a preservative, the pH value is adjusted to 6.0-8.0, filtered through a 0.45μm filter membrane and then filled to remove impurities and improve the clarity of the preparation. The extract is sterilized by moist heat (115°C, 30 minutes) to prepare an oral dosage form.

9. The Chinese medicinal preparation for treating waterfowl reocolitis according to claim 8, characterized in that: After the preparation was made, geese with reocolitis were selected as experimental subjects and randomly divided into two groups; the Chinese medicinal preparation of the present invention was used for treatment, with the drug administered twice a day for 7 consecutive days; After the treatment, the disease condition changes, cure rates, and efficacy rates of the two groups of geese were observed.

10. Use of a traditional Chinese medicine preparation for treating waterfowl reocongestive disease, the traditional Chinese medicine preparation for treating waterfowl reocongestive disease according to any one of claims 1 to 9, characterized in that: The agent is used to treat waterfowl reocongestive disease, relieve the symptoms of waterfowl reocongestive disease, and improve the cure rate and survival rate of waterfowl.

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