Biological reaction device for feed enzymolysis and fermentation and use method of biological reaction device

Abstract

The invention discloses a biological reaction device for feed enzymolysis fermentation and a use method, and relates to the technical field of feed enzymolysis fermentation, and the biological reaction device comprises a tank body, a sealing cover and a partition plate. By means of the fermentation area, the enzymolysis area and the intelligent material circulation assembly, partial backflow and secondary fermentation of enzymolysis products are achieved, the raw material utilization rate and the final product yield are increased, the fermentation area integrates the functions of stirring, temperature control, pH monitoring, gas supply and automatic enzyme inducer adding, the enzyme inducer can be dynamically regulated and input according to pH probe data, and the fermentation efficiency is improved. In the enzymolysis area, a sound wave generator is used for enhancing the enzymolysis reaction efficiency, a second arc-shaped heating block is matched to provide a uniform and appropriate temperature, meanwhile, a filter screen driven by a vibrator is used for separating materials and preventing blockage, the smoothness and high efficiency of the enzymolysis process are guaranteed, and the enzymolysis efficiency is improved. Further, the reaction rate and the product quality are improved, and the energy consumption and the manual operation intensity are reduced.

Description

Technical Field

[0001] The present invention relates to the technical field of feed enzymatic hydrolysis and fermentation, in particular to a bioreactor for feed enzymatic hydrolysis and fermentation and a use method thereof. Background Art

[0002] Feed fermentation is mostly carried out by natural fermentation and fermentation by inoculating commercial lactic acid bacteria and other strains. Feed produced by natural fermentation is generally of unstable quality and is easily infected by miscellaneous bacteria. The feeding effect of pigs varies greatly. With the development of animal husbandry, feed fermentation agents have also been sought after by farmers. People not only pursue pork with better meat quality, but farmers are also looking for feed that can produce high-quality pork.

[0003] The defects of existing feed enzymatic fermentation equipment are: 1. Patent document JP2006050915A discloses a feed additive comprising alginic acid fermentation material and feed. However, the device described in the above document has a technical problem of being unable to carry out logistics circulation, resulting in insufficient conversion of the raw materials and low final product yield; 2. Patent document US20120264178A1 discloses a method for enzymatic hydrolysis and fermentation of low-density biomass using pretreated feed in the presence of enzymes. However, the device in the above document has technical problems such as uneven gas distribution during fermentation, resulting in an unstable dissolved oxygen environment and low utilization rate; 3. Patent document US20150366238A1 discloses a pre-biotic animal feed product. However, the device described in the above document lacks sterilization means, which easily contaminates the core bacterial strains in the fermentation area and damages the fermentation purity. 4. Patent document CN222907918U discloses a feed fermentation enzymatic hydrolysis tank. However, the device in the above document requires manual monitoring and intervention for the reflux of logistics, which has technical problems such as low efficiency and impact on reaction stability. Summary of the Invention

[0004] The purpose of the present invention is to provide a bioreactor for enzymatic hydrolysis and fermentation of feed and a method of use to solve the technical problems raised in the above background technology.

[0005] To achieve the above objectives, the present invention provides the following technical solutions: a bioreactor for enzymatic hydrolysis and fermentation of feed, comprising a tank body, a sealing cover, and a partition, wherein the sealing cover is provided on the top of the tank body, and the partition is fixedly connected to the inner wall of the tank body, wherein the partition separates the tank body into a fermentation zone and an enzymatic hydrolysis zone, wherein the fermentation zone is located above the partition, and the enzymatic hydrolysis zone is located below the partition, and an intelligent material circulation component is provided between the fermentation zone and the enzymatic hydrolysis zone for returning part of the enzymatic hydrolysis product to the fermentation zone for secondary fermentation; The fermentation zone is provided with a fermentation reaction unit and an automatic enzyme inducer addition component. The fermentation reaction unit includes a stirring component, a temperature sensor, a pH probe, a gas supply component and several groups of first arc-shaped heating blocks. The stirring component is used to stir the materials in the fermentation zone. The gas supply component is used to input fermentation gas into the fermentation zone. The temperature sensor and the pH probe are embedded in the side wall of the fermentation zone of the tank body for real-time monitoring of the fermentation environment. The automatic enzyme inducer addition component is used to dynamically regulate the input and stop of the enzyme inducer in response to the data of the pH probe. The first arc-shaped heating block is embedded in the inner wall of the fermentation zone. An enzymatic hydrolysis reaction unit is provided inside the enzymatic hydrolysis zone, and the enzymatic hydrolysis reaction unit includes an acoustic wave generator, several groups of second arc-shaped heating blocks and a filter screen. The acoustic wave generator is attached to the outer wall of the enzymatic hydrolysis zone, and the several groups of second arc-shaped heating blocks are embedded in the inner wall of the enzymatic hydrolysis zone. The outer wall of the filter screen is fixedly installed in the middle of the enzymatic hydrolysis zone, and a vibrator is provided in the middle of the bottom of the filter screen.

[0006] Preferably, the stirring assembly includes a drive motor, and the bottom of the drive motor is installed in the middle of the top of the sealing cover. The output end of the drive motor passes through the sealing cover and is provided with a transmission shaft, and the outer wall of the transmission shaft is installed with several groups of fermentation blades.

[0007] Preferably, the gas delivery assembly includes an air delivery ring, and the air delivery ring is arranged on the inner wall of the fermentation area and laid on the top of the bottom partition of the fermentation area. One side of the top of the air delivery ring is connected to the output end of the air pump through a pipeline passing through the tank body. One end of the air pump is installed on the outer wall of the tank body. An air chamber is provided inside the air delivery ring, and micropores are evenly provided on the annular surface of the top of the air delivery ring for evenly releasing gas.

[0008] Preferably, a transport pipe is provided through the bottom of the partition, and a solenoid valve is provided on the transport pipe. The solenoid valve is electrically connected to a controller, and the controller is installed on the outer wall of the tank.

[0009] Preferably, the intelligent material circulation component includes a reflux pipe, and one end of the reflux pipe is installed through the bottom of the outer wall of the enzymatic hydrolysis area, and the other end is installed through the bottom of the outer wall of the fermentation area. A peristaltic pump is provided on the reflux pipe, and the peristaltic pump is installed on the outer wall of the tank body. A viscosity sensor is provided at one end of the reflux pipe located in the enzymatic hydrolysis area.

[0010] Preferably, the enzyme inducer automatic addition component includes a liquid storage tank, a metering pump and an annular spray pipe, and the bottom of the liquid storage tank is installed on one side of the top of the sealing cover, the metering pump is arranged at the bottom of the outer wall of the liquid storage tank, and the metering pump is electrically connected to the controller, the output end of the metering pump is connected to the input end of the annular spray pipe, and the annular spray pipe is fixedly installed below the sealing cover, and a plurality of nozzles are provided at the bottom of the annular spray pipe, and the output end of the nozzle faces the interior of the fermentation area.

[0011] Preferably, a servo motor is installed at the bottom of the outer wall of the enzymatic hydrolysis zone, and the output end of the servo motor passes through the bottom of the outer wall of the enzymatic hydrolysis zone and is installed with a screw conveyor, and the other end of the bottom of the outer wall of the enzymatic hydrolysis zone passes through a discharge pipe, and one end of the screw conveyor extends to the inner cavity of the discharge pipe.

[0012] Preferably, a microwave sterilization module is embedded in the inner wall of the transport tube, and the microwave sterilization module includes an annular microwave emitter and a shielding cover, and the shielding cover is covered on the outer wall of the transport tube. The annular microwave emitter is automatically activated when the solenoid valve is opened, and the material passing through the transport tube is instantly sterilized.

[0013] Preferably, the working steps of the bioreactor for enzymatic fermentation of feed are as follows: S1. Start the stirring component of the fermentation reaction unit to stir the raw materials, and at the same time start the gas supply component to pass the gas required for fermentation into the fermentation area, and monitor the parameters of the inner wall of the fermentation area in real time through the temperature sensor and pH probe; S2. The controller adjusts the temperature of the fermentation zone through the first arc-shaped heating block according to the data from the temperature sensor to maintain a temperature suitable for fermentation. The enzyme inducer automatic addition component responds to the monitoring data of the pH probe in real time and automatically starts and stops the input of the enzyme inducer. S3. When the fermentation reaction in the fermentation zone reaches a preset stage, the controller issues a command to open the solenoid valve on the transport pipe and activates the microwave sterilization module at the same time. The ring-shaped microwave emitter and the shielding cover kill bacteria as the fermentation product passes through the transport pipe. S4. The material flowing into the enzymatic hydrolysis zone falls on the filter screen, the enzymatic hydrolysis reaction unit is started, and the sound wave generator emits sound waves to the material in the enzymatic hydrolysis zone to promote the contact between the enzyme and the material and improve the enzymatic hydrolysis efficiency. The second arc-shaped heating block is used to heat the logistics in the enzymatic hydrolysis zone to maintain the temperature required for the enzymatic hydrolysis reaction.

[0014] Preferably, the step S2 further includes the following steps: S21. When the pH value reaches the preset threshold for adding enzyme inducer, the component automatically starts to add enzyme inducer to the fermentation zone; S22. When the pH value reaches the preset threshold for stopping the enzyme inducer, the component automatically stops adding; The step S4 also includes the following steps: S41. The vibrator is started to drive the filter to vibrate continuously, which is used to prevent the material flow from caking on the filter and promote the small molecule enzymatic hydrolysis products to pass through the filter.

[0015] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention achieves partial reflux and secondary fermentation of enzymatic hydrolysis products through the fermentation zone, enzymatic hydrolysis zone, and intelligent material circulation components, thereby improving raw material utilization and final product yield. The fermentation zone integrates stirring, temperature control, pH monitoring, gas supply, and automatic enzyme inducer addition functions. The enzyme inducer can be dynamically controlled based on pH probe data to ensure a precise and stable fermentation environment, optimize fermentation efficiency and product quality consistency. The enzymatic hydrolysis zone uses an acoustic wave generator to enhance the efficiency of the enzymatic hydrolysis reaction, and cooperates with a second arc-shaped heating block to provide a uniform and appropriate temperature. At the same time, a vibrator-driven filter separates materials and prevents clogging, ensuring a smooth and efficient enzymatic hydrolysis process, thereby improving reaction rate and product quality, and reducing energy consumption and manual operation intensity. 2. The present invention uses a drive motor to drive a transmission shaft to drive the fermentation blades for three-dimensional stirring, ensuring that the materials in the fermentation area are fully and evenly mixed, eliminating dead corners, and promoting effective contact between microorganisms and substrates and mass transfer efficiency. The gas transmission component adopts a ring design. The gas transmission ring is laid on the top of the bottom partition of the fermentation area. The internal air chamber releases gas through micropores evenly distributed on the top. Combined with the air supply of an external air pump, the gas is evenly and diffusely distributed from the bottom of the fermentation area, providing a stable and evenly distributed dissolved oxygen environment for the microorganisms, thereby avoiding local hypoxia or gas short-circuiting, improving the gas utilization rate and reaction rate of the fermentation process, and ensuring the uniformity and efficiency of the fermentation environment. 3. The present invention utilizes a microwave sterilization module integrated into the inner wall of the transport tube. The annular microwave emitter is automatically activated when the solenoid valve opens to allow material flow. Combined with the shielding cover on the outer wall, this module provides instantaneous and efficient microwave sterilization of the passing material, preventing contaminants from entering the fermentation area with recirculated material, thereby ensuring the purity of the core fermentation strain and the safety of the fermentation process. 4. The present invention realizes efficient and controllable material circulation through the design of the reflux pipe. The two ends of the reflux pipe respectively pass through the bottom of the enzymatic hydrolysis area and the bottom of the fermentation area, ensuring that the material at the bottom of the enzymatic hydrolysis area can be effectively extracted. The peristaltic pump is installed on the outer wall of the tank to drive the flow of material in the pipe. Then, through the setting of the viscosity sensor, the viscosity change of the material to be refluxed can be monitored in real time. When the viscosity of the material does not meet the required requirements, the operation of the peristaltic pump can be automatically triggered or adjusted to perform secondary fermentation, thereby improving the efficiency and stability of the entire fermentation-enzymatic hydrolysis synergistic reaction. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 It is a three-dimensional schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the cross-sectional structure of the tank body of the present invention; Figure 3 Schematic diagram of the enzymatic hydrolysis zone structure of the present invention; Figure 4 This is a schematic diagram of the fermentation zone structure of the present invention; Figure 5This is a schematic diagram of the gas transmission ring structure of the present invention; Figure 6 Schematic diagram of the system flow of the present invention; Figure 7 Schematic diagram of the workflow of the present invention.

[0017] In the figure: 1. tank body; 2. sealing cover; 3. partition; 4. fermentation area; 5. enzymolysis area; 6. fermentation reaction unit; 7. temperature sensor; 8. pH probe; 9. first arc-shaped heating block; 10. enzymolysis reaction unit; 11. sound wave generator; 12. second arc-shaped heating block; 13. filter; 14. vibrator; 15. drive motor; 16. transmission shaft; 17. fermentation blade; 18. air supply ring; 19. air pump; 20. air chamber; 21. transport pipe; 22. solenoid valve; 23. controller; 24. return pipe; 25. peristaltic pump; 26. viscosity sensor; 27. liquid storage tank; 28. metering pump; 29. annular spray pipe; 30. nozzle; 31. servo motor; 32. screw conveyor; 33. discharge pipe; 35. annular microwave transmitter; 36. shielding cover. DETAILED DESCRIPTION

[0018] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0019] In the description of the present invention, it should be noted that the terms "upper," "lower," "inner," "outer," "front end," "rear end," "both ends," "one end," "the other end," and the like, indicating orientations or positional relationships, are based on the orientations or positional relationships shown in the accompanying drawings and are intended solely to facilitate and simplify the description of the present invention. They are not intended to indicate or imply that the devices or components referred to must have, be constructed, or operate in a specific orientation, and therefore should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance.

[0020] In the description of the present invention, it should be noted that, unless otherwise expressly specified or limited, the terms "installed," "provided with," "connected," etc., should be understood in a broad sense. For example, "connected" may refer to a fixed connection, a detachable connection, or an integral connection; it may refer to a mechanical connection or an electrical connection; it may refer to a direct connection or an indirect connection through an intermediate medium; it may refer to internal communication between two components. Those skilled in the art will be able to understand the specific meanings of the above terms in the present invention based on specific circumstances.

[0021] Example 1: Please refer to Figure 1 、 Figure 2 and Figure 6 The present invention provides an embodiment of a bioreactor for enzymatic hydrolysis and fermentation of feed, comprising a tank body 1, a sealing cover 2 and a partition 3. The sealing cover 2 is provided on the top of the tank body 1, and the partition 3 is fixedly connected to the inner wall of the tank body 1. The partition 3 separates the tank body 1 into a fermentation area 4 and an enzymatic hydrolysis area 5, and the fermentation area 4 is located above the partition 3, and the enzymatic hydrolysis area 5 is located below the partition 3. An intelligent material circulation component is provided between the fermentation area 4 and the enzymatic hydrolysis area 5 for returning part of the enzymatic hydrolysis product to the fermentation area 4 for secondary fermentation. A fermentation reaction unit 6 and an automatic enzyme inducer addition component are provided inside the fermentation area 4. The fermentation reaction unit 6 includes a stirring component, a temperature sensor 7, a pH probe 8, a gas supply component and several groups of first arc-shaped heating blocks 9. The stirring component is used to stir the material in the fermentation area 4, and the gas supply component is used to input fermentation gas into the fermentation area 4. The temperature sensor 7 and the pH probe 8 are embedded in the side wall of the fermentation area 4 of the tank body 1 for real-time monitoring of the fermentation environment. The automatic enzyme inducer addition component is used to dynamically control the input and stop of the enzyme inducer in response to the data of the pH probe 8. The first arc-shaped heating block 9 is embedded in the inner wall of the fermentation area 4; An enzymatic reaction unit 10 is provided inside the enzymatic hydrolysis zone 5. The enzymatic hydrolysis reaction unit 10 includes an acoustic wave generator 11, several groups of second arc-shaped heating blocks 12 and a filter 13. The acoustic wave generator 11 is attached to the outer wall of the enzymatic hydrolysis zone 5, and several groups of second arc-shaped heating blocks 12 are embedded in the inner wall of the enzymatic hydrolysis zone 5. The outer wall of the filter 13 is fixedly installed in the middle of the enzymatic hydrolysis zone 5, and a vibrator 14 is provided in the middle of the bottom of the filter 13. Furthermore, through the fermentation zone 4, the enzymatic hydrolysis zone 5 and the intelligent material circulation component, partial reflux and secondary fermentation of the enzymatic hydrolysis product are achieved, thereby improving the raw material utilization rate and the final product yield. The fermentation zone 4 integrates stirring, temperature control, pH monitoring, gas supply and automatic addition of enzyme inducers. The enzyme inducer can be dynamically adjusted according to the data of the pH probe 8 to ensure that the fermentation environment is accurate and stable, and optimize the fermentation efficiency and product quality consistency. The enzymatic hydrolysis zone 5 uses the sound wave generator 11 to enhance the enzymatic hydrolysis reaction efficiency, and cooperates with the second arc-shaped heating block 12 to provide a uniform and appropriate temperature. At the same time, the filter screen 13 driven by the vibrator 14 separates the material and prevents blockage, ensuring the smoothness and efficiency of the enzymatic hydrolysis process, thereby improving the reaction rate and product quality, and reducing energy consumption and manual operation intensity.

[0022] Example 2: Please refer to Figure 1 、 Figure 2 、 Figure 4 and Figure 5The present invention provides an embodiment in which the stirring assembly includes a driving motor 15, wherein the bottom of the driving motor 15 is mounted in the middle of the top of the sealing cover 2, the output end of the driving motor 15 passes through the sealing cover 2 and is provided with a transmission shaft 16, and the outer wall of the transmission shaft 16 is mounted with a plurality of fermentation blades 17; The gas delivery assembly includes a gas delivery ring 18, which is arranged on the inner wall of the fermentation area 4 and laid on the top of the partition 3 at the bottom of the fermentation area 4. One side of the top of the gas delivery ring 18 is connected to the output end of the air pump 19 through a pipeline passing through the tank body 1. One end of the air pump 19 is installed on the outer wall of the tank body 1. An air chamber 20 is provided inside the gas delivery ring 18. The annular surface of the top of the gas delivery ring 18 is evenly provided with micropores for uniformly releasing gas. Furthermore, the drive motor 15 drives the transmission shaft 16 to drive the fermentation blades 17 for three-dimensional stirring, ensuring that the materials in the fermentation area 4 are fully and evenly mixed, eliminating dead corners, and promoting effective contact and mass transfer efficiency between microorganisms and substrates. The gas supply component adopts a ring design, and the gas supply ring 18 is laid on the top of the bottom partition 3 of the fermentation area 4. Its internal air chamber 20 releases gas through micropores evenly distributed on the top. Combined with the air supply of the external air pump 19, the gas is evenly and diffusely distributed from the bottom of the fermentation area 4, providing a stable and evenly distributed dissolved oxygen environment for microorganisms, thereby avoiding local hypoxia or gas short-circuiting, improving the gas utilization rate and reaction rate of the fermentation process, and ensuring the uniformity and efficiency of the fermentation environment.

[0023] Example 3: Please refer to Figure 1 、 Figure 2 and Figure 5 In one embodiment of the present invention, a transport pipe 21 is provided through the bottom of the partition 3, and a solenoid valve 22 is provided on the transport pipe 21. The solenoid valve 22 is electrically connected to a controller 23, and the controller 23 is installed on the outer wall of the tank body 1; A microwave sterilization module is embedded in the inner wall of the transport tube 21. The microwave sterilization module includes an annular microwave emitter 35 and a shielding cover 36. The shielding cover 36 is covered on the outer wall of the transport tube 21. The annular microwave emitter 35 is automatically activated when the solenoid valve 22 is opened, and the material passing through the transport tube 21 is instantly sterilized. Furthermore, through the microwave sterilization module integrated in the inner wall of the transport pipe 21, the annular microwave emitter 35 is automatically activated when the solenoid valve 22 opens the material flow. In conjunction with the shielding cover 36 on the outer wall, the passing material can be subjected to instantaneous and efficient microwave sterilization treatment, thereby preventing miscellaneous bacteria from entering with the reflux material and contaminating the fermentation area 4, thereby ensuring the purity of the core fermentation strain and the safety of the fermentation process.

[0024] Example 4: Please refer to Figure 1 and Figure 3The present invention provides an embodiment in which the intelligent material circulation component includes a reflux pipe 24, one end of which is installed through the bottom of the outer wall of the enzymatic hydrolysis zone 5, and the other end of which is installed through the bottom of the outer wall of the fermentation zone 4. A peristaltic pump 25 is provided on the reflux pipe 24, and the peristaltic pump 25 is installed on the outer wall of the tank body 1. A viscosity sensor 26 is provided at one end of the reflux pipe 24 located in the enzymatic hydrolysis zone 5. Furthermore, efficient and controllable material circulation is achieved through the design of the reflux pipe 24. The two ends of the reflux pipe 24 respectively pass through the bottom of the enzymatic hydrolysis zone 5 and the bottom of the fermentation zone 4, ensuring that the material at the bottom of the enzymatic hydrolysis zone 5 can be effectively extracted. The peristaltic pump 25 is installed on the outer wall of the tank body 1 to drive the flow of material in the pipe. The viscosity sensor 26 is then set to monitor the viscosity changes of the material to be refluxed in real time. When the viscosity of the material does not meet the required requirements, the operation of the peristaltic pump 25 can be automatically triggered or adjusted to perform secondary fermentation, thereby improving the efficiency and stability of the entire fermentation-enzymatic hydrolysis synergistic reaction.

[0025] Example 5: Please refer to Figure 1 、 Figure 2 and Figure 5 , the present invention provides an embodiment: the enzyme inducer automatic addition component includes a liquid storage tank 27, a metering pump 28 and an annular spray pipe 29, and the bottom of the liquid storage tank 27 is installed on one side of the top of the sealing cover 2, the metering pump 28 is arranged at the bottom of the outer wall of the liquid storage tank 27, and the metering pump 28 is electrically connected to the controller 23, the output end of the metering pump 28 is connected to the input end of the annular spray pipe 29, and the annular spray pipe 29 is fixedly installed below the sealing cover 2, and a plurality of nozzles 30 are provided at the bottom of the annular spray pipe 29, and the output end of the nozzle 30 faces the interior of the fermentation area 4; Furthermore, the structural design of the automatic enzyme inducer addition component realizes accurate, uniform and automated enzyme inducer addition. The enzyme inducer is quantitatively delivered to the annular spray pipe 29 fixed under the sealing cover 2 through the metering pump 28. Through the multiple nozzles 30 evenly distributed at its bottom, the enzyme inducer is sprayed to cover the upper space of the entire fermentation area 4, which is conducive to the enzyme inducer being evenly dispersed from top to bottom on the entire surface of the fermentation material, avoiding local concentrations that are too high or too low, ensuring consistent microbial contact, and realizing dynamic start and stop and dosage adjustment of enzyme inducer addition through feedback signals from the pH probe 8, without the need for manual intervention, thereby improving fermentation efficiency and product quality.

[0026] Example 6: Please refer to Figure 1 、 Figure 2 and Figure 3In one embodiment of the present invention, a servo motor 31 is installed at the bottom of the outer wall of the enzymatic hydrolysis zone 5. The output end of the servo motor 31 passes through the bottom of the outer wall of the enzymatic hydrolysis zone 5 and is installed with a screw conveyor 32. The other end of the bottom of the outer wall of the enzymatic hydrolysis zone 5 passes through a discharge pipe 33. One end of the screw conveyor 32 extends to the inner cavity of the discharge pipe 33. Furthermore, the screw conveyor 32 is driven by the servo motor 31, and its power output is precisely controllable, and the conveying speed and strength can be flexibly adjusted according to the material characteristics and process requirements. The screw conveyor 32 penetrates into the bottom of the enzymatic hydrolysis zone 5, and is suitable for efficiently conveying viscous or high-solid enzymatic hydrolysis products. Its rotary pushing method effectively overcomes the problems of material sticking to the wall and deposition, ensuring that the bottom material is thoroughly and smoothly emptied and conveyed. The output end of the screw conveyor 32 extends directly to the inner cavity of the discharge pipe 33, realizing seamless and directional transfer of materials from the enzymatic hydrolysis zone 5 to the discharge pipe 33, minimizing the risk of residue and blockage, thereby not only ensuring the efficient and thorough discharge of the enzymatic hydrolysis products, but also improving the degree of automation and reliability of the operation of the entire device.

[0027] Example 7: Please refer to Figure 7 The present invention provides an embodiment in which the working steps of the bioreactor for enzymatic fermentation of feed are as follows: S1, start the stirring component of the fermentation reaction unit 6 to stir the raw materials, and at the same time start the gas supply component to pass the gas required for fermentation into the fermentation area 4, and monitor the parameters of the inner wall of the fermentation area 4 in real time through the temperature sensor 7 and the pH probe 8; S2, the controller 23 adjusts the temperature of the fermentation zone 4 through the first arc-shaped heating block 9 according to the data of the temperature sensor 7 to maintain a temperature suitable for fermentation. The enzyme inducer automatic addition component responds to the monitoring data of the pH probe 8 in real time and automatically starts and stops the input of the enzyme inducer; S3: When the fermentation reaction in the fermentation zone 4 reaches a preset stage, the controller 23 issues a command to open the solenoid valve 22 on the transport pipe 21 and activates the microwave sterilization module at the same time as the solenoid valve 22 opens. The annular microwave emitter 35 and the shielding cover 36 kill bacteria as the fermentation product passes through the transport pipe 21. S4, the material flowing into the enzymatic hydrolysis zone 5 falls on the filter 13, the enzymatic hydrolysis reaction unit 10 is started, the sound wave generator 11 emits sound waves to the material in the enzymatic hydrolysis zone 5, promotes the contact between the enzyme and the material, and improves the enzymatic hydrolysis efficiency. The second arc-shaped heating block 12 is used to heat the logistics in the enzymatic hydrolysis zone 5 to maintain the temperature required for the enzymatic hydrolysis reaction; S5, the two ends of the reflux pipe 24 respectively pass through the bottom of the enzymatic hydrolysis area 5 and the bottom of the fermentation area 4 to ensure that the material at the bottom of the enzymatic hydrolysis area 5 can be effectively extracted. The peristaltic pump 25 is installed on the outer wall of the tank body 1 to drive the flow of the material in the pipe. The viscosity sensor 26 is set to monitor the viscosity change of the material to be refluxed in real time. When the viscosity of the material does not meet the required requirements, the operation of the peristaltic pump 25 can be automatically triggered or adjusted to perform secondary fermentation. Step S1 also includes the following steps: S11, the drive motor 15 drives the transmission shaft 16 to drive the fermentation blades 17 to perform three-dimensional stirring, ensuring that the materials in the fermentation zone 4 are fully and evenly mixed, eliminating dead corners, and promoting effective contact between microorganisms and substrates and mass transfer efficiency; Step S2 also includes the following steps: S21. When the pH value reaches the preset threshold for adding enzyme inducer, the component automatically starts to add enzyme inducer to the fermentation zone 4; S22. When the pH value reaches the preset threshold for stopping the enzyme inducer, the component automatically stops adding; Step S4 also includes the following steps: S41 , the vibrator 14 is started to drive the filter 13 to vibrate continuously, so as to prevent the material flow from being condensed on the filter 13 and to promote the small molecule enzymatic hydrolysis products to pass through the filter 13 .

[0028] Working principle: Through the fermentation zone 4, enzymatic hydrolysis zone 5 and intelligent material circulation components, partial reflux and secondary fermentation of enzymatic hydrolysis products are achieved, thereby improving raw material utilization and final product yield. The fermentation zone 4 integrates stirring, temperature control, pH monitoring, gas supply and automatic addition of enzyme inducers. The enzyme inducer can be dynamically adjusted according to the data of the pH probe 8 to ensure that the fermentation environment is accurate and stable, and optimize the fermentation efficiency and product quality consistency. The enzymatic hydrolysis zone 5 uses the sound wave generator 11 to enhance the efficiency of the enzymatic hydrolysis reaction, and cooperates with the second arc-shaped heating block 12 to provide a uniform and appropriate temperature. At the same time, the filter 13 driven by the vibrator 14 separates the material and prevents clogging, ensuring the smoothness and efficiency of the enzymatic hydrolysis process, thereby improving the reaction rate and product quality, and reducing energy consumption and manual operation intensity. The driving motor 15 drives the transmission shaft 16 to drive the fermentation blades 17 for three-dimensional stirring, ensuring that the materials in the fermentation area 4 are fully and evenly mixed, eliminating dead corners, promoting effective contact between microorganisms and substrates and mass transfer efficiency, and the gas delivery component adopts a ring design. The gas delivery ring 18 is laid on the top of the partition 3 at the bottom of the fermentation area 4, and its internal air chamber 20 releases gas through the micropores evenly distributed on the top. Combined with the air supply of the external air pump 19, the gas is evenly and diffusely distributed from the bottom of the fermentation area 4, providing a stable and evenly distributed dissolved oxygen environment for microorganisms, thereby avoiding local hypoxia or gas short-circuiting, improving the gas utilization rate and reaction rate of the fermentation process, and ensuring the uniformity and efficiency of the fermentation environment. Through the microwave sterilization module integrated on the inner wall of the transport pipe 21, the annular microporous The wave emitter 35 is automatically activated when the solenoid valve 22 opens the material flow. In conjunction with the shielding cover 36 on the outer wall, it can perform instantaneous and efficient microwave sterilization on the passing material, thereby preventing miscellaneous bacteria from entering the fermentation area 4 with the reflux material and contaminating the fermentation area 4, ensuring the purity of the core fermentation bacteria and the safety of the fermentation process. The design of the reflux pipe 24 realizes efficient and controllable material circulation. The two ends of the reflux pipe 24 respectively pass through the bottom of the enzymatic hydrolysis area 5 and the bottom of the fermentation area 4 to ensure that the material at the bottom of the enzymatic hydrolysis area 5 can be effectively extracted. The peristaltic pump 25 is installed on the outer wall of the tank body 1 to drive the flow of material in the pipe. The viscosity sensor 26 is then set to monitor the viscosity change of the reflux material in real time. When the viscosity of the material does not meet the required requirements, the peristaltic pump 25 can be automatically triggered or adjusted. The operation of the fermentation chamber 2 is carried out to carry out secondary fermentation, thereby improving the efficiency and stability of the entire fermentation-enzymolysis synergistic reaction. The structural design of the automatic enzyme inducer addition component realizes the precise, uniform and automatic addition of enzyme inducers. The enzyme inducer is quantitatively delivered to the annular spray pipe 29 fixed under the sealing cover 2 by the metering pump 28. Through the multiple nozzles 30 evenly distributed at the bottom of the spray pipe 29, the enzyme inducer is sprayed to cover the upper space of the entire fermentation area 4, which is conducive to the enzyme inducer being evenly dispersed from top to bottom to the entire surface of the fermentation material, avoiding local concentrations that are too high or too low, ensuring consistent contact of microorganisms, and realizing dynamic start and stop and dosage adjustment of enzyme inducer addition through feedback signals from the pH probe 8, without manual intervention, thereby improving fermentation efficiency and product quality.The servo motor 31 drives the screw conveyor 32, whose power output is precisely controllable and can flexibly adjust the conveying speed and force according to the material characteristics and process requirements. The screw conveyor 32 penetrates deep into the bottom of the enzymatic hydrolysis zone 5, suitable for efficiently conveying viscous or high-solids enzymatic hydrolysis products. Its rotary pushing method effectively overcomes the problems of material sticking to the wall and sedimentation, ensuring that the bottom material is completely and smoothly emptied and conveyed. The output end of the screw conveyor 32 extends directly into the lumen of the discharge pipe 33, achieving seamless and directional transfer of materials from the enzymatic hydrolysis zone 5 to the discharge pipe 33, minimizing the risk of residue and blockage. This not only ensures the efficient and complete discharge of the enzymatic hydrolysis products, but also improves the automation and reliability of the entire device operation.

[0029] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the invention can be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. Therefore, the embodiments should be considered in all respects as illustrative and non-restrictive, and the scope of the invention is defined by the appended claims, not the foregoing description, and all variations within the meaning and range of equivalents of the claims are intended to be included therein. Any reference sign in a claim should not be construed as limiting the claim to which it relates.

Claims

1. A bioreactor for enzymatic fermentation of feed, comprising a tank body (1), a sealing cover (2) and a partition (3), characterized in that: A sealing cover (2) is provided on the top of the tank body (1), and a partition (3) is fixedly connected to the inner wall of the tank body (1), wherein the partition (3) separates the tank body (1) into a fermentation area (4) and an enzymolysis area (5), wherein the fermentation area (4) is located above the partition (3) and the enzymolysis area (5) is located below the partition (3), and an intelligent material circulation component is provided between the fermentation area (4) and the enzymolysis area (5) for returning part of the enzymolysis product to the fermentation area (4) for secondary fermentation; A fermentation reaction unit (6) and an enzyme inducer automatic addition component are provided inside the fermentation zone (4). The fermentation reaction unit (6) includes a stirring component, a temperature sensor (7), a pH probe (8), a gas supply component and a plurality of first arc-shaped heating blocks (9). The stirring component is used to stir the material in the fermentation zone (4). The gas supply component is used to input fermentation gas into the fermentation zone (4). The temperature sensor (7) and the pH probe (8) are embedded in the side wall of the fermentation zone (4) of the tank body (1) for real-time monitoring of the fermentation environment. The enzyme inducer automatic addition component is used to dynamically regulate the input and stop of the enzyme inducer in response to the data of the pH probe (8). The first arc-shaped heating block (9) is embedded in the inner wall of the fermentation zone (4). An enzymatic hydrolysis reaction unit (10) is provided inside the enzymatic hydrolysis zone (5), and the enzymatic hydrolysis reaction unit (10) includes a sound wave generator (11), a plurality of groups of second arc-shaped heating blocks (12) and a filter screen (13). The sound wave generator (11) is attached to the outer wall of the enzymatic hydrolysis zone (5), the plurality of groups of second arc-shaped heating blocks (12) are embedded in the inner wall of the enzymatic hydrolysis zone (5), the outer wall of the filter screen (13) is fixedly installed in the middle of the enzymatic hydrolysis zone (5), and a vibrator (14) is provided in the middle of the bottom of the filter screen (13).

2. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: The stirring assembly includes a driving motor (15), and the bottom of the driving motor (15) is installed in the middle of the top of the sealing cover (2). The output end of the driving motor (15) passes through the sealing cover (2) and is provided with a transmission shaft (16). The outer wall of the transmission shaft (16) is installed with a plurality of groups of fermentation blades (17).

3. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: The gas delivery assembly includes a gas delivery ring (18), and the gas delivery ring (18) is arranged on the inner wall of the fermentation area (4) and laid on the top of the bottom partition (3) of the fermentation area (4). One side of the top of the gas delivery ring (18) is connected to the output end of the air pump (19) through a pipeline penetrating the tank body (1). One end of the air pump (19) is installed on the outer wall of the tank body (1). An air chamber (20) is provided inside the gas delivery ring (18), and micropores are evenly provided on the annular surface of the top of the gas delivery ring (18) for evenly releasing gas.

4. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: A transport pipe (21) is provided through the bottom of the partition (3), and a solenoid valve (22) is provided on the transport pipe (21). The solenoid valve (22) is electrically connected to a controller (23), and the controller (23) is installed on the outer wall of the tank body (1).

5. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: The intelligent material circulation component includes a reflux pipe (24), one end of which is installed through the bottom of the outer wall of the enzymolysis zone (5), and the other end of which is installed through the bottom of the outer wall of the fermentation zone (4). A peristaltic pump (25) is provided on the reflux pipe (24), and the peristaltic pump (25) is installed on the outer wall of the tank body (1). A viscosity sensor (26) is provided at one end of the reflux pipe (24) located in the enzymolysis zone (5).

6. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: The enzyme inducer automatic addition component includes a liquid storage tank (27), a metering pump (28) and an annular spray pipe (29), and the bottom of the liquid storage tank (27) is installed on one side of the top of the sealing cover (2), the metering pump (28) is arranged at the bottom of the outer wall of the liquid storage tank (27), and the metering pump (28) is electrically connected to the controller (23), the output end of the metering pump (28) is connected to the input end of the annular spray pipe (29), and the annular spray pipe (29) is fixedly installed below the sealing cover (2), and a plurality of nozzles (30) are provided at the bottom of the annular spray pipe (29), and the output end of the nozzle (30) faces the inside of the fermentation area (4).

7. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 1, characterized in that: A servo motor (31) is installed at the bottom of the outer wall of the enzymolysis zone (5), and an output end of the servo motor (31) penetrates the bottom of the outer wall of the enzymolysis zone (5) and is installed with a screw conveyor (32). The other end of the bottom of the outer wall of the enzymolysis zone (5) penetrates and is installed with a discharge pipe (33), and one end of the screw conveyor (32) extends to the inner cavity of the discharge pipe (33).

8. The bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 4, characterized in that: A microwave sterilization module is embedded in the inner wall of the transport tube (21), and the microwave sterilization module includes an annular microwave emitter (35) and a shielding cover (36), and the shielding cover (36) is covered on the outer wall of the transport tube (21). The annular microwave emitter (35) is automatically activated when the solenoid valve (22) is opened, and the material passing through the transport tube (21) is instantly sterilized.

9. The method for using the bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 8, characterized in that: The working steps of the bioreactor for enzymatic fermentation of feed are as follows: S1, starting the stirring component of the fermentation reaction unit (6) to stir the raw materials, and at the same time starting the gas supply component to pass the gas required for fermentation into the fermentation area (4), and monitoring the parameters of the inner wall of the fermentation area (4) in real time through the temperature sensor (7) and the pH probe (8); S2, the controller (23) adjusts the temperature of the fermentation zone (4) through the first arc-shaped heating block (9) according to the data of the temperature sensor (7) to maintain a temperature suitable for fermentation, and the enzyme inducer automatic addition component responds to the monitoring data of the pH probe (8) in real time to automatically start and stop the input of the enzyme inducer; S3, when the fermentation reaction in the fermentation zone (4) reaches a preset stage, the controller (23) issues a command to open the solenoid valve (22) on the transport pipe (21), and activates the microwave sterilization module at the same time as the solenoid valve (22) is opened, and kills bacteria when the fermentation product passes through the transport pipe (21) through the annular microwave emitter (35) and the shielding cover (36); S4. The material flowing into the enzymatic hydrolysis zone (5) falls on the filter (13), the enzymatic hydrolysis reaction unit (10) is started, and the sound wave generator (11) emits sound waves to the material in the enzymatic hydrolysis zone (5), promoting the contact between the enzyme and the material and improving the enzymatic hydrolysis efficiency. The second arc-shaped heating block (12) is used to heat the logistics in the enzymatic hydrolysis zone (5) to maintain the temperature required for the enzymatic hydrolysis reaction.

10. The method for using the bioreactor for enzymatic hydrolysis and fermentation of feed according to claim 9, characterized in that: The step S2 also includes the following steps: S21, when the pH value reaches a preset threshold for adding enzyme inducer, the component automatically starts to add enzyme inducer to the fermentation zone (4); S22. When the pH value reaches the preset threshold for stopping the enzyme inducer, the component automatically stops adding; The step S4 also includes the following steps: S41, the vibrator (14) is started to drive the filter (13) to vibrate continuously, so as to prevent the material flow from being condensed on the filter (13) and to promote the small molecule enzymatic hydrolysis products to pass through the filter (13).

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