Mesenchymal stem cell culture and separation device
By forming a vortex in the culture dish to rinse and collect the mesenchymal stem cell culture separation device with lower active cells, the problem of doping between lower active cells and high cells is solved, and efficient separation and extraction of active cells is achieved.
Patent Information
- Application Number
- CN202510457186.1
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-13
- Publication Date
- 2025-08-19
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, during the culture process of mesenchymal stem cell, cells with lower activity are prone to adhere to the inner wall and partition plate of the culture vessel, and doped with cells with higher activity, making subsequent separation difficult.
A mesenchymal stem cell culture separation device was designed to form a vortex through the filter drainage mechanism and the suction mechanism, and the cells with lower activeness on the inner wall of the culture dish and the attachment plate were washed and collected. The old culture medium was discharged using the filter drainage mechanism and the drainage valve to ensure that only cells with higher activeness were left.
The efficient separation of cells with lower activity is achieved, and subsequent operations are simplified, ensuring that only cells with higher activity are attached to the inner walls of the culture dish and the attachment plate, which is convenient for subsequent cell extraction.
Smart Images
Figure CN120505196A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of cell culture, and in particular to a mesenchymal stem cell culture and separation device. Background Art
[0002] Mesenchymal stem cells are a type of pluripotent stem cell that possess all the common properties of stem cells, namely self-renewal and multidirectional differentiation. They are also the most widely used in clinical applications. Their combined use with hematopoietic stem cells can improve transplant success rates and accelerate hematopoietic reconstitution. Existing techniques sometimes involve adding dividers to the culture vessel to increase the surface area within the culture medium where cells can attach.
[0003] However, a portion of cells with low activity in the culture medium will attach to the inner wall of the culture vessel and the partition plate. These cells with low activity will be mixed with cells with high activity attached to the inner wall of the culture vessel and the partition plate. It is difficult to separate these cells with low activity subsequently. Therefore, a mesenchymal stem cell culture and separation device is proposed. Summary of the Invention
[0004] In order to solve the shortcomings of the prior art, the present invention proposes a mesenchymal stem cell culture and separation device.
[0005] To achieve the above-mentioned objectives, the present invention adopts the following technical solution: a mesenchymal stem cell culture and separation device, comprising a culture dish, a dish cover detachably mounted on the top of the culture dish, a filtering and draining mechanism mounted on the dish cover, a suction mechanism rotatably mounted inside the culture dish, and a plurality of attachment plates rotatably mounted on the inner wall of the bottom of the culture dish in a circular array, the filtering and draining mechanism being used to drive the suction mechanism to form a vortex in the culture fluid inside the culture dish, which can evenly distribute the culture fluid in the culture dish and flush out cells with lower activity on the inner wall of the culture dish and on the plurality of attachment plates through the vortex, and the flushed-out cells with lower activity are collected by the filtering and draining mechanism, a drain valve is provided through the culture dish, the filtering and draining mechanism and the drain valve cooperate to drain out the old culture fluid in the culture dish, a filling pipe is fixedly mounted through the dish cover, a base is fixedly mounted on the bottom of the culture dish, and a synchronization mechanism for causing the plurality of attachment plates to move synchronously is installed in the base.
[0006] Preferably, the filtering and drainage mechanism includes a motor fixedly mounted on the top of the dish cover, the motor rotates the output shaft to rotate and penetrate the dish cover, and a transmission shaft is fixedly mounted on the end of the output shaft of the motor, the bottom end of the transmission shaft is detachably mounted with an annular box 1, the bottom of the annular box 1 is detachably mounted with a cylinder, the suction mechanism includes an arc-shaped blade rotatably connected to the bottom inner wall of the culture dish, a plurality of circular plates distributed in a circular array are fixedly mounted on the top of the plurality of circular plates, and the same annular plate is fixedly mounted on the top of the multiple circular plates, and the annular plate is fixedly sleeved on the cylinder.
[0007] Preferably, the top of the cylinder is flush with the bottom inner wall of the annular box one, a plurality of liquid outlets distributed in a circular array are provided on the inner wall of the annular box one, and a filter cartridge is detachably mounted on the inner wall of the annular box one.
[0008] Preferably, a conical cylinder is fixedly mounted on the top end of the cylinder.
[0009] Preferably, the filtering and drainage mechanism also includes a plurality of guide rods passing through the dish cover and slidingly connected to the dish cover, the plurality of guide rods are distributed in a circular array, the bottom ends of the plurality of guide rods are fixedly mounted with the same annular box 2, the annular box 2 is coaxially arranged with the annular box, and the inner wall of the annular box 2 is open, and the inner diameter of the annular box 2 is equal to the outer diameter of the annular box 1, the top ends of the plurality of guide rods are fixedly mounted with the same pressure ring, the sliding sleeve on the guide rod is provided with a spring, and the two ends of the spring are respectively fixedly connected to the annular box 2 and the dish cover.
[0010] Preferably, the filtering and draining mechanism also includes a drain pipe fixedly connected to the inner wall of the drain valve, the drain pipe is L-shaped, and a liquid outlet is provided on the bottom inner wall of the annular box 2. The liquid outlet is coaxially arranged with the end of the drain pipe away from the drain valve, and the inner diameter of the liquid outlet is equal to the outer diameter of the drain pipe.
[0011] Preferably, a circular shaft is fixedly installed on the attachment plate, the circular shaft passes through the bottom of the culture dish and is rotatably connected to the culture dish, the bottom end of the circular shaft extends into the base, the synchronization mechanism includes a plurality of gears fixedly installed on the bottom ends of the plurality of circular shafts, and the plurality of gears are meshed with the same gear ring, the synchronization mechanism also includes a cylinder fixedly installed on the bottom of the culture dish, the cylinder is coaxially arranged with the culture dish, and the gear ring is rotatably sleeved on the cylinder.
[0012] Preferably, the bottom of the gear ring is rotatably mounted with a plurality of rotating shafts 1 distributed in a circular array, and the bottom of the culture dish is rotatably mounted with a plurality of rotating shafts 2 distributed in a circular array. The number of rotating shafts 1 and 2 is equal, and the adjacent rotating shafts 2 and 1 are fixedly mounted with the same tension spring.
[0013] Compared with the prior art, the present invention has the following beneficial effects: 1. The present invention forms a vortex in the culture fluid inside the culture dish through the cooperation of the filtration and drainage mechanism and the suction mechanism. The vortex continuously flushes the inner wall of the culture dish and the multiple attachment plates, thereby flushing away cells with low activity attached to the inner wall of the culture dish and the multiple attachment plates, ensuring that the cells attached to the culture dish and the multiple attachment plates are all high-activity cells. 2. The filter cartridge in the filtration and drainage mechanism can filter and collect cells with low activity in the culture medium, thereby completely separating them from cells with high activity. Subsequent operators only need to extract cells with high activity attached to the inner wall of the culture dish and multiple attachment plates. BRIEF DESCRIPTION OF THE DRAWINGS
[0014] Figure 1 This is a schematic diagram of the overall structure of a mesenchymal stem cell culture and separation device proposed in the present invention; Figure 2 This is a side sectional view of a mesenchymal stem cell culture and separation device proposed by the present invention; Figure 3 for Figure 2 Schematic diagram of the enlarged structure of part A; Figure 4 This is an exploded view of the suction mechanism in the mesenchymal stem cell culture and separation device proposed in the present invention; Figure 5 This is a schematic diagram of the overall structure of a mesenchymal stem cell culture and separation device proposed in the present invention, excluding the base; Figure 6 This is a top view of a culture dish and multiple attachment plates in a mesenchymal stem cell culture and separation device proposed by the present invention.
[0015] In the figure: 1. Petri dish; 2. Dish cover; 3. Base; 4. Suction mechanism; 41. Annular plate; 42. Arc blade; 43. Circular plate; 5. Filtration and drainage mechanism; 51. Cylinder; 52. Annular box 1; 53. Liquid outlet; 54. Filter cartridge; 55. Conical cylinder; 56. Annular box 2; 57. Guide rod; 58. Spring; 59. Pressing ring; 510. Motor; 511. Transmission shaft; 512. Liquid outlet; 513. Drain pipe; 6. Attachment plate; 61. Circular shaft; 7. Synchronizing mechanism; 71. Gear; 72. Ring gear; 73. Cylinder; 74. Rotating shaft 1; 75. Rotating shaft 2; 76. Tension spring; 8. Filling pipe; 9. Drain valve. DETAILED DESCRIPTION
[0016] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.
[0017] Please refer to Figures 1-6 The present invention provides a technical solution: a mesenchymal stem cell culture and separation device, comprising a culture dish 1, a dish cover 2 detachably mounted on the top of the culture dish 1, a filtering and draining mechanism 5 mounted on the dish cover 2, a suction mechanism 4 rotatably mounted inside the culture dish 1, and a plurality of attachment plates 6 rotatably mounted on the inner wall of the bottom of the culture dish 1 distributed in a circular array, the filtering and draining mechanism 5 is used to drive the suction mechanism 4 to form a vortex in the culture fluid inside the culture dish 1, so that the culture fluid in the culture dish 1 can be evenly distributed through the vortex, and the vortex can flush out cells with low activity on the inner wall of the culture dish 1 and the plurality of attachment plates 6, and the flushed cells with low activity are collected by the filtering and draining mechanism 5, a drain valve 9 is provided through the culture dish 1, and the filtering and draining mechanism 5 and the drain valve 9 cooperate to drain the old culture fluid in the culture dish 1, a filling pipe 8 is fixedly mounted through the dish cover 2, a base 3 is fixedly mounted on the bottom of the culture dish 1, and a synchronization mechanism 7 for causing the plurality of attachment plates 6 to move synchronously is installed in the base 3.
[0018] The filtering and drainage mechanism 5 includes a motor 510 fixedly mounted on the top of the dish cover 2. The motor 510 rotates the output shaft to rotate through the dish cover 2, and a transmission shaft 511 is fixedly mounted on the end of the output shaft of the motor 510. The bottom end of the transmission shaft 511 is detachably mounted with an annular box 52, and the bottom of the annular box 52 is detachably mounted with a cylinder 51. The suction mechanism 4 includes an arc-shaped blade 42 whose bottom is rotatably connected to the inner wall of the bottom of the culture dish 1. A plurality of circular plates 43 distributed in a circular array are fixedly mounted on the top of the plurality of circular plates 43. The same annular plate 41 is fixedly mounted on the top of the plurality of circular plates 43, and the annular plate 41 is fixedly sleeved on the cylinder 51.
[0019] The top of the cylinder 51 is flush with the bottom inner wall of the annular box 52. A plurality of liquid outlets 53 distributed in a circular array are provided on the inner wall of the annular box 52. A filter cartridge 54 is detachably mounted on the inner wall of the annular box 52.
[0020] A conical cylinder 55 is fixedly mounted on the top end of the cylinder 51 .
[0021] Furthermore, the liquid level of the culture solution added to the culture dish 1 is not allowed to be higher than the conical cylinder 55, so as to ensure that after the suction mechanism 4 stops rotating, the cells with low activity filtered out in the annular box 1 52 will not re-enter the cylinder 51.
[0022] The filtering and draining mechanism 5 also includes a plurality of guide rods 57 that pass through the dish cover 2 and are slidably connected to the dish cover 2. The plurality of guide rods 57 are distributed in a circular array. The bottom ends of the plurality of guide rods 57 are fixedly mounted with the same annular box 2 56. The annular box 2 56 is coaxially arranged with the annular box 1 52, and the inner wall of the annular box 2 56 is open. The inner diameter of the annular box 2 56 is equal to the outer diameter of the annular box 1 52. The top ends of the plurality of guide rods 57 are fixedly mounted with the same pressure ring 59. A spring 58 is provided on the sliding sleeve of the guide rod 57. The two ends of the spring 58 are respectively fixedly connected to the annular box 2 56 and the dish cover 2.
[0023] The filtering and draining mechanism 5 also includes a drain pipe 513 fixedly connected to the inner wall of the drain valve 9. The drain pipe 513 is L-shaped. A liquid outlet hole 512 is provided on the inner wall of the bottom of the annular box 56. The liquid outlet hole 512 is coaxially arranged with the end of the drain pipe 513 away from the drain valve 9, and the inner diameter of the liquid outlet hole 512 is equal to the outer diameter of the drain pipe 513.
[0024] A circular shaft 61 is fixedly installed on the attachment plate 6, which passes through the bottom of the culture dish 1 and is rotatably connected to the culture dish 1. The bottom end of the circular shaft 61 extends into the base 3. The synchronization mechanism 7 includes multiple gears 71 fixedly installed on the bottom ends of multiple circular shafts 61. The multiple gears 71 are meshed with the same ring gear 72. The synchronization mechanism 7 also includes a cylinder 73 fixedly installed on the bottom of the culture dish 1. The cylinder 73 is coaxially arranged with the culture dish 1, and the ring gear 72 is rotatably sleeved on the cylinder 73.
[0025] The bottom of the gear ring 72 is rotatably mounted with a plurality of rotating shafts 74 distributed in a circular array, and the bottom of the culture dish 1 is rotatably mounted with a plurality of rotating shafts 75 distributed in a circular array. The number of rotating shafts 1 74 and rotating shafts 2 75 is equal, and the adjacent rotating shafts 2 75 and rotating shafts 1 74 are fixedly mounted with the same tension spring 76.
[0026] Furthermore, when the filtration and drainage mechanism 5 drives the suction mechanism 4 to rotate, the culture liquid inside the culture dish 1 will form a vortex, and through the flushing of the vortex, the multiple attachment plates 6 will be deflected. At this time, the shapes of the multiple attachment plates 6 are as follows: Figure 6 As shown, at this time, the vortex can flush the inner wall of the culture dish 1 and both sides of the multiple attachment plates 6, thereby ensuring that the vortex can flush down the cells with low activity attached to the inner wall of the culture dish 1 and the multiple attachment plates 6; When the attachment plate 6 is about to deflect, it will drive the circular shaft 61 to rotate, and the rotating circular shaft 61 will drive the gear 71 to rotate. Through the connection of the ring gear 72, the multiple gears 71 and the multiple circular shafts 61 will rotate synchronously at the same angle, ultimately ensuring that no matter what speed the suction mechanism 4 is at, the flushing force on both sides of the multiple attachment plates 6 will not be too different. When the ring gear 72 rotates, it will stretch the tension spring 76 through the multiple rotating shafts 74. When the suction mechanism 4 stops rotating, the stretched multiple tension springs 76 will drive the ring gear 72 and the multiple attachment plates 6 to reset.
[0027] In this embodiment, when in use, the culture medium is added into the culture dish 1 through the opened filling tube 8. During the culture process, cells with higher activity will attach to the inner wall of the culture dish 1 and the multiple attachment plates 6. Dead cells and most of the cells with lower activity will be suspended in the culture medium or accumulate at the bottom of the culture dish 1. A small number of cells with lower activity will also attach to the inner wall of the culture dish 1 and the multiple attachment plates 6. After a period of time, the motor 510 is started, and the motor 510 rotates and drives the annular box 1 52 through the transmission shaft 511. The annular box 1 52 then drives the cylinder 51 to rotate, and the cylinder 51 then drives the suction mechanism 4 to rotate. During the rotation of the suction mechanism 4, the culture fluid will flow from the gaps between the multiple circular plates 43 to the center of the annular plate 41 and be transported into the cylinder 51. In addition, during the rotation of the suction mechanism 4, the culture fluid in the culture dish 1 will form a vortex. The culture fluid forming the vortex continuously flushes the inner wall of the culture dish 1 and the multiple attachment plates 6, and can flush out the low-activity cells attached to the inner wall of the culture dish 1 and the attachment plates 6. During the continuous rotation of the suction mechanism 4, the culture fluid entering the cylinder 51 will continuously surge up and pass through the conical cylinder 55 into the annular box 1 52. After being filtered by the filter cylinder 54, the low-activity cells in the culture fluid are filtered out by the filter cylinder 54 and accumulated in the annular box 1 52. The filtered culture fluid flows out through the multiple liquid outlets 53 and falls back into the culture dish 1. When the culture medium needs to be replaced, the drain valve 9 is first opened, and then the pressure ring 59 is pressed downward. The pressure ring 59 overcomes the elastic force of the multiple springs 58 and drives the annular box 2 56 downward through the multiple guide rods 57. When the bottom of the pressure ring 59 is in contact with the top of the dish cover 2, the top and bottom of the annular box 2 56 will be flush with the bottom of the annular box 1 52. At this time, the annular box 1 52 and the annular box 2 56 are in a rotational connection relationship. At the same time, the drain pipe 513 will be inserted into the liquid outlet 512, and the end of the liquid outlet 512 will be flush with the inner wall of the bottom of the annular box 2 56. Then, under the continuous rotation of the suction mechanism 4, the culture medium flowing out of the multiple liquid outlets 53 will enter the annular box 2 56, and finally be discharged through the liquid outlet 512, the drain pipe 513 and the opened drain valve 9. When there is no culture medium discharged from the drain valve 9, the pressure ring 59 is released, the drain valve 9 is closed, and new culture medium is re-injected into the culture dish 1 through the opened filling pipe 8; When it is necessary to remove cells with higher activity, most of the culture fluid in the culture dish 1 is first discharged through the rotating suction mechanism 4. After this operation, it can be ensured that the cells attached to the inner wall of the culture dish 1 and the multiple attachment plates 6 are all highly active. Then the dish cover 2 is removed, and the remaining culture fluid is discharged by tilting the culture dish 1. The subsequent operator can then extract the cells with higher activity attached to the inner wall of the culture dish 1 and the multiple attachment plates 6.
[0028] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.
Claims
1. A mesenchymal stem cell culture and separation device, comprising a culture dish (1), characterized in that: The top of the culture dish (1) is detachably mounted with a dish cover (2), and a filtering and draining mechanism (5) is mounted on the dish cover (2). A suction mechanism (4) is rotatably mounted inside the culture dish (1), and a plurality of attachment plates (6) distributed in a circular array are rotatably mounted on the inner wall of the bottom of the culture dish (1). The filtering and draining mechanism (5) is used to drive the suction mechanism (4) to form a vortex in the culture liquid inside the culture dish (1), and the vortex can make the culture liquid in the culture dish (1) evenly distributed, and the inner wall of the culture dish (1) and the plurality of attachment plates (6) are rotatably mounted on the inner wall of the culture dish (1). The cells with lower activity on the plate (6) are washed down, and the washed down cells with lower activity are collected by the filtering and draining mechanism (5). A drain valve (9) is provided through the culture dish (1). The filtering and draining mechanism (5) and the drain valve (9) cooperate to discharge the old culture fluid in the culture dish (1). A filling pipe (8) is fixedly installed through the dish cover (2). A base (3) is fixedly installed at the bottom of the culture dish (1). A synchronization mechanism (7) for making multiple attachment plates (6) move synchronously is installed in the base (3).
2. The mesenchymal stem cell culture and separation device according to claim 1, characterized in that: The filtering and draining mechanism (5) includes a motor (510) fixedly mounted on the top of the culture dish (2), wherein the motor (510) rotates an output shaft that rotates through the culture dish (2), and a transmission shaft (511) is fixedly mounted on the end of the output shaft of the motor (510), and an annular box (52) is detachably mounted on the bottom end of the transmission shaft (511), and a cylinder (51) is detachably mounted through the bottom of the annular box (52), and the suction mechanism (4) includes an arc-shaped blade (42) whose bottom is rotatably connected to the inner wall of the bottom of the culture dish (1), and a plurality of circular plates (43) distributed in a circumferential array are fixedly mounted on the top of the plurality of circular plates (43), and the same annular plate (41) is fixedly mounted on the top of the plurality of circular plates (43), and the annular plate (41) is fixedly sleeved on the cylinder (51).
3. The mesenchymal stem cell culture and separation device according to claim 2, characterized in that: The top of the cylinder (51) is flush with the bottom inner wall of the annular box (52). The inner wall of the annular box (52) is provided with a plurality of liquid outlets (53) distributed in a circumferential array. A filter cartridge (54) is detachably mounted on the inner wall of the annular box (52).
4. The mesenchymal stem cell culture and separation device according to claim 2, characterized in that: A conical cylinder (55) is fixedly mounted on the top end of the cylinder (51).
5. The mesenchymal stem cell culture and separation device according to claim 2, characterized in that: The filtering and draining mechanism (5) further comprises a plurality of guide rods (57) penetrating the dish cover (2) and being slidably connected to the dish cover (2), wherein the plurality of guide rods (57) are distributed in a circular array, and the bottom ends of the plurality of guide rods (57) are fixedly mounted with the same annular box 2 (56), the annular box 2 (56) is coaxially arranged with the annular box 1 (52), and the inner wall of the annular box 2 (56) is open, and the inner diameter of the annular box 2 (56) is equal to the outer diameter of the annular box 1 (52), and the top ends of the plurality of guide rods (57) are fixedly mounted with the same pressure ring (59), and a spring (58) is provided on the sliding sleeve of the guide rod (57), and the two ends of the spring (58) are respectively fixedly connected with the annular box 2 (56) and the dish cover (2).
6. The mesenchymal stem cell culture and separation device according to claim 5, characterized in that: The filtering and draining mechanism (5) further comprises a drain pipe (513) fixedly connected to the inner wall of the drain valve (9), wherein the drain pipe (513) is L-shaped, and a liquid outlet hole (512) is provided on the inner wall of the bottom of the annular box (56), wherein the liquid outlet hole (512) is coaxially arranged with an end of the drain pipe (513) away from the drain valve (9), and the inner diameter of the liquid outlet hole (512) is equal to the outer diameter of the drain pipe (513).
7. The mesenchymal stem cell culture and separation device according to claim 1, characterized in that: A circular shaft (61) is fixedly installed on the attachment plate (6), and the circular shaft (61) passes through the bottom of the culture dish (1) and is rotatably connected to the culture dish (1). The bottom end of the circular shaft (61) extends into the base (3). The synchronization mechanism (7) includes a plurality of gears (71) fixedly installed at the bottom ends of the plurality of circular shafts (61), and the plurality of gears (71) are meshed with a same gear ring (72). The synchronization mechanism (7) also includes a cylinder (73) fixedly installed at the bottom of the culture dish (1), the cylinder (73) is coaxially arranged with the culture dish (1), and the gear ring (72) is rotatably sleeved on the cylinder (73).
8. The mesenchymal stem cell culture and separation device according to claim 7, characterized in that: The bottom of the gear ring (72) is rotatably mounted with a plurality of rotating shafts (74) distributed in a circumferential array, and the bottom of the culture dish (1) is rotatably mounted with a plurality of rotating shafts (75) distributed in a circumferential array. The number of rotating shafts (74) and rotating shafts (75) is equal, and the adjacent rotating shafts (75) and rotating shafts (74) are fixedly mounted with the same tension spring (76).