Clostridium praeparatum culture device

Abstract

The invention discloses a clostridium praeparatum culture device, and relates to the technical field of microbial fermentation equipment, the clostridium praeparatum culture device comprises a tank body, a heating pipe is arranged on the outer wall of the tank body, a monitoring probe is arranged in the tank body, the monitoring probe is connected with the heating pipe, a working assembly is arranged in the tank body, a driving motor is arranged at the lower part of the working assembly, and an auxiliary assembly is arranged at the bottom in the tank body; a filling assembly is arranged at the top of the working assembly and connected with the working assembly, and an adding assembly is arranged in the tank body and arranged on the working assembly in a sleeving mode. Through the design of the working assembly, especially the combination of the working main shaft, the working rotating shaft, the working stirring fan blade and the reinforcing circular hoop, it is ensured that raw materials are fully mixed in the tank body, meanwhile, damage to the raw materials in the stirring process is avoided, and therefore the fermentation culture efficiency of clostridium praeparatum is improved. The arrangement of the filling assembly allows a catalyst to be added in real time in the fermentation culture process, and the substances can be uniformly distributed into a mixed solution through the dripping holes, so that the fermentation culture process is further promoted.

Description

Technical Field

[0001] The invention relates to the technical field of microbial fermentation equipment, in particular to a Clostridium prausnitzii culture device. Background Art

[0002] Faecalibacterium prausnitzii is an abundant microorganism in the healthy human intestine. It fights inflammation by producing substances such as butyrate. A reduction in its abundance may contribute to the development of conditions such as type 2 diabetes and cardiovascular disease. However, Faecalibacterium prausnitzii is extremely sensitive to oxygen and can only survive stably in an anaerobic environment, which poses challenges to its culture, expansion, and fermentation.

[0003] The amplification and fermentation of Faecalibacterium prausnitzii requires a relatively strict environment, but there is no special fermentation device for Faecalibacterium prausnitzii in the existing technology, and the normal amplification and fermentation of Faecalibacterium prausnitzii cannot be guaranteed.

[0004] Therefore, it is necessary to propose a Clostridium prausnitzii culture device to solve the above problems. Summary of the Invention

[0005] The object of the present invention is to provide a Clostridium prausnitzii culture device to solve the problems existing in the prior art.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] A Clostridium prausnitzii culture device includes a tank body, a heating tube is provided on the outer wall of the tank body, a monitoring probe is provided inside the tank body, the monitoring probe is connected to the heating tube, a working component is provided inside the tank body, a driving motor is provided at the bottom of the working component, an auxiliary component is provided at the bottom of the tank body, a filling component is provided on the top of the working component, the filling component is connected to the working component, and an adding component is provided inside the tank body, and the adding component is sleeved on the working component.

[0008] As described above, a Clostridium prausnitzii culture device, the working component includes a working spindle, the bottom end of the working spindle is connected to the driving motor through a connecting transmission shaft, the top end of the working spindle is connected to the filling component, the working spindle is provided with a working shaft, the working spindle is provided with a working stirring blade, the top end of the working stirring blade is provided with a reinforcing circular hoop, the working spindle is in a hollow state, and the working spindle is provided with a filling hole.

[0009] In the above-mentioned Clostridium prausnitzii culture device, the filling hole is arranged obliquely on the working main shaft.

[0010] As described above, a Clostridium prausnitzii culture device, the auxiliary component includes an auxiliary tube, the auxiliary tube is provided with a fixed base, the fixed base is provided with a fixed column, the fixed column is fixed on the inner wall of the tank body, the auxiliary tube is provided with an auxiliary hole, the auxiliary tube is spirally arranged inside the tank body, one end of the auxiliary tube is arranged outside the tank body, one end of the auxiliary tube is provided with an auxiliary connecting flange, and a one-way valve is provided in the auxiliary tube.

[0011] As described above, the Clostridium prausnitzii culture device, the adding component includes an adding main pipe, the adding main pipe is connected to the working main shaft, a buckle cover is provided at the connection between the adding main pipe and the working main shaft, the buckle cover is set on the tank body, and a one-way valve is provided in the adding main pipe.

[0012] In the Clostridium prausnitzii culture device as described above, a support rod is provided at the upper portion of the connection between the working main shaft and the connecting transmission shaft, and the support rod is arranged inside the tank body.

[0013] In the above-mentioned Clostridium prausnitzii culture device, the tank body is provided with a support seat and a lifting lug.

[0014] In the above-mentioned Clostridium prausnitzii culture device, the driving motor is fixed on a mounting base plate, and the mounting base plate is arranged on the bottom surface of the working environment.

[0015] As described above, a Clostridium prausnitzii culture device, the filling assembly includes a filling pipe, the filling pipe is arranged on the inner wall of the tank through a fixing buckle, the bottom end of the filling pipe is arranged outside the tank, the top end of the filling pipe is provided with a filling circular tube, the filling circular tube is connected to the filling pipe, the filling circular tube is fixed to the inner wall of the tank through a fixing buckle, a drip hole is provided on the filling circular tube, and a one-way valve is provided on the filling pipe.

[0016] In the above-mentioned Clostridium prausnitzii culture device, the drip hole is obliquely arranged on the filling circular tube.

[0017] In the above-mentioned Clostridium prausnitzii culture device, a discharge pipe is provided at the bottom of the tank body, the discharge pipe is connected to the tank body, a sealing cover is provided on the discharge pipe, and a handle is provided on the sealing cover.

[0018] In the above-mentioned Clostridium prausnitzii culture device, the inner wall of the tank is coated with an anti-corrosion coating.

[0019] The advantages of the present invention are as follows: 1. The design of the working components, particularly the combination of the working spindle, working shaft, working stirring blades, and reinforced circular hoop, ensures thorough mixing of the raw materials within the tank while preventing damage to the raw materials during the stirring process, thereby improving the fermentation efficiency of Faecalibacterium prausnitzii. 2. The provision of the filling component allows for the real-time addition of catalysts or other necessary substances during the fermentation process. These substances can be evenly distributed into the mixed liquid through the dripping hole, further promoting the fermentation process.

[0020] 2. The real-time monitoring function of the monitoring probe ensures that the tank environment is always oxygen-free, which is a key condition for the normal expansion and fermentation of Faecalibacterium prausnitzii. The auxiliary component design allows the injection of nitrogen or other inert gases into the tank when necessary to further reduce the oxygen content and thus optimize the fermentation environment.

[0021] 3. The monitoring data is transmitted wirelessly to the external data terminal, and the start and shut down of the drive motor, heating tube and other equipment can be remotely controlled, which improves the intelligence and automation level of the entire system. This design makes the operation easier and also improves the response speed and accuracy of the system.

[0022] 4. The anti-corrosion coating applied to the inner wall of the tank extends the service life of the equipment and reduces maintenance costs. All parts that come into contact with raw materials have been treated with anti-corrosion treatment, further ensuring the long-term stable operation of the equipment.

[0023] 5. The support base and lifting lugs on the tank body provide a convenient way to move and adjust the position, so that the equipment can adapt to different working environments and layout requirements. The design of the discharge pipe allows for easy discharge of the product after the fermentation is completed, and also provides convenience for cleaning and maintenance.

[0024] 6. The sealing cover and one-way valve ensure the sealing and safety of the tank, preventing gas leakage and the entry of external pollutants. The reinforced hoop design also improves the stability of the equipment during the mixing process, preventing safety hazards caused by the falling of the mixing blades. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following is a brief introduction to the drawings required for use in the description of the embodiments. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0026] Figure 1 It is a structural schematic diagram of the present invention;

[0027] Figure 2 is a first stereogram of the present invention;

[0028] Figure 3 is a second perspective view of the present invention;

[0029] Figure 4 is a third perspective view of the present invention;

[0030] Figure 5 This is the fourth stereogram of the present invention

[0031] Figure 6 It is the front view of the present invention.

[0032] Figure markings: 1. Tank body; 2. Heating tube; 3. Monitoring probe; 4. Working assembly; 5. Driving motor; 6. Auxiliary assembly; 7. Filling assembly; 8. Adding assembly; 11. Support seat; 12. Lifting ear; 13. Discharge pipe; 14. Sealing cover; 15. Handle; 41. Working spindle; 42. Connecting drive shaft; 43. Working rotating shaft; 44. Working stirring blade; 45. Reinforcement hoop; 46. Filling hole; 47. Support rod; 51. Mounting base; 61. Auxiliary pipe; 62. Fixed base; 63. Fixed column; 64. Auxiliary hole; 65. Connecting flange; 66. One-way valve; 71. Filling pipe; 72. Fixing buckle; 73. Filling round pipe; 74. Drip hole; 81. Adding main pipe; 82. Buckle cover. DETAILED DESCRIPTION

[0033] To make the objectives, technical solutions, and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings of the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. All other embodiments obtained by ordinary technicians in this field based on the embodiments of the present invention without making any creative efforts shall fall within the scope of protection of the present invention.

[0034] like Figures 1-6As shown, a Clostridium prausnitzii culture device includes a tank body 1, a heating tube 2 is provided on the outer wall of the tank body 1, a monitoring probe 3 is provided inside the tank body 1, the monitoring probe 3 is connected to the heating tube 2, a working component 4 is provided inside the tank body 1, a driving motor 5 is provided at the lower part of the working component 4, an auxiliary component 6 is provided at the bottom of the tank body 1, a filling component 7 is provided on the top of the working component 4, the filling component 7 is connected to the working component 4, an adding component 8 is provided inside the tank body 1, and the adding component 8 is sleeved on the working component 4. When using the present invention, the components need to be connected together according to their connection relationship. The user adds various raw materials to the interior of the tank body 1 through the adding component 8, and the working component 4 stirs and mixes the various raw materials. In the present invention, the components that directly contact the various raw materials are all subjected to anti-corrosion treatment, which can extend the service life of the present invention. At the same time, the protective layer generated by the anti-corrosion treatment will not affect the normal expansion and fermentation of Clostridium prausnitzii. After the various raw materials enter the interior of the tank body 1, the monitoring probe 3 monitors the environment inside the tank body 1 in real time to ensure that the interior of the tank body 1 is an oxygen-free environment. Nitrogen or other gases can be injected into the interior of the tank body 1 through the auxiliary component 6 to ensure the normal expansion and fermentation of Clostridium prausnitzii. The working component 4 stirs and mixes the various raw materials. At the same time, the stirring and mixing speed of the working component 4 is relatively slow, and the various raw materials will not adhere to the working component 4, and the working component 4 will not damage the various raw materials. It only plays a role in stirring and mixing, which can promote the full fermentation of the raw materials. When a catalyst needs to be added, the catalyst can be added to the interior of the tank body 1 through the filling component 7. Since the top of the filling component 7 is set at the top of the tank body 1, the catalyst can be fully mixed with the raw materials, increasing the expansion and fermentation efficiency of Clostridium prausnitzii, and further increasing the use efficiency of the present invention.

[0035] Specifically, if Figures 1-6As shown, the working assembly 4 described in this embodiment includes a working spindle 41. The bottom end of the working spindle 41 is connected to the drive motor 5 through a connecting transmission shaft 42. The top end of the working spindle 41 is connected to the filling assembly 7. A working rotating shaft 43 is provided on the working rotating shaft 43. A working stirring blade 44 is provided on the working stirring blade 44. A reinforcing hoop 45 is provided on the top end of the working spindle 41. The working spindle 41 is hollowed out and a filling hole 46 is provided on the working spindle 41. The filling hole 46 is tilted on the working spindle 41. The drive motor 5 is fixed on a mounting base 51, and the mounting base 51 is set on the bottom surface of the working environment. A support rod 47 is provided on the upper part of the connection between the working spindle 41 and the connecting transmission shaft 42, and the support rod 47 is set inside the tank body 1. The filling assembly 7 includes a filling pipe 71, which is mounted on the inner wall of the tank body 1 via a fixing buckle 72. The bottom end of the filling pipe 71 is mounted on the outside of the tank body 1. The top end of the filling pipe 71 is provided with a filling circular pipe 73, which is connected to the filling pipe 71 and fixed to the inner wall of the tank body 1 via the fixing buckle 72. The filling circular pipe 73 is provided with a drip hole 74, and the filling pipe 71 is provided with a one-way valve 66. The drip hole 74 is arranged obliquely on the filling circular pipe 73. The adding assembly 8 includes an adding main pipe 81, which is connected to the working spindle 41. A buckle cover 82 is provided at the connection between the adding main pipe 81 and the working spindle 41. The buckle cover 82 is mounted on the tank body 1, and the adding main pipe 81 is provided with a one-way valve 66.The user first mixes the various raw materials, and then uses the filling equipment to add the various raw materials into the tank body 1 through the adding main pipe 81. The buckle cover 82 ensures the sealing of the connection between the adding main pipe 81 and the tank body 1, and ensures the sealing of the tank body 1. After the various raw materials enter the tank body 1, the monitoring probe 3 monitors the environment in the tank body 1 in real time, and can also monitor the pH value of the mixed solution of various raw materials to ensure the fermentation and expansion of Clostridium prausnitzii. The data monitored in real time by the monitoring probe 3 can be transmitted to an external data terminal in a wireless manner. The data terminal analyzes the data, and then the external control switch can control the start and stop of the drive motor 5, and can also adjust the start and stop of the heating tube 2, which can assist in adjusting the temperature or pressure inside the tank body 1 to meet the fermentation environment of Clostridium prausnitzii. The drive motor 5 starts and drives the working spindle 41 to rotate, so that the residue left in the working spindle 41 can be removed. All the raw materials are thrown out, and the raw materials can be added to the inside of the tank body 1 in real time. In this way, it can rotate with the working main shaft 41 and cooperate with the working stirring blades 44 to fully mix the various raw materials and increase the fermentation efficiency of Clostridium prausnitzii. Because the working stirring blades 44 are provided with a reinforcing hoop 45, the working stirring blades 44 will not damage the raw materials and will not affect the fermentation of Clostridium prausnitzii, thereby ensuring the normal use of the present invention. The staff can judge whether the catalyst needs to be added by monitoring the data transmitted by the probe 3. When the catalyst needs to be added to the inside of the tank body 1, the external filling equipment is used to add the catalyst to the inside of the tank body 1 through the filling pipe 71. Because the filling pipe 73 is mounted on the working main shaft 41 and fixed on the inner wall of the top of the tank body 1, the drip hole 74 can drip the catalyst. As the working stirring blades 44 rotate, the catalyst and the raw materials can be fully mixed to promote the full fermentation of Clostridium prausnitzii.

[0036] Specifically, such as Figures 1-6 As shown, the auxiliary assembly 6 of this embodiment includes an auxiliary tube 61, which is provided with a fixing base 62, which is provided with a fixing column 63. The fixing column 63 is fixed to the inner wall of the tank body 1, and the auxiliary tube 61 is provided with an auxiliary hole 64. The auxiliary tube 61 is spirally arranged inside the tank body 1, and one end of the auxiliary tube 61 is arranged outside the tank body 1. One end of the auxiliary tube 61 is provided with an auxiliary connecting flange 65, and a one-way valve 66 is provided inside the auxiliary tube 61. When the monitoring probe 3 detects that the oxygen content in the tank body 1 is too high, because Faecalibacterium prausnitzii is anaerobic, the oxygen content in the tank body 1 needs to be reduced. Nitrogen or other gases need to be added to the tank body 1 through the auxiliary tube 61 through an external adding device to reduce the oxygen concentration to meet the normal expansion and fermentation of Faecalibacterium prausnitzii. The function of the one-way valve 66 is to prevent gas backflow and other gases from entering the tank body 1, which would affect the normal fermentation and expansion of Faecalibacterium prausnitzii.

[0037] Further, such as Figures 1-6As shown, the tank body 1 described in this embodiment is provided with a support seat 11 and a lifting lug 12. A discharge pipe 13 is provided at the bottom of the tank body 1, and the discharge pipe 13 is connected to the tank body 1. A sealing cover 14 is provided on the discharge pipe 13, and a handle 15 is provided on the sealing cover 14. The inner wall of the tank body 1 is coated with an anti-corrosion coating. In this embodiment, after the monitoring probe 3 monitors the fermentation and amplification of Clostridium prausnitzii, it needs to be discharged through the discharge port. When the tank body 1 is working normally, it is in a sealed state to ensure the sealing inside the tank body 1. The support seat 11 is convenient for fixing the tank body 1. The lifting lug 12 is used in conjunction with the support seat 11. The present invention needs to be lifted by using the lifting lug 12 to adjust the working position. The support seat 11 can place the tank body 1 to crush the drive motor 5. The anti-corrosion coating extends the service life of the tank body 1 and further extends the service life of the present invention.

[0038] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the various embodiments of the present invention.

Claims

1. A Clostridium prausnitzii culture device, characterized in that: The invention comprises a tank body (1), a heating tube (2) is provided on the outer wall of the tank body (1), a monitoring probe (3) is provided inside the tank body (1), the monitoring probe (3) is connected to the heating tube (2), a working component (4) is provided inside the tank body (1), a driving motor (5) is provided at the lower part of the working component (4), an auxiliary component (6) is provided at the bottom of the tank body (1), a filling component (7) is provided at the top of the working component (4), the filling component (7) is connected to the working component (4), an adding component (8) is provided inside the tank body (1), and the adding component (8) is sleeved on the working component (4); The working assembly (4) includes a working spindle (41), the bottom end of the working spindle (41) is connected to the driving motor (5) through a connecting transmission shaft (42), the top end of the working spindle (41) is connected to the filling assembly (7), the working spindle (41) is provided with a working shaft (43), the working spindle (43) is provided with a working stirring blade (44), the top end of the working stirring blade (44) is provided with a reinforcing hoop (45), the working spindle (41) is in a hollow state, and the working spindle (41) is provided with a filling hole (46); The filling hole (46) is arranged obliquely on the working spindle (41); The auxiliary assembly (6) comprises an auxiliary pipe (61), a fixing base (62) is provided on the auxiliary pipe (61), a fixing column (63) is provided on the fixing base (62), the fixing column (63) is fixed on the inner wall of the tank body (1), an auxiliary hole (64) is provided on the auxiliary pipe (61), the auxiliary pipe (61) is spirally arranged inside the tank body (1), one end of the auxiliary pipe (61) is arranged outside the tank body (1), an auxiliary connecting flange (65) is provided on one end of the auxiliary pipe (61), and a one-way valve (66) is provided inside the auxiliary pipe (61); The adding assembly (8) comprises an adding main pipe (81), the adding main pipe (81) is connected to the working spindle (41), a buckle cover (82) is provided at the connection between the adding main pipe (81) and the working spindle (41), the buckle cover (82) is arranged on the tank body (1), and a one-way valve (66) is provided in the adding main pipe (81).

2. A Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: A support rod (47) is provided at the upper portion of the connection between the working main shaft (41) and the connecting transmission shaft (42), and the support rod (47) is arranged inside the tank body (1).

3. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: The tank body (1) is provided with a support seat (11) and a lifting lug (12).

4. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: The driving motor (5) is fixed on a mounting base plate (51), and the mounting base plate (51) is arranged on the bottom surface of the working environment.

5. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: The filling assembly (7) comprises a filling pipe (71), the filling pipe (71) being arranged on the inner wall of the tank body (1) via a fixing buckle (72), the bottom end of the filling pipe (71) being arranged outside the tank body (1), the top end of the filling pipe (71) being provided with a filling circular pipe (73), the filling circular pipe (73) being connected to the filling pipe (71), the filling circular pipe (73) being fixed to the inner wall of the tank body (1) via the fixing buckle (72), the filling circular pipe (73) being provided with a drip hole (74), and the filling pipe (71) being provided with a one-way valve (66).

6. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: The drip hole (74) is obliquely arranged on the filling circular tube (73).

7. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: A discharge pipe (13) is provided at the bottom of the tank body (1), the discharge pipe (13) is connected to the tank body (1), a sealing cover (14) is provided on the discharge pipe (13), and a handle (15) is provided on the sealing cover (14).

8. The Faecalibacterium prausnitzii culture device according to claim 1, characterized in that: The inner wall of the tank body (1) is coated with an anti-corrosion coating.

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