Biotechnology culture device suitable for microorganisms

Abstract

The invention relates to the technical field of microorganism culture, in particular to a biotechnological culture device suitable for microorganisms, which comprises a tank body and a main shaft, the bottom of the main shaft extends into the tank body and is rotationally matched with the tank body; a driving mechanism for driving the main shaft to rotate is arranged at the top of the main shaft; a plurality of stirring modules for stirring materials are arranged in the tank body; the stirring module comprises a stirring rod, a guide sleeve and a supporting rod; one end of the supporting rod is fixedly connected with the lower end of the main shaft, the other end of the supporting rod is fixedly connected with the guide sleeve, a guide hole is formed in the guide sleeve, the stirring rod penetrates through the guide hole and is rotationally connected with the guide sleeve, and the stirring rod is fixedly connected with a gear and a plurality of stirring blades sequentially from top to bottom in the axial direction; the distance between the upper end of the stirring rod and the main shaft is smaller than that between the lower end of the stirring rod and the main shaft, a gear ring is fixedly connected to the middle of the inner wall of the tank body, and the gears are meshed with the gear ring. The stirring device is complete in structure, and the uniformity and comprehensiveness of material stirring can be improved.

Description

Technical Field

[0001] The present invention relates to the technical field of microbial cultivation, and in particular to a biotechnology cultivation device suitable for microorganisms. Background Art

[0002] Microorganisms are a large group of organisms, including bacteria, fungi, some small protozoa, microscopic algae, and viruses. They are tiny, diverse, and closely associated with humans. They encompass a wide range of beneficial and harmful species and are widely involved in food, medicine, industry, agriculture, environmental protection, and other fields. Microorganisms are a general term for all tiny organisms that are difficult to observe with the naked eye. Microbiology is a typical life science discipline. The study of the structure, function, and interaction of microorganisms with their environment involves a wide range of applications. The application of microorganisms has significantly improved the quality of human life. Microorganisms have a wide range of applications, generally used for pathogen, pest, and weed control, crop induction, or formulated products based on the active ingredients of microorganisms. These are generally isolated from nature but can also be improved through artificial strains such as induced mutagenesis, selection, or genetic modification.

[0003] In the existing technology, a motor is mainly used to drive the stirring shaft and the stirring blades to rotate, thereby stirring the materials (mainly nutrient solution and solid culture matrix). However, this method easily leads to uneven mixing of materials, resulting in insufficient nutrient supply to microorganisms and failure of microbial culture.

[0004] In summary, solving the problem of uneven material mixing in existing culture devices, which leads to microbial culture failure, has become a difficult problem that needs to be solved in the current field. Therefore, it is necessary to provide a biotechnology culture device for microorganisms that can improve material mixing uniformity and increase the success rate of microbial culture. Summary of the Invention

[0005] To solve the above problems, the present invention provides a biotechnology culture device suitable for microorganisms. Through the design of the stirring module, it can improve the comprehensiveness and uniformity of material stirring, thereby improving the uniformity of material mixing and distribution, optimizing the culture environment of microorganisms, and improving the success rate of microbial culture.

[0006] In order to achieve the above-mentioned purpose, the technical solution of the present invention is as follows: a biotechnology culture device suitable for microorganisms includes a tank body and a main shaft, the top of the tank body is provided with a feed port and an infusion port, and the bottom of the tank body is provided with a discharge port; the bottom of the main shaft extends into the tank body and rotates with the tank body; the top of the main shaft is provided with a driving mechanism for driving it to rotate; and the tank body is provided with a plurality of stirring modules for stirring materials.

[0007] The stirring module includes a stirring rod, a guide sleeve and a support rod; one end of the support rod is fixedly connected to the lower end of the main shaft, and the other end of the support rod is fixedly connected to the guide sleeve. A guide hole is opened on the guide sleeve, and the stirring rod passes through the guide hole and is rotatably connected to the guide sleeve. The stirring rod is fixedly connected with a gear and several stirring blades in sequence from top to bottom along its axial direction.

[0008] The distance between the upper end of the stirring rod and the main shaft is smaller than the distance between the lower end of the stirring rod and the main shaft. A gear ring is fixedly connected to the middle of the inner wall of the tank body, and all gears are engaged with the gear ring; a gas supply mechanism for conveying gas into the tank body is installed on the top of the tank body.

[0009] The technical principles of the above solution are as follows: Solid culture medium and nutrient solution are input into the tank body through the feed port and the infusion port, and the main shaft is rotated by the driving mechanism, and the main shaft drives the support rod, the guide sleeve and the stirring rod to rotate around the main shaft axis; at the same time, the stirring rod drives the gear to move on the gear ring, and since the gear is engaged with the gear ring, the gear will rotate along the gear ring, thereby driving the stirring rod to rotate, and the stirring rod will drive the stirring blade to rotate; since the distance between the upper end of the stirring rod and the main shaft is smaller than the distance between the lower end of the stirring rod and the main shaft, the stirring shaft is inclined when rotating.

[0010] The above scheme has the following beneficial effects: 1. In the prior art, a mixing box is mainly used to load materials, and a motor is used to drive the mixing shaft and the mixing blades to rotate, thereby stirring the materials. This method can often only stir the materials in the middle of the mixing box, resulting in insufficient comprehensiveness and uniformity of the stirring, making the materials unevenly mixed, and seriously affecting the culture effect of microorganisms. The present invention uses the design of the support rod and the guide sleeve, so that the main shaft can drive several groups of stirring rods to revolve together with the main shaft as the axis, greatly expanding the range of stirring, so that the materials can be evenly mixed, thereby providing a good environment for the subsequent culture of microorganisms and improving the success rate of microbial culture.

[0011] 2. The present invention uses the design of gears and gear rings to enable the stirring shaft to rotate at the same time as it revolves. The rotation of the stirring shaft can drive the stirring blades to rotate, thereby generating a stronger shear force, further improving the stirring effect on the material. The revolution and rotation are carried out simultaneously, which not only ensures the stirring range, but also ensures the stirring intensity. Compared with the stirring shaft that only rotates in the prior art, the present invention greatly improves the uniformity and comprehensiveness of material mixing.

[0012] 3. In the present invention, when the stirring rod and the stirring blade rotate, the nutrient solution, solid culture matrix and microorganisms near the stirring rod will be driven to rotate accordingly, thereby improving the uniformity of the nutrient solution, solid culture matrix and microorganisms in the local area; at the same time, when the stirring rod and the stirring blade revolve, the nutrient solution, solid culture matrix and microorganisms in the tank body will also be driven to flow, thereby improving the uniformity of the distribution of the nutrient solution, solid culture matrix and microorganisms, thereby improving the overall uniformity of the nutrient solution, solid culture matrix and microorganisms in the tank body.

[0013] Furthermore, a stirring ring is provided in the tank body, and a plurality of grinding grooves are opened on the top of the stirring ring. The bottom of the stirring rod extends to the bottom of the adjacent grinding groove. The bottom wall of the tank body is rotatably connected to a rotating sleeve, and the outer wall of the rotating sleeve is fixedly connected to a plurality of scrapers. The ends of the scrapers away from the rotating sleeve are fixedly connected to the stirring ring; the bottoms of the scrapers are all in contact with the bottom wall of the tank body.

[0014] Beneficial effect: When the scraper rotates, it can scrape the accumulated materials at the bottom of the tank, thereby improving the cleanliness of the tank and the utilization rate of the materials.

[0015] Furthermore, the scrapers are all arranged at an angle.

[0016] Beneficial effect: The inclined scraper can also play a stirring role, and when the inclined scraper rotates, it will push the material at the bottom of the tank upward to the stirring blades, so that the stirring effect of the material is further improved.

[0017] Furthermore, the bottoms of the scrapers are all tapered.

[0018] Beneficial effect: The conical design of the scraper bottom can increase the sharpness of the scraper bottom, thereby improving the scraping effect of the scraper.

[0019] Furthermore, the lower ends of the stirring rods are all ellipsoidal; and the bottoms of the grinding tanks are all provided with discharge holes.

[0020] Beneficial effect: During the stirring process, some materials will accumulate in the grinding tank. When the stirring rod is driven, the bottom of the stirring rod will grind and crush the materials in the grinding tank, while preventing the materials from clogging the grinding tank and affecting the rotation of the stirring rod, and reducing the particle size of the materials, thereby improving the stirring effect.

[0021] Furthermore, a piston cavity is fixedly connected to the bottom of the tank body, and a piston plate is vertically slidably fitted in the piston cavity. A number of piston air inlets are opened on the piston plate, and the piston air inlets are connected to an air inlet check valve. A number of piston air outlets are opened on the bottom wall of the tank body, and the piston air outlets are connected to an air outlet check valve. A sliding column is fixedly connected to the center of the piston plate and is slidably connected to the bottom surface of the tank body. An L-shaped connecting rod is fixedly connected to the side of the sliding column, and a cylindrical pin is fixedly connected to the upper part of the connecting rod. A cylindrical groove wheel is coaxially provided at the lower end of the main shaft, and an annular groove for the sliding of the cylindrical pin is opened on the side wall of the groove wheel; the sliding column and the rotating sleeve are vertically slidably fitted.

[0022] Beneficial effect: The gas supply mechanism on the top of the tank is used to transport gas to the microorganisms in the tank from top to bottom, and the movement of the piston plate is then used to transport gas to the microorganisms in the tank from bottom to top, thereby ensuring that the gas inside the tank is sufficient and evenly distributed, thereby optimizing the cultivation environment of microorganisms.

[0023] Furthermore, the axis of the sliding column and the axis of the main shaft are in one of a coincidence state and a parallel state.

[0024] Beneficial effect: Such a design can reduce the difficulty of processing and producing the device; the axis of the sliding column and the axis of the main shaft only need to be kept parallel, and then the size of the connecting rod and the cylindrical pin can be appropriately adjusted to ensure that the cylindrical pin can slide in the annular groove, thereby ensuring the normal operation of the device. There is no need to install the sliding column and the main shaft coaxially, thereby reducing the difficulty of processing and producing the device.

[0025] Furthermore, a bulk material cover is fixedly sleeved on the main shaft, which is located above the gear ring and below the feed port and the infusion port; the bulk material cover is conical, with a plurality of bulk material holes on the bulk material cover, and a plurality of raised ridges are fixedly connected to the top of the bulk material cover.

[0026] Beneficial Effects: After passing through the feed and infusion ports, the material flows to the top of the bulk cover and is dispersed through the bulk holes, resulting in even distribution throughout the tank. Simultaneously, the rotation of the main shaft drives the bulk cover and the ribs to rotate synchronously. Due to the conical shape of the bulk cover, the material flows along the edges of the ribs under the action of centrifugal force, toward the edge of the bulk cover, and then into the tank, improving material utilization. The ribs not only guide the material but also serve as reinforcement ribs for the bulk cover, increasing its structural strength and service life.

[0027] Furthermore, it also includes a plurality of material turning blades, which all pass through the lower part of the adjacent stirring rod and are fixedly connected thereto; the material turning blades are all X-shaped with the adjacent stirring rod.

[0028] Beneficial effect: When the stirring rod rotates, the turning blades will also revolve and rotate together with the stirring rod, thereby improving the comprehensiveness and uniformity of material stirring.

[0029] Furthermore, a number of legs are fixedly connected to the bottom of the tank body, a discharge pipe is connected to the bottom of the tank body, and a dust cover is hinged at the feed port; a protective frame is fixedly sleeved on the groove wheel to prevent the material from clogging the annular groove, and the top of the protective frame is conical; a number of stirring shafts are fixedly connected to the outer wall of the protective frame.

[0030] Beneficial effects: The legs can support the entire device and improve the overall stability of the device. After conveying the solid culture matrix, use the dust cover to close the feed port in time to reduce the chance of the tank being contaminated by external dust. After the microbial culture is completed, the microorganisms and materials in the tank can be taken out through the discharge pipe. The protective frame can isolate the material from the groove wheel to prevent the material from clogging the annular groove, thereby improving the overall feasibility of the device. The conical design of the top of the protective frame can guide the material so that the material flows to the bottom of the tank, avoiding the accumulation of material on the top of the protective frame and improving the utilization rate of the material. In this process, the stirring shaft can further improve the stirring effect of the material.

[0031] Additional aspects and advantages of the present invention will be set forth in part in the description which follows and, in part, will be obvious from the description which follows, or may be learned by practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The figure is an axonometric view of a biotechnology culture device for microorganisms according to the present invention.

[0033] Figure 2 This is a schematic diagram of the internal installation of a biotechnology culture device suitable for microorganisms according to the present invention.

[0034] Figure 3 It is a side axonometric view of the internal structure of the biotechnology culture device suitable for microorganisms of the present invention.

[0035] Figure 4 This is a top axonometric view of the internal structure of the biotechnology culture device for microorganisms of the present invention.

[0036] Figure 5 It is a front view of the biotechnology culture device suitable for microorganisms of the present invention.

[0037] Figure 6 It is a top view of the biotechnology culture device suitable for microorganisms of the present invention.

[0038] Figure 7 for Figure 6 Cross-sectional view along direction BB.

[0039] Figure 8 for Figure 2 side view.

[0040] Figure 9 for Figure 8Cross-sectional view along CC direction.

[0041] Figure 10 for Figure 8 Cross-sectional view along DD direction.

[0042] Figure 11 for Figure 2 Bottom view of .

[0043] Figure 12 for Figure 2 Axonometric view of the center gear ring.

[0044] Figure 13 for Figure 12 side view.

[0045] Figure 14 for Figure 12 Top view of .

[0046] Figure 15 for Figure 14 Cross-sectional view along the AA direction.

[0047] Figure 16 for Figure 7 Enlarged view of part A.

[0048] Figure 17 The figure is a side view of the protective frame of the biotechnology culture device for microorganisms according to the present invention.

[0049] The figure marks in the drawings of the specification include: 1. tank body; 11. discharge pipe; 12. ejector; 13. air supply port; 14. dust cover; 15. pressure gauge; 16. support foot; 17. discharge port; 18. thrust bearing; 19. wear-resistant sleeve; 2. main shaft; 21. driven pulley; 22. stirring rod; 23. stirring blade; 24. turning blade; 25. guide sleeve; 26. gear; 27. gear ring; 28. groove wheel; 29. ​​annular groove; 3. piston chamber; 30. cylindrical pin; 31. sliding column; 32. connecting rod; 33. rotating sleeve; 34. piston air inlet; 35. piston plate; 36. piston air outlet; 4. bulk material cover; 41. bulk material hole; 42. ridge; 5. stirring ring; 51. scraper; 52. grinding groove; 53. discharge hole; 6. protective frame; 61. stirring shaft. DETAILED DESCRIPTION

[0050] The following is further described in detail through specific implementation methods: Example 1:

[0051] like Figure 1 and Figure 6As shown, a biotechnology culture device suitable for microorganisms includes a tank body 1 and a main shaft 2. The top of the tank body 1 is provided with a feed port and an infusion port (not shown in the figure), and the bottom of the tank body 1 is provided with a discharge port 17; the bottom of the main shaft 2 extends into the tank body 1 and rotates with the tank body 1; the top of the main shaft 2 is provided with a driving mechanism for driving the main shaft 2 to rotate; and the tank body 1 is provided with a plurality of stirring modules for stirring materials.

[0052] like Figure 2 、 Figure 3 and Figure 4 As shown, the stirring module includes a stirring rod 22, a guide sleeve 25 and a support rod; one end of the support rod is fixedly connected to the lower end of the main shaft 2 with a bolt, and the other end of the support rod is welded to the guide sleeve 25. A guide hole is opened on the guide sleeve 25, and the stirring rod 22 passes through the guide hole and is rotatably connected to the guide sleeve 25. The stirring rod 22 is fixedly connected with a gear 26 and a plurality of stirring blades 23 by bolts in sequence from top to bottom along its axial direction.

[0053] like Figure 5 As shown, the distance between the upper end of the stirring rod 22 and the main shaft 2 is smaller than the distance between the lower end of the stirring rod 22 and the main shaft 2, and a gear ring 27 is fixedly connected with bolts in the middle of the inner wall of the tank body 1, and the gears 26 are all engaged with the gear ring 27; an air supply mechanism for conveying gas into the tank body 1 is installed on the top of the tank body 1. In this embodiment, the air supply mechanism uses an ejector 12 in the prior art, and an air supply port 13 is opened on the ejector 12. The ejector 12 conveys external gas into the tank body 1 through the air supply port 13.

[0054] Among them, the shape and structure of the gear ring 27 are as follows Figure 12 、 Figure 13 、 Figure 14 and Figure 15 shown.

[0055] In this embodiment, the drive mechanism utilizes a conventional reduction motor (installed in an external environment, such as a conventional motor rack). The reduction motor output shaft is bolted to a primary pulley, and the top of the main shaft 2 is bolted to a driven pulley 21. The same belt is tensioned on both the primary and driven pulleys 21. When the operator activates the reduction motor, the primary pulley rotates the belt and the driven pulley 21, which in turn rotates the main shaft 2. A bearing is provided at the rotational connection between the main shaft 2 and the tank body 1, with an interference fit. In this embodiment, the bearing is a thrust bearing 18, ensuring stable rotation of the main shaft 2 within the tank body 1.

[0056] Specifically, the operator inputs solid culture medium and nutrient solution into tank body 1 through the feed port and infusion port, starts the reduction motor, and rotates main shaft 2, which in turn drives the support rod, guide sleeve 25, and stirring rod 22 to rotate around the axis of main shaft 2. At the same time, stirring rod 22 drives gear 26 to move on gear ring 27. Since gear 26 meshes with gear ring 27, gear 26 rotates along gear ring 27, which in turn drives stirring rod 22 to rotate, and stirring rod 22 drives stirring blade 23 to rotate. Through the revolution and rotation of stirring rod 22, the device can comprehensively and evenly stir the material.

[0057] like Figure 7 、 Figure 8 、 Figure 9 and Figure 10 As shown, a stirring ring 5 is provided in the tank body 1, and a plurality of grinding grooves 52 are opened on the top of the stirring ring 5. The bottom of the stirring rod 22 extends to the bottom of the adjacent grinding groove 52. The inner bottom wall of the tank body 1 is rotatably connected to a rotating sleeve 33. A plurality of scrapers 51 are welded to the outer wall of the rotating sleeve 33. The ends of the scrapers 51 away from the rotating sleeve 33 are welded to the stirring ring 5; the bottoms of the scrapers 51 are in contact with the inner bottom wall of the tank body 1; the scrapers 51 are arranged at an angle, and the bottoms of the scrapers 51 are conical.

[0058] Specifically, due to the limitation of the grinding groove 52 on the stirring rod 22, when the stirring rod 22 rotates around the main shaft 2, it will drive the stirring ring 5 to rotate around the rotating sleeve 33, and at the same time drive the scraper 51 and the rotating sleeve 33 to rotate. When the scraper 51 rotates, it can scrape the accumulated and attached materials at the bottom of the tank body 1, thereby improving the cleanliness of the tank body 1 and the utilization rate of the materials; the conical design of the bottom of the scraper 51 can increase the sharpness of the bottom of the scraper 51, thereby improving the scraping effect of the scraper 51. In this process, the inclined scraper 51 can also play a stirring role, and when the inclined scraper 51 rotates, it will push the materials at the bottom of the tank body 1 upward to the stirring blade 23, so that the stirring effect of the materials is further improved.

[0059] like Figure 7 and Figure 16 As shown, the lower end of the stirring rod 22 is ellipsoidal, and the bottom of the grinding groove 52 is provided with a discharge hole 53.

[0060] like Figure 7As shown, a piston cavity 3 is integrally formed below the tank body 1, and a piston plate 35 is vertically slidably fitted in the piston cavity 3. A plurality of piston air inlets 34 are provided on the piston plate 35, and the piston air inlets 34 are all connected to an air inlet check valve. A plurality of piston air outlets 36 are provided on the bottom wall of the tank body 1, and the piston air outlets 36 are all connected to an air outlet check valve. The center bolt of the piston plate 35 is fixedly connected to a sliding column 31 slidably connected to the bottom surface of the tank body 1, and the side bolts of the sliding column 31 are fixedly connected to an L-shaped The connecting rod 32 is fixedly connected with a cylindrical pin 30 by bolts on the upper part of the connecting rod 32. A cylindrical sheave 28 is coaxially provided at the lower end of the main shaft 2. The side wall of the sheave 28 is provided with an annular groove 29 for the sliding of the cylindrical pin 30. The sliding column 31 is vertically slidably matched with the rotating sleeve 33. In this embodiment, a wear-resistant sleeve 19 is also fixedly bonded to the sliding match between the sliding column 31 and the rotating sleeve 33. The wear-resistant sleeve 19 can greatly reduce the wear of the sliding column 31 and increase the service life of the sliding column 31.

[0061] The axis of the sliding post 31 is in a coincident state or parallel state with the axis of the main shaft 2. Such a design can reduce the difficulty of manufacturing the device. In this embodiment, the axis of the sliding post 31 is in a coincident state with the axis of the main shaft 2.

[0062] like Figure 7 As shown, it also includes a plurality of turning blades 24 , which all pass through the lower portion of the adjacent stirring rod 22 and are welded thereto; the turning blades 24 are all X-shaped with the adjacent stirring rod 22 .

[0063] like Figure 1 、 Figure 2 、 Figure 7 and Figure 11 As shown, the bottom of the tank 1 is welded with several legs 16 and connected to a discharge pipe 11. A dust cover 14 is hingedly connected to the feed port. The legs 16 support the entire device and improve its overall stability. After the solid culture medium is transferred, the feed port is promptly closed with the dust cover 14 to reduce the chance of external dust contamination of the tank 1. After the microbial culture is completed, the microorganisms and materials in the tank 1 can be removed through the discharge pipe 11.

[0064] In this embodiment, a pressure gauge 15 is also bolted to the top of the tank body 1 to monitor the pressure inside the tank body 1 and improve the safety of the equipment. If the pressure inside the tank body 1 is too high (close to or exceeds the set value), the pressure can be released by opening the feed port or the infusion port.

[0065] The specific implementation process is as follows: First, solid culture medium and nutrient solution are input into the tank body 1 through the feed port and the infusion port, and then the reduction motor is started to drive the main shaft 2, the support rod, the guide sleeve 25 and the stirring rod 22 to rotate around the axis of the main shaft 2; at the same time, the gear 26 will rotate along the gear ring 27, thereby driving the stirring rod 22 to rotate, and driving the stirring blade 23 to rotate, thereby stirring the material.

[0066] When the stirring rod 22 rotates, the turning blade 24 will also revolve and rotate together with the stirring rod 22; when the turning blade 24 rotates around the main shaft 2 together with the stirring rod 22, since the turning blade 24 and the stirring rod 22 are X-shaped, the stirring range of the stirring rod 22 is greatly increased, and the comprehensiveness of the material stirring is effectively improved; when the turning blade 24 rotates with the rotation of the stirring rod 22, the turning blade 24 will stir the material near the stirring rod 22. Since the turning blade 24 is tilted, a tornado-like water flow will be formed on both sides of the turning blade 24, so that the material near the stirring blade 23 is evenly stirred, thereby improving the uniformity of the material stirring.

[0067] During the stirring process, the stirring rod 22 drives the stirring ring 5 to rotate around the rotating sleeve 33, and at the same time drives the scraper 51 and the rotating sleeve 33 to rotate, so that the scraper 51 cleans, stirs and pushes the material at the bottom of the tank body 1 upward.

[0068] During the stirring process, some materials will accumulate in the grinding trough 52. When the stirring rod 22 is driven, the bottom of the stirring rod 22 will grind and crush the materials in the grinding trough 52, and the materials will be discharged from the grinding trough 52 through the discharge hole 53, so as to avoid the materials from clogging the grinding trough 52 and affecting the rotation of the stirring rod 22. At the same time, it can also reduce the particle size of the materials, thereby improving the stirring effect of the materials.

[0069] The rotation of the main shaft 2 will drive the groove wheel 28 to rotate. Due to the limitation of the annular groove 29, the cylindrical pin 30 will move back and forth in the annular groove 29, thereby driving the connecting rod 32 and the sliding column 31 to slide back and forth vertically, so that the piston plate 35 can slide back and forth vertically in the piston chamber 3, continuously inputting external gas from the air inlet check valve into the piston chamber 3, and then discharging it into the tank body 1 through the air outlet check valve. The ejector 12 at the top of the tank body 1 is used to transport gas from top to bottom to the microorganisms in the tank body 1, and then the movement of the piston plate 35 is used to transport gas from bottom to top to the microorganisms in the tank body 1, thereby ensuring that the gas inside the tank body 1 is sufficient and evenly distributed, thereby optimizing the culture environment of the microorganisms.

[0070] In the prior art, the material is mainly loaded into the mixing box in the prior art, and the mixing shaft 61 and the mixing blade 23 are driven by the motor to rotate, thereby stirring the material. This method can often only stir the material in the middle of the mixing box, resulting in insufficient comprehensiveness and uniformity of the stirring, making the material mixing uneven, seriously affecting the culture effect of the microorganisms; in this embodiment, through the design of the gear 26 and the gear ring 27, the stirring shaft 61 can rotate while revolving. The rotation of the stirring shaft 61 can drive the mixing blade 23 and the turning blade 24 to rotate, thereby generating a stronger shear force, further improving the stirring effect of the material, and the revolution and rotation are carried out simultaneously, which not only ensures the stirring range, but also ensures the stirring intensity; compared with the stirring shaft 61 that only rotates in the prior art, the design of this embodiment greatly improves the uniformity and comprehensiveness of the material mixing, so that the material can be evenly mixed, thereby providing a good environment for the subsequent culture of microorganisms, and improving the success rate of microorganism culture. Example 2:

[0071] As attached Figure 2 、 Figure 3 、 Figure 5 、 Figure 7 and Figure 9 As shown, unlike the above embodiment, a bulk material cover 4 is provided on the bolt fixing sleeve of the main shaft 2, and the bulk material cover 4 is located above the gear ring 27 and below the feed port and the infusion port; the bulk material cover 4 is conical, and a plurality of bulk material holes 41 are opened on the bulk material cover 4, and a plurality of raised ridges 42 are welded on the top of the bulk material cover 4.

[0072] The specific implementation process is as follows: after the material passes through the feed port and the infusion port, it will flow to the top of the bulk cover 4 and be dispersed through the bulk holes 41, so as to be evenly scattered to all parts of the tank body 1. At the same time, the rotation of the main shaft 2 will drive the bulk cover 4 and the convex rib 42 to rotate synchronously. Since the bulk cover 4 is conical, the material will flow along the edge of the convex rib 42 to the edge of the bulk cover 4 under the action of centrifugal force, and then be input into the tank body 1, thereby improving the utilization rate of the material.

[0073] The ridges 42 not only guide the materials but also serve as reinforcement ribs for the bulk material cover 4 , thereby increasing the structural strength of the bulk material cover 4 and prolonging the service life of the bulk material cover 4 . Example 3:

[0074] like Figure 7 Combine Figure 17 As shown, different from the above embodiment, a protective frame 6 is provided on the sheave 28 for preventing the material from clogging the annular groove 29, and the top of the protective frame 6 is conical; a plurality of stirring shafts 61 are fixedly connected to the outer wall of the protective frame 6 with bolts.

[0075] The specific implementation process is as follows: the protective frame 6 can isolate the material from the groove wheel 28 to prevent the material from clogging the annular groove 29, thereby improving the overall feasibility of the device. The conical design of the top of the protective frame 6 can guide the material so that the material flows to the bottom of the tank body 1, avoiding the accumulation of material on the top of the protective frame 6 and improving the utilization rate of the material.

[0076] When the main shaft 2 drives the sheave 28 to rotate, the sheave 28 will drive the protective frame 6 and the stirring shaft 61 to rotate. The material on the protective frame 6 will separate from the protective frame 6 due to the centrifugal effect, thereby further improving the utilization rate of the material; at the same time, the stirring shaft 61 will further stir the material in the center of the tank body 1 to improve the uniformity of the material stirring.

[0077] When the stirring shaft 61 and the stirring rod 22 stir synchronously, most of the material near the stirring shaft 61 will be dispersed to the edge of the tank body 1 due to centrifugal force, and thus stirred by the stirring rod 22, while most of the material around the stirring rod 22 will be concentrated to the center of the tank body 1 due to centrifugal force, and thus stirred by the stirring shaft 61. The two work together to ensure that the materials at the center and edge positions of the tank body 1 are evenly stirred, thereby greatly improving the comprehensiveness and uniformity of material stirring, making the material more evenly distributed in the tube body, optimizing the culture environment of microorganisms, and thereby improving the success rate and culture quality of microorganism culture.

[0078] Obviously, the above embodiments are merely examples for clarity of explanation and are not intended to limit the implementation methods. Those skilled in the art will readily appreciate that other variations or modifications based on the above descriptions are possible. It is not necessary and impossible to enumerate all implementation methods here. Obvious variations or modifications arising therefrom remain within the scope of protection of the present invention.

Claims

1. A biotechnology culture device suitable for microorganisms, characterized in that: The invention comprises a tank body (1) and a main shaft (2), wherein the top of the tank body (1) is provided with a feed port and an infusion port, and the bottom of the tank body (1) is provided with a discharge port (17); the bottom of the main shaft (2) extends into the tank body (1) and rotates with the tank body (1); a driving mechanism for driving the main shaft (2) to rotate is provided on the top; and a plurality of stirring modules for stirring materials are provided in the tank body (1); The stirring module comprises a stirring rod (22), a guide sleeve (25) and a support rod; one end of the support rod is fixedly connected to the lower end of the main shaft (2), and the other end of the support rod is fixedly connected to the guide sleeve (25); a guide hole is opened on the guide sleeve (25); the stirring rod (22) passes through the guide hole and is rotatably connected to the guide sleeve (25); the stirring rod (22) is fixedly connected to a gear (26) and a plurality of stirring blades (23) in sequence from top to bottom along its axial direction; The distance between the upper end of the stirring rod (22) and the main shaft (2) is smaller than the distance between the lower end of the stirring rod (22) and the main shaft (2); a gear ring (27) is fixedly connected to the middle of the inner wall of the tank body (1), and the gears (26) are all engaged with the gear ring (27); and a gas supply mechanism for conveying gas into the tank body (1) is installed on the top of the tank body (1); A stirring ring (5) is provided in the tank body (1), and a plurality of grinding grooves (52) are provided on the top of the stirring ring (5). The bottoms of the stirring rods (22) extend to the bottoms of the adjacent grinding grooves (52). The inner bottom wall of the tank body (1) is rotatably connected to a rotating sleeve (33). The outer side wall of the rotating sleeve (33) is fixedly connected to a plurality of scrapers (51). The ends of the scrapers (51) away from the rotating sleeve (33) are fixedly connected to the stirring ring (5); the bottoms of the scrapers (51) are in contact with the inner bottom wall of the tank body (1); the scrapers (51) are inclined; the bottoms of the scrapers (51) are conical; the lower ends of the stirring rods (22) are ellipsoidal; and the bottoms of the grinding grooves (52) are provided with discharge holes (53). It also includes a plurality of material turning blades (24), each of which passes through the lower portion of the adjacent stirring rod (22) and is fixedly connected thereto; and each of the material turning blades (24) forms an X shape with the adjacent stirring rod (22).

2. The biotechnology culture device for microorganisms according to claim 1, characterized in that: A piston chamber (3) is fixedly connected to the bottom of the tank body (1), and a piston plate (35) is vertically slidably fitted in the piston chamber (3). A plurality of piston air inlets (34) are provided on the piston plate (35), and the piston air inlets (34) are all connected to an air inlet check valve. A plurality of piston air outlets (36) are provided on the bottom wall of the tank body (1), and the piston air outlets (36) are all connected to an air outlet check valve. A sliding column (31) is fixedly connected to the center of the piston plate (35) and is slidably connected to the bottom surface of the tank body (1). An L-shaped connecting rod (32) is fixedly connected to the side of the sliding column (31), and a cylindrical pin (30) is fixedly connected to the upper part of the connecting rod (32). A cylindrical groove wheel (28) is coaxially provided at the lower end of the main shaft (2), and an annular groove (29) for sliding of the cylindrical pin (30) is provided on the side wall of the groove wheel (28); the sliding column (31) is vertically slidably fitted with the rotating sleeve (33).

3. The biotechnology culture device for microorganisms according to claim 2, characterized in that: The axis of the sliding column (31) and the axis of the main shaft (2) are in one of a coincident state or a parallel state.

4. The biotechnology culture device for microorganisms according to claim 3, characterized in that: A bulk material cover (4) is fixedly sleeved on the main shaft (2), and the bulk material cover (4) is located above the gear ring (27) and below the feed port and the infusion port; the bulk material cover (4) is conical, and has a plurality of bulk material holes (41) formed on the bulk material cover (4), and a plurality of raised ridges (42) are fixedly connected to the top of the bulk material cover (4).

5. The biotechnology culture device for microorganisms according to claim 4, characterized in that: The bottom of the tank body (1) is fixedly connected to a plurality of legs (16), the bottom of the tank body (1) is connected to a discharge pipe (11), and a dust cover (14) is hingedly connected to the feed port; a protective frame (6) is fixedly sleeved on the groove wheel (28) for preventing the material from clogging the annular groove (29), and the top of the protective frame (6) is conical; the outer wall of the protective frame (6) is fixedly connected to a plurality of stirring shafts (61).

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